RchiOBHm_Chr5g0020291

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006921
Physical Location & Seq
Reverse (-)
14512615 .. 14515694
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30039

Sequence Viewer

Length: 1506 bp
ATGGAGAACACATCCACTTTGGTGGAACACATGCATTCCGTTGAAAGCTCACAAAAGAGATCATATATCAATTCTGTGAAGAAAACACCTACTTCATCAACATCAAAAGGGTCATCAGCTTGTGCCTCAAGGGTTGCTTCCTTGTTGCTTGTTCTGTTCAGGGCAATGCAGTACTTAATCCAACCCATTATGAGACTTCTTCATCCAATCACCCATGCTTTCTCCAAAAGCCGATCATCAGCTGATGAGCTCATGATCATGCAAATTGAAGAATACCATGGCATGAGCACTCATAATAAAGTGTTCGAAGCTTCAGAGCTTTACTTGCACAACAAGATGAGCCCTTCCACTAGACTCCTCAAAATTGTACAAACTCAGAAAGAGACTAAGTTTGCCATCCATTTCTCAAACAACCAAGAGTTTGAAGACATTTATGAAGGAGTCCAGCTGAAATGGAAATACAATTCCATATCCAAGAAGTCCAAATATTATCCTAGCAAAGCAGGGAAAAAGCAGCAATTCTTTGAGCTCAGATTTGATGTCAAACACAAGGAAAAGGTCATGGATGCTTACTTGCCTTATGTTGTAGAGACATTCAAGGCTATGAAAGATGCAGGAAAGATTGTCAAGCTGCATACACTTGTAAGGTCAGAGGATTACAAAGTAAGATGGAGTGCAATCAATCTCGAACACCCTGCAACATTCGAGACCATAGCCATGGATTCTGAGCAGAAGAGATCGGTTTTGGAGGATTTGGACAGGTTTGTTAAGAGGAAAGAGTTCTATCAAAGAGTAGGAAGGGCTTGGAAAAGGGGTTATCTACTTTATGGTCCTCCAGGGACTGGTAAATCATCCCTTATAGGTGCTATTGCTAATTATCTGAAGTTTGATATTTATGACTTGCAACTCACGCATTTGATGTCGGATATGGAATTAAGAAGAGTGTTGCTGGCAACAACAAATAAATCCATACTTGTGATTGAGGATATAGATTGTAGTCAATTGAAGCTGCAAGATAGAAAGAACAGCAATAAGAAAAATGACTCAGAAAGCAATCAGCTTTCGCTATTGGGACGACTACTGAACTTCACAGATGGCCTGTGGTATAGTTGTGGAGAAGAGAGAATCATTATATTCACTACCAGTCACAAGAGCAAACTAGACCCTGCATTGCTGCGTTCAGGACGAATGGACATGCACGTCCACATGTCGTATTGCTCATATGAGGGCTTCAAAACATTGGCCTCAAATTACCTAGGGATCAAGGAACACCAACCTTTGTTTGATGAGATTGAAAGCCTACTGCAGAAGACAGAGGTTTCTCCTGCACAAGTGACCGAGGAGCTGATGAAGAGTGAGATTGCTGATGTTGCACTCCAAGCTTTGATCACAGGGATAAAGGAAATGAATAAAAGGGAGGAAGATGAAAAAAAGGCCAAAGATGAAGAGAGAGTTAAGAACGATAAAGAGAAGGTTGATCATAAACAGTTGACATATTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

501

Amino Acids

57.99

Weight (kDa)

9.02

Isoelectric Point (pI)

53.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 70 - 154 3.1e-15 Domain associated at C-terminal with AAA
AAA PF00004 273 - 404 1.3e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 406 - 478 9.5e-23 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1199
AccB7I CCANNNNNTGG 1 cut(s) 842
AclWI GGATC 1 cut(s) 1268
AcuI CTGAAG 2 cut(s) 297, 902
AfaI GTAC 2 cut(s) 173, 369
AfiI CCNNNNNNNGG 1 cut(s) 842
AflIII ACRYGT 1 cut(s) 1206
AgsI TTSAA 7 cut(s) 44, 269, 425, 598, 1006, 1234, 1295
AjiI CACGTC 1 cut(s) 1201
AjnI CCWGG 1 cut(s) 835
AleI CACNNNNGTG 1 cut(s) 20
Alw21I GWGCWC 3 cut(s) 252, 290, 531
Alw26I GTCTC 4 cut(s) 187, 377, 584, 701
AlwI GGATC 1 cut(s) 1268
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 3 cut(s) 1096, 1242, 1434
ApeKI GCWGC 4 cut(s) 514, 631, 1009, 1174
Asp700I GAANNNNTTC 1 cut(s) 779
AspA2I CCTAGG 1 cut(s) 1255
AspS9I GGNCC 1 cut(s) 830
AsuHPI GGTGA 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 306
AvaII GGWCC 1 cut(s) 830
AvrII CCTAGG 1 cut(s) 1255
BanII GRGCYC 3 cut(s) 252, 344, 531
BbsI GAAGAC 2 cut(s) 432, 1316
Bbv12I GWGCWC 3 cut(s) 252, 290, 531
BbvI GCAGC 4 cut(s) 526, 618, 996, 1161
BccI CCATC 3 cut(s) 404, 663, 1088
BcgI CGANNNNNNTGC 2 cut(s) 677, 711
BciT130I CCWGG 1 cut(s) 837
BclI TGATCA 3 cut(s) 255, 1386, 1477
BcoDI GTCTC 4 cut(s) 187, 377, 584, 701
BfaI CTAG 4 cut(s) 351, 495, 1160, 1256
BfmI CTRYAG 1 cut(s) 1304
BisI GCNGC 4 cut(s) 515, 632, 1010, 1175
BlnI CCTAGG 1 cut(s) 1255
BlsI GCNGC 4 cut(s) 516, 633, 1011, 1176
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 1 cut(s) 830
BmgBI CACGTC 1 cut(s) 1201
BmgT120I GGNCC 1 cut(s) 830
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 2 cut(s) 556, 601
BpiI GAAGAC 2 cut(s) 432, 1316
BpmI CTGGAG 1 cut(s) 819
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 112
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 5 cut(s) 277, 717, 836, 1255, 1338
Bsc4I CCNNNNNNNGG 1 cut(s) 842
Bse1I ACTGG 2 cut(s) 847, 1143
Bse3DI GCAATG 2 cut(s) 171, 1169
BseBI CCWGG 1 cut(s) 837
BseDI CCNNGG 5 cut(s) 277, 717, 836, 1255, 1338
BseGI GGATG 5 cut(s) 11, 202, 396, 571, 851
BseLI CCNNNNNNNGG 1 cut(s) 842
BseMI GCAATG 2 cut(s) 171, 1169
BseMII CTCAG 4 cut(s) 389, 544, 717, 1059
BseNI ACTGG 2 cut(s) 847, 1143
BseRI GAGGAG 2 cut(s) 347, 1355
BseXI GCAGC 4 cut(s) 526, 618, 996, 1161
BsgI GTGCAG 1 cut(s) 1311
BshFI GGCC 3 cut(s) 1098, 1244, 1436
BsiHKAI GWGCWC 3 cut(s) 252, 290, 531
BslFI GGGAC 2 cut(s) 853, 1086
BslI CCNNNNNNNGG 1 cut(s) 842
BsmAI GTCTC 4 cut(s) 187, 377, 584, 701
BsmFI GGGAC 2 cut(s) 853, 1086
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 3 cut(s) 1098, 1244, 1436
Bso31I GGTCTC 1 cut(s) 701
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 4 cut(s) 252, 290, 344, 531
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
Bsp19I CCATGG 2 cut(s) 277, 717
BspANI GGCC 3 cut(s) 1098, 1244, 1436
BspCNI CTCAG 4 cut(s) 388, 543, 718, 1058
BspHI TCATGA 1 cut(s) 252
BspMAI CTGCAG 1 cut(s) 1308
BspPI GGATC 1 cut(s) 1268
BspT104I TTCGAA 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 171, 1169
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 2 cut(s) 847, 1143
BssECI CCNNGG 5 cut(s) 277, 717, 836, 1255, 1338
BssMI GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
BssT1I CCWWGG 3 cut(s) 277, 717, 1255
Bst2UI CCWGG 1 cut(s) 837
Bst4CI ACNGT 1 cut(s) 1488
Bst6I CTCTTC 5 cut(s) 728, 934, 1113, 1346, 1440
BstAUI TGTACA 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 5 cut(s) 375, 387, 530, 726, 1045
BstDSI CCRYGG 2 cut(s) 277, 717
BstF5I GGATG 5 cut(s) 11, 202, 396, 571, 851
BstKTI GATC 7 cut(s) 62, 236, 258, 740, 1263, 1389, 1480
BstMAI GTCTC 4 cut(s) 187, 377, 584, 701
BstMBI GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
BstMWI GCNNNNNNNGC 4 cut(s) 325, 910, 1370, 1379
BstNI CCWGG 1 cut(s) 837
BstNSI RCATGY 3 cut(s) 34, 1198, 1210
BstSCI CCNGG 1 cut(s) 835
BstSFI CTRYAG 1 cut(s) 1304
BstV1I GCAGC 4 cut(s) 526, 618, 996, 1161
BstV2I GAAGAC 2 cut(s) 432, 1316
BstXI CCANNNNNNTGG 2 cut(s) 22, 718
BsuRI GGCC 3 cut(s) 1098, 1244, 1436
BtgI CCRYGG 2 cut(s) 277, 717
BtrI CACGTC 1 cut(s) 1201
BtsCI GGATG 5 cut(s) 11, 202, 396, 571, 851
Cac8I GCNNGC 1 cut(s) 951
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 172, 368
CviQI GTAC 2 cut(s) 172, 368
DdeI CTNAG 5 cut(s) 375, 387, 530, 726, 1045
DpnI GATC 7 cut(s) 61, 235, 257, 739, 1262, 1388, 1479
DpnII GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
DrdI GACNNNNNNGTC 1 cut(s) 1199
DseDI GACNNNNNNGTC 1 cut(s) 1199
Eam1104I CTCTTC 5 cut(s) 728, 934, 1113, 1346, 1440
EarI CTCTTC 5 cut(s) 728, 934, 1113, 1346, 1440
Ecl136II GAGCTC 2 cut(s) 250, 529
Eco130I CCWWGG 3 cut(s) 277, 717, 1255
Eco24I GRGCYC 3 cut(s) 252, 344, 531
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 1 cut(s) 830
Eco53kI GAGCTC 2 cut(s) 250, 529
Eco57I CTGAAG 2 cut(s) 297, 902
EcoICRI GAGCTC 2 cut(s) 250, 529
EcoRII CCWGG 1 cut(s) 835
EcoT14I CCWWGG 3 cut(s) 277, 717, 1255
EcoT22I ATGCAT 1 cut(s) 36
EcoT38I GRGCYC 3 cut(s) 252, 344, 531
ErhI CCWWGG 3 cut(s) 277, 717, 1255
FalI AAGNNNNNCTT 2 cut(s) 121, 153
FaqI GGGAC 2 cut(s) 853, 1086
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 3 cut(s) 255, 1386, 1477
Fnu4HI GCNGC 4 cut(s) 515, 632, 1010, 1175
FokI GGATG 4 cut(s) 189, 383, 578, 838
FriOI GRGCYC 3 cut(s) 252, 344, 531
Fsp4HI GCNGC 4 cut(s) 515, 632, 1010, 1175
FspBI CTAG 4 cut(s) 351, 495, 1160, 1256
GluI GCNGC 4 cut(s) 515, 632, 1010, 1175
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 3 cut(s) 1098, 1244, 1436
HincII GTYRAC 1 cut(s) 1491
HindII GTYRAC 1 cut(s) 1491
HindIII AAGCTT 2 cut(s) 309, 1380
HinfI GANTC 5 cut(s) 354, 441, 722, 1043, 1125
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 2 cut(s) 1204, 1491
Hpy188I TCNGA 8 cut(s) 316, 378, 533, 652, 727, 882, 925, 1048
Hpy188III TCNNGA 4 cut(s) 253, 686, 706, 1182
Hpy8I GTNNAC 2 cut(s) 1204, 1491
HpyAV CCTTC 4 cut(s) 354, 431, 792, 1465
HpyCH4III ACNGT 1 cut(s) 1488
HpyCH4IV ACGT 1 cut(s) 1200
HpyF10VI GCNNNNNNNGC 4 cut(s) 325, 910, 1370, 1379
HpyF3I CTNAG 5 cut(s) 375, 387, 530, 726, 1045
HpySE526I ACGT 1 cut(s) 1200
Ksp22I TGATCA 3 cut(s) 255, 1386, 1477
Kzo9I GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
LmnI GCTCC 1 cut(s) 1342
Lsp1109I GCAGC 4 cut(s) 526, 618, 996, 1161
LweI GCATC 2 cut(s) 556, 601
MaeI CTAG 4 cut(s) 351, 495, 1160, 1256
MaeII ACGT 1 cut(s) 1200
MaeIII GTNAC 2 cut(s) 1145, 1333
MalI GATC 7 cut(s) 61, 235, 257, 739, 1262, 1388, 1479
MboI GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
MfeI CAATTG 1 cut(s) 1001
MhlI GDGCHC 4 cut(s) 252, 290, 344, 531
MluCI AATT 9 cut(s) 70, 264, 363, 463, 518, 874, 932, 1001, 1249
MlyI GAGTC 3 cut(s) 348, 450, 1037
MmeI TCCRAC 2 cut(s) 205, 903
Mph1103I ATGCAT 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 779
MseI TTAA 4 cut(s) 176, 768, 935, 1455
MslI CAYNNNNRTG 4 cut(s) 20, 257, 716, 974
MspA1I CMGCKG 2 cut(s) 242, 448
MspR9I CCNGG 1 cut(s) 837
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 34
MvaI CCWGG 1 cut(s) 837
MwoI GCNNNNNNNGC 4 cut(s) 325, 910, 1370, 1379
NcoI CCATGG 2 cut(s) 277, 717
NdeI CATATG 1 cut(s) 1222
NdeII GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
NmuCI GTSAC 2 cut(s) 1145, 1333
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 3 cut(s) 34, 1198, 1210
NspV TTCGAA 1 cut(s) 306
OliI CACNNNNGTG 1 cut(s) 20
PagI TCATGA 1 cut(s) 252
PciI ACATGT 1 cut(s) 1206
PctI GAATGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 779
PfeI GAWTC 2 cut(s) 722, 1125
PflMI CCANNNNNTGG 1 cut(s) 842
PkrI GCNGC 4 cut(s) 516, 633, 1011, 1176
PleI GAGTC 3 cut(s) 348, 449, 1037
PpsI GAGTC 3 cut(s) 348, 449, 1037
PscI ACATGT 1 cut(s) 1206
Psp124BI GAGCTC 2 cut(s) 252, 531
Psp6I CCWGG 1 cut(s) 835
PspGI CCWGG 1 cut(s) 835
PspPI GGNCC 1 cut(s) 830
PstI CTGCAG 1 cut(s) 1308
PstNI CAGNNNCTG 1 cut(s) 842
PvuII CAGCTG 2 cut(s) 242, 448
RsaI GTAC 2 cut(s) 173, 369
RsaNI GTAC 2 cut(s) 172, 368
RseI CAYNNNNRTG 4 cut(s) 20, 257, 716, 974
SacI GAGCTC 2 cut(s) 252, 531
SaqAI TTAA 4 cut(s) 176, 768, 935, 1455
SatI GCNGC 4 cut(s) 515, 632, 1010, 1175
Sau3AI GATC 7 cut(s) 59, 233, 255, 737, 1260, 1386, 1477
Sau96I GGNCC 1 cut(s) 830
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 3 cut(s) 348, 450, 1037
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 4 cut(s) 252, 290, 344, 531
SfaNI GCATC 2 cut(s) 556, 601
SfcI CTRYAG 1 cut(s) 1304
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 1 cut(s) 830
SmiMI CAYNNNNRTG 4 cut(s) 20, 257, 716, 974
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 9 cut(s) 70, 264, 363, 463, 518, 874, 932, 1001, 1249
SspI AATATT 1 cut(s) 488
SspMI CTAG 4 cut(s) 351, 495, 1160, 1256
SstI GAGCTC 2 cut(s) 252, 531
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 3 cut(s) 277, 717, 1255
TaaI ACNGT 1 cut(s) 1488
TaiI ACGT 1 cut(s) 1203
TaqI TCGA 3 cut(s) 306, 687, 705
TaqII GACCGA 1 cut(s) 1352
TasI AATT 9 cut(s) 70, 264, 363, 463, 518, 874, 932, 1001, 1249
TatI WGTACW 2 cut(s) 171, 367
TfiI GAWTC 2 cut(s) 722, 1125
Tru1I TTAA 4 cut(s) 176, 768, 935, 1455
Tru9I TTAA 4 cut(s) 176, 768, 935, 1455
TseFI GTSAC 2 cut(s) 1145, 1333
TseI GCWGC 4 cut(s) 514, 631, 1009, 1174
Tsp45I GTSAC 2 cut(s) 1145, 1333
TspDTI ATGAA 8 cut(s) 84, 191, 450, 620, 1364, 1421, 1440, 1458
TspGWI ACGGA 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 1 cut(s) 830
XceI RCATGY 3 cut(s) 34, 1198, 1210
XmaJI CCTAGG 1 cut(s) 1255
XmnI GAANNNNTTC 1 cut(s) 779
XspI CTAG 4 cut(s) 351, 495, 1160, 1256
ZrmI AGTACT 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.