Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005873

Physical Location & Seq

Genomic Coordinates Reverse (-)

3126237 .. 3127745

Gene Structure 1509 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00220750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGATGAAGGGGAGGATGTCAACTAGTTCAACAACATTCCTCTTCATCATTTTCTTGCTTTCTGCCACTCATCATGGATTTTTGCACGTTTGTGCAAATGGTGATCATACCACCATGCGGTTGCCGCTAATTCACAAGTACTCTCCTCATTTCAATGGAGGAGTTGGTTTGATCAACAAAACCGATATAGATATCATGAAAGAGCTTCACCAACATGACATAATCCGCCACCAAATGATTTTCCAAAGGAATAGAGATGATCATCCAATCCCAAGGCGAGAGGACTCAGAAACTAACTCATCCTTTGCAATGCCGGTGCATTCGGGTTTTGATATGCTGTCGGGGTTGTATTGGGTCGAAGTAAAAATTGGAACACCTCCCAAGACCTTCTTGTTGGCTGCAGATACAGGGAGTGACTTGACATGGGTCAAATGCAAATACGATAGAACGTTGACAAAGGAGGCCAAACAAAAAATGATAGACGATCGCAAGAAGAAGAAACACCATCATGGCGATCTCAAAAAGTCATTAATTTTTCTCAATAAAGGTAGGGAATTTCGTGCTGATCTATCATCGACATTCAAACCTGTTCCATGCTCCGATGGTATGTGCAGACAAGGGCTTGCACACATGATGTCTTATAAAGACTGCCCAACCCCAACGTCGCTTTGCAGATACACTTACAAGTACATGGGAGGGGAGGGAGCAAATGGTTTCTTTGGGTATGACACTATAACAGTACCCCTCGCAAATGGTAGCAAAACAAAGCTGCCTAATGTGACAATCGGCTGCACAGATTCCAACCAAAAGTTAAAGCAAGGTGATGGCATTCTAGGTTTAGGCTTCGGTGACTACTCATTCTCCGAGACAGTAGTTTCTTATAAGTTTGCTGGCAAGTTCTCTTACTGCCTAATGTTTCATAGGAGCAACTCGAGTGTGTTGAGTTATCTTACATTTGGTCCCAATAAACAAACTGTCAACAGCCCTATGAGGTACACAAAGTTGATTATGAGGAAGCAGAACGGAAGTAACTCTAATTTATATGGCGTAAACATAACGGGCATCTCAATCGGGGGCACATTATTGAAGATACCAAGTCAATTTTGGGATGCAACATCGCGTAATGGTGGTGTAGTGCTTGATTCAGGCTCAACCAACACATTTCTATCCCTACCAGCTTACAAGGCTGTCATGGACGAATTGACAGATGCTTTGTCAAAATATAATAGTTTCGTGCTCAAAGAGCTCCCATTTGAATTCTGCTTCAATGATAAAGGATATGACGAGTCTTCGGTGCCAAGACTTGCATTTCATTTTGCAGATGGTGTTCGATATGAGCCACCGGTGAGAAACTATGTTGTACCTGTGACAACGGGGATCAAGTGTATTGGCTTTGTTCCATCTCCCGATGGCAAGTCTGTGATTGGCAACCAAATGCAGCAAAACCATATTTGGGAATTTGATATTTCACGTGGTACTGTGGGTTTTGCTCCTTCCGGCTGCACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009743 GO:0009744 GO:0009888 GO:0009987 GO:0010019 GO:0010033 GO:0010073 GO:0010074 GO:0010492 GO:0016787 GO:0019538 GO:0019827 GO:0023052 GO:0030163 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048507 GO:0048511 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070001 GO:0070011 GO:0071704 GO:0098727 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

502

Amino Acids

56.07

Weight (kDa)

9.13

Isoelectric Point (pI)

31.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAXi_N	PF14543	117 - 320	1.3e-37	Xylanase inhibitor N-terminal
Asp	PF00026	117 - 498	4e-17	Eukaryotic aspartyl protease
TAXi_C	PF14541	348 - 497	4.8e-32	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12700 AT3G12700 AT3G12700
fragaria_vesca	FvH4_3g13820 FvH4_5g37920
malus_domestica	MD05G1240800.v1.1 MD10G1219200.v1.1
prunus_persica	Prupe.4G124800_v2.0.a1
pyrus_communis	pycom05g21700 pycom10g18470
rosa_chinensis	RchiOBHm_Chr5g0023001 RchiOBHm_Chr7g0239861
rosa_laevigata	RLG00000000724 RLG00000032688
rosa_roxburghii	Rroxscaffold_1G00056340 Rroxscaffold_3G00220750
rosa_rugosa	Rorug05G0076400 Rorug07G0330600
rosa_samantha	Rh5AG162600 Rh5BG161800 Rh5CG176500 Rh5DG163200 Rh7AG484100 Rh7BG457200 Rh7CG501600 Rh7DG469800
rosa_wichuraiana	Rw5G014650 Rw7G040610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	642, 882
AccB1I	GGYRCC	1 cut(s)	1294
AccII	CGCG	1 cut(s)	1120
AciI	CCGC	3 cut(s)	118, 125, 226
AclI	AACGTT	1 cut(s)	449
AclWI	GGATC	1 cut(s)	1385
AcsI	RAATTY	3 cut(s)	554, 1256, 1457
AcvI	CACGTG	1 cut(s)	1472
AfaI	GTAC	6 cut(s)	140, 689, 741, 995, 1362, 1477
AfiI	CCNNNNNNNGG	2 cut(s)	117, 1453
AgeI	ACCGGT	1 cut(s)	1342
AgsI	TTSAA	6 cut(s)	30, 154, 583, 1087, 1256, 1267
AhlI	ACTAGT	1 cut(s)	23
AjuI	GAANNNNNNNTTGG	2 cut(s)	351, 383
AleI	CACNNNNGTG	1 cut(s)	90
AluBI	AGCT	4 cut(s)	205, 769, 1178, 1246
AluI	AGCT	4 cut(s)	205, 769, 1178, 1246
Alw21I	GWGCWC	2 cut(s)	1239, 1248
Alw26I	GTCTC	1 cut(s)	860
AlwI	GGATC	1 cut(s)	1385
Ama87I	CYCGRG	1 cut(s)	931
AoxI	GGCC	1 cut(s)	462
ApeKI	GCWGC	5 cut(s)	398, 769, 789, 1438, 1500
ApoI	RAATTY	3 cut(s)	554, 1256, 1457
ArsI	GACNNNNNNTTYG	2 cut(s)	1195, 1227
AseI	ATTAAT	1 cut(s)	530
AsiGI	ACCGGT	1 cut(s)	1342
AspS9I	GGNCC	1 cut(s)	959
AsuHPI	GGTGA	5 cut(s)	113, 200, 833, 860, 1357
AvaI	CYCGRG	1 cut(s)	931
AvaII	GGWCC	1 cut(s)	959
BaeGI	GKGCMC	1 cut(s)	1079
BanI	GGYRCC	1 cut(s)	1294
BanII	GRGCYC	1 cut(s)	1248
BbrPI	CACGTG	1 cut(s)	1472
BbsI	GAAGAC	1 cut(s)	1281
Bbv12I	GWGCWC	2 cut(s)	1239, 1248
BbvI	GCAGC	5 cut(s)	385, 756, 776, 1450, 1487
BccI	CCATC	6 cut(s)	513, 596, 818, 1316, 1403, 1408
BcgI	CGANNNNNNTGC	2 cut(s)	1051, 1085
BclI	TGATCA	3 cut(s)	103, 171, 259
BcoDI	GTCTC	1 cut(s)	860
BcuI	ACTAGT	1 cut(s)	23
BfaI	CTAG	3 cut(s)	24, 833, 1507
BfmI	CTRYAG	1 cut(s)	399
BisI	GCNGC	6 cut(s)	125, 399, 770, 790, 1439, 1501
BlsI	GCNGC	6 cut(s)	126, 400, 771, 791, 1440, 1502
BmcAI	AGTACT	1 cut(s)	140
Bme18I	GGWCC	1 cut(s)	959
BmeT110I	CYCGRG	1 cut(s)	931
BmgT120I	GGNCC	1 cut(s)	959
BmiI	GGNNCC	2 cut(s)	961, 1296
BmsI	GCATC	3 cut(s)	1071, 1099, 1198
BoxI	GACNNNNGTC	1 cut(s)	425
BpiI	GAAGAC	1 cut(s)	1281
BsaAI	YACGTR	1 cut(s)	1472
BsaBI	GATNNNNATC	1 cut(s)	261
BsaJI	CCNNGG	1 cut(s)	272
BsaWI	WCCGGW	1 cut(s)	1342
BsaXI	ACNNNNNCTCC	2 cut(s)	845, 875
Bsc4I	CCNNNNNNNGG	2 cut(s)	117, 1453
Bse118I	RCCGGY	2 cut(s)	313, 1342
Bse3DI	GCAATG	1 cut(s)	315
Bse8I	GATNNNNATC	1 cut(s)	261
BseDI	CCNNGG	1 cut(s)	272
BseGI	GGATG	4 cut(s)	21, 262, 299, 1114
BseJI	GATNNNNATC	1 cut(s)	261
BseLI	CCNNNNNNNGG	2 cut(s)	117, 1453
BseMI	GCAATG	1 cut(s)	315
BseMII	CTCAG	1 cut(s)	300
BseRI	GAGGAG	2 cut(s)	135, 174
BseSI	GKGCMC	1 cut(s)	1079
BseXI	GCAGC	5 cut(s)	385, 756, 776, 1450, 1487
BsgI	GTGCAG	3 cut(s)	631, 775, 1486
Bsh1236I	CGCG	1 cut(s)	1120
Bsh1285I	CGRYCG	1 cut(s)	487
BshFI	GGCC	1 cut(s)	464
BshNI	GGYRCC	1 cut(s)	1294
BshTI	ACCGGT	1 cut(s)	1342
BsiEI	CGRYCG	1 cut(s)	487
BsiHKAI	GWGCWC	2 cut(s)	1239, 1248
BsiHKCI	CYCGRG	1 cut(s)	931
BsiSI	CCGG	3 cut(s)	314, 1343, 1497
BslFI	GGGAC	1 cut(s)	945
BslI	CCNNNNNNNGG	2 cut(s)	117, 1453
BsmAI	GTCTC	1 cut(s)	860
BsmFI	GGGAC	1 cut(s)	945
BsmI	GAATGC	2 cut(s)	319, 828
BsnI	GGCC	1 cut(s)	464
BsoBI	CYCGRG	1 cut(s)	931
Bsp1286I	GDGCHC	3 cut(s)	1079, 1239, 1248
Bsp143I	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
BspACI	CCGC	3 cut(s)	118, 125, 226
BspANI	GGCC	1 cut(s)	464
BspCNI	CTCAG	1 cut(s)	299
BspFNI	CGCG	1 cut(s)	1120
BspHI	TCATGA	1 cut(s)	195
BspLI	GGNNCC	2 cut(s)	961, 1296
BspMAI	CTGCAG	1 cut(s)	403
BspPI	GGATC	1 cut(s)	1385
BspT107I	GGYRCC	1 cut(s)	1294
BsrDI	GCAATG	1 cut(s)	315
BsrFI	RCCGGY	2 cut(s)	313, 1342
BssAI	RCCGGY	2 cut(s)	313, 1342
BssECI	CCNNGG	1 cut(s)	272
BssMI	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
BssT1I	CCWWGG	1 cut(s)	272
Bst4CI	ACNGT	4 cut(s)	739, 871, 976, 1480
Bst6I	CTCTTC	1 cut(s)	47
BstBAI	YACGTR	1 cut(s)	1472
BstC8I	GCNNGC	2 cut(s)	624, 892
BstDEI	CTNAG	1 cut(s)	286
BstF5I	GGATG	4 cut(s)	21, 262, 299, 1114
BstFNI	CGCG	1 cut(s)	1120
BstKTI	GATC	7 cut(s)	106, 174, 262, 487, 517, 568, 1380
BstMAI	GTCTC	1 cut(s)	860
BstMBI	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
BstMCI	CGRYCG	1 cut(s)	487
BstMWI	GCNNNNNNNGC	3 cut(s)	124, 1184, 1243
BstPAI	GACNNNNGTC	1 cut(s)	425
BstSFI	CTRYAG	1 cut(s)	399
BstSLI	GKGCMC	1 cut(s)	1079
BstUI	CGCG	1 cut(s)	1120
BstV1I	GCAGC	5 cut(s)	385, 756, 776, 1450, 1487
BstV2I	GAAGAC	1 cut(s)	1281
BsuRI	GGCC	1 cut(s)	464
BtgZI	GCGATG	1 cut(s)	1101
BtsCI	GGATG	4 cut(s)	21, 262, 299, 1114
Cac8I	GCNNGC	2 cut(s)	624, 892
CciI	TCATGA	1 cut(s)	195
Cfr10I	RCCGGY	2 cut(s)	313, 1342
Cfr13I	GGNCC	1 cut(s)	959
Csp6I	GTAC	6 cut(s)	139, 688, 740, 994, 1361, 1476
CspAI	ACCGGT	1 cut(s)	1342
CviQI	GTAC	6 cut(s)	139, 688, 740, 994, 1361, 1476
DdeI	CTNAG	1 cut(s)	286
DpnI	GATC	7 cut(s)	105, 173, 261, 486, 516, 567, 1379
DpnII	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
Eam1104I	CTCTTC	1 cut(s)	47
EarI	CTCTTC	1 cut(s)	47
EciI	GGCGGA	1 cut(s)	215
Ecl136II	GAGCTC	1 cut(s)	1246
Eco130I	CCWWGG	1 cut(s)	272
Eco24I	GRGCYC	1 cut(s)	1248
Eco32I	GATATC	1 cut(s)	193
Eco47I	GGWCC	1 cut(s)	959
Eco53kI	GAGCTC	1 cut(s)	1246
Eco72I	CACGTG	1 cut(s)	1472
Eco88I	CYCGRG	1 cut(s)	931
EcoICRI	GAGCTC	1 cut(s)	1246
EcoRI	GAATTC	1 cut(s)	1256
EcoRV	GATATC	1 cut(s)	193
EcoT14I	CCWWGG	1 cut(s)	272
EcoT38I	GRGCYC	1 cut(s)	1248
ErhI	CCWWGG	1 cut(s)	272
FalI	AAGNNNNNCTT	2 cut(s)	374, 406
FaqI	GGGAC	1 cut(s)	945
FbaI	TGATCA	3 cut(s)	103, 171, 259
Fnu4HI	GCNGC	6 cut(s)	125, 399, 770, 790, 1439, 1501
FokI	GGATG	4 cut(s)	28, 249, 286, 1121
FriOI	GRGCYC	1 cut(s)	1248
Fsp4HI	GCNGC	6 cut(s)	125, 399, 770, 790, 1439, 1501
FspBI	CTAG	3 cut(s)	24, 833, 1507
GluI	GCNGC	6 cut(s)	125, 399, 770, 790, 1439, 1501
HaeIII	GGCC	1 cut(s)	464
HapII	CCGG	3 cut(s)	314, 1343, 1497
HincII	GTYRAC	3 cut(s)	21, 453, 979
HindII	GTYRAC	3 cut(s)	21, 453, 979
HinfI	GANTC	4 cut(s)	284, 797, 1142, 1286
HpaII	CCGG	3 cut(s)	314, 1343, 1497
HphI	GGTGA	5 cut(s)	113, 200, 833, 860, 1357
Hpy166II	GTNNAC	5 cut(s)	21, 453, 979, 996, 1051
Hpy188I	TCNGA	3 cut(s)	289, 601, 865
Hpy188III	TCNNGA	2 cut(s)	196, 1406
Hpy8I	GTNNAC	5 cut(s)	21, 453, 979, 996, 1051
Hpy99I	CGWCG	1 cut(s)	667
HpyAV	CCTTC	2 cut(s)	397, 1503
HpyCH4III	ACNGT	4 cut(s)	739, 871, 976, 1480
HpyCH4IV	ACGT	4 cut(s)	87, 449, 662, 1471
HpyF10VI	GCNNNNNNNGC	3 cut(s)	124, 1184, 1243
HpyF3I	CTNAG	1 cut(s)	286
HpySE526I	ACGT	4 cut(s)	87, 449, 662, 1471
Ksp22I	TGATCA	3 cut(s)	103, 171, 259
Kzo9I	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
LmnI	GCTCC	5 cut(s)	602, 704, 924, 1251, 1495
LpnPI	CCDG	8 cut(s)	327, 393, 600, 876, 1131, 1188, 1356, 1377
Lsp1109I	GCAGC	5 cut(s)	385, 756, 776, 1450, 1487
LweI	GCATC	3 cut(s)	1071, 1099, 1198
MaeI	CTAG	3 cut(s)	24, 833, 1507
MaeII	ACGT	4 cut(s)	87, 449, 662, 1471
MaeIII	GTNAC	5 cut(s)	413, 778, 848, 1028, 1366
MalI	GATC	7 cut(s)	105, 173, 261, 486, 516, 567, 1379
MboI	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
MboII	GAAGA	5 cut(s)	34, 505, 508, 1099, 1281
MhlI	GDGCHC	3 cut(s)	1079, 1239, 1248
MluCI	AATT	9 cut(s)	129, 366, 531, 554, 1036, 1100, 1199, 1256, 1457
MlyI	GAGTC	2 cut(s)	278, 1295
MmeI	TCCRAC	1 cut(s)	825
MseI	TTAA	2 cut(s)	530, 812
MslI	CAYNNNNRTG	4 cut(s)	90, 153, 213, 507
MspI	CCGG	3 cut(s)	314, 1343, 1497
Mva1269I	GAATGC	2 cut(s)	319, 828
MvnI	CGCG	1 cut(s)	1120
MwoI	GCNNNNNNNGC	3 cut(s)	124, 1184, 1243
NdeII	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
NlaIV	GGNNCC	2 cut(s)	961, 1296
NmuCI	GTSAC	4 cut(s)	413, 778, 848, 1366
OliI	CACNNNNGTG	1 cut(s)	90
PaeR7I	CTCGAG	1 cut(s)	931
PagI	TCATGA	1 cut(s)	195
PctI	GAATGC	2 cut(s)	319, 828
PfeI	GAWTC	2 cut(s)	797, 1142
PinAI	ACCGGT	1 cut(s)	1342
PkrI	GCNGC	6 cut(s)	126, 400, 771, 791, 1440, 1502
Ple19I	CGATCG	1 cut(s)	487
PleI	GAGTC	2 cut(s)	278, 1294
PmaCI	CACGTG	1 cut(s)	1472
PmlI	CACGTG	1 cut(s)	1472
PpsI	GAGTC	2 cut(s)	278, 1294
Ppu21I	YACGTR	1 cut(s)	1472
PshAI	GACNNNNGTC	1 cut(s)	425
PshBI	ATTAAT	1 cut(s)	530
PsiI	TTATAA	2 cut(s)	642, 882
Psp124BI	GAGCTC	1 cut(s)	1248
Psp1406I	AACGTT	1 cut(s)	449
PspCI	CACGTG	1 cut(s)	1472
PspN4I	GGNNCC	2 cut(s)	961, 1296
PspPI	GGNCC	1 cut(s)	959
PspXI	VCTCGAGB	1 cut(s)	931
PstI	CTGCAG	1 cut(s)	403
PvuI	CGATCG	1 cut(s)	487
RsaI	GTAC	6 cut(s)	140, 689, 741, 995, 1362, 1477
RsaNI	GTAC	6 cut(s)	139, 688, 740, 994, 1361, 1476
RseI	CAYNNNNRTG	4 cut(s)	90, 153, 213, 507
SacI	GAGCTC	1 cut(s)	1248
SaqAI	TTAA	2 cut(s)	530, 812
SatI	GCNGC	6 cut(s)	125, 399, 770, 790, 1439, 1501
Sau3AI	GATC	7 cut(s)	103, 171, 259, 484, 514, 565, 1377
Sau96I	GGNCC	1 cut(s)	959
ScaI	AGTACT	1 cut(s)	140
SchI	GAGTC	2 cut(s)	278, 1295
SduI	GDGCHC	3 cut(s)	1079, 1239, 1248
SfaNI	GCATC	3 cut(s)	1071, 1099, 1198
SfcI	CTRYAG	1 cut(s)	399
Sfr274I	CTCGAG	1 cut(s)	931
SgrAI	CRCCGGYG	1 cut(s)	1342
SinI	GGWCC	1 cut(s)	959
SlaI	CTCGAG	1 cut(s)	931
SmiMI	CAYNNNNRTG	4 cut(s)	90, 153, 213, 507
SmlI	CTYRAG	1 cut(s)	931
SmoI	CTYRAG	1 cut(s)	931
SpeI	ACTAGT	1 cut(s)	23
Sse9I	AATT	9 cut(s)	129, 366, 531, 554, 1036, 1100, 1199, 1256, 1457
SsiI	CCGC	3 cut(s)	118, 125, 226
SspMI	CTAG	3 cut(s)	24, 833, 1507
SstI	GAGCTC	1 cut(s)	1248
StyI	CCWWGG	1 cut(s)	272
TaaI	ACNGT	4 cut(s)	739, 871, 976, 1480
TaiI	ACGT	4 cut(s)	90, 452, 665, 1474
TaqI	TCGA	4 cut(s)	357, 575, 932, 1330
TasI	AATT	9 cut(s)	129, 366, 531, 554, 1036, 1100, 1199, 1256, 1457
TatI	WGTACW	2 cut(s)	138, 687
TauI	GCSGC	1 cut(s)	127
TfiI	GAWTC	2 cut(s)	797, 1142
Tru1I	TTAA	2 cut(s)	530, 812
Tru9I	TTAA	2 cut(s)	530, 812
TseFI	GTSAC	4 cut(s)	413, 778, 848, 1366
TseI	GCWGC	5 cut(s)	398, 769, 789, 1438, 1500
Tsp45I	GTSAC	4 cut(s)	413, 778, 848, 1366
TspDTI	ATGAA	5 cut(s)	20, 34, 212, 908, 1301
TspGWI	ACGGA	1 cut(s)	1038
VpaK11BI	GGWCC	1 cut(s)	959
VspI	ATTAAT	1 cut(s)	530
XapI	RAATTY	3 cut(s)	554, 1256, 1457
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1101
XhoI	CTCGAG	1 cut(s)	931
XspI	CTAG	3 cut(s)	24, 833, 1507
ZrmI	AGTACT	1 cut(s)	140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.