Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0005873

Physical Location & Seq

Genomic Coordinates Forward (+)

16841342 .. 16844318

Gene Structure 2977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG161800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGAAGGGGAGGCCAAACGCATTCCTCTTCATCATCTTCTTGCTCTCTGCCCTTCACGGGATTTTCTCTGTTCATTCATTTCTCAATGAATTGAAACAAGACGAACCCATGAGATTAGAGCTCATTCACAGGCACTCTCCGAAGCTCAATGGGCATGGTTTGCGTGACGAAATGCCGAAAACGCAGCTAGAACTCATTGAAGAGCTCCACCGCCACGACCTCATTCGCCAACAAATGATCTCCCGCAAGAGGCAACACCACCATCATCACATTCAAACTGGCCTAAGGAGAAATGCATTGGAAACAGCTTCCATTGCAATGCCATTGAGTTCGGGTTGGGATTTCGGTGTCGGGCAGTACCTTGTTCAGGTTAAAGTGGGAACACCTTCACAGAGATTCCTGTTGATTGCAGACACAGGGAGTGACTTGACATGGATGAAGTGCAAGTACCGGTGCGGTGAGAAATGTGGGTTGAAAAGAGCAAAGATGAAGAAGAACAAGAAGAAGGTGTTTCGTGCCACTCAATCTTCAACCTTCAAAACTATCACTTGCTCTTCTGAGATGTGCAAGTTCGAGCTTGACTTCTCTCTCACAATATGTCCAACCCCATTGAGCCGTTGCAAATATGATTATAGGTACGCAGAAAAATCTGGTGCATTGGGCTTCTTTGCGAATGAGACTGTAAGAGTACCCCTTACGAATGGTAGGAGCGCGAAGCTGAATGATGTGCTAATTGGCTGCACAGAGTCCATCGAGGGCCCTAAAGAGGCAAGCATCTGGGGAGGTGATGGGATATTGGGTCTAGGGTTCAGCAAGCACTCATTCGTTGCGAAAGCTGCTTCAAACTTGGGTGACAAGTTTTCCTATTGCCTAATTGATCACATGAGCAACAAGAATGTGTCAAGCTATCTCACATTTGGTGGAAGTGCTGAACCCGCACTGCGAAACAGCATGAGTTACACAAAGCTTGCTTTGGGTGGCCCTAAAATTGGCCCATTTTACGCTGTAAACTTGGTGGGGATATCAGTCAGGGGCAAAATGCTAAAGATACCAAATGAAGTGTGGGTTGAAAACCTTGGTGGTGGAACAATTGTTGATTCAGGCACAAGCCTCACGTTTCTAACCTCACCAGCTTACAAACCTGTCATGGCTGAATTGACAATGGCTTTGTCCAAGTACGAAAAAATACCGTTGGATGCATTTGAGTTCTGCTTCAATTCCACTGGTTATGATGAGTCTTCAGTGCCGAGATTTGCAGTTCATTTTGCAGATGGAGCTAAATTTGAGCCCCCAGTGAAGAGCTATGTTATTGATGTTGCTGTACAGACAAAGTGTCTCGGGTTTATGTCAGCTATATTTCCTGGCACCATTGTTATTGGCAACATAATGCAACAAAATTATTTGTGGGAATTCGATTTGCGGGGAGGTACATTGGGTTTTGCTCCTTCAAGCTGCACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006508 GO:0006807 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009507 GO:0009536 GO:0009743 GO:0009744 GO:0009888 GO:0009987 GO:0010019 GO:0010033 GO:0010073 GO:0010074 GO:0010492 GO:0016787 GO:0019538 GO:0019827 GO:0023052 GO:0030163 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048507 GO:0048511 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070001 GO:0070011 GO:0071704 GO:0098727 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

486

Amino Acids

53.96

Weight (kDa)

9.19

Isoelectric Point (pI)

42.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	119 - 482	8.4e-21	Eukaryotic aspartyl protease
TAXi_N	PF14543	120 - 308	5.8e-44	Xylanase inhibitor N-terminal
TAXi_C	PF14541	334 - 481	1.9e-33	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12700 AT3G12700 AT3G12700
fragaria_vesca	FvH4_3g13820 FvH4_5g37920
malus_domestica	MD05G1240800.v1.1 MD10G1219200.v1.1
prunus_persica	Prupe.4G124800_v2.0.a1
pyrus_communis	pycom05g21700 pycom10g18470
rosa_chinensis	RchiOBHm_Chr5g0023001 RchiOBHm_Chr7g0239861
rosa_laevigata	RLG00000000724 RLG00000032688
rosa_roxburghii	Rroxscaffold_1G00056340 Rroxscaffold_3G00220750
rosa_rugosa	Rorug05G0076400 Rorug07G0330600
rosa_samantha	Rh5AG162600 Rh5BG161800 Rh5CG176500 Rh5DG163200 Rh7AG484100 Rh7BG457200 Rh7CG501600 Rh7DG469800
rosa_wichuraiana	Rw5G014650 Rw7G040610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1364
AccII	CGCG	1 cut(s)	713
AciI	CCGC	5 cut(s)	211, 244, 456, 936, 1420
AcsI	RAATTY	2 cut(s)	1280, 1409
AcuI	CTGAAG	1 cut(s)	1224
AfaI	GTAC	7 cut(s)	359, 449, 638, 690, 1178, 1323, 1429
AfiI	CCNNNNNNNGG	5 cut(s)	57, 249, 367, 766, 989
AgeI	ACCGGT	1 cut(s)	450
AhdI	GACNNNNNGTC	1 cut(s)	1332
AjnI	CCWGG	1 cut(s)	1360
Alw21I	GWGCWC	2 cut(s)	123, 207
Alw26I	GTCTC	2 cut(s)	671, 1340
Ama87I	CYCGRG	1 cut(s)	1337
AoxI	GGCC	5 cut(s)	11, 280, 757, 979, 991
ApaI	GGGCCC	1 cut(s)	761
ApeKI	GCWGC	4 cut(s)	184, 738, 836, 1452
ApoI	RAATTY	2 cut(s)	1280, 1409
ArsI	GACNNNNNNTTYG	2 cut(s)	1150, 1182
AsiGI	ACCGGT	1 cut(s)	450
Asp700I	GAANNNNTTC	1 cut(s)	385
AspLEI	GCGC	1 cut(s)	713
AspS9I	GGNCC	4 cut(s)	757, 758, 980, 992
AsuHPI	GGTGA	4 cut(s)	470, 797, 863, 1119
AvaI	CYCGRG	1 cut(s)	1337
AxyI	CCTNAGG	1 cut(s)	284
BaeGI	GKGCMC	1 cut(s)	761
BanI	GGYRCC	1 cut(s)	1364
BanII	GRGCYC	4 cut(s)	123, 207, 761, 1290
BarI	GAAGNNNNNNTAC	2 cut(s)	431, 463
BbsI	GAAGAC	1 cut(s)	1230
Bbv12I	GWGCWC	2 cut(s)	123, 207
BbvI	GCAGC	4 cut(s)	196, 725, 823, 1439
BccI	CCATC	4 cut(s)	270, 758, 782, 1265
BceAI	ACGGC	1 cut(s)	600
BciT130I	CCWGG	1 cut(s)	1362
BclI	TGATCA	1 cut(s)	877
BcoDI	GTCTC	2 cut(s)	671, 1340
BfaI	CTAG	3 cut(s)	188, 803, 1459
BisI	GCNGC	4 cut(s)	185, 739, 837, 1453
BlsI	GCNGC	4 cut(s)	186, 740, 838, 1454
Bme1390I	CCNGG	1 cut(s)	1362
BmeRI	GACNNNNNGTC	1 cut(s)	1332
BmeT110I	CYCGRG	1 cut(s)	1337
BmgT120I	GGNCC	4 cut(s)	757, 758, 980, 992
BmiI	GGNNCC	2 cut(s)	759, 1366
BmrFI	CCNGG	1 cut(s)	1362
BmrI	ACTGGG	1 cut(s)	1286
BmsI	GCATC	2 cut(s)	783, 1186
BmuI	ACTGGG	1 cut(s)	1286
BpiI	GAAGAC	1 cut(s)	1230
BsaJI	CCNNGG	1 cut(s)	1075
BsaWI	WCCGGW	1 cut(s)	450
Bsc4I	CCNNNNNNNGG	5 cut(s)	57, 249, 367, 766, 989
Bse118I	RCCGGY	1 cut(s)	450
Bse1I	ACTGG	3 cut(s)	283, 1228, 1292
Bse21I	CCTNAGG	1 cut(s)	284
Bse3DI	GCAATG	2 cut(s)	312, 324
BseBI	CCWGG	1 cut(s)	1362
BseDI	CCNNGG	1 cut(s)	1075
BseGI	GGATG	2 cut(s)	441, 1201
BseLI	CCNNNNNNNGG	5 cut(s)	57, 249, 367, 766, 989
BseMI	GCAATG	2 cut(s)	312, 324
BseMII	CTCAG	1 cut(s)	549
BseNI	ACTGG	3 cut(s)	283, 1228, 1292
BseSI	GKGCMC	1 cut(s)	761
BseXI	GCAGC	4 cut(s)	196, 725, 823, 1439
BsgI	GTGCAG	2 cut(s)	724, 1438
Bsh1236I	CGCG	1 cut(s)	713
BshFI	GGCC	5 cut(s)	13, 282, 759, 981, 993
BshNI	GGYRCC	1 cut(s)	1364
BshTI	ACCGGT	1 cut(s)	450
BsiHKAI	GWGCWC	2 cut(s)	123, 207
BsiHKCI	CYCGRG	1 cut(s)	1337
BsiSI	CCGG	1 cut(s)	451
BslI	CCNNNNNNNGG	5 cut(s)	57, 249, 367, 766, 989
BsmAI	GTCTC	2 cut(s)	671, 1340
BsmI	GAATGC	1 cut(s)	20
BsnI	GGCC	5 cut(s)	13, 282, 759, 981, 993
BsoBI	CYCGRG	1 cut(s)	1337
Bsp120I	GGGCCC	1 cut(s)	757
Bsp1286I	GDGCHC	4 cut(s)	123, 207, 761, 1290
Bsp1407I	TGTACA	1 cut(s)	1321
Bsp143I	GATC	2 cut(s)	237, 877
BspACI	CCGC	5 cut(s)	211, 244, 456, 936, 1420
BspANI	GGCC	5 cut(s)	13, 282, 759, 981, 993
BspCNI	CTCAG	1 cut(s)	550
BspFNI	CGCG	1 cut(s)	713
BspLI	GGNNCC	2 cut(s)	759, 1366
BspQI	GCTCTTC	3 cut(s)	195, 559, 1292
BspT107I	GGYRCC	1 cut(s)	1364
BsrDI	GCAATG	2 cut(s)	312, 324
BsrFI	RCCGGY	1 cut(s)	450
BsrGI	TGTACA	1 cut(s)	1321
BsrI	ACTGG	3 cut(s)	283, 1228, 1292
BssAI	RCCGGY	1 cut(s)	450
BssECI	CCNNGG	1 cut(s)	1075
BssMI	GATC	2 cut(s)	237, 877
BssT1I	CCWWGG	1 cut(s)	1075
Bst2UI	CCWGG	1 cut(s)	1362
Bst4CI	ACNGT	2 cut(s)	682, 1191
Bst6I	CTCTTC	4 cut(s)	32, 195, 559, 1292
BstAPI	GCANNNNNTGC	1 cut(s)	160
BstAUI	TGTACA	1 cut(s)	1321
BstC8I	GCNNGC	3 cut(s)	772, 815, 969
BstDEI	CTNAG	2 cut(s)	284, 558
BstENI	CCTNNNNNAGG	1 cut(s)	365
BstF5I	GGATG	2 cut(s)	441, 1201
BstFNI	CGCG	1 cut(s)	713
BstHHI	GCGC	1 cut(s)	713
BstKTI	GATC	2 cut(s)	240, 880
BstMAI	GTCTC	2 cut(s)	671, 1340
BstMBI	GATC	2 cut(s)	237, 877
BstMWI	GCNNNNNNNGC	7 cut(s)	151, 160, 181, 314, 836, 935, 1274
BstNI	CCWGG	1 cut(s)	1362
BstSCI	CCNGG	1 cut(s)	1360
BstSLI	GKGCMC	1 cut(s)	761
BstUI	CGCG	1 cut(s)	713
BstV1I	GCAGC	4 cut(s)	196, 725, 823, 1439
BstV2I	GAAGAC	1 cut(s)	1230
Bsu36I	CCTNAGG	1 cut(s)	284
BsuRI	GGCC	5 cut(s)	13, 282, 759, 981, 993
BtsCI	GGATG	2 cut(s)	441, 1201
BtsI	GCAGTG	1 cut(s)	938
BtsIMutI	CAGTG	4 cut(s)	938, 1221, 1248, 1299
Cac8I	GCNNGC	3 cut(s)	772, 815, 969
CfoI	GCGC	1 cut(s)	713
Cfr10I	RCCGGY	1 cut(s)	450
Cfr13I	GGNCC	4 cut(s)	757, 758, 980, 992
Csp6I	GTAC	7 cut(s)	358, 448, 637, 689, 1177, 1322, 1428
CspAI	ACCGGT	1 cut(s)	450
CviAII	CATG	6 cut(s)	109, 155, 432, 883, 952, 1147
CviQI	GTAC	7 cut(s)	358, 448, 637, 689, 1177, 1322, 1428
DdeI	CTNAG	2 cut(s)	284, 558
DpnI	GATC	2 cut(s)	239, 879
DpnII	GATC	2 cut(s)	237, 877
DriI	GACNNNNNGTC	1 cut(s)	1332
Eam1104I	CTCTTC	4 cut(s)	32, 195, 559, 1292
Eam1105I	GACNNNNNGTC	1 cut(s)	1332
EarI	CTCTTC	4 cut(s)	32, 195, 559, 1292
Ecl136II	GAGCTC	2 cut(s)	121, 205
Eco130I	CCWWGG	1 cut(s)	1075
Eco24I	GRGCYC	4 cut(s)	123, 207, 761, 1290
Eco32I	GATATC	1 cut(s)	1023
Eco53kI	GAGCTC	2 cut(s)	121, 205
Eco57I	CTGAAG	1 cut(s)	1224
Eco81I	CCTNAGG	1 cut(s)	284
Eco88I	CYCGRG	1 cut(s)	1337
EcoICRI	GAGCTC	2 cut(s)	121, 205
EcoNI	CCTNNNNNAGG	1 cut(s)	365
EcoO109I	RGGNCCY	2 cut(s)	757, 758
EcoRI	GAATTC	1 cut(s)	1409
EcoRII	CCWGG	1 cut(s)	1360
EcoRV	GATATC	1 cut(s)	1023
EcoT14I	CCWWGG	1 cut(s)	1075
EcoT22I	ATGCAT	2 cut(s)	298, 1201
EcoT38I	GRGCYC	4 cut(s)	123, 207, 761, 1290
ErhI	CCWWGG	1 cut(s)	1075
FaeI	CATG	6 cut(s)	112, 158, 435, 886, 955, 1150
FatI	CATG	6 cut(s)	108, 154, 431, 882, 951, 1146
FauI	CCCGC	3 cut(s)	251, 943, 1413
FbaI	TGATCA	1 cut(s)	877
Fnu4HI	GCNGC	4 cut(s)	185, 739, 837, 1453
FokI	GGATG	2 cut(s)	448, 1208
FriOI	GRGCYC	4 cut(s)	123, 207, 761, 1290
Fsp4HI	GCNGC	4 cut(s)	185, 739, 837, 1453
FspBI	CTAG	3 cut(s)	188, 803, 1459
GlaI	GCGC	1 cut(s)	712
GluI	GCNGC	4 cut(s)	185, 739, 837, 1453
HaeIII	GGCC	5 cut(s)	13, 282, 759, 981, 993
HapII	CCGG	1 cut(s)	451
HhaI	GCGC	1 cut(s)	713
Hin1II	CATG	6 cut(s)	112, 158, 435, 886, 955, 1150
Hin6I	GCGC	1 cut(s)	711
HinP1I	GCGC	1 cut(s)	711
HindIII	AAGCTT	1 cut(s)	965
HinfI	GANTC	4 cut(s)	396, 746, 1097, 1235
HpaII	CCGG	1 cut(s)	451
HphI	GGTGA	4 cut(s)	470, 797, 863, 1119
Hpy166II	GTNNAC	1 cut(s)	1009
Hpy188I	TCNGA	2 cut(s)	141, 559
Hpy8I	GTNNAC	1 cut(s)	1009
HpyAV	CCTTC	5 cut(s)	62, 396, 499, 544, 1455
HpyCH4III	ACNGT	2 cut(s)	682, 1191
HpyCH4IV	ACGT	1 cut(s)	1115
HpyF10VI	GCNNNNNNNGC	7 cut(s)	151, 160, 181, 314, 836, 935, 1274
HpyF3I	CTNAG	2 cut(s)	284, 558
HpySE526I	ACGT	1 cut(s)	1115
Hsp92II	CATG	6 cut(s)	112, 158, 435, 886, 955, 1150
HspAI	GCGC	1 cut(s)	711
Ksp22I	TGATCA	1 cut(s)	877
Kzo9I	GATC	2 cut(s)	237, 877
LguI	GCTCTTC	3 cut(s)	195, 559, 1292
LmnI	GCTCC	4 cut(s)	210, 708, 1274, 1447
Lsp1109I	GCAGC	4 cut(s)	196, 725, 823, 1439
LweI	GCATC	2 cut(s)	783, 1186
MaeI	CTAG	3 cut(s)	188, 803, 1459
MaeII	ACGT	1 cut(s)	1115
MaeIII	GTNAC	4 cut(s)	164, 422, 851, 956
MalI	GATC	2 cut(s)	239, 879
MboI	GATC	2 cut(s)	237, 877
MfeI	CAATTG	1 cut(s)	1089
MhlI	GDGCHC	4 cut(s)	123, 207, 761, 1290
MlyI	GAGTC	2 cut(s)	755, 1244
MmeI	TCCRAC	2 cut(s)	626, 1173
Mph1103I	ATGCAT	2 cut(s)	298, 1201
MroXI	GAANNNNTTC	1 cut(s)	385
MseI	TTAA	1 cut(s)	372
MslI	CAYNNNNRTG	1 cut(s)	317
MspI	CCGG	1 cut(s)	451
MspR9I	CCNGG	1 cut(s)	1362
MunI	CAATTG	1 cut(s)	1089
Mva1269I	GAATGC	1 cut(s)	20
MvaI	CCWGG	1 cut(s)	1362
MvnI	CGCG	1 cut(s)	713
MwoI	GCNNNNNNNGC	7 cut(s)	151, 160, 181, 314, 836, 935, 1274
NdeII	GATC	2 cut(s)	237, 877
NlaIII	CATG	6 cut(s)	112, 158, 435, 886, 955, 1150
NlaIV	GGNNCC	2 cut(s)	759, 1366
NmeAIII	GCCGAG	1 cut(s)	1272
NmuCI	GTSAC	3 cut(s)	164, 422, 851
NsiI	ATGCAT	2 cut(s)	298, 1201
PciSI	GCTCTTC	3 cut(s)	195, 559, 1292
PctI	GAATGC	1 cut(s)	20
PdmI	GAANNNNTTC	1 cut(s)	385
PfeI	GAWTC	2 cut(s)	396, 1097
PinAI	ACCGGT	1 cut(s)	450
PkrI	GCNGC	4 cut(s)	186, 740, 838, 1454
PleI	GAGTC	2 cut(s)	754, 1243
PpsI	GAGTC	2 cut(s)	754, 1243
Psp124BI	GAGCTC	2 cut(s)	123, 207
Psp6I	CCWGG	1 cut(s)	1360
PspGI	CCWGG	1 cut(s)	1360
PspN4I	GGNNCC	2 cut(s)	759, 1366
PspOMI	GGGCCC	1 cut(s)	757
PspPI	GGNCC	4 cut(s)	757, 758, 980, 992
RsaI	GTAC	7 cut(s)	359, 449, 638, 690, 1178, 1323, 1429
RsaNI	GTAC	7 cut(s)	358, 448, 637, 689, 1177, 1322, 1428
RseI	CAYNNNNRTG	1 cut(s)	317
SacI	GAGCTC	2 cut(s)	123, 207
SapI	GCTCTTC	3 cut(s)	195, 559, 1292
SaqAI	TTAA	1 cut(s)	372
SatI	GCNGC	4 cut(s)	185, 739, 837, 1453
Sau3AI	GATC	2 cut(s)	237, 877
Sau96I	GGNCC	4 cut(s)	757, 758, 980, 992
SchI	GAGTC	2 cut(s)	755, 1244
ScrFI	CCNGG	1 cut(s)	1362
SduI	GDGCHC	4 cut(s)	123, 207, 761, 1290
SfaNI	GCATC	2 cut(s)	783, 1186
SmiMI	CAYNNNNRTG	1 cut(s)	317
SsiI	CCGC	5 cut(s)	211, 244, 456, 936, 1420
SspMI	CTAG	3 cut(s)	188, 803, 1459
SstI	GAGCTC	2 cut(s)	123, 207
StyD4I	CCNGG	1 cut(s)	1360
StyI	CCWWGG	1 cut(s)	1075
TaaI	ACNGT	2 cut(s)	682, 1191
TaiI	ACGT	1 cut(s)	1118
TaqI	TCGA	3 cut(s)	573, 753, 1413
TatI	WGTACW	1 cut(s)	1321
TfiI	GAWTC	2 cut(s)	396, 1097
Tru1I	TTAA	1 cut(s)	372
Tru9I	TTAA	1 cut(s)	372
TscAI	CASTG	4 cut(s)	945, 1228, 1248, 1299
TseFI	GTSAC	3 cut(s)	164, 422, 851
TseI	GCWGC	4 cut(s)	184, 738, 836, 1452
Tsp45I	GTSAC	3 cut(s)	164, 422, 851
TspDTI	ATGAA	9 cut(s)	17, 19, 62, 66, 102, 452, 503, 1071, 1250
TspRI	CASTG	4 cut(s)	945, 1228, 1248, 1299
XagI	CCTNNNNNAGG	1 cut(s)	365
XapI	RAATTY	2 cut(s)	1280, 1409
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1059
XmnI	GAANNNNTTC	1 cut(s)	385
XspI	CTAG	3 cut(s)	188, 803, 1459
Zsp2I	ATGCAT	2 cut(s)	298, 1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.