Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0005230

Physical Location & Seq

Genomic Coordinates Reverse (-)

15180674 .. 15182548

Gene Structure 1875 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG148200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGAAAACCCTTCAGGTGATGAGATCACCACTGTTAATACATGTTGTGGTCTGCTTACTACTTATTGGAGGGGGTGACAGCAGAAAAGTATTCAAGCTCTCCGAGGAGAAATTCGAATCCGAATCCTCGTTCGAGTACAATGCCATCAGCTGCAGAGCCCACAGCGCTTCGCTCACTGATTTCGGAGGAGTAGGAGACGGCAAGACATCAAACACAAAGGCCTTTCAAGCCGCAATCAACCACCTGAGTCAGTACTCAACCCAAGGAGGTGCTCAGCTTTACGTTCCGGCCGGGAAGTACTTGACCGGTAGCTTCAACCTCACCAGCCACTTCACTCTTTACCTTCACAAAGAGGCTGTTCTCCTCGCTTCTCAAGACCCCCAAGAATGGCCGGTGCTCAAACCACTCCCGTCTTACGGCAGAGGAAGGGACGCAGCAGGGGGACGCTACACCAGCCTCATTTTCGGAACCAACCTCACCGACGTCATCATCACCGGAGACAACGGCACAATCGACGGTCAAGGTGAATTCTGGTGGAACAAATTCCACAGTAAAAAGTTACAGTACACCCGCCCGTACCTGATAGAGATCATGTTCTCCAGCAACGTGCAAATATCTCATCTCACTCTTTTGAACTCTCCATCATGGAATGTCCATCCCATTTACAGCAGCAATATACTAGTGCAAGGATTGACCATCATAGCTCCGGTGAGGTCTCCGAACACAGATGGAATTAATCCGGATTCATGCACAAACGTCAAGATTGAGGACTGTTACATTGTCTCCGGCGACGACTGCGTGGCCGTCAAGAGCGGTTGGGACCAGTACGGCGTGTCGTTTGGTATGCCAACCAAGCAACTAGTGATCAGACGACTTACTTGTGTTTCGCCTTACAGCGCCGCCATTGCATTGGGCAGCGAGATGTCCGGTGGGATTGAAGACGTCAGAGCAGAAGACATCGTGGCCGTTGATACGGAATCCGGGGTGAGGATCAAGACAGCTGTAGGGCGAGGAGGGTACGTGAGGGACGTGTACTTAAGGAGAATGGAAATGCACACCATGAAGTGGGCGTTTTGGATGAGTGGCGATTATGGGTCACACGCTGACGATGGCTATGACAAGAAGGCGTTTCCTGAGATCAAAGGGATTCACTACAGGGATGTGGTGGCTGATAATGTGACAATGGCAGCGAAACTGGAGGGGATTGCTGGCCATCCGTTTACGGGGATTTGCATGTCGAATGTGACAGTTGGATTGGGTGCTAAAGCAAAGAAGCAGCCGTGGAGCTGTGCTGATATTGAGGGGGTTGCAAGTGGCGTGACGCCGACACCGTGTGATTTGCTACATGGTGGTGATCCGAGTCAGGGTGATGTTGCTAGCGGTGGCTGTGATTTTCCTGTGGATGTTGTACCAATTGACACGGTGGAGATGAAAAGATGTAGGTATGATAGTAGGATGAAGTCGCCTCCTTCATCTCCTAGTCCTTCTAGGAGTCCAAGTTCGTCTGATCAGGACCCTGCGGACAAGGATCCAGCTCCCAGTCCATCATCATTAGGAGGGCACGAGCTTGAGCACTACTATTCGACACCGCCAAAACCAGCTGATAGCGTGACGTCAGAATCACCATCAACAAGTTCTGCTGGGCCTGATGCACCTTATTATGACATTCATGGTCGACGATCATCATCAAAAGGAAAGCCCAAAAAATGGAAGCCGAAGCGGTCACCACCACCTCCTCCTTCTCCTGCTGCGCCTGCGCCATTGCCTCCTAAGAATAATGCAAGCGCCGCTGCTGAGATGGGTACTGTTTGGACTCGGTTTGGCTTCGCTTTTGGTTGCGTTCTTCTGGCCATCATGAACAATCATATGTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

624

Amino Acids

67.38

Weight (kDa)

6.78

Isoelectric Point (pI)

58.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	58 - 103	7.5e-09	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	149 - 434	6.2e-39	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005230)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61490 AT3G61490 AT3G61490 AT3G61490 AT3G61490
fragaria_vesca	FvH4_3g12401 FvH4_7g15040
malus_domestica	MD00G1112000.v1.1 MD01G1069000.v1.1
prunus_persica	Prupe.2G175100_v2.0.a1 Prupe.2G175100_v2.0.a1
pyrus_communis	pycom01g09850
rosa_chinensis	RchiOBHm_Chr1g0357161 RchiOBHm_Chr5g0020281
rosa_laevigata	RLG00000028048
rosa_multiflora	Rmu_sc0005967.1_g000009 Rmu_sc0006896.1_g000016 Rmu_sc0023664.1_g000001
rosa_roxburghii	Rroxscaffold_4G00299030
rosa_rugosa	Rorug01G0254400 Rorug05G0058300
rosa_samantha	Rh1AG268700 Rh1BG236400 Rh1DG263500 Rh5BG148200 Rh5CG159700 Rh5DG147100
rosa_wichuraiana	Rw1G023710 Rw5G013160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	486, 945, 1616
AccB7I	CCANNNNNTGG	2 cut(s)	1065, 1707
AccBSI	CCGCTC	1 cut(s)	813
AccI	GTMKAC	1 cut(s)	1675
AccIII	TCCGGA	1 cut(s)	739
AciI	CCGC	9 cut(s)	231, 571, 813, 900, 1380, 1520, 1589, 1720, 1788
AclWI	GGATC	4 cut(s)	998, 1349, 1523, 1536
AcoI	YGGCCR	6 cut(s)	288, 389, 801, 963, 1210, 1848
AcsI	RAATTY	3 cut(s)	110, 527, 542
AcyI	GRCGYC	4 cut(s)	483, 942, 1322, 1613
AdeI	CACNNNGTG	1 cut(s)	1334
AfeI	AGCGCT	1 cut(s)	166
AfiI	CCNNNNNNNGG	7 cut(s)	387, 416, 987, 1065, 1223, 1364, 1707
AflII	CTTAAG	1 cut(s)	1036
AflIII	ACRYGT	2 cut(s)	40, 1029
AgeI	ACCGGT	1 cut(s)	305
AgsI	TTSAA	5 cut(s)	94, 227, 316, 634, 938
AhlI	ACTAGT	2 cut(s)	679, 859
AjiI	CACGTC	1 cut(s)	1030
AloI	GAACNNNNNNTCC	2 cut(s)	1483, 1515
Alw21I	GWGCWC	3 cut(s)	274, 399, 1575
Alw26I	GTCTC	4 cut(s)	189, 492, 720, 787
AlwI	GGATC	4 cut(s)	998, 1349, 1523, 1536
Aor13HI	TCCGGA	1 cut(s)	739
Aor51HI	AGCGCT	1 cut(s)	166
AoxI	GGCC	8 cut(s)	219, 288, 389, 801, 963, 1210, 1643, 1848
ApeKI	GCWGC	8 cut(s)	150, 434, 669, 915, 1187, 1276, 1748, 1790
ApoI	RAATTY	3 cut(s)	110, 527, 542
AseI	ATTAAT	1 cut(s)	735
AsiGI	ACCGGT	1 cut(s)	305
Asp700I	GAANNNNTTC	1 cut(s)	542
AspLEI	GCGC	5 cut(s)	167, 899, 1753, 1759, 1787
AspS9I	GGNCC	3 cut(s)	820, 1513, 1643
AsuC2I	CCSGG	2 cut(s)	292, 982
AsuII	TTCGAA	1 cut(s)	114
AsuNHI	GCTAGC	1 cut(s)	1376
AvaII	GGWCC	2 cut(s)	820, 1513
BaeGI	GKGCMC	1 cut(s)	1563
BalI	TGGCCA	2 cut(s)	1212, 1850
BamHI	GGATCC	1 cut(s)	1528
BanII	GRGCYC	1 cut(s)	160
BauI	CACGAG	1 cut(s)	1562
BbsI	GAAGAC	2 cut(s)	945, 960
Bbv12I	GWGCWC	3 cut(s)	274, 399, 1575
BbvI	GCAGC	8 cut(s)	137, 446, 681, 927, 1199, 1288, 1735, 1777
BceAI	ACGGC	7 cut(s)	214, 433, 520, 788, 844, 950, 1264
BcgI	CGANNNNNNTGC	2 cut(s)	122, 156
BclI	TGATCA	2 cut(s)	864, 1507
BcnI	CCSGG	2 cut(s)	292, 982
BcoDI	GTCTC	4 cut(s)	189, 492, 720, 787
BcuI	ACTAGT	2 cut(s)	679, 859
BfaI	CTAG	5 cut(s)	680, 860, 1377, 1479, 1488
BfmI	CTRYAG	3 cut(s)	151, 1002, 1153
BfoI	RGCGCY	3 cut(s)	168, 900, 1788
BfrI	CTTAAG	1 cut(s)	1036
BlpI	GCTNAGC	1 cut(s)	273
BmcAI	AGTACT	2 cut(s)	254, 299
Bme1390I	CCNGG	2 cut(s)	292, 982
Bme18I	GGWCC	2 cut(s)	820, 1513
BmgBI	CACGTC	1 cut(s)	1030
BmgT120I	GGNCC	3 cut(s)	820, 1513, 1643
BmiI	GGNNCC	4 cut(s)	469, 821, 1515, 1530
BmrFI	CCNGG	2 cut(s)	292, 982
BmrI	ACTGGG	1 cut(s)	1533
BmsI	GCATC	1 cut(s)	1639
BmtI	GCTAGC	1 cut(s)	1380
BmuI	ACTGGG	1 cut(s)	1533
BpiI	GAAGAC	2 cut(s)	945, 960
BpmI	CTGGAG	2 cut(s)	583, 1217
Bpu1102I	GCTNAGC	1 cut(s)	273
Bpu14I	TTCGAA	1 cut(s)	114
BpuEI	CTTGAG	2 cut(s)	357, 1589
BpuMI	CCSGG	2 cut(s)	292, 982
BsaAI	YACGTR	1 cut(s)	1021
BsaBI	GATNNNNATC	2 cut(s)	587, 1684
BsaHI	GRCGYC	4 cut(s)	483, 942, 1322, 1613
BsaI	GGTCTC	1 cut(s)	720
BsaJI	CCNNGG	4 cut(s)	102, 262, 981, 1280
BsaWI	WCCGGW	5 cut(s)	305, 494, 706, 739, 926
BsaXI	ACNNNNNCTCC	6 cut(s)	767, 797, 1005, 1035, 1483, 1513
Bsc4I	CCNNNNNNNGG	7 cut(s)	387, 416, 987, 1065, 1223, 1364, 1707
Bse118I	RCCGGY	2 cut(s)	305, 391
Bse1I	ACTGG	3 cut(s)	823, 1200, 1539
Bse3DI	GCAATG	2 cut(s)	903, 1760
Bse8I	GATNNNNATC	2 cut(s)	587, 1684
BseAI	TCCGGA	1 cut(s)	739
BseDI	CCNNGG	4 cut(s)	102, 262, 981, 1280
BseGI	GGATG	6 cut(s)	655, 1083, 1165, 1213, 1408, 1461
BseJI	GATNNNNATC	2 cut(s)	587, 1684
BseLI	CCNNNNNNNGG	7 cut(s)	387, 416, 987, 1065, 1223, 1364, 1707
BseMI	GCAATG	2 cut(s)	903, 1760
BseMII	CTCAG	4 cut(s)	236, 287, 1125, 1785
BseNI	ACTGG	3 cut(s)	823, 1200, 1539
BseRI	GAGGAG	5 cut(s)	119, 201, 353, 1026, 1725
BseSI	GKGCMC	1 cut(s)	1563
BseX3I	CGGCCG	1 cut(s)	288
BseXI	GCAGC	8 cut(s)	137, 446, 681, 927, 1199, 1288, 1735, 1777
BseYI	CCCAGC	1 cut(s)	1640
Bsh1285I	CGRYCG	1 cut(s)	291
BshFI	GGCC	8 cut(s)	221, 290, 391, 803, 965, 1212, 1645, 1850
BshTI	ACCGGT	1 cut(s)	305
BsiEI	CGRYCG	1 cut(s)	291
BsiHKAI	GWGCWC	3 cut(s)	274, 399, 1575
BslFI	GGGAC	4 cut(s)	443, 456, 833, 1040
BslI	CCNNNNNNNGG	7 cut(s)	387, 416, 987, 1065, 1223, 1364, 1707
BsmAI	GTCTC	4 cut(s)	189, 492, 720, 787
BsmBI	CGTCTC	1 cut(s)	189
BsmFI	GGGAC	4 cut(s)	443, 456, 833, 1040
BsnI	GGCC	8 cut(s)	221, 290, 391, 803, 965, 1212, 1645, 1850
Bso31I	GGTCTC	1 cut(s)	720
Bsp119I	TTCGAA	1 cut(s)	114
Bsp1286I	GDGCHC	5 cut(s)	160, 274, 399, 1563, 1575
Bsp13I	TCCGGA	1 cut(s)	739
Bsp143I	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
Bsp1720I	GCTNAGC	1 cut(s)	273
BspACI	CCGC	9 cut(s)	231, 571, 813, 900, 1380, 1520, 1589, 1720, 1788
BspANI	GGCC	8 cut(s)	221, 290, 391, 803, 965, 1212, 1645, 1850
BspCNI	CTCAG	4 cut(s)	237, 286, 1126, 1786
BspEI	TCCGGA	1 cut(s)	739
BspHI	TCATGA	1 cut(s)	1854
BspLI	GGNNCC	4 cut(s)	469, 821, 1515, 1530
BspMAI	CTGCAG	1 cut(s)	155
BspOI	GCTAGC	1 cut(s)	1380
BspPI	GGATC	4 cut(s)	998, 1349, 1523, 1536
BspT104I	TTCGAA	1 cut(s)	114
BspTI	CTTAAG	1 cut(s)	1036
BspTNI	GGTCTC	1 cut(s)	720
BsrBI	CCGCTC	1 cut(s)	813
BsrDI	GCAATG	2 cut(s)	903, 1760
BsrFI	RCCGGY	2 cut(s)	305, 391
BsrI	ACTGG	3 cut(s)	823, 1200, 1539
BssAI	RCCGGY	2 cut(s)	305, 391
BssECI	CCNNGG	4 cut(s)	102, 262, 981, 1280
BssMI	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
BssNI	GRCGYC	4 cut(s)	483, 942, 1322, 1613
BssSI	CACGAG	1 cut(s)	1562
BssT1I	CCWWGG	1 cut(s)	262
Bst2BI	CACGAG	1 cut(s)	1562
Bst4CI	ACNGT	9 cut(s)	33, 518, 551, 564, 773, 1249, 1332, 1423, 1807
BstACI	GRCGYC	4 cut(s)	483, 942, 1322, 1613
BstAFI	CTTAAG	1 cut(s)	1036
BstBAI	YACGTR	1 cut(s)	1021
BstBI	TTCGAA	1 cut(s)	114
BstC8I	GCNNGC	4 cut(s)	1210, 1378, 1755, 1783
BstDEI	CTNAG	5 cut(s)	245, 273, 1134, 1770, 1794
BstDSI	CCRYGG	1 cut(s)	1280
BstEII	GGTNACC	1 cut(s)	1722
BstF5I	GGATG	6 cut(s)	655, 1083, 1165, 1213, 1408, 1461
BstH2I	RGCGCY	3 cut(s)	168, 900, 1788
BstHHI	GCGC	5 cut(s)	167, 899, 1753, 1759, 1787
BstKTI	GATC	9 cut(s)	26, 591, 867, 993, 1140, 1357, 1510, 1531, 1682
BstMAI	GTCTC	4 cut(s)	189, 492, 720, 787
BstMBI	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
BstMCI	CGRYCG	1 cut(s)	291
BstMWI	GCNNNNNNNGC	8 cut(s)	164, 227, 453, 795, 853, 905, 1754, 1787
BstNSI	RCATGY	2 cut(s)	44, 1237
BstPI	GGTNACC	1 cut(s)	1722
BstSCI	CCNGG	2 cut(s)	290, 980
BstSFI	CTRYAG	3 cut(s)	151, 1002, 1153
BstSLI	GKGCMC	1 cut(s)	1563
BstV1I	GCAGC	8 cut(s)	137, 446, 681, 927, 1199, 1288, 1735, 1777
BstV2I	GAAGAC	2 cut(s)	945, 960
BstX2I	RGATCY	1 cut(s)	1528
BstXI	CCANNNNNNTGG	1 cut(s)	910
BstYI	RGATCY	1 cut(s)	1528
BstZI	CGGCCG	1 cut(s)	288
BsuRI	GGCC	8 cut(s)	221, 290, 391, 803, 965, 1212, 1645, 1850
BtgI	CCRYGG	1 cut(s)	1280
BtrI	CACGTC	1 cut(s)	1030
BtsCI	GGATG	6 cut(s)	655, 1083, 1165, 1213, 1408, 1461
BtsIMutI	CAGTG	2 cut(s)	29, 174
Cac8I	GCNNGC	4 cut(s)	1210, 1378, 1755, 1783
CciI	TCATGA	1 cut(s)	1854
CfoI	GCGC	5 cut(s)	167, 899, 1753, 1759, 1787
Cfr10I	RCCGGY	2 cut(s)	305, 391
Cfr13I	GGNCC	3 cut(s)	820, 1513, 1643
CseI	GACGC	3 cut(s)	440, 453, 1330
CspAI	ACCGGT	1 cut(s)	305
CviAII	CATG	9 cut(s)	41, 592, 645, 747, 1060, 1234, 1346, 1670, 1855
DdeI	CTNAG	5 cut(s)	245, 273, 1134, 1770, 1794
DpnI	GATC	9 cut(s)	25, 590, 866, 992, 1139, 1356, 1509, 1530, 1681
DpnII	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
DraIII	CACNNNGTG	1 cut(s)	1334
EaeI	YGGCCR	6 cut(s)	288, 389, 801, 963, 1210, 1848
EagI	CGGCCG	1 cut(s)	288
EclXI	CGGCCG	1 cut(s)	288
Eco130I	CCWWGG	1 cut(s)	262
Eco147I	AGGCCT	1 cut(s)	221
Eco24I	GRGCYC	1 cut(s)	160
Eco31I	GGTCTC	1 cut(s)	720
Eco47I	GGWCC	2 cut(s)	820, 1513
Eco47III	AGCGCT	1 cut(s)	166
Eco52I	CGGCCG	1 cut(s)	288
Eco91I	GGTNACC	1 cut(s)	1722
EcoO109I	RGGNCCY	1 cut(s)	1513
EcoO65I	GGTNACC	1 cut(s)	1722
EcoRI	GAATTC	1 cut(s)	527
EcoT14I	CCWWGG	1 cut(s)	262
EcoT38I	GRGCYC	1 cut(s)	160
ErhI	CCWWGG	1 cut(s)	262
Esp3I	CGTCTC	1 cut(s)	189
FaeI	CATG	9 cut(s)	44, 595, 648, 750, 1063, 1237, 1349, 1673, 1858
FaqI	GGGAC	4 cut(s)	443, 456, 833, 1040
FatI	CATG	9 cut(s)	40, 591, 644, 746, 1059, 1233, 1345, 1669, 1854
FauI	CCCGC	1 cut(s)	578
FauNDI	CATATG	1 cut(s)	1866
FbaI	TGATCA	2 cut(s)	864, 1507
FblI	GTMKAC	1 cut(s)	1675
FokI	GGATG	6 cut(s)	642, 1090, 1172, 1200, 1415, 1468
FriOI	GRGCYC	1 cut(s)	160
FspBI	CTAG	5 cut(s)	680, 860, 1377, 1479, 1488
GlaI	GCGC	5 cut(s)	166, 898, 1752, 1758, 1786
GsaI	CCCAGC	1 cut(s)	1644
GsuI	CTGGAG	2 cut(s)	583, 1217
HaeII	RGCGCY	3 cut(s)	168, 900, 1788
HaeIII	GGCC	8 cut(s)	221, 290, 391, 803, 965, 1212, 1645, 1850
HgaI	GACGC	3 cut(s)	440, 453, 1330
HhaI	GCGC	5 cut(s)	167, 899, 1753, 1759, 1787
Hin1I	GRCGYC	4 cut(s)	483, 942, 1322, 1613
Hin1II	CATG	9 cut(s)	44, 595, 648, 750, 1063, 1237, 1349, 1673, 1858
Hin6I	GCGC	5 cut(s)	165, 897, 1751, 1757, 1785
HinP1I	GCGC	5 cut(s)	165, 897, 1751, 1757, 1785
HincII	GTYRAC	1 cut(s)	1676
HindII	GTYRAC	1 cut(s)	1676
Hpy166II	GTNNAC	4 cut(s)	567, 1033, 1221, 1676
Hpy188III	TCNNGA	8 cut(s)	374, 740, 760, 808, 994, 1133, 1511, 1855
Hpy8I	GTNNAC	4 cut(s)	567, 1033, 1221, 1676
Hpy99I	CGWCG	4 cut(s)	485, 518, 794, 1680
HpyAV	CCTTC	7 cut(s)	20, 353, 420, 1117, 1479, 1494, 1749
HpyCH4III	ACNGT	9 cut(s)	33, 518, 551, 564, 773, 1249, 1332, 1423, 1807
HpyCH4IV	ACGT	8 cut(s)	282, 483, 606, 756, 942, 1020, 1029, 1613
HpyF10VI	GCNNNNNNNGC	8 cut(s)	164, 227, 453, 795, 853, 905, 1754, 1787
HpyF3I	CTNAG	5 cut(s)	245, 273, 1134, 1770, 1794
HpySE526I	ACGT	8 cut(s)	282, 483, 606, 756, 942, 1020, 1029, 1613
Hsp92I	GRCGYC	4 cut(s)	483, 942, 1322, 1613
Hsp92II	CATG	9 cut(s)	44, 595, 648, 750, 1063, 1237, 1349, 1673, 1858
HspAI	GCGC	5 cut(s)	165, 897, 1751, 1757, 1785
Kpn2I	TCCGGA	1 cut(s)	739
Ksp22I	TGATCA	2 cut(s)	864, 1507
Kzo9I	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
LmnI	GCTCC	3 cut(s)	709, 1284, 1540
Lsp1109I	GCAGC	8 cut(s)	137, 446, 681, 927, 1199, 1288, 1735, 1777
LweI	GCATC	1 cut(s)	1639
MaeI	CTAG	5 cut(s)	680, 860, 1377, 1479, 1488
MaeII	ACGT	8 cut(s)	282, 483, 606, 756, 942, 1020, 1029, 1613
MaeIII	GTNAC	9 cut(s)	74, 558, 773, 1095, 1177, 1243, 1318, 1609, 1722
MalI	GATC	9 cut(s)	25, 590, 866, 992, 1139, 1356, 1509, 1530, 1681
MbiI	CCGCTC	1 cut(s)	813
MboI	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
MboII	GAAGA	3 cut(s)	950, 965, 1835
MfeI	CAATTG	1 cut(s)	1413
MflI	RGATCY	1 cut(s)	1528
MhlI	GDGCHC	5 cut(s)	160, 274, 399, 1563, 1575
MlsI	TGGCCA	2 cut(s)	1212, 1850
MluCI	AATT	5 cut(s)	110, 527, 542, 732, 1413
MluNI	TGGCCA	2 cut(s)	1212, 1850
MlyI	GAGTC	4 cut(s)	256, 1369, 1501, 1807
MmeI	TCCRAC	1 cut(s)	1231
Mox20I	TGGCCA	2 cut(s)	1212, 1850
MroI	TCCGGA	1 cut(s)	739
MroXI	GAANNNNTTC	1 cut(s)	542
MscI	TGGCCA	2 cut(s)	1212, 1850
MseI	TTAA	4 cut(s)	35, 735, 1037, 1873
MslI	CAYNNNNRTG	1 cut(s)	1350
Msp20I	TGGCCA	2 cut(s)	1212, 1850
MspA1I	CMGCKG	4 cut(s)	150, 1001, 1601, 1790
MspCI	CTTAAG	1 cut(s)	1036
MspR9I	CCNGG	2 cut(s)	292, 982
MunI	CAATTG	1 cut(s)	1413
MwoI	GCNNNNNNNGC	8 cut(s)	164, 227, 453, 795, 853, 905, 1754, 1787
NciI	CCSGG	2 cut(s)	292, 982
NdeI	CATATG	1 cut(s)	1866
NdeII	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
NheI	GCTAGC	1 cut(s)	1376
NlaIII	CATG	9 cut(s)	44, 595, 648, 750, 1063, 1237, 1349, 1673, 1858
NlaIV	GGNNCC	4 cut(s)	469, 821, 1515, 1530
NmuCI	GTSAC	7 cut(s)	74, 1095, 1177, 1243, 1318, 1609, 1722
NspI	RCATGY	2 cut(s)	44, 1237
NspV	TTCGAA	1 cut(s)	114
PagI	TCATGA	1 cut(s)	1854
PceI	AGGCCT	1 cut(s)	221
PciI	ACATGT	1 cut(s)	40
PcsI	WCGNNNNNNNCGW	3 cut(s)	510, 1026, 1328
PdmI	GAANNNNTTC	1 cut(s)	542
PfeI	GAWTC	6 cut(s)	116, 122, 743, 977, 1147, 1619
PflMI	CCANNNNNTGG	2 cut(s)	1065, 1707
PinAI	ACCGGT	1 cut(s)	305
PleI	GAGTC	4 cut(s)	255, 1368, 1500, 1807
PpsI	GAGTC	4 cut(s)	255, 1368, 1500, 1807
Ppu21I	YACGTR	1 cut(s)	1021
PpuMI	RGGWCCY	1 cut(s)	1513
PscI	ACATGT	1 cut(s)	40
PshBI	ATTAAT	1 cut(s)	735
Psp5II	RGGWCCY	1 cut(s)	1513
PspEI	GGTNACC	1 cut(s)	1722
PspFI	CCCAGC	1 cut(s)	1640
PspN4I	GGNNCC	4 cut(s)	469, 821, 1515, 1530
PspPI	GGNCC	3 cut(s)	820, 1513, 1643
PspPPI	RGGWCCY	1 cut(s)	1513
PstI	CTGCAG	1 cut(s)	155
PsuI	RGATCY	1 cut(s)	1528
PvuII	CAGCTG	3 cut(s)	150, 1001, 1601
RseI	CAYNNNNRTG	1 cut(s)	1350
SalI	GTCGAC	1 cut(s)	1674
SaqAI	TTAA	4 cut(s)	35, 735, 1037, 1873
Sau3AI	GATC	9 cut(s)	23, 588, 864, 990, 1137, 1354, 1507, 1528, 1679
Sau96I	GGNCC	3 cut(s)	820, 1513, 1643
ScaI	AGTACT	2 cut(s)	254, 299
SchI	GAGTC	4 cut(s)	256, 1369, 1501, 1807
ScrFI	CCNGG	2 cut(s)	292, 982
SduI	GDGCHC	5 cut(s)	160, 274, 399, 1563, 1575
SfaNI	GCATC	1 cut(s)	1639
SfcI	CTRYAG	3 cut(s)	151, 1002, 1153
SfuI	TTCGAA	1 cut(s)	114
SinI	GGWCC	2 cut(s)	820, 1513
SmiMI	CAYNNNNRTG	1 cut(s)	1350
SmlI	CTYRAG	3 cut(s)	372, 1036, 1568
SmoI	CTYRAG	3 cut(s)	372, 1036, 1568
SpeI	ACTAGT	2 cut(s)	679, 859
Sse9I	AATT	5 cut(s)	110, 527, 542, 732, 1413
SseBI	AGGCCT	1 cut(s)	221
SsiI	CCGC	9 cut(s)	231, 571, 813, 900, 1380, 1520, 1589, 1720, 1788
SspMI	CTAG	5 cut(s)	680, 860, 1377, 1479, 1488
StuI	AGGCCT	1 cut(s)	221
StyD4I	CCNGG	2 cut(s)	290, 980
StyI	CCWWGG	1 cut(s)	262
TaaI	ACNGT	9 cut(s)	33, 518, 551, 564, 773, 1249, 1332, 1423, 1807
TaiI	ACGT	8 cut(s)	285, 486, 609, 759, 945, 1023, 1032, 1616
TaqI	TCGA	6 cut(s)	114, 132, 513, 1238, 1583, 1675
TasI	AATT	5 cut(s)	110, 527, 542, 732, 1413
TatI	WGTACW	5 cut(s)	135, 252, 297, 564, 1032
TauI	GCSGC	3 cut(s)	233, 902, 1790
TfiI	GAWTC	6 cut(s)	116, 122, 743, 977, 1147, 1619
Tru1I	TTAA	4 cut(s)	35, 735, 1037, 1873
Tru9I	TTAA	4 cut(s)	35, 735, 1037, 1873
TscAI	CASTG	2 cut(s)	36, 181
TseFI	GTSAC	7 cut(s)	74, 1095, 1177, 1243, 1318, 1609, 1722
TseI	GCWGC	8 cut(s)	150, 434, 669, 915, 1187, 1276, 1748, 1790
Tsp45I	GTSAC	7 cut(s)	74, 1095, 1177, 1243, 1318, 1609, 1722
TspDTI	ATGAA	8 cut(s)	17, 735, 1076, 1445, 1461, 1472, 1658, 1871
TspGWI	ACGGA	2 cut(s)	989, 1206
TspRI	CASTG	2 cut(s)	36, 181
Van91I	CCANNNNNTGG	2 cut(s)	1065, 1707
Vha464I	CTTAAG	1 cut(s)	1036
VpaK11BI	GGWCC	2 cut(s)	820, 1513
VspI	ATTAAT	1 cut(s)	735
XapI	RAATTY	3 cut(s)	110, 527, 542
XceI	RCATGY	2 cut(s)	44, 1237
XmiI	GTMKAC	1 cut(s)	1675
XmnI	GAANNNNTTC	1 cut(s)	542
XspI	CTAG	5 cut(s)	680, 860, 1377, 1479, 1488
ZraI	GACGTC	3 cut(s)	484, 943, 1614
ZrmI	AGTACT	2 cut(s)	254, 299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.