Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006052

Physical Location & Seq

Genomic Coordinates Forward (+)

31476344 .. 31484379

Gene Structure 8036 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1186700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGAGGGTAAAGAAGATGCCGAAGCATACGAAGAGCAGCCTCCGTCGCCTATTCAGGTTTTGCAGGAAATATCTGAGGAGGCACTTAAAGTGGCAGGGGAGACACTGCAAAATGTGGTCCCGGGTAATTCGAGCATGCCACCATTGGCACCGGGGCATAGGCGCTCCCGAAGTGAAATTGTAACTACGGGACACAGACGGAGCAATAGCTTCCAGAAATTGAAAAATCAGATGCAGAGGGCGTGGAGATGGGGTGGAAATTCGCGGGATGATTTGCGGTTGGCTTTCAATCCTGAGGTTTTGGCAAACCAAAAACGTCAGTGGTATCAGCTTCACTCCAAATCAAAGGGCCATATAAGATATACCGAGCCAACTTCACTCTTTGAGCATTTTATTATTGCGGGGCTTCATCCGGATGCTAACCTTGAAACTGTGGAGGATGCATTTGTTAAAAGAAAGAAATGGGAGATGGAGATGATAAACTCTGGAATTGTTGACCTTAAATTGCTACAGCAGCGGGGACCTCCAATTCCTACATTAGAACCTCAGATACTTTTCAAATATCCTCCTGGGAAGCGTCTAGATATGCGTTTGAAAGATTTAGCTTCCTTTTGTTTTCCTGGAGGGGTTAAGGCACGGTTAATGGAGAAGACTCCATCACTAAGTGATTTAAATCAAGTTGTTTACGGACAGGAGCATTTAGGAAAAGATGATTTATCATTTATTTTCTCACTCAAGGTGGCAGACAATGCAACACTTTATGGTGTTTGCCTACACGTGTCAGAAATTGTTCAGAGGCCTCCTGCTATCTTAGGCATCTCATCACCTAATTCTCATTCGATGGGAGGACTCTTCCGATTTTTGGTTTCTGCGCCTCGATGCTACTGTGTGCTATCCAGAGTTCCTTTTTTTGAGTTACACTACGAGATGCTGAATAGTATCGTTCAACAGGAGCGTCTGAAGCGAATCACACAATTTGCTAGTGAAATGGCACTTACTGATTTTGTCCCTACATTGCCCAATGTACAAGATAATGATGATGAGTCCCCCGAGAGGGAGTCTTTTGATAATTGGATGGACTCTGCAATACCAGTTGACAGTCCAATTGCCCTTACAGCTGCCAGTGCAGGAATTAGACTGGACGATGAGACTCCACCTTCTTCACTCAAGATATGGGAACTGCAGTCCCCTGAAAGTGTCTCAGCTAGTGAGAGTTCAGATTTTAGTCAAGTACGGTATTTAGACAAGGATGGTAGAAAGGATTCACAATGTTCTGATGACTATGGTTTTGAGGTCTCAGAAACTCGTTCGGAAGCTTCAGAAAGAATATGCGGAAACTATGGAAATGACCATACTTTTTCAGAGGTTGGGACATCTTTCTCCTCCAGAAATCGCACGTTTGAGAGTCTTGGGAGTGCTGAATCTGTATTTAGAAATGGCCATACTTCTCCAGAGGTTGGGCCATCTTTCTCTTCCAGGAACCGCACATTGGAGCGTCTTGGCAGTTCCGAATCTTTGTTCAGTCCAGCTAGAAGCATGGTATCGGAGGATGAAGACGATGACCTTTTCTCGACTTGTGAGAAAGAGTTTGGTGATGAACTGATAATGGAATGGGCTAGGGAGAACAAGAACGATTTGTTACAGATTGTTTGTGGTTTTCATGCTCTGCCTCTCCCACAACGAGGAAGTGAATTATCGTTTCTACCTCTTGAACATCTTCAAGCTACTGAGTATAGGCGACCTCCAGTAGCTGCTCTTGGTTTTGATGAGAATAGTTTAGATTCATTTCAAGATCCTGGGGTCGATGTTATGCTAGCTGCTGCTGAAGAAGCGCTTGCACTATCAATATGGACAACTGCAACAATCTGCCGAGTTCTCTCCCTTGAAAGTATTTTATCATTGCTTACGGGAGTATTACTAGAAAAACAAGTGGTAATAGTGTGTCCGAATCTGGGTGTGCTATCAGCTACAGTATTATCTCTCATTCCCATGATTCGTCCATTTCAATGGCAGAGTTTAATGCTTCCTGTTCTACCAGGGAAAATGCTTGACTTTCTTGACGCCCCAGTTCCATTTATTACTGGGATACAACAGTTTCCTGCAGACCTGAAGAGGAAAACATCGGATCCTGTTCAAGTTAATGTGCAAAAGGATCAGGTGAAAATGTGTCATTTGCCGTCACTTCCAAGACATAAAGATCTAGCCACTGAATTAGGGCCCATCCATGCTAGACTGTCATGTGAAGGTTCATTTGCTAAAAAGCATCCTGTATATAGGTGCAACGAAGTGCAGGTCGAAGCTGCAGGTCAGTTTTTGGAAGTCATGAAGAACTACATGGAGTCACTTTGTTCAGATCTAAGGTCTTACACAATAACGAGTGTACAATCAAACAGTGACAGGGTTTCTTTACTTCTTAAAGAGAGCTTTATCGATTCCTTTCCTAGCAAGGACAGGCAATTTATCAAGTTATTTGTGGACACGCAGATGTTCACTGTTCTATCCGACTCTCGTTTGTCAAGCTTTGAGAACGGTGGCTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN uDENN

Protein Analysis

847

Amino Acids

94.77

Weight (kDa)

5.19

Isoelectric Point (pI)

50.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	182 - 259	5.9e-07	uDENN domain
DENN	PF02141	621 - 715	1.8e-20	DENN (AEX-3) domain
dDENN	PF03455	806 - 839	5.5e-06	dDENN domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006052)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20320 AT2G20320
fragaria_vesca	FvH4_2g20280
malus_domestica	MD05G1186700.v1.1 MD10G1174800.v1.1
prunus_persica	Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1 Prupe.8G213500_v2.0.a1
pyrus_communis	pycom05g17090 pycom10g15050
rosa_chinensis	RchiOBHm_Chr6g0286081
rosa_laevigata	RLG00000012584
rosa_multiflora	Rmu_sc0023663.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182470
rosa_rugosa	Rorug06G0178800
rosa_samantha	Rh3BG272300 Rh6AG291200 Rh6BG294500 Rh6CG294100 Rh6DG286100
rosa_wichuraiana	Rw6G025090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	2281, 2294
AccB1I	GGYRCC	1 cut(s)	148
AccB7I	CCANNNNNTGG	1 cut(s)	1457
AccII	CGCG	1 cut(s)	265
AccIII	TCCGGA	1 cut(s)	412
AciI	CCGC	6 cut(s)	265, 277, 401, 517, 1332, 1483
AclWI	GGATC	4 cut(s)	1787, 2120, 2133, 2160
AcoI	YGGCCR	1 cut(s)	1438
AcsI	RAATTY	1 cut(s)	259
AcuI	CTGAAG	4 cut(s)	980, 1302, 1845, 2129
AcvI	CACGTG	1 cut(s)	778
AcyI	GRCGYC	1 cut(s)	2061
AdeI	CACNNNGTG	1 cut(s)	665
AfaI	GTAC	3 cut(s)	1026, 1233, 2382
AfeI	AGCGCT	1 cut(s)	1833
AfiI	CCNNNNNNNGG	7 cut(s)	862, 1053, 1054, 1457, 1489, 1682, 1952
AflIII	ACRYGT	2 cut(s)	775, 777
AjnI	CCWGG	5 cut(s)	568, 619, 1475, 1795, 2035
AjuI	GAANNNNNNNTTGG	2 cut(s)	1472, 1504
AloI	GAACNNNNNNTCC	1 cut(s)	2519
Alw26I	GTCTC	4 cut(s)	95, 1142, 1204, 1300
AlwI	GGATC	4 cut(s)	1787, 2120, 2133, 2160
AlwNI	CAGNNNCTG	1 cut(s)	1751
Ama87I	CYCGRG	2 cut(s)	121, 1049
Aor13HI	TCCGGA	1 cut(s)	412
Aor51HI	AGCGCT	1 cut(s)	1833
AoxI	GGCC	5 cut(s)	349, 797, 1438, 1460, 2216
ApaI	GGGCCC	1 cut(s)	2220
ApeKI	GCWGC	7 cut(s)	37, 514, 1118, 1751, 1817, 1820, 2300
ApoI	RAATTY	1 cut(s)	259
ArsI	GACNNNNNNTTYG	4 cut(s)	1271, 1303, 2277, 2309
Asp700I	GAANNNNTTC	3 cut(s)	789, 1261, 1716
AspLEI	GCGC	3 cut(s)	165, 874, 1834
AspS9I	GGNCC	6 cut(s)	118, 349, 521, 1460, 2216, 2217
AsuC2I	CCSGG	3 cut(s)	122, 123, 153
AsuHPI	GGTGA	3 cut(s)	816, 1604, 2170
AsuNHI	GCTAGC	1 cut(s)	1813
AvaI	CYCGRG	2 cut(s)	121, 1049
AvaII	GGWCC	2 cut(s)	118, 521
AxyI	CCTNAGG	1 cut(s)	294
BaeGI	GKGCMC	1 cut(s)	2220
BaeI	ACNNNNGTAYC	2 cut(s)	2078, 2111
BalI	TGGCCA	1 cut(s)	1440
BamHI	GGATCC	1 cut(s)	2125
BanI	GGYRCC	1 cut(s)	148
BanII	GRGCYC	1 cut(s)	2220
BbrPI	CACGTG	1 cut(s)	778
BbsI	GAAGAC	2 cut(s)	656, 1560
BbvI	GCAGC	7 cut(s)	49, 526, 1105, 1738, 1804, 1807, 2287
BccI	CCATC	8 cut(s)	243, 463, 664, 835, 1069, 1244, 1471, 2228
BceAI	ACGGC	1 cut(s)	2161
BciT130I	CCWGG	5 cut(s)	570, 621, 1477, 1797, 2037
BciVI	GTATCC	1 cut(s)	2079
BcnI	CCSGG	3 cut(s)	122, 123, 153
BcoDI	GTCTC	4 cut(s)	95, 1142, 1204, 1300
BfmI	CTRYAG	5 cut(s)	509, 1181, 1968, 2100, 2301
BfoI	RGCGCY	2 cut(s)	166, 1835
BfuAI	ACCTGC	2 cut(s)	2281, 2294
BfuI	GTATCC	1 cut(s)	2079
BglII	AGATCT	2 cut(s)	2197, 2353
BisI	GCNGC	7 cut(s)	38, 515, 1119, 1752, 1818, 1821, 2301
BlsI	GCNGC	7 cut(s)	39, 516, 1120, 1753, 1819, 1822, 2302
Bme1390I	CCNGG	8 cut(s)	122, 123, 153, 570, 621, 1477, 1797, 2037
Bme18I	GGWCC	2 cut(s)	118, 521
BmeT110I	CYCGRG	2 cut(s)	121, 1049
BmgT120I	GGNCC	6 cut(s)	118, 349, 521, 1460, 2216, 2217
BmiI	GGNNCC	7 cut(s)	120, 150, 522, 1481, 2127, 2218, 2536
BmrFI	CCNGG	8 cut(s)	122, 123, 153, 570, 621, 1477, 1797, 2037
BmrI	ACTGGG	2 cut(s)	2060, 2091
BmsI	GCATC	8 cut(s)	7, 222, 406, 430, 825, 869, 918, 2272
BmtI	GCTAGC	1 cut(s)	1817
BmuI	ACTGGG	2 cut(s)	2060, 2091
BpiI	GAAGAC	2 cut(s)	656, 1560
BpmI	CTGGAG	4 cut(s)	642, 1369, 1434, 1728
BpuEI	CTTGAG	2 cut(s)	719, 1151
BpuMI	CCSGG	3 cut(s)	122, 123, 153
Bsa29I	ATCGAT	1 cut(s)	2430
BsaAI	YACGTR	1 cut(s)	778
BsaBI	GATNNNNATC	1 cut(s)	672
BsaHI	GRCGYC	1 cut(s)	2061
BsaI	GGTCTC	1 cut(s)	1300
BsaJI	CCNNGG	5 cut(s)	121, 152, 569, 1796, 2036
BsaWI	WCCGGW	1 cut(s)	412
BsaXI	ACNNNNNCTCC	3 cut(s)	238, 268, 2519
Bsc4I	CCNNNNNNNGG	7 cut(s)	862, 1053, 1054, 1457, 1489, 1682, 1952
Bse1I	ACTGG	6 cut(s)	1091, 1122, 1143, 1745, 2066, 2086
Bse21I	CCTNAGG	1 cut(s)	294
Bse3DI	GCAATG	2 cut(s)	1013, 1898
Bse8I	GATNNNNATC	1 cut(s)	672
BseAI	TCCGGA	1 cut(s)	412
BseBI	CCWGG	5 cut(s)	570, 621, 1477, 1797, 2037
BseCI	ATCGAT	1 cut(s)	2430
BseDI	CCNNGG	5 cut(s)	121, 152, 569, 1796, 2036
BseGI	GGATG	9 cut(s)	274, 409, 421, 445, 1080, 1255, 1555, 2220, 2263
BseJI	GATNNNNATC	1 cut(s)	672
BseLI	CCNNNNNNNGG	7 cut(s)	862, 1053, 1054, 1457, 1489, 1682, 1952
BseMI	GCAATG	2 cut(s)	1013, 1898
BseMII	CTCAG	6 cut(s)	66, 285, 560, 1215, 1311, 1719
BseNI	ACTGG	6 cut(s)	1091, 1122, 1143, 1745, 2066, 2086
BseRI	GAGGAG	2 cut(s)	92, 1372
BseSI	GKGCMC	1 cut(s)	2220
BseXI	GCAGC	7 cut(s)	49, 526, 1105, 1738, 1804, 1807, 2287
BsgI	GTGCAG	2 cut(s)	1146, 2309
Bsh1236I	CGCG	1 cut(s)	265
BshFI	GGCC	5 cut(s)	351, 799, 1440, 1462, 2218
BshNI	GGYRCC	1 cut(s)	148
BshVI	ATCGAT	1 cut(s)	2430
BsiHKCI	CYCGRG	2 cut(s)	121, 1049
BsiSI	CCGG	3 cut(s)	122, 152, 413
BslFI	GGGAC	7 cut(s)	104, 204, 534, 992, 1030, 1171, 1384
BslI	CCNNNNNNNGG	7 cut(s)	862, 1053, 1054, 1457, 1489, 1682, 1952
BsmAI	GTCTC	4 cut(s)	95, 1142, 1204, 1300
BsmFI	GGGAC	7 cut(s)	104, 204, 534, 992, 1030, 1171, 1384
BsnI	GGCC	5 cut(s)	351, 799, 1440, 1462, 2218
Bso31I	GGTCTC	1 cut(s)	1300
BsoBI	CYCGRG	2 cut(s)	121, 1049
Bsp120I	GGGCCC	1 cut(s)	2216
Bsp1286I	GDGCHC	1 cut(s)	2220
Bsp13I	TCCGGA	1 cut(s)	412
Bsp1407I	TGTACA	2 cut(s)	1024, 2380
Bsp143I	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
BspACI	CCGC	6 cut(s)	265, 277, 401, 517, 1332, 1483
BspANI	GGCC	5 cut(s)	351, 799, 1440, 1462, 2218
BspCNI	CTCAG	6 cut(s)	67, 286, 559, 1214, 1310, 1720
BspDI	ATCGAT	1 cut(s)	2430
BspEI	TCCGGA	1 cut(s)	412
BspFNI	CGCG	1 cut(s)	265
BspHI	TCATGA	1 cut(s)	2322
BspLI	GGNNCC	7 cut(s)	120, 150, 522, 1481, 2127, 2218, 2536
BspMAI	CTGCAG	3 cut(s)	1185, 2104, 2305
BspMI	ACCTGC	2 cut(s)	2281, 2294
BspOI	GCTAGC	1 cut(s)	1817
BspPI	GGATC	4 cut(s)	1787, 2120, 2133, 2160
BspQI	GCTCTTC	1 cut(s)	27
BspT107I	GGYRCC	1 cut(s)	148
BspTNI	GGTCTC	1 cut(s)	1300
BsrDI	GCAATG	2 cut(s)	1013, 1898
BsrGI	TGTACA	2 cut(s)	1024, 2380
BsrI	ACTGG	6 cut(s)	1091, 1122, 1143, 1745, 2066, 2086
BssECI	CCNNGG	5 cut(s)	121, 152, 569, 1796, 2036
BssMI	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
BssNI	GRCGYC	1 cut(s)	2061
Bst2UI	CCWGG	5 cut(s)	570, 621, 1477, 1797, 2037
Bst6I	CTCTTC	4 cut(s)	27, 857, 1477, 2105
BstACI	GRCGYC	1 cut(s)	2061
BstAPI	GCANNNNNTGC	1 cut(s)	749
BstAUI	TGTACA	2 cut(s)	1024, 2380
BstBAI	YACGTR	1 cut(s)	778
BstC8I	GCNNGC	3 cut(s)	137, 1815, 1836
BstDEI	CTNAG	9 cut(s)	75, 294, 546, 662, 811, 1201, 1297, 1728, 2357
BstF5I	GGATG	9 cut(s)	274, 409, 421, 445, 1080, 1255, 1555, 2220, 2263
BstFNI	CGCG	1 cut(s)	265
BstH2I	RGCGCY	2 cut(s)	166, 1835
BstHHI	GCGC	3 cut(s)	165, 874, 1834
BstKTI	GATC	5 cut(s)	1795, 2128, 2155, 2200, 2356
BstMAI	GTCTC	4 cut(s)	95, 1142, 1204, 1300
BstMBI	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
BstMWI	GCNNNNNNNGC	7 cut(s)	46, 514, 749, 961, 1115, 1124, 1829
BstNI	CCWGG	5 cut(s)	570, 621, 1477, 1797, 2037
BstNSI	RCATGY	1 cut(s)	139
BstSCI	CCNGG	8 cut(s)	120, 121, 151, 568, 619, 1475, 1795, 2035
BstSFI	CTRYAG	5 cut(s)	509, 1181, 1968, 2100, 2301
BstSLI	GKGCMC	1 cut(s)	2220
BstUI	CGCG	1 cut(s)	265
BstV1I	GCAGC	7 cut(s)	49, 526, 1105, 1738, 1804, 1807, 2287
BstV2I	GAAGAC	2 cut(s)	656, 1560
BstX2I	RGATCY	4 cut(s)	1792, 2125, 2197, 2353
BstXI	CCANNNNNNTGG	1 cut(s)	2007
BstYI	RGATCY	4 cut(s)	1792, 2125, 2197, 2353
Bsu15I	ATCGAT	1 cut(s)	2430
Bsu36I	CCTNAGG	1 cut(s)	294
BsuI	GTATCC	1 cut(s)	2079
BsuRI	GGCC	5 cut(s)	351, 799, 1440, 1462, 2218
BsuTUI	ATCGAT	1 cut(s)	2430
BtsCI	GGATG	9 cut(s)	274, 409, 421, 445, 1080, 1255, 1555, 2220, 2263
BtsI	GCAGTG	1 cut(s)	104
BtsIMutI	CAGTG	6 cut(s)	104, 326, 1129, 2205, 2398, 2490
BveI	ACCTGC	2 cut(s)	2281, 2294
Cac8I	GCNNGC	3 cut(s)	137, 1815, 1836
CaiI	CAGNNNCTG	1 cut(s)	1751
CciI	TCATGA	1 cut(s)	2322
CfoI	GCGC	3 cut(s)	165, 874, 1834
Cfr13I	GGNCC	6 cut(s)	118, 349, 521, 1460, 2216, 2217
Cfr9I	CCCGGG	1 cut(s)	121
ClaI	ATCGAT	1 cut(s)	2430
CseI	GACGC	4 cut(s)	566, 944, 1484, 2069
Csp6I	GTAC	3 cut(s)	1025, 1232, 2381
CviAII	CATG	8 cut(s)	136, 1537, 1661, 1990, 2225, 2238, 2323, 2335
CviQI	GTAC	3 cut(s)	1025, 1232, 2381
DdeI	CTNAG	9 cut(s)	75, 294, 546, 662, 811, 1201, 1297, 1728, 2357
DpnI	GATC	5 cut(s)	1794, 2127, 2154, 2199, 2355
DpnII	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
DraI	TTTAAA	1 cut(s)	672
DraIII	CACNNNGTG	1 cut(s)	665
EaeI	YGGCCR	1 cut(s)	1438
Eam1104I	CTCTTC	4 cut(s)	27, 857, 1477, 2105
EarI	CTCTTC	4 cut(s)	27, 857, 1477, 2105
Eco147I	AGGCCT	1 cut(s)	799
Eco24I	GRGCYC	1 cut(s)	2220
Eco31I	GGTCTC	1 cut(s)	1300
Eco47I	GGWCC	2 cut(s)	118, 521
Eco47III	AGCGCT	1 cut(s)	1833
Eco57I	CTGAAG	4 cut(s)	980, 1302, 1845, 2129
Eco72I	CACGTG	1 cut(s)	778
Eco81I	CCTNAGG	1 cut(s)	294
Eco88I	CYCGRG	2 cut(s)	121, 1049
EcoO109I	RGGNCCY	2 cut(s)	521, 2216
EcoRII	CCWGG	5 cut(s)	568, 619, 1475, 1795, 2035
EcoT22I	ATGCAT	1 cut(s)	445
EcoT38I	GRGCYC	1 cut(s)	2220
FaeI	CATG	8 cut(s)	139, 1540, 1664, 1993, 2228, 2241, 2326, 2338
FalI	AAGNNNNNCTT	2 cut(s)	1818, 1850
FaqI	GGGAC	7 cut(s)	104, 204, 534, 992, 1030, 1171, 1384
FatI	CATG	8 cut(s)	135, 1536, 1660, 1989, 2224, 2237, 2322, 2334
FauI	CCCGC	3 cut(s)	258, 394, 510
Fnu4HI	GCNGC	7 cut(s)	38, 515, 1119, 1752, 1818, 1821, 2301
FokI	GGATG	9 cut(s)	281, 396, 428, 452, 1087, 1262, 1562, 2207, 2250
FriOI	GRGCYC	1 cut(s)	2220
Fsp4HI	GCNGC	7 cut(s)	38, 515, 1119, 1752, 1818, 1821, 2301
GlaI	GCGC	3 cut(s)	164, 873, 1833
GluI	GCNGC	7 cut(s)	38, 515, 1119, 1752, 1818, 1821, 2301
GsuI	CTGGAG	4 cut(s)	642, 1369, 1434, 1728
HaeII	RGCGCY	2 cut(s)	166, 1835
HaeIII	GGCC	5 cut(s)	351, 799, 1440, 1462, 2218
HapII	CCGG	3 cut(s)	122, 152, 413
HgaI	GACGC	4 cut(s)	566, 944, 1484, 2069
HhaI	GCGC	3 cut(s)	165, 874, 1834
Hin1I	GRCGYC	1 cut(s)	2061
Hin1II	CATG	8 cut(s)	139, 1540, 1664, 1993, 2228, 2241, 2326, 2338
Hin6I	GCGC	3 cut(s)	163, 872, 1832
HinP1I	GCGC	3 cut(s)	163, 872, 1832
HincII	GTYRAC	2 cut(s)	496, 1096
HindII	GTYRAC	2 cut(s)	496, 1096
HindIII	AAGCTT	2 cut(s)	1314, 2518
HpaII	CCGG	3 cut(s)	122, 152, 413
HphI	GGTGA	3 cut(s)	816, 1604, 2170
Hpy166II	GTNNAC	6 cut(s)	496, 685, 1096, 2381, 2476, 2490
Hpy8I	GTNNAC	6 cut(s)	496, 685, 1096, 2381, 2476, 2490
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	2 cut(s)	1167, 2237
HpyCH4IV	ACGT	3 cut(s)	316, 777, 1397
HpyF10VI	GCNNNNNNNGC	7 cut(s)	46, 514, 749, 961, 1115, 1124, 1829
HpyF3I	CTNAG	9 cut(s)	75, 294, 546, 662, 811, 1201, 1297, 1728, 2357
HpySE526I	ACGT	3 cut(s)	316, 777, 1397
Hsp92I	GRCGYC	1 cut(s)	2061
Hsp92II	CATG	8 cut(s)	139, 1540, 1664, 1993, 2228, 2241, 2326, 2338
HspAI	GCGC	3 cut(s)	163, 872, 1832
Kpn2I	TCCGGA	1 cut(s)	412
Kzo9I	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
LguI	GCTCTTC	1 cut(s)	27
LmnI	GCTCC	6 cut(s)	170, 201, 694, 952, 1492, 2540
Lsp1109I	GCAGC	7 cut(s)	49, 526, 1105, 1738, 1804, 1807, 2287
LweI	GCATC	8 cut(s)	7, 222, 406, 430, 825, 869, 918, 2272
MaeII	ACGT	3 cut(s)	316, 777, 1397
MaeIII	GTNAC	6 cut(s)	181, 915, 1638, 2178, 2340, 2393
MalI	GATC	5 cut(s)	1794, 2127, 2154, 2199, 2355
MboI	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
MfeI	CAATTG	1 cut(s)	1104
MflI	RGATCY	4 cut(s)	1792, 2125, 2197, 2353
MhlI	GDGCHC	1 cut(s)	2220
MlsI	TGGCCA	1 cut(s)	1440
MluNI	TGGCCA	1 cut(s)	1440
MlyI	GAGTC	9 cut(s)	646, 843, 1052, 1067, 1073, 1144, 1414, 2348, 2498
MmeI	TCCRAC	1 cut(s)	2526
Mox20I	TGGCCA	1 cut(s)	1440
Mph1103I	ATGCAT	1 cut(s)	445
MroI	TCCGGA	1 cut(s)	412
MroXI	GAANNNNTTC	3 cut(s)	789, 1261, 1716
MscI	TGGCCA	1 cut(s)	1440
MseI	TTAA	9 cut(s)	87, 450, 501, 630, 641, 671, 2018, 2139, 2415
MslI	CAYNNNNRTG	4 cut(s)	414, 1988, 2005, 2483
Msp20I	TGGCCA	1 cut(s)	1440
MspA1I	CMGCKG	2 cut(s)	517, 1118
MspI	CCGG	3 cut(s)	122, 152, 413
MspR9I	CCNGG	8 cut(s)	122, 123, 153, 570, 621, 1477, 1797, 2037
MunI	CAATTG	1 cut(s)	1104
MvaI	CCWGG	5 cut(s)	570, 621, 1477, 1797, 2037
MvnI	CGCG	1 cut(s)	265
MwoI	GCNNNNNNNGC	7 cut(s)	46, 514, 749, 961, 1115, 1124, 1829
NciI	CCSGG	3 cut(s)	122, 123, 153
NdeII	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
NheI	GCTAGC	1 cut(s)	1813
NlaIII	CATG	8 cut(s)	139, 1540, 1664, 1993, 2228, 2241, 2326, 2338
NlaIV	GGNNCC	7 cut(s)	120, 150, 522, 1481, 2127, 2218, 2536
NmeAIII	GCCGAG	1 cut(s)	1895
NmuCI	GTSAC	3 cut(s)	2178, 2340, 2393
NsiI	ATGCAT	1 cut(s)	445
NspI	RCATGY	1 cut(s)	139
PaeI	GCATGC	1 cut(s)	139
PagI	TCATGA	1 cut(s)	2322
PceI	AGGCCT	1 cut(s)	799
PciSI	GCTCTTC	1 cut(s)	27
PdmI	GAANNNNTTC	3 cut(s)	789, 1261, 1716
PfeI	GAWTC	8 cut(s)	966, 1262, 1421, 1511, 1781, 1948, 1993, 2432
PflMI	CCANNNNNTGG	1 cut(s)	1457
PfoI	TCCNGGA	2 cut(s)	619, 1475
PkrI	GCNGC	7 cut(s)	39, 516, 1120, 1753, 1819, 1822, 2302
PleI	GAGTC	9 cut(s)	646, 843, 1051, 1066, 1073, 1144, 1413, 2347, 2498
PmaCI	CACGTG	1 cut(s)	778
PmlI	CACGTG	1 cut(s)	778
PpsI	GAGTC	9 cut(s)	646, 843, 1051, 1066, 1073, 1144, 1413, 2347, 2498
Ppu21I	YACGTR	1 cut(s)	778
PpuMI	RGGWCCY	1 cut(s)	521
Psp5II	RGGWCCY	1 cut(s)	521
Psp6I	CCWGG	5 cut(s)	568, 619, 1475, 1795, 2035
PspCI	CACGTG	1 cut(s)	778
PspGI	CCWGG	5 cut(s)	568, 619, 1475, 1795, 2035
PspN4I	GGNNCC	7 cut(s)	120, 150, 522, 1481, 2127, 2218, 2536
PspOMI	GGGCCC	1 cut(s)	2216
PspPI	GGNCC	6 cut(s)	118, 349, 521, 1460, 2216, 2217
PspPPI	RGGWCCY	1 cut(s)	521
PsrI	GAACNNNNNNTAC	2 cut(s)	534, 566
PstI	CTGCAG	3 cut(s)	1185, 2104, 2305
PstNI	CAGNNNCTG	1 cut(s)	1751
PsuI	RGATCY	4 cut(s)	1792, 2125, 2197, 2353
PvuII	CAGCTG	1 cut(s)	1118
RsaI	GTAC	3 cut(s)	1026, 1233, 2382
RsaNI	GTAC	3 cut(s)	1025, 1232, 2381
RseI	CAYNNNNRTG	4 cut(s)	414, 1988, 2005, 2483
SapI	GCTCTTC	1 cut(s)	27
SaqAI	TTAA	9 cut(s)	87, 450, 501, 630, 641, 671, 2018, 2139, 2415
SatI	GCNGC	7 cut(s)	38, 515, 1119, 1752, 1818, 1821, 2301
Sau3AI	GATC	5 cut(s)	1792, 2125, 2152, 2197, 2353
Sau96I	GGNCC	6 cut(s)	118, 349, 521, 1460, 2216, 2217
SchI	GAGTC	9 cut(s)	646, 843, 1052, 1067, 1073, 1144, 1414, 2348, 2498
ScrFI	CCNGG	8 cut(s)	122, 123, 153, 570, 621, 1477, 1797, 2037
SduI	GDGCHC	1 cut(s)	2220
SfaNI	GCATC	8 cut(s)	7, 222, 406, 430, 825, 869, 918, 2272
SfcI	CTRYAG	5 cut(s)	509, 1181, 1968, 2100, 2301
SinI	GGWCC	2 cut(s)	118, 521
SmaI	CCCGGG	1 cut(s)	123
SmiI	ATTTAAAT	1 cut(s)	672
SmiMI	CAYNNNNRTG	4 cut(s)	414, 1988, 2005, 2483
SmlI	CTYRAG	2 cut(s)	734, 1166
SmoI	CTYRAG	2 cut(s)	734, 1166
SphI	GCATGC	1 cut(s)	139
SseBI	AGGCCT	1 cut(s)	799
SsiI	CCGC	6 cut(s)	265, 277, 401, 517, 1332, 1483
StuI	AGGCCT	1 cut(s)	799
StyD4I	CCNGG	8 cut(s)	120, 121, 151, 568, 619, 1475, 1795, 2035
SwaI	ATTTAAAT	1 cut(s)	672
TaiI	ACGT	3 cut(s)	319, 780, 1400
TaqI	TCGA	7 cut(s)	131, 839, 877, 1571, 1803, 2295, 2430
TatI	WGTACW	2 cut(s)	1024, 2380
TfiI	GAWTC	8 cut(s)	966, 1262, 1421, 1511, 1781, 1948, 1993, 2432
Tru1I	TTAA	9 cut(s)	87, 450, 501, 630, 641, 671, 2018, 2139, 2415
Tru9I	TTAA	9 cut(s)	87, 450, 501, 630, 641, 671, 2018, 2139, 2415
TscAI	CASTG	6 cut(s)	111, 326, 1129, 2212, 2398, 2497
TseFI	GTSAC	3 cut(s)	2178, 2340, 2393
TseI	GCWGC	7 cut(s)	37, 514, 1118, 1751, 1817, 1820, 2300
Tsp45I	GTSAC	3 cut(s)	2178, 2340, 2393
TspDTI	ATGAA	7 cut(s)	398, 1566, 1611, 1649, 1773, 2238, 2339
TspGWI	ACGGA	3 cut(s)	33, 214, 702
TspMI	CCCGGG	1 cut(s)	121
TspRI	CASTG	6 cut(s)	111, 326, 1129, 2212, 2398, 2497
Van91I	CCANNNNNTGG	1 cut(s)	1457
VpaK11BI	GGWCC	2 cut(s)	118, 521
XapI	RAATTY	1 cut(s)	259
XbaI	TCTAGA	1 cut(s)	580
XceI	RCATGY	1 cut(s)	139
XmaI	CCCGGG	1 cut(s)	121
XmnI	GAANNNNTTC	3 cut(s)	789, 1261, 1716
Zsp2I	ATGCAT	1 cut(s)	445

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.