Rroxscaffold_7G00185700

dCTP pyrophosphatase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003799
Physical Location & Seq
Forward (+)
25114442 .. 25116226
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185700.1

Sequence Viewer

Length: 444 bp
ATGACTGGGTTTTCAGAATCCGAAAGCGTCACTCTTGACCTACTCAAGAAGAAGATGGCGGAGTTTTCTAGAGAGAGAAACTGGGATCAGTTCCACAGCCCCAGAAACTTGCTCTTGGCTCTGGTGGCTGAAGTGGGAGAGCTTTCTGAGATATTTCAATGGAAAGGAGAAGTTCCAAAGGGCCTTCCTGATTGGAAAGAAGAGGAAAAGCAACACCTGGGTGAAGAGCTCTCTGATGTCCTGCTCTATCTGGTCAGGCTCTCTGACATATGTGGTGTTGATCTTGGCAAAGCTGCTCTGCGCAAAGTTGAACTCAATGCCATTAAGTACCCAGTTTCACAGAAACAGAACCAGAACCAGACCAATGGCACCACCAACCCCCACACAACCACCGCCATTAACACCAGCAATGACAATAACAACACAGAAGAGATCACTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
MazG MazG-like

Protein Analysis

147

Amino Acids

16.57

Weight (kDa)

5.0

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MazG-like PF12643 43 - 119 1.1e-06 MazG-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 368
AciI CCGC 2 cut(s) 59, 393
AclWI GGATC 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 1 cut(s) 329
AgsI TTSAA 2 cut(s) 158, 311
AjnI CCWGG 1 cut(s) 216
AleI CACNNNNGTG 1 cut(s) 219
AluBI AGCT 3 cut(s) 142, 229, 293
AluI AGCT 3 cut(s) 142, 229, 293
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 93
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 1 cut(s) 293
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 233
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 218
BfaI CTAG 1 cut(s) 69
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
Bme1390I CCNGG 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 1 cut(s) 218
BmrI ACTGGG 3 cut(s) 15, 91, 326
BmuI ACTGGG 3 cut(s) 15, 91, 326
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 217
Bse1I ACTGG 4 cut(s) 10, 86, 332, 442
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 4 cut(s) 10, 86, 332, 442
BseXI GCAGC 1 cut(s) 280
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 368
BsiHKAI GWGCWC 1 cut(s) 231
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 3 cut(s) 85, 280, 432
BspACI CCGC 2 cut(s) 59, 393
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 370
BspPI GGATC 1 cut(s) 93
BspQI GCTCTTC 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 4 cut(s) 10, 86, 332, 442
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 3 cut(s) 85, 280, 432
Bst2UI CCWGG 1 cut(s) 218
Bst6I CTCTTC 3 cut(s) 195, 219, 423
BstDEI CTNAG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 3 cut(s) 88, 283, 435
BstMBI GATC 3 cut(s) 85, 280, 432
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 280
BstXI CCANNNNNNTGG 1 cut(s) 365
BsuRI GGCC 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 435
CfoI GCGC 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 181
CseI GACGC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 328
CviJI RGCY 8 cut(s) 99, 119, 128, 142, 183, 229, 259, 293
CviKI_1 RGCY 8 cut(s) 99, 119, 128, 142, 183, 229, 259, 293
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 1 cut(s) 147
DpnI GATC 3 cut(s) 87, 282, 434
DpnII GATC 3 cut(s) 85, 280, 432
Eam1104I CTCTTC 3 cut(s) 195, 219, 423
EarI CTCTTC 3 cut(s) 195, 219, 423
EciI GGCGGA 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 231
FaiI YATR 2 cut(s) 269, 271
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 1 cut(s) 294
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 1 cut(s) 69
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 1 cut(s) 302
GluI GCNGC 1 cut(s) 294
HaeIII GGCC 1 cut(s) 183
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HinfI GANTC 1 cut(s) 17
HphI GGTGA 1 cut(s) 233
Hpy188I TCNGA 5 cut(s) 16, 22, 148, 235, 265
Hpy188III TCNNGA 4 cut(s) 35, 46, 69, 188
HpyAV CCTTC 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 1 cut(s) 147
HspAI GCGC 1 cut(s) 301
Kzo9I GATC 3 cut(s) 85, 280, 432
LguI GCTCTTC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 280
MaeI CTAG 1 cut(s) 69
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 87, 282, 434
MboI GATC 3 cut(s) 85, 280, 432
MboII GAAGA 5 cut(s) 61, 64, 212, 236, 440
MhlI GDGCHC 1 cut(s) 231
MnlI CCTC 1 cut(s) 196
MseI TTAA 2 cut(s) 324, 399
MslI CAYNNNNRTG 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 85, 280, 432
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 1 cut(s) 181
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 219
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 324, 399
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 3 cut(s) 85, 280, 432
Sau96I GGNCC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 1 cut(s) 231
SetI ASST 5 cut(s) 42, 144, 219, 231, 295
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SsiI CCGC 2 cut(s) 59, 393
SspMI CTAG 1 cut(s) 69
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 216
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 2 cut(s) 324, 399
Tru9I TTAA 2 cut(s) 324, 399
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 28
TspRI CASTG 1 cut(s) 442
XbaI TCTAGA 1 cut(s) 68
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.