Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005873

Physical Location & Seq

Genomic Coordinates Reverse (-)

37149523 .. 37152328

Gene Structure 2806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1240800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1494 bp

ATGAAGATGGAGCAAAATTCAACCCTCTTCATCATTTTCCTCCTCTCCGTAATCCACAGCTTCGTGTTCGTTCATGCACTCCGCGGCGCGAGCCCGGAGAAAGCCTTGAAGCTCAAGCTAATCCACAGGTACTCTCCACATTATAATGGCCTGCATGGGGATGAGAAGCCTAAAAACCAGCAGGAGCTCCTCAGGTTGCTCCACCGCCACGACATTGTCCGCCATCAAATGATATCGTACAGAAGGCAACAACAACAAGAAGAAAGCCTTCTTGATGCTGAAGAGGTAATATTAAACAGCAGCCGGATCGCGGCGAGGCGGATGGCTTGGGAAAAAAGAGGCTCCATGGCAATGCCGATAAGTTCGGGTTCGGATTACGGTTGGGGGCAGTACCTGGTTAAAATTAAAATCGGAACGCCGGCACAAAAGTTCTTGTTAGTCGCGGATACCGGCAGTGACTTGACGTGGATAAACTGCAGATACCGTTGCAGAAACCGGTGCGAGAAGCACCAAGGAAGGTTGCAACACAAGAGGGTTTTCCATGCAGAACTTTCTTCTTCCTTTAAAACTGTGCCGTGTTCTTCGAAATTGTGCAAGGTTGGTCTGTGGACTATGTTTTCTCTCCAACAGTGCTCCACCCCATCGAGCCCTTGCAGATATGATTACTCGTACATAGAGGGGGCTCATGCATTTGGGCTGTTTGCCAATGAGACTGTAAGAGCAACCCTGGCAAGTGGCACGAGAACCAAACTGGAAAACGTGATAGTTGGTTGCACAGACCACATCAAGGGCAGTGGAGGCATTAGACATGGTGATGGTATATTGGGGTTGGGCTTTGGCAGGAACTCATTCACTACCAAGGCTTCCCTACACTTTGGTGGCAAGTTTTCTTATTGCCTGGTTGATCAGCAAAGCCCCAAACATGTCTCAAATTTTCTCACATTTGGTGGCCACAAAGCAGCTAGATTGCAGAAACAAATGAACTACACAAAGCTGGTTATCGGTGACTCCGAGGATAAGGGGTCGTTCTACGGCGTAAATGTGCAGGGAATCTCAGATGGGGGGAAAATGTTAGATATACCACCTCGTGTGTGGGACGAAAATCTTAAAGGTGGAACCATTGTTGATTCAGGCACAAGCCTCACATTTCTGAAAGTGCCGGCATATAAAGCTGTCATGAAAGTAATGACAATGGCTTTGTCGAAAGTGAAAAAATTGTCACCAGATGATGACCCTTTTGACTTTTGCTTCAGTTCCAAGGGATTCAACAGGTCTTTAGTGCCAAAATTCGCAATTCACTTTGCAGATGGAGCTATATTTGAGCCACCAGTGAAGAGCTATGTTCTTGAGGTTGCTGACGGGAAGATGTGCCTAGGGTTTGTGCCAACTAATGTTGGTCCCTCTGTTATTGGCAACATAATGCAGCAAAACCATTTGTGGGAATTTGACTTAGGGAGGAGGACACTGGGTTTTGCTCCCTCCACCTGCACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009743 GO:0009744 GO:0009888 GO:0009987 GO:0010019 GO:0010033 GO:0010073 GO:0010074 GO:0010492 GO:0016787 GO:0019538 GO:0019827 GO:0023052 GO:0030163 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048507 GO:0048511 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070001 GO:0070011 GO:0071704 GO:0098727 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

498

Amino Acids

55.52

Weight (kDa)

9.45

Isoelectric Point (pI)

43.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	130 - 493	1.8e-20	Eukaryotic aspartyl protease
TAXi_N	PF14543	131 - 317	3.3e-43	Xylanase inhibitor N-terminal
TAXi_C	PF14541	344 - 492	5e-34	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12700 AT3G12700 AT3G12700
fragaria_vesca	FvH4_3g13820 FvH4_5g37920
malus_domestica	MD05G1240800.v1.1 MD10G1219200.v1.1
prunus_persica	Prupe.4G124800_v2.0.a1
pyrus_communis	pycom05g21700 pycom10g18470
rosa_chinensis	RchiOBHm_Chr5g0023001 RchiOBHm_Chr7g0239861
rosa_laevigata	RLG00000000724 RLG00000032688
rosa_roxburghii	Rroxscaffold_1G00056340 Rroxscaffold_3G00220750
rosa_rugosa	Rorug05G0076400 Rorug07G0330600
rosa_samantha	Rh5AG162600 Rh5BG161800 Rh5CG176500 Rh5DG163200 Rh7AG484100 Rh7BG457200 Rh7CG501600 Rh7DG469800
rosa_wichuraiana	Rw5G014650 Rw7G040610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	144
AccII	CGCG	4 cut(s)	84, 89, 311, 443
AciI	CCGC	7 cut(s)	82, 84, 205, 220, 311, 319, 443
AclWI	GGATC	1 cut(s)	314
AcoI	YGGCCR	1 cut(s)	949
AcsI	RAATTY	4 cut(s)	16, 931, 1286, 1442
AcuI	CTGAAG	2 cut(s)	300, 1234
AdeI	CACNNNGTG	1 cut(s)	1088
AfaI	GTAC	4 cut(s)	131, 239, 392, 671
AfiI	CCNNNNNNNGG	4 cut(s)	157, 310, 787, 1438
AflIII	ACRYGT	1 cut(s)	922
AgeI	ACCGGT	1 cut(s)	495
AgsI	TTSAA	3 cut(s)	21, 109, 1267
AjiI	CACGTC	1 cut(s)	465
AjnI	CCWGG	3 cut(s)	393, 726, 897
AleI	CACNNNNGTG	1 cut(s)	876
AluBI	AGCT	9 cut(s)	60, 112, 118, 187, 962, 994, 1172, 1313, 1338
AluI	AGCT	9 cut(s)	60, 112, 118, 187, 962, 994, 1172, 1313, 1338
Alw21I	GWGCWC	2 cut(s)	189, 635
Alw26I	GTCTC	2 cut(s)	704, 931
AlwI	GGATC	1 cut(s)	314
AlwNI	CAGNNNCTG	1 cut(s)	394
AoxI	GGCC	2 cut(s)	148, 949
ApeKI	GCWGC	3 cut(s)	300, 959, 1423
ApoI	RAATTY	4 cut(s)	16, 931, 1286, 1442
ArsI	GACNNNNNNTTYG	2 cut(s)	1180, 1212
AsiGI	ACCGGT	1 cut(s)	495
Asp700I	GAANNNNTTC	2 cut(s)	267, 848
AspA2I	CCTAGG	1 cut(s)	1372
AspLEI	GCGC	1 cut(s)	89
AspS9I	GGNCC	1 cut(s)	1397
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	3 cut(s)	824, 1016, 1212
AsuII	TTCGAA	1 cut(s)	584
AvaII	GGWCC	1 cut(s)	1397
AvrII	CCTAGG	1 cut(s)	1372
AxyI	CCTNAGG	1 cut(s)	191
BalI	TGGCCA	1 cut(s)	951
BanII	GRGCYC	4 cut(s)	95, 189, 650, 685
BauI	CACGAG	2 cut(s)	739, 1086
Bbv12I	GWGCWC	2 cut(s)	189, 635
BbvI	GCAGC	3 cut(s)	312, 971, 1435
BccI	CCATC	6 cut(s)	231, 316, 649, 809, 1052, 1301
BceAI	ACGGC	2 cut(s)	559, 1048
BcgI	CGANNNNNNTGC	2 cut(s)	289, 323
BciT130I	CCWGG	3 cut(s)	395, 728, 899
BciVI	GTATCC	1 cut(s)	439
BclI	TGATCA	1 cut(s)	904
BcnI	CCSGG	1 cut(s)	95
BcoDI	GTCTC	2 cut(s)	704, 931
BfaI	CTAG	3 cut(s)	963, 1373, 1492
BfmI	CTRYAG	1 cut(s)	475
BfuI	GTATCC	1 cut(s)	439
BisI	GCNGC	5 cut(s)	85, 301, 312, 960, 1424
BlnI	CCTAGG	1 cut(s)	1372
BlsI	GCNGC	5 cut(s)	86, 302, 313, 961, 1425
Bme1390I	CCNGG	4 cut(s)	95, 395, 728, 899
Bme18I	GGWCC	1 cut(s)	1397
BmgBI	CACGTC	1 cut(s)	465
BmgT120I	GGNCC	1 cut(s)	1397
BmiI	GGNNCC	3 cut(s)	343, 1117, 1399
BmrFI	CCNGG	4 cut(s)	95, 395, 728, 899
BmrI	ACTGGG	1 cut(s)	1475
BmsI	GCATC	1 cut(s)	265
BmuI	ACTGGG	1 cut(s)	1475
Bpu14I	TTCGAA	1 cut(s)	584
BpuEI	CTTGAG	2 cut(s)	98, 1367
BpuMI	CCSGG	1 cut(s)	95
BsaJI	CCNNGG	8 cut(s)	82, 345, 511, 726, 858, 1011, 1257, 1372
BsaWI	WCCGGW	1 cut(s)	495
Bsc4I	CCNNNNNNNGG	4 cut(s)	157, 310, 787, 1438
Bse118I	RCCGGY	4 cut(s)	418, 449, 495, 1159
Bse1I	ACTGG	3 cut(s)	756, 1328, 1470
Bse21I	CCTNAGG	1 cut(s)	191
Bse3DI	GCAATG	1 cut(s)	357
BseBI	CCWGG	3 cut(s)	395, 728, 899
BseDI	CCNNGG	8 cut(s)	82, 345, 511, 726, 858, 1011, 1257, 1372
BseGI	GGATG	2 cut(s)	166, 327
BseLI	CCNNNNNNNGG	4 cut(s)	157, 310, 787, 1438
BseMI	GCAATG	1 cut(s)	357
BseMII	CTCAG	2 cut(s)	205, 1068
BseNI	ACTGG	3 cut(s)	756, 1328, 1470
BseRI	GAGGAG	3 cut(s)	32, 179, 1471
BseXI	GCAGC	3 cut(s)	312, 971, 1435
BsgI	GTGCAG	2 cut(s)	1064, 1471
Bsh1236I	CGCG	4 cut(s)	84, 89, 311, 443
BshFI	GGCC	2 cut(s)	150, 951
BshTI	ACCGGT	1 cut(s)	495
BsiHKAI	GWGCWC	2 cut(s)	189, 635
BsiSI	CCGG	6 cut(s)	95, 304, 419, 450, 496, 1160
BslFI	GGGAC	2 cut(s)	1109, 1383
BslI	CCNNNNNNNGG	4 cut(s)	157, 310, 787, 1438
BsmAI	GTCTC	2 cut(s)	704, 931
BsmFI	GGGAC	2 cut(s)	1109, 1383
BsnI	GGCC	2 cut(s)	150, 951
Bsp119I	TTCGAA	1 cut(s)	584
Bsp1286I	GDGCHC	5 cut(s)	95, 189, 635, 650, 685
Bsp143I	GATC	2 cut(s)	306, 904
Bsp19I	CCATGG	1 cut(s)	345
BspACI	CCGC	7 cut(s)	82, 84, 205, 220, 311, 319, 443
BspANI	GGCC	2 cut(s)	150, 951
BspCNI	CTCAG	2 cut(s)	204, 1067
BspFNI	CGCG	4 cut(s)	84, 89, 311, 443
BspHI	TCATGA	1 cut(s)	1176
BspLI	GGNNCC	3 cut(s)	343, 1117, 1399
BspMAI	CTGCAG	1 cut(s)	479
BspPI	GGATC	1 cut(s)	314
BspQI	GCTCTTC	1 cut(s)	1328
BspT104I	TTCGAA	1 cut(s)	584
BsrDI	GCAATG	1 cut(s)	357
BsrFI	RCCGGY	4 cut(s)	418, 449, 495, 1159
BsrI	ACTGG	3 cut(s)	756, 1328, 1470
BssAI	RCCGGY	4 cut(s)	418, 449, 495, 1159
BssECI	CCNNGG	8 cut(s)	82, 345, 511, 726, 858, 1011, 1257, 1372
BssMI	GATC	2 cut(s)	306, 904
BssSI	CACGAG	2 cut(s)	739, 1086
BssT1I	CCWWGG	5 cut(s)	345, 511, 858, 1257, 1372
Bst2BI	CACGAG	2 cut(s)	739, 1086
Bst2UI	CCWGG	3 cut(s)	395, 728, 899
Bst4CI	ACNGT	5 cut(s)	380, 485, 571, 630, 715
Bst6I	CTCTTC	3 cut(s)	32, 276, 1328
BstBI	TTCGAA	1 cut(s)	584
BstC8I	GCNNGC	4 cut(s)	91, 152, 420, 1161
BstDEI	CTNAG	3 cut(s)	191, 1054, 1450
BstDSI	CCRYGG	2 cut(s)	82, 345
BstF5I	GGATG	2 cut(s)	166, 327
BstFNI	CGCG	4 cut(s)	84, 89, 311, 443
BstHHI	GCGC	1 cut(s)	89
BstKTI	GATC	2 cut(s)	309, 907
BstMAI	GTCTC	2 cut(s)	704, 931
BstMBI	GATC	2 cut(s)	306, 904
BstMWI	GCNNNNNNNGC	5 cut(s)	90, 728, 798, 1169, 1310
BstNI	CCWGG	3 cut(s)	395, 728, 899
BstNSI	RCATGY	1 cut(s)	926
BstSCI	CCNGG	4 cut(s)	93, 393, 726, 897
BstSFI	CTRYAG	1 cut(s)	475
BstUI	CGCG	4 cut(s)	84, 89, 311, 443
BstV1I	GCAGC	3 cut(s)	312, 971, 1435
Bsu36I	CCTNAGG	1 cut(s)	191
BsuI	GTATCC	1 cut(s)	439
BsuRI	GGCC	2 cut(s)	150, 951
BtgI	CCRYGG	2 cut(s)	82, 345
BtrI	CACGTC	1 cut(s)	465
BtsCI	GGATG	2 cut(s)	166, 327
BtsI	GCAGTG	2 cut(s)	460, 799
BtsIMutI	CAGTG	5 cut(s)	460, 635, 799, 1335, 1463
Cac8I	GCNNGC	4 cut(s)	91, 152, 420, 1161
CaiI	CAGNNNCTG	1 cut(s)	394
CciI	TCATGA	1 cut(s)	1176
CfoI	GCGC	1 cut(s)	89
Cfr10I	RCCGGY	4 cut(s)	418, 449, 495, 1159
Cfr13I	GGNCC	1 cut(s)	1397
Cfr42I	CCGCGG	1 cut(s)	85
CsiI	ACCWGGT	1 cut(s)	393
Csp6I	GTAC	4 cut(s)	130, 238, 391, 670
CspAI	ACCGGT	1 cut(s)	495
CspCI	CAANNNNNGTGG	2 cut(s)	775, 810
CviAII	CATG	8 cut(s)	74, 155, 346, 542, 686, 809, 923, 1177
CviQI	GTAC	4 cut(s)	130, 238, 391, 670
DdeI	CTNAG	3 cut(s)	191, 1054, 1450
DpnI	GATC	2 cut(s)	308, 906
DpnII	GATC	2 cut(s)	306, 904
DraI	TTTAAA	1 cut(s)	565
DraIII	CACNNNGTG	1 cut(s)	1088
EaeI	YGGCCR	1 cut(s)	949
Eam1104I	CTCTTC	3 cut(s)	32, 276, 1328
EarI	CTCTTC	3 cut(s)	32, 276, 1328
EciI	GGCGGA	2 cut(s)	209, 334
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	5 cut(s)	345, 511, 858, 1257, 1372
Eco24I	GRGCYC	4 cut(s)	95, 189, 650, 685
Eco32I	GATATC	1 cut(s)	234
Eco47I	GGWCC	1 cut(s)	1397
Eco53kI	GAGCTC	1 cut(s)	187
Eco57I	CTGAAG	2 cut(s)	300, 1234
Eco81I	CCTNAGG	1 cut(s)	191
EcoICRI	GAGCTC	1 cut(s)	187
EcoRII	CCWGG	3 cut(s)	393, 726, 897
EcoRV	GATATC	1 cut(s)	234
EcoT14I	CCWWGG	5 cut(s)	345, 511, 858, 1257, 1372
EcoT22I	ATGCAT	1 cut(s)	691
EcoT38I	GRGCYC	4 cut(s)	95, 189, 650, 685
ErhI	CCWWGG	5 cut(s)	345, 511, 858, 1257, 1372
FaeI	CATG	8 cut(s)	77, 158, 349, 545, 689, 812, 926, 1180
FalI	AAGNNNNNCTT	2 cut(s)	252, 284
FaqI	GGGAC	2 cut(s)	1109, 1383
FatI	CATG	8 cut(s)	73, 154, 345, 541, 685, 808, 922, 1176
FbaI	TGATCA	1 cut(s)	904
Fnu4HI	GCNGC	5 cut(s)	85, 301, 312, 960, 1424
FokI	GGATG	2 cut(s)	173, 334
FriOI	GRGCYC	4 cut(s)	95, 189, 650, 685
Fsp4HI	GCNGC	5 cut(s)	85, 301, 312, 960, 1424
FspBI	CTAG	3 cut(s)	963, 1373, 1492
GlaI	GCGC	1 cut(s)	88
GluI	GCNGC	5 cut(s)	85, 301, 312, 960, 1424
HaeIII	GGCC	2 cut(s)	150, 951
HapII	CCGG	6 cut(s)	95, 304, 419, 450, 496, 1160
HhaI	GCGC	1 cut(s)	89
Hin1II	CATG	8 cut(s)	77, 158, 349, 545, 689, 812, 926, 1180
Hin6I	GCGC	1 cut(s)	87
HinP1I	GCGC	1 cut(s)	87
HinfI	GANTC	4 cut(s)	1007, 1050, 1127, 1263
HpaII	CCGG	6 cut(s)	95, 304, 419, 450, 496, 1160
HphI	GGTGA	3 cut(s)	824, 1016, 1212
Hpy166II	GTNNAC	1 cut(s)	609
Hpy188I	TCNGA	5 cut(s)	373, 413, 1012, 1057, 1152
Hpy188III	TCNNGA	3 cut(s)	272, 1177, 1346
Hpy8I	GTNNAC	1 cut(s)	609
HpyAV	CCTTC	3 cut(s)	237, 278, 510
HpyCH4III	ACNGT	5 cut(s)	380, 485, 571, 630, 715
HpyCH4IV	ACGT	2 cut(s)	464, 759
HpyF10VI	GCNNNNNNNGC	5 cut(s)	90, 728, 798, 1169, 1310
HpyF3I	CTNAG	3 cut(s)	191, 1054, 1450
HpySE526I	ACGT	2 cut(s)	464, 759
Hsp92II	CATG	8 cut(s)	77, 158, 349, 545, 689, 812, 926, 1180
HspAI	GCGC	1 cut(s)	87
KroI	GCCGGC	2 cut(s)	418, 1159
KroNI	GCCGGC	2 cut(s)	420, 1161
Ksp22I	TGATCA	1 cut(s)	904
KspI	CCGCGG	1 cut(s)	85
Kzo9I	GATC	2 cut(s)	306, 904
LguI	GCTCTTC	1 cut(s)	1328
LmnI	GCTCC	8 cut(s)	10, 184, 192, 204, 347, 638, 1310, 1480
Lsp1109I	GCAGC	3 cut(s)	312, 971, 1435
LweI	GCATC	1 cut(s)	265
MabI	ACCWGGT	1 cut(s)	393
MaeI	CTAG	3 cut(s)	963, 1373, 1492
MaeII	ACGT	2 cut(s)	464, 759
MaeIII	GTNAC	3 cut(s)	455, 1004, 1218
MalI	GATC	2 cut(s)	308, 906
MboI	GATC	2 cut(s)	306, 904
MboII	GAAGA	9 cut(s)	16, 19, 272, 293, 546, 549, 573, 1345, 1375
MhlI	GDGCHC	5 cut(s)	95, 189, 635, 650, 685
MlsI	TGGCCA	1 cut(s)	951
MluCI	AATT	8 cut(s)	16, 402, 587, 931, 1214, 1286, 1293, 1442
MluNI	TGGCCA	1 cut(s)	951
MlyI	GAGTC	1 cut(s)	1001
MmeI	TCCRAC	1 cut(s)	649
Mox20I	TGGCCA	1 cut(s)	951
Mph1103I	ATGCAT	1 cut(s)	691
MroNI	GCCGGC	2 cut(s)	418, 1159
MroXI	GAANNNNTTC	2 cut(s)	267, 848
MscI	TGGCCA	1 cut(s)	951
MseI	TTAA	5 cut(s)	293, 399, 405, 564, 1107
MslI	CAYNNNNRTG	5 cut(s)	144, 159, 350, 813, 876
Msp20I	TGGCCA	1 cut(s)	951
MspA1I	CMGCKG	1 cut(s)	84
MspI	CCGG	6 cut(s)	95, 304, 419, 450, 496, 1160
MspR9I	CCNGG	4 cut(s)	95, 395, 728, 899
MvaI	CCWGG	3 cut(s)	395, 728, 899
MvnI	CGCG	4 cut(s)	84, 89, 311, 443
MwoI	GCNNNNNNNGC	5 cut(s)	90, 728, 798, 1169, 1310
NaeI	GCCGGC	2 cut(s)	420, 1161
NciI	CCSGG	1 cut(s)	95
NcoI	CCATGG	1 cut(s)	345
NdeII	GATC	2 cut(s)	306, 904
NgoMIV	GCCGGC	2 cut(s)	418, 1159
NlaIII	CATG	8 cut(s)	77, 158, 349, 545, 689, 812, 926, 1180
NlaIV	GGNNCC	3 cut(s)	343, 1117, 1399
NmuCI	GTSAC	3 cut(s)	455, 1004, 1218
NsiI	ATGCAT	1 cut(s)	691
NspI	RCATGY	1 cut(s)	926
NspV	TTCGAA	1 cut(s)	584
OliI	CACNNNNGTG	1 cut(s)	876
PagI	TCATGA	1 cut(s)	1176
PciI	ACATGT	1 cut(s)	922
PciSI	GCTCTTC	1 cut(s)	1328
PcsI	WCGNNNNNNNCGW	1 cut(s)	1008
PdiI	GCCGGC	2 cut(s)	420, 1161
PdmI	GAANNNNTTC	2 cut(s)	267, 848
PfeI	GAWTC	3 cut(s)	1050, 1127, 1263
PflFI	GACNNNGTC	1 cut(s)	215
PinAI	ACCGGT	1 cut(s)	495
PkrI	GCNGC	5 cut(s)	86, 302, 313, 961, 1425
PleI	GAGTC	1 cut(s)	1001
PpsI	GAGTC	1 cut(s)	1001
PscI	ACATGT	1 cut(s)	922
PsiI	TTATAA	1 cut(s)	144
Psp124BI	GAGCTC	1 cut(s)	189
Psp6I	CCWGG	3 cut(s)	393, 726, 897
PspGI	CCWGG	3 cut(s)	393, 726, 897
PspN4I	GGNNCC	3 cut(s)	343, 1117, 1399
PspPI	GGNCC	1 cut(s)	1397
PstI	CTGCAG	1 cut(s)	479
PstNI	CAGNNNCTG	1 cut(s)	394
PsyI	GACNNNGTC	1 cut(s)	215
RsaI	GTAC	4 cut(s)	131, 239, 392, 671
RsaNI	GTAC	4 cut(s)	130, 238, 391, 670
RseI	CAYNNNNRTG	5 cut(s)	144, 159, 350, 813, 876
SacI	GAGCTC	1 cut(s)	189
SacII	CCGCGG	1 cut(s)	85
SapI	GCTCTTC	1 cut(s)	1328
SaqAI	TTAA	5 cut(s)	293, 399, 405, 564, 1107
SatI	GCNGC	5 cut(s)	85, 301, 312, 960, 1424
Sau3AI	GATC	2 cut(s)	306, 904
Sau96I	GGNCC	1 cut(s)	1397
SchI	GAGTC	1 cut(s)	1001
ScrFI	CCNGG	4 cut(s)	95, 395, 728, 899
SduI	GDGCHC	5 cut(s)	95, 189, 635, 650, 685
SexAI	ACCWGGT	1 cut(s)	393
SfaNI	GCATC	1 cut(s)	265
SfcI	CTRYAG	1 cut(s)	475
Sfr303I	CCGCGG	1 cut(s)	85
SfuI	TTCGAA	1 cut(s)	584
SgrBI	CCGCGG	1 cut(s)	85
SinI	GGWCC	1 cut(s)	1397
SmiMI	CAYNNNNRTG	5 cut(s)	144, 159, 350, 813, 876
SmlI	CTYRAG	2 cut(s)	113, 1346
SmoI	CTYRAG	2 cut(s)	113, 1346
Sse9I	AATT	8 cut(s)	16, 402, 587, 931, 1214, 1286, 1293, 1442
SsiI	CCGC	7 cut(s)	82, 84, 205, 220, 311, 319, 443
SspI	AATATT	1 cut(s)	291
SspMI	CTAG	3 cut(s)	963, 1373, 1492
SstI	GAGCTC	1 cut(s)	189
StyD4I	CCNGG	4 cut(s)	93, 393, 726, 897
StyI	CCWWGG	5 cut(s)	345, 511, 858, 1257, 1372
TaaI	ACNGT	5 cut(s)	380, 485, 571, 630, 715
TaiI	ACGT	2 cut(s)	467, 762
TaqI	TCGA	3 cut(s)	584, 644, 1202
TasI	AATT	8 cut(s)	16, 402, 587, 931, 1214, 1286, 1293, 1442
TauI	GCSGC	2 cut(s)	87, 314
TfiI	GAWTC	3 cut(s)	1050, 1127, 1263
Tru1I	TTAA	5 cut(s)	293, 399, 405, 564, 1107
Tru9I	TTAA	5 cut(s)	293, 399, 405, 564, 1107
TscAI	CASTG	5 cut(s)	460, 635, 799, 1335, 1470
TseFI	GTSAC	3 cut(s)	455, 1004, 1218
TseI	GCWGC	3 cut(s)	300, 959, 1423
Tsp45I	GTSAC	3 cut(s)	455, 1004, 1218
TspDTI	ATGAA	5 cut(s)	17, 19, 62, 995, 1193
TspGWI	ACGGA	1 cut(s)	37
TspRI	CASTG	5 cut(s)	460, 635, 799, 1335, 1470
Tth111I	GACNNNGTC	1 cut(s)	215
VpaK11BI	GGWCC	1 cut(s)	1397
XapI	RAATTY	4 cut(s)	16, 931, 1286, 1442
XceI	RCATGY	1 cut(s)	926
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1089
XmaJI	CCTAGG	1 cut(s)	1372
XmnI	GAANNNNTTC	2 cut(s)	267, 848
XspI	CTAG	3 cut(s)	963, 1373, 1492
Zsp2I	ATGCAT	1 cut(s)	691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.