Rh5DG147100

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005230
Physical Location & Seq
Reverse (-)
14995862 .. 15000351
4490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG147100.1

Sequence Viewer

Length: 1731 bp
ATGAAAACCCTTCAGGTGATGAGATCACTACTGTTAATACATGTTGTGGGCTGCTTACTCCTTATTGGAGGGGGTGACAGCAGAAAAGTATTCAAGCTCTCCGAGGAGAAATCCGAATCTGAATCCACCTTCGAGTACAATGCCATCAGTTGCAGAGCCCACAGCGCTGCGCTCACTGATTTCGGAGGAGTAGGAGACGGCAAGACATCAAACACAAAGGCCTTTGAAGCCGCAATCAACCACCTGAGTCAGTACTCAACACCCAAGGAGGCGCTCAGCTTTACGTTCTGGCCGGGAAACCCTCGAGAATGGCCGGTGCTCAAACCATTCCCGTCTTACGGCAGAGGAAGGGACGCACCAGGTGGACGCTACGCCAGCCTCATATTCGGCACCAACCTCACCGACGTCGTCATCACCGGAGACAACGGCACAATCGACGGTCAAGCTCCGGTGAGGTCTCCGAACACAGATGGAATTAATCCGGATTCATGCACAAACGTCAAGATTGAGGACTGTTACATTGTCTCCGGCGACCACTGCGTGGCCGTCAAGAGCGGTTGGGACCAGTACGGCGTGTCGTTGGGTATGCCAACCAAGCAACTAGTGATCAGACGACTTACTTGTGTTTCGCCTTACAGCGCCGCCATTGCATTGGGCAGCGAGATGTCCGGCGGCATTGAAGACGTCAGAGCAGAAGACATCGTGGCCGTTGATAAGGAATCCGGGGTGAGGATCAAGACAGCTGTAGGGCGAGGAGGGTACGTGAGGGACGTGTGCTTAAGGAGAATGGAAATGCACACCATGATGTGGGCGTTTAGGATGAGTGGCGATTATGGGTCACACGCTGACGATGGCTATGACAAGAAGGCGATTCCTGAGATCAAAGGGATTCACTACAGGGATGTGGTGGCTGATAATGTGACGATGGCGGCGAAAATGGAGGGGATTGCTGGCCATCCGTTTACGGGGATTTGCATGTCGAATGTGACAGTTGGATTGGCTGCCAAGGCAAAGAAGCAGCCGTGGAGCTGTGCTGATATTGAGGGGGTTGCAAGCGGCGTGACGCCGACACCGTGTGATTTGCTACACGGCGATGATCCGAGTCAGGGTGATGTTGCTAGTGGTGGCTGTGATTTTCCTGTGGATGTTGTACCAATTGACATGGTGGAGATGAAAAGACGTAGGTATGATAGTAGGATGAAGTCGCCTCTTTCATCTCCTAGTCGTTCTAGGAGTCCAAGTTCGTCTGATCAGGACCCTGCGGACAAGGATCCAGCTCCGAGTCCATCATCATTGGGAGGGCACGAGCTTCATCACTACTATTCAACACCGCCAAAACCAGCTGATGATGATGATGATAACGTGACGTCAGAATCACCATCAACAAGTTTCGCTGGGCCTGATGCACCTTATGACATTCATGGTGGACGATCATCATCAAAAGGAAAGCCCAAAAAATGGAAGCCGAAGCGGTCACCACCACCTCCTCCTTCTCCTGCTGCTCCTGCGCCATTGCCTCCTAAGAATAATGCAAGCGCCGCTGCTGAGATGGTTCTTGATCGAGCTGTGCAGACTGCAGACTGCAGAAGACTGAAGAGATTGGTCTTGCTTGGCCTTGTTTTATATCATGGATTCTCTGAAAAGTATACCTGCTTTGATATCAAAATGGTTATCAGCTTGCGTCTCTATGGTTGTTTCCTTGTTGCTTCTGGCCTTCCCTGGCTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

576

Amino Acids

61.96

Weight (kDa)

6.71

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 144 - 348 1.7e-18 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 408, 687, 1370
Acc36I ACCTGC 1 cut(s) 1658
AccB1I GGYRCC 1 cut(s) 389
AccB7I CCANNNNNTGG 3 cut(s) 541, 807, 1458
AccBSI CCGCTC 1 cut(s) 555
AccI GTMKAC 1 cut(s) 1646
AccIII TCCGGA 1 cut(s) 481
AclWI GGATC 4 cut(s) 740, 1091, 1265, 1278
AcoI YGGCCR 5 cut(s) 290, 311, 543, 705, 952
AcuI CTGAAG 1 cut(s) 1613
AcyI GRCGYC 4 cut(s) 405, 684, 1064, 1367
AdeI CACNNNGTG 3 cut(s) 362, 541, 1076
AfaI GTAC 5 cut(s) 137, 254, 569, 761, 1152
AfeI AGCGCT 1 cut(s) 166
AfiI CCNNNNNNNGG 7 cut(s) 338, 541, 729, 807, 965, 1106, 1458
AflII CTTAAG 1 cut(s) 778
AflIII ACRYGT 2 cut(s) 40, 771
AgsI TTSAA 4 cut(s) 94, 227, 680, 1326
AhlI ACTAGT 1 cut(s) 601
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 2 cut(s) 358, 1718
AloI GAACNNNNNNTCC 2 cut(s) 1225, 1257
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 5 cut(s) 189, 414, 462, 529, 1688
AlwI GGATC 4 cut(s) 740, 1091, 1265, 1278
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 481
Aor51HI AGCGCT 1 cut(s) 166
AoxI GGCC 9 cut(s) 219, 290, 311, 543, 705, 952, 1397, 1612, 1711
ApeKI GCWGC 7 cut(s) 51, 167, 657, 1001, 1018, 1499, 1541
AseI ATTAAT 1 cut(s) 477
AspLEI GCGC 6 cut(s) 167, 172, 274, 641, 1510, 1538
AspS9I GGNCC 3 cut(s) 562, 1255, 1397
AsuC2I CCSGG 2 cut(s) 294, 724
AsuHPI GGTGA 9 cut(s) 28, 86, 391, 406, 463, 739, 1121, 1368, 1467
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 2 cut(s) 562, 1255
BaeGI GKGCMC 1 cut(s) 1305
BalI TGGCCA 1 cut(s) 954
BamHI GGATCC 1 cut(s) 1270
BanI GGYRCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 160
BauI CACGAG 1 cut(s) 1304
BbsI GAAGAC 3 cut(s) 687, 702, 1594
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 7 cut(s) 38, 154, 669, 988, 1030, 1486, 1528
BccI CCATC 8 cut(s) 152, 464, 845, 919, 963, 1294, 1387, 1543
BceAI ACGGC 8 cut(s) 214, 355, 442, 530, 586, 692, 1006, 1105
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 2 cut(s) 360, 1720
BclI TGATCA 2 cut(s) 606, 1249
BcnI CCSGG 2 cut(s) 294, 724
BcoDI GTCTC 5 cut(s) 189, 414, 462, 529, 1688
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 4 cut(s) 602, 1119, 1221, 1230
BfmI CTRYAG 4 cut(s) 744, 895, 1575, 1582
BfoI RGCGCY 4 cut(s) 168, 275, 642, 1539
BfrI CTTAAG 1 cut(s) 778
BfuAI ACCTGC 1 cut(s) 1658
BlpI GCTNAGC 1 cut(s) 275
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 4 cut(s) 294, 360, 724, 1720
Bme18I GGWCC 2 cut(s) 562, 1255
BmeT110I CYCGRG 1 cut(s) 303
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 3 cut(s) 562, 1255, 1397
BmiI GGNNCC 4 cut(s) 391, 563, 1257, 1272
BmrFI CCNGG 4 cut(s) 294, 360, 724, 1720
BmsI GCATC 1 cut(s) 1393
BpiI GAAGAC 3 cut(s) 687, 702, 1594
Bpu1102I GCTNAGC 1 cut(s) 275
BpuMI CCSGG 2 cut(s) 294, 724
BsaAI YACGTR 1 cut(s) 763
BsaBI GATNNNNATC 1 cut(s) 1435
BsaHI GRCGYC 4 cut(s) 405, 684, 1064, 1367
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 6 cut(s) 102, 264, 723, 1005, 1022, 1718
BsaWI WCCGGW 3 cut(s) 416, 448, 481
BsaXI ACNNNNNCTCC 6 cut(s) 509, 539, 747, 777, 1225, 1255
Bsc4I CCNNNNNNNGG 7 cut(s) 338, 541, 729, 807, 965, 1106, 1458
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 2 cut(s) 645, 1511
Bse8I GATNNNNATC 1 cut(s) 1435
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 2 cut(s) 360, 1720
BseDI CCNNGG 6 cut(s) 102, 264, 723, 1005, 1022, 1718
BseGI GGATG 5 cut(s) 825, 907, 955, 1150, 1203
BseJI GATNNNNATC 1 cut(s) 1435
BseLI CCNNNNNNNGG 7 cut(s) 338, 541, 729, 807, 965, 1106, 1458
BseMI GCAATG 2 cut(s) 645, 1511
BseMII CTCAG 4 cut(s) 236, 289, 867, 1536
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 4 cut(s) 119, 201, 768, 1476
BseSI GKGCMC 1 cut(s) 1305
BseXI GCAGC 7 cut(s) 38, 154, 669, 988, 1030, 1486, 1528
BseYI CCCAGC 1 cut(s) 1394
BsgI GTGCAG 1 cut(s) 1589
BshFI GGCC 9 cut(s) 221, 292, 313, 545, 707, 954, 1399, 1614, 1713
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 8 cut(s) 293, 314, 417, 449, 482, 528, 669, 723
BslFI GGGAC 3 cut(s) 365, 575, 782
BslI CCNNNNNNNGG 7 cut(s) 338, 541, 729, 807, 965, 1106, 1458
BsmAI GTCTC 5 cut(s) 189, 414, 462, 529, 1688
BsmBI CGTCTC 2 cut(s) 189, 1688
BsmFI GGGAC 3 cut(s) 365, 575, 782
BsnI GGCC 9 cut(s) 221, 292, 313, 545, 707, 954, 1399, 1614, 1713
Bso31I GGTCTC 1 cut(s) 462
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 3 cut(s) 160, 321, 1305
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
Bsp1720I GCTNAGC 1 cut(s) 275
BspANI GGCC 9 cut(s) 221, 292, 313, 545, 707, 954, 1399, 1614, 1713
BspCNI CTCAG 4 cut(s) 237, 288, 868, 1537
BspEI TCCGGA 1 cut(s) 481
BspLI GGNNCC 4 cut(s) 391, 563, 1257, 1272
BspMAI CTGCAG 2 cut(s) 1579, 1586
BspMI ACCTGC 1 cut(s) 1658
BspPI GGATC 4 cut(s) 740, 1091, 1265, 1278
BspT107I GGYRCC 1 cut(s) 389
BspTI CTTAAG 1 cut(s) 778
BspTNI GGTCTC 1 cut(s) 462
BsrBI CCGCTC 1 cut(s) 555
BsrDI GCAATG 2 cut(s) 645, 1511
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 6 cut(s) 102, 264, 723, 1005, 1022, 1718
BssMI GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
BssNAI GTATAC 1 cut(s) 1647
BssNI GRCGYC 4 cut(s) 405, 684, 1064, 1367
BssSI CACGAG 1 cut(s) 1304
BssT1I CCWWGG 2 cut(s) 264, 1005
Bst1107I GTATAC 1 cut(s) 1647
Bst2BI CACGAG 1 cut(s) 1304
Bst2UI CCWGG 2 cut(s) 360, 1720
Bst4CI ACNGT 5 cut(s) 33, 440, 515, 991, 1074
Bst6I CTCTTC 1 cut(s) 1589
BstACI GRCGYC 4 cut(s) 405, 684, 1064, 1367
BstAFI CTTAAG 1 cut(s) 778
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 5 cut(s) 376, 952, 1054, 1534, 1679
BstDEI CTNAG 5 cut(s) 245, 275, 876, 1521, 1545
BstDSI CCRYGG 1 cut(s) 1022
BstEII GGTNACC 1 cut(s) 1473
BstF5I GGATG 5 cut(s) 825, 907, 955, 1150, 1203
BstH2I RGCGCY 4 cut(s) 168, 275, 642, 1539
BstHHI GCGC 6 cut(s) 167, 172, 274, 641, 1510, 1538
BstKTI GATC 9 cut(s) 26, 609, 735, 882, 1099, 1252, 1273, 1433, 1561
BstMAI GTCTC 5 cut(s) 189, 414, 462, 529, 1688
BstMBI GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
BstMWI GCNNNNNNNGC 9 cut(s) 164, 227, 375, 537, 595, 647, 1007, 1505, 1538
BstNI CCWGG 2 cut(s) 360, 1720
BstNSI RCATGY 2 cut(s) 44, 979
BstPI GGTNACC 1 cut(s) 1473
BstSCI CCNGG 4 cut(s) 292, 358, 722, 1718
BstSFI CTRYAG 4 cut(s) 744, 895, 1575, 1582
BstSLI GKGCMC 1 cut(s) 1305
BstV1I GCAGC 7 cut(s) 38, 154, 669, 988, 1030, 1486, 1528
BstV2I GAAGAC 3 cut(s) 687, 702, 1594
BstX2I RGATCY 1 cut(s) 1270
BstXI CCANNNNNNTGG 1 cut(s) 652
BstYI RGATCY 1 cut(s) 1270
BstZ17I GTATAC 1 cut(s) 1647
BsuRI GGCC 9 cut(s) 221, 292, 313, 545, 707, 954, 1399, 1614, 1713
BtgI CCRYGG 1 cut(s) 1022
BtgZI GCGATG 1 cut(s) 1107
BtrI CACGTC 1 cut(s) 772
BtsCI GGATG 5 cut(s) 825, 907, 955, 1150, 1203
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 2 cut(s) 174, 535
BveI ACCTGC 1 cut(s) 1658
Cac8I GCNNGC 5 cut(s) 376, 952, 1054, 1534, 1679
CfoI GCGC 6 cut(s) 167, 172, 274, 641, 1510, 1538
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 3 cut(s) 562, 1255, 1397
CseI GACGC 4 cut(s) 362, 375, 1072, 1670
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 5 cut(s) 136, 253, 568, 760, 1151
CviAII CATG 7 cut(s) 41, 489, 802, 976, 1162, 1421, 1628
CviQI GTAC 5 cut(s) 136, 253, 568, 760, 1151
DdeI CTNAG 5 cut(s) 245, 275, 876, 1521, 1545
DpnI GATC 9 cut(s) 25, 608, 734, 881, 1098, 1251, 1272, 1432, 1560
DpnII GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
DraIII CACNNNGTG 3 cut(s) 362, 541, 1076
EaeI YGGCCR 5 cut(s) 290, 311, 543, 705, 952
Eam1104I CTCTTC 1 cut(s) 1589
EarI CTCTTC 1 cut(s) 1589
Eco130I CCWWGG 2 cut(s) 264, 1005
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 462
Eco32I GATATC 1 cut(s) 1660
Eco47I GGWCC 2 cut(s) 562, 1255
Eco47III AGCGCT 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 1613
Eco88I CYCGRG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 1473
EcoO109I RGGNCCY 1 cut(s) 1255
EcoO65I GGTNACC 1 cut(s) 1473
EcoRII CCWGG 2 cut(s) 358, 1718
EcoRV GATATC 1 cut(s) 1660
EcoT14I CCWWGG 2 cut(s) 264, 1005
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 264, 1005
Esp3I CGTCTC 2 cut(s) 189, 1688
FaeI CATG 7 cut(s) 44, 492, 805, 979, 1165, 1424, 1631
FaqI GGGAC 3 cut(s) 365, 575, 782
FatI CATG 7 cut(s) 40, 488, 801, 975, 1161, 1420, 1627
FbaI TGATCA 2 cut(s) 606, 1249
FblI GTMKAC 1 cut(s) 1646
FokI GGATG 5 cut(s) 832, 914, 942, 1157, 1210
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 4 cut(s) 602, 1119, 1221, 1230
GlaI GCGC 6 cut(s) 166, 171, 273, 640, 1509, 1537
GsaI CCCAGC 1 cut(s) 1398
HaeII RGCGCY 4 cut(s) 168, 275, 642, 1539
HaeIII GGCC 9 cut(s) 221, 292, 313, 545, 707, 954, 1399, 1614, 1713
HapII CCGG 8 cut(s) 293, 314, 417, 449, 482, 528, 669, 723
HgaI GACGC 4 cut(s) 362, 375, 1072, 1670
HhaI GCGC 6 cut(s) 167, 172, 274, 641, 1510, 1538
Hin1I GRCGYC 4 cut(s) 405, 684, 1064, 1367
Hin1II CATG 7 cut(s) 44, 492, 805, 979, 1165, 1424, 1631
Hin6I GCGC 6 cut(s) 165, 170, 272, 639, 1508, 1536
HinP1I GCGC 6 cut(s) 165, 170, 272, 639, 1508, 1536
HpaII CCGG 8 cut(s) 293, 314, 417, 449, 482, 528, 669, 723
HphI GGTGA 9 cut(s) 28, 86, 391, 406, 463, 739, 1121, 1368, 1467
Hpy166II GTNNAC 4 cut(s) 365, 963, 1427, 1647
Hpy188III TCNNGA 8 cut(s) 305, 482, 502, 550, 736, 875, 1253, 1556
Hpy8I GTNNAC 4 cut(s) 365, 963, 1427, 1647
Hpy99I CGWCG 3 cut(s) 407, 410, 440
HpyAV CCTTC 6 cut(s) 20, 139, 342, 859, 1500, 1724
HpyCH4III ACNGT 5 cut(s) 33, 440, 515, 991, 1074
HpyCH4IV ACGT 9 cut(s) 284, 405, 498, 684, 762, 771, 1180, 1362, 1367
HpyF10VI GCNNNNNNNGC 9 cut(s) 164, 227, 375, 537, 595, 647, 1007, 1505, 1538
HpyF3I CTNAG 5 cut(s) 245, 275, 876, 1521, 1545
HpySE526I ACGT 9 cut(s) 284, 405, 498, 684, 762, 771, 1180, 1362, 1367
Hsp92I GRCGYC 4 cut(s) 405, 684, 1064, 1367
Hsp92II CATG 7 cut(s) 44, 492, 805, 979, 1165, 1424, 1631
HspAI GCGC 6 cut(s) 165, 170, 272, 639, 1508, 1536
Kpn2I TCCGGA 1 cut(s) 481
Ksp22I TGATCA 2 cut(s) 606, 1249
Kzo9I GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
LmnI GCTCC 4 cut(s) 451, 1026, 1282, 1507
Lsp1109I GCAGC 7 cut(s) 38, 154, 669, 988, 1030, 1486, 1528
LweI GCATC 1 cut(s) 1393
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 4 cut(s) 602, 1119, 1221, 1230
MaeII ACGT 9 cut(s) 284, 405, 498, 684, 762, 771, 1180, 1362, 1367
MaeIII GTNAC 8 cut(s) 74, 515, 837, 919, 985, 1060, 1363, 1473
MalI GATC 9 cut(s) 25, 608, 734, 881, 1098, 1251, 1272, 1432, 1560
MbiI CCGCTC 1 cut(s) 555
MboI GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
MboII GAAGA 4 cut(s) 692, 707, 1599, 1606
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 1 cut(s) 1270
MhlI GDGCHC 3 cut(s) 160, 321, 1305
MlsI TGGCCA 1 cut(s) 954
MluCI AATT 2 cut(s) 474, 1155
MluNI TGGCCA 1 cut(s) 954
MlyI GAGTC 4 cut(s) 256, 1111, 1243, 1291
MmeI TCCRAC 1 cut(s) 973
Mox20I TGGCCA 1 cut(s) 954
MroI TCCGGA 1 cut(s) 481
MscI TGGCCA 1 cut(s) 954
MseI TTAA 4 cut(s) 35, 477, 779, 1729
MslI CAYNNNNRTG 2 cut(s) 803, 1092
Msp20I TGGCCA 1 cut(s) 954
MspA1I CMGCKG 3 cut(s) 743, 1343, 1541
MspCI CTTAAG 1 cut(s) 778
MspI CCGG 8 cut(s) 293, 314, 417, 449, 482, 528, 669, 723
MspR9I CCNGG 4 cut(s) 294, 360, 724, 1720
MteI GCGCNGCGC 1 cut(s) 168
MunI CAATTG 1 cut(s) 1155
MvaI CCWGG 2 cut(s) 360, 1720
MwoI GCNNNNNNNGC 9 cut(s) 164, 227, 375, 537, 595, 647, 1007, 1505, 1538
NciI CCSGG 2 cut(s) 294, 724
NdeII GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
NlaIII CATG 7 cut(s) 44, 492, 805, 979, 1165, 1424, 1631
NlaIV GGNNCC 4 cut(s) 391, 563, 1257, 1272
NmuCI GTSAC 7 cut(s) 74, 837, 919, 985, 1060, 1363, 1473
NspI RCATGY 2 cut(s) 44, 979
PaeR7I CTCGAG 1 cut(s) 303
PceI AGGCCT 1 cut(s) 221
PciI ACATGT 1 cut(s) 40
PcsI WCGNNNNNNNCGW 4 cut(s) 432, 768, 929, 1070
PfeI GAWTC 8 cut(s) 116, 122, 485, 719, 871, 889, 1373, 1632
PflFI GACNNNGTC 1 cut(s) 407
PflMI CCANNNNNTGG 3 cut(s) 541, 807, 1458
PleI GAGTC 4 cut(s) 255, 1110, 1242, 1290
PpsI GAGTC 4 cut(s) 255, 1110, 1242, 1290
Ppu21I YACGTR 1 cut(s) 763
PpuMI RGGWCCY 1 cut(s) 1255
PscI ACATGT 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 1255
Psp6I CCWGG 2 cut(s) 358, 1718
PspEI GGTNACC 1 cut(s) 1473
PspFI CCCAGC 1 cut(s) 1394
PspGI CCWGG 2 cut(s) 358, 1718
PspN4I GGNNCC 4 cut(s) 391, 563, 1257, 1272
PspPI GGNCC 3 cut(s) 562, 1255, 1397
PspPPI RGGWCCY 1 cut(s) 1255
PstI CTGCAG 2 cut(s) 1579, 1586
PsuI RGATCY 1 cut(s) 1270
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 2 cut(s) 743, 1343
RsaI GTAC 5 cut(s) 137, 254, 569, 761, 1152
RsaNI GTAC 5 cut(s) 136, 253, 568, 760, 1151
RseI CAYNNNNRTG 2 cut(s) 803, 1092
SaqAI TTAA 4 cut(s) 35, 477, 779, 1729
Sau3AI GATC 9 cut(s) 23, 606, 732, 879, 1096, 1249, 1270, 1430, 1558
Sau96I GGNCC 3 cut(s) 562, 1255, 1397
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 4 cut(s) 256, 1111, 1243, 1291
ScrFI CCNGG 4 cut(s) 294, 360, 724, 1720
SduI GDGCHC 3 cut(s) 160, 321, 1305
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 1 cut(s) 1393
SfcI CTRYAG 4 cut(s) 744, 895, 1575, 1582
Sfr274I CTCGAG 1 cut(s) 303
SinI GGWCC 2 cut(s) 562, 1255
SlaI CTCGAG 1 cut(s) 303
SmiMI CAYNNNNRTG 2 cut(s) 803, 1092
SmlI CTYRAG 2 cut(s) 303, 778
SmoI CTYRAG 2 cut(s) 303, 778
SpeI ACTAGT 1 cut(s) 601
Sse9I AATT 2 cut(s) 474, 1155
SseBI AGGCCT 1 cut(s) 221
SspMI CTAG 4 cut(s) 602, 1119, 1221, 1230
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 4 cut(s) 292, 358, 722, 1718
StyI CCWWGG 2 cut(s) 264, 1005
TaaI ACNGT 5 cut(s) 33, 440, 515, 991, 1074
TaiI ACGT 9 cut(s) 287, 408, 501, 687, 765, 774, 1183, 1365, 1370
TaqI TCGA 5 cut(s) 132, 304, 435, 980, 1561
TasI AATT 2 cut(s) 474, 1155
TatI WGTACW 2 cut(s) 135, 252
TauI GCSGC 6 cut(s) 233, 644, 675, 932, 1059, 1541
TfiI GAWTC 8 cut(s) 116, 122, 485, 719, 871, 889, 1373, 1632
Tru1I TTAA 4 cut(s) 35, 477, 779, 1729
Tru9I TTAA 4 cut(s) 35, 477, 779, 1729
TscAI CASTG 2 cut(s) 181, 542
TseFI GTSAC 7 cut(s) 74, 837, 919, 985, 1060, 1363, 1473
TseI GCWGC 7 cut(s) 51, 167, 657, 1001, 1018, 1499, 1541
Tsp45I GTSAC 7 cut(s) 74, 837, 919, 985, 1060, 1363, 1473
TspDTI ATGAA 7 cut(s) 17, 477, 1187, 1203, 1214, 1301, 1409
TspGWI ACGGA 1 cut(s) 948
TspRI CASTG 2 cut(s) 181, 542
Tth111I GACNNNGTC 1 cut(s) 407
Van91I CCANNNNNTGG 3 cut(s) 541, 807, 1458
Vha464I CTTAAG 1 cut(s) 778
VpaK11BI GGWCC 2 cut(s) 562, 1255
VspI ATTAAT 1 cut(s) 477
XceI RCATGY 2 cut(s) 44, 979
XhoI CTCGAG 1 cut(s) 303
XmiI GTMKAC 1 cut(s) 1646
XspI CTAG 4 cut(s) 602, 1119, 1221, 1230
ZraI GACGTC 3 cut(s) 406, 685, 1368
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.