Rw5G013170

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006921
Physical Location & Seq
Reverse (-)
15498810 .. 15500291
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013170.1

Sequence Viewer

Length: 1338 bp
ATGCAGTACTTAATCCAACCCATTATGAGACTTCTTCATCCAATCACCCATGCATTCTCCAAAAGCCGATCATCAGCTGATGAACTCATGATCATGCAAATTGAAGAATACCATGGCATGAGCACTCATAATAAAGTGTTCGAAGCTTCAGAGCTTTACTTGCACAACAAGATGAGCCCTTCCACTAGACTCCTCAAAATTGTACAAACTCAGAAAGAGACTAAGTTTGCCATCCATTTCTCAAACAACCAAGAGTTTGAAGACATTTATGAAGGAGTCCAGCTGAAATGGAAATACAATTCCAAATCCAAGAAGTCCAATTATCCTAGCAAAGCAGGGAAAAAGCAGCAATTCTTTGAGCTCAGATTTGATGTCAAACACAAGGAAAAGGTCATGGATGCTTACTTGCCTTATGTTGTAGAGACATTCAAGGCTATGAAAGATGCAGGAAAGATTGTCAAGCTGCATACACTTGTAAGGTCAGAGGATTACAAAGTAAGATGGAATGCAATCAATTTCGAACACCCTGCAACATTCGAGACCATAGCCATGGATTCTGAGCAGAAGAGATCGGTTTTGGAGGATTTGGACAGGTTTGTTAAGAGGAAAGAGTTCTATCAAAGAGTAGGAAGGGCTTGGAAAAGGGGGTATCTACTTTATGGTCCTCCAGGGACTGGTAAATCATCCCTTATAGGTGCTATTGCTAATTATCTGAAGTTTGATATTTATGACTTGCAACTCACGCATTTGATGTCGGATATGGAATTAAGAAGAGTGTTGCTGGCAACAACAAATAAATCCATACTTGTGATTGAGGATATAGATTGTAGTCAATTGAAGCTGCAAGATAGAAAGAACAGCAATAAGAAAAATGACTCAGAAAGCAATCAGCTTTCGCTATTGGGACGACTACTGAACTTCACAGATGGCCTGTGGTATAGTTGTGGAGAAGAGAGAATCATTATATTCACTACCAGTCACAAGAGCAAACTAGACCCTGCATTGCTGCGTTCAGGAAGAATGGACATGCACGTCCACATGTCGTATTGCTCATATGAGGGCTTCAAAACATTGGCCTCAAATTACCTAGGCATCAAGGAACACCAACCTTTGTTTGATGAGATTGAAAGCCTACTGCAGAAGACAGAGGTCACTCCTGCACAAGTGGCCGAGGAGCTGATGAAGAGTGAGATTGCTGATGTTGCACTCCAAGCTTTGATCACAGGGATAAAGGAAATGAATAAAAGGGAGGAAGATGAAAAAAAGGCCAAAGATGAAGAGAAAGTTAAGAACGATAAAGAGAAGGTTGATCATAAACAGTTGACATATTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

445

Amino Acids

51.92

Weight (kDa)

8.78

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 15 - 99 3.6e-15 Domain associated at C-terminal with AAA
AAA PF00004 217 - 348 1e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 350 - 422 2.6e-24 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 674
AcoI YGGCCR 1 cut(s) 1167
AcuI CTGAAG 2 cut(s) 132, 734
AfaI GTAC 2 cut(s) 8, 204
AfiI CCNNNNNNNGG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 1038
AgsI TTSAA 6 cut(s) 104, 260, 430, 838, 1066, 1127
AjiI CACGTC 1 cut(s) 1033
AjnI CCWGG 1 cut(s) 667
Alw21I GWGCWC 2 cut(s) 125, 363
Alw26I GTCTC 4 cut(s) 22, 212, 416, 533
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 4 cut(s) 928, 1074, 1167, 1266
ApeKI GCWGC 4 cut(s) 346, 463, 841, 1006
Asp700I GAANNNNTTC 1 cut(s) 611
AspA2I CCTAGG 1 cut(s) 1087
AspS9I GGNCC 1 cut(s) 662
AsuHPI GGTGA 1 cut(s) 37
AsuII TTCGAA 2 cut(s) 141, 519
AvaII GGWCC 1 cut(s) 662
AvrII CCTAGG 1 cut(s) 1087
BanII GRGCYC 2 cut(s) 179, 363
BbsI GAAGAC 2 cut(s) 267, 1148
Bbv12I GWGCWC 2 cut(s) 125, 363
BbvI GCAGC 4 cut(s) 358, 450, 828, 993
BccI CCATC 3 cut(s) 239, 495, 920
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 3 cut(s) 90, 1218, 1309
BcoDI GTCTC 4 cut(s) 22, 212, 416, 533
BfaI CTAG 4 cut(s) 186, 327, 992, 1088
BfmI CTRYAG 1 cut(s) 1136
BisI GCNGC 4 cut(s) 347, 464, 842, 1007
BlnI CCTAGG 1 cut(s) 1087
BlsI GCNGC 4 cut(s) 348, 465, 843, 1008
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 1033
BmgT120I GGNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 3 cut(s) 388, 433, 1101
BoxI GACNNNNGTC 1 cut(s) 1148
BpiI GAAGAC 2 cut(s) 267, 1148
BpmI CTGGAG 1 cut(s) 651
Bpu14I TTCGAA 2 cut(s) 141, 519
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 5 cut(s) 112, 549, 668, 1087, 1170
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse1I ACTGG 2 cut(s) 679, 975
Bse3DI GCAATG 1 cut(s) 1001
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 5 cut(s) 112, 549, 668, 1087, 1170
BseGI GGATG 4 cut(s) 37, 231, 403, 683
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMI GCAATG 1 cut(s) 1001
BseMII CTCAG 4 cut(s) 224, 376, 549, 891
BseNI ACTGG 2 cut(s) 679, 975
BseRI GAGGAG 2 cut(s) 182, 1187
BseXI GCAGC 4 cut(s) 358, 450, 828, 993
BsgI GTGCAG 1 cut(s) 1143
BshFI GGCC 4 cut(s) 930, 1076, 1169, 1268
BsiHKAI GWGCWC 2 cut(s) 125, 363
BslFI GGGAC 2 cut(s) 685, 918
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 4 cut(s) 22, 212, 416, 533
BsmFI GGGAC 2 cut(s) 685, 918
BsmI GAATGC 2 cut(s) 53, 511
BsnI GGCC 4 cut(s) 930, 1076, 1169, 1268
Bso31I GGTCTC 1 cut(s) 533
Bsp119I TTCGAA 2 cut(s) 141, 519
Bsp1286I GDGCHC 3 cut(s) 125, 179, 363
Bsp1407I TGTACA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 68, 90, 569, 1218, 1309
Bsp19I CCATGG 2 cut(s) 112, 549
BspANI GGCC 4 cut(s) 930, 1076, 1169, 1268
BspCNI CTCAG 4 cut(s) 223, 375, 550, 890
BspHI TCATGA 1 cut(s) 87
BspMAI CTGCAG 1 cut(s) 1140
BspT104I TTCGAA 2 cut(s) 141, 519
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 1001
BsrGI TGTACA 1 cut(s) 202
BsrI ACTGG 2 cut(s) 679, 975
BssECI CCNNGG 5 cut(s) 112, 549, 668, 1087, 1170
BssMI GATC 5 cut(s) 68, 90, 569, 1218, 1309
BssT1I CCWWGG 3 cut(s) 112, 549, 1087
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 1 cut(s) 1320
Bst6I CTCTTC 5 cut(s) 560, 766, 945, 1178, 1272
BstAUI TGTACA 1 cut(s) 202
BstBI TTCGAA 2 cut(s) 141, 519
BstC8I GCNNGC 1 cut(s) 783
BstDEI CTNAG 5 cut(s) 210, 222, 362, 558, 877
BstDSI CCRYGG 2 cut(s) 112, 549
BstF5I GGATG 4 cut(s) 37, 231, 403, 683
BstKTI GATC 5 cut(s) 71, 93, 572, 1221, 1312
BstMAI GTCTC 4 cut(s) 22, 212, 416, 533
BstMBI GATC 5 cut(s) 68, 90, 569, 1218, 1309
BstMWI GCNNNNNNNGC 5 cut(s) 160, 742, 1166, 1202, 1211
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 2 cut(s) 1030, 1042
BstPAI GACNNNNGTC 1 cut(s) 1148
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 1136
BstV1I GCAGC 4 cut(s) 358, 450, 828, 993
BstV2I GAAGAC 2 cut(s) 267, 1148
BstXI CCANNNNNNTGG 1 cut(s) 550
BsuRI GGCC 4 cut(s) 930, 1076, 1169, 1268
BtgI CCRYGG 2 cut(s) 112, 549
BtrI CACGTC 1 cut(s) 1033
BtsCI GGATG 4 cut(s) 37, 231, 403, 683
Cac8I GCNNGC 1 cut(s) 783
CaiI CAGNNNCTG 1 cut(s) 674
CciI TCATGA 1 cut(s) 87
Cfr13I GGNCC 1 cut(s) 662
Csp6I GTAC 2 cut(s) 7, 203
CviAII CATG 9 cut(s) 50, 88, 94, 113, 118, 394, 550, 1027, 1039
CviQI GTAC 2 cut(s) 7, 203
DdeI CTNAG 5 cut(s) 210, 222, 362, 558, 877
DpnI GATC 5 cut(s) 70, 92, 571, 1220, 1311
DpnII GATC 5 cut(s) 68, 90, 569, 1218, 1309
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 1 cut(s) 1167
Eam1104I CTCTTC 5 cut(s) 560, 766, 945, 1178, 1272
EarI CTCTTC 5 cut(s) 560, 766, 945, 1178, 1272
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 3 cut(s) 112, 549, 1087
Eco24I GRGCYC 2 cut(s) 179, 363
Eco31I GGTCTC 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 662
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 2 cut(s) 132, 734
EcoICRI GAGCTC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 3 cut(s) 112, 549, 1087
EcoT22I ATGCAT 1 cut(s) 55
EcoT38I GRGCYC 2 cut(s) 179, 363
ErhI CCWWGG 3 cut(s) 112, 549, 1087
FaeI CATG 9 cut(s) 53, 91, 97, 116, 121, 397, 553, 1030, 1042
FaqI GGGAC 2 cut(s) 685, 918
FatI CATG 9 cut(s) 49, 87, 93, 112, 117, 393, 549, 1026, 1038
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 3 cut(s) 90, 1218, 1309
Fnu4HI GCNGC 4 cut(s) 347, 464, 842, 1007
FokI GGATG 4 cut(s) 24, 218, 410, 670
FriOI GRGCYC 2 cut(s) 179, 363
Fsp4HI GCNGC 4 cut(s) 347, 464, 842, 1007
FspBI CTAG 4 cut(s) 186, 327, 992, 1088
GluI GCNGC 4 cut(s) 347, 464, 842, 1007
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 4 cut(s) 930, 1076, 1169, 1268
Hin1II CATG 9 cut(s) 53, 91, 97, 116, 121, 397, 553, 1030, 1042
HincII GTYRAC 1 cut(s) 1323
HindII GTYRAC 1 cut(s) 1323
HindIII AAGCTT 2 cut(s) 144, 1212
HinfI GANTC 5 cut(s) 189, 276, 554, 875, 957
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 1036, 1323
Hpy188I TCNGA 8 cut(s) 151, 213, 365, 484, 559, 714, 757, 880
Hpy188III TCNNGA 3 cut(s) 88, 538, 1014
Hpy8I GTNNAC 2 cut(s) 1036, 1323
HpyAV CCTTC 4 cut(s) 189, 266, 624, 1297
HpyCH4III ACNGT 1 cut(s) 1320
HpyCH4IV ACGT 1 cut(s) 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 742, 1166, 1202, 1211
HpyF3I CTNAG 5 cut(s) 210, 222, 362, 558, 877
HpySE526I ACGT 1 cut(s) 1032
Hsp92II CATG 9 cut(s) 53, 91, 97, 116, 121, 397, 553, 1030, 1042
Ksp22I TGATCA 3 cut(s) 90, 1218, 1309
Kzo9I GATC 5 cut(s) 68, 90, 569, 1218, 1309
LmnI GCTCC 1 cut(s) 1174
Lsp1109I GCAGC 4 cut(s) 358, 450, 828, 993
LweI GCATC 3 cut(s) 388, 433, 1101
MaeI CTAG 4 cut(s) 186, 327, 992, 1088
MaeII ACGT 1 cut(s) 1032
MaeIII GTNAC 2 cut(s) 977, 1150
MalI GATC 5 cut(s) 70, 92, 571, 1220, 1311
MboI GATC 5 cut(s) 68, 90, 569, 1218, 1309
MfeI CAATTG 1 cut(s) 833
MhlI GDGCHC 3 cut(s) 125, 179, 363
MlyI GAGTC 3 cut(s) 183, 285, 869
MmeI TCCRAC 2 cut(s) 40, 735
Mph1103I ATGCAT 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 4 cut(s) 11, 600, 767, 1287
MslI CAYNNNNRTG 3 cut(s) 92, 548, 806
MspA1I CMGCKG 2 cut(s) 77, 283
MspR9I CCNGG 1 cut(s) 669
MunI CAATTG 1 cut(s) 833
Mva1269I GAATGC 2 cut(s) 53, 511
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 5 cut(s) 160, 742, 1166, 1202, 1211
NcoI CCATGG 2 cut(s) 112, 549
NdeI CATATG 1 cut(s) 1054
NdeII GATC 5 cut(s) 68, 90, 569, 1218, 1309
NlaIII CATG 9 cut(s) 53, 91, 97, 116, 121, 397, 553, 1030, 1042
NmeAIII GCCGAG 1 cut(s) 1195
NmuCI GTSAC 2 cut(s) 977, 1150
NsiI ATGCAT 1 cut(s) 55
NspI RCATGY 2 cut(s) 1030, 1042
NspV TTCGAA 2 cut(s) 141, 519
PagI TCATGA 1 cut(s) 87
PciI ACATGT 1 cut(s) 1038
PctI GAATGC 2 cut(s) 53, 511
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 2 cut(s) 554, 957
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 4 cut(s) 348, 465, 843, 1008
PleI GAGTC 3 cut(s) 183, 284, 869
PpsI GAGTC 3 cut(s) 183, 284, 869
PscI ACATGT 1 cut(s) 1038
PshAI GACNNNNGTC 1 cut(s) 1148
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspPI GGNCC 1 cut(s) 662
PstI CTGCAG 1 cut(s) 1140
PstNI CAGNNNCTG 1 cut(s) 674
PvuII CAGCTG 2 cut(s) 77, 283
RsaI GTAC 2 cut(s) 8, 204
RsaNI GTAC 2 cut(s) 7, 203
RseI CAYNNNNRTG 3 cut(s) 92, 548, 806
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 4 cut(s) 11, 600, 767, 1287
SatI GCNGC 4 cut(s) 347, 464, 842, 1007
Sau3AI GATC 5 cut(s) 68, 90, 569, 1218, 1309
Sau96I GGNCC 1 cut(s) 662
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 3 cut(s) 183, 285, 869
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 3 cut(s) 125, 179, 363
SfaNI GCATC 3 cut(s) 388, 433, 1101
SfcI CTRYAG 1 cut(s) 1136
SfuI TTCGAA 2 cut(s) 141, 519
SinI GGWCC 1 cut(s) 662
SmiMI CAYNNNNRTG 3 cut(s) 92, 548, 806
SspMI CTAG 4 cut(s) 186, 327, 992, 1088
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 3 cut(s) 112, 549, 1087
TaaI ACNGT 1 cut(s) 1320
TaiI ACGT 1 cut(s) 1035
TaqI TCGA 3 cut(s) 141, 519, 537
TatI WGTACW 2 cut(s) 6, 202
TfiI GAWTC 2 cut(s) 554, 957
Tru1I TTAA 4 cut(s) 11, 600, 767, 1287
Tru9I TTAA 4 cut(s) 11, 600, 767, 1287
TseFI GTSAC 2 cut(s) 977, 1150
TseI GCWGC 4 cut(s) 346, 463, 841, 1006
Tsp45I GTSAC 2 cut(s) 977, 1150
TspDTI ATGAA 8 cut(s) 26, 96, 285, 452, 1196, 1253, 1272, 1290
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 662
XceI RCATGY 2 cut(s) 1030, 1042
XmaJI CCTAGG 1 cut(s) 1087
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 4 cut(s) 186, 327, 992, 1088
ZrmI AGTACT 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.