MD05G1250600.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006921
Physical Location & Seq
Forward (+)
38286759 .. 38289316
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1250600.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGATAAAGGTAACTCCAGCTTGTCCTTCCAATATGGTTGCTTATCTGATCCTTGTTTGGTCCAAGGCCATGGAGTACTGCTTACTGCCCACGATACGATTTCTCGTGTCGGTCCTCGACGGATTCTTCATACGTTTCTTATCAAAACCCGTGACGCTGCAGATCGAAGAGTACCACGGCACGAGCACGCGTAACAAAGTCTACGATGCGTTAGATCTTTACTTGAAAGACAAGATCAGCCCTTCAGCTCGGCTTCTCAAAGTTGCGCAAGCTCGTAAAGAGACTAATTTCACCGTCCAGTTTTCGAACAACCAAGAGTTTGATGACTCGTACGAAGGGATCCAGCTGAAATGGAAGTTCGTTTCCGTTTCCAAGAATTTCAAGTACAATCCAACTTCCTCTTCATCATATAACAGTGTTCCAGAGAAAAAGCAATACTTTGAACTTGTGTTTGACTTGCGACACAAGGAAAAGGTCATGAATTCTTACTTGCCTTATGTCATGGAGAGATTTAAGGCTATGAAAGAAGAAGGCAAAGTTGTGAAGCTGCATACACTTGTGAGGTTAGATGCTTTTAAGATCAGATGGGAGGCAATCAATCTCGAACACCCGGCAACGTTTGAGACGGTGGCCATGGACCCGGAGCTGAAGGCTGCGGTTGTGGAGGACTTGGACAGATTTGTGAAGAGGAAGGAGTTCTACCAAAGAGTGGGAAGAGCTTGGAAGAGAGGGTATCTTCTATATGGTCCTCCAGGAACTGGAAAGTCGTCCCTGATAGCTGCCATGGCTAATTACCTCAAGTTTGATGTCTTTGATTTGCAACTTACACATTTGAAAACGGATATGGAATTGAGAAAGGTGTTGCTGGCAACAACAAATAAGTCAATCCTTGTGATTGAGGACATCGATTGTAGTCAGCCGGCGGTTCAAGATCGGAAACAAAATAATTATGGCATAAAAAAGCCCGAGTTTGAAGGCAATCAGCTTACGCTATCCGGACTACTAAACTTCACAGATGGGCTGTGGTCTAGTTGTGGAGAAGAGAGAATCATTGTATTCACTACCAATCACAAGAGCAAGCTCGACTCTGCATTGCTTCGTTCTGGACGAATGGACATGCACATCCACATGTCGTTTTGCACGTACGAGGGCTTCAAAACGCTGGCTTGCAATTATCTCAAAATCTGTGGAACCCACCCTTTGTATGAGAAGATTGAAACCCTACTGCAGAAAACAAAGGCCACGCCTGCACAAGTGGCAGAGGAGCTAATGAAGAGTGAGGAGGCTGAAGTTGCACTCCAAGGTCTCATCACATTTCTAGAGGAAAAGAAGAACAATGTGAAAGATGAATACGAGGATGATGACGATGATAATGATCATAAACAATTAACATACTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

468

Amino Acids

53.98

Weight (kDa)

7.57

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 35 - 121 1.1e-18 Domain associated at C-terminal with AAA
AAA PF00004 245 - 377 1.3e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 380 - 447 3.4e-23 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccB7I CCANNNNNTGG 2 cut(s) 709, 758
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 190
AccIII TCCGGA 1 cut(s) 995
AciI CCGC 2 cut(s) 656, 923
AclI AACGTT 1 cut(s) 617
AclWI GGATC 3 cut(s) 43, 334, 347
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 2 cut(s) 376, 481
AcuI CTGAAG 3 cut(s) 228, 668, 1308
AfaI GTAC 5 cut(s) 77, 173, 332, 386, 1145
AfiI CCNNNNNNNGG 2 cut(s) 709, 758
AflIII ACRYGT 2 cut(s) 188, 1128
AgsI TTSAA 8 cut(s) 226, 382, 443, 835, 929, 974, 1156, 1217
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 385, 417
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 3 cut(s) 275, 617, 1310
AlwI GGATC 3 cut(s) 43, 334, 347
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 965
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 3 cut(s) 66, 630, 1239
ApeKI GCWGC 4 cut(s) 157, 547, 653, 779
ApoI RAATTY 2 cut(s) 376, 481
Asp700I GAANNNNTTC 1 cut(s) 695
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 4 cut(s) 60, 112, 637, 746
AsuC2I CCSGG 2 cut(s) 611, 641
AsuHPI GGTGA 1 cut(s) 283
AsuII TTCGAA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 965
AvaII GGWCC 4 cut(s) 60, 112, 637, 746
BalI TGGCCA 1 cut(s) 632
BamHI GGATCC 1 cut(s) 339
BarI GAAGNNNNNNTAC 4 cut(s) 683, 715, 1136, 1168
BauI CACGAG 2 cut(s) 104, 181
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 144, 534, 640, 766
BccI CCATC 2 cut(s) 579, 1010
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 753
BclI TGATCA 1 cut(s) 1375
BcnI CCSGG 2 cut(s) 611, 641
BcoDI GTCTC 3 cut(s) 275, 617, 1310
BfaI CTAG 2 cut(s) 1029, 1319
BfmI CTRYAG 2 cut(s) 158, 1226
BglII AGATCT 1 cut(s) 214
BisI GCNGC 4 cut(s) 158, 548, 654, 780
BlsI GCNGC 4 cut(s) 159, 549, 655, 781
BmcAI AGTACT 1 cut(s) 77
Bme1390I CCNGG 3 cut(s) 611, 641, 753
Bme18I GGWCC 4 cut(s) 60, 112, 637, 746
BmeT110I CYCGRG 1 cut(s) 965
BmgT120I GGNCC 4 cut(s) 60, 112, 637, 746
BmiI GGNNCC 3 cut(s) 341, 639, 1192
BmrFI CCNGG 3 cut(s) 611, 641, 753
BmsI GCATC 2 cut(s) 196, 559
BpmI CTGGAG 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 2 cut(s) 611, 641
Bsa29I ATCGAT 1 cut(s) 906
BsaAI YACGTR 1 cut(s) 1143
BsaBI GATNNNNATC 1 cut(s) 1374
BsaI GGTCTC 1 cut(s) 1310
BsaJI CCNNGG 6 cut(s) 63, 69, 175, 633, 783, 1300
BsaWI WCCGGW 1 cut(s) 995
Bsc4I CCNNNNNNNGG 2 cut(s) 709, 758
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 2 cut(s) 298, 763
Bse3DI GCAATG 1 cut(s) 1091
Bse8I GATNNNNATC 1 cut(s) 1374
BseAI TCCGGA 1 cut(s) 995
BseBI CCWGG 1 cut(s) 753
BseCI ATCGAT 1 cut(s) 906
BseDI CCNNGG 6 cut(s) 63, 69, 175, 633, 783, 1300
BseGI GGATG 2 cut(s) 1122, 1363
BseJI GATNNNNATC 1 cut(s) 1374
BseLI CCNNNNNNNGG 2 cut(s) 709, 758
BseMI GCAATG 1 cut(s) 1091
BseNI ACTGG 2 cut(s) 298, 763
BseRI GAGGAG 2 cut(s) 1277, 1295
BseXI GCAGC 4 cut(s) 144, 534, 640, 766
BsgI GTGCAG 1 cut(s) 1233
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 68, 632, 1241
BshVI ATCGAT 1 cut(s) 906
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 4 cut(s) 611, 641, 920, 996
BsiWI CGTACG 2 cut(s) 330, 1143
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 2 cut(s) 709, 758
BsmAI GTCTC 3 cut(s) 275, 617, 1310
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 754
BsnI GGCC 3 cut(s) 68, 632, 1241
Bso31I GGTCTC 1 cut(s) 1310
BsoBI CYCGRG 1 cut(s) 965
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 188
Bsp13I TCCGGA 1 cut(s) 995
Bsp143I GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
Bsp19I CCATGG 3 cut(s) 69, 633, 783
BspACI CCGC 2 cut(s) 656, 923
BspANI GGCC 3 cut(s) 68, 632, 1241
BspDI ATCGAT 1 cut(s) 906
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 341, 639, 1192
BspMAI CTGCAG 2 cut(s) 162, 1230
BspPI GGATC 3 cut(s) 43, 334, 347
BspQI GCTCTTC 1 cut(s) 709
BspT104I TTCGAA 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 1310
BsrDI GCAATG 1 cut(s) 1091
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 2 cut(s) 298, 763
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 6 cut(s) 63, 69, 175, 633, 783, 1300
BssMI GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
BssSI CACGAG 2 cut(s) 104, 181
BssT1I CCWWGG 5 cut(s) 63, 69, 633, 783, 1300
Bst2BI CACGAG 2 cut(s) 104, 181
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 3 cut(s) 295, 416, 628
Bst6I CTCTTC 7 cut(s) 162, 406, 680, 709, 719, 1035, 1268
BstBAI YACGTR 1 cut(s) 1143
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 8 cut(s) 188, 270, 867, 921, 1079, 1164, 1168, 1248
BstDSI CCRYGG 4 cut(s) 69, 175, 633, 783
BstF5I GGATG 2 cut(s) 1122, 1363
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 8 cut(s) 51, 165, 217, 237, 342, 582, 934, 1378
BstMAI GTCTC 3 cut(s) 275, 617, 1310
BstMBI GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
BstMWI GCNNNNNNNGC 4 cut(s) 785, 1247, 1256, 1292
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 2 cut(s) 1120, 1132
BstSCI CCNGG 3 cut(s) 609, 639, 751
BstSFI CTRYAG 2 cut(s) 158, 1226
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 4 cut(s) 144, 534, 640, 766
BstX2I RGATCY 2 cut(s) 214, 339
BstXI CCANNNNNNTGG 1 cut(s) 70
BstYI RGATCY 2 cut(s) 214, 339
Bsu15I ATCGAT 1 cut(s) 906
BsuRI GGCC 3 cut(s) 68, 632, 1241
BsuTUI ATCGAT 1 cut(s) 906
BtgI CCRYGG 4 cut(s) 69, 175, 633, 783
BtsCI GGATG 2 cut(s) 1122, 1363
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 8 cut(s) 188, 270, 867, 921, 1079, 1164, 1168, 1248
CaiI CAGNNNCTG 1 cut(s) 758
CciI TCATGA 1 cut(s) 477
CfoI GCGC 1 cut(s) 268
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 4 cut(s) 60, 112, 637, 746
ClaI ATCGAT 1 cut(s) 906
CseI GACGC 1 cut(s) 163
Csp6I GTAC 5 cut(s) 76, 172, 331, 385, 1144
CspCI CAANNNNNGTGG 2 cut(s) 1168, 1203
CviAII CATG 7 cut(s) 70, 478, 502, 634, 784, 1117, 1129
CviQI GTAC 5 cut(s) 76, 172, 331, 385, 1144
DpnI GATC 8 cut(s) 50, 164, 216, 236, 341, 581, 933, 1377
DpnII GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 7 cut(s) 162, 406, 680, 709, 719, 1035, 1268
EarI CTCTTC 7 cut(s) 162, 406, 680, 709, 719, 1035, 1268
Eco130I CCWWGG 5 cut(s) 63, 69, 633, 783, 1300
Eco31I GGTCTC 1 cut(s) 1310
Eco47I GGWCC 4 cut(s) 60, 112, 637, 746
Eco57I CTGAAG 3 cut(s) 228, 668, 1308
Eco88I CYCGRG 1 cut(s) 965
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 751
EcoT14I CCWWGG 5 cut(s) 63, 69, 633, 783, 1300
ErhI CCWWGG 5 cut(s) 63, 69, 633, 783, 1300
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 7 cut(s) 73, 481, 505, 637, 787, 1120, 1132
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 1 cut(s) 754
FatI CATG 7 cut(s) 69, 477, 501, 633, 783, 1116, 1128
FbaI TGATCA 1 cut(s) 1375
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 158, 548, 654, 780
FokI GGATG 2 cut(s) 1109, 1370
Fsp4HI GCNGC 4 cut(s) 158, 548, 654, 780
FspBI CTAG 2 cut(s) 1029, 1319
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 1 cut(s) 267
GluI GCNGC 4 cut(s) 158, 548, 654, 780
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 3 cut(s) 68, 632, 1241
HapII CCGG 4 cut(s) 611, 641, 920, 996
HgaI GACGC 1 cut(s) 163
HhaI GCGC 1 cut(s) 268
Hin1II CATG 7 cut(s) 73, 481, 505, 637, 787, 1120, 1132
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 4 cut(s) 123, 326, 1047, 1085
HpaII CCGG 4 cut(s) 611, 641, 920, 996
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 3 cut(s) 48, 584, 936
Hpy188III TCNNGA 7 cut(s) 422, 478, 602, 929, 996, 1104, 1319
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 7 cut(s) 36, 252, 329, 524, 643, 685, 968
HpyCH4III ACNGT 3 cut(s) 295, 416, 628
HpyCH4IV ACGT 3 cut(s) 133, 617, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 785, 1247, 1256, 1292
HpySE526I ACGT 3 cut(s) 133, 617, 1142
Hsp92II CATG 7 cut(s) 73, 481, 505, 637, 787, 1120, 1132
HspAI GCGC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 995
KroI GCCGGC 1 cut(s) 919
KroNI GCCGGC 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 1375
Kzo9I GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
LguI GCTCTTC 1 cut(s) 709
LmnI GCTCC 2 cut(s) 643, 1264
Lsp1109I GCAGC 4 cut(s) 144, 534, 640, 766
LweI GCATC 2 cut(s) 196, 559
MaeI CTAG 2 cut(s) 1029, 1319
MaeII ACGT 3 cut(s) 133, 617, 1142
MaeIII GTNAC 3 cut(s) 10, 151, 191
MalI GATC 8 cut(s) 50, 164, 216, 236, 341, 581, 933, 1377
MboI GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
MflI RGATCY 2 cut(s) 214, 339
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 8 cut(s) 286, 376, 481, 790, 848, 946, 1171, 1385
MluI ACGCGT 1 cut(s) 188
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 2 cut(s) 320, 1079
MmeI TCCRAC 1 cut(s) 416
Mox20I TGGCCA 1 cut(s) 632
MroI TCCGGA 1 cut(s) 995
MroNI GCCGGC 1 cut(s) 919
MroXI GAANNNNTTC 1 cut(s) 695
MscI TGGCCA 1 cut(s) 632
MseI TTAA 3 cut(s) 513, 576, 1388
MslI CAYNNNNRTG 1 cut(s) 1127
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 4 cut(s) 611, 641, 920, 996
MspR9I CCNGG 3 cut(s) 611, 641, 753
MvaI CCWGG 1 cut(s) 753
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 4 cut(s) 785, 1247, 1256, 1292
NaeI GCCGGC 1 cut(s) 921
NciI CCSGG 2 cut(s) 611, 641
NcoI CCATGG 3 cut(s) 69, 633, 783
NdeII GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
NgoMIV GCCGGC 1 cut(s) 919
NlaIII CATG 7 cut(s) 73, 481, 505, 637, 787, 1120, 1132
NlaIV GGNNCC 3 cut(s) 341, 639, 1192
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 151
NsbI TGCGCA 1 cut(s) 267
NspI RCATGY 2 cut(s) 1120, 1132
NspV TTCGAA 1 cut(s) 305
PagI TCATGA 1 cut(s) 477
PciI ACATGT 1 cut(s) 1128
PciSI GCTCTTC 1 cut(s) 709
PcsI WCGNNNNNNNCGW 2 cut(s) 1105, 1139
PdiI GCCGGC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 695
PfeI GAWTC 2 cut(s) 123, 1047
Pfl23II CGTACG 2 cut(s) 330, 1143
PflMI CCANNNNNTGG 2 cut(s) 709, 758
PfoI TCCNGGA 1 cut(s) 751
PkrI GCNGC 4 cut(s) 159, 549, 655, 781
PleI GAGTC 2 cut(s) 320, 1079
PpsI GAGTC 2 cut(s) 320, 1079
Ppu21I YACGTR 1 cut(s) 1143
PscI ACATGT 1 cut(s) 1128
Psp1406I AACGTT 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 751
PspLI CGTACG 2 cut(s) 330, 1143
PspN4I GGNNCC 3 cut(s) 341, 639, 1192
PspPI GGNCC 4 cut(s) 60, 112, 637, 746
PstI CTGCAG 2 cut(s) 162, 1230
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 2 cut(s) 214, 339
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 5 cut(s) 77, 173, 332, 386, 1145
RsaNI GTAC 5 cut(s) 76, 172, 331, 385, 1144
RseI CAYNNNNRTG 1 cut(s) 1127
SapI GCTCTTC 1 cut(s) 709
SaqAI TTAA 3 cut(s) 513, 576, 1388
SatI GCNGC 4 cut(s) 158, 548, 654, 780
Sau3AI GATC 8 cut(s) 48, 162, 214, 234, 339, 579, 931, 1375
Sau96I GGNCC 4 cut(s) 60, 112, 637, 746
ScaI AGTACT 1 cut(s) 77
SchI GAGTC 2 cut(s) 320, 1079
ScrFI CCNGG 3 cut(s) 611, 641, 753
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 2 cut(s) 196, 559
SfcI CTRYAG 2 cut(s) 158, 1226
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 4 cut(s) 60, 112, 637, 746
SmiMI CAYNNNNRTG 1 cut(s) 1127
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 8 cut(s) 286, 376, 481, 790, 848, 946, 1171, 1385
SsiI CCGC 2 cut(s) 656, 923
SspMI CTAG 2 cut(s) 1029, 1319
StyD4I CCNGG 3 cut(s) 609, 639, 751
StyI CCWWGG 5 cut(s) 63, 69, 633, 783, 1300
TaaI ACNGT 3 cut(s) 295, 416, 628
TaiI ACGT 3 cut(s) 136, 620, 1145
TaqI TCGA 6 cut(s) 117, 165, 305, 603, 906, 1083
TaqII GACCGA 1 cut(s) 100
TasI AATT 8 cut(s) 286, 376, 481, 790, 848, 946, 1171, 1385
TatI WGTACW 2 cut(s) 75, 384
TfiI GAWTC 2 cut(s) 123, 1047
Tru1I TTAA 3 cut(s) 513, 576, 1388
Tru9I TTAA 3 cut(s) 513, 576, 1388
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 157, 547, 653, 779
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 6 cut(s) 118, 393, 494, 536, 1286, 1362
TspGWI ACGGA 3 cut(s) 135, 355, 854
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 2 cut(s) 709, 758
VpaK11BI GGWCC 4 cut(s) 60, 112, 637, 746
XapI RAATTY 2 cut(s) 376, 481
XbaI TCTAGA 1 cut(s) 1318
XceI RCATGY 2 cut(s) 1120, 1132
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 695
XspI CTAG 2 cut(s) 1029, 1319
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.