Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007335

Physical Location & Seq

Genomic Coordinates Forward (+)

38357940 .. 38360075

Gene Structure 2136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1251800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGTTTCTCTGCGAATTCTGAGAACCCAAGAACTCCAACTGAAGCTATTATCCCCTGCTTCAACCCCCATTTCTCTATTCTACACCCTCTTCTCTCTCAGAACACTCTCTTCTTACAGTCACTATGACGACCCAAACTCTGCAACCACCACCGCCATCGCCGCCGCCGCCGCTTCTTCTCAATCGCAGTCACTTGTCAGCTCGATCTGCTCATTGGTATACCAATCCTATTCCCCGCAAACCCATCTGAGATCTTCGCCTCCGAAGCTCAATCTCGACTTAAATCCTGAATCTTTGACCCACGAGCAGGCCATTTCCGTCGTTGCTTCACTGGCTCAGGAAGCAGGTTCCATGGCGGCGCTGAGTTTCTTTTACTGGGCAATTGGGTTTCCCAAGTTTCGATATTTCATGCGGCTTTACATATTCTGCGCAATGGCGATACTTCGCAATGGGAATCTGGAGAGAGCCCATGAGGTGGTTCATTGTATGGTGATGAATTTCGCTGAGATTGAGAGGTTGAAGGAGGCTGCTGACATGGTCTTTGAGATACAGAATCAGGGTCTTGCGTTGAGTTCTCGGACGTTGAATTGTGTTCTTGGGATTGCTTGCGATTTGGGTTTGGTTGAGTATGCAGAGAATCTGTTCGAGGAAATGTGTGATAGAGGTGTGTCTCCTGATTCCTTGAGCTATAAGTCTATGGTTGTTGGGTTTTGTAGGAACGGGAGGGTTTCAGACGCGGATAGGTGGTTGGGTAAGATGCTCGAGAGAGGTTTTGTTTTGGATAATGCATCGTTTACTTTGATCATAAACATGTTTTGCGAGAAGGGTCTTGTAGGTCGAGCGTCTTGGTGTTTTGATAAGATGATTAGGATGGGTGTGAAGCCGAATTTGATTAATTTCACGACGATGATTAATGGGTTGTGCAAGAGGGGCAGCATTAAACAAGCATTTGAAATGTTGGAGGAAATGGTGAGGAAAGGTTGGAAGCCGAATGTGTATACACACACAGCATTGATTGATGGGCTCTGCAAGAAAGGCTGGACTGAAAGGGCGTTTAGACTGTTTCTTAAGCTTGTCCGGAGCGATAATCACAAGCCAAATGTACATACATATACTGCTATGATTAATGGATATTGCCAAGAGGACAAAATGAGCCGCGCGGAGATGTTATTGAGCAGGATGAAAGAACAAGGATTAGTTCCTAACACCAACACGTATACTTCTCTAGTTTCAGGGCATTGTAAAGCTGGGAACTTTGAAAGAGCATATGAGTTGATGGATATAATGGATAAAGAAGGATTCCCTCCTAATATCTATACATACAATGCAGTAATTGACAGTCTCTGTAAAAGGGGGAGGGTTGAAGAAGCAAATAAACTGATTAGGAAGGGCTTTCGCCGAGGATTGGCAGCTGATAGGGTCACGTATACAATTTTCATATCTGAGCACTGCAAACGGGCTGATATTAATGGGGCCCTGGTGTTTTTTACTAAGATGGTGAAGGTTGGATTGCAGCCTGATATACATTTGTATACAACTTTGATTGCTGCCTTCTGCAGGCAAAAGAAAATGAAAGAAAGCGAGAAACTTCTTGAGTTTGTTGTCAGGGCTGACTTGATTCCCACCAAGGAAACCTATACATCCATGATTTGTGGGCATTGTCGGGATGGAAATATTGCTTCTGCTATTAAGTATTTCCAAAGGATGGGTGACCATGGTTGTGCGCCTGATAGTATTACTTACGGTGCTCTGATAAGTGGGCTCTGTAAGGAGGAGAAATTGGAAGAGGCTCGTAGGTTATATGATACCATGATGGACAAAGGGCTATCGCCCTGTGAAGTCACTAGATTTACATTAGCTTACAAGTACTGCATGAAAGATGACTCTGCCGCTGCTATGGTTATGTTGGAAAGGCTAGAAAAGAAGCTCTGGATCCGCACAGTGAATACATTGGTCAGGAAGCTTTGTAGTGAGAAGAAAGTAGGGATAGCAGCATTGTTCTTTCATAAATTAGTAGACAAGGACCAAAACGTGGACCGTGTGACATTGGCAGCGTTTATGACAGCATGCTATGAGAGCAACAAGTATGCTCTCGTTTCAGATTTGACTGAGAGGATCTCTAAAGGAATTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

712

Amino Acids

80.33

Weight (kDa)

8.86

Isoelectric Point (pI)

35.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	193 - 239	1.9e-09	PPR repeat family
PPR_1	PF12854	221 - 250	1e-05	PPR repeat
PPR_2	PF13041	229 - 273	3.6e-07	PPR repeat family
PPR_2	PF13041	263 - 309	9.1e-10	PPR repeat family
PPR_3	PF13812	285 - 340	4.2e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	291 - 324	4.3e-12	PPR repeat
PPR_2	PF13041	295 - 344	3e-18	PPR repeat family
PPR	PF01535	300 - 327	3.8e-07	PPR repeat
PPR_3	PF13812	319 - 378	1.5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	326 - 355	1.6e-09	PPR repeat
PPR_2	PF13041	336 - 380	6.5e-11	PPR repeat family
TPR_24	PF23276	357 - 459	2.1e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	360 - 406	7.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 395	1.7e-09	PPR repeat
PPR_2	PF13041	366 - 415	1.3e-16	PPR repeat family
PPR	PF01535	370 - 399	5.5e-08	PPR repeat
PPR_long	PF17177	389 - 458	3.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	390 - 447	7.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	398 - 429	2.7e-10	PPR repeat
PPR	PF01535	405 - 434	1.2e-06	PPR repeat
PPR_2	PF13041	405 - 450	1.6e-14	PPR repeat family
PPR_1	PF12854	433 - 462	7.2e-10	PPR repeat
PPR_2	PF13041	436 - 485	9.9e-13	PPR repeat family
PPR_2	PF13041	472 - 520	9.5e-11	PPR repeat family
PPR_1	PF12854	503 - 532	9.5e-06	PPR repeat
PPR_2	PF13041	506 - 555	1.7e-09	PPR repeat family
PPR_1	PF12854	539 - 569	1.6e-06	PPR repeat
PPR_2	PF13041	544 - 590	6.1e-14	PPR repeat family
PPR	PF01535	545 - 574	1.6e-06	PPR repeat
PPR_1	PF12854	573 - 604	1.8e-10	PPR repeat
PPR	PF01535	579 - 609	9.8e-07	PPR repeat
PPR_2	PF13041	581 - 615	2e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19890
fragaria_vesca	FvH4_3g12270 FvH4_3g12270 FvH4_3g12270
malus_domestica	MD05G1251800.v1.1
prunus_persica	Prupe.4G111800_v2.0.a1
pyrus_communis	pycom05g22980 pycom10g19560
rosa_chinensis	RchiOBHm_Chr5g0020081 RchiOBHm_Chr7g0211421
rosa_laevigata	RLG00000032497
rosa_multiflora	Rmu_sc0021557.1_g000003
rosa_roxburghii	Rroxscaffold_1G00058120
rosa_rugosa	Rorug05G0056700
rosa_samantha	Rh5AG146800 Rh5BG146300 Rh5CG157900 Rh5DG145800 Rh6CG070300 Rh6DG067500 Rh7AG292200 Rh7CG312200 Rh7DG257000 Rh7DG263700
rosa_wichuraiana	Rw5G012970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	430
Acc36I	ACCTGC	1 cut(s)	335
AccB7I	CCANNNNNTGG	3 cut(s)	475, 1705, 2032
AccI	GTMKAC	6 cut(s)	219, 998, 1217, 1427, 1532, 2016
AccII	CGCG	3 cut(s)	737, 1158, 1160
AccIII	TCCGGA	1 cut(s)	1077
AclWI	GGATC	3 cut(s)	1927, 1940, 2123
AcsI	RAATTY	3 cut(s)	15, 496, 886
AcuI	CTGAAG	1 cut(s)	62
AfaI	GTAC	2 cut(s)	1104, 1868
AfiI	CCNNNNNNNGG	6 cut(s)	307, 475, 1405, 1557, 1705, 2032
AflII	CTTAAG	1 cut(s)	1067
AflIII	ACRYGT	2 cut(s)	810, 1212
AgsI	TTSAA	6 cut(s)	63, 520, 586, 953, 1259, 1364
AjnI	CCWGG	1 cut(s)	1476
Alw21I	GWGCWC	2 cut(s)	1449, 1750
Alw26I	GTCTC	2 cut(s)	675, 1346
AlwI	GGATC	3 cut(s)	1927, 1940, 2123
AlwNI	CAGNNNCTG	1 cut(s)	1344
Ama87I	CYCGRG	1 cut(s)	761
Aor13HI	TCCGGA	1 cut(s)	1077
AoxI	GGCC	2 cut(s)	309, 1473
ApaI	GGGCCC	1 cut(s)	1477
ApeKI	GCWGC	8 cut(s)	527, 933, 1409, 1513, 1547, 1892, 1991, 2051
ApoI	RAATTY	3 cut(s)	15, 496, 886
ArsI	GACNNNNNNTTYG	2 cut(s)	524, 556
AseI	ATTAAT	4 cut(s)	894, 912, 1125, 1467
Asp700I	GAANNNNTTC	1 cut(s)	641
AspLEI	GCGC	4 cut(s)	361, 431, 1160, 1726
AspS9I	GGNCC	4 cut(s)	1473, 1474, 2023, 2035
AsuHPI	GGTGA	4 cut(s)	502, 982, 1510, 1721
AvaI	CYCGRG	1 cut(s)	761
AvaII	GGWCC	2 cut(s)	2023, 2035
BaeGI	GKGCMC	1 cut(s)	1477
BamHI	GGATCC	1 cut(s)	1932
BanII	GRGCYC	4 cut(s)	469, 1026, 1477, 1764
BauI	CACGAG	1 cut(s)	302
Bbv12I	GWGCWC	2 cut(s)	1449, 1750
BbvI	GCAGC	8 cut(s)	514, 945, 1421, 1525, 1534, 1879, 2003, 2063
BccI	CCATC	9 cut(s)	164, 252, 865, 1013, 1270, 1489, 1661, 1699, 1807
BciT130I	CCWGG	1 cut(s)	1478
BclI	TGATCA	1 cut(s)	801
BcoDI	GTCTC	2 cut(s)	675, 1346
BfaI	CTAG	4 cut(s)	1226, 1845, 1916, 2134
BfmI	CTRYAG	1 cut(s)	1555
BfoI	RGCGCY	1 cut(s)	362
BfrI	CTTAAG	1 cut(s)	1067
BfuAI	ACCTGC	1 cut(s)	335
BglII	AGATCT	1 cut(s)	251
BmcAI	AGTACT	1 cut(s)	1868
Bme1390I	CCNGG	1 cut(s)	1478
Bme18I	GGWCC	2 cut(s)	2023, 2035
BmeT110I	CYCGRG	1 cut(s)	761
BmgT120I	GGNCC	4 cut(s)	1473, 1474, 2023, 2035
BmiI	GGNNCC	4 cut(s)	349, 1474, 1475, 1934
BmrFI	CCNGG	1 cut(s)	1478
BmrI	ACTGGG	1 cut(s)	385
BmsI	GCATC	2 cut(s)	747, 797
BmuI	ACTGGG	1 cut(s)	385
BplI	GAGNNNNNCTC	2 cut(s)	2102, 2134
BpmI	CTGGAG	1 cut(s)	479
Bpu10I	CCTNAGC	1 cut(s)	336
BpuEI	CTTGAG	2 cut(s)	703, 1613
BsaAI	YACGTR	2 cut(s)	1215, 1425
BsaJI	CCNNGG	5 cut(s)	351, 1399, 1476, 1626, 1714
BsaWI	WCCGGW	1 cut(s)	1077
Bsc4I	CCNNNNNNNGG	6 cut(s)	307, 475, 1405, 1557, 1705, 2032
Bse1I	ACTGG	2 cut(s)	336, 380
Bse3DI	GCAATG	2 cut(s)	438, 454
BseAI	TCCGGA	1 cut(s)	1077
BseBI	CCWGG	1 cut(s)	1478
BseDI	CCNNGG	5 cut(s)	351, 1399, 1476, 1626, 1714
BseGI	GGATG	5 cut(s)	876, 1185, 1640, 1672, 1710
BseLI	CCNNNNNNNGG	6 cut(s)	307, 475, 1405, 1557, 1705, 2032
BseMI	GCAATG	2 cut(s)	438, 454
BseMII	CTCAG	8 cut(s)	11, 112, 239, 350, 353, 495, 1434, 2100
BseNI	ACTGG	2 cut(s)	336, 380
BseRI	GAGGAG	1 cut(s)	1787
BseSI	GKGCMC	1 cut(s)	1477
BseXI	GCAGC	8 cut(s)	514, 945, 1421, 1525, 1534, 1879, 2003, 2063
BseYI	CCCAGC	1 cut(s)	1247
Bsh1236I	CGCG	3 cut(s)	737, 1158, 1160
BshFI	GGCC	2 cut(s)	311, 1475
BsiHKAI	GWGCWC	2 cut(s)	1449, 1750
BsiHKCI	CYCGRG	1 cut(s)	761
BsiSI	CCGG	1 cut(s)	1078
BslI	CCNNNNNNNGG	6 cut(s)	307, 475, 1405, 1557, 1705, 2032
BsmAI	GTCTC	2 cut(s)	675, 1346
BsnI	GGCC	2 cut(s)	311, 1475
BsoBI	CYCGRG	1 cut(s)	761
Bsp120I	GGGCCC	1 cut(s)	1473
Bsp1286I	GDGCHC	6 cut(s)	469, 1026, 1449, 1477, 1750, 1764
Bsp13I	TCCGGA	1 cut(s)	1077
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp143I	GATC	5 cut(s)	204, 251, 801, 1932, 2115
Bsp19I	CCATGG	2 cut(s)	351, 1714
BspANI	GGCC	2 cut(s)	311, 1475
BspCNI	CTCAG	8 cut(s)	12, 111, 240, 349, 354, 496, 1435, 2101
BspEI	TCCGGA	1 cut(s)	1077
BspFNI	CGCG	3 cut(s)	737, 1158, 1160
BspLI	GGNNCC	4 cut(s)	349, 1474, 1475, 1934
BspMAI	CTGCAG	1 cut(s)	1559
BspMI	ACCTGC	1 cut(s)	335
BspPI	GGATC	3 cut(s)	1927, 1940, 2123
BspTI	CTTAAG	1 cut(s)	1067
BsrDI	GCAATG	2 cut(s)	438, 454
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	2 cut(s)	336, 380
BssECI	CCNNGG	5 cut(s)	351, 1399, 1476, 1626, 1714
BssMI	GATC	5 cut(s)	204, 251, 801, 1932, 2115
BssNAI	GTATAC	5 cut(s)	220, 999, 1218, 1428, 1533
BssSI	CACGAG	1 cut(s)	302
BssT1I	CCWWGG	3 cut(s)	351, 1626, 1714
Bst1107I	GTATAC	5 cut(s)	220, 999, 1218, 1428, 1533
Bst2BI	CACGAG	1 cut(s)	302
Bst2UI	CCWGG	1 cut(s)	1478
Bst4CI	ACNGT	6 cut(s)	119, 1062, 1340, 1745, 1942, 2039
Bst6I	CTCTTC	3 cut(s)	95, 115, 1779
BstAFI	CTTAAG	1 cut(s)	1067
BstAUI	TGTACA	1 cut(s)	1102
BstBAI	YACGTR	2 cut(s)	1215, 1425
BstC8I	GCNNGC	4 cut(s)	309, 607, 1559, 2068
BstDEI	CTNAG	9 cut(s)	20, 98, 248, 336, 362, 504, 1443, 1491, 2109
BstDSI	CCRYGG	2 cut(s)	351, 1714
BstEII	GGTNACC	1 cut(s)	1709
BstENI	CCTNNNNNAGG	1 cut(s)	1555
BstF5I	GGATG	5 cut(s)	876, 1185, 1640, 1672, 1710
BstFNI	CGCG	3 cut(s)	737, 1158, 1160
BstH2I	RGCGCY	1 cut(s)	362
BstHHI	GCGC	4 cut(s)	361, 431, 1160, 1726
BstKTI	GATC	5 cut(s)	207, 254, 804, 1935, 2118
BstMAI	GTCTC	2 cut(s)	675, 1346
BstMBI	GATC	5 cut(s)	204, 251, 801, 1932, 2115
BstNI	CCWGG	1 cut(s)	1478
BstNSI	RCATGY	2 cut(s)	814, 2070
BstPI	GGTNACC	1 cut(s)	1709
BstSCI	CCNGG	1 cut(s)	1476
BstSFI	CTRYAG	1 cut(s)	1555
BstSLI	GKGCMC	1 cut(s)	1477
BstUI	CGCG	3 cut(s)	737, 1158, 1160
BstV1I	GCAGC	8 cut(s)	514, 945, 1421, 1525, 1534, 1879, 2003, 2063
BstX2I	RGATCY	3 cut(s)	251, 1932, 2115
BstYI	RGATCY	3 cut(s)	251, 1932, 2115
BstZ17I	GTATAC	5 cut(s)	220, 999, 1218, 1428, 1533
BsuRI	GGCC	2 cut(s)	311, 1475
BtgI	CCRYGG	2 cut(s)	351, 1714
BtgZI	GCGATG	1 cut(s)	142
BtsCI	GGATG	5 cut(s)	876, 1185, 1640, 1672, 1710
BtsI	GCAGTG	1 cut(s)	1447
BtsIMutI	CAGTG	3 cut(s)	329, 1447, 1947
BveI	ACCTGC	1 cut(s)	335
Cac8I	GCNNGC	4 cut(s)	309, 607, 1559, 2068
CaiI	CAGNNNCTG	1 cut(s)	1344
CfoI	GCGC	4 cut(s)	361, 431, 1160, 1726
Cfr13I	GGNCC	4 cut(s)	1473, 1474, 2023, 2035
CseI	GACGC	2 cut(s)	743, 831
Csp6I	GTAC	2 cut(s)	1103, 1867
CviQI	GTAC	2 cut(s)	1103, 1867
DdeI	CTNAG	9 cut(s)	20, 98, 248, 336, 362, 504, 1443, 1491, 2109
DpnI	GATC	5 cut(s)	206, 253, 803, 1934, 2117
DpnII	GATC	5 cut(s)	204, 251, 801, 1932, 2115
Eam1104I	CTCTTC	3 cut(s)	95, 115, 1779
EarI	CTCTTC	3 cut(s)	95, 115, 1779
Eco130I	CCWWGG	3 cut(s)	351, 1626, 1714
Eco24I	GRGCYC	4 cut(s)	469, 1026, 1477, 1764
Eco47I	GGWCC	2 cut(s)	2023, 2035
Eco57I	CTGAAG	1 cut(s)	62
Eco88I	CYCGRG	1 cut(s)	761
Eco91I	GGTNACC	1 cut(s)	1709
EcoNI	CCTNNNNNAGG	1 cut(s)	1555
EcoO109I	RGGNCCY	2 cut(s)	1473, 1474
EcoO65I	GGTNACC	1 cut(s)	1709
EcoRI	GAATTC	1 cut(s)	15
EcoRII	CCWGG	1 cut(s)	1476
EcoT14I	CCWWGG	3 cut(s)	351, 1626, 1714
EcoT22I	ATGCAT	1 cut(s)	790
EcoT38I	GRGCYC	4 cut(s)	469, 1026, 1477, 1764
ErhI	CCWWGG	3 cut(s)	351, 1626, 1714
FauI	CCCGC	1 cut(s)	243
FauNDI	CATATG	1 cut(s)	1267
FbaI	TGATCA	1 cut(s)	801
FblI	GTMKAC	6 cut(s)	219, 998, 1217, 1427, 1532, 2016
FokI	GGATG	5 cut(s)	883, 1192, 1627, 1679, 1717
FriOI	GRGCYC	4 cut(s)	469, 1026, 1477, 1764
FspBI	CTAG	4 cut(s)	1226, 1845, 1916, 2134
FspI	TGCGCA	1 cut(s)	430
GlaI	GCGC	4 cut(s)	360, 430, 1159, 1725
GsaI	CCCAGC	1 cut(s)	1251
GsuI	CTGGAG	1 cut(s)	479
HaeII	RGCGCY	1 cut(s)	362
HaeIII	GGCC	2 cut(s)	311, 1475
HapII	CCGG	1 cut(s)	1078
HgaI	GACGC	2 cut(s)	743, 831
HhaI	GCGC	4 cut(s)	361, 431, 1160, 1726
Hin6I	GCGC	4 cut(s)	359, 429, 1158, 1724
HinP1I	GCGC	4 cut(s)	359, 429, 1158, 1724
HindIII	AAGCTT	2 cut(s)	1070, 1961
HinfI	GANTC	8 cut(s)	290, 454, 553, 637, 677, 1299, 1618, 1883
HpaII	CCGG	1 cut(s)	1078
HphI	GGTGA	4 cut(s)	502, 982, 1510, 1721
Hpy166II	GTNNAC	8 cut(s)	220, 795, 999, 1218, 1428, 1533, 2017, 2035
Hpy188I	TCNGA	9 cut(s)	21, 101, 249, 264, 579, 733, 1444, 1752, 2101
Hpy8I	GTNNAC	8 cut(s)	220, 795, 999, 1218, 1428, 1533, 2017, 2035
Hpy99I	CGWCG	2 cut(s)	323, 907
HpyAV	CCTTC	6 cut(s)	514, 817, 1289, 1381, 1495, 1561
HpyCH4III	ACNGT	6 cut(s)	119, 1062, 1340, 1745, 1942, 2039
HpyCH4IV	ACGT	4 cut(s)	581, 1214, 1424, 2031
HpyF3I	CTNAG	9 cut(s)	20, 98, 248, 336, 362, 504, 1443, 1491, 2109
HpySE526I	ACGT	4 cut(s)	581, 1214, 1424, 2031
HspAI	GCGC	4 cut(s)	359, 429, 1158, 1724
Kpn2I	TCCGGA	1 cut(s)	1077
Ksp22I	TGATCA	1 cut(s)	801
Kzo9I	GATC	5 cut(s)	204, 251, 801, 1932, 2115
LmnI	GCTCC	1 cut(s)	1080
Lsp1109I	GCAGC	8 cut(s)	514, 945, 1421, 1525, 1534, 1879, 2003, 2063
LweI	GCATC	2 cut(s)	747, 797
MaeI	CTAG	4 cut(s)	1226, 1845, 1916, 2134
MaeII	ACGT	4 cut(s)	581, 1214, 1424, 2031
MaeIII	GTNAC	6 cut(s)	119, 189, 1420, 1709, 1840, 2041
MalI	GATC	5 cut(s)	206, 253, 803, 1934, 2117
MboI	GATC	5 cut(s)	204, 251, 801, 1932, 2115
MboII	GAAGA	7 cut(s)	82, 102, 168, 246, 1376, 1796, 1987
MfeI	CAATTG	1 cut(s)	381
MflI	RGATCY	3 cut(s)	251, 1932, 2115
MhlI	GDGCHC	6 cut(s)	469, 1026, 1449, 1477, 1750, 1764
MlyI	GAGTC	1 cut(s)	1877
MmeI	TCCRAC	5 cut(s)	61, 939, 962, 1486, 1887
Mph1103I	ATGCAT	1 cut(s)	790
MroI	TCCGGA	1 cut(s)	1077
MroXI	GAANNNNTTC	1 cut(s)	641
MseI	TTAA	8 cut(s)	281, 894, 912, 939, 1068, 1125, 1467, 1689
MslI	CAYNNNNRTG	3 cut(s)	809, 905, 1719
MspA1I	CMGCKG	2 cut(s)	1412, 1892
MspCI	CTTAAG	1 cut(s)	1067
MspI	CCGG	1 cut(s)	1078
MspR9I	CCNGG	1 cut(s)	1478
MunI	CAATTG	1 cut(s)	381
MvaI	CCWGG	1 cut(s)	1478
MvnI	CGCG	3 cut(s)	737, 1158, 1160
NcoI	CCATGG	2 cut(s)	351, 1714
NdeI	CATATG	1 cut(s)	1267
NdeII	GATC	5 cut(s)	204, 251, 801, 1932, 2115
NlaIV	GGNNCC	4 cut(s)	349, 1474, 1475, 1934
NmeAIII	GCCGAG	1 cut(s)	1424
NmuCI	GTSAC	6 cut(s)	119, 189, 1420, 1709, 1840, 2041
NsbI	TGCGCA	1 cut(s)	430
NsiI	ATGCAT	1 cut(s)	790
NspI	RCATGY	2 cut(s)	814, 2070
PaeI	GCATGC	1 cut(s)	2070
PaeR7I	CTCGAG	1 cut(s)	761
PciI	ACATGT	1 cut(s)	810
PdmI	GAANNNNTTC	1 cut(s)	641
PfeI	GAWTC	7 cut(s)	290, 454, 553, 637, 677, 1299, 1618
PflFI	GACNNNGTC	1 cut(s)	536
PflMI	CCANNNNNTGG	3 cut(s)	475, 1705, 2032
PleI	GAGTC	1 cut(s)	1877
PpsI	GAGTC	1 cut(s)	1877
Ppu21I	YACGTR	2 cut(s)	1215, 1425
PscI	ACATGT	1 cut(s)	810
PshBI	ATTAAT	4 cut(s)	894, 912, 1125, 1467
Psp6I	CCWGG	1 cut(s)	1476
PspEI	GGTNACC	1 cut(s)	1709
PspFI	CCCAGC	1 cut(s)	1247
PspGI	CCWGG	1 cut(s)	1476
PspN4I	GGNNCC	4 cut(s)	349, 1474, 1475, 1934
PspOMI	GGGCCC	1 cut(s)	1473
PspPI	GGNCC	4 cut(s)	1473, 1474, 2023, 2035
PstI	CTGCAG	1 cut(s)	1559
PstNI	CAGNNNCTG	1 cut(s)	1344
PsuI	RGATCY	3 cut(s)	251, 1932, 2115
PsyI	GACNNNGTC	1 cut(s)	536
PvuII	CAGCTG	1 cut(s)	1412
RsaI	GTAC	2 cut(s)	1104, 1868
RsaNI	GTAC	2 cut(s)	1103, 1867
RseI	CAYNNNNRTG	3 cut(s)	809, 905, 1719
SaqAI	TTAA	8 cut(s)	281, 894, 912, 939, 1068, 1125, 1467, 1689
Sau3AI	GATC	5 cut(s)	204, 251, 801, 1932, 2115
Sau96I	GGNCC	4 cut(s)	1473, 1474, 2023, 2035
ScaI	AGTACT	1 cut(s)	1868
SchI	GAGTC	1 cut(s)	1877
ScrFI	CCNGG	1 cut(s)	1478
SduI	GDGCHC	6 cut(s)	469, 1026, 1449, 1477, 1750, 1764
SfaNI	GCATC	2 cut(s)	747, 797
SfcI	CTRYAG	1 cut(s)	1555
Sfr274I	CTCGAG	1 cut(s)	761
SinI	GGWCC	2 cut(s)	2023, 2035
SlaI	CTCGAG	1 cut(s)	761
SmiMI	CAYNNNNRTG	3 cut(s)	809, 905, 1719
SmlI	CTYRAG	4 cut(s)	682, 761, 1067, 1592
SmoI	CTYRAG	4 cut(s)	682, 761, 1067, 1592
SphI	GCATGC	1 cut(s)	2070
SspI	AATATT	1 cut(s)	1675
SspMI	CTAG	4 cut(s)	1226, 1845, 1916, 2134
StyD4I	CCNGG	1 cut(s)	1476
StyI	CCWWGG	3 cut(s)	351, 1626, 1714
TaaI	ACNGT	6 cut(s)	119, 1062, 1340, 1745, 1942, 2039
TaiI	ACGT	4 cut(s)	584, 1217, 1427, 2034
TaqI	TCGA	6 cut(s)	203, 276, 400, 645, 762, 838
TatI	WGTACW	2 cut(s)	1102, 1866
TauI	GCSGC	8 cut(s)	164, 167, 170, 173, 359, 415, 1158, 1892
TfiI	GAWTC	7 cut(s)	290, 454, 553, 637, 677, 1299, 1618
Tru1I	TTAA	8 cut(s)	281, 894, 912, 939, 1068, 1125, 1467, 1689
Tru9I	TTAA	8 cut(s)	281, 894, 912, 939, 1068, 1125, 1467, 1689
TscAI	CASTG	3 cut(s)	336, 1454, 1947
TseFI	GTSAC	6 cut(s)	119, 189, 1420, 1709, 1840, 2041
TseI	GCWGC	8 cut(s)	527, 933, 1409, 1513, 1547, 1892, 1991, 2051
Tsp45I	GTSAC	6 cut(s)	119, 189, 1420, 1709, 1840, 2041
TspDTI	ATGAA	8 cut(s)	397, 470, 509, 1196, 1426, 1586, 1889, 1994
TspGWI	ACGGA	1 cut(s)	307
TspRI	CASTG	3 cut(s)	336, 1454, 1947
Tth111I	GACNNNGTC	1 cut(s)	536
Van91I	CCANNNNNTGG	3 cut(s)	475, 1705, 2032
Vha464I	CTTAAG	1 cut(s)	1067
VpaK11BI	GGWCC	2 cut(s)	2023, 2035
VspI	ATTAAT	4 cut(s)	894, 912, 1125, 1467
XagI	CCTNNNNNAGG	1 cut(s)	1555
XapI	RAATTY	3 cut(s)	15, 496, 886
XceI	RCATGY	2 cut(s)	814, 2070
XhoI	CTCGAG	1 cut(s)	761
XmiI	GTMKAC	6 cut(s)	219, 998, 1217, 1427, 1532, 2016
XmnI	GAANNNNTTC	1 cut(s)	641
XspI	CTAG	4 cut(s)	1226, 1845, 1916, 2134
ZrmI	AGTACT	1 cut(s)	1868
Zsp2I	ATGCAT	1 cut(s)	790

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.