Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0007674

Physical Location & Seq

Genomic Coordinates Forward (+)

18261307 .. 18265174

Gene Structure 3868 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g14690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGGCCAACTTCTTCATCTTCCTCCTGCTCTTCTTATCGTCTCCACTCACCGCATCTCCAAGGCCAAACCATAGAGTCCTCCACCAGCCATTTCTGCCGCAGGAATCTTCTTCGCCTCCATCTCCCTCACCGCCTTCCCCACCTCCGAACCCAACTCCCCCAGTTTCCAGTTCCAACTCTCCCCCTCCCAATTCCCCTTTTTTCCCATCCTTACCCTCCCCTCCGCCTCCCCCTCCCTCTCCAACCACTGCCTTCGCCTCCTTCCCCGCCAACATCTCCTCCCTCATTGCCCCCCAGCCCACCAAAAACTCCAGCGCCCACAAGCTCCACGCCCTCACTATCGCCGCCGTGCTCTCCGCCGTCGTCGTCGTTTCCGTCGCCGCATTCTTCCTCTACCGTCGCAGACACCACAACGGCCGCAACTTCCCGGACAACGAGGACGAAAAGTCGTATGTTAGATCCGAGCACAGCAGCAGGCTGTTTCAGGCGCCCAATCTCAGCCACTCCTTCAGTGGTACACACAAGGTCCGAACCGGCTCTTCCAACAGCTCCGAGTTCCTCTACCTGGGCACAGTGGTCAACTCGCGTGGACTCGAGGAACGAGTTGACGCGCGCGGGAGCGTTGGTCGTAGGGCCGAAAAAGAAACTCGGAAAGTCGACTCACCGGACCTCCAGCCGCTTCCGCCGCTTGCTCGGCAAACCTCGAGGCTGAGCCGTTGGGAGAATGCCGAACCGGTTCTGACCCATGACCGAGACGATGAGGAAGAGGAAGAGGAAGAAGAGTTCTACTCTCCCAGAGGGTCTTCGGGCGATCGCGACAGCTCCAACGGAAACGGATCCGGGTCCAGGAGAGTATTCGCGGCGGTTGCGGGTGGCGTATTTGATGAAAGAGGCAGCGAAACGACGTCGTGTTCGTGCTCGTCTTCCGAATTGGGTTCACCGTCTCGGTCACACTCAATCAGTCTCTCGCCTCCGGTGAGTTTAAGTCCCAACAGAAGATCAGAAGAGCCCAAACCGCCGGAACCCTCAGCCATGCATTACACGGCGCCGTTTGCTGATGTCAATCTACGGTCGCCGTCGTTGACTCCAATGTCTTCCCCGGAGAGAGTTGTTGATGATTTCCCATCTGCATCGGACCGAAACGGAGGGTCTCCGTCGATGTTCTCGCTATCTTCGTCGCCGGAGAGAGGATTGGAGAAGACCCCTGATGTACCGGCGAAAGTTTCGGTCGTTCCGGATCAAAGTTCGCCGATTTCCTCACCGGAGAGAGATTTCGGAAGCAACCAACATGCATCATCGAAAGTTTCGGTGGTTTCTGATCAAAGTTCGCCGATTTCCTCACCGGAATTAAATTTCAGAAACAAACCAGATGCGTTACCGAAAGTTTCGGCCTTTTCAGATCGGAACGATCAATCCCCTTCACCATCTTTAAATTGTTCATTGTCACCAGAGAGAGAATCGAACGTTTCTGATTCAAGAGCCAAGTCCTTTTCACCTTCTATGTCACCACTGAGAGGTTTGGAGAAGAGCTCAGCTGCCTCACCAAGAATTTCAAATGCTTCGGATCGAGCATTTATTCATTTGGATCCTAAAATGCAGTCTCTTTCATCATCTTCATCGTCTTCGTTGTCGAATTCACCGGAGAGAGAGTTTAGTGGTGATTTAAAGCAGCCTCTTTCGGTTCCTCCGCCACCGCCAATGCCTCCATCACTGAGACTCTGGGAGAAACCAAGGCCTAGAACACCAGTTGGGCAAGTGATCTCAAGGCCTCCAGCTCTCATACCTCCTTCAAGACCTTTTGTGTTTCAAAACCCGGGAAAGGTCTCTGTTTCGCCGGTGAAGTTGACTCCAAGTTCAACACCATTGGAGCCGATTGAGGAGACTCCGAAACCAAAGTTGAAGCCATTGCATTGGGATAAAGTTAGAGCAAGCTCCGATCGCGAAATGGTGTGGGATCAGTTTAGATCGAGCTCTTTCAAATTGAATGAAGAAATGATAGAGACATTGTTTGTTGTAAAAACACCAAACCCGAATCCAAGGGAAACGACACCACGTGCAGTTCTTCCCTCACCAAATCAAGAGAATAGAGTACTTGATCCAAAAAAGTCGCAGAATATTGCAATCTCACTAAGGGCTCTCAATGTGACCATAGAGGAAGTTTGTGAAGCTTTATTAGAAGGAAACTCGGACGCTCTTGGAACTGAGTTACTTGAAAGTCTAATGAAGATGGCTCCAACCAAAGAAGAAGAACGGAAGCTGAAGGAATACAAAGACGACTCACCAGTTAACCTTGGTCCTGCTGAGAGATTTCTGAAAGAGCTGCTCGACGTTCCTTTTGCATTTAAAAGGATTGATGCAATGCTTTACATGACTAATTTTGAGTCTGAGATCGAGTACCTTAAAAAGTCGTTTGAAACTTTGGAGGCTGCCTGTGAAGAATTGAGAAACAGCAGGATGTTCTTGAAGCTTCTGGAAGCTGTTCTGAAGACTGGAAACCGTATGAATGTTGGGACAAACCGTGGTGATGCCCAAGCCTTCAAACTGGACACACTCCTCAAGCTGGTTGACGTCAAGGGGGCAGATGGGAAAACCACACTCTTGCATTTTGTCGTACAAGAAATCATAAGGACGGAAGGTGCTCGTCTCAACGGTGGCAATCAAACTTCAAACCCAACTTTGAGTGATGATGTCAAGTGCAGGAGGCTTGGCCTGCAAGTTGTTTCAGCTCTCAGTTCAGACCTCAGTAATGTGAAGAAAGCTGCTGTCATGGATTCTGATGTGCTTAGCAGTGATGTTTCCAAACTTTCCAAAGGAATTAGCAACATTGCGGAGGTTGTGCAATTAAATGAGACGACGGTGTCAGATGACAGCAGCCGGAAATTCTCAGAGTCGATGAACATGTTCATGAAATTGGCTGAAGAGGAGATTATAAGACTTCAAGCTCAAGAAAGTGTTGCATTGTCCCTGGTGAAGGAGATTACAGAGTACTTCCACGGGAACTCCGCTAGGGAAGAAGCTCACCCGTTTAGAATCTTCATGGTGGTCAGAGACTTTCTTACAGTTCTTGATCGGGTATGTAAGGAAGTTGGTTCAATAAATGAGCGAACCCAAGTTAGTACCGCCCACAAATTTCCAGTTCCAGTAAATCCAATGCTACCGCAAGTAGTTCCAGTAAATCCAATGCTGCCACAACTAGTTCCTGGAATTCATGGGTTGAGGCGGTACAGTTCATCCGATGATGAGAGCGCGTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

1086

Amino Acids

118.85

Weight (kDa)

6.28

Isoelectric Point (pI)

71.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	627 - 1022	1.3e-121	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25500
fragaria_vesca	FvH4_2g17360 FvH4_2g17360 FvH4_2g17360
malus_domestica	MD05G1152500.v1.1 MD10G1147000.v1.1
prunus_persica	Prupe.8G185400_v2.0.a1
pyrus_communis	pycom05g14690 pycom10g12670
rosa_chinensis	RchiOBHm_Chr6g0281991
rosa_laevigata	RLG00000012921
rosa_multiflora	Rmu_sc0000569.1_g000001 Rmu_sc0001255.1_g000001 Rmu_sc0011823.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186650
rosa_rugosa	Rorug06G0144700
rosa_samantha	Rh4CG162200 Rh4CG270300 Rh6AG257900 Rh6BG260900 Rh6CG259800 Rh6DG252200 Rh7AG416400
rosa_wichuraiana	Rw6G022300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2930
AasI	GACNNNNNNGTC	2 cut(s)	1090, 2857
AatII	GACGTC	2 cut(s)	908, 2571
AbsI	CCTCGAGG	1 cut(s)	703
AccB1I	GGYRCC	2 cut(s)	487, 1045
AccI	GTMKAC	1 cut(s)	657
AccII	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
AccIII	TCCGGA	1 cut(s)	1234
AclI	AACGTT	1 cut(s)	1464
AclWI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 1962, 2090
AcoI	YGGCCR	2 cut(s)	3, 415
AcsI	RAATTY	6 cut(s)	1351, 1548, 1633, 2879, 3128, 3204
AcuI	CTGAAG	4 cut(s)	493, 2279, 2504, 2937
AcvI	CACGTG	1 cut(s)	2054
AcyI	GRCGYC	4 cut(s)	488, 905, 1046, 2568
AdeI	CACNNNGTG	1 cut(s)	2054
AfaI	GTAC	8 cut(s)	517, 1212, 2091, 2396, 2613, 2987, 3118, 3224
AfiI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1819, 2560, 2971
AflIII	ACRYGT	1 cut(s)	2898
AgeI	ACCGGT	1 cut(s)	733
AhdI	GACNNNNNGTC	1 cut(s)	445
AhlI	ACTAGT	1 cut(s)	3193
AjnI	CCWGG	4 cut(s)	564, 845, 2964, 3199
AloI	GAACNNNNNNTCC	4 cut(s)	767, 799, 1397, 1429
Alw21I	GWGCWC	6 cut(s)	354, 468, 920, 1532, 1973, 2641
AlwI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 1962, 2090
Ama87I	CYCGRG	3 cut(s)	593, 703, 1813
Aor13HI	TCCGGA	1 cut(s)	1234
AoxI	GGCC	8 cut(s)	3, 62, 415, 633, 1389, 1733, 1766, 2707
ApeKI	GCWGC	9 cut(s)	471, 894, 1535, 1669, 2320, 2426, 2759, 2871, 3184
ApoI	RAATTY	6 cut(s)	1351, 1548, 1633, 2879, 3128, 3204
ArsI	GACNNNNNNTTYG	4 cut(s)	895, 927, 2318, 2350
AsiGI	ACCGGT	1 cut(s)	733
AsiSI	GCGATCGC	1 cut(s)	814
Asp700I	GAANNNNTTC	3 cut(s)	735, 2478, 2900
AspLEI	GCGC	6 cut(s)	317, 490, 613, 615, 1048, 3248
AspS9I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2294
AsuC2I	CCSGG	5 cut(s)	428, 841, 1100, 1814, 1815
AvaI	CYCGRG	3 cut(s)	593, 703, 1813
AvaII	GGWCC	5 cut(s)	526, 667, 843, 1135, 2294
BaeGI	GKGCMC	1 cut(s)	572
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	836, 1585
BanI	GGYRCC	2 cut(s)	487, 1045
BanII	GRGCYC	4 cut(s)	1011, 1532, 1973, 2137
BbrPI	CACGTG	1 cut(s)	2054
BbsI	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2492
Bbv12I	GWGCWC	6 cut(s)	354, 468, 920, 1532, 1973, 2641
BbvCI	CCTCAGC	1 cut(s)	1027
BbvI	GCAGC	9 cut(s)	483, 906, 1522, 1681, 2307, 2413, 2746, 2883, 3171
BccI	CCATC	7 cut(s)	127, 214, 1132, 1432, 1714, 2221, 2576
BceAI	ACGGC	7 cut(s)	332, 344, 430, 699, 1033, 1059, 1060
BciT130I	CCWGG	4 cut(s)	566, 847, 2966, 3201
BclI	TGATCA	1 cut(s)	1318
BcnI	CCSGG	5 cut(s)	428, 841, 1100, 1814, 1815
BcuI	ACTAGT	1 cut(s)	3193
BfaI	CTAG	3 cut(s)	1737, 3006, 3194
BfoI	RGCGCY	3 cut(s)	318, 491, 1049
BlpI	GCTNAGC	3 cut(s)	710, 1531, 2783
BmcAI	AGTACT	2 cut(s)	2091, 2987
Bme1390I	CCNGG	9 cut(s)	428, 566, 841, 847, 1100, 1814, 1815, 2966, 3201
Bme18I	GGWCC	5 cut(s)	526, 667, 843, 1135, 2294
BmeRI	GACNNNNNGTC	1 cut(s)	445
BmeT110I	CYCGRG	3 cut(s)	593, 703, 1813
BmgT120I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2294
BmiI	GGNNCC	9 cut(s)	489, 838, 844, 1023, 1047, 1587, 1683, 1869, 2232
BmrFI	CCNGG	9 cut(s)	428, 566, 841, 847, 1100, 1814, 1815, 2966, 3201
BmrI	ACTGGG	1 cut(s)	155
BmsI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2344, 2515
BmuI	ACTGGG	1 cut(s)	155
BpiI	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2492
BplI	GAGNNNNNCTC	5 cut(s)	773, 805, 1514, 1546, 3235
BpmI	CTGGAG	3 cut(s)	295, 656, 1755
Bpu10I	CCTNAGC	1 cut(s)	1027
Bpu1102I	GCTNAGC	3 cut(s)	710, 1531, 2783
BpuEI	CTTGAG	3 cut(s)	1747, 2540, 2928
BpuMI	CCSGG	5 cut(s)	428, 841, 1100, 1814, 1815
BsaAI	YACGTR	1 cut(s)	2054
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaHI	GRCGYC	4 cut(s)	488, 905, 1046, 2568
BsaI	GGTCTC	2 cut(s)	1155, 1828
BsaWI	WCCGGW	7 cut(s)	664, 733, 973, 1234, 1261, 1342, 1639
Bsc4I	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1819, 2560, 2971
Bse118I	RCCGGY	4 cut(s)	533, 733, 1213, 1834
Bse1I	ACTGG	9 cut(s)	161, 168, 1745, 2282, 2494, 2547, 3134, 3140, 3170
Bse3DI	GCAATG	4 cut(s)	285, 1904, 2364, 2823
Bse8I	GATNNNNATC	1 cut(s)	1062
BseAI	TCCGGA	1 cut(s)	1234
BseBI	CCWGG	4 cut(s)	566, 847, 2966, 3201
BseGI	GGATG	3 cut(s)	206, 2460, 3230
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1819, 2560, 2971
BseMI	GCAATG	4 cut(s)	285, 1904, 2364, 2823
BseNI	ACTGG	9 cut(s)	161, 168, 1745, 2282, 2494, 2547, 3134, 3140, 3170
BsePI	GCGCGC	1 cut(s)	611
BseRI	GAGGAG	4 cut(s)	268, 1892, 2543, 2936
BseSI	GKGCMC	1 cut(s)	572
BseX3I	CGGCCG	1 cut(s)	415
BseXI	GCAGC	9 cut(s)	483, 906, 1522, 1681, 2307, 2413, 2746, 2883, 3171
BseYI	CCCAGC	1 cut(s)	294
BsgI	GTGCAG	2 cut(s)	2076, 2716
Bsh1236I	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
Bsh1285I	CGRYCG	5 cut(s)	418, 814, 1073, 1230, 1939
BshFI	GGCC	8 cut(s)	5, 64, 417, 635, 1391, 1735, 1768, 2709
BshNI	GGYRCC	2 cut(s)	487, 1045
BshTI	ACCGGT	1 cut(s)	733
BsiEI	CGRYCG	5 cut(s)	418, 814, 1073, 1230, 1939
BsiHKAI	GWGCWC	6 cut(s)	354, 468, 920, 1532, 1973, 2641
BsiHKCI	CYCGRG	3 cut(s)	593, 703, 1813
BslFI	GGGAC	3 cut(s)	972, 2524, 2947
BslI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1819, 2560, 2971
BsmBI	CGTCTC	5 cut(s)	45, 747, 948, 2648, 2843
BsmFI	GGGAC	3 cut(s)	972, 2524, 2947
BsmI	GAATGC	2 cut(s)	383, 730
BsnI	GGCC	8 cut(s)	5, 64, 417, 635, 1391, 1735, 1768, 2709
Bso31I	GGTCTC	2 cut(s)	1155, 1828
BsoBI	CYCGRG	3 cut(s)	593, 703, 1813
Bsp1286I	GDGCHC	9 cut(s)	354, 468, 572, 920, 1011, 1532, 1973, 2137, 2641
Bsp13I	TCCGGA	1 cut(s)	1234
Bsp1720I	GCTNAGC	3 cut(s)	710, 1531, 2783
Bsp68I	TCGCGA	2 cut(s)	816, 1941
BspANI	GGCC	8 cut(s)	5, 64, 417, 635, 1391, 1735, 1768, 2709
BspEI	TCCGGA	1 cut(s)	1234
BspFNI	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
BspHI	TCATGA	1 cut(s)	2904
BspLI	GGNNCC	9 cut(s)	489, 838, 844, 1023, 1047, 1587, 1683, 1869, 2232
BspPI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 1962, 2090
BspQI	GCTCTTC	4 cut(s)	35, 544, 999, 1520
BspT107I	GGYRCC	2 cut(s)	487, 1045
BspTNI	GGTCTC	2 cut(s)	1155, 1828
BsrDI	GCAATG	4 cut(s)	285, 1904, 2364, 2823
BsrFI	RCCGGY	4 cut(s)	533, 733, 1213, 1834
BsrI	ACTGG	9 cut(s)	161, 168, 1745, 2282, 2494, 2547, 3134, 3140, 3170
BssAI	RCCGGY	4 cut(s)	533, 733, 1213, 1834
BssHII	GCGCGC	1 cut(s)	611
BssNI	GRCGYC	4 cut(s)	488, 905, 1046, 2568
BssT1I	CCWWGG	4 cut(s)	59, 1730, 2036, 2290
Bst2UI	CCWGG	4 cut(s)	566, 847, 2966, 3201
Bst6I	CTCTTC	8 cut(s)	35, 544, 759, 765, 774, 999, 1520, 2913
BstACI	GRCGYC	4 cut(s)	488, 905, 1046, 2568
BstBAI	YACGTR	1 cut(s)	2054
BstC8I	GCNNGC	5 cut(s)	476, 613, 690, 1930, 2711
BstDSI	CCRYGG	2 cut(s)	2518, 2992
BstENI	CCTNNNNNAGG	1 cut(s)	2969
BstF5I	GGATG	3 cut(s)	206, 2460, 3230
BstFNI	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
BstH2I	RGCGCY	3 cut(s)	318, 491, 1049
BstHHI	GCGC	6 cut(s)	317, 490, 613, 615, 1048, 3248
BstMCI	CGRYCG	5 cut(s)	418, 814, 1073, 1230, 1939
BstMWI	GCNNNNNNNGC	9 cut(s)	94, 682, 685, 694, 866, 1015, 1052, 1938, 2710
BstNI	CCWGG	4 cut(s)	566, 847, 2966, 3201
BstNSI	RCATGY	2 cut(s)	1292, 2902
BstSCI	CCNGG	9 cut(s)	426, 564, 839, 845, 1098, 1812, 1813, 2964, 3199
BstSLI	GKGCMC	1 cut(s)	572
BstUI	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
BstV1I	GCAGC	9 cut(s)	483, 906, 1522, 1681, 2307, 2413, 2746, 2883, 3171
BstV2I	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2492
BstX2I	RGATCY	3 cut(s)	458, 836, 1585
BstXI	CCANNNNNNTGG	1 cut(s)	1911
BstYI	RGATCY	3 cut(s)	458, 836, 1585
BstZI	CGGCCG	1 cut(s)	415
BsuRI	GGCC	8 cut(s)	5, 64, 417, 635, 1391, 1735, 1768, 2709
BtgI	CCRYGG	2 cut(s)	2518, 2992
BtsCI	GGATG	3 cut(s)	206, 2460, 3230
BtsI	GCAGTG	2 cut(s)	246, 2794
BtsIMutI	CAGTG	6 cut(s)	246, 517, 579, 1508, 1709, 2794
BtuMI	TCGCGA	2 cut(s)	816, 1941
Cac8I	GCNNGC	5 cut(s)	476, 613, 690, 1930, 2711
CciI	TCATGA	1 cut(s)	2904
CfoI	GCGC	6 cut(s)	317, 490, 613, 615, 1048, 3248
Cfr10I	RCCGGY	4 cut(s)	533, 733, 1213, 1834
Cfr13I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2294
Cfr9I	CCCGGG	1 cut(s)	1813
CpoI	CGGWCCG	1 cut(s)	1135
CseI	GACGC	3 cut(s)	617, 2198, 3237
Csp6I	GTAC	8 cut(s)	516, 1211, 2090, 2395, 2612, 2986, 3117, 3223
CspAI	ACCGGT	1 cut(s)	733
CspCI	CAANNNNNGTGG	2 cut(s)	568, 603
CspI	CGGWCCG	1 cut(s)	1135
CviAII	CATG	9 cut(s)	746, 1033, 1289, 2368, 2767, 2899, 2905, 3037, 3209
CviQI	GTAC	8 cut(s)	516, 1211, 2090, 2395, 2612, 2986, 3117, 3223
DinI	GGCGCC	2 cut(s)	489, 1047
DraI	TTTAAA	3 cut(s)	1431, 1665, 2344
DraIII	CACNNNGTG	1 cut(s)	2054
DrdI	GACNNNNNNGTC	2 cut(s)	1090, 2857
DriI	GACNNNNNGTC	1 cut(s)	445
DseDI	GACNNNNNNGTC	2 cut(s)	1090, 2857
EaeI	YGGCCR	2 cut(s)	3, 415
EagI	CGGCCG	1 cut(s)	415
Eam1104I	CTCTTC	8 cut(s)	35, 544, 759, 765, 774, 999, 1520, 2913
Eam1105I	GACNNNNNGTC	1 cut(s)	445
EarI	CTCTTC	8 cut(s)	35, 544, 759, 765, 774, 999, 1520, 2913
EciI	GGCGGA	4 cut(s)	213, 346, 672, 1677
Ecl136II	GAGCTC	2 cut(s)	1530, 1971
EclXI	CGGCCG	1 cut(s)	415
Eco130I	CCWWGG	4 cut(s)	59, 1730, 2036, 2290
Eco147I	AGGCCT	2 cut(s)	1735, 1768
Eco24I	GRGCYC	4 cut(s)	1011, 1532, 1973, 2137
Eco31I	GGTCTC	2 cut(s)	1155, 1828
Eco47I	GGWCC	5 cut(s)	526, 667, 843, 1135, 2294
Eco52I	CGGCCG	1 cut(s)	415
Eco53kI	GAGCTC	2 cut(s)	1530, 1971
Eco57I	CTGAAG	4 cut(s)	493, 2279, 2504, 2937
Eco72I	CACGTG	1 cut(s)	2054
Eco88I	CYCGRG	3 cut(s)	593, 703, 1813
EcoICRI	GAGCTC	2 cut(s)	1530, 1971
EcoNI	CCTNNNNNAGG	1 cut(s)	2969
EcoRI	GAATTC	2 cut(s)	1633, 3204
EcoRII	CCWGG	4 cut(s)	564, 845, 2964, 3199
EcoT14I	CCWWGG	4 cut(s)	59, 1730, 2036, 2290
EcoT22I	ATGCAT	2 cut(s)	1038, 1294
EcoT38I	GRGCYC	4 cut(s)	1011, 1532, 1973, 2137
EgeI	GGCGCC	2 cut(s)	489, 1047
EheI	GGCGCC	2 cut(s)	489, 1047
ErhI	CCWWGG	4 cut(s)	59, 1730, 2036, 2290
Esp3I	CGTCTC	5 cut(s)	45, 747, 948, 2648, 2843
FaeI	CATG	9 cut(s)	749, 1036, 1292, 2371, 2770, 2902, 2908, 3040, 3212
FaqI	GGGAC	3 cut(s)	972, 2524, 2947
FatI	CATG	9 cut(s)	745, 1032, 1288, 2367, 2766, 2898, 2904, 3036, 3208
FauI	CCCGC	3 cut(s)	274, 608, 862
FbaI	TGATCA	1 cut(s)	1318
FblI	GTMKAC	1 cut(s)	657
FokI	GGATG	3 cut(s)	193, 2467, 3217
FriOI	GRGCYC	4 cut(s)	1011, 1532, 1973, 2137
FspBI	CTAG	3 cut(s)	1737, 3006, 3194
GlaI	GCGC	6 cut(s)	316, 489, 612, 614, 1047, 3247
GsaI	CCCAGC	1 cut(s)	298
GsuI	CTGGAG	3 cut(s)	295, 656, 1755
HaeII	RGCGCY	3 cut(s)	318, 491, 1049
HaeIII	GGCC	8 cut(s)	5, 64, 417, 635, 1391, 1735, 1768, 2709
HgaI	GACGC	3 cut(s)	617, 2198, 3237
HhaI	GCGC	6 cut(s)	317, 490, 613, 615, 1048, 3248
Hin1I	GRCGYC	4 cut(s)	488, 905, 1046, 2568
Hin1II	CATG	9 cut(s)	749, 1036, 1292, 2371, 2770, 2902, 2908, 3040, 3212
Hin6I	GCGC	6 cut(s)	315, 488, 611, 613, 1046, 3246
HinP1I	GCGC	6 cut(s)	315, 488, 611, 613, 1046, 3246
HincII	GTYRAC	7 cut(s)	580, 607, 658, 1083, 1845, 2287, 2566
HindII	GTYRAC	7 cut(s)	580, 607, 658, 1083, 1845, 2287, 2566
HindIII	AAGCTT	2 cut(s)	2166, 2465
HpaI	GTTAAC	1 cut(s)	2287
HpyCH4IV	ACGT	5 cut(s)	905, 1464, 2053, 2328, 2568
HpyF10VI	GCNNNNNNNGC	9 cut(s)	94, 682, 685, 694, 866, 1015, 1052, 1938, 2710
HpySE526I	ACGT	5 cut(s)	905, 1464, 2053, 2328, 2568
Hsp92I	GRCGYC	4 cut(s)	488, 905, 1046, 2568
Hsp92II	CATG	9 cut(s)	749, 1036, 1292, 2371, 2770, 2902, 2908, 3040, 3212
HspAI	GCGC	6 cut(s)	315, 488, 611, 613, 1046, 3246
KasI	GGCGCC	2 cut(s)	487, 1045
Kpn2I	TCCGGA	1 cut(s)	1234
Ksp22I	TGATCA	1 cut(s)	1318
KspAI	GTTAAC	1 cut(s)	2287
LguI	GCTCTTC	4 cut(s)	35, 544, 999, 1520
LmnI	GCTCC	7 cut(s)	330, 554, 618, 827, 1867, 1937, 2236
Lsp1109I	GCAGC	9 cut(s)	483, 906, 1522, 1681, 2307, 2413, 2746, 2883, 3171
LweI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2344, 2515
MaeI	CTAG	3 cut(s)	1737, 3006, 3194
MaeII	ACGT	5 cut(s)	905, 1464, 2053, 2328, 2568
MaeIII	GTNAC	6 cut(s)	948, 1374, 1443, 1503, 2143, 2205
MauBI	CGCGCGCG	1 cut(s)	611
MflI	RGATCY	3 cut(s)	458, 836, 1585
MhlI	GDGCHC	9 cut(s)	354, 468, 572, 920, 1011, 1532, 1973, 2137, 2641
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	488, 1046
MmeI	TCCRAC	5 cut(s)	198, 266, 567, 849, 2258
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	1038, 1294
MroI	TCCGGA	1 cut(s)	1234
MroXI	GAANNNNTTC	3 cut(s)	735, 2478, 2900
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	8 cut(s)	983, 1349, 1430, 1664, 2286, 2343, 2400, 2843
MslI	CAYNNNNRTG	1 cut(s)	2903
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	1535
MspR9I	CCNGG	9 cut(s)	428, 566, 841, 847, 1100, 1814, 1815, 2966, 3201
Mva1269I	GAATGC	2 cut(s)	383, 730
MvaI	CCWGG	4 cut(s)	566, 847, 2966, 3201
MvnI	CGCG	8 cut(s)	586, 611, 613, 615, 816, 860, 1941, 3248
MwoI	GCNNNNNNNGC	9 cut(s)	94, 682, 685, 694, 866, 1015, 1052, 1938, 2710
NarI	GGCGCC	2 cut(s)	488, 1046
NciI	CCSGG	5 cut(s)	428, 841, 1100, 1814, 1815
NlaIII	CATG	9 cut(s)	749, 1036, 1292, 2371, 2770, 2902, 2908, 3040, 3212
NlaIV	GGNNCC	9 cut(s)	489, 838, 844, 1023, 1047, 1587, 1683, 1869, 2232
NmeAIII	GCCGAG	1 cut(s)	673
NmuCI	GTSAC	4 cut(s)	948, 1443, 1503, 2143
NruI	TCGCGA	2 cut(s)	816, 1941
NsiI	ATGCAT	2 cut(s)	1038, 1294
NspI	RCATGY	2 cut(s)	1292, 2902
PaeR7I	CTCGAG	2 cut(s)	593, 703
PagI	TCATGA	1 cut(s)	2904
PauI	GCGCGC	1 cut(s)	611
PceI	AGGCCT	2 cut(s)	1735, 1768
PciI	ACATGT	1 cut(s)	2898
PciSI	GCTCTTC	4 cut(s)	35, 544, 999, 1520
PcsI	WCGNNNNNNNCGW	3 cut(s)	372, 911, 1172
PctI	GAATGC	2 cut(s)	383, 730
PdmI	GAANNNNTTC	3 cut(s)	735, 2478, 2900
PfeI	GAWTC	6 cut(s)	104, 1457, 1472, 2032, 2771, 3030
PflFI	GACNNNGTC	1 cut(s)	2857
PfoI	TCCNGGA	3 cut(s)	426, 845, 3199
PinAI	ACCGGT	1 cut(s)	733
Ple19I	CGATCG	2 cut(s)	814, 1939
PluTI	GGCGCC	2 cut(s)	491, 1049
PmaCI	CACGTG	1 cut(s)	2054
PmlI	CACGTG	1 cut(s)	2054
Ppu21I	YACGTR	1 cut(s)	2054
PscI	ACATGT	1 cut(s)	2898
PsiI	TTATAA	1 cut(s)	2930
Psp124BI	GAGCTC	2 cut(s)	1532, 1973
Psp1406I	AACGTT	1 cut(s)	1464
Psp6I	CCWGG	4 cut(s)	564, 845, 2964, 3199
PspCI	CACGTG	1 cut(s)	2054
PspFI	CCCAGC	1 cut(s)	294
PspGI	CCWGG	4 cut(s)	564, 845, 2964, 3199
PspN4I	GGNNCC	9 cut(s)	489, 838, 844, 1023, 1047, 1587, 1683, 1869, 2232
PspPI	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2294
PspXI	VCTCGAGB	2 cut(s)	593, 703
PsuI	RGATCY	3 cut(s)	458, 836, 1585
PsyI	GACNNNGTC	1 cut(s)	2857
PteI	GCGCGC	1 cut(s)	611
PvuI	CGATCG	2 cut(s)	814, 1939
PvuII	CAGCTG	1 cut(s)	1535
RgaI	GCGATCGC	1 cut(s)	814
RruI	TCGCGA	2 cut(s)	816, 1941
RsaI	GTAC	8 cut(s)	517, 1212, 2091, 2396, 2613, 2987, 3118, 3224
RsaNI	GTAC	8 cut(s)	516, 1211, 2090, 2395, 2612, 2986, 3117, 3223
RseI	CAYNNNNRTG	1 cut(s)	2903
Rsr2I	CGGWCCG	1 cut(s)	1135
RsrII	CGGWCCG	1 cut(s)	1135
SacI	GAGCTC	2 cut(s)	1532, 1973
SalI	GTCGAC	1 cut(s)	656
SapI	GCTCTTC	4 cut(s)	35, 544, 999, 1520
SaqAI	TTAA	8 cut(s)	983, 1349, 1430, 1664, 2286, 2343, 2400, 2843
Sau96I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2294
ScaI	AGTACT	2 cut(s)	2091, 2987
ScrFI	CCNGG	9 cut(s)	428, 566, 841, 847, 1100, 1814, 1815, 2966, 3201
SduI	GDGCHC	9 cut(s)	354, 468, 572, 920, 1011, 1532, 1973, 2137, 2641
SfaAI	GCGATCGC	1 cut(s)	814
SfaNI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2344, 2515
SfoI	GGCGCC	2 cut(s)	489, 1047
Sfr274I	CTCGAG	2 cut(s)	593, 703
SgfI	GCGATCGC	1 cut(s)	814
SgrAI	CRCCGGYG	1 cut(s)	1834
SinI	GGWCC	5 cut(s)	526, 667, 843, 1135, 2294
SlaI	CTCGAG	2 cut(s)	593, 703
SmaI	CCCGGG	1 cut(s)	1815
SmiMI	CAYNNNNRTG	1 cut(s)	2903
SmlI	CTYRAG	5 cut(s)	593, 703, 1762, 2555, 2943
SmoI	CTYRAG	5 cut(s)	593, 703, 1762, 2555, 2943
SpeI	ACTAGT	1 cut(s)	3193
SseBI	AGGCCT	2 cut(s)	1735, 1768
SspDI	GGCGCC	2 cut(s)	487, 1045
SspI	AATATT	1 cut(s)	2116
SspMI	CTAG	3 cut(s)	1737, 3006, 3194
SstI	GAGCTC	2 cut(s)	1532, 1973
StuI	AGGCCT	2 cut(s)	1735, 1768
StyD4I	CCNGG	9 cut(s)	426, 564, 839, 845, 1098, 1812, 1813, 2964, 3199
StyI	CCWWGG	4 cut(s)	59, 1730, 2036, 2290
TaiI	ACGT	5 cut(s)	908, 1467, 2056, 2331, 2571
TaqII	GACCGA	4 cut(s)	765, 936, 1152, 1216
TatI	WGTACW	2 cut(s)	2089, 2985
TauI	GCSGC	7 cut(s)	100, 347, 383, 420, 679, 688, 863
TfiI	GAWTC	6 cut(s)	104, 1457, 1472, 2032, 2771, 3030
Tru1I	TTAA	8 cut(s)	983, 1349, 1430, 1664, 2286, 2343, 2400, 2843
Tru9I	TTAA	8 cut(s)	983, 1349, 1430, 1664, 2286, 2343, 2400, 2843
TscAI	CASTG	6 cut(s)	253, 517, 579, 1515, 1716, 2794
TseFI	GTSAC	4 cut(s)	948, 1443, 1503, 2143
TseI	GCWGC	9 cut(s)	471, 894, 1535, 1669, 2320, 2426, 2759, 2871, 3184
Tsp45I	GTSAC	4 cut(s)	948, 1443, 1503, 2143
TspGWI	ACGGA	7 cut(s)	364, 843, 849, 1143, 1158, 2266, 2645
TspMI	CCCGGG	1 cut(s)	1813
TspRI	CASTG	6 cut(s)	253, 517, 579, 1515, 1716, 2794
Tth111I	GACNNNGTC	1 cut(s)	2857
VpaK11BI	GGWCC	5 cut(s)	526, 667, 843, 1135, 2294
XagI	CCTNNNNNAGG	1 cut(s)	2969
XapI	RAATTY	6 cut(s)	1351, 1548, 1633, 2879, 3128, 3204
XceI	RCATGY	2 cut(s)	1292, 2902
XcmI	CCANNNNNNNNNTGG	1 cut(s)	509
XhoI	CTCGAG	2 cut(s)	593, 703
XmaI	CCCGGG	1 cut(s)	1813
XmiI	GTMKAC	1 cut(s)	657
XmnI	GAANNNNTTC	3 cut(s)	735, 2478, 2900
XspI	CTAG	3 cut(s)	1737, 3006, 3194
ZraI	GACGTC	2 cut(s)	906, 2569
ZrmI	AGTACT	2 cut(s)	2091, 2987
Zsp2I	ATGCAT	2 cut(s)	1038, 1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.