Rorug05G0058300

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005230
Physical Location & Seq
Reverse (-)
5078984 .. 5081003
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0058300.1

Sequence Viewer

Length: 726 bp
ATGGAAATGCAACAATACTGTGCACATGAATGGAAGTCCATCTTAGGTGCAACAGCCGATTCTGAAAATTCTAAAGGCGGCTTTGACTGCAACATCTGCTTAGACTTTGCACACGAGCCAGTGGTCACCCTCTGTGGCCATCTGTACTGCTGGCCTTGCATCTACAAATGGCTTCACGTCCAGAGCGCCTCCCTTGCTTCTGATGAGTGCCCTCAGTGCCCTGTTTGCAAGACCGAGATATCACACACCACCATGGTCCCCCTTTATGGCCGTGGCCAAACAACTTCAGAAGCTGAGCTCAAAGGAAAGGCACCCTCCCGGGGAATAATAATACCTCCCAGACCATCAGCATGGGATGCACAAGCTCTTCTATCTAACTCACCTCGTAATGGTCAGCAACTTCCATATCGTAACCCTTATCAGAACCAACAATATAGCTCTCATCCCTACAGCAGTTATGAGGAACAATCTTCCTCACCACTGCTTAGCCTCGGAGGCACAACGTTAGCTGGTATCAACCATCCGATGGCTGGTCTATTCGGGGAGATGGTTTATGCAAGGGTGTTCGGAAACTCTGAGAGCTTATACGCGTATCCAAATTCGTACCACCTAACGGGCAGTAGTAGTCCAAGGTTGAGAAGGCACGAGATGCAGGCAGAAAAGTCGCTAAACAGAATTTCTTTCTTCCTCTTCTGTTGCTTGCTTTTGTGCCTTATTGTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

241

Amino Acids

26.87

Weight (kDa)

6.49

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 29 - 76 8.1e-07 Ring finger domain
zf-C3HC4 PF00097 30 - 76 1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 30 - 73 1.5e-06 Zinc finger, C3HC4 type (RING finger)
RING_XB3-XBAT31 PF24921 30 - 81 4.2e-07 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX PF13445 30 - 59 3.2e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 526
AccII CGCG 1 cut(s) 590
AciI CCGC 1 cut(s) 78
AclI AACGTT 1 cut(s) 503
AcoI YGGCCR 3 cut(s) 136, 268, 274
AcsI RAATTY 3 cut(s) 67, 598, 675
AcuI CTGAAG 1 cut(s) 270
AfaI GTAC 2 cut(s) 146, 605
AfiI CCNNNNNNNGG 6 cut(s) 266, 320, 389, 526, 530, 613
AflIII ACRYGT 1 cut(s) 588
AjiI CACGTC 1 cut(s) 178
AluBI AGCT 6 cut(s) 293, 298, 365, 438, 509, 582
AluI AGCT 6 cut(s) 293, 298, 365, 438, 509, 582
Alw21I GWGCWC 2 cut(s) 25, 300
Alw44I GTGCAC 1 cut(s) 21
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 4 cut(s) 136, 152, 268, 274
ApaLI GTGCAC 1 cut(s) 21
ApoI RAATTY 3 cut(s) 67, 598, 675
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 2 cut(s) 319, 320
AsuHPI GGTGA 3 cut(s) 118, 372, 468
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 3 cut(s) 25, 212, 221
BalI TGGCCA 2 cut(s) 138, 276
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 300
BauI CACGAG 2 cut(s) 113, 644
Bbv12I GWGCWC 2 cut(s) 25, 300
BccI CCATC 6 cut(s) 47, 147, 352, 520, 528, 541
BceAI ACGGC 1 cut(s) 255
BcgI CGANNNNNNTGC 2 cut(s) 645, 679
BciVI GTATCC 1 cut(s) 603
BcnI CCSGG 2 cut(s) 319, 320
BfmI CTRYAG 1 cut(s) 448
BfoI RGCGCY 1 cut(s) 189
BfuI GTATCC 1 cut(s) 603
BglI GCCNNNNNGGC 1 cut(s) 495
BisI GCNGC 1 cut(s) 79
BlpI GCTNAGC 2 cut(s) 294, 485
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 2 cut(s) 319, 320
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 258, 312
BmrFI CCNGG 2 cut(s) 319, 320
BmsI GCATC 3 cut(s) 168, 346, 639
Bpu1102I GCTNAGC 2 cut(s) 294, 485
BpuMI CCSGG 2 cut(s) 319, 320
BsaJI CCNNGG 6 cut(s) 252, 271, 318, 319, 490, 629
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 320, 389, 526, 530, 613
Bse1I ACTGG 1 cut(s) 119
BseDI CCNNGG 6 cut(s) 252, 271, 318, 319, 490, 629
BseGI GGATG 3 cut(s) 361, 442, 520
BseLI CCNNNNNNNGG 6 cut(s) 266, 320, 389, 526, 530, 613
BseMII CTCAG 3 cut(s) 227, 285, 567
BseNI ACTGG 1 cut(s) 119
BseSI GKGCMC 3 cut(s) 25, 212, 221
Bsh1236I CGCG 1 cut(s) 590
BshFI GGCC 4 cut(s) 138, 154, 270, 276
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 25, 300
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 6 cut(s) 266, 320, 389, 526, 530, 613
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 4 cut(s) 138, 154, 270, 276
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 4 cut(s) 25, 212, 221, 300
Bsp1720I GCTNAGC 2 cut(s) 294, 485
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 1 cut(s) 78
BspANI GGCC 4 cut(s) 138, 154, 270, 276
BspCNI CTCAG 3 cut(s) 226, 286, 568
BspFNI CGCG 1 cut(s) 590
BspLI GGNNCC 2 cut(s) 258, 312
BspQI GCTCTTC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 310
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 6 cut(s) 252, 271, 318, 319, 490, 629
BssSI CACGAG 2 cut(s) 113, 644
BssT1I CCWWGG 2 cut(s) 252, 629
Bst2BI CACGAG 2 cut(s) 113, 644
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 2 cut(s) 372, 695
BstAPI GCANNNNNTGC 3 cut(s) 96, 356, 649
BstC8I GCNNGC 3 cut(s) 152, 654, 701
BstDEI CTNAG 6 cut(s) 43, 100, 213, 294, 485, 576
BstDSI CCRYGG 2 cut(s) 252, 271
BstEII GGTNACC 1 cut(s) 124
BstF5I GGATG 3 cut(s) 361, 442, 520
BstFNI CGCG 1 cut(s) 590
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 1 cut(s) 188
BstMWI GCNNNNNNNGC 9 cut(s) 87, 96, 156, 194, 216, 225, 356, 495, 649
BstPI GGTNACC 1 cut(s) 124
BstSCI CCNGG 2 cut(s) 317, 318
BstSFI CTRYAG 1 cut(s) 448
BstSLI GKGCMC 3 cut(s) 25, 212, 221
BstUI CGCG 1 cut(s) 590
BstXI CCANNNNNNTGG 1 cut(s) 351
BsuI GTATCC 1 cut(s) 603
BsuRI GGCC 4 cut(s) 138, 154, 270, 276
BtgI CCRYGG 2 cut(s) 252, 271
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 3 cut(s) 361, 442, 520
BtsI GCAGTG 1 cut(s) 479
BtsIMutI CAGTG 3 cut(s) 126, 221, 479
Cac8I GCNNGC 3 cut(s) 152, 654, 701
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 256
Cfr9I CCCGGG 1 cut(s) 318
Csp6I GTAC 2 cut(s) 145, 604
CviAII CATG 3 cut(s) 26, 253, 351
CviQI GTAC 2 cut(s) 145, 604
DdeI CTNAG 6 cut(s) 43, 100, 213, 294, 485, 576
EaeI YGGCCR 3 cut(s) 136, 268, 274
Eam1104I CTCTTC 2 cut(s) 372, 695
EarI CTCTTC 2 cut(s) 372, 695
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 2 cut(s) 252, 629
Eco24I GRGCYC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 318
Eco91I GGTNACC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 298
EcoO65I GGTNACC 1 cut(s) 124
EcoRV GATATC 1 cut(s) 240
EcoT14I CCWWGG 2 cut(s) 252, 629
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 2 cut(s) 252, 629
FaeI CATG 3 cut(s) 29, 256, 354
FaiI YATR 9 cut(s) 27, 254, 267, 352, 406, 435, 459, 555, 586
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 242
FatI CATG 3 cut(s) 25, 252, 350
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 3 cut(s) 368, 429, 507
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 79
GlaI GCGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 79
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 4 cut(s) 138, 154, 270, 276
HapII CCGG 1 cut(s) 319
HhaI GCGC 1 cut(s) 188
Hin1II CATG 3 cut(s) 29, 256, 354
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 319
HphI GGTGA 3 cut(s) 118, 372, 468
Hpy166II GTNNAC 1 cut(s) 23
Hpy188I TCNGA 9 cut(s) 64, 202, 289, 423, 494, 525, 569, 577, 725
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 633
HpyCH4III ACNGT 1 cut(s) 20
HpyCH4IV ACGT 2 cut(s) 177, 503
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 96, 156, 194, 216, 225, 356, 495, 649
HpyF3I CTNAG 6 cut(s) 43, 100, 213, 294, 485, 576
HpySE526I ACGT 2 cut(s) 177, 503
Hsp92II CATG 3 cut(s) 29, 256, 354
HspAI GCGC 1 cut(s) 186
LguI GCTCTTC 1 cut(s) 372
LpnPI CCDG 9 cut(s) 132, 136, 194, 234, 332, 352, 495, 516, 638
LweI GCATC 3 cut(s) 168, 346, 639
MaeII ACGT 2 cut(s) 177, 503
MaeIII GTNAC 2 cut(s) 124, 410
MboII GAAGA 4 cut(s) 359, 462, 676, 682
MhlI GDGCHC 4 cut(s) 25, 212, 221, 300
MlsI TGGCCA 2 cut(s) 138, 276
MluCI AATT 3 cut(s) 67, 598, 675
MluI ACGCGT 1 cut(s) 588
MluNI TGGCCA 2 cut(s) 138, 276
Mox20I TGGCCA 2 cut(s) 138, 276
MscI TGGCCA 2 cut(s) 138, 276
MslI CAYNNNNRTG 3 cut(s) 28, 251, 349
Msp20I TGGCCA 2 cut(s) 138, 276
MspI CCGG 1 cut(s) 319
MspR9I CCNGG 2 cut(s) 319, 320
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 9 cut(s) 87, 96, 156, 194, 216, 225, 356, 495, 649
NciI CCSGG 2 cut(s) 319, 320
NcoI CCATGG 1 cut(s) 252
NlaIII CATG 3 cut(s) 29, 256, 354
NlaIV GGNNCC 2 cut(s) 258, 312
NmuCI GTSAC 1 cut(s) 124
PciSI GCTCTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 526
PkrI GCNGC 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 503
PspEI GGTNACC 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 258, 312
PspPI GGNCC 1 cut(s) 256
PstNI CAGNNNCTG 1 cut(s) 293
RsaI GTAC 2 cut(s) 146, 605
RsaNI GTAC 2 cut(s) 145, 604
RseI CAYNNNNRTG 3 cut(s) 28, 251, 349
SacI GAGCTC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 372
SatI GCNGC 1 cut(s) 79
Sau96I GGNCC 1 cut(s) 256
ScrFI CCNGG 2 cut(s) 319, 320
SduI GDGCHC 4 cut(s) 25, 212, 221, 300
SfaNI GCATC 3 cut(s) 168, 346, 639
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 1 cut(s) 256
SmaI CCCGGG 1 cut(s) 320
SmiMI CAYNNNNRTG 3 cut(s) 28, 251, 349
Sse9I AATT 3 cut(s) 67, 598, 675
SsiI CCGC 1 cut(s) 78
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 2 cut(s) 317, 318
StyI CCWWGG 2 cut(s) 252, 629
TaaI ACNGT 1 cut(s) 20
TaiI ACGT 2 cut(s) 180, 506
TaqII GACCGA 1 cut(s) 248
TasI AATT 3 cut(s) 67, 598, 675
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 59
TscAI CASTG 3 cut(s) 126, 221, 486
TseFI GTSAC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 42
TspMI CCCGGG 1 cut(s) 318
TspRI CASTG 3 cut(s) 126, 221, 486
Van91I CCANNNNNTGG 1 cut(s) 526
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 3 cut(s) 67, 598, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 527
XmaI CCCGGG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.