Rw5G013160

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005230
Physical Location & Seq
Reverse (-)
15491160 .. 15496298
5139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013160.1

Sequence Viewer

Length: 1359 bp
ATGGAACATTGGATATTCACAACGCCGATTACGACGACTTCCGAACTACATGTGATGAGATCACTACTGTTAACACATGTTGTGGGCTGCTTACTCCTTATTGGAGGGGGTGACAGCAGAAAAGTATTCAAGCTCTCCGAGGAGAAATCCGAATCTGAATCCACCTTCGAGTACAATGCCATCAGCTGCAGAGCCCATAGCGCTGCGCTCACTGATTTCGGAGGAGTAGGAGACGGCAAGACATCAAACACAAAGGCCTTTGAAGCTGCAATCAACCACCTGAGTCACTTCAACCTCACCAGCCACTTCACTCTTTACCTCCACAAAGAGGTTGTTCTCCTCGCTTCTCAAGACCCCCGAGAATGGCCGGTGCTCAAACCACTCCCGTCTTACGGCAGAGGAAGGGACGCACCAGGTGGACGCTACGCCAGCCTCATATTCTGCACCAACCTCACCGACGTCGTCATCACCGGAGACAACGGCACAATCGACGGTCAAGGTGAATTCTGGTGGAACAAATTCCACAGTAAAAAGTTATACAGCAATATACTAGTGCAAGGATTGACCATCATAGCTCCGGTGAGGTCTCCGAACACAGATGGAATTAATCCGGATTCATGCACAAACGTCAAGATTGAGGACTGTTACATTATCTCCGGCGACGACTGCGTGGCCGTCAAGAGCGGTTGGGACCAGTACGGCGTGTCGTTGGGTATGCCAACCAAGCAACTAGTGATCAGACTACTTACTTGTGTTTCGCCTTACAGCGCCGCCATTGCATTGGACAGCGAGATGTCCGGCGGGATTGAAGACGTCAGAGCAGAAGACATCGTGGCCGTTGATACGGAATCCGGGGTGAGGATCAAGACAGCTATAGGGCAAGGAGGGTACGTGAGGGACGTGTGCTTAAGGAGAATGGAAATGCACACCATGATGTGGGCGTTTAGGATGAGTGGCGATTATGGGTCACACGCTGACGATGGCTATGACAAGATGGCAATTCCTGAGATCAAAGGGATTCACTACAGGGATGTGGTGGCTGATAATGTGACGATGGCGGCGAAACTGGAGGGGATTGCTGGCCATCCGTTTACGGGGATTTGCATGTCGAATGTGACAGTTGGATTGGCTGCCAAGGCAAAGAAGCAGCCGTGGAGCTGTGTTGATATTGAGGGGGTTGCAAGCGGCGTGACGCCGACACCGTGTGATTTGCTACACGGCGATGATCCGAGTCAGGGTGATGTTGCTAGTGGTGGCTGTGATTTTCGCCTCTTTCATCTCCTAGTCGTTCTAGGAGTCCAAGTTCGTCTGATCAGGACCCTGCGGACAAGGATCCAGCTCCGAGTCCATCATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

452

Amino Acids

49.33

Weight (kDa)

6.05

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 181 - 390 7.6e-22 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 462, 816
AccB7I CCANNNNNTGG 1 cut(s) 936
AccBSI CCGCTC 1 cut(s) 684
AccIII TCCGGA 1 cut(s) 610
AciI CCGC 6 cut(s) 684, 771, 801, 1058, 1185, 1324
AclWI GGATC 4 cut(s) 869, 1220, 1327, 1340
AcoI YGGCCR 4 cut(s) 365, 672, 834, 1081
AcsI RAATTY 2 cut(s) 503, 518
AcyI GRCGYC 3 cut(s) 459, 813, 1193
AdeI CACNNNGTG 2 cut(s) 416, 1205
AfaI GTAC 3 cut(s) 173, 698, 890
AfeI AGCGCT 1 cut(s) 202
AfiI CCNNNNNNNGG 7 cut(s) 328, 363, 392, 858, 936, 1094, 1235
AflII CTTAAG 1 cut(s) 907
AflIII ACRYGT 3 cut(s) 49, 76, 900
AgsI TTSAA 4 cut(s) 130, 263, 292, 809
AhlI ACTAGT 2 cut(s) 550, 730
AjiI CACGTC 1 cut(s) 901
AjnI CCWGG 1 cut(s) 412
AloI GAACNNNNNNTCC 2 cut(s) 1287, 1319
AluBI AGCT 7 cut(s) 133, 186, 266, 575, 872, 1158, 1339
AluI AGCT 7 cut(s) 133, 186, 266, 575, 872, 1158, 1339
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 3 cut(s) 225, 468, 591
AlwI GGATC 4 cut(s) 869, 1220, 1327, 1340
Ama87I CYCGRG 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 610
Aor51HI AGCGCT 1 cut(s) 202
AoxI GGCC 5 cut(s) 255, 365, 672, 834, 1081
ApeKI GCWGC 6 cut(s) 87, 186, 203, 266, 1130, 1147
ApoI RAATTY 2 cut(s) 503, 518
AseI ATTAAT 1 cut(s) 606
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 3 cut(s) 203, 208, 770
AspS9I GGNCC 2 cut(s) 691, 1317
AsuC2I CCSGG 1 cut(s) 853
AsuHPI GGTGA 8 cut(s) 122, 289, 445, 460, 512, 592, 868, 1250
AvaI CYCGRG 1 cut(s) 357
AvaII GGWCC 2 cut(s) 691, 1317
BalI TGGCCA 1 cut(s) 1083
BamHI GGATCC 1 cut(s) 1332
BanII GRGCYC 1 cut(s) 196
BbsI GAAGAC 2 cut(s) 816, 831
Bbv12I GWGCWC 1 cut(s) 375
BbvI GCAGC 6 cut(s) 74, 173, 190, 253, 1117, 1159
BccI CCATC 8 cut(s) 188, 575, 593, 974, 988, 1048, 1092, 1356
BceAI ACGGC 8 cut(s) 250, 409, 496, 659, 715, 821, 1135, 1234
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 2 cut(s) 735, 1311
BcnI CCSGG 1 cut(s) 853
BcoDI GTCTC 3 cut(s) 225, 468, 591
BcuI ACTAGT 2 cut(s) 550, 730
BfaI CTAG 5 cut(s) 551, 731, 1248, 1283, 1292
BfmI CTRYAG 3 cut(s) 187, 873, 1024
BfoI RGCGCY 2 cut(s) 204, 771
BfrI CTTAAG 1 cut(s) 907
BisI GCNGC 9 cut(s) 88, 187, 204, 267, 771, 1059, 1131, 1148, 1186
BlsI GCNGC 9 cut(s) 89, 188, 205, 268, 772, 1060, 1132, 1149, 1187
Bme1390I CCNGG 2 cut(s) 414, 853
Bme18I GGWCC 2 cut(s) 691, 1317
BmeT110I CYCGRG 1 cut(s) 357
BmgBI CACGTC 1 cut(s) 901
BmgT120I GGNCC 2 cut(s) 691, 1317
BmiI GGNNCC 3 cut(s) 692, 1319, 1334
BmrFI CCNGG 2 cut(s) 414, 853
BpiI GAAGAC 2 cut(s) 816, 831
BpmI CTGGAG 1 cut(s) 1088
BpuEI CTTGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 853
BsaAI YACGTR 1 cut(s) 892
BsaHI GRCGYC 3 cut(s) 459, 813, 1193
BsaI GGTCTC 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 138, 852, 1134, 1151
BsaWI WCCGGW 3 cut(s) 470, 577, 610
BsaXI ACNNNNNCTCC 6 cut(s) 638, 668, 876, 906, 1287, 1317
Bsc4I CCNNNNNNNGG 7 cut(s) 328, 363, 392, 858, 936, 1094, 1235
Bse118I RCCGGY 1 cut(s) 367
Bse1I ACTGG 2 cut(s) 694, 1071
Bse3DI GCAATG 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 4 cut(s) 138, 852, 1134, 1151
BseGI GGATG 3 cut(s) 954, 1036, 1084
BseLI CCNNNNNNNGG 7 cut(s) 328, 363, 392, 858, 936, 1094, 1235
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 272, 996
BseNI ACTGG 2 cut(s) 694, 1071
BseRI GAGGAG 3 cut(s) 155, 237, 329
BseXI GCAGC 6 cut(s) 74, 173, 190, 253, 1117, 1159
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 5 cut(s) 257, 367, 674, 836, 1083
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 7 cut(s) 368, 471, 578, 611, 657, 798, 852
BslFI GGGAC 3 cut(s) 419, 704, 911
BslI CCNNNNNNNGG 7 cut(s) 328, 363, 392, 858, 936, 1094, 1235
BsmAI GTCTC 3 cut(s) 225, 468, 591
BsmBI CGTCTC 1 cut(s) 225
BsmFI GGGAC 3 cut(s) 419, 704, 911
BsnI GGCC 5 cut(s) 257, 367, 674, 836, 1083
Bso31I GGTCTC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 357
Bsp1286I GDGCHC 2 cut(s) 196, 375
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
BspACI CCGC 6 cut(s) 684, 771, 801, 1058, 1185, 1324
BspANI GGCC 5 cut(s) 257, 367, 674, 836, 1083
BspCNI CTCAG 2 cut(s) 273, 997
BspEI TCCGGA 1 cut(s) 610
BspLI GGNNCC 3 cut(s) 692, 1319, 1334
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 4 cut(s) 869, 1220, 1327, 1340
BspTI CTTAAG 1 cut(s) 907
BspTNI GGTCTC 1 cut(s) 591
BsrBI CCGCTC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 367
BsrI ACTGG 2 cut(s) 694, 1071
BssAI RCCGGY 1 cut(s) 367
BssECI CCNNGG 4 cut(s) 138, 852, 1134, 1151
BssMI GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
BssNI GRCGYC 3 cut(s) 459, 813, 1193
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 6 cut(s) 69, 494, 527, 644, 1120, 1203
BstACI GRCGYC 3 cut(s) 459, 813, 1193
BstAFI CTTAAG 1 cut(s) 907
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 3 cut(s) 430, 1081, 1183
BstDEI CTNAG 2 cut(s) 281, 1005
BstDSI CCRYGG 1 cut(s) 1151
BstF5I GGATG 3 cut(s) 954, 1036, 1084
BstH2I RGCGCY 2 cut(s) 204, 771
BstHHI GCGC 3 cut(s) 203, 208, 770
BstKTI GATC 7 cut(s) 62, 738, 864, 1011, 1228, 1314, 1335
BstMAI GTCTC 3 cut(s) 225, 468, 591
BstMBI GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
BstMWI GCNNNNNNNGC 7 cut(s) 200, 263, 429, 666, 724, 776, 1136
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 3 cut(s) 53, 80, 1108
BstSCI CCNGG 2 cut(s) 412, 851
BstSFI CTRYAG 3 cut(s) 187, 873, 1024
BstV1I GCAGC 6 cut(s) 74, 173, 190, 253, 1117, 1159
BstV2I GAAGAC 2 cut(s) 816, 831
BstX2I RGATCY 1 cut(s) 1332
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 1 cut(s) 1332
BsuRI GGCC 5 cut(s) 257, 367, 674, 836, 1083
BtgI CCRYGG 1 cut(s) 1151
BtgZI GCGATG 1 cut(s) 1236
BtrI CACGTC 1 cut(s) 901
BtsCI GGATG 3 cut(s) 954, 1036, 1084
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 430, 1081, 1183
CfoI GCGC 3 cut(s) 203, 208, 770
Cfr10I RCCGGY 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 691, 1317
CseI GACGC 3 cut(s) 416, 429, 1201
CsiI ACCWGGT 1 cut(s) 412
Csp6I GTAC 3 cut(s) 172, 697, 889
CviAII CATG 5 cut(s) 50, 77, 618, 931, 1105
CviQI GTAC 3 cut(s) 172, 697, 889
DdeI CTNAG 2 cut(s) 281, 1005
DpnI GATC 7 cut(s) 61, 737, 863, 1010, 1227, 1313, 1334
DpnII GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
DraIII CACNNNGTG 2 cut(s) 416, 1205
EaeI YGGCCR 4 cut(s) 365, 672, 834, 1081
Eco130I CCWWGG 1 cut(s) 1134
Eco147I AGGCCT 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 591
Eco47I GGWCC 2 cut(s) 691, 1317
Eco47III AGCGCT 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 1317
EcoRI GAATTC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 1134
EcoT38I GRGCYC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 1134
Esp3I CGTCTC 1 cut(s) 225
FaeI CATG 5 cut(s) 53, 80, 621, 934, 1108
FaqI GGGAC 3 cut(s) 419, 704, 911
FatI CATG 5 cut(s) 49, 76, 617, 930, 1104
FauI CCCGC 1 cut(s) 794
FbaI TGATCA 2 cut(s) 735, 1311
Fnu4HI GCNGC 9 cut(s) 88, 187, 204, 267, 771, 1059, 1131, 1148, 1186
FokI GGATG 3 cut(s) 961, 1043, 1071
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 9 cut(s) 88, 187, 204, 267, 771, 1059, 1131, 1148, 1186
FspBI CTAG 5 cut(s) 551, 731, 1248, 1283, 1292
GlaI GCGC 3 cut(s) 202, 207, 769
GluI GCNGC 9 cut(s) 88, 187, 204, 267, 771, 1059, 1131, 1148, 1186
GsuI CTGGAG 1 cut(s) 1088
HaeII RGCGCY 2 cut(s) 204, 771
HaeIII GGCC 5 cut(s) 257, 367, 674, 836, 1083
HapII CCGG 7 cut(s) 368, 471, 578, 611, 657, 798, 852
HgaI GACGC 3 cut(s) 416, 429, 1201
HhaI GCGC 3 cut(s) 203, 208, 770
Hin1I GRCGYC 3 cut(s) 459, 813, 1193
Hin1II CATG 5 cut(s) 53, 80, 621, 934, 1108
Hin6I GCGC 3 cut(s) 201, 206, 768
HinP1I GCGC 3 cut(s) 201, 206, 768
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 9 cut(s) 152, 158, 283, 614, 848, 1018, 1231, 1296, 1344
HpaI GTTAAC 1 cut(s) 72
HpaII CCGG 7 cut(s) 368, 471, 578, 611, 657, 798, 852
HphI GGTGA 8 cut(s) 122, 289, 445, 460, 512, 592, 868, 1250
Hpy166II GTNNAC 3 cut(s) 72, 419, 1092
Hpy188III TCNNGA 7 cut(s) 350, 611, 631, 679, 865, 1004, 1315
Hpy8I GTNNAC 3 cut(s) 72, 419, 1092
Hpy99I CGWCG 5 cut(s) 37, 461, 464, 494, 665
HpyAV CCTTC 2 cut(s) 175, 396
HpyCH4III ACNGT 6 cut(s) 69, 494, 527, 644, 1120, 1203
HpyCH4IV ACGT 5 cut(s) 459, 627, 813, 891, 900
HpyCH4V TGCA 9 cut(s) 189, 269, 444, 556, 621, 779, 925, 1104, 1181
HpyF10VI GCNNNNNNNGC 7 cut(s) 200, 263, 429, 666, 724, 776, 1136
HpyF3I CTNAG 2 cut(s) 281, 1005
HpySE526I ACGT 5 cut(s) 459, 627, 813, 891, 900
Hsp92I GRCGYC 3 cut(s) 459, 813, 1193
Hsp92II CATG 5 cut(s) 53, 80, 621, 934, 1108
HspAI GCGC 3 cut(s) 201, 206, 768
Kpn2I TCCGGA 1 cut(s) 610
Ksp22I TGATCA 2 cut(s) 735, 1311
KspAI GTTAAC 1 cut(s) 72
Kzo9I GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
LmnI GCTCC 3 cut(s) 580, 1155, 1344
Lsp1109I GCAGC 6 cut(s) 74, 173, 190, 253, 1117, 1159
MabI ACCWGGT 1 cut(s) 412
MaeI CTAG 5 cut(s) 551, 731, 1248, 1283, 1292
MaeII ACGT 5 cut(s) 459, 627, 813, 891, 900
MaeIII GTNAC 7 cut(s) 110, 284, 644, 966, 1048, 1114, 1189
MalI GATC 7 cut(s) 61, 737, 863, 1010, 1227, 1313, 1334
MbiI CCGCTC 1 cut(s) 684
MboI GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
MboII GAAGA 2 cut(s) 821, 836
MflI RGATCY 1 cut(s) 1332
MhlI GDGCHC 2 cut(s) 196, 375
MlsI TGGCCA 1 cut(s) 1083
MluCI AATT 4 cut(s) 503, 518, 603, 999
MluNI TGGCCA 1 cut(s) 1083
MlyI GAGTC 4 cut(s) 292, 1240, 1305, 1353
MmeI TCCRAC 1 cut(s) 1102
Mox20I TGGCCA 1 cut(s) 1083
MroI TCCGGA 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 518
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 3 cut(s) 71, 606, 908
MslI CAYNNNNRTG 2 cut(s) 932, 1221
Msp20I TGGCCA 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 186
MspCI CTTAAG 1 cut(s) 907
MspI CCGG 7 cut(s) 368, 471, 578, 611, 657, 798, 852
MspR9I CCNGG 2 cut(s) 414, 853
MteI GCGCNGCGC 1 cut(s) 204
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 7 cut(s) 200, 263, 429, 666, 724, 776, 1136
NciI CCSGG 1 cut(s) 853
NdeII GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
NlaIII CATG 5 cut(s) 53, 80, 621, 934, 1108
NlaIV GGNNCC 3 cut(s) 692, 1319, 1334
NmuCI GTSAC 6 cut(s) 110, 284, 966, 1048, 1114, 1189
NspI RCATGY 3 cut(s) 53, 80, 1108
PceI AGGCCT 1 cut(s) 257
PciI ACATGT 2 cut(s) 49, 76
PcsI WCGNNNNNNNCGW 5 cut(s) 29, 486, 897, 1058, 1199
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 5 cut(s) 152, 158, 614, 848, 1018
PflFI GACNNNGTC 1 cut(s) 461
PflMI CCANNNNNTGG 1 cut(s) 936
PkrI GCNGC 9 cut(s) 89, 188, 205, 268, 772, 1060, 1132, 1149, 1187
PleI GAGTC 4 cut(s) 291, 1239, 1304, 1352
PpsI GAGTC 4 cut(s) 291, 1239, 1304, 1352
Ppu21I YACGTR 1 cut(s) 892
PpuMI RGGWCCY 1 cut(s) 1317
PscI ACATGT 2 cut(s) 49, 76
PshBI ATTAAT 1 cut(s) 606
Psp5II RGGWCCY 1 cut(s) 1317
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 3 cut(s) 692, 1319, 1334
PspPI GGNCC 2 cut(s) 691, 1317
PspPPI RGGWCCY 1 cut(s) 1317
PstI CTGCAG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 1332
PsyI GACNNNGTC 1 cut(s) 461
PvuII CAGCTG 1 cut(s) 186
RsaI GTAC 3 cut(s) 173, 698, 890
RsaNI GTAC 3 cut(s) 172, 697, 889
RseI CAYNNNNRTG 2 cut(s) 932, 1221
SaqAI TTAA 3 cut(s) 71, 606, 908
SatI GCNGC 9 cut(s) 88, 187, 204, 267, 771, 1059, 1131, 1148, 1186
Sau3AI GATC 7 cut(s) 59, 735, 861, 1008, 1225, 1311, 1332
Sau96I GGNCC 2 cut(s) 691, 1317
SchI GAGTC 4 cut(s) 292, 1240, 1305, 1353
ScrFI CCNGG 2 cut(s) 414, 853
SduI GDGCHC 2 cut(s) 196, 375
SexAI ACCWGGT 1 cut(s) 412
SfcI CTRYAG 3 cut(s) 187, 873, 1024
SinI GGWCC 2 cut(s) 691, 1317
SmiMI CAYNNNNRTG 2 cut(s) 932, 1221
SmlI CTYRAG 2 cut(s) 348, 907
SmoI CTYRAG 2 cut(s) 348, 907
SpeI ACTAGT 2 cut(s) 550, 730
Sse9I AATT 4 cut(s) 503, 518, 603, 999
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 6 cut(s) 684, 771, 801, 1058, 1185, 1324
SspMI CTAG 5 cut(s) 551, 731, 1248, 1283, 1292
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 412, 851
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 6 cut(s) 69, 494, 527, 644, 1120, 1203
TaiI ACGT 5 cut(s) 462, 630, 816, 894, 903
TaqI TCGA 3 cut(s) 168, 489, 1109
TasI AATT 4 cut(s) 503, 518, 603, 999
TatI WGTACW 1 cut(s) 171
TauI GCSGC 3 cut(s) 773, 1061, 1188
TfiI GAWTC 5 cut(s) 152, 158, 614, 848, 1018
Tru1I TTAA 3 cut(s) 71, 606, 908
Tru9I TTAA 3 cut(s) 71, 606, 908
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 6 cut(s) 110, 284, 966, 1048, 1114, 1189
TseI GCWGC 6 cut(s) 87, 186, 203, 266, 1130, 1147
Tsp45I GTSAC 6 cut(s) 110, 284, 966, 1048, 1114, 1189
TspDTI ATGAA 2 cut(s) 606, 1265
TspGWI ACGGA 2 cut(s) 860, 1077
TspRI CASTG 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 461
Van91I CCANNNNNTGG 1 cut(s) 936
Vha464I CTTAAG 1 cut(s) 907
VpaK11BI GGWCC 2 cut(s) 691, 1317
VspI ATTAAT 1 cut(s) 606
XapI RAATTY 2 cut(s) 503, 518
XceI RCATGY 3 cut(s) 53, 80, 1108
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 5 cut(s) 551, 731, 1248, 1283, 1292
ZraI GACGTC 2 cut(s) 460, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.