MD05G1170800.v1.1

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016401
Physical Location & Seq
Forward (+)
30031296 .. 30035573
4278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1170800.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGTGAGCTTAAGAAAGCGCAAACGCATGAGACTGTTCTCCGGGGAAAGTAATGCTTTAGTTCCACTTCCCAGTTTTTGGGACAAAGAAATTTCTCATCAAGATCCAAACCGCATGCCTTCAAATGATGTCATTCAGCCAGAGAGGATGAAAGAAACCAAGAAACCGAGGATAAAGACTTTTGCTAAGAAAAGCCCAACAAAAAAGAAGAAGGATTATCTGCAATATAGATGCAACATGATGGGCTTTGCAGATGCCATGCAATTGTACAAGCCTACGATTCAACAACGTCAAGACATCTGCGACGAGTTAGCCAAGACACCGTTTTGGAGTCTACTAAAAGTGTACATGGATGACATATTGATCGCCACGGATAGAAAGAAGAAGTCCGGTATGGACTTAATAAAGATCATGCAATGCTACAACTCAAAGGAGCAAAAATTCAAGTTTGGAGATAATGAAGCAAAGGGAATAACAAACGATGATGTTGTTCAGATATTTGGTCTGAACAACAGAGGAGTGGAGCTTCCAAACACAGACAAGTCCACGAAACATGGAAAAGAGGACCCCTTCGTTGACAAATATTTTAAGCATGCAAAAGTTGTCAAAAGGAAGCAACTAATAGAAGCCTATGAAAAATCACTCCTGGACCAATCTCCTCAAGGTGCAAAAGATACTACTTCCATAATCTGTGCTCATCTCATCCAATCACTTCTTTTTGCTAATTCTGGCACGTATATTACCTGGAGTTTCTTAAAGATATGTGGGGAGTTGGATGAGATCAGTAAATATAACTGGGCGAAAGGAGTGAAAGATTACCTCCTCAAGATGATAAACAAAGCACAGAAGAAGAAAAAAGATAGAGGAGAAGAACCAACAATTAGCGGCTGCACCGTCTTTGTTTTGTATTGGATATGTCTTCATACAAATGTGGTTAAGACAGTCAAAGGACGAGAAAACATGATTCCGGCAATTGCAAGATGGGATACAAGTATGATACACAAAGCAATCAAAGATGTCCCAGTTGAACAAATCAAGATTGATGGTGTCATTCCTTGTGCTCAACCACAAGAAGAATCTTCGAAGAAAAAACAAGAAAAAACAAGAACAAAGAAAAGAAAGCAAAGTGATGTCATTCCTTATGCTCAACCTGAAGATGATAAATTGGATGATCATTCCCACCAAAAAGAGGAAGCAAACAAAGATGAGATGGCAGAGGTTGATAAAAATTTGAAGAAGAAAAAGAACAAACAGAAAAGGAAGCAAAATGATGAAGAACATGAAAGCATCAATATAAATGAAGCTCAAGCACAGTTCGATCAACTGTGCAAAGAGGAAATGAGCAAACTCGATGCCATTCATCAGAGATACATCGTTGGCATGGATAGAACTTTAAGTGTAGATCTTTTCAATGAGTTCAAGAGTGCTAGGAGCATTGATCAGAAAAGGTACCTAACCTTCAAGACGATGATGGCATACGCACTTGACTCTTCCAACAAAGAGTGCGTAGCACTCAGGGAATCAAATAAAATGCTAAACCATGAAATGAAAAAGATTCTACGACAAGAGGAGCTTGACAAGAAGAAAGTGAATGCACTAAATGCTGATTTATTATCATTGCAGACAAAGAACAGTGCAGCTTCTCTAAAAAATCAATGA

Protein Analysis

553

Amino Acids

64.04

Weight (kDa)

9.39

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 157 - 333 1.7e-06 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1449
AccB1I GGYRCC 1 cut(s) 1449
AccB7I CCANNNNNTGG 1 cut(s) 78
AccI GTMKAC 1 cut(s) 334
AciI CCGC 2 cut(s) 112, 885
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 3 cut(s) 90, 440, 1228
AcuI CTGAAG 1 cut(s) 1173
AfaI GTAC 3 cut(s) 269, 347, 1451
AfiI CCNNNNNNNGG 2 cut(s) 78, 1189
AflII CTTAAG 1 cut(s) 10
AgsI TTSAA 8 cut(s) 123, 284, 445, 1028, 1234, 1411, 1420, 1462
AjnI CCWGG 2 cut(s) 645, 743
AluBI AGCT 5 cut(s) 9, 526, 1304, 1573, 1640
AluI AGCT 5 cut(s) 9, 526, 1304, 1573, 1640
Alw21I GWGCWC 2 cut(s) 697, 1063
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 888, 1637
ApoI RAATTY 3 cut(s) 90, 440, 1228
Asp718I GGTACC 1 cut(s) 1449
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 565, 649
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 16
AsuII TTCGAA 1 cut(s) 1082
AvaII GGWCC 2 cut(s) 565, 649
BanI GGYRCC 1 cut(s) 1449
BarI GAAGNNNNNNTAC 2 cut(s) 1442, 1474
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 2 cut(s) 697, 1063
BbvI GCAGC 2 cut(s) 875, 1649
BccI CCATC 5 cut(s) 235, 975, 1037, 1204, 1465
BciT130I CCWGG 2 cut(s) 647, 745
BciVI GTATCC 1 cut(s) 979
BclI TGATCA 2 cut(s) 1171, 1438
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 1 cut(s) 1428
BfrI CTTAAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 979
BglII AGATCT 1 cut(s) 1402
BisI GCNGC 3 cut(s) 886, 889, 1638
BlsI GCNGC 3 cut(s) 887, 890, 1639
Bme1390I CCNGG 3 cut(s) 43, 647, 745
Bme18I GGWCC 2 cut(s) 565, 649
BmgT120I GGNCC 2 cut(s) 565, 649
BmiI GGNNCC 2 cut(s) 567, 1451
BmrFI CCNGG 3 cut(s) 43, 647, 745
BmrI ACTGGG 3 cut(s) 66, 805, 1016
BmsI GCATC 4 cut(s) 221, 244, 1296, 1342
BmuI ACTGGG 3 cut(s) 66, 805, 1016
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 766
Bpu14I TTCGAA 1 cut(s) 1082
BpuEI CTTGAG 3 cut(s) 645, 809, 1290
BpuMI CCSGG 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 42, 167, 369
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 1189
Bse1I ACTGG 3 cut(s) 72, 800, 1022
Bse3DI GCAATG 2 cut(s) 422, 1616
BseBI CCWGG 2 cut(s) 647, 745
BseDI CCNNGG 3 cut(s) 42, 167, 369
BseGI GGATG 5 cut(s) 153, 358, 702, 781, 1174
BseLI CCNNNNNNNGG 2 cut(s) 78, 1189
BseMI GCAATG 2 cut(s) 422, 1616
BseMII CTCAG 1 cut(s) 1528
BseNI ACTGG 3 cut(s) 72, 800, 1022
BseRI GAGGAG 5 cut(s) 531, 648, 812, 879, 1583
BseXI GCAGC 2 cut(s) 875, 1649
BsgI GTGCAG 2 cut(s) 874, 1656
BshNI GGYRCC 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 697, 1063
BsiSI CCGG 3 cut(s) 42, 390, 968
BslFI GGGAC 2 cut(s) 95, 1004
BslI CCNNNNNNNGG 2 cut(s) 78, 1189
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 2 cut(s) 95, 1004
BsmI GAATGC 1 cut(s) 1597
Bsp119I TTCGAA 1 cut(s) 1082
Bsp1286I GDGCHC 2 cut(s) 697, 1063
Bsp1407I TGTACA 2 cut(s) 267, 345
Bsp143I GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
BspACI CCGC 2 cut(s) 112, 885
BspCNI CTCAG 1 cut(s) 1527
BspLI GGNNCC 2 cut(s) 567, 1451
BspPI GGATC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 1449
BspTI CTTAAG 1 cut(s) 10
BsrDI GCAATG 2 cut(s) 422, 1616
BsrGI TGTACA 2 cut(s) 267, 345
BsrI ACTGG 3 cut(s) 72, 800, 1022
BssECI CCNNGG 3 cut(s) 42, 167, 369
BssMI GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
Bst2UI CCWGG 2 cut(s) 647, 745
Bst4CI ACNGT 7 cut(s) 36, 324, 895, 943, 1314, 1326, 1634
Bst6I CTCTTC 1 cut(s) 1495
BstAFI CTTAAG 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 2 cut(s) 267, 345
BstBAI YACGTR 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 2 cut(s) 116, 594
BstDEI CTNAG 2 cut(s) 186, 1514
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 5 cut(s) 153, 358, 702, 781, 1174
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 8 cut(s) 106, 366, 411, 783, 1174, 1321, 1405, 1441
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
BstMWI GCNNNNNNNGC 1 cut(s) 1601
BstNI CCWGG 2 cut(s) 647, 745
BstNSI RCATGY 2 cut(s) 118, 596
BstSCI CCNGG 3 cut(s) 41, 645, 743
BstV1I GCAGC 2 cut(s) 875, 1649
BstV2I GAAGAC 1 cut(s) 911
BstX2I RGATCY 2 cut(s) 103, 1402
BstYI RGATCY 2 cut(s) 103, 1402
BsuI GTATCC 1 cut(s) 979
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 5 cut(s) 153, 358, 702, 781, 1174
BtsIMutI CAGTG 1 cut(s) 1639
Cac8I GCNNGC 2 cut(s) 116, 594
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 2 cut(s) 565, 649
Csp6I GTAC 3 cut(s) 268, 346, 1450
CviQI GTAC 3 cut(s) 268, 346, 1450
DdeI CTNAG 2 cut(s) 186, 1514
DpnI GATC 8 cut(s) 105, 365, 410, 782, 1173, 1320, 1404, 1440
DpnII GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
Eam1104I CTCTTC 1 cut(s) 1495
EarI CTCTTC 1 cut(s) 1495
Eco47I GGWCC 2 cut(s) 565, 649
Eco57I CTGAAG 1 cut(s) 1173
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 2 cut(s) 645, 743
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 1557, 1589
FaqI GGGAC 2 cut(s) 95, 1004
FbaI TGATCA 2 cut(s) 1171, 1438
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 886, 889, 1638
FokI GGATG 5 cut(s) 160, 365, 689, 788, 1181
Fsp4HI GCNGC 3 cut(s) 886, 889, 1638
FspBI CTAG 1 cut(s) 1428
GlaI GCGC 1 cut(s) 20
GluI GCNGC 3 cut(s) 886, 889, 1638
GsuI CTGGAG 1 cut(s) 766
HapII CCGG 3 cut(s) 42, 390, 968
HhaI GCGC 1 cut(s) 21
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HinfI GANTC 7 cut(s) 280, 331, 964, 1076, 1487, 1520, 1555
HpaII CCGG 3 cut(s) 42, 390, 968
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 4 cut(s) 335, 346, 546, 577
Hpy188I TCNGA 4 cut(s) 495, 507, 1365, 1443
Hpy188III TCNNGA 6 cut(s) 101, 293, 826, 1036, 1420, 1462
Hpy8I GTNNAC 4 cut(s) 335, 346, 546, 577
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 4 cut(s) 129, 205, 580, 1468
HpyCH4III ACNGT 7 cut(s) 36, 324, 895, 943, 1314, 1326, 1634
HpyCH4IV ACGT 2 cut(s) 289, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 1601
HpyF3I CTNAG 2 cut(s) 186, 1514
HpySE526I ACGT 2 cut(s) 289, 734
HspAI GCGC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 1453
Ksp22I TGATCA 2 cut(s) 1171, 1438
Kzo9I GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
LmnI GCTCC 4 cut(s) 433, 523, 1431, 1570
Lsp1109I GCAGC 2 cut(s) 875, 1649
LweI GCATC 4 cut(s) 221, 244, 1296, 1342
MaeI CTAG 1 cut(s) 1428
MaeII ACGT 2 cut(s) 289, 734
MalI GATC 8 cut(s) 105, 365, 410, 782, 1173, 1320, 1404, 1440
MboI GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
MfeI CAATTG 2 cut(s) 263, 972
MflI RGATCY 2 cut(s) 103, 1402
MhlI GDGCHC 2 cut(s) 697, 1063
MluCI AATT 8 cut(s) 90, 263, 440, 724, 879, 972, 1163, 1228
MlyI GAGTC 2 cut(s) 340, 1481
MmeI TCCRAC 2 cut(s) 753, 1518
MseI TTAA 6 cut(s) 11, 401, 588, 755, 936, 1394
MslI CAYNNNNRTG 1 cut(s) 927
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 3 cut(s) 42, 390, 968
MspR9I CCNGG 3 cut(s) 43, 647, 745
MunI CAATTG 2 cut(s) 263, 972
Mva1269I GAATGC 1 cut(s) 1597
MvaI CCWGG 2 cut(s) 647, 745
MwoI GCNNNNNNNGC 1 cut(s) 1601
NciI CCSGG 1 cut(s) 43
NdeII GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
NlaIV GGNNCC 2 cut(s) 567, 1451
NspI RCATGY 2 cut(s) 118, 596
NspV TTCGAA 1 cut(s) 1082
PaeI GCATGC 2 cut(s) 118, 596
PctI GAATGC 1 cut(s) 1597
PfeI GAWTC 5 cut(s) 280, 964, 1076, 1520, 1555
PflMI CCANNNNNTGG 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 3 cut(s) 887, 890, 1639
PleI GAGTC 2 cut(s) 339, 1481
PpsI GAGTC 2 cut(s) 339, 1481
Ppu21I YACGTR 1 cut(s) 735
PpuMI RGGWCCY 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 645, 743
PspGI CCWGG 2 cut(s) 645, 743
PspN4I GGNNCC 2 cut(s) 567, 1451
PspPI GGNCC 2 cut(s) 565, 649
PspPPI RGGWCCY 1 cut(s) 565
PsuI RGATCY 2 cut(s) 103, 1402
RsaI GTAC 3 cut(s) 269, 347, 1451
RsaNI GTAC 3 cut(s) 268, 346, 1450
RseI CAYNNNNRTG 1 cut(s) 927
SaqAI TTAA 6 cut(s) 11, 401, 588, 755, 936, 1394
SatI GCNGC 3 cut(s) 886, 889, 1638
Sau3AI GATC 8 cut(s) 103, 363, 408, 780, 1171, 1318, 1402, 1438
Sau96I GGNCC 2 cut(s) 565, 649
SchI GAGTC 2 cut(s) 340, 1481
ScrFI CCNGG 3 cut(s) 43, 647, 745
SduI GDGCHC 2 cut(s) 697, 1063
SfaNI GCATC 4 cut(s) 221, 244, 1296, 1342
SfuI TTCGAA 1 cut(s) 1082
SinI GGWCC 2 cut(s) 565, 649
SmiMI CAYNNNNRTG 1 cut(s) 927
SmlI CTYRAG 4 cut(s) 10, 660, 824, 1305
SmoI CTYRAG 4 cut(s) 10, 660, 824, 1305
SphI GCATGC 2 cut(s) 118, 596
Sse9I AATT 8 cut(s) 90, 263, 440, 724, 879, 972, 1163, 1228
SsiI CCGC 2 cut(s) 112, 885
SspI AATATT 1 cut(s) 584
SspMI CTAG 1 cut(s) 1428
StyD4I CCNGG 3 cut(s) 41, 645, 743
TaaI ACNGT 7 cut(s) 36, 324, 895, 943, 1314, 1326, 1634
TaiI ACGT 2 cut(s) 292, 737
TaqI TCGA 3 cut(s) 1082, 1317, 1350
TasI AATT 8 cut(s) 90, 263, 440, 724, 879, 972, 1163, 1228
TatI WGTACW 2 cut(s) 267, 345
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 5 cut(s) 280, 964, 1076, 1520, 1555
Tru1I TTAA 6 cut(s) 11, 401, 588, 755, 936, 1394
Tru9I TTAA 6 cut(s) 11, 401, 588, 755, 936, 1394
TscAI CASTG 1 cut(s) 1639
TseI GCWGC 2 cut(s) 888, 1637
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 1 cut(s) 1639
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 565, 649
XapI RAATTY 3 cut(s) 90, 440, 1228
XceI RCATGY 2 cut(s) 118, 596
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 1 cut(s) 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.