MD01G1069000.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005230
Physical Location & Seq
Forward (+)
17312361 .. 17313291
931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1069000.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGGGGTCCAATTAAATTCTTGCACCAATACAAGAATTGAAGACTGCTACATAGTCTCTGGGGATGATTGTGTAGCTGTTAAGAGCGGTTGGGACGAGTACGGAATTGCATTTGGAATGCCTACAAAACAACTAGTAGTCCGACGTCTCACATGCATTTCGCCCTACAGCGCCACCATTGCCCTAGGCAGTGAAATGTCCGGCGGGATCCAAGACGTCCGAGCTGAAGACATAGTGGCCATCCATACCGAATCGGGTGTCCGGATCAAGACCGCCGTAGGGCGAGGAGGGTACGTGAAAGACATATACGCGAGGAGATTCACCATGCACACAATGAAATGGGCGTTTTGGATGACCGGCAATTACGGGTCACATGCTGATGACAAATATGATAAAAATGCACTGCCTGAGATCAAAAATATTAACTACAGGGACATGGTGGCCGATAATGTCACAATGGCTGCAAGGTTGGAAGGCATTGCGGACCACCCGTTTACCGGGATTTGCATCTCGAATGTGACGATTGGACTGGCGGTGAAGGCGAAGAAGCAGCCATGGACGTGCACCGACATTGAGGGGATCACGAGCGGCGTGACTCCTCGCCCTTGTGATCTGCTGCCGGATCAGGGTGCTGAGAAAGCCAAAGGTTGTGATTTTCCTGCAGATGATCTGCCGATTGACCGGGTAGAGCTCAAACAGTGTACATATAGGATGAGCTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

242

Amino Acids

26.45

Weight (kDa)

5.83

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 192 6.4e-14 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 148, 219
AccBSI CCGCTC 2 cut(s) 87, 588
AccII CGCG 1 cut(s) 311
AccIII TCCGGA 1 cut(s) 261
AciI CCGC 6 cut(s) 87, 204, 273, 482, 533, 588
AclWI GGATC 5 cut(s) 202, 215, 272, 587, 630
AcoI YGGCCR 2 cut(s) 237, 441
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 246
AcyI GRCGYC 2 cut(s) 145, 216
AfaI GTAC 3 cut(s) 101, 293, 703
AfiI CCNNNNNNNGG 3 cut(s) 279, 497, 626
AgsI TTSAA 1 cut(s) 41
AhlI ACTAGT 1 cut(s) 133
AjiI CACGTC 1 cut(s) 561
AluBI AGCT 4 cut(s) 77, 224, 691, 717
AluI AGCT 4 cut(s) 77, 224, 691, 717
Alw21I GWGCWC 3 cut(s) 566, 693, 719
Alw26I GTCTC 2 cut(s) 61, 152
Alw44I GTGCAC 1 cut(s) 562
AlwI GGATC 5 cut(s) 202, 215, 272, 587, 630
Aor13HI TCCGGA 1 cut(s) 261
AoxI GGCC 2 cut(s) 237, 441
ApaLI GTGCAC 1 cut(s) 562
ApeKI GCWGC 3 cut(s) 461, 550, 616
ApoI RAATTY 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 243, 275
AspA2I CCTAGG 1 cut(s) 184
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 2 cut(s) 7, 484
AsuC2I CCSGG 2 cut(s) 499, 683
AsuHPI GGTGA 2 cut(s) 313, 547
AvaII GGWCC 2 cut(s) 7, 484
AvrII CCTAGG 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 566
BalI TGGCCA 1 cut(s) 239
BamHI GGATCC 1 cut(s) 207
BanII GRGCYC 2 cut(s) 693, 719
BauI CACGAG 1 cut(s) 583
BbsI GAAGAC 2 cut(s) 48, 234
Bbv12I GWGCWC 3 cut(s) 566, 693, 719
BbvI GCAGC 3 cut(s) 448, 562, 603
BccI CCATC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 260
BcnI CCSGG 2 cut(s) 499, 683
BcoDI GTCTC 2 cut(s) 61, 152
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 2 cut(s) 134, 185
BfmI CTRYAG 3 cut(s) 166, 427, 660
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 4 cut(s) 462, 551, 589, 617
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 4 cut(s) 463, 552, 590, 618
Bme1390I CCNGG 2 cut(s) 499, 683
Bme18I GGWCC 2 cut(s) 7, 484
BmgBI CACGTC 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 7, 484
BmiI GGNNCC 2 cut(s) 8, 209
BmrFI CCNGG 2 cut(s) 499, 683
BmsI GCATC 1 cut(s) 516
BpiI GAAGAC 2 cut(s) 48, 234
BpuMI CCSGG 2 cut(s) 499, 683
BsaAI YACGTR 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 506
BsaHI GRCGYC 2 cut(s) 145, 216
BsaJI CCNNGG 2 cut(s) 184, 554
BsaWI WCCGGW 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 279, 497, 626
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 2 cut(s) 177, 477
Bse8I GATNNNNATC 1 cut(s) 506
BseAI TCCGGA 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 184, 554
BseGI GGATG 4 cut(s) 70, 240, 357, 717
BseJI GATNNNNATC 1 cut(s) 506
BseLI CCNNNNNNNGG 3 cut(s) 279, 497, 626
BseMI GCAATG 2 cut(s) 177, 477
BseMII CTCAG 2 cut(s) 399, 624
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 3 cut(s) 300, 328, 588
BseSI GKGCMC 1 cut(s) 566
BseXI GCAGC 3 cut(s) 448, 562, 603
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 2 cut(s) 239, 443
BsiHKAI GWGCWC 3 cut(s) 566, 693, 719
BsiSI CCGG 6 cut(s) 201, 262, 357, 498, 620, 682
BslFI GGGAC 2 cut(s) 107, 446
BslI CCNNNNNNNGG 3 cut(s) 279, 497, 626
BsmAI GTCTC 2 cut(s) 61, 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 107, 446
BsmI GAATGC 1 cut(s) 123
BsnI GGCC 2 cut(s) 239, 443
Bsp1286I GDGCHC 3 cut(s) 566, 693, 719
Bsp13I TCCGGA 1 cut(s) 261
Bsp1407I TGTACA 1 cut(s) 701
Bsp143I GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 6 cut(s) 87, 204, 273, 482, 533, 588
BspANI GGCC 2 cut(s) 239, 443
BspCNI CTCAG 2 cut(s) 400, 625
BspEI TCCGGA 1 cut(s) 261
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 8, 209
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 5 cut(s) 202, 215, 272, 587, 630
BsrBI CCGCTC 2 cut(s) 87, 588
BsrDI GCAATG 2 cut(s) 177, 477
BsrFI RCCGGY 1 cut(s) 356
BsrGI TGTACA 1 cut(s) 701
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 184, 554
BssMI GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
BssNI GRCGYC 2 cut(s) 145, 216
BssSI CACGAG 1 cut(s) 583
BssT1I CCWWGG 2 cut(s) 184, 554
Bst2BI CACGAG 1 cut(s) 583
Bst4CI ACNGT 1 cut(s) 699
BstACI GRCGYC 2 cut(s) 145, 216
BstAUI TGTACA 1 cut(s) 701
BstBAI YACGTR 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 408, 633
BstDSI CCRYGG 1 cut(s) 554
BstF5I GGATG 4 cut(s) 70, 240, 357, 717
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 7 cut(s) 210, 267, 414, 582, 613, 625, 670
BstMAI GTCTC 2 cut(s) 61, 152
BstMBI GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
BstMWI GCNNNNNNNGC 3 cut(s) 179, 539, 638
BstNSI RCATGY 2 cut(s) 156, 377
BstSCI CCNGG 2 cut(s) 497, 681
BstSFI CTRYAG 3 cut(s) 166, 427, 660
BstSLI GKGCMC 1 cut(s) 566
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 3 cut(s) 448, 562, 603
BstV2I GAAGAC 2 cut(s) 48, 234
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 2 cut(s) 239, 443
BtgI CCRYGG 1 cut(s) 554
BtrI CACGTC 1 cut(s) 561
BtsCI GGATG 4 cut(s) 70, 240, 357, 717
BtsI GCAGTG 2 cut(s) 196, 401
BtsIMutI CAGTG 3 cut(s) 196, 401, 704
CfoI GCGC 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 7, 484
Csp6I GTAC 3 cut(s) 100, 292, 702
CviAII CATG 5 cut(s) 153, 325, 374, 436, 555
CviJI RGCY 9 cut(s) 77, 224, 239, 443, 461, 553, 641, 691, 717
CviKI_1 RGCY 9 cut(s) 77, 224, 239, 443, 461, 553, 641, 691, 717
CviQI GTAC 3 cut(s) 100, 292, 702
DdeI CTNAG 2 cut(s) 408, 633
DpnI GATC 7 cut(s) 209, 266, 413, 581, 612, 624, 669
DpnII GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
EaeI YGGCCR 2 cut(s) 237, 441
Ecl136II GAGCTC 2 cut(s) 691, 717
Eco130I CCWWGG 2 cut(s) 184, 554
Eco24I GRGCYC 2 cut(s) 693, 719
Eco47I GGWCC 2 cut(s) 7, 484
Eco53kI GAGCTC 2 cut(s) 691, 717
Eco57I CTGAAG 1 cut(s) 246
EcoICRI GAGCTC 2 cut(s) 691, 717
EcoT14I CCWWGG 2 cut(s) 184, 554
EcoT22I ATGCAT 1 cut(s) 158
EcoT38I GRGCYC 2 cut(s) 693, 719
ErhI CCWWGG 2 cut(s) 184, 554
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 5 cut(s) 156, 328, 377, 439, 558
FaqI GGGAC 2 cut(s) 107, 446
FatI CATG 5 cut(s) 152, 324, 373, 435, 554
FauI CCCGC 1 cut(s) 197
Fnu4HI GCNGC 4 cut(s) 462, 551, 589, 617
FokI GGATG 3 cut(s) 77, 227, 364
FriOI GRGCYC 2 cut(s) 693, 719
Fsp4HI GCNGC 4 cut(s) 462, 551, 589, 617
FspBI CTAG 2 cut(s) 134, 185
GlaI GCGC 1 cut(s) 172
GluI GCNGC 4 cut(s) 462, 551, 589, 617
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 2 cut(s) 239, 443
HapII CCGG 6 cut(s) 201, 262, 357, 498, 620, 682
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 2 cut(s) 145, 216
Hin1II CATG 5 cut(s) 156, 328, 377, 439, 558
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 3 cut(s) 251, 318, 595
HpaII CCGG 6 cut(s) 201, 262, 357, 498, 620, 682
HphI GGTGA 2 cut(s) 313, 547
Hpy166II GTNNAC 3 cut(s) 495, 564, 702
Hpy188I TCNGA 2 cut(s) 143, 221
Hpy188III TCNNGA 4 cut(s) 262, 268, 511, 583
Hpy8I GTNNAC 3 cut(s) 495, 564, 702
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 2 cut(s) 467, 532
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4IV ACGT 4 cut(s) 145, 216, 294, 560
HpyCH4V TGCA 9 cut(s) 24, 110, 156, 328, 401, 464, 507, 564, 662
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 539, 638
HpyF3I CTNAG 2 cut(s) 408, 633
HpySE526I ACGT 4 cut(s) 145, 216, 294, 560
Hsp92I GRCGYC 2 cut(s) 145, 216
Hsp92II CATG 5 cut(s) 156, 328, 377, 439, 558
HspAI GCGC 1 cut(s) 171
Kpn2I TCCGGA 1 cut(s) 261
Kzo9I GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
Lsp1109I GCAGC 3 cut(s) 448, 562, 603
LweI GCATC 1 cut(s) 516
MaeI CTAG 2 cut(s) 134, 185
MaeII ACGT 4 cut(s) 145, 216, 294, 560
MaeIII GTNAC 4 cut(s) 369, 451, 517, 592
MalI GATC 7 cut(s) 209, 266, 413, 581, 612, 624, 669
MbiI CCGCTC 2 cut(s) 87, 588
MboI GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
MboII GAAGA 3 cut(s) 53, 239, 556
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 3 cut(s) 566, 693, 719
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 5 cut(s) 11, 16, 36, 105, 361
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 2 cut(s) 166, 450
MnlI CCTC 5 cut(s) 278, 281, 306, 568, 609
Mox20I TGGCCA 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 158
MroI TCCGGA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 239
MseI TTAA 3 cut(s) 14, 81, 423
MslI CAYNNNNRTG 2 cut(s) 378, 559
Msp20I TGGCCA 1 cut(s) 239
MspI CCGG 6 cut(s) 201, 262, 357, 498, 620, 682
MspR9I CCNGG 2 cut(s) 499, 683
Mva1269I GAATGC 1 cut(s) 123
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 3 cut(s) 179, 539, 638
NciI CCSGG 2 cut(s) 499, 683
NcoI CCATGG 1 cut(s) 554
NdeII GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
NlaIII CATG 5 cut(s) 156, 328, 377, 439, 558
NlaIV GGNNCC 2 cut(s) 8, 209
NmuCI GTSAC 4 cut(s) 369, 451, 517, 592
NsiI ATGCAT 1 cut(s) 158
NspI RCATGY 2 cut(s) 156, 377
PcsI WCGNNNNNNNCGW 1 cut(s) 518
PctI GAATGC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 251, 318
PkrI GCNGC 4 cut(s) 463, 552, 590, 618
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 295
Psp124BI GAGCTC 2 cut(s) 693, 719
PspN4I GGNNCC 2 cut(s) 8, 209
PspPI GGNCC 2 cut(s) 7, 484
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 1 cut(s) 207
RsaI GTAC 3 cut(s) 101, 293, 703
RsaNI GTAC 3 cut(s) 100, 292, 702
RseI CAYNNNNRTG 2 cut(s) 378, 559
SacI GAGCTC 2 cut(s) 693, 719
SaqAI TTAA 3 cut(s) 14, 81, 423
SatI GCNGC 4 cut(s) 462, 551, 589, 617
Sau3AI GATC 7 cut(s) 207, 264, 411, 579, 610, 622, 667
Sau96I GGNCC 2 cut(s) 7, 484
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 2 cut(s) 499, 683
SduI GDGCHC 3 cut(s) 566, 693, 719
SfaNI GCATC 1 cut(s) 516
SfcI CTRYAG 3 cut(s) 166, 427, 660
SinI GGWCC 2 cut(s) 7, 484
SmiMI CAYNNNNRTG 2 cut(s) 378, 559
SpeI ACTAGT 1 cut(s) 133
Sse9I AATT 5 cut(s) 11, 16, 36, 105, 361
SsiI CCGC 6 cut(s) 87, 204, 273, 482, 533, 588
SspI AATATT 1 cut(s) 421
SspMI CTAG 2 cut(s) 134, 185
SstI GAGCTC 2 cut(s) 693, 719
StyD4I CCNGG 2 cut(s) 497, 681
StyI CCWWGG 2 cut(s) 184, 554
TaaI ACNGT 1 cut(s) 699
TaiI ACGT 4 cut(s) 148, 219, 297, 563
TaqI TCGA 1 cut(s) 512
TasI AATT 5 cut(s) 11, 16, 36, 105, 361
TatI WGTACW 1 cut(s) 701
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 2 cut(s) 251, 318
Tru1I TTAA 3 cut(s) 14, 81, 423
Tru9I TTAA 3 cut(s) 14, 81, 423
TscAI CASTG 3 cut(s) 196, 408, 704
TseFI GTSAC 4 cut(s) 369, 451, 517, 592
TseI GCWGC 3 cut(s) 461, 550, 616
Tsp45I GTSAC 4 cut(s) 369, 451, 517, 592
TspDTI ATGAA 1 cut(s) 350
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 3 cut(s) 196, 408, 704
VneI GTGCAC 1 cut(s) 562
VpaK11BI GGWCC 2 cut(s) 7, 484
XapI RAATTY 1 cut(s) 16
XceI RCATGY 2 cut(s) 156, 377
XmaJI CCTAGG 1 cut(s) 184
XspI CTAG 2 cut(s) 134, 185
ZraI GACGTC 2 cut(s) 146, 217
Zsp2I ATGCAT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.