Rh1AG268700

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005230
Physical Location & Seq
Forward (+)
49582798 .. 49585182
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG268700.1

Sequence Viewer

Length: 1452 bp
ATGGGATTGTTGAGAAGAAATCCCATGAAAACTCAAGTGATGAAATCAGTGTTAATAGTTGTTGTGGTGGCCTTAATACTAAGAGGGGGAGAGAGCAGAGGAGTAGCCAAGGCCCCCATAAAATCTGAGTCCTTTGAGTACTCTGCTATGAATTGCCGAGCTCACAGTGCTTCACTGACCGAGTTTGGAGGAGTTGGAGATGGAAAGACATCAAATACAAAGGCTTTTCAGACTGCAATCAGTAAGCTGAGCCAGTTTGCAACAGATGGAGGTGCTCAGCTTTTTGTTCCAGCTGGGAAATGGTTGACAGGAAGCTTCAGCCTCACCAGCCATTTCACTCTTTATCTTCACAAGGATGCTGTTCTTCTTGCTTCTCAGGACTTGAATGAGTGGCCTGTGCTTAAGGCCCTTCCATCTTATGGCAGAGGAAGAGATGCAGCAGCTGGAAGGTACTCCAGTCTCATATTTGGAACAAATCTTACTGATGTCATTGTTACAGGAGACAATGGCACAATTGATGGTCAAGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTACCTGATTGAGATCATGTTCTCAAGTGATATCCAGATCTCAGATCTCACCCTCCTAAATTCTCCATCATGGAATGTTCATCCTGTCTATAGCAGTAACATTCTTGTGCAAGGGATTACTATCATTGCTCCAATTTCGTCTCCAAACACTGATGGGATCAATCCAGATTCTTGCACAAATACTAGAATTGAAGACTGTTACATAGTCTCTGGTGATGATTGTGTAGCTGTTAAGAGTGGTTGGGATGAGTATGGCATATCATTTGGAATGCCTACAAAACAACTAGTGATCAGACGGCTCACATGCATTTCACCCTACAGTGCAACCATCGCATTGGGCAGCGAAATGTCAGGTGGGATTCAAGATGTCAGAGCCGAAGACATCGTGGCCATCAATACCGAATCAGCCGTCAGGATCAAGACAGCTGTAGGGAGAGGAGGGTATGTGAAAGACATATATGTAAGGAGAATGACACTGCACACAATGAAATGGGCATTTTGGATGACTGGTAATTATGGGTCACATGCTGATGGTAACTATGACAAAAATGCCTTTCCTGAGATTACTGGGATTAACTATAGAGACGTGGTGGCTGAAAATGTAACCATGGCTGCAAGATTGGAAGGCATTGCCGACCATCCATTTACCGGGATTTGCATGTCCAATGTGACCATTGGATTGGCAAAGAAGGCAAAGAAGCAGCCATGGACATGTACTGATATTCAGGGGATCACAAGCGGTGTGACGCCAACGCCCTGTGGTCTGCTGCCTGATCAAGGAACTGCTAAACCTGATGGTTGTGATTTTCCAGCAGATGATATACCGATCGACATGGTAGAGATTAAAAAGTGTACTTATAGGAGTAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

483

Amino Acids

52.55

Weight (kDa)

7.53

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 152 - 435 5.6e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 419
AciI CCGC 2 cut(s) 574, 1317
AclWI GGATC 3 cut(s) 743, 1001, 1316
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 1325
AfaI GTAC 4 cut(s) 140, 452, 1294, 1432
AfiI CCNNNNNNNGG 3 cut(s) 419, 1226, 1355
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 1289
AgsI TTSAA 3 cut(s) 385, 770, 941
AhlI ACTAGT 1 cut(s) 862
AjiI CACGTC 1 cut(s) 1165
AluBI AGCT 9 cut(s) 161, 247, 280, 293, 315, 443, 562, 806, 1004
AluI AGCT 9 cut(s) 161, 247, 280, 293, 315, 443, 562, 806, 1004
Alw21I GWGCWC 2 cut(s) 163, 277
Alw26I GTCTC 5 cut(s) 464, 495, 723, 790, 1155
AlwI GGATC 3 cut(s) 743, 1001, 1316
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 5 cut(s) 69, 111, 392, 405, 966
ApeKI GCWGC 6 cut(s) 437, 440, 918, 1190, 1279, 1345
ApoI RAATTY 1 cut(s) 637
ArsI GACNNNNNNTTYG 2 cut(s) 972, 1004
AspS9I GGNCC 2 cut(s) 112, 406
AsuC2I CCSGG 1 cut(s) 1228
AsuHPI GGTGA 5 cut(s) 316, 539, 619, 803, 882
BaeI ACNNNNGTAYC 2 cut(s) 442, 475
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 2 cut(s) 777, 963
Bbv12I GWGCWC 2 cut(s) 163, 277
BbvI GCAGC 6 cut(s) 449, 452, 930, 1177, 1291, 1332
BceAI ACGGC 2 cut(s) 890, 971
BclI TGATCA 2 cut(s) 867, 1351
BcnI CCSGG 1 cut(s) 1228
BcoDI GTCTC 5 cut(s) 464, 495, 723, 790, 1155
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 2 cut(s) 762, 863
BfmI CTRYAG 4 cut(s) 667, 895, 1005, 1156
BfrI CTTAAG 1 cut(s) 401
BglII AGATCT 2 cut(s) 615, 622
BisI GCNGC 6 cut(s) 438, 441, 919, 1191, 1280, 1346
BlpI GCTNAGC 2 cut(s) 248, 276
BlsI GCNGC 6 cut(s) 439, 442, 920, 1192, 1281, 1347
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 1228
BmgBI CACGTC 1 cut(s) 1165
BmgT120I GGNCC 2 cut(s) 112, 406
BmiI GGNNCC 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 1228
BmrI ACTGGG 1 cut(s) 1155
BmsI GCATC 2 cut(s) 346, 424
BmuI ACTGGG 1 cut(s) 1155
BpiI GAAGAC 2 cut(s) 777, 963
BpmI CTGGAG 1 cut(s) 439
Bpu1102I GCTNAGC 2 cut(s) 248, 276
BpuEI CTTGAG 2 cut(s) 18, 586
BpuMI CCSGG 1 cut(s) 1228
BsaBI GATNNNNATC 2 cut(s) 590, 698
BsaHI GRCGYC 1 cut(s) 1325
BsaJI CCNNGG 3 cut(s) 108, 1185, 1283
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 1226, 1355
Bse1I ACTGG 4 cut(s) 253, 456, 1090, 1150
Bse3DI GCAATG 2 cut(s) 702, 1206
Bse8I GATNNNNATC 2 cut(s) 590, 698
BseDI CCNNGG 3 cut(s) 108, 1185, 1283
BseGI GGATG 5 cut(s) 361, 658, 829, 1086, 1216
BseJI GATNNNNATC 2 cut(s) 590, 698
BseLI CCNNNNNNNGG 3 cut(s) 419, 1226, 1355
BseMI GCAATG 2 cut(s) 702, 1206
BseMII CTCAG 6 cut(s) 117, 239, 290, 389, 633, 1128
BseNI ACTGG 4 cut(s) 253, 456, 1090, 1150
BseRI GAGGAG 3 cut(s) 114, 204, 1029
BseXI GCAGC 6 cut(s) 449, 452, 930, 1177, 1291, 1332
BseYI CCCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 1040
Bsh1285I CGRYCG 1 cut(s) 1407
BshFI GGCC 5 cut(s) 71, 113, 394, 407, 968
BsiEI CGRYCG 1 cut(s) 1407
BsiHKAI GWGCWC 2 cut(s) 163, 277
BsiSI CCGG 1 cut(s) 1227
BslI CCNNNNNNNGG 3 cut(s) 419, 1226, 1355
BsmAI GTCTC 5 cut(s) 464, 495, 723, 790, 1155
BsmBI CGTCTC 2 cut(s) 723, 1155
BsmI GAATGC 1 cut(s) 852
BsnI GGCC 5 cut(s) 71, 113, 394, 407, 968
Bsp1286I GDGCHC 2 cut(s) 163, 277
Bsp143I GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
Bsp1720I GCTNAGC 2 cut(s) 248, 276
Bsp19I CCATGG 2 cut(s) 1185, 1283
BspACI CCGC 2 cut(s) 574, 1317
BspANI GGCC 5 cut(s) 71, 113, 394, 407, 968
BspCNI CTCAG 6 cut(s) 118, 240, 289, 388, 632, 1129
BspLI GGNNCC 1 cut(s) 114
BspPI GGATC 3 cut(s) 743, 1001, 1316
BspTI CTTAAG 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 702, 1206
BsrI ACTGG 4 cut(s) 253, 456, 1090, 1150
BssECI CCNNGG 3 cut(s) 108, 1185, 1283
BssMI GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
BssNI GRCGYC 1 cut(s) 1325
BssT1I CCWWGG 3 cut(s) 108, 1185, 1283
Bst4CI ACNGT 4 cut(s) 167, 546, 776, 899
Bst6I CTCTTC 1 cut(s) 424
BstACI GRCGYC 1 cut(s) 1325
BstAFI CTTAAG 1 cut(s) 401
BstDEI CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1137
BstDSI CCRYGG 2 cut(s) 1185, 1283
BstENI CCTNNNNNAGG 1 cut(s) 1353
BstF5I GGATG 5 cut(s) 361, 658, 829, 1086, 1216
BstKTI GATC 9 cut(s) 594, 618, 625, 738, 870, 996, 1311, 1354, 1407
BstMAI GTCTC 5 cut(s) 464, 495, 723, 790, 1155
BstMBI GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
BstMCI CGRYCG 1 cut(s) 1407
BstMWI GCNNNNNNNGC 4 cut(s) 167, 327, 908, 1268
BstNSI RCATGY 4 cut(s) 885, 1106, 1240, 1293
BstSCI CCNGG 1 cut(s) 1226
BstSFI CTRYAG 4 cut(s) 667, 895, 1005, 1156
BstV1I GCAGC 6 cut(s) 449, 452, 930, 1177, 1291, 1332
BstV2I GAAGAC 2 cut(s) 777, 963
BstX2I RGATCY 2 cut(s) 615, 622
BstXI CCANNNNNNTGG 2 cut(s) 913, 1258
BstYI RGATCY 2 cut(s) 615, 622
BsuRI GGCC 5 cut(s) 71, 113, 394, 407, 968
BtgI CCRYGG 2 cut(s) 1185, 1283
BtgZI GCGATG 1 cut(s) 892
BtrI CACGTC 1 cut(s) 1165
BtsCI GGATG 5 cut(s) 361, 658, 829, 1086, 1216
BtsI GCAGTG 1 cut(s) 1052
BtsIMutI CAGTG 6 cut(s) 54, 172, 173, 726, 904, 1052
CaiI CAGNNNCTG 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 112, 406
CseI GACGC 1 cut(s) 1333
Csp6I GTAC 4 cut(s) 139, 451, 1293, 1431
CviQI GTAC 4 cut(s) 139, 451, 1293, 1431
DdeI CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1137
DpnI GATC 9 cut(s) 593, 617, 624, 737, 869, 995, 1310, 1353, 1406
DpnII GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
EaeI YGGCCR 1 cut(s) 966
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
Ecl136II GAGCTC 1 cut(s) 161
Eco130I CCWWGG 3 cut(s) 108, 1185, 1283
Eco24I GRGCYC 1 cut(s) 163
Eco32I GATATC 1 cut(s) 610
Eco53kI GAGCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 1353
EcoO109I RGGNCCY 2 cut(s) 112, 406
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 3 cut(s) 108, 1185, 1283
EcoT22I ATGCAT 1 cut(s) 887
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 3 cut(s) 108, 1185, 1283
Esp3I CGTCTC 2 cut(s) 723, 1155
FauI CCCGC 1 cut(s) 581
FbaI TGATCA 2 cut(s) 867, 1351
Fnu4HI GCNGC 6 cut(s) 438, 441, 919, 1191, 1280, 1346
FokI GGATG 5 cut(s) 368, 645, 836, 1093, 1203
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 6 cut(s) 438, 441, 919, 1191, 1280, 1346
FspBI CTAG 2 cut(s) 762, 863
GluI GCNGC 6 cut(s) 438, 441, 919, 1191, 1280, 1346
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 5 cut(s) 71, 113, 394, 407, 968
HapII CCGG 1 cut(s) 1227
HgaI GACGC 1 cut(s) 1333
Hin1I GRCGYC 1 cut(s) 1325
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 4 cut(s) 128, 746, 937, 980
HpaII CCGG 1 cut(s) 1227
HphI GGTGA 5 cut(s) 316, 539, 619, 803, 882
Hpy166II GTNNAC 2 cut(s) 306, 1431
Hpy188I TCNGA 5 cut(s) 127, 231, 622, 872, 950
Hpy188III TCNNGA 7 cut(s) 377, 613, 743, 941, 991, 997, 1136
Hpy8I GTNNAC 2 cut(s) 306, 1431
HpyAV CCTTC 4 cut(s) 419, 441, 1196, 1261
HpyCH4III ACNGT 4 cut(s) 167, 546, 776, 899
HpyCH4IV ACGT 1 cut(s) 1164
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 327, 908, 1268
HpyF3I CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1137
HpySE526I ACGT 1 cut(s) 1164
Hsp92I GRCGYC 1 cut(s) 1325
Ksp22I TGATCA 2 cut(s) 867, 1351
Kzo9I GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 6 cut(s) 449, 452, 930, 1177, 1291, 1332
LweI GCATC 2 cut(s) 346, 424
MaeI CTAG 2 cut(s) 762, 863
MaeII ACGT 1 cut(s) 1164
MaeIII GTNAC 8 cut(s) 493, 674, 776, 1098, 1112, 1180, 1246, 1321
MalI GATC 9 cut(s) 593, 617, 624, 737, 869, 995, 1310, 1353, 1406
MboI GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
MboII GAAGA 7 cut(s) 27, 338, 356, 441, 568, 782, 968
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 2 cut(s) 615, 622
MhlI GDGCHC 2 cut(s) 163, 277
MlsI TGGCCA 1 cut(s) 968
MluCI AATT 6 cut(s) 151, 513, 637, 711, 765, 1090
MluNI TGGCCA 1 cut(s) 968
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 9 cut(s) 77, 92, 182, 263, 332, 419, 641, 1007, 1010
Mox20I TGGCCA 1 cut(s) 968
Mph1103I ATGCAT 1 cut(s) 887
MscI TGGCCA 1 cut(s) 968
MseI TTAA 6 cut(s) 53, 74, 402, 810, 1152, 1422
MslI CAYNNNNRTG 4 cut(s) 354, 683, 1107, 1288
Msp20I TGGCCA 1 cut(s) 968
MspA1I CMGCKG 3 cut(s) 293, 443, 1004
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 1 cut(s) 1227
MspR9I CCNGG 1 cut(s) 1228
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 1 cut(s) 852
MwoI GCNNNNNNNGC 4 cut(s) 167, 327, 908, 1268
NciI CCSGG 1 cut(s) 1228
NcoI CCATGG 2 cut(s) 1185, 1283
NdeII GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
NlaIV GGNNCC 1 cut(s) 114
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 3 cut(s) 1098, 1246, 1321
NsiI ATGCAT 1 cut(s) 887
NspI RCATGY 4 cut(s) 885, 1106, 1240, 1293
PciI ACATGT 1 cut(s) 1289
PctI GAATGC 1 cut(s) 852
PfeI GAWTC 3 cut(s) 746, 937, 980
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 6 cut(s) 439, 442, 920, 1192, 1281, 1347
Ple19I CGATCG 1 cut(s) 1407
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PscI ACATGT 1 cut(s) 1289
Psp124BI GAGCTC 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 293
PspN4I GGNNCC 1 cut(s) 114
PspPI GGNCC 2 cut(s) 112, 406
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 2 cut(s) 615, 622
PvuI CGATCG 1 cut(s) 1407
PvuII CAGCTG 3 cut(s) 293, 443, 1004
RsaI GTAC 4 cut(s) 140, 452, 1294, 1432
RsaNI GTAC 4 cut(s) 139, 451, 1293, 1431
RseI CAYNNNNRTG 4 cut(s) 354, 683, 1107, 1288
SacI GAGCTC 1 cut(s) 163
SaqAI TTAA 6 cut(s) 53, 74, 402, 810, 1152, 1422
SatI GCNGC 6 cut(s) 438, 441, 919, 1191, 1280, 1346
Sau3AI GATC 9 cut(s) 591, 615, 622, 735, 867, 993, 1308, 1351, 1404
Sau96I GGNCC 2 cut(s) 112, 406
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 1228
SduI GDGCHC 2 cut(s) 163, 277
SfaNI GCATC 2 cut(s) 346, 424
SfcI CTRYAG 4 cut(s) 667, 895, 1005, 1156
SmiMI CAYNNNNRTG 4 cut(s) 354, 683, 1107, 1288
SmlI CTYRAG 3 cut(s) 33, 401, 601
SmoI CTYRAG 3 cut(s) 33, 401, 601
SpeI ACTAGT 1 cut(s) 862
Sse9I AATT 6 cut(s) 151, 513, 637, 711, 765, 1090
SsiI CCGC 2 cut(s) 574, 1317
SspMI CTAG 2 cut(s) 762, 863
SstI GAGCTC 1 cut(s) 163
StyD4I CCNGG 1 cut(s) 1226
StyI CCWWGG 3 cut(s) 108, 1185, 1283
TaaI ACNGT 4 cut(s) 167, 546, 776, 899
TaiI ACGT 1 cut(s) 1167
TaqI TCGA 1 cut(s) 1407
TaqII GACCGA 1 cut(s) 194
TasI AATT 6 cut(s) 151, 513, 637, 711, 765, 1090
TatI WGTACW 3 cut(s) 138, 1292, 1430
TfiI GAWTC 3 cut(s) 746, 937, 980
Tru1I TTAA 6 cut(s) 53, 74, 402, 810, 1152, 1422
Tru9I TTAA 6 cut(s) 53, 74, 402, 810, 1152, 1422
TscAI CASTG 6 cut(s) 54, 172, 180, 733, 904, 1059
TseFI GTSAC 3 cut(s) 1098, 1246, 1321
TseI GCWGC 6 cut(s) 437, 440, 918, 1190, 1279, 1345
Tsp45I GTSAC 3 cut(s) 1098, 1246, 1321
TspDTI ATGAA 5 cut(s) 41, 56, 164, 647, 1079
TspRI CASTG 6 cut(s) 54, 172, 180, 733, 904, 1059
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 401
XagI CCTNNNNNAGG 1 cut(s) 1353
XapI RAATTY 1 cut(s) 637
XceI RCATGY 4 cut(s) 885, 1106, 1240, 1293
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
XspI CTAG 2 cut(s) 762, 863
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.