MD05G1163300.v1.1

dCTP pyrophosphatase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003799
Physical Location & Seq
Reverse (-)
29213006 .. 29214728
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1163300.v1.1.491

Sequence Viewer

Length: 408 bp
ATGACTGGTTTTGCAGAAGGAGAAAATGTCACCCTTGATCTTCTCAAGAACAAAATGGCTGAGTTTGCCAAAGCGAGAAACTGGGATCAGTTCCACAGCCCCAGAAACCTTCTCCTAGCTCTGGTGTGCGAAGTGGGAGAGCTGTCTGAGATATTTCAATGGAAAGGGGAGGTTCCAAAGGGGCTGCCAGATTGGAAAGAGGAGGAAAAGCAGCACCTGGGGGAAGAGCTTTCTGATGTGCTGCTTTATCTGGTCAGGCTCTCTGACATTTGTGGTGTTGATCTTGGCAAAGCTGCCCTGCGCAAGGTCGAACTCAATTCCATTAAGTATCCTGTTTCACAGAAACAGAGCCAAACAACCACAACCACCGCCACTGCCACCAATGAGGAAATTGCACAGATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
MazG MazG-like

Protein Analysis

136

Amino Acids

15.12

Weight (kDa)

4.97

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MazG-like PF12643 43 - 117 2.9e-06 MazG-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 302
AciI CCGC 1 cut(s) 369
AclWI GGATC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 121, 304
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 4 cut(s) 119, 142, 229, 293
AluI AGCT 4 cut(s) 119, 142, 229, 293
AlwI GGATC 1 cut(s) 93
AlwNI CAGNNNCTG 1 cut(s) 217
ApeKI GCWGC 4 cut(s) 184, 211, 241, 293
AspLEI GCGC 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 22
BbvI GCAGC 4 cut(s) 171, 223, 228, 280
BccI CCATC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 218
BciVI GTATCC 1 cut(s) 339
BfaI CTAG 1 cut(s) 116
BfuI GTATCC 1 cut(s) 339
BisI GCNGC 4 cut(s) 185, 212, 242, 294
BlsI GCNGC 4 cut(s) 186, 213, 243, 295
Bme1390I CCNGG 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 218
BmrI ACTGGG 1 cut(s) 91
BmuI ACTGGG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 304
Bse1I ACTGG 2 cut(s) 10, 86
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 121, 304
BseMII CTCAG 2 cut(s) 51, 138
BseNI ACTGG 2 cut(s) 10, 86
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 4 cut(s) 171, 223, 228, 280
BslI CCNNNNNNNGG 2 cut(s) 121, 304
Bsp143I GATC 3 cut(s) 37, 85, 280
BspACI CCGC 1 cut(s) 369
BspCNI CTCAG 2 cut(s) 52, 139
BspLI GGNNCC 1 cut(s) 174
BspPI GGATC 1 cut(s) 93
BspQI GCTCTTC 1 cut(s) 219
BsrI ACTGG 2 cut(s) 10, 86
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 3 cut(s) 37, 85, 280
Bst2UI CCWGG 1 cut(s) 218
Bst6I CTCTTC 1 cut(s) 219
BstDEI CTNAG 2 cut(s) 60, 147
BstENI CCTNNNNNAGG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 3 cut(s) 40, 88, 283
BstMBI GATC 3 cut(s) 37, 85, 280
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 216
BstV1I GCAGC 4 cut(s) 171, 223, 228, 280
BsuI GTATCC 1 cut(s) 339
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 303
CviJI RGCY 9 cut(s) 59, 99, 119, 142, 184, 229, 259, 293, 351
CviKI_1 RGCY 9 cut(s) 59, 99, 119, 142, 184, 229, 259, 293, 351
DdeI CTNAG 2 cut(s) 60, 147
DpnI GATC 3 cut(s) 39, 87, 282
DpnII GATC 3 cut(s) 37, 85, 280
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 185, 212, 242, 294
Fsp4HI GCNGC 4 cut(s) 185, 212, 242, 294
FspBI CTAG 1 cut(s) 116
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 1 cut(s) 302
GluI GCNGC 4 cut(s) 185, 212, 242, 294
HhaI GCGC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HphI GGTGA 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 148, 235, 265
Hpy188III TCNNGA 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 11, 119
HpyCH4V TGCA 2 cut(s) 14, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 2 cut(s) 60, 147
HspAI GCGC 1 cut(s) 301
Kzo9I GATC 3 cut(s) 37, 85, 280
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 4 cut(s) 171, 223, 228, 280
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 39, 87, 282
MboI GATC 3 cut(s) 37, 85, 280
MboII GAAGA 2 cut(s) 32, 236
MluCI AATT 2 cut(s) 316, 390
MnlI CCTC 4 cut(s) 163, 193, 196, 379
MseI TTAA 2 cut(s) 324, 406
MspR9I CCNGG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 3 cut(s) 37, 85, 280
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 302
PciSI GCTCTTC 1 cut(s) 219
PkrI GCNGC 4 cut(s) 186, 213, 243, 295
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 324, 406
SatI GCNGC 4 cut(s) 185, 212, 242, 294
Sau3AI GATC 3 cut(s) 37, 85, 280
ScrFI CCNGG 1 cut(s) 218
SetI ASST 8 cut(s) 111, 121, 144, 174, 219, 231, 295, 309
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 316, 390
SsiI CCGC 1 cut(s) 369
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 216
TaqI TCGA 1 cut(s) 309
TasI AATT 2 cut(s) 316, 390
Tru1I TTAA 2 cut(s) 324, 406
Tru9I TTAA 2 cut(s) 324, 406
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 4 cut(s) 184, 211, 241, 293
Tsp45I GTSAC 1 cut(s) 28
TspRI CASTG 1 cut(s) 379
XagI CCTNNNNNAGG 1 cut(s) 302
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.