Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007674

Physical Location & Seq

Genomic Coordinates Forward (+)

23387704 .. 23392344

Gene Structure 4641 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1147000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3387 bp

ATGCCCACCTTCTTCATCTTCCTCCTGCTCTTCTTATCCTCTCCACTCACTGCATCTCCAAGGCCAAACTATAGAGTCCTACACCAGCCATTTCTGCCGCAGGAATCTTCTTCGCCGGCGCCCACTCAGCCACCATCTCCATCCCCGCCTTCCCCTCCTCCCAACCCAACTCCCCCGAAGTATCCATTTTCCAGTTCCAATTCTCCCCCTCCCAATTCCCCATTTTTCCCATCCAACCCTTCCCCTCCGCCTCCCCCTCCCGCCCCAGCCACCGCCTTCGCCTCATTCCCCGCCAACATCTCCTCCCTCATCGTCCCCCAGCCCACCAAAAACTCCAGCTCCCACAAGCTCCTCGGCCTCACTATCGCCGCCGTGCTCTCCGCCGCCGTCGTGGTGGCCGTAGCCGCATTCTTCCTCTACCGTCGCAGACGGCACAACAGCCGCAACTTCCCCGACGAGGATGAGGACGAGAAGTCGTATCTTAGATCCGAGCACAGCAACAGGCTGTTACAGGCGCCCAATCTCGGCCACTCCTACAGTGGGACCCACAAGCTCCGGACCGGCTCCTCCACCAGCTCCGAGTTCCTTTACCTGGGCACATTGGTCAATTCACGCTCACTTGAGGAGAGAGTCGACTCGGGCGGGAGTGTTGGTCGAAGCGCCGAATTAGAAACTCGGAAAGTCGACTCACCGGACCTCCAGCCGCTTCCGCCGCTTTCTCGGCAGATCTCGAGGCTGAGCCGTTGCAAGAATGCCGAACTGGGTTCGACTCAGGACCGAGACGATGAGGAAGAGGAAGAGGAAGAAGAGTTCTATTCCCCCAGAGGGTCTTCGGGCGATCGCGAGAGCTCCAACGAAAACGGATCCGGGTCCAGGAGAATGTTGGCGGCGGTAGCGGGTGGAGTATTTGACCGAAGAAGCAGCGAAACCACGTCGTGTTCTTGCTCGTCTTCCGAATTGGGTTCACCGCCTCGATCACACTCTATCAGTCTCTCGCCTCCGGTGACTTTGAGTCCCAACAGAAGACCAGAAGAGCCCAAACTGCCGGAGCCCTCAGCCACGCGTTATACGACGCCGTTTGGGGACGTCAATGTACGGTCTCCGTCGTTGACTCCACTATCTTCGCCGGAGAGAGCTGCTGACGAATACCCATCTGCATCGGACCGAAACGGAAGGTCTCCGTCGATGTCCTCGTTATCTTCGTCGCCGGAGAAAGGATTGGAGAAGACCCCAGATGCACCGCTGAAAGTTTCGGTAGTTTCGGATCAAAGTTCGCCGATTTCTTCACCGGAGAGAGGTTTCGGAAACAACCCACATGCATCATCGAAAGTTTCGGTAGTTACGGATCAAAGTTCGCTGATTTCGTCACCGGAGAGACATTTCGGAATCCATCCAGATGCATTATCGAAAGTTTCGGTGGTTTCGGGTCAGAGTTCTCCTATTTCTTCACCGGAATTAAATTTCGGAAACAACACAGATGGGTTAACGAAAGTTACGGCCTTTCCGGATCGGAATGATCAGTCCCCTTCCCCATTATCACATTGTTCATCGTCACCGGAGAGAGAATCAGACGGTTCTGATTCAAAAGCCAGGTCGTTTTCACCTTCTATGTCACCACTGAGAGGTTTGGAGAACTCAGCTGCCTCACCAAGAATTTCAAATGCTTCGGATCGAGCATTTATTCATTTGGATCCCAAAATGCAGTCTCTTTCATCATCTTCATCGTCTTCGTTGTCAAATTCGCCGGAGAGAGCGTTTAGTGTTGGTTTAAAGCAGCCTCTTTCGGTTCCTCCGCCGCCACCGCCAATGCCTCCTTCACTGAGACTCTGGGAGACTCCAAGTCCTAGAACACCAGTTGGCCAAGTGATCTCTAGGCCTCCAGCTCTCATACCTCCTTCAAGACCTTTTGTGATTCAAAACCCGGGAAAGGTCCCTGTTTCGCCGGTGGAGTTGCCACCAAGTTCTACGCCGTTGGAGCCGATTGAGGAGACTCCGAAACCCAAGTTGAAGCCATTGCATTGGGATAAAGTTAGAGCAAGCTCCGACCGCGAAATGGTGTGGGATCAGCTTAGATCAAGCTCTTTCAAATTGAATGAAGAAATGATAGAGACATTGTTTGTTGTAAAAACACCGAACCCGAATCCAAAAGAAACAGCAGTTCTTCCCTCACTGAACCAAGAGAACAGAGTGCTTGATCCTAAAAAGTCGCAAAATATTGCAATCTCGCTAAGGGCACTCAATGTGACCATAGAGGAAGTTTGCGAAGCTTTATTAGAAGGAAACTCGGATGCTCTTGGAACTGAATTACTTGAAAGTCTAATGAAGATGGCTCCAACCAAAGAAGAAGAACGTAAGCTGAAGGAATACAAAGACGACTCACCAGTTAAGCTTGGTCCTGCCGAGAGATTTTTGAAAGAGCTGCTTGACGTTCCTTTTGCATTTAGAAGGATTGATGCAATGCTTTACGTGACTAATTTTGAGTCTGAGATCGAGTACCTTAAAAAATCGTTTGAAACTCTGGAGGCTGCCTGTGAAGAATTGAGAAACAGCAGGATGTTCTTGAAGCTTCTGGAAGCTGTTCTAAAGACTGGAAACCGGATGAATGTTGGGACAAACCGTGGTGATGCCCATGCCTTCAAACTGGACACACTCCTCAAGCTGGTTGACGTCAAGGGGGCAGATGGGAAAACAACACTCCTGCATTTTGTCGTACAAGAAATCATAAGAACAGAAGGTGCTCGTCTCACCGGTGGCAATCAAACTTCAAACCCAACTTTGAGTGATGATGTCAAGTGCAGGAGGCTTGGCCTGCAAGTTGTTTCAGCTCTCAGTTCAGACCTCAGTAATGTGAAGAAAGCTGCTGCCATGGATTTTGATGTGCTTAGCAGTGATGTTTCCAAGCTTTCTAAAGGAATTAGCAACATCGCAGAGGTTGTGCAATTGAATGAGACAACAGTATCAGATGACAGCAGGCGGAAATTCTCAGAGTCAATGAACATGTTCATGAAATTGGCTGAAGAGGAGATTATAAGACTTCAAGCTCAAGAAAGTGTTGCACTGTCCCTGGTGAAGGAGATTACAGAGTACTTCCATGGAAACTCCGCTAGGGAAGAAGCTCACCCGTTTAGAATCTTCACGGTGGTCAGAGACTTTCTTACAGTTCTTGATCGGGTATGCAAGGAAGTTGGTATGATAAATGAGCGAACACAAGTTAGTACCGCCCACAAATTTCCAGTTCCGGTAAATCCAATGCTACCACAAATAGTTCCAGTAAATCCAATGCTGCCACAAGTAGTTCCAGTAAATCCAATGCCACCACAAGTACTTCCTGGAATTCATGGGTTAAGGCGGTACAGTTCGTCCGATGATGAGAGCACAGCTCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

1129

Amino Acids

123.06

Weight (kDa)

6.12

Isoelectric Point (pI)

74.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	663 - 1055	2.4e-121	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25500
fragaria_vesca	FvH4_2g17360 FvH4_2g17360 FvH4_2g17360
malus_domestica	MD05G1152500.v1.1 MD10G1147000.v1.1
prunus_persica	Prupe.8G185400_v2.0.a1
pyrus_communis	pycom05g14690 pycom10g12670
rosa_chinensis	RchiOBHm_Chr6g0281991
rosa_laevigata	RLG00000012921
rosa_multiflora	Rmu_sc0000569.1_g000001 Rmu_sc0001255.1_g000001 Rmu_sc0011823.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186650
rosa_rugosa	Rorug06G0144700
rosa_samantha	Rh4CG162200 Rh4CG270300 Rh6AG257900 Rh6BG260900 Rh6CG259800 Rh6DG252200 Rh7AG416400
rosa_wichuraiana	Rw6G022300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3029
AatII	GACGTC	2 cut(s)	1089, 2670
AccB1I	GGYRCC	2 cut(s)	118, 514
AccI	GTMKAC	2 cut(s)	633, 684
AccII	CGCG	3 cut(s)	843, 1063, 2049
AccIII	TCCGGA	2 cut(s)	555, 1504
AcoI	YGGCCR	3 cut(s)	396, 526, 1858
AcsI	RAATTY	6 cut(s)	1459, 1653, 1738, 2978, 3227, 3333
AcuI	CTGAAG	2 cut(s)	2378, 3036
AcyI	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
AdeI	CACNNNGTG	1 cut(s)	936
AfaI	GTAC	7 cut(s)	1095, 2495, 2712, 3086, 3217, 3324, 3353
AflIII	ACRYGT	2 cut(s)	1061, 2997
AgeI	ACCGGT	1 cut(s)	2747
AhdI	GACNNNNNGTC	3 cut(s)	472, 1011, 1839
AjiI	CACGTC	1 cut(s)	933
AjnI	CCWGG	5 cut(s)	591, 872, 1589, 3063, 3328
AjuI	GAANNNNNNNTTGG	2 cut(s)	1798, 1830
AloI	GAACNNNNNNTCC	2 cut(s)	794, 826
Alw21I	GWGCWC	5 cut(s)	378, 495, 851, 2740, 3377
Ama87I	CYCGRG	3 cut(s)	637, 730, 1921
Aor13HI	TCCGGA	2 cut(s)	555, 1504
AoxI	GGCC	8 cut(s)	62, 355, 396, 526, 1497, 1858, 1874, 2806
ApeKI	GCWGC	9 cut(s)	921, 1136, 1640, 1774, 2419, 2525, 2858, 2861, 3283
ApoI	RAATTY	6 cut(s)	1459, 1653, 1738, 2978, 3227, 3333
ArsI	GACNNNNNNTTYG	4 cut(s)	1577, 1609, 2417, 2449
AsiGI	ACCGGT	1 cut(s)	2747
AsiSI	GCGATCGC	1 cut(s)	841
Asp700I	GAANNNNTTC	2 cut(s)	2577, 2999
AspLEI	GCGC	3 cut(s)	121, 517, 662
AspS9I	GGNCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
AsuC2I	CCSGG	3 cut(s)	868, 1922, 1923
AvaI	CYCGRG	3 cut(s)	637, 730, 1921
AvaII	GGWCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
BaeGI	GKGCMC	2 cut(s)	599, 2236
BaeI	ACNNNNGTAYC	2 cut(s)	2940, 2973
BalI	TGGCCA	1 cut(s)	1860
BamHI	GGATCC	2 cut(s)	863, 1690
BanI	GGYRCC	2 cut(s)	118, 514
BanII	GRGCYC	3 cut(s)	851, 1038, 1053
BbsI	GAAGAC	5 cut(s)	822, 942, 1030, 1232, 1719
Bbv12I	GWGCWC	5 cut(s)	378, 495, 851, 2740, 3377
BbvCI	CCTCAGC	1 cut(s)	1054
BbvI	GCAGC	9 cut(s)	933, 1123, 1627, 1786, 2406, 2512, 2845, 2848, 3270
BccI	CCATC	8 cut(s)	142, 148, 238, 1159, 1398, 1472, 2320, 2675
BceAI	ACGGC	8 cut(s)	356, 371, 383, 446, 726, 1060, 1512, 1954
BciT130I	CCWGG	5 cut(s)	593, 874, 1591, 3065, 3330
BciVI	GTATCC	1 cut(s)	192
BclI	TGATCA	1 cut(s)	1516
BcnI	CCSGG	3 cut(s)	868, 1922, 1923
BfaI	CTAG	3 cut(s)	1845, 1872, 3105
BfmI	CTRYAG	2 cut(s)	70, 535
BfoI	RGCGCY	3 cut(s)	122, 518, 663
BfuI	GTATCC	1 cut(s)	192
BglII	AGATCT	1 cut(s)	726
BlpI	GCTNAGC	2 cut(s)	737, 2882
BmcAI	AGTACT	2 cut(s)	3086, 3324
Bme1390I	CCNGG	8 cut(s)	593, 868, 874, 1591, 1922, 1923, 3065, 3330
Bme18I	GGWCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
BmeRI	GACNNNNNGTC	3 cut(s)	472, 1011, 1839
BmeT110I	CYCGRG	3 cut(s)	637, 730, 1921
BmgBI	CACGTC	1 cut(s)	933
BmgT120I	GGNCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
BmrFI	CCNGG	8 cut(s)	593, 868, 874, 1591, 1922, 1923, 3065, 3330
BmrI	ACTGGG	1 cut(s)	770
BmsI	GCATC	8 cut(s)	62, 1166, 1225, 1328, 1387, 2278, 2443, 2614
BmuI	ACTGGG	1 cut(s)	770
BpiI	GAAGAC	5 cut(s)	822, 942, 1030, 1232, 1719
BplI	GAGNNNNNCTC	1 cut(s)	3364
BpmI	CTGGAG	4 cut(s)	319, 683, 1863, 2540
Bpu10I	CCTNAGC	2 cut(s)	1054, 2228
Bpu1102I	GCTNAGC	2 cut(s)	737, 2882
BpuEI	CTTGAG	3 cut(s)	641, 2639, 3027
BpuMI	CCSGG	3 cut(s)	868, 1922, 1923
BsaAI	YACGTR	1 cut(s)	2467
BsaHI	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
BsaI	GGTCTC	2 cut(s)	1104, 1182
BsaJI	CCNNGG	8 cut(s)	59, 352, 592, 1921, 2617, 2865, 3063, 3091
Bse118I	RCCGGY	4 cut(s)	115, 560, 1942, 2747
Bse1I	ACTGG	9 cut(s)	192, 765, 1853, 2381, 2593, 2646, 3233, 3269, 3299
Bse3DI	GCAATG	2 cut(s)	2012, 2463
BseAI	TCCGGA	2 cut(s)	555, 1504
BseBI	CCWGG	5 cut(s)	593, 874, 1591, 3065, 3330
BseDI	CCNNGG	8 cut(s)	59, 352, 592, 1921, 2617, 2865, 3063, 3091
BseGI	GGATG	7 cut(s)	140, 230, 466, 1390, 2293, 2559, 2604
BseMI	GCAATG	2 cut(s)	2012, 2463
BseNI	ACTGG	9 cut(s)	192, 765, 1853, 2381, 2593, 2646, 3233, 3269, 3299
BseRI	GAGGAG	8 cut(s)	147, 292, 341, 556, 638, 2000, 2642, 3035
BseSI	GKGCMC	2 cut(s)	599, 2236
BseXI	GCAGC	9 cut(s)	933, 1123, 1627, 1786, 2406, 2512, 2845, 2848, 3270
BseYI	CCCAGC	2 cut(s)	265, 318
BsgI	GTGCAG	1 cut(s)	2815
Bsh1236I	CGCG	3 cut(s)	843, 1063, 2049
Bsh1285I	CGRYCG	2 cut(s)	841, 2047
BshFI	GGCC	8 cut(s)	64, 357, 398, 528, 1499, 1860, 1876, 2808
BshNI	GGYRCC	2 cut(s)	118, 514
BshTI	ACCGGT	1 cut(s)	2747
BsiEI	CGRYCG	2 cut(s)	841, 2047
BsiHKAI	GWGCWC	5 cut(s)	378, 495, 851, 2740, 3377
BsiHKCI	CYCGRG	3 cut(s)	637, 730, 1921
BslFI	GGGAC	8 cut(s)	299, 556, 999, 1097, 1507, 1916, 2623, 3046
BsmBI	CGTCTC	2 cut(s)	774, 2747
BsmFI	GGGAC	8 cut(s)	299, 556, 999, 1097, 1507, 1916, 2623, 3046
BsmI	GAATGC	2 cut(s)	407, 757
BsnI	GGCC	8 cut(s)	64, 357, 398, 528, 1499, 1860, 1876, 2808
Bso31I	GGTCTC	2 cut(s)	1104, 1182
BsoBI	CYCGRG	3 cut(s)	637, 730, 1921
Bsp1286I	GDGCHC	9 cut(s)	378, 495, 599, 851, 1038, 1053, 2236, 2740, 3377
Bsp13I	TCCGGA	2 cut(s)	555, 1504
Bsp1720I	GCTNAGC	2 cut(s)	737, 2882
Bsp19I	CCATGG	2 cut(s)	2865, 3091
Bsp68I	TCGCGA	1 cut(s)	843
BspANI	GGCC	8 cut(s)	64, 357, 398, 528, 1499, 1860, 1876, 2808
BspEI	TCCGGA	2 cut(s)	555, 1504
BspFNI	CGCG	3 cut(s)	843, 1063, 2049
BspHI	TCATGA	1 cut(s)	3003
BspQI	GCTCTTC	2 cut(s)	35, 1026
BspT107I	GGYRCC	2 cut(s)	118, 514
BspTNI	GGTCTC	2 cut(s)	1104, 1182
BsrDI	GCAATG	2 cut(s)	2012, 2463
BsrFI	RCCGGY	4 cut(s)	115, 560, 1942, 2747
BsrI	ACTGG	9 cut(s)	192, 765, 1853, 2381, 2593, 2646, 3233, 3269, 3299
BssAI	RCCGGY	4 cut(s)	115, 560, 1942, 2747
BssECI	CCNNGG	8 cut(s)	59, 352, 592, 1921, 2617, 2865, 3063, 3091
BssNI	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
BssT1I	CCWWGG	3 cut(s)	59, 2865, 3091
Bst2UI	CCWGG	5 cut(s)	593, 874, 1591, 3065, 3330
Bst6I	CTCTTC	6 cut(s)	35, 786, 792, 801, 1026, 3012
BstACI	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
BstBAI	YACGTR	1 cut(s)	2467
BstC8I	GCNNGC	4 cut(s)	117, 2038, 2810, 2972
BstDSI	CCRYGG	3 cut(s)	2617, 2865, 3091
BstENI	CCTNNNNNAGG	1 cut(s)	3068
BstF5I	GGATG	7 cut(s)	140, 230, 466, 1390, 2293, 2559, 2604
BstFNI	CGCG	3 cut(s)	843, 1063, 2049
BstH2I	RGCGCY	3 cut(s)	122, 518, 663
BstHHI	GCGC	3 cut(s)	121, 517, 662
BstMCI	CGRYCG	2 cut(s)	841, 2047
BstNI	CCWGG	5 cut(s)	593, 874, 1591, 3065, 3330
BstNSI	RCATGY	2 cut(s)	1319, 3001
BstSCI	CCNGG	8 cut(s)	591, 866, 872, 1589, 1920, 1921, 3063, 3328
BstSFI	CTRYAG	2 cut(s)	70, 535
BstSLI	GKGCMC	2 cut(s)	599, 2236
BstUI	CGCG	3 cut(s)	843, 1063, 2049
BstV1I	GCAGC	9 cut(s)	933, 1123, 1627, 1786, 2406, 2512, 2845, 2848, 3270
BstV2I	GAAGAC	5 cut(s)	822, 942, 1030, 1232, 1719
BstX2I	RGATCY	4 cut(s)	485, 726, 863, 1690
BstXI	CCANNNNNNTGG	1 cut(s)	2019
BstYI	RGATCY	4 cut(s)	485, 726, 863, 1690
BsuI	GTATCC	1 cut(s)	192
BsuRI	GGCC	8 cut(s)	64, 357, 398, 528, 1499, 1860, 1876, 2808
BtgI	CCRYGG	3 cut(s)	2617, 2865, 3091
BtgZI	GCGATG	1 cut(s)	2908
BtrI	CACGTC	1 cut(s)	933
BtsCI	GGATG	7 cut(s)	140, 230, 466, 1390, 2293, 2559, 2604
BtsI	GCAGTG	2 cut(s)	48, 2893
BtsIMutI	CAGTG	7 cut(s)	48, 544, 1616, 1817, 2168, 2893, 3056
BtuMI	TCGCGA	1 cut(s)	843
Cac8I	GCNNGC	4 cut(s)	117, 2038, 2810, 2972
CciI	TCATGA	1 cut(s)	3003
CfoI	GCGC	3 cut(s)	121, 517, 662
Cfr10I	RCCGGY	4 cut(s)	115, 560, 1942, 2747
Cfr13I	GGNCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
Cfr9I	CCCGGG	1 cut(s)	1921
CpoI	CGGWCCG	2 cut(s)	558, 1162
CseI	GACGC	1 cut(s)	1081
Csp6I	GTAC	7 cut(s)	1094, 2494, 2711, 3085, 3216, 3323, 3352
CspAI	ACCGGT	1 cut(s)	2747
CspI	CGGWCCG	2 cut(s)	558, 1162
CviAII	CATG	7 cut(s)	1316, 2630, 2866, 2998, 3004, 3092, 3338
CviQI	GTAC	7 cut(s)	1094, 2494, 2711, 3085, 3216, 3323, 3352
DinI	GGCGCC	2 cut(s)	120, 516
DraI	TTTAAA	1 cut(s)	1770
DraIII	CACNNNGTG	1 cut(s)	936
DriI	GACNNNNNGTC	3 cut(s)	472, 1011, 1839
EaeI	YGGCCR	3 cut(s)	396, 526, 1858
Eam1104I	CTCTTC	6 cut(s)	35, 786, 792, 801, 1026, 3012
Eam1105I	GACNNNNNGTC	3 cut(s)	472, 1011, 1839
EarI	CTCTTC	6 cut(s)	35, 786, 792, 801, 1026, 3012
EciI	GGCGGA	5 cut(s)	237, 370, 699, 1782, 2989
Ecl136II	GAGCTC	1 cut(s)	849
Eco130I	CCWWGG	3 cut(s)	59, 2865, 3091
Eco147I	AGGCCT	1 cut(s)	1876
Eco24I	GRGCYC	3 cut(s)	851, 1038, 1053
Eco31I	GGTCTC	2 cut(s)	1104, 1182
Eco47I	GGWCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
Eco53kI	GAGCTC	1 cut(s)	849
Eco57I	CTGAAG	2 cut(s)	2378, 3036
Eco88I	CYCGRG	3 cut(s)	637, 730, 1921
EcoICRI	GAGCTC	1 cut(s)	849
EcoNI	CCTNNNNNAGG	1 cut(s)	3068
EcoO109I	RGGNCCY	2 cut(s)	543, 1930
EcoRI	GAATTC	1 cut(s)	3333
EcoRII	CCWGG	5 cut(s)	591, 872, 1589, 3063, 3328
EcoT14I	CCWWGG	3 cut(s)	59, 2865, 3091
EcoT22I	ATGCAT	2 cut(s)	1321, 1402
EcoT38I	GRGCYC	3 cut(s)	851, 1038, 1053
EgeI	GGCGCC	2 cut(s)	120, 516
EheI	GGCGCC	2 cut(s)	120, 516
ErhI	CCWWGG	3 cut(s)	59, 2865, 3091
Esp3I	CGTCTC	2 cut(s)	774, 2747
FaeI	CATG	7 cut(s)	1319, 2633, 2869, 3001, 3007, 3095, 3341
FalI	AAGNNNNNCTT	2 cut(s)	2406, 2438
FaqI	GGGAC	8 cut(s)	299, 556, 999, 1097, 1507, 1916, 2623, 3046
FatI	CATG	7 cut(s)	1315, 2629, 2865, 2997, 3003, 3091, 3337
FauI	CCCGC	5 cut(s)	153, 268, 298, 635, 889
FbaI	TGATCA	1 cut(s)	1516
FblI	GTMKAC	2 cut(s)	633, 684
FokI	GGATG	7 cut(s)	127, 217, 473, 1377, 2300, 2566, 2611
FriOI	GRGCYC	3 cut(s)	851, 1038, 1053
FspBI	CTAG	3 cut(s)	1845, 1872, 3105
GlaI	GCGC	3 cut(s)	120, 516, 661
GsaI	CCCAGC	2 cut(s)	269, 322
GsuI	CTGGAG	4 cut(s)	319, 683, 1863, 2540
HaeII	RGCGCY	3 cut(s)	122, 518, 663
HaeIII	GGCC	8 cut(s)	64, 357, 398, 528, 1499, 1860, 1876, 2808
HgaI	GACGC	1 cut(s)	1081
HhaI	GCGC	3 cut(s)	121, 517, 662
Hin1I	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
Hin1II	CATG	7 cut(s)	1319, 2633, 2869, 3001, 3007, 3095, 3341
Hin6I	GCGC	3 cut(s)	119, 515, 660
HinP1I	GCGC	3 cut(s)	119, 515, 660
HincII	GTYRAC	5 cut(s)	634, 685, 1110, 1485, 2665
HindII	GTYRAC	5 cut(s)	634, 685, 1110, 1485, 2665
HindIII	AAGCTT	4 cut(s)	2265, 2387, 2564, 2900
HpaI	GTTAAC	1 cut(s)	1485
Hpy166II	GTNNAC	6 cut(s)	634, 685, 965, 1110, 1485, 2665
Hpy8I	GTNNAC	6 cut(s)	634, 685, 965, 1110, 1485, 2665
Hpy99I	CGWCG	8 cut(s)	392, 426, 458, 937, 1075, 1108, 1186, 1207
HpyCH4IV	ACGT	6 cut(s)	932, 1086, 2350, 2427, 2466, 2667
HpySE526I	ACGT	6 cut(s)	932, 1086, 2350, 2427, 2466, 2667
Hsp92I	GRCGYC	5 cut(s)	119, 515, 1073, 1086, 2667
Hsp92II	CATG	7 cut(s)	1319, 2633, 2869, 3001, 3007, 3095, 3341
HspAI	GCGC	3 cut(s)	119, 515, 660
KasI	GGCGCC	2 cut(s)	118, 514
KflI	GGGWCCC	1 cut(s)	543
Kpn2I	TCCGGA	2 cut(s)	555, 1504
KroI	GCCGGC	1 cut(s)	115
KroNI	GCCGGC	1 cut(s)	117
Ksp22I	TGATCA	1 cut(s)	1516
KspAI	GTTAAC	1 cut(s)	1485
LguI	GCTCTTC	2 cut(s)	35, 1026
Lsp1109I	GCAGC	9 cut(s)	933, 1123, 1627, 1786, 2406, 2512, 2845, 2848, 3270
LweI	GCATC	8 cut(s)	62, 1166, 1225, 1328, 1387, 2278, 2443, 2614
MaeI	CTAG	3 cut(s)	1845, 1872, 3105
MaeII	ACGT	6 cut(s)	932, 1086, 2350, 2427, 2466, 2667
MaeIII	GTNAC	9 cut(s)	507, 1003, 1339, 1365, 1492, 1551, 1611, 2242, 2467
MfeI	CAATTG	1 cut(s)	2939
MflI	RGATCY	4 cut(s)	485, 726, 863, 1690
MhlI	GDGCHC	9 cut(s)	378, 495, 599, 851, 1038, 1053, 2236, 2740, 3377
MlsI	TGGCCA	1 cut(s)	1860
MluI	ACGCGT	1 cut(s)	1061
MluNI	TGGCCA	1 cut(s)	1860
Mly113I	GGCGCC	2 cut(s)	119, 515
MmeI	TCCRAC	5 cut(s)	258, 876, 1953, 2067, 2357
Mox20I	TGGCCA	1 cut(s)	1860
Mph1103I	ATGCAT	2 cut(s)	1321, 1402
MreI	CGCCGGCG	1 cut(s)	115
MroI	TCCGGA	2 cut(s)	555, 1504
MroNI	GCCGGC	1 cut(s)	115
MroXI	GAANNNNTTC	2 cut(s)	2577, 2999
MscI	TGGCCA	1 cut(s)	1860
MseI	TTAA	6 cut(s)	1457, 1484, 1769, 2385, 2499, 3344
MslI	CAYNNNNRTG	2 cut(s)	1395, 3002
Msp20I	TGGCCA	1 cut(s)	1860
MspA1I	CMGCKG	2 cut(s)	1243, 1640
MspR9I	CCNGG	8 cut(s)	593, 868, 874, 1591, 1922, 1923, 3065, 3330
MunI	CAATTG	1 cut(s)	2939
Mva1269I	GAATGC	2 cut(s)	407, 757
MvaI	CCWGG	5 cut(s)	593, 874, 1591, 3065, 3330
MvnI	CGCG	3 cut(s)	843, 1063, 2049
NaeI	GCCGGC	1 cut(s)	117
NarI	GGCGCC	2 cut(s)	119, 515
NciI	CCSGG	3 cut(s)	868, 1922, 1923
NcoI	CCATGG	2 cut(s)	2865, 3091
NgoMIV	GCCGGC	1 cut(s)	115
NlaIII	CATG	7 cut(s)	1319, 2633, 2869, 3001, 3007, 3095, 3341
NmeAIII	GCCGAG	4 cut(s)	333, 504, 700, 2425
NmuCI	GTSAC	6 cut(s)	1003, 1365, 1551, 1611, 2242, 2467
NruI	TCGCGA	1 cut(s)	843
NsiI	ATGCAT	2 cut(s)	1321, 1402
NspI	RCATGY	2 cut(s)	1319, 3001
PaeR7I	CTCGAG	1 cut(s)	730
PagI	TCATGA	1 cut(s)	3003
PceI	AGGCCT	1 cut(s)	1876
PciI	ACATGT	1 cut(s)	2997
PciSI	GCTCTTC	2 cut(s)	35, 1026
PcsI	WCGNNNNNNNCGW	5 cut(s)	396, 1067, 1190, 1199, 1361
PctI	GAATGC	2 cut(s)	407, 757
PdiI	GCCGGC	1 cut(s)	117
PdmI	GAANNNNTTC	2 cut(s)	2577, 2999
PfeI	GAWTC	7 cut(s)	104, 1386, 1565, 1580, 1912, 2140, 3129
PfoI	TCCNGGA	2 cut(s)	872, 3328
PinAI	ACCGGT	1 cut(s)	2747
Ple19I	CGATCG	1 cut(s)	841
PluTI	GGCGCC	2 cut(s)	122, 518
Ppu21I	YACGTR	1 cut(s)	2467
PpuMI	RGGWCCY	2 cut(s)	543, 1930
PscI	ACATGT	1 cut(s)	2997
PsiI	TTATAA	1 cut(s)	3029
Psp124BI	GAGCTC	1 cut(s)	851
Psp5II	RGGWCCY	2 cut(s)	543, 1930
Psp6I	CCWGG	5 cut(s)	591, 872, 1589, 3063, 3328
PspFI	CCCAGC	2 cut(s)	265, 318
PspGI	CCWGG	5 cut(s)	591, 872, 1589, 3063, 3328
PspPI	GGNCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
PspPPI	RGGWCCY	2 cut(s)	543, 1930
PsuI	RGATCY	4 cut(s)	485, 726, 863, 1690
PvuI	CGATCG	1 cut(s)	841
PvuII	CAGCTG	1 cut(s)	1640
RgaI	GCGATCGC	1 cut(s)	841
RruI	TCGCGA	1 cut(s)	843
RsaI	GTAC	7 cut(s)	1095, 2495, 2712, 3086, 3217, 3324, 3353
RsaNI	GTAC	7 cut(s)	1094, 2494, 2711, 3085, 3216, 3323, 3352
RseI	CAYNNNNRTG	2 cut(s)	1395, 3002
Rsr2I	CGGWCCG	2 cut(s)	558, 1162
RsrII	CGGWCCG	2 cut(s)	558, 1162
SacI	GAGCTC	1 cut(s)	851
SalI	GTCGAC	2 cut(s)	632, 683
SapI	GCTCTTC	2 cut(s)	35, 1026
SaqAI	TTAA	6 cut(s)	1457, 1484, 1769, 2385, 2499, 3344
Sau96I	GGNCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
ScaI	AGTACT	2 cut(s)	3086, 3324
ScrFI	CCNGG	8 cut(s)	593, 868, 874, 1591, 1922, 1923, 3065, 3330
SduI	GDGCHC	9 cut(s)	378, 495, 599, 851, 1038, 1053, 2236, 2740, 3377
SfaAI	GCGATCGC	1 cut(s)	841
SfaNI	GCATC	8 cut(s)	62, 1166, 1225, 1328, 1387, 2278, 2443, 2614
SfcI	CTRYAG	2 cut(s)	70, 535
SfoI	GGCGCC	2 cut(s)	120, 516
Sfr274I	CTCGAG	1 cut(s)	730
SgfI	GCGATCGC	1 cut(s)	841
SgrAI	CRCCGGYG	3 cut(s)	115, 1942, 2747
SinI	GGWCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
SlaI	CTCGAG	1 cut(s)	730
SmaI	CCCGGG	1 cut(s)	1923
SmiMI	CAYNNNNRTG	2 cut(s)	1395, 3002
SmlI	CTYRAG	4 cut(s)	620, 730, 2654, 3042
SmoI	CTYRAG	4 cut(s)	620, 730, 2654, 3042
SseBI	AGGCCT	1 cut(s)	1876
SspDI	GGCGCC	2 cut(s)	118, 514
SspI	AATATT	1 cut(s)	2215
SspMI	CTAG	3 cut(s)	1845, 1872, 3105
SstI	GAGCTC	1 cut(s)	851
StuI	AGGCCT	1 cut(s)	1876
StyD4I	CCNGG	8 cut(s)	591, 866, 872, 1589, 1920, 1921, 3063, 3328
StyI	CCWWGG	3 cut(s)	59, 2865, 3091
TaiI	ACGT	6 cut(s)	935, 1089, 2353, 2430, 2469, 2670
TaqII	GACCGA	3 cut(s)	792, 927, 1179
TatI	WGTACW	2 cut(s)	3084, 3322
TauI	GCSGC	9 cut(s)	100, 371, 386, 407, 444, 706, 715, 890, 1798
TfiI	GAWTC	7 cut(s)	104, 1386, 1565, 1580, 1912, 2140, 3129
Tru1I	TTAA	6 cut(s)	1457, 1484, 1769, 2385, 2499, 3344
Tru9I	TTAA	6 cut(s)	1457, 1484, 1769, 2385, 2499, 3344
TscAI	CASTG	7 cut(s)	55, 544, 1623, 1824, 2175, 2893, 3063
TseFI	GTSAC	6 cut(s)	1003, 1365, 1551, 1611, 2242, 2467
TseI	GCWGC	9 cut(s)	921, 1136, 1640, 1774, 2419, 2525, 2858, 2861, 3283
Tsp45I	GTSAC	6 cut(s)	1003, 1365, 1551, 1611, 2242, 2467
TspGWI	ACGGA	6 cut(s)	876, 1092, 1170, 1185, 1358, 3372
TspMI	CCCGGG	1 cut(s)	1921
TspRI	CASTG	7 cut(s)	55, 544, 1623, 1824, 2175, 2893, 3063
VpaK11BI	GGWCC	8 cut(s)	543, 558, 694, 775, 870, 1162, 1930, 2393
XagI	CCTNNNNNAGG	1 cut(s)	3068
XapI	RAATTY	6 cut(s)	1459, 1653, 1738, 2978, 3227, 3333
XceI	RCATGY	2 cut(s)	1319, 3001
XcmI	CCANNNNNNNNNTGG	2 cut(s)	536, 880
XhoI	CTCGAG	1 cut(s)	730
XmaI	CCCGGG	1 cut(s)	1921
XmiI	GTMKAC	2 cut(s)	633, 684
XmnI	GAANNNNTTC	2 cut(s)	2577, 2999
XspI	CTAG	3 cut(s)	1845, 1872, 3105
ZraI	GACGTC	2 cut(s)	1087, 2668
ZrmI	AGTACT	2 cut(s)	3086, 3324
Zsp2I	ATGCAT	2 cut(s)	1321, 1402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.