Rw1G023710

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005230
Physical Location & Seq
Forward (+)
49701236 .. 49703620
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023710.1

Sequence Viewer

Length: 1431 bp
ATGGGATTGTTGAGAAGAAATCCCATGAAAACTCAAGTGATGAAATCAGTGTTAATAGTTGTTGTGGTGGCCTTAATACTAAGAGGGGGAGAGAGCAGAGGAGTAGCCAAGGCCCCCATAAAATCTGAGTCCTTTGAGTACTCTGCTATGAATTGCAGAGCTCACAGTGCTTCACTGACCGAGTTTGGAGGAGTTGGAGATGGAAAGACATCAAATACAAAGGCTTTTCAGACTGCAATCAGTACACTGAGCCAGTTTGCAACAGATGGAGGTGCTCAGCTTTTTGTTCCAGCAGGGAAATGGTTGGCAGGAAGCTTCAGCCTCACCAGCCATTTCACTCTTTATCTTCACAAGGATGCTGTTCTTCTTGCTTCTCAGGACTTGAATGAGTGGCCTGTGCTTAAGGCCCTTCCATCTTATGGCAGAGGAAGAGATGCAGCAGCTGGAAGGTACTCCAGTCTCATATTTGGAACAAATCTCACTGATGTCATTGTTACAGGAGACAATGGCACAATTGATGGTCAAGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTACCTCATTGAGATCATGTTCTCAAGTGACATCCAGATCTCAGATCTCACCCTCCTAAATTCTCCATCATGGAATGTTCATCCTGTCTATAGGATTACTATCATTGCTCCAATTTCGTCTCCAAACACCGATGGGATCAATCCAGATTCTTGCACAAATACGAGAATTGAAGACTGTTACATAGTCTCTGGTGATGATTGTGTAGCTGTTAAGAGTGGTTGGGATGAGTACGGCATATCATTTGGAATGCCTACAAAACAACTAGTGATCAGACGGCTCACATGCATTTCACCCTACAGTGCAACCATCGCATTGGGCAGCGAAATGTCAGGTGGGATCCAAGATGTCAGAGCCGAAGACATCGTGGCCATCAATACGGAATCAGCCGTCAGGATCAAGACAGCTGTAGGGAGAGGAGGGTATGTGAAAGACATATACGTCAGGAGAATGACACTGCACACAATGAAATGGGCATTTTGGATGACTGGTAATTATGGGTCACATGCTGATGGTAACTATGACAAAAATGCCTTTCCTGAGATCACTGGGATTAACTATAGAGACGTGGTGGCTGAAAATGTAACCATGGCTGCAAGATTGGAAGGCATTGCCGACCATCCATTTACCGGGATTTGCATGTCCAATGTGACCATTGGATTGGCAAAGAAGGCAAAGAAGCAGCCATGGACATGTACTGATATTCAGGGGATCACAAGCGGTGTGACGCCAACGCCCTGTGGTCTGCTGCCTGATCAAGGAACTGCTAAACCTGGTGGTTGTGATTTTCCAGCAGATGATATACCGATCGACATGGTAGAGATTAAAAAGTGTACTTATAGGAGTAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

476

Amino Acids

51.79

Weight (kDa)

7.95

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 152 - 428 3.4e-32 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1016
AccB7I CCANNNNNTGG 1 cut(s) 419
AciI CCGC 2 cut(s) 574, 1296
AclWI GGATC 5 cut(s) 722, 910, 923, 980, 1295
AcoI YGGCCR 1 cut(s) 945
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 1304
AfaI GTAC 6 cut(s) 140, 244, 452, 809, 1273, 1411
AfiI CCNNNNNNNGG 3 cut(s) 419, 1205, 1334
AflII CTTAAG 1 cut(s) 401
AflIII ACRYGT 1 cut(s) 1268
AgsI TTSAA 2 cut(s) 385, 749
AhlI ACTAGT 1 cut(s) 841
AjiI CACGTC 1 cut(s) 1144
AjnI CCWGG 1 cut(s) 1348
AluBI AGCT 7 cut(s) 161, 280, 315, 443, 562, 785, 983
AluI AGCT 7 cut(s) 161, 280, 315, 443, 562, 785, 983
Alw21I GWGCWC 2 cut(s) 163, 277
Alw26I GTCTC 5 cut(s) 464, 495, 702, 769, 1134
AlwI GGATC 5 cut(s) 722, 910, 923, 980, 1295
AlwNI CAGNNNCTG 1 cut(s) 443
AoxI GGCC 5 cut(s) 69, 111, 392, 405, 945
ApeKI GCWGC 6 cut(s) 437, 440, 897, 1169, 1258, 1324
ApoI RAATTY 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 112, 406
AsuC2I CCSGG 1 cut(s) 1207
AsuHPI GGTGA 5 cut(s) 316, 539, 619, 782, 861
BaeI ACNNNNGTAYC 2 cut(s) 442, 475
BalI TGGCCA 1 cut(s) 947
BamHI GGATCC 1 cut(s) 915
BanII GRGCYC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 2 cut(s) 756, 942
Bbv12I GWGCWC 2 cut(s) 163, 277
BbvI GCAGC 6 cut(s) 449, 452, 909, 1156, 1270, 1311
BceAI ACGGC 3 cut(s) 826, 869, 950
BciT130I CCWGG 1 cut(s) 1350
BclI TGATCA 2 cut(s) 846, 1330
BcnI CCSGG 1 cut(s) 1207
BcoDI GTCTC 5 cut(s) 464, 495, 702, 769, 1134
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 1 cut(s) 842
BfmI CTRYAG 4 cut(s) 667, 874, 984, 1135
BfrI CTTAAG 1 cut(s) 401
BglII AGATCT 2 cut(s) 615, 622
BisI GCNGC 6 cut(s) 438, 441, 898, 1170, 1259, 1325
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 6 cut(s) 439, 442, 899, 1171, 1260, 1326
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 2 cut(s) 1207, 1350
BmgBI CACGTC 1 cut(s) 1144
BmgT120I GGNCC 2 cut(s) 112, 406
BmiI GGNNCC 2 cut(s) 114, 917
BmrFI CCNGG 2 cut(s) 1207, 1350
BmrI ACTGGG 1 cut(s) 1134
BmsI GCATC 2 cut(s) 346, 424
BmuI ACTGGG 1 cut(s) 1134
BpiI GAAGAC 2 cut(s) 756, 942
BpmI CTGGAG 1 cut(s) 439
Bpu1102I GCTNAGC 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 18, 586
BpuMI CCSGG 1 cut(s) 1207
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 1304
BsaJI CCNNGG 3 cut(s) 108, 1164, 1262
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 1205, 1334
Bse1I ACTGG 4 cut(s) 253, 456, 1069, 1129
Bse3DI GCAATG 2 cut(s) 681, 1185
Bse8I GATNNNNATC 1 cut(s) 677
BseBI CCWGG 1 cut(s) 1350
BseDI CCNNGG 3 cut(s) 108, 1164, 1262
BseGI GGATG 6 cut(s) 361, 609, 658, 808, 1065, 1195
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 3 cut(s) 419, 1205, 1334
BseMI GCAATG 2 cut(s) 681, 1185
BseMII CTCAG 6 cut(s) 117, 239, 290, 389, 633, 1107
BseNI ACTGG 4 cut(s) 253, 456, 1069, 1129
BseRI GAGGAG 3 cut(s) 114, 204, 1008
BseXI GCAGC 6 cut(s) 449, 452, 909, 1156, 1270, 1311
BsgI GTGCAG 1 cut(s) 1019
Bsh1285I CGRYCG 1 cut(s) 1386
BshFI GGCC 5 cut(s) 71, 113, 394, 407, 947
BsiEI CGRYCG 1 cut(s) 1386
BsiHKAI GWGCWC 2 cut(s) 163, 277
BsiSI CCGG 1 cut(s) 1206
BslI CCNNNNNNNGG 3 cut(s) 419, 1205, 1334
BsmAI GTCTC 5 cut(s) 464, 495, 702, 769, 1134
BsmBI CGTCTC 2 cut(s) 702, 1134
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 5 cut(s) 71, 113, 394, 407, 947
Bsp1286I GDGCHC 2 cut(s) 163, 277
Bsp1720I GCTNAGC 1 cut(s) 276
Bsp19I CCATGG 2 cut(s) 1164, 1262
BspACI CCGC 2 cut(s) 574, 1296
BspANI GGCC 5 cut(s) 71, 113, 394, 407, 947
BspCNI CTCAG 6 cut(s) 118, 240, 289, 388, 632, 1108
BspLI GGNNCC 2 cut(s) 114, 917
BspPI GGATC 5 cut(s) 722, 910, 923, 980, 1295
BspTI CTTAAG 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 681, 1185
BsrI ACTGG 4 cut(s) 253, 456, 1069, 1129
BssECI CCNNGG 3 cut(s) 108, 1164, 1262
BssNI GRCGYC 1 cut(s) 1304
BssT1I CCWWGG 3 cut(s) 108, 1164, 1262
Bst2UI CCWGG 1 cut(s) 1350
Bst4CI ACNGT 4 cut(s) 167, 546, 755, 878
Bst6I CTCTTC 1 cut(s) 424
BstACI GRCGYC 1 cut(s) 1304
BstAFI CTTAAG 1 cut(s) 401
BstDEI CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1116
BstDSI CCRYGG 2 cut(s) 1164, 1262
BstENI CCTNNNNNAGG 1 cut(s) 1332
BstF5I GGATG 6 cut(s) 361, 609, 658, 808, 1065, 1195
BstMAI GTCTC 5 cut(s) 464, 495, 702, 769, 1134
BstMCI CGRYCG 1 cut(s) 1386
BstMWI GCNNNNNNNGC 4 cut(s) 167, 327, 887, 1247
BstNI CCWGG 1 cut(s) 1350
BstNSI RCATGY 4 cut(s) 864, 1085, 1219, 1272
BstSCI CCNGG 2 cut(s) 1205, 1348
BstSFI CTRYAG 4 cut(s) 667, 874, 984, 1135
BstV1I GCAGC 6 cut(s) 449, 452, 909, 1156, 1270, 1311
BstV2I GAAGAC 2 cut(s) 756, 942
BstX2I RGATCY 3 cut(s) 615, 622, 915
BstXI CCANNNNNNTGG 2 cut(s) 892, 1237
BstYI RGATCY 3 cut(s) 615, 622, 915
BsuRI GGCC 5 cut(s) 71, 113, 394, 407, 947
BtgI CCRYGG 2 cut(s) 1164, 1262
BtgZI GCGATG 1 cut(s) 871
BtrI CACGTC 1 cut(s) 1144
BtsCI GGATG 6 cut(s) 361, 609, 658, 808, 1065, 1195
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 8 cut(s) 54, 172, 173, 245, 480, 883, 1031, 1122
CaiI CAGNNNCTG 1 cut(s) 443
Cfr13I GGNCC 2 cut(s) 112, 406
CseI GACGC 1 cut(s) 1312
CsiI ACCWGGT 1 cut(s) 1348
Csp6I GTAC 6 cut(s) 139, 243, 451, 808, 1272, 1410
CviQI GTAC 6 cut(s) 139, 243, 451, 808, 1272, 1410
DdeI CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1116
DrdI GACNNNNNNGTC 1 cut(s) 1016
DseDI GACNNNNNNGTC 1 cut(s) 1016
EaeI YGGCCR 1 cut(s) 945
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
Ecl136II GAGCTC 1 cut(s) 161
Eco130I CCWWGG 3 cut(s) 108, 1164, 1262
Eco24I GRGCYC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 1332
EcoO109I RGGNCCY 2 cut(s) 112, 406
EcoRII CCWGG 1 cut(s) 1348
EcoT14I CCWWGG 3 cut(s) 108, 1164, 1262
EcoT22I ATGCAT 1 cut(s) 866
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 3 cut(s) 108, 1164, 1262
Esp3I CGTCTC 2 cut(s) 702, 1134
FauI CCCGC 1 cut(s) 581
FbaI TGATCA 2 cut(s) 846, 1330
Fnu4HI GCNGC 6 cut(s) 438, 441, 898, 1170, 1259, 1325
FokI GGATG 6 cut(s) 368, 596, 645, 815, 1072, 1182
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 6 cut(s) 438, 441, 898, 1170, 1259, 1325
FspBI CTAG 1 cut(s) 842
GluI GCNGC 6 cut(s) 438, 441, 898, 1170, 1259, 1325
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 5 cut(s) 71, 113, 394, 407, 947
HapII CCGG 1 cut(s) 1206
HgaI GACGC 1 cut(s) 1312
Hin1I GRCGYC 1 cut(s) 1304
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 3 cut(s) 128, 725, 959
HpaII CCGG 1 cut(s) 1206
HphI GGTGA 5 cut(s) 316, 539, 619, 782, 861
Hpy166II GTNNAC 2 cut(s) 245, 1410
Hpy188I TCNGA 5 cut(s) 127, 231, 622, 851, 929
Hpy188III TCNNGA 7 cut(s) 377, 613, 722, 970, 976, 1021, 1115
Hpy8I GTNNAC 2 cut(s) 245, 1410
HpyAV CCTTC 4 cut(s) 419, 441, 1175, 1240
HpyCH4III ACNGT 4 cut(s) 167, 546, 755, 878
HpyCH4IV ACGT 2 cut(s) 1017, 1143
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 327, 887, 1247
HpyF3I CTNAG 7 cut(s) 80, 126, 248, 276, 375, 619, 1116
HpySE526I ACGT 2 cut(s) 1017, 1143
Hsp92I GRCGYC 1 cut(s) 1304
Ksp22I TGATCA 2 cut(s) 846, 1330
LmnI GCTCC 1 cut(s) 691
Lsp1109I GCAGC 6 cut(s) 449, 452, 909, 1156, 1270, 1311
LweI GCATC 2 cut(s) 346, 424
MabI ACCWGGT 1 cut(s) 1348
MaeI CTAG 1 cut(s) 842
MaeII ACGT 2 cut(s) 1017, 1143
MaeIII GTNAC 8 cut(s) 493, 605, 755, 1077, 1091, 1159, 1225, 1300
MboII GAAGA 7 cut(s) 27, 338, 356, 441, 568, 761, 947
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 3 cut(s) 615, 622, 915
MhlI GDGCHC 2 cut(s) 163, 277
MlsI TGGCCA 1 cut(s) 947
MluCI AATT 6 cut(s) 151, 513, 637, 690, 744, 1069
MluNI TGGCCA 1 cut(s) 947
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 175
Mox20I TGGCCA 1 cut(s) 947
Mph1103I ATGCAT 1 cut(s) 866
MscI TGGCCA 1 cut(s) 947
MseI TTAA 6 cut(s) 53, 74, 402, 789, 1131, 1401
MslI CAYNNNNRTG 3 cut(s) 354, 1086, 1267
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 443, 983
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 1 cut(s) 1206
MspR9I CCNGG 2 cut(s) 1207, 1350
MunI CAATTG 1 cut(s) 513
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 1350
MwoI GCNNNNNNNGC 4 cut(s) 167, 327, 887, 1247
NciI CCSGG 1 cut(s) 1207
NcoI CCATGG 2 cut(s) 1164, 1262
NlaIV GGNNCC 2 cut(s) 114, 917
NmuCI GTSAC 4 cut(s) 605, 1077, 1225, 1300
NsiI ATGCAT 1 cut(s) 866
NspI RCATGY 4 cut(s) 864, 1085, 1219, 1272
PciI ACATGT 1 cut(s) 1268
PctI GAATGC 1 cut(s) 831
PfeI GAWTC 2 cut(s) 725, 959
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 6 cut(s) 439, 442, 899, 1171, 1260, 1326
Ple19I CGATCG 1 cut(s) 1386
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PscI ACATGT 1 cut(s) 1268
Psp124BI GAGCTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 1348
PspGI CCWGG 1 cut(s) 1348
PspN4I GGNNCC 2 cut(s) 114, 917
PspPI GGNCC 2 cut(s) 112, 406
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 3 cut(s) 615, 622, 915
PvuI CGATCG 1 cut(s) 1386
PvuII CAGCTG 2 cut(s) 443, 983
RsaI GTAC 6 cut(s) 140, 244, 452, 809, 1273, 1411
RsaNI GTAC 6 cut(s) 139, 243, 451, 808, 1272, 1410
RseI CAYNNNNRTG 3 cut(s) 354, 1086, 1267
SacI GAGCTC 1 cut(s) 163
SaqAI TTAA 6 cut(s) 53, 74, 402, 789, 1131, 1401
SatI GCNGC 6 cut(s) 438, 441, 898, 1170, 1259, 1325
Sau96I GGNCC 2 cut(s) 112, 406
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 1207, 1350
SduI GDGCHC 2 cut(s) 163, 277
SexAI ACCWGGT 1 cut(s) 1348
SfaNI GCATC 2 cut(s) 346, 424
SfcI CTRYAG 4 cut(s) 667, 874, 984, 1135
SmiMI CAYNNNNRTG 3 cut(s) 354, 1086, 1267
SmlI CTYRAG 3 cut(s) 33, 401, 601
SmoI CTYRAG 3 cut(s) 33, 401, 601
SpeI ACTAGT 1 cut(s) 841
Sse9I AATT 6 cut(s) 151, 513, 637, 690, 744, 1069
SsiI CCGC 2 cut(s) 574, 1296
SspMI CTAG 1 cut(s) 842
SstI GAGCTC 1 cut(s) 163
StyD4I CCNGG 2 cut(s) 1205, 1348
StyI CCWWGG 3 cut(s) 108, 1164, 1262
TaaI ACNGT 4 cut(s) 167, 546, 755, 878
TaiI ACGT 2 cut(s) 1020, 1146
TaqI TCGA 1 cut(s) 1386
TaqII GACCGA 1 cut(s) 194
TasI AATT 6 cut(s) 151, 513, 637, 690, 744, 1069
TatI WGTACW 4 cut(s) 138, 242, 1271, 1409
TfiI GAWTC 2 cut(s) 725, 959
Tru1I TTAA 6 cut(s) 53, 74, 402, 789, 1131, 1401
Tru9I TTAA 6 cut(s) 53, 74, 402, 789, 1131, 1401
TscAI CASTG 8 cut(s) 54, 172, 180, 252, 487, 883, 1038, 1129
TseFI GTSAC 4 cut(s) 605, 1077, 1225, 1300
TseI GCWGC 6 cut(s) 437, 440, 897, 1169, 1258, 1324
Tsp45I GTSAC 4 cut(s) 605, 1077, 1225, 1300
TspDTI ATGAA 5 cut(s) 41, 56, 164, 647, 1058
TspGWI ACGGA 1 cut(s) 971
TspRI CASTG 8 cut(s) 54, 172, 180, 252, 487, 883, 1038, 1129
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 401
XagI CCTNNNNNAGG 1 cut(s) 1332
XapI RAATTY 1 cut(s) 637
XceI RCATGY 4 cut(s) 864, 1085, 1219, 1272
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
XspI CTAG 1 cut(s) 842
ZrmI AGTACT 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.