RLG00000028048

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005230
Physical Location & Seq
Reverse (-)
18067677 .. 18070074
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028048

Sequence Viewer

Length: 1464 bp
ATGGGATTGTTGAGAAGAAATCCCATGAAAACTCAAGTGATGAAATCAGTGTTAATAGTTGTTGTGGTGGCCTTAATACTAAGAGGGGGAGAGAGCAGAGGAGTAGCCAAGGCCAAGCCTAAGGCCCCCATAACATCTGAGTCCTTTGAGTACTCTGCTATGAATTGCAGAGCTCACAGTGCTTCACTGACCGAGTTTGGAGGAGTTGGAGATGGAAAGACATCAAATACAAAGGCTTTTCAGACTGCAATCAGTAAGCTGAGCCAGTTTGCAACAGATGGAGGTGCTCAGCTTTTTGTTCCGGCTGGGAAATGGTTGACAGGAAGCTTCAGCCTCACCAGCCATTTCACTCTTTATCTTCACAAGGATGCTGTTCTTCTTGCTTCTCAGGACTTGAATGAGTGGCCTGTGCTTAAAGCCCTTCCATCTTATGGTAGAGGAAGAGATGCAGCAGCTGGAAGGTTCTCCAGTCTCATATTTGGAACAAATCTCACTGATGTTATTGTTACAGGAGACAATGGCACAATTGATGGTCAAGGTGAGTTCTGGTGGCAACAGTTCCACAAGAAGAAGCTAAAATACACCCGCCCTTACCTGATTGAGATCATGTTCTCAAGTGATATCCAGATCTCAGATCTCACCCTCCTAAATTCTCCATCATGGAATGTTCATCCTGTCTATAGCAGTAACATTCTTGTGCAAGGGATTACTATCATTGCTCCAATTTCGTCTCCAAACACTGATGGGATCAATCCAGATTCTTGCACAAATACTAGAATTGAAGACTGTTACATAGTCTCTGGTGATGATTGTGTTGCTGTTAAGAGTGGCTGGGATGAGTATGGCATATCATTTGGAATGCCTACAAAACAACTAGTGATCAGACGGCTCACATGCATTTCACCCTACAGTGCAACCATCGCATTGGGCAGCGAAATGTCAGGTGGGATCCAAGACGTCAGAGCTGAAGACATCGTGGCCATCAATACCGAATCAGCCGTCAGGATCAAGACAGCTGTAGGGAGGGGAGGGTATGTGAAAGACATCTATGTAAGGAGAATGACACTGCACACAATGAAATGGGCATTTTGGATGACTGGTAATTATGGGTCACATGCTGATGGTAACTATGACAAAAATGCCTTTCCTGAGATCACCGGGATTAACTATAGAGACGTGGTGGCTGAAAATGTAACCATGGCTGCAAGATTGGAGGGCATTGCCGACCATCCATTTACCGGGATTTGCATGTCCAATGTGACTATTGGATTGGCAAAGAAGGCAAAGAAGCAGCCATGGACATGTACTGATATTCAGGGGATCACAAGCGGTGTGACGCCAACGCCCTGTGGTCTGCTGCCTGATCAAGGAACTGCTAAACCTGGTGGTTGTGATTTTCCAGAAGATGATATACCGATCGACATGGTCGAGATTAAAAAGTGTACTTATAGGAGTAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

488

Amino Acids

52.93

Weight (kDa)

7.95

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 156 - 439 5.7e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 960
AccB7I CCANNNNNTGG 1 cut(s) 431
AciI CCGC 2 cut(s) 586, 1329
AclWI GGATC 5 cut(s) 755, 943, 956, 1013, 1328
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 2 cut(s) 313, 987
AcyI GRCGYC 2 cut(s) 957, 1337
AfaI GTAC 3 cut(s) 152, 1306, 1444
AfiI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
AflIII ACRYGT 1 cut(s) 1301
AgsI TTSAA 2 cut(s) 397, 782
AhlI ACTAGT 1 cut(s) 874
AjiI CACGTC 1 cut(s) 1177
AjnI CCWGG 1 cut(s) 1381
AluBI AGCT 8 cut(s) 173, 259, 292, 327, 455, 574, 965, 1016
AluI AGCT 8 cut(s) 173, 259, 292, 327, 455, 574, 965, 1016
Alw21I GWGCWC 2 cut(s) 175, 289
Alw26I GTCTC 5 cut(s) 476, 507, 735, 802, 1167
AlwI GGATC 5 cut(s) 755, 943, 956, 1013, 1328
AlwNI CAGNNNCTG 1 cut(s) 455
AoxI GGCC 5 cut(s) 69, 111, 123, 404, 978
ApeKI GCWGC 6 cut(s) 449, 452, 930, 1202, 1291, 1357
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
AspS9I GGNCC 1 cut(s) 124
AsuC2I CCSGG 2 cut(s) 1159, 1240
AsuHPI GGTGA 6 cut(s) 328, 551, 631, 815, 894, 1147
AxyI CCTNAGG 1 cut(s) 120
BalI TGGCCA 1 cut(s) 980
BamHI GGATCC 1 cut(s) 948
BanII GRGCYC 1 cut(s) 175
BarI GAAGNNNNNNTAC 4 cut(s) 563, 595, 1395, 1427
BbsI GAAGAC 2 cut(s) 789, 975
Bbv12I GWGCWC 2 cut(s) 175, 289
BbvI GCAGC 6 cut(s) 461, 464, 942, 1189, 1303, 1344
BceAI ACGGC 2 cut(s) 902, 983
BciT130I CCWGG 1 cut(s) 1383
BclI TGATCA 2 cut(s) 879, 1363
BcnI CCSGG 2 cut(s) 1159, 1240
BcoDI GTCTC 5 cut(s) 476, 507, 735, 802, 1167
BcuI ACTAGT 1 cut(s) 874
BfaI CTAG 2 cut(s) 774, 875
BfmI CTRYAG 4 cut(s) 679, 907, 1017, 1168
BglII AGATCT 2 cut(s) 627, 634
BisI GCNGC 6 cut(s) 450, 453, 931, 1203, 1292, 1358
BlpI GCTNAGC 2 cut(s) 260, 288
BlsI GCNGC 6 cut(s) 451, 454, 932, 1204, 1293, 1359
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 1159, 1240, 1383
BmgBI CACGTC 1 cut(s) 1177
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 126, 950
BmrFI CCNGG 3 cut(s) 1159, 1240, 1383
BmsI GCATC 2 cut(s) 358, 436
BpiI GAAGAC 2 cut(s) 789, 975
BpmI CTGGAG 1 cut(s) 451
Bpu1102I GCTNAGC 2 cut(s) 260, 288
BpuEI CTTGAG 2 cut(s) 18, 598
BpuMI CCSGG 2 cut(s) 1159, 1240
BsaBI GATNNNNATC 2 cut(s) 602, 710
BsaHI GRCGYC 2 cut(s) 957, 1337
BsaJI CCNNGG 3 cut(s) 108, 1197, 1295
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
Bse1I ACTGG 3 cut(s) 265, 468, 1102
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 2 cut(s) 714, 1218
Bse8I GATNNNNATC 2 cut(s) 602, 710
BseBI CCWGG 1 cut(s) 1383
BseDI CCNNGG 3 cut(s) 108, 1197, 1295
BseGI GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BseJI GATNNNNATC 2 cut(s) 602, 710
BseLI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
BseMI GCAATG 2 cut(s) 714, 1218
BseMII CTCAG 6 cut(s) 129, 251, 302, 401, 645, 1140
BseNI ACTGG 3 cut(s) 265, 468, 1102
BseRI GAGGAG 2 cut(s) 114, 216
BseXI GCAGC 6 cut(s) 461, 464, 942, 1189, 1303, 1344
BseYI CCCAGC 2 cut(s) 305, 831
BsgI GTGCAG 1 cut(s) 1052
Bsh1285I CGRYCG 1 cut(s) 1419
BshFI GGCC 5 cut(s) 71, 113, 125, 406, 980
BsiEI CGRYCG 1 cut(s) 1419
BsiHKAI GWGCWC 2 cut(s) 175, 289
BsiSI CCGG 3 cut(s) 302, 1158, 1239
BslI CCNNNNNNNGG 3 cut(s) 431, 1238, 1367
BsmAI GTCTC 5 cut(s) 476, 507, 735, 802, 1167
BsmBI CGTCTC 2 cut(s) 735, 1167
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 5 cut(s) 71, 113, 125, 406, 980
Bsp1286I GDGCHC 2 cut(s) 175, 289
Bsp1720I GCTNAGC 2 cut(s) 260, 288
Bsp19I CCATGG 2 cut(s) 1197, 1295
BspACI CCGC 2 cut(s) 586, 1329
BspANI GGCC 5 cut(s) 71, 113, 125, 406, 980
BspCNI CTCAG 6 cut(s) 130, 252, 301, 400, 644, 1141
BspLI GGNNCC 2 cut(s) 126, 950
BspPI GGATC 5 cut(s) 755, 943, 956, 1013, 1328
BsrDI GCAATG 2 cut(s) 714, 1218
BsrI ACTGG 3 cut(s) 265, 468, 1102
BssECI CCNNGG 3 cut(s) 108, 1197, 1295
BssNI GRCGYC 2 cut(s) 957, 1337
BssT1I CCWWGG 3 cut(s) 108, 1197, 1295
Bst2UI CCWGG 1 cut(s) 1383
Bst4CI ACNGT 4 cut(s) 179, 558, 788, 911
Bst6I CTCTTC 1 cut(s) 436
BstACI GRCGYC 2 cut(s) 957, 1337
BstDEI CTNAG 8 cut(s) 80, 120, 138, 260, 288, 387, 631, 1149
BstDSI CCRYGG 2 cut(s) 1197, 1295
BstENI CCTNNNNNAGG 1 cut(s) 1365
BstF5I GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BstMAI GTCTC 5 cut(s) 476, 507, 735, 802, 1167
BstMCI CGRYCG 1 cut(s) 1419
BstMWI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
BstNI CCWGG 1 cut(s) 1383
BstNSI RCATGY 4 cut(s) 897, 1118, 1252, 1305
BstSCI CCNGG 3 cut(s) 1157, 1238, 1381
BstSFI CTRYAG 4 cut(s) 679, 907, 1017, 1168
BstV1I GCAGC 6 cut(s) 461, 464, 942, 1189, 1303, 1344
BstV2I GAAGAC 2 cut(s) 789, 975
BstX2I RGATCY 3 cut(s) 627, 634, 948
BstXI CCANNNNNNTGG 1 cut(s) 925
BstYI RGATCY 3 cut(s) 627, 634, 948
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 5 cut(s) 71, 113, 125, 406, 980
BtgI CCRYGG 2 cut(s) 1197, 1295
BtgZI GCGATG 1 cut(s) 904
BtrI CACGTC 1 cut(s) 1177
BtsCI GGATG 5 cut(s) 373, 670, 841, 1098, 1228
BtsI GCAGTG 1 cut(s) 1064
BtsIMutI CAGTG 7 cut(s) 54, 184, 185, 492, 738, 916, 1064
CaiI CAGNNNCTG 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 124
CseI GACGC 1 cut(s) 1345
CsiI ACCWGGT 1 cut(s) 1381
Csp6I GTAC 3 cut(s) 151, 1305, 1443
CviQI GTAC 3 cut(s) 151, 1305, 1443
DdeI CTNAG 8 cut(s) 80, 120, 138, 260, 288, 387, 631, 1149
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 3 cut(s) 108, 1197, 1295
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 313, 987
Eco81I CCTNAGG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 173
EcoNI CCTNNNNNAGG 1 cut(s) 1365
EcoO109I RGGNCCY 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 1381
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 3 cut(s) 108, 1197, 1295
EcoT22I ATGCAT 1 cut(s) 899
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 3 cut(s) 108, 1197, 1295
Esp3I CGTCTC 2 cut(s) 735, 1167
FauI CCCGC 1 cut(s) 593
FbaI TGATCA 2 cut(s) 879, 1363
Fnu4HI GCNGC 6 cut(s) 450, 453, 931, 1203, 1292, 1358
FokI GGATG 5 cut(s) 380, 657, 848, 1105, 1215
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 6 cut(s) 450, 453, 931, 1203, 1292, 1358
FspBI CTAG 2 cut(s) 774, 875
GluI GCNGC 6 cut(s) 450, 453, 931, 1203, 1292, 1358
GsaI CCCAGC 2 cut(s) 309, 835
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 5 cut(s) 71, 113, 125, 406, 980
HapII CCGG 3 cut(s) 302, 1158, 1239
HgaI GACGC 1 cut(s) 1345
Hin1I GRCGYC 2 cut(s) 957, 1337
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 3 cut(s) 140, 758, 992
HpaII CCGG 3 cut(s) 302, 1158, 1239
HphI GGTGA 6 cut(s) 328, 551, 631, 815, 894, 1147
Hpy166II GTNNAC 2 cut(s) 318, 1443
Hpy188I TCNGA 5 cut(s) 139, 243, 634, 884, 962
Hpy188III TCNNGA 8 cut(s) 389, 625, 755, 1003, 1009, 1148, 1400, 1429
Hpy8I GTNNAC 2 cut(s) 318, 1443
HpyAV CCTTC 3 cut(s) 431, 453, 1273
HpyCH4III ACNGT 4 cut(s) 179, 558, 788, 911
HpyCH4IV ACGT 2 cut(s) 957, 1176
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
HpyF3I CTNAG 8 cut(s) 80, 120, 138, 260, 288, 387, 631, 1149
HpySE526I ACGT 2 cut(s) 957, 1176
Hsp92I GRCGYC 2 cut(s) 957, 1337
Ksp22I TGATCA 2 cut(s) 879, 1363
LmnI GCTCC 1 cut(s) 724
Lsp1109I GCAGC 6 cut(s) 461, 464, 942, 1189, 1303, 1344
LweI GCATC 2 cut(s) 358, 436
MabI ACCWGGT 1 cut(s) 1381
MaeI CTAG 2 cut(s) 774, 875
MaeII ACGT 2 cut(s) 957, 1176
MaeIII GTNAC 8 cut(s) 505, 686, 788, 1110, 1124, 1192, 1258, 1333
MboII GAAGA 8 cut(s) 27, 350, 368, 453, 580, 794, 980, 1415
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 3 cut(s) 627, 634, 948
MhlI GDGCHC 2 cut(s) 175, 289
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 187
Mox20I TGGCCA 1 cut(s) 980
Mph1103I ATGCAT 1 cut(s) 899
MscI TGGCCA 1 cut(s) 980
MseI TTAA 6 cut(s) 53, 74, 414, 822, 1164, 1434
MslI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
Msp20I TGGCCA 1 cut(s) 980
MspA1I CMGCKG 2 cut(s) 455, 1016
MspI CCGG 3 cut(s) 302, 1158, 1239
MspR9I CCNGG 3 cut(s) 1159, 1240, 1383
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 1 cut(s) 1383
MwoI GCNNNNNNNGC 4 cut(s) 179, 339, 920, 1280
NciI CCSGG 2 cut(s) 1159, 1240
NcoI CCATGG 2 cut(s) 1197, 1295
NlaIV GGNNCC 2 cut(s) 126, 950
NmuCI GTSAC 3 cut(s) 1110, 1258, 1333
NsiI ATGCAT 1 cut(s) 899
NspI RCATGY 4 cut(s) 897, 1118, 1252, 1305
PciI ACATGT 1 cut(s) 1301
PcsI WCGNNNNNNNCGW 1 cut(s) 1425
PctI GAATGC 1 cut(s) 864
PfeI GAWTC 2 cut(s) 758, 992
PflFI GACNNNGTC 1 cut(s) 1424
PflMI CCANNNNNTGG 1 cut(s) 431
PkrI GCNGC 6 cut(s) 451, 454, 932, 1204, 1293, 1359
Ple19I CGATCG 1 cut(s) 1419
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PscI ACATGT 1 cut(s) 1301
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 1381
PspFI CCCAGC 2 cut(s) 305, 831
PspGI CCWGG 1 cut(s) 1381
PspN4I GGNNCC 2 cut(s) 126, 950
PspPI GGNCC 1 cut(s) 124
PstNI CAGNNNCTG 1 cut(s) 455
PsuI RGATCY 3 cut(s) 627, 634, 948
PsyI GACNNNGTC 1 cut(s) 1424
PvuI CGATCG 1 cut(s) 1419
PvuII CAGCTG 2 cut(s) 455, 1016
RsaI GTAC 3 cut(s) 152, 1306, 1444
RsaNI GTAC 3 cut(s) 151, 1305, 1443
RseI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 6 cut(s) 53, 74, 414, 822, 1164, 1434
SatI GCNGC 6 cut(s) 450, 453, 931, 1203, 1292, 1358
Sau96I GGNCC 1 cut(s) 124
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 3 cut(s) 1159, 1240, 1383
SduI GDGCHC 2 cut(s) 175, 289
SexAI ACCWGGT 1 cut(s) 1381
SfaNI GCATC 2 cut(s) 358, 436
SfcI CTRYAG 4 cut(s) 679, 907, 1017, 1168
SmiMI CAYNNNNRTG 4 cut(s) 366, 695, 1119, 1300
SmlI CTYRAG 2 cut(s) 33, 613
SmoI CTYRAG 2 cut(s) 33, 613
SpeI ACTAGT 1 cut(s) 874
Sse9I AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
SsiI CCGC 2 cut(s) 586, 1329
SspMI CTAG 2 cut(s) 774, 875
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 3 cut(s) 1157, 1238, 1381
StyI CCWWGG 3 cut(s) 108, 1197, 1295
TaaI ACNGT 4 cut(s) 179, 558, 788, 911
TaiI ACGT 2 cut(s) 960, 1179
TaqI TCGA 2 cut(s) 1419, 1428
TaqII GACCGA 1 cut(s) 206
TasI AATT 6 cut(s) 163, 525, 649, 723, 777, 1102
TatI WGTACW 3 cut(s) 150, 1304, 1442
TfiI GAWTC 2 cut(s) 758, 992
Tru1I TTAA 6 cut(s) 53, 74, 414, 822, 1164, 1434
Tru9I TTAA 6 cut(s) 53, 74, 414, 822, 1164, 1434
TscAI CASTG 7 cut(s) 54, 184, 192, 499, 745, 916, 1071
TseFI GTSAC 3 cut(s) 1110, 1258, 1333
TseI GCWGC 6 cut(s) 449, 452, 930, 1202, 1291, 1357
Tsp45I GTSAC 3 cut(s) 1110, 1258, 1333
TspDTI ATGAA 5 cut(s) 41, 56, 176, 659, 1091
TspRI CASTG 7 cut(s) 54, 184, 192, 499, 745, 916, 1071
Tth111I GACNNNGTC 1 cut(s) 1424
Van91I CCANNNNNTGG 1 cut(s) 431
XagI CCTNNNNNAGG 1 cut(s) 1365
XapI RAATTY 1 cut(s) 649
XceI RCATGY 4 cut(s) 897, 1118, 1252, 1305
XspI CTAG 2 cut(s) 774, 875
ZraI GACGTC 1 cut(s) 958
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.