RchiOBHm_Chr6g0286081

Domain found in a variety of signalling proteins, always encircled by uDENN and dDENN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006052
Physical Location & Seq
Forward (+)
49372308 .. 49380338
8031 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25662

Sequence Viewer

Length: 2742 bp
ATGAGCTTAGTGTTTGAAATGGATGCAAAAGAAGATGCTGAAGGACACGAAGAAGGGGGTTTGTCCCCTATTCAGGTTATATCTGAGGAGGCATGCACAGTGGCAGGAGAAGCCCCCAATAACTTGGCCAGTCCGAATATGCCGCCATTGGCACCGGGGAATGGGCATAGGCGGACCCAAAGTGATGTTTTGACCACCAAACACAGGCGCAGTCATAGCTTTCAGAGATTGAAAAACCAAATGCAGAAGGCTTGGAGATGGGGGACCAATCATGATTCCCGGCTGGCTTTCAATCCTGAGGTTTTGGCAAACCAAAAACGCCAGTGGTATCAGCTTCACTCCAAATCAAAGGGTCATGTAAATTATCAGGAGCCAACTTCACTCTTTGAGCATTTTATAATTGCGGGGCTTCATCCAGATACTAATCTTAAAAATGCCGAGGCAGCATTCATTAAAAGAAAGAGATGGGAGACGGACATGATAAGCTATGGAATTACCGACCTTAAATTACTGCAGCAGAAGGGACCTCCAGCCCCAATATTAGAACCTCAGGTTGAATGCATGACTGCTTATGAAGCTTCCTGCAAACCTCTTTCCTGTTTTGTTTTTGGTGTACTTTTTAAATATCCTCCCGGGAAGAGGCTAGCAATGCGTTTGAAAGATTTAGCTTCCTTTTGTTTTCCTGGAGGTGTTAAGGCACGATTAATGGAGAAGACTCCATCACTAAGTGACCTTAATCAAATTGTGTATGGACAGGTACGGCATCTCACCAAGATCTATATGTTTCATTTTGAAGTTACACAACTTATTGTCTGTTGGATTGGTACCAGTAACAAGATTGTAGTAGGGTGTGAGCTATTATCAAGTTGTCCATTTAAAACAGTCCAGTATGAGCATTTAGGCAAAGATGATTTAGCATTTATCTTTTCACTCAAGGTAGCAGACAATGCAACACTTTATGGTATTTGCCTACATGTGTTGGAAATAGTTCAGAGGCCTCCTGCTATCTTAGGCATCACATCACCTCTTCCTCATTCATCTGAAGGACTCTTCCGTTTTTTGGTTTCTGCACCTCGGTGCTACTGTCTGTTATCCAAATATCCTTTTTTTGAGTTACATTACGAGATGTTGAACAGAAAAACATTTATAAAATTCAAAGCAATGCACCATCTTTTACTTGGATGCAGTATCATTCAACAGGAGCGCCTGAGTCGAATAACACAACTTGCTAATGGAATGAGCCTCAATGACCATGTCCCTTCAATGCCCAAAATAGATGATGAAATGAATAATAATGAGGAGTCCCCTGAGACAGAGTCTTTTGATGATTGGATGAATTCTGCAATCCCTGTTGACAGTGCAATAGCCCGTACAGCTGTTGCTGCAGGAATTACACCTGATGATGAGACTTCAACTTCTTCACTCAAGATATGGGAACCACAGTCCCCGGAAAGTGCTTCTGCTAGTGAGGCTCCAGATTTTATTCAAGTACGCTATTTAGATAAGGATGATAGAAAGGATTCACAATGTTTTGATGACTGTGGTTCCTCAGAAACTTCAGAAAGAATATATGGAAGTCACACAAATGGCCATACTTCACGCGAGAGTCTTGGGAGTTCTGAATCTCTATTTAGAAATTGTCATACTTCTCCAGAAGTTGGGACATCTTTCTCCTCCAGGAATCGCACATTGGAGCGTTTTGGGAGTTCTGAATCTCTATTCAGTCCAGCTAGAAGCATGTCAGAGGATGATGATGATGACCTTTCCTCAACTTGTGAGCAAGATTTTAGCGATGACTTGATAATGGCATGGGCTAGGGAGAATAAAAACGATTTGCTACAGATTGTATGTGGTTTTCATGCTCTTCCTCTTCCCGAACGAGGAAGTGAGTTAGTTTTCCAACCTCTTGAACATCTACAGGCAATCCAGTATAATCGACCTCCAATTGCTGCCCTTGGTTTTGATGAAAAAAATTTAGATTCACCTGAAGATCCGGGGGTTGATGCAAAGTTGGCTGCTGCTGAGGAAGCGCTTTCACTATCACTGTGGACAACTGCTACAATTTGCCGAGTTCTCTCTGTTGAAAGTATTTTGGCATTGGTTGCGGGAGTACTACTGGAAAAGCAAGTGATATTAGTATGCCCTAATCTGGGTGTTCTATCAGCTACAGTGTTATCTCTCATACCCATGATTCGTCCATTTCAATGGCAGAGTTTAATGCTTCCTGTTCTACCAGGGAAAATGCTGGATTTTCTTGATGCCCCAGTTCCCTTCATTATTGGGATAGAACAAGTTCCTGCAGATCTAAGGAAGAAAACATCAGATCCTGTTGTAGTTAACATGCTCATGGATCAGGTGAAACTGCCTCATCACTTGCCAACATTACCACGATATAGGGATCTTGCCCTTGAACTCGCGCCAATCCATTCTAAACTGTCACTCGAAGGTTCATTTGCTAAAAAGCATCCTGTATATAGGTGCAACGAAGTGCAGGCTGAAGCTGCAGCCCAGTTTTTGAAAGTCATGAAGAACTACATTGAGTCACTTTGTTTAGAGCTAAGGTCTTACACCATAACAAGTGTACAGTCAAATAATGACAGGGTTTCGTTACTTCTCAAAGATAGCTTCATTGATTCCTTTCCTAGTAAGGACCGGTCATTTATCAAGTTGTTGGTAGACACACAAATGTTCACAGTTCTATCCGACTCTCGTCTGTCAAGCTTAGAGAATGGCAGCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DENN dDENN uDENN

Protein Analysis

913

Amino Acids

102.16

Weight (kDa)

5.66

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DENN PF02141 687 - 780 9.1e-22 DENN (AEX-3) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 398, 1148
Acc65I GGTACC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 151, 824
AccB7I CCANNNNNTGG 1 cut(s) 1658
AccI GTMKAC 1 cut(s) 2676
AccII CGCG 2 cut(s) 1602, 2417
AciI CCGC 4 cut(s) 143, 172, 404, 2105
AclWI GGATC 4 cut(s) 1985, 2318, 2358, 2406
AcoI YGGCCR 2 cut(s) 126, 1588
AcsI RAATTY 3 cut(s) 1151, 1336, 1972
AcuI CTGAAG 5 cut(s) 60, 1062, 1542, 2007, 2517
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 7 cut(s) 615, 759, 826, 1372, 1491, 2112, 2583
AfeI AGCGCT 1 cut(s) 2031
AfiI CCNNNNNNNGG 9 cut(s) 73, 161, 204, 639, 1060, 1658, 1880, 2149, 2150
AflIII ACRYGT 1 cut(s) 973
AgeI ACCGGT 1 cut(s) 2652
AjnI CCWGG 3 cut(s) 682, 1676, 2233
AjuI GAANNNNNNNTTGG 2 cut(s) 1673, 1705
AleI CACNNNNGTG 1 cut(s) 1075
Alw26I GTCTC 3 cut(s) 464, 1304, 1400
AlwI GGATC 4 cut(s) 1985, 2318, 2358, 2406
AlwNI CAGNNNCTG 1 cut(s) 2327
Ama87I CYCGRG 1 cut(s) 632
Aor51HI AGCGCT 1 cut(s) 2031
AoxI GGCC 3 cut(s) 126, 995, 1588
ApeKI GCWGC 9 cut(s) 443, 514, 1382, 1949, 2015, 2018, 2501, 2504, 2733
ApoI RAATTY 3 cut(s) 1151, 1336, 1972
AseI ATTAAT 1 cut(s) 704
AsiGI ACCGGT 1 cut(s) 2652
Asp700I GAANNNNTTC 3 cut(s) 987, 1519, 2292
Asp718I GGTACC 1 cut(s) 824
AspLEI GCGC 4 cut(s) 210, 1206, 2032, 2419
AspS9I GGNCC 4 cut(s) 174, 264, 524, 2650
AsuC2I CCSGG 6 cut(s) 156, 280, 633, 634, 1448, 1995
AsuHPI GGTGA 4 cut(s) 760, 1014, 1974, 2368
AsuNHI GCTAGC 1 cut(s) 643
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 4 cut(s) 174, 264, 524, 2650
AxyI CCTNAGG 2 cut(s) 297, 549
BalI TGGCCA 2 cut(s) 128, 1590
BanI GGYRCC 2 cut(s) 151, 824
BbsI GAAGAC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 2022
BbvI GCAGC 8 cut(s) 455, 526, 1369, 1936, 2002, 2005, 2488, 2516
BccI CCATC 4 cut(s) 252, 459, 727, 1176
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 3 cut(s) 684, 1678, 2235
BcnI CCSGG 6 cut(s) 156, 280, 633, 634, 1448, 1995
BcoDI GTCTC 3 cut(s) 464, 1304, 1400
BfaI CTAG 5 cut(s) 644, 1464, 1731, 1815, 2643
BfmI CTRYAG 7 cut(s) 512, 1383, 1838, 1916, 2166, 2298, 2502
BfoI RGCGCY 2 cut(s) 1207, 2033
BglII AGATCT 2 cut(s) 774, 2302
BmcAI AGTACT 1 cut(s) 2112
Bme1390I CCNGG 9 cut(s) 156, 280, 633, 634, 684, 1448, 1678, 1995, 2235
Bme18I GGWCC 4 cut(s) 174, 264, 524, 2650
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 4 cut(s) 174, 264, 524, 2650
BmiI GGNNCC 9 cut(s) 153, 176, 265, 372, 525, 826, 1437, 1473, 1546
BmrFI CCNGG 9 cut(s) 156, 280, 633, 634, 684, 1448, 1678, 1995, 2235
BmrI ACTGGG 2 cut(s) 2258, 2503
BmsI GCATC 8 cut(s) 13, 25, 772, 1023, 1172, 1993, 2248, 2473
BmtI GCTAGC 1 cut(s) 647
BmuI ACTGGG 2 cut(s) 2258, 2503
BoxI GACNNNNGTC 1 cut(s) 2709
BpiI GAAGAC 1 cut(s) 719
BpmI CTGGAG 5 cut(s) 513, 705, 1458, 1635, 1660
Bpu10I CCTNAGC 2 cut(s) 2022, 2558
BpuEI CTTGAG 2 cut(s) 917, 1409
BpuMI CCSGG 6 cut(s) 156, 280, 633, 634, 1448, 1995
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 8 cut(s) 155, 438, 632, 1073, 1446, 1954, 1994, 2234
BsaWI WCCGGW 1 cut(s) 2652
Bsc4I CCNNNNNNNGG 9 cut(s) 73, 161, 204, 639, 1060, 1658, 1880, 2149, 2150
Bse118I RCCGGY 1 cut(s) 2652
Bse1I ACTGG 8 cut(s) 129, 322, 828, 886, 1927, 2121, 2264, 2509
Bse21I CCTNAGG 2 cut(s) 297, 549
Bse3DI GCAATG 2 cut(s) 654, 1167
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 3 cut(s) 684, 1678, 2235
BseDI CCNNGG 8 cut(s) 155, 438, 632, 1073, 1446, 1954, 1994, 2234
BseGI GGATG 7 cut(s) 28, 412, 1187, 1338, 1513, 1753, 2464
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 9 cut(s) 73, 161, 204, 639, 1060, 1658, 1880, 2149, 2150
BseMI GCAATG 2 cut(s) 654, 1167
BseMII CTCAG 7 cut(s) 75, 288, 563, 1199, 1299, 1563, 2013
BseNI ACTGG 8 cut(s) 129, 322, 828, 886, 1927, 2121, 2264, 2509
BseRI GAGGAG 3 cut(s) 101, 1313, 1663
BseXI GCAGC 8 cut(s) 455, 526, 1369, 1936, 2002, 2005, 2488, 2516
BsgI GTGCAG 2 cut(s) 1053, 2510
Bsh1236I CGCG 2 cut(s) 1602, 2417
BshFI GGCC 3 cut(s) 128, 997, 1590
BshNI GGYRCC 2 cut(s) 151, 824
BshTI ACCGGT 1 cut(s) 2652
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 6 cut(s) 155, 280, 633, 1448, 1994, 2653
BslFI GGGAC 7 cut(s) 49, 277, 537, 1241, 1288, 1429, 1675
BslI CCNNNNNNNGG 9 cut(s) 73, 161, 204, 639, 1060, 1658, 1880, 2149, 2150
BsmAI GTCTC 3 cut(s) 464, 1304, 1400
BsmBI CGTCTC 1 cut(s) 464
BsmFI GGGAC 7 cut(s) 49, 277, 537, 1241, 1288, 1429, 1675
BsmI GAATGC 2 cut(s) 446, 563
BsnI GGCC 3 cut(s) 128, 997, 1590
BsoBI CYCGRG 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 2581
Bsp143I GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
BspACI CCGC 4 cut(s) 143, 172, 404, 2105
BspANI GGCC 3 cut(s) 128, 997, 1590
BspCNI CTCAG 7 cut(s) 76, 289, 562, 1200, 1300, 1562, 2014
BspFNI CGCG 2 cut(s) 1602, 2417
BspHI TCATGA 2 cut(s) 271, 2523
BspLI GGNNCC 9 cut(s) 153, 176, 265, 372, 525, 826, 1437, 1473, 1546
BspMAI CTGCAG 4 cut(s) 516, 1387, 2302, 2506
BspOI GCTAGC 1 cut(s) 647
BspPI GGATC 4 cut(s) 1985, 2318, 2358, 2406
BspQI GCTCTTC 1 cut(s) 1869
BspT107I GGYRCC 2 cut(s) 151, 824
BsrDI GCAATG 2 cut(s) 654, 1167
BsrFI RCCGGY 1 cut(s) 2652
BsrGI TGTACA 1 cut(s) 2581
BsrI ACTGG 8 cut(s) 129, 322, 828, 886, 1927, 2121, 2264, 2509
BssAI RCCGGY 1 cut(s) 2652
BssECI CCNNGG 8 cut(s) 155, 438, 632, 1073, 1446, 1954, 1994, 2234
BssMI GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
BssT1I CCWWGG 1 cut(s) 1954
Bst2UI CCWGG 3 cut(s) 684, 1678, 2235
Bst6I CTCTTC 5 cut(s) 632, 1032, 1055, 1869, 1875
BstAPI GCANNNNNTGC 2 cut(s) 947, 2102
BstAUI TGTACA 1 cut(s) 2581
BstC8I GCNNGC 4 cut(s) 94, 285, 645, 2493
BstF5I GGATG 7 cut(s) 28, 412, 1187, 1338, 1513, 1753, 2464
BstFNI CGCG 2 cut(s) 1602, 2417
BstH2I RGCGCY 2 cut(s) 1207, 2033
BstHHI GCGC 4 cut(s) 210, 1206, 2032, 2419
BstKTI GATC 6 cut(s) 777, 1993, 2305, 2326, 2353, 2401
BstMAI GTCTC 3 cut(s) 464, 1304, 1400
BstMBI GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
BstNI CCWGG 3 cut(s) 684, 1678, 2235
BstNSI RCATGY 4 cut(s) 96, 977, 1741, 2344
BstPAI GACNNNNGTC 1 cut(s) 2709
BstSCI CCNGG 9 cut(s) 154, 278, 631, 632, 682, 1446, 1676, 1993, 2233
BstSFI CTRYAG 7 cut(s) 512, 1383, 1838, 1916, 2166, 2298, 2502
BstUI CGCG 2 cut(s) 1602, 2417
BstV1I GCAGC 8 cut(s) 455, 526, 1369, 1936, 2002, 2005, 2488, 2516
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 5 cut(s) 774, 1990, 2302, 2323, 2398
BstXI CCANNNNNNTGG 2 cut(s) 124, 2205
BstYI RGATCY 5 cut(s) 774, 1990, 2302, 2323, 2398
Bsu36I CCTNAGG 2 cut(s) 297, 549
BsuRI GGCC 3 cut(s) 128, 997, 1590
BtgZI GCGATG 1 cut(s) 1806
BtsCI GGATG 7 cut(s) 28, 412, 1187, 1338, 1513, 1753, 2464
BtsIMutI CAGTG 5 cut(s) 105, 329, 1363, 2042, 2175
Cac8I GCNNGC 4 cut(s) 94, 285, 645, 2493
CaiI CAGNNNCTG 1 cut(s) 2327
CciI TCATGA 2 cut(s) 271, 2523
CfoI GCGC 4 cut(s) 210, 1206, 2032, 2419
Cfr10I RCCGGY 1 cut(s) 2652
Cfr13I GGNCC 4 cut(s) 174, 264, 524, 2650
Cfr9I CCCGGG 1 cut(s) 632
Csp6I GTAC 7 cut(s) 614, 758, 825, 1371, 1490, 2111, 2582
CspAI ACCGGT 1 cut(s) 2652
CviQI GTAC 7 cut(s) 614, 758, 825, 1371, 1490, 2111, 2582
DpnI GATC 6 cut(s) 776, 1992, 2304, 2325, 2352, 2400
DpnII GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
DraI TTTAAA 2 cut(s) 622, 877
DraIII CACNNNGTG 1 cut(s) 728
EaeI YGGCCR 2 cut(s) 126, 1588
Eam1104I CTCTTC 5 cut(s) 632, 1032, 1055, 1869, 1875
EarI CTCTTC 5 cut(s) 632, 1032, 1055, 1869, 1875
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 1954
Eco147I AGGCCT 1 cut(s) 997
Eco47I GGWCC 4 cut(s) 174, 264, 524, 2650
Eco47III AGCGCT 1 cut(s) 2031
Eco57I CTGAAG 5 cut(s) 60, 1062, 1542, 2007, 2517
Eco81I CCTNAGG 2 cut(s) 297, 549
Eco88I CYCGRG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 3 cut(s) 682, 1676, 2233
EcoT14I CCWWGG 1 cut(s) 1954
EcoT22I ATGCAT 1 cut(s) 563
ErhI CCWWGG 1 cut(s) 1954
Esp3I CGTCTC 1 cut(s) 464
FaqI GGGAC 7 cut(s) 49, 277, 537, 1241, 1288, 1429, 1675
FauI CCCGC 2 cut(s) 397, 2098
FblI GTMKAC 1 cut(s) 2676
FokI GGATG 7 cut(s) 35, 399, 1194, 1345, 1520, 1760, 2451
FspBI CTAG 5 cut(s) 644, 1464, 1731, 1815, 2643
GlaI GCGC 4 cut(s) 209, 1205, 2031, 2418
GsuI CTGGAG 5 cut(s) 513, 705, 1458, 1635, 1660
HaeII RGCGCY 2 cut(s) 1207, 2033
HaeIII GGCC 3 cut(s) 128, 997, 1590
HapII CCGG 6 cut(s) 155, 280, 633, 1448, 1994, 2653
HhaI GCGC 4 cut(s) 210, 1206, 2032, 2419
Hin6I GCGC 4 cut(s) 208, 1204, 2030, 2417
HinP1I GCGC 4 cut(s) 208, 1204, 2030, 2417
HincII GTYRAC 2 cut(s) 1354, 2338
HindII GTYRAC 2 cut(s) 1354, 2338
HindIII AAGCTT 2 cut(s) 576, 2719
HpaI GTTAAC 1 cut(s) 2338
HpaII CCGG 6 cut(s) 155, 280, 633, 1448, 1994, 2653
HphI GGTGA 4 cut(s) 760, 1014, 1974, 2368
Hpy166II GTNNAC 7 cut(s) 614, 1354, 2049, 2338, 2582, 2677, 2691
Hpy8I GTNNAC 7 cut(s) 614, 1354, 2049, 2338, 2582, 2677, 2691
HpyAV CCTTC 8 cut(s) 35, 47, 241, 514, 1037, 1269, 2281, 2438
HspAI GCGC 4 cut(s) 208, 1204, 2030, 2417
KpnI GGTACC 1 cut(s) 828
KspAI GTTAAC 1 cut(s) 2338
Kzo9I GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
LguI GCTCTTC 1 cut(s) 1869
LmnI GCTCC 4 cut(s) 370, 1201, 1477, 1693
Lsp1109I GCAGC 8 cut(s) 455, 526, 1369, 1936, 2002, 2005, 2488, 2516
LweI GCATC 8 cut(s) 13, 25, 772, 1023, 1172, 1993, 2248, 2473
MaeI CTAG 5 cut(s) 644, 1464, 1731, 1815, 2643
MaeIII GTNAC 8 cut(s) 728, 796, 830, 1113, 1577, 2436, 2541, 2607
MalI GATC 6 cut(s) 776, 1992, 2304, 2325, 2352, 2400
MboI GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
MfeI CAATTG 1 cut(s) 1944
MflI RGATCY 5 cut(s) 774, 1990, 2302, 2323, 2398
MlsI TGGCCA 2 cut(s) 128, 1590
MluNI TGGCCA 2 cut(s) 128, 1590
MlyI GAGTC 8 cut(s) 709, 1041, 1219, 1310, 1325, 1615, 2549, 2699
MmeI TCCRAC 4 cut(s) 797, 960, 1924, 2727
Mox20I TGGCCA 2 cut(s) 128, 1590
Mph1103I ATGCAT 1 cut(s) 563
MroXI GAANNNNTTC 3 cut(s) 987, 1519, 2292
MscI TGGCCA 2 cut(s) 128, 1590
MslI CAYNNNNRTG 6 cut(s) 1075, 1584, 2186, 2203, 2345, 2684
Msp20I TGGCCA 2 cut(s) 128, 1590
MspA1I CMGCKG 1 cut(s) 1376
MspI CCGG 6 cut(s) 155, 280, 633, 1448, 1994, 2653
MspR9I CCNGG 9 cut(s) 156, 280, 633, 634, 684, 1448, 1678, 1995, 2235
MunI CAATTG 1 cut(s) 1944
Mva1269I GAATGC 2 cut(s) 446, 563
MvaI CCWGG 3 cut(s) 684, 1678, 2235
MvnI CGCG 2 cut(s) 1602, 2417
NciI CCSGG 6 cut(s) 156, 280, 633, 634, 1448, 1995
NdeII GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
NheI GCTAGC 1 cut(s) 643
NlaIV GGNNCC 9 cut(s) 153, 176, 265, 372, 525, 826, 1437, 1473, 1546
NmeAIII GCCGAG 2 cut(s) 463, 2093
NmuCI GTSAC 4 cut(s) 728, 1577, 2436, 2541
NsiI ATGCAT 1 cut(s) 563
NspI RCATGY 4 cut(s) 96, 977, 1741, 2344
OliI CACNNNNGTG 1 cut(s) 1075
PaeI GCATGC 1 cut(s) 96
PagI TCATGA 2 cut(s) 271, 2523
PceI AGGCCT 1 cut(s) 997
PciI ACATGT 1 cut(s) 973
PciSI GCTCTTC 1 cut(s) 1869
PctI GAATGC 2 cut(s) 446, 563
PdmI GAANNNNTTC 3 cut(s) 987, 1519, 2292
PfeI GAWTC 8 cut(s) 275, 1520, 1622, 1681, 1712, 1979, 2191, 2633
PflFI GACNNNGTC 2 cut(s) 1253, 1315
PflMI CCANNNNNTGG 1 cut(s) 1658
PfoI TCCNGGA 2 cut(s) 682, 1676
PinAI ACCGGT 1 cut(s) 2652
PleI GAGTC 8 cut(s) 709, 1041, 1218, 1309, 1324, 1614, 2548, 2699
PpsI GAGTC 8 cut(s) 709, 1041, 1218, 1309, 1324, 1614, 2548, 2699
PpuMI RGGWCCY 1 cut(s) 524
PscI ACATGT 1 cut(s) 973
PshAI GACNNNNGTC 1 cut(s) 2709
PshBI ATTAAT 1 cut(s) 704
PsiI TTATAA 2 cut(s) 398, 1148
Psp5II RGGWCCY 1 cut(s) 524
Psp6I CCWGG 3 cut(s) 682, 1676, 2233
PspGI CCWGG 3 cut(s) 682, 1676, 2233
PspN4I GGNNCC 9 cut(s) 153, 176, 265, 372, 525, 826, 1437, 1473, 1546
PspPI GGNCC 4 cut(s) 174, 264, 524, 2650
PspPPI RGGWCCY 1 cut(s) 524
PstI CTGCAG 4 cut(s) 516, 1387, 2302, 2506
PstNI CAGNNNCTG 1 cut(s) 2327
PsuI RGATCY 5 cut(s) 774, 1990, 2302, 2323, 2398
PsyI GACNNNGTC 2 cut(s) 1253, 1315
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 7 cut(s) 615, 759, 826, 1372, 1491, 2112, 2583
RsaNI GTAC 7 cut(s) 614, 758, 825, 1371, 1490, 2111, 2582
RseI CAYNNNNRTG 6 cut(s) 1075, 1584, 2186, 2203, 2345, 2684
SapI GCTCTTC 1 cut(s) 1869
Sau3AI GATC 6 cut(s) 774, 1990, 2302, 2323, 2350, 2398
Sau96I GGNCC 4 cut(s) 174, 264, 524, 2650
ScaI AGTACT 1 cut(s) 2112
SchI GAGTC 8 cut(s) 709, 1041, 1219, 1310, 1325, 1615, 2549, 2699
ScrFI CCNGG 9 cut(s) 156, 280, 633, 634, 684, 1448, 1678, 1995, 2235
SfaNI GCATC 8 cut(s) 13, 25, 772, 1023, 1172, 1993, 2248, 2473
SfcI CTRYAG 7 cut(s) 512, 1383, 1838, 1916, 2166, 2298, 2502
SinI GGWCC 4 cut(s) 174, 264, 524, 2650
SmaI CCCGGG 1 cut(s) 634
SmiMI CAYNNNNRTG 6 cut(s) 1075, 1584, 2186, 2203, 2345, 2684
SmlI CTYRAG 2 cut(s) 932, 1424
SmoI CTYRAG 2 cut(s) 932, 1424
SphI GCATGC 1 cut(s) 96
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 4 cut(s) 143, 172, 404, 2105
SspI AATATT 1 cut(s) 540
SspMI CTAG 5 cut(s) 644, 1464, 1731, 1815, 2643
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 9 cut(s) 154, 278, 631, 632, 682, 1446, 1676, 1993, 2233
StyI CCWWGG 1 cut(s) 1954
TaqI TCGA 3 cut(s) 1213, 1936, 2442
TatI WGTACW 3 cut(s) 613, 2110, 2581
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 8 cut(s) 275, 1520, 1622, 1681, 1712, 1979, 2191, 2633
TscAI CASTG 5 cut(s) 105, 329, 1363, 2049, 2175
TseFI GTSAC 4 cut(s) 728, 1577, 2436, 2541
TseI GCWGC 9 cut(s) 443, 514, 1382, 1949, 2015, 2018, 2501, 2504, 2733
Tsp45I GTSAC 4 cut(s) 728, 1577, 2436, 2541
TspGWI ACGGA 2 cut(s) 488, 1043
TspMI CCCGGG 1 cut(s) 632
TspRI CASTG 5 cut(s) 105, 329, 1363, 2049, 2175
Tth111I GACNNNGTC 2 cut(s) 1253, 1315
Van91I CCANNNNNTGG 1 cut(s) 1658
VpaK11BI GGWCC 4 cut(s) 174, 264, 524, 2650
VspI ATTAAT 1 cut(s) 704
XapI RAATTY 3 cut(s) 1151, 1336, 1972
XceI RCATGY 4 cut(s) 96, 977, 1741, 2344
XcmI CCANNNNNNNNNTGG 1 cut(s) 1175
XmaI CCCGGG 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 2676
XmnI GAANNNNTTC 3 cut(s) 987, 1519, 2292
XspI CTAG 5 cut(s) 644, 1464, 1731, 1815, 2643
ZrmI AGTACT 1 cut(s) 2112
Zsp2I ATGCAT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.