AT3G61490

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005230
Physical Location & Seq
Forward (+)
22757761 .. 22760470
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61490.5

Sequence Viewer

Length: 1431 bp
ATGAAGAACATACTAGCTTGCATAGTTACCATCATAACCCTCTCAAACCTCATAACCATTTCTCAAGGAAGAAGGGTATCACAGTCATTTGAAACATTTGAGTACACAGCAATTATTTGCAGATCCCACAGTGCCTCCATAACAGAGTACGGTGGTGTAGGAGACGGCAAGACGCTAAACACAAAGGCCTTCCAGAGCGCCGTTGATCATCTCAGCCAATACTCATCTGAAGGCGGAGCACAGCTCTTTGTACCCGCCGGGAAATGGCTCACCGGAAGTTTCAACCTCACAAGCCATTTCACTCTGTTTCTTCACAAAGACGCAATTCTTCTCGCTGCTCAAGACTTAAACGAATACCCAATTCTAAAGGCTTTGCCTTCGTACGGACGAGGACGTGACGCCGCCGGTGGAAGATTCGCTAGTCTCATCTTCGGCACTAATCTCTCCGACGTAATTATCACCGGGAACAACGGCACAATCGACGGTCAAGGATCGTTTTGGTGGCAAAAGTTCCACGGTGGCAAGCTAAAATACACTCGCCCGTACCTGATTGAGTTAATGTTCTCAGATACAATTCAGATTTCAAATCTAACGTTCCTTGATTCTCCATCGTGGAACATCCACCCAGTGTATAGTAGCAATATCATCGTAAAAGGCGTTACGATCATCGCCCCGGTAAAATCTCCGAACACCGACGGAATCAATCCAGATTCTTGCACGAACACGAGGATCGAAGACTGTTACATCATATCCGGCGACGATTGCATCGCCGTGAAAAGCGGATGGGACGAATACGGAATCTCATTCGGAATGCCGACGAAGCACCTCGTGATCCGTAGATTAACCTGTATCTCACCGTACAGCGCCGCGATCGCTCTCGGAAGCGAAATGTCCGGCGGAATCGAAGACGTGAGAGCGGAAGATATCACCGCATACCAAACAGAATCCGGCGTACGGATAAAAACCGCCGTAGGAAGAGGAGCTTTCGTGAAGAACATATACGTAAAAGGAATGAATCTACACACGATGAAATGGGTTTTTTGGATGACCGGAAATTACAAAGCTCATGCTGATTCTCATTACGATCCTCACGCTCTACCGGAGATAACCGGGATTAATTACAGAGATATTGTAGCTGAGAATGTTTCTATGGCTGGAAGGTTAGAGGGAATCTCTGGAGATCCTTTTACTGGGATTTGTATTTCGAATGCAACGATTTCTATGGCGGCTAAGCATAAGAAAGCGATTTGGATGTGTAGTGATGTTGAAGGAGTTACGAGTGGTGTTGATCCTAAGCCTTGTGATTTGCTTGATGGACAAGAATCAGAGACGACGAAGAAGAAGATGATTGACGGTGGATGTGATTTTCCTACTGATGTGTTGGAGATTGATAATGTTGAGCTTAAGACTTGTAGTTATCAAATGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

476

Amino Acids

51.94

Weight (kDa)

5.85

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 156 - 438 4.4e-34 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 917
AccII CGCG 1 cut(s) 869
AclI AACGTT 1 cut(s) 593
AclWI GGATC 7 cut(s) 117, 499, 737, 826, 1079, 1175, 1283
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 399
AdeI CACNNNGTG 2 cut(s) 628, 829
AfaI GTAC 7 cut(s) 104, 149, 252, 383, 545, 860, 954
AfiI CCNNNNNNNGG 4 cut(s) 264, 383, 954, 1190
AflII CTTAAG 1 cut(s) 1403
AgsI TTSAA 4 cut(s) 92, 283, 585, 1268
AjiI CACGTC 2 cut(s) 395, 910
AluBI AGCT 7 cut(s) 17, 244, 526, 983, 1064, 1136, 1402
AluI AGCT 7 cut(s) 17, 244, 526, 983, 1064, 1136, 1402
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 3 cut(s) 156, 428, 1322
AlwI GGATC 7 cut(s) 117, 499, 737, 826, 1079, 1175, 1283
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 335
AseI ATTAAT 1 cut(s) 1116
AsiSI GCGATCGC 1 cut(s) 873
AspLEI GCGC 2 cut(s) 200, 866
AsuC2I CCSGG 4 cut(s) 259, 463, 674, 1111
AsuHPI GGTGA 4 cut(s) 262, 451, 846, 919
AsuII TTCGAA 1 cut(s) 1205
BarI GAAGNNNNNNTAC 2 cut(s) 983, 1015
BauI CACGAG 2 cut(s) 724, 827
BbsI GAAGAC 2 cut(s) 741, 912
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 322
BccI CCATC 4 cut(s) 38, 616, 777, 1307
BceAI ACGGC 5 cut(s) 181, 185, 487, 755, 953
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 4 cut(s) 259, 463, 674, 1111
BcoDI GTCTC 3 cut(s) 156, 428, 1322
BfaI CTAG 2 cut(s) 14, 420
BfoI RGCGCY 2 cut(s) 201, 867
BfrI CTTAAG 1 cut(s) 1403
BisI GCNGC 4 cut(s) 336, 402, 867, 1227
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 4 cut(s) 337, 403, 868, 1228
Bme1390I CCNGG 4 cut(s) 259, 463, 674, 1111
BmgBI CACGTC 2 cut(s) 395, 910
BmrFI CCNGG 4 cut(s) 259, 463, 674, 1111
BmrI ACTGGG 2 cut(s) 620, 1200
BmsI GCATC 1 cut(s) 774
BmuI ACTGGG 2 cut(s) 620, 1200
BpiI GAAGAC 2 cut(s) 741, 912
BplI GAGNNNNNCTC 4 cut(s) 228, 260, 1157, 1189
BpmI CTGGAG 1 cut(s) 1197
Bpu10I CCTNAGC 1 cut(s) 1293
Bpu1102I GCTNAGC 1 cut(s) 1230
Bpu14I TTCGAA 1 cut(s) 1205
BpuEI CTTGAG 2 cut(s) 48, 324
BpuMI CCSGG 4 cut(s) 259, 463, 674, 1111
BsaAI YACGTR 1 cut(s) 1003
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 514, 672
BsaWI WCCGGW 3 cut(s) 272, 1049, 1099
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 972, 1002
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 383, 954, 1190
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 2 cut(s) 626, 1195
BseDI CCNNGG 2 cut(s) 514, 672
BseGI GGATG 5 cut(s) 618, 788, 1050, 1257, 1364
BseLI CCNNNNNNNGG 4 cut(s) 264, 383, 954, 1190
BseMII CTCAG 3 cut(s) 226, 579, 1128
BseNI ACTGG 2 cut(s) 626, 1195
BseRI GAGGAG 1 cut(s) 993
BseXI GCAGC 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 869
Bsh1285I CGRYCG 1 cut(s) 873
BshFI GGCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 241
BsiWI CGTACG 2 cut(s) 381, 952
BslFI GGGAC 1 cut(s) 800
BslI CCNNNNNNNGG 4 cut(s) 264, 383, 954, 1190
BsmAI GTCTC 3 cut(s) 156, 428, 1322
BsmBI CGTCTC 2 cut(s) 156, 1322
BsmFI GGGAC 1 cut(s) 800
BsmI GAATGC 2 cut(s) 816, 1213
BsnI GGCC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 1205
Bsp1286I GDGCHC 1 cut(s) 241
Bsp1720I GCTNAGC 1 cut(s) 1230
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 3 cut(s) 225, 578, 1129
BspFNI CGCG 1 cut(s) 869
BspPI GGATC 7 cut(s) 117, 499, 737, 826, 1079, 1175, 1283
BspT104I TTCGAA 1 cut(s) 1205
BspTI CTTAAG 1 cut(s) 1403
BsrBI CCGCTC 1 cut(s) 917
BsrFI RCCGGY 1 cut(s) 404
BsrI ACTGG 2 cut(s) 626, 1195
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 514, 672
BssNI GRCGYC 1 cut(s) 399
BssSI CACGAG 2 cut(s) 724, 827
Bst2BI CACGAG 2 cut(s) 724, 827
Bst4CI ACNGT 8 cut(s) 84, 131, 152, 485, 518, 740, 858, 1355
Bst6I CTCTTC 1 cut(s) 970
BstACI GRCGYC 1 cut(s) 399
BstAFI CTTAAG 1 cut(s) 1403
BstBAI YACGTR 1 cut(s) 1003
BstBI TTCGAA 1 cut(s) 1205
BstC8I GCNNGC 2 cut(s) 19, 524
BstDEI CTNAG 5 cut(s) 212, 565, 1137, 1230, 1293
BstDSI CCRYGG 1 cut(s) 514
BstF5I GGATG 5 cut(s) 618, 788, 1050, 1257, 1364
BstFNI CGCG 1 cut(s) 869
BstH2I RGCGCY 2 cut(s) 201, 867
BstHHI GCGC 2 cut(s) 200, 866
BstMAI GTCTC 3 cut(s) 156, 428, 1322
BstMCI CGRYCG 1 cut(s) 873
BstMWI GCNNNNNNNGC 3 cut(s) 762, 820, 872
BstSCI CCNGG 4 cut(s) 257, 461, 672, 1109
BstSNI TACGTA 1 cut(s) 1003
BstUI CGCG 1 cut(s) 869
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 2 cut(s) 741, 912
BstX2I RGATCY 2 cut(s) 122, 1180
BstYI RGATCY 2 cut(s) 122, 1180
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 514
BtgZI GCGATG 2 cut(s) 652, 751
BtrI CACGTC 2 cut(s) 395, 910
BtsCI GGATG 5 cut(s) 618, 788, 1050, 1257, 1364
BtsIMutI CAGTG 2 cut(s) 136, 633
Cac8I GCNNGC 2 cut(s) 19, 524
CfoI GCGC 2 cut(s) 200, 866
Cfr10I RCCGGY 1 cut(s) 404
CseI GACGC 3 cut(s) 181, 329, 407
Csp6I GTAC 7 cut(s) 103, 148, 251, 382, 544, 859, 953
CviAII CATG 1 cut(s) 1067
CviQI GTAC 7 cut(s) 103, 148, 251, 382, 544, 859, 953
DdeI CTNAG 5 cut(s) 212, 565, 1137, 1230, 1293
DraIII CACNNNGTG 2 cut(s) 628, 829
Eam1104I CTCTTC 1 cut(s) 970
EarI CTCTTC 1 cut(s) 970
EciI GGCGGA 2 cut(s) 249, 912
Eco105I TACGTA 1 cut(s) 1003
Eco147I AGGCCT 1 cut(s) 188
Eco32I GATATC 1 cut(s) 925
Eco57I CTGAAG 1 cut(s) 249
EcoRV GATATC 1 cut(s) 925
Esp3I CGTCTC 2 cut(s) 156, 1322
FaeI CATG 1 cut(s) 1070
FalI AAGNNNNNCTT 2 cut(s) 967, 999
FaqI GGGAC 1 cut(s) 800
FatI CATG 1 cut(s) 1066
FauI CCCGC 1 cut(s) 262
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 4 cut(s) 336, 402, 867, 1227
FokI GGATG 5 cut(s) 605, 795, 1057, 1264, 1371
Fsp4HI GCNGC 4 cut(s) 336, 402, 867, 1227
FspBI CTAG 2 cut(s) 14, 420
GlaI GCGC 2 cut(s) 199, 865
GluI GCNGC 4 cut(s) 336, 402, 867, 1227
GsuI CTGGAG 1 cut(s) 1197
HaeII RGCGCY 2 cut(s) 201, 867
HaeIII GGCC 1 cut(s) 188
HgaI GACGC 3 cut(s) 181, 329, 407
HhaI GCGC 2 cut(s) 200, 866
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 1 cut(s) 1070
Hin6I GCGC 2 cut(s) 198, 864
HinP1I GCGC 2 cut(s) 198, 864
HphI GGTGA 4 cut(s) 262, 451, 846, 919
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 8 cut(s) 229, 448, 568, 579, 687, 809, 881, 1327
Hpy188III TCNNGA 6 cut(s) 193, 341, 707, 829, 988, 1176
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 6 cut(s) 452, 485, 698, 761, 820, 1336
HpyAV CCTTC 6 cut(s) 66, 199, 224, 387, 1152, 1262
HpyCH4III ACNGT 8 cut(s) 84, 131, 152, 485, 518, 740, 858, 1355
HpyCH4IV ACGT 5 cut(s) 394, 450, 593, 909, 1002
HpyCH4V TGCA 5 cut(s) 21, 120, 717, 765, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 762, 820, 872
HpyF3I CTNAG 5 cut(s) 212, 565, 1137, 1230, 1293
HpySE526I ACGT 5 cut(s) 394, 450, 593, 909, 1002
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 1 cut(s) 1070
HspAI GCGC 2 cut(s) 198, 864
Ksp22I TGATCA 1 cut(s) 205
LmnI GCTCC 2 cut(s) 236, 980
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 774
MaeI CTAG 2 cut(s) 14, 420
MaeII ACGT 5 cut(s) 394, 450, 593, 909, 1002
MaeIII GTNAC 5 cut(s) 25, 395, 658, 740, 1273
MbiI CCGCTC 1 cut(s) 917
MflI RGATCY 2 cut(s) 122, 1180
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 7 cut(s) 111, 324, 360, 453, 573, 1054, 1117
MmeI TCCRAC 2 cut(s) 471, 1362
MseI TTAA 5 cut(s) 347, 557, 842, 1116, 1404
MslI CAYNNNNRTG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 1403
MspR9I CCNGG 4 cut(s) 259, 463, 674, 1111
Mva1269I GAATGC 2 cut(s) 816, 1213
MvnI CGCG 1 cut(s) 869
MwoI GCNNNNNNNGC 3 cut(s) 762, 820, 872
NciI CCSGG 4 cut(s) 259, 463, 674, 1111
NlaIII CATG 1 cut(s) 1070
NmuCI GTSAC 1 cut(s) 395
NspV TTCGAA 1 cut(s) 1205
PceI AGGCCT 1 cut(s) 188
PcsI WCGNNNNNNNCGW 3 cut(s) 477, 654, 1211
PctI GAATGC 2 cut(s) 816, 1213
Pfl23II CGTACG 2 cut(s) 381, 952
PkrI GCNGC 4 cut(s) 337, 403, 868, 1228
Ple19I CGATCG 1 cut(s) 873
Ppu21I YACGTR 1 cut(s) 1003
PshBI ATTAAT 1 cut(s) 1116
Psp1406I AACGTT 1 cut(s) 593
PspLI CGTACG 2 cut(s) 381, 952
PsuI RGATCY 2 cut(s) 122, 1180
PvuI CGATCG 1 cut(s) 873
RgaI GCGATCGC 1 cut(s) 873
RsaI GTAC 7 cut(s) 104, 149, 252, 383, 545, 860, 954
RsaNI GTAC 7 cut(s) 103, 148, 251, 382, 544, 859, 953
RseI CAYNNNNRTG 1 cut(s) 770
SaqAI TTAA 5 cut(s) 347, 557, 842, 1116, 1404
SatI GCNGC 4 cut(s) 336, 402, 867, 1227
ScrFI CCNGG 4 cut(s) 259, 463, 674, 1111
SduI GDGCHC 1 cut(s) 241
SfaAI GCGATCGC 1 cut(s) 873
SfaNI GCATC 1 cut(s) 774
SfuI TTCGAA 1 cut(s) 1205
SgfI GCGATCGC 1 cut(s) 873
SgrAI CRCCGGYG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 3 cut(s) 63, 339, 1403
SmoI CTYRAG 3 cut(s) 63, 339, 1403
SnaBI TACGTA 1 cut(s) 1003
Sse9I AATT 7 cut(s) 111, 324, 360, 453, 573, 1054, 1117
SseBI AGGCCT 1 cut(s) 188
SspMI CTAG 2 cut(s) 14, 420
StuI AGGCCT 1 cut(s) 188
StyD4I CCNGG 4 cut(s) 257, 461, 672, 1109
TaaI ACNGT 8 cut(s) 84, 131, 152, 485, 518, 740, 858, 1355
TaiI ACGT 5 cut(s) 397, 453, 596, 912, 1005
TaqI TCGA 4 cut(s) 480, 732, 903, 1205
TasI AATT 7 cut(s) 111, 324, 360, 453, 573, 1054, 1117
TatI WGTACW 1 cut(s) 102
TauI GCSGC 3 cut(s) 404, 869, 1229
Tru1I TTAA 5 cut(s) 347, 557, 842, 1116, 1404
Tru9I TTAA 5 cut(s) 347, 557, 842, 1116, 1404
TscAI CASTG 2 cut(s) 136, 633
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 3 cut(s) 17, 1028, 1043
TspGWI ACGGA 5 cut(s) 399, 711, 810, 824, 970
TspRI CASTG 2 cut(s) 136, 633
Vha464I CTTAAG 1 cut(s) 1403
VspI ATTAAT 1 cut(s) 1116
XspI CTAG 2 cut(s) 14, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.