Rroxscaffold_4G00299030

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005230
Physical Location & Seq
Reverse (-)
18933195 .. 18935748
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299030.1

Sequence Viewer

Length: 1461 bp
ATGGGATTGTTGAGAAGAAATCCCATGAAAACTCAAGTGATGAAATCAGTGTTAATAGTTGTTGTGGTGGCCTTAATACTAAGAGGGGGAGAGAGCAGAGTAGCCAAGGCCGAGCCTAAGGCCCCAATAAAATCTGAGTCCTTTGAGTACTCTGCTATGAATTGCAGAGCTCACAGTGCTTCACTGACCGAGTTTGGAGGAGTTGGAGATGGAGAGACATCAAATACAAAGGCTTTTCAGACTGCAATAAGTAAGCTGAGCCAGTTTGCAACAGATGGAGGTGCTCAGCTTTTTGTTCCAGCTGGGAAATGGTTGACAGGAAGCTTCAGCCTCACCAGCCATTTCACTCTTTATCTTCACAAGGATGCTGTTCTTCTTGCTTCTCAGGACTTGAATGAGTGGCCTGTGCTTAAGGCCCTTCCATCTTATGGCAGAGGAAGAGATGCAGCAGCTGGAAGGTACTCCAGTCTCATATTTGGAACAAATCTCACTGATGTCATTGTTACAGGTGACAATGGCACAATTGATGGTCAAGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTACCTGATTGAGATCATGTTCTCAAGTGATATCCAGATCTCAGATCTCACCCTCCTAAATTCTCCATCATGGAATGTTCATCCTGTCTATAGCAGTAACATTCTTGTGCAAGGGATTACTATCATTGCTCCAATTTCGTCTCCAAACACTGATGGGATCAATCCAGATTCTTGCACAAATACTAGAATTGAAGACTGTTACATAGTCTCTGGTGATGATTGTGTAGCTGTTAAGAGTGGTTGGGATGAGTATGGCATATCATTTGGAATGCCTACAAAACAACTAGTGATCAGACGGCTCACATGCATTTCACCCTACAGTGCAACCATCGCATTGGGCAGCGAAATGTCAGGTGGGATCCAAGATGTCCGAGCCGAAGACATCGTGGCCATCAATACCGAATCAGCCGTCAGGATCAAGACAGCTGTAGGGAGAGGAGGGTATGTGAAAGACATATATGTAAGGAGAATGACACTGCACACAATGAAATGGGCATTTTGGATGACTGGTAATTATGGGTCACATGCTGATGGTAACTATGACAAAAATGCCTTTCCTGAGATTACTGGGATTAACTATAGAGACGTGGTGGCTGAAAATGTAACCATGGCTGCAAGATTGGAAGGCATTGCCGACCATCCATTTACCGGGATTTGCATGTCCAATGTGACCATTGGATTGGCAAAGAAGGCAAAGAAGCAGCCATGGACATGTACTGATATTCAGGGGATCACAAGCGGTGTGACGCCAACGCCCTGTGGTCTGCTGCCTGATCAAGGAACTGCTAAACCTGGTGGTTGTGATTTTCCAGCAGATGATATACCGATCGACATGGTAGAGATTAAAAAGTGTACTTACAGGAGTACTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

486

Amino Acids

52.85

Weight (kDa)

7.06

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 155 - 438 5.7e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 428
AciI CCGC 2 cut(s) 583, 1326
AclWI GGATC 5 cut(s) 752, 940, 953, 1010, 1325
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 1 cut(s) 646
AcuI CTGAAG 1 cut(s) 310
AcyI GRCGYC 1 cut(s) 1334
AfaI GTAC 5 cut(s) 149, 461, 1303, 1441, 1453
AfiI CCNNNNNNNGG 3 cut(s) 428, 1235, 1364
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 1298
AgsI TTSAA 2 cut(s) 394, 779
AhlI ACTAGT 1 cut(s) 871
AjiI CACGTC 1 cut(s) 1174
AjnI CCWGG 1 cut(s) 1378
AluBI AGCT 9 cut(s) 170, 256, 289, 302, 324, 452, 571, 815, 1013
AluI AGCT 9 cut(s) 170, 256, 289, 302, 324, 452, 571, 815, 1013
Alw21I GWGCWC 2 cut(s) 172, 286
Alw26I GTCTC 5 cut(s) 209, 473, 732, 799, 1164
AlwI GGATC 5 cut(s) 752, 940, 953, 1010, 1325
AlwNI CAGNNNCTG 1 cut(s) 452
AoxI GGCC 6 cut(s) 69, 108, 120, 401, 414, 975
ApeKI GCWGC 6 cut(s) 446, 449, 927, 1199, 1288, 1354
ApoI RAATTY 1 cut(s) 646
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
AspS9I GGNCC 2 cut(s) 121, 415
AsuC2I CCSGG 1 cut(s) 1237
AsuHPI GGTGA 6 cut(s) 325, 521, 548, 628, 812, 891
AxyI CCTNAGG 1 cut(s) 117
BaeI ACNNNNGTAYC 2 cut(s) 451, 484
BalI TGGCCA 1 cut(s) 977
BamHI GGATCC 1 cut(s) 945
BanII GRGCYC 1 cut(s) 172
BarI GAAGNNNNNNTAC 2 cut(s) 560, 592
BbsI GAAGAC 2 cut(s) 786, 972
Bbv12I GWGCWC 2 cut(s) 172, 286
BbvI GCAGC 6 cut(s) 458, 461, 939, 1186, 1300, 1341
BceAI ACGGC 2 cut(s) 899, 980
BciT130I CCWGG 1 cut(s) 1380
BclI TGATCA 2 cut(s) 876, 1360
BcnI CCSGG 1 cut(s) 1237
BcoDI GTCTC 5 cut(s) 209, 473, 732, 799, 1164
BcuI ACTAGT 1 cut(s) 871
BfaI CTAG 2 cut(s) 771, 872
BfmI CTRYAG 4 cut(s) 676, 904, 1014, 1165
BfrI CTTAAG 1 cut(s) 410
BglII AGATCT 2 cut(s) 624, 631
BisI GCNGC 6 cut(s) 447, 450, 928, 1200, 1289, 1355
BlpI GCTNAGC 2 cut(s) 257, 285
BlsI GCNGC 6 cut(s) 448, 451, 929, 1201, 1290, 1356
BmcAI AGTACT 2 cut(s) 149, 1453
Bme1390I CCNGG 2 cut(s) 1237, 1380
BmgBI CACGTC 1 cut(s) 1174
BmgT120I GGNCC 2 cut(s) 121, 415
BmiI GGNNCC 2 cut(s) 123, 947
BmrFI CCNGG 2 cut(s) 1237, 1380
BmrI ACTGGG 1 cut(s) 1164
BmsI GCATC 2 cut(s) 355, 433
BmuI ACTGGG 1 cut(s) 1164
BpiI GAAGAC 2 cut(s) 786, 972
BpmI CTGGAG 1 cut(s) 448
Bpu1102I GCTNAGC 2 cut(s) 257, 285
BpuEI CTTGAG 2 cut(s) 18, 595
BpuMI CCSGG 1 cut(s) 1237
BsaBI GATNNNNATC 2 cut(s) 599, 707
BsaHI GRCGYC 1 cut(s) 1334
BsaJI CCNNGG 3 cut(s) 105, 1194, 1292
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 3 cut(s) 428, 1235, 1364
Bse1I ACTGG 4 cut(s) 262, 465, 1099, 1159
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 2 cut(s) 711, 1215
Bse8I GATNNNNATC 2 cut(s) 599, 707
BseBI CCWGG 1 cut(s) 1380
BseDI CCNNGG 3 cut(s) 105, 1194, 1292
BseGI GGATG 5 cut(s) 370, 667, 838, 1095, 1225
BseJI GATNNNNATC 2 cut(s) 599, 707
BseLI CCNNNNNNNGG 3 cut(s) 428, 1235, 1364
BseMI GCAATG 2 cut(s) 711, 1215
BseMII CTCAG 6 cut(s) 126, 248, 299, 398, 642, 1137
BseNI ACTGG 4 cut(s) 262, 465, 1099, 1159
BseRI GAGGAG 2 cut(s) 213, 1038
BseXI GCAGC 6 cut(s) 458, 461, 939, 1186, 1300, 1341
BseYI CCCAGC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 1049
Bsh1285I CGRYCG 1 cut(s) 1416
BshFI GGCC 6 cut(s) 71, 110, 122, 403, 416, 977
BsiEI CGRYCG 1 cut(s) 1416
BsiHKAI GWGCWC 2 cut(s) 172, 286
BsiSI CCGG 1 cut(s) 1236
BslI CCNNNNNNNGG 3 cut(s) 428, 1235, 1364
BsmAI GTCTC 5 cut(s) 209, 473, 732, 799, 1164
BsmBI CGTCTC 2 cut(s) 732, 1164
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 6 cut(s) 71, 110, 122, 403, 416, 977
Bsp1286I GDGCHC 2 cut(s) 172, 286
Bsp1720I GCTNAGC 2 cut(s) 257, 285
Bsp19I CCATGG 2 cut(s) 1194, 1292
BspACI CCGC 2 cut(s) 583, 1326
BspANI GGCC 6 cut(s) 71, 110, 122, 403, 416, 977
BspCNI CTCAG 6 cut(s) 127, 249, 298, 397, 641, 1138
BspLI GGNNCC 2 cut(s) 123, 947
BspPI GGATC 5 cut(s) 752, 940, 953, 1010, 1325
BspTI CTTAAG 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 711, 1215
BsrI ACTGG 4 cut(s) 262, 465, 1099, 1159
BssECI CCNNGG 3 cut(s) 105, 1194, 1292
BssNI GRCGYC 1 cut(s) 1334
BssT1I CCWWGG 3 cut(s) 105, 1194, 1292
Bst2UI CCWGG 1 cut(s) 1380
Bst4CI ACNGT 5 cut(s) 176, 555, 785, 908, 1456
Bst6I CTCTTC 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 1334
BstAFI CTTAAG 1 cut(s) 410
BstDEI CTNAG 8 cut(s) 80, 117, 135, 257, 285, 384, 628, 1146
BstDSI CCRYGG 2 cut(s) 1194, 1292
BstENI CCTNNNNNAGG 1 cut(s) 1362
BstF5I GGATG 5 cut(s) 370, 667, 838, 1095, 1225
BstMAI GTCTC 5 cut(s) 209, 473, 732, 799, 1164
BstMCI CGRYCG 1 cut(s) 1416
BstMWI GCNNNNNNNGC 4 cut(s) 176, 336, 917, 1277
BstNI CCWGG 1 cut(s) 1380
BstNSI RCATGY 4 cut(s) 894, 1115, 1249, 1302
BstSCI CCNGG 2 cut(s) 1235, 1378
BstSFI CTRYAG 4 cut(s) 676, 904, 1014, 1165
BstV1I GCAGC 6 cut(s) 458, 461, 939, 1186, 1300, 1341
BstV2I GAAGAC 2 cut(s) 786, 972
BstX2I RGATCY 3 cut(s) 624, 631, 945
BstXI CCANNNNNNTGG 2 cut(s) 922, 1267
BstYI RGATCY 3 cut(s) 624, 631, 945
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 6 cut(s) 71, 110, 122, 403, 416, 977
BtgI CCRYGG 2 cut(s) 1194, 1292
BtgZI GCGATG 1 cut(s) 901
BtrI CACGTC 1 cut(s) 1174
BtsCI GGATG 5 cut(s) 370, 667, 838, 1095, 1225
BtsI GCAGTG 1 cut(s) 1061
BtsIMutI CAGTG 7 cut(s) 54, 181, 182, 489, 735, 913, 1061
CaiI CAGNNNCTG 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 121, 415
CseI GACGC 1 cut(s) 1342
CsiI ACCWGGT 1 cut(s) 1378
Csp6I GTAC 5 cut(s) 148, 460, 1302, 1440, 1452
CviQI GTAC 5 cut(s) 148, 460, 1302, 1440, 1452
DdeI CTNAG 8 cut(s) 80, 117, 135, 257, 285, 384, 628, 1146
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 433
Ecl136II GAGCTC 1 cut(s) 170
Eco130I CCWWGG 3 cut(s) 105, 1194, 1292
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 619
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 310
Eco81I CCTNAGG 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 1362
EcoO109I RGGNCCY 2 cut(s) 121, 415
EcoRII CCWGG 1 cut(s) 1378
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 3 cut(s) 105, 1194, 1292
EcoT22I ATGCAT 1 cut(s) 896
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 3 cut(s) 105, 1194, 1292
Esp3I CGTCTC 2 cut(s) 732, 1164
FauI CCCGC 1 cut(s) 590
FbaI TGATCA 2 cut(s) 876, 1360
Fnu4HI GCNGC 6 cut(s) 447, 450, 928, 1200, 1289, 1355
FokI GGATG 5 cut(s) 377, 654, 845, 1102, 1212
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 6 cut(s) 447, 450, 928, 1200, 1289, 1355
FspBI CTAG 2 cut(s) 771, 872
GluI GCNGC 6 cut(s) 447, 450, 928, 1200, 1289, 1355
GsaI CCCAGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 448
HaeIII GGCC 6 cut(s) 71, 110, 122, 403, 416, 977
HapII CCGG 1 cut(s) 1236
HgaI GACGC 1 cut(s) 1342
Hin1I GRCGYC 1 cut(s) 1334
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 3 cut(s) 137, 755, 989
HpaII CCGG 1 cut(s) 1236
HphI GGTGA 6 cut(s) 325, 521, 548, 628, 812, 891
Hpy166II GTNNAC 2 cut(s) 315, 1440
Hpy188I TCNGA 5 cut(s) 136, 240, 631, 881, 959
Hpy188III TCNNGA 6 cut(s) 386, 622, 752, 1000, 1006, 1145
Hpy8I GTNNAC 2 cut(s) 315, 1440
HpyAV CCTTC 4 cut(s) 428, 450, 1205, 1270
HpyCH4III ACNGT 5 cut(s) 176, 555, 785, 908, 1456
HpyCH4IV ACGT 1 cut(s) 1173
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 336, 917, 1277
HpyF3I CTNAG 8 cut(s) 80, 117, 135, 257, 285, 384, 628, 1146
HpySE526I ACGT 1 cut(s) 1173
Hsp92I GRCGYC 1 cut(s) 1334
Ksp22I TGATCA 2 cut(s) 876, 1360
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 6 cut(s) 458, 461, 939, 1186, 1300, 1341
LweI GCATC 2 cut(s) 355, 433
MabI ACCWGGT 1 cut(s) 1378
MaeI CTAG 2 cut(s) 771, 872
MaeII ACGT 1 cut(s) 1173
MaeIII GTNAC 9 cut(s) 502, 509, 683, 785, 1107, 1121, 1189, 1255, 1330
MboII GAAGA 7 cut(s) 27, 347, 365, 450, 577, 791, 977
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 3 cut(s) 624, 631, 945
MhlI GDGCHC 2 cut(s) 172, 286
MlsI TGGCCA 1 cut(s) 977
MluCI AATT 6 cut(s) 160, 522, 646, 720, 774, 1099
MluNI TGGCCA 1 cut(s) 977
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 8 cut(s) 77, 191, 272, 341, 428, 650, 1016, 1019
Mox20I TGGCCA 1 cut(s) 977
Mph1103I ATGCAT 1 cut(s) 896
MscI TGGCCA 1 cut(s) 977
MseI TTAA 6 cut(s) 53, 74, 411, 819, 1161, 1431
MslI CAYNNNNRTG 4 cut(s) 363, 692, 1116, 1297
Msp20I TGGCCA 1 cut(s) 977
MspA1I CMGCKG 3 cut(s) 302, 452, 1013
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 1 cut(s) 1236
MspR9I CCNGG 2 cut(s) 1237, 1380
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 1 cut(s) 1380
MwoI GCNNNNNNNGC 4 cut(s) 176, 336, 917, 1277
NciI CCSGG 1 cut(s) 1237
NcoI CCATGG 2 cut(s) 1194, 1292
NlaIV GGNNCC 2 cut(s) 123, 947
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 4 cut(s) 509, 1107, 1255, 1330
NsiI ATGCAT 1 cut(s) 896
NspI RCATGY 4 cut(s) 894, 1115, 1249, 1302
PciI ACATGT 1 cut(s) 1298
PctI GAATGC 1 cut(s) 861
PfeI GAWTC 2 cut(s) 755, 989
PflMI CCANNNNNTGG 1 cut(s) 428
PkrI GCNGC 6 cut(s) 448, 451, 929, 1201, 1290, 1356
Ple19I CGATCG 1 cut(s) 1416
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PscI ACATGT 1 cut(s) 1298
Psp124BI GAGCTC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 1378
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 1 cut(s) 1378
PspN4I GGNNCC 2 cut(s) 123, 947
PspPI GGNCC 2 cut(s) 121, 415
PstNI CAGNNNCTG 1 cut(s) 452
PsuI RGATCY 3 cut(s) 624, 631, 945
PvuI CGATCG 1 cut(s) 1416
PvuII CAGCTG 3 cut(s) 302, 452, 1013
RsaI GTAC 5 cut(s) 149, 461, 1303, 1441, 1453
RsaNI GTAC 5 cut(s) 148, 460, 1302, 1440, 1452
RseI CAYNNNNRTG 4 cut(s) 363, 692, 1116, 1297
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 6 cut(s) 53, 74, 411, 819, 1161, 1431
SatI GCNGC 6 cut(s) 447, 450, 928, 1200, 1289, 1355
Sau96I GGNCC 2 cut(s) 121, 415
ScaI AGTACT 2 cut(s) 149, 1453
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 1237, 1380
SduI GDGCHC 2 cut(s) 172, 286
SexAI ACCWGGT 1 cut(s) 1378
SfaNI GCATC 2 cut(s) 355, 433
SfcI CTRYAG 4 cut(s) 676, 904, 1014, 1165
SmiMI CAYNNNNRTG 4 cut(s) 363, 692, 1116, 1297
SmlI CTYRAG 3 cut(s) 33, 410, 610
SmoI CTYRAG 3 cut(s) 33, 410, 610
SpeI ACTAGT 1 cut(s) 871
Sse9I AATT 6 cut(s) 160, 522, 646, 720, 774, 1099
SsiI CCGC 2 cut(s) 583, 1326
SspMI CTAG 2 cut(s) 771, 872
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 1235, 1378
StyI CCWWGG 3 cut(s) 105, 1194, 1292
TaaI ACNGT 5 cut(s) 176, 555, 785, 908, 1456
TaiI ACGT 1 cut(s) 1176
TaqI TCGA 1 cut(s) 1416
TaqII GACCGA 1 cut(s) 203
TasI AATT 6 cut(s) 160, 522, 646, 720, 774, 1099
TatI WGTACW 4 cut(s) 147, 1301, 1439, 1451
TfiI GAWTC 2 cut(s) 755, 989
Tru1I TTAA 6 cut(s) 53, 74, 411, 819, 1161, 1431
Tru9I TTAA 6 cut(s) 53, 74, 411, 819, 1161, 1431
TscAI CASTG 7 cut(s) 54, 181, 189, 496, 742, 913, 1068
TseFI GTSAC 4 cut(s) 509, 1107, 1255, 1330
TseI GCWGC 6 cut(s) 446, 449, 927, 1199, 1288, 1354
Tsp45I GTSAC 4 cut(s) 509, 1107, 1255, 1330
TspDTI ATGAA 5 cut(s) 41, 56, 173, 656, 1088
TspRI CASTG 7 cut(s) 54, 181, 189, 496, 742, 913, 1068
Van91I CCANNNNNTGG 1 cut(s) 428
Vha464I CTTAAG 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 1362
XapI RAATTY 1 cut(s) 646
XceI RCATGY 4 cut(s) 894, 1115, 1249, 1302
XcmI CCANNNNNNNNNTGG 1 cut(s) 306
XspI CTAG 2 cut(s) 771, 872
ZrmI AGTACT 2 cut(s) 149, 1453
Zsp2I ATGCAT 1 cut(s) 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.