Rh5BG146300

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007335
Physical Location & Seq
Reverse (-)
15094909 .. 15099704
4796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG146300.1

Sequence Viewer

Length: 2376 bp
ATGGTGGTTTCTCTTCGAATTCTCAGAAACCATGGACCTCAACACAAGCTATTATCGCCTACATCCACCCACATTTCCCAACTCTACACCCTCTTCTCTCTCAGAACCCTCTCTTCCTTCACTGACTCCGATGAACCATACTCCTCCGCCGCCGCCACAACCACCACCGCCACCACCTCCGATTCCCAATCAGAATCACACTCTCTGGTCACCACAATCTGTTCAATTGTATACAAATCCTACTCCCCACAAACCCATTTTAAATCTTCACCTCCAAATCTCAATCTTGATCTAAACCCCGATTCTTTGACCCACGAACACGCCATTTCCGTCGTGGCTTCGCTGGCCGACGAGGCCGGGTCCATGGTGGCCCTCAGCTTCTTCTACTGGGCAATTGGGTTTGCGAAATTTCGATACTTTATGCGGCTTTACATCTTCTGCGCCGTGTCTATTTTCGGCAATGGCAATTTGGAGAGGACCCATGAAGTGGTTCAGTGTATGGTGAGGAGTTTCGCTGAGATTGGGAGGTTTAAAGAGGCTGCCGATATGGTTTTCGAGATGCAGAACCAGGGCCTTGTGTTGAGTACTCGCACTTTGAACTGTGTTATCGGCATTGCTTGCGAAATGGGTTTAATGGAATATGCAGAGAACGTGTTCGACGAAATGTCTGTGAGAGGAGTGTGTCCTGATGGTTTGAGCTTTAAGTGTATGGTTGTTGGGCAAAAGAAAATGAAAGAAAGTGAGAAACTTTTCGAGGTAGCTGTAAGGCTTGGCTTGATTCCGGCCAAGGAGACCTATACATCCATGATATGTGGGTACTGTAGGGATGGAAATATTGCTTTGGCTGTAAAGTTCTTCCGCAGGATGAGTGATCATGGTTGTTCCCCGGATAGTTTTACTTATGGTGCTCTTATTAGTGGGCTCTGCAAGGAGGAGAAACTTGACGAGGCTCGTAAGTTATATGATACCATGATGGAGAAGGGGCTGTCACCCTGTGAAGTTACTAGGTTGACATTAGCTCACGAGTATTGCCAAAAAGATGACTATGCCACTGCTATGGTCATCTTAGATAGGCTAGAAAAGAAGTTCTGGATCCGCACGGTTAACACATTGGTCAGGAAGCTTTGTTGTGAGAAGAAAGTGGGAATAGCAGCATTGTTCTTTCACAAATTAGTTGATAAGGATCAGAATGTGGATCGTGTGACATTGCAAGCATTTATGACTGCATGTTATGAGAGCAACAAGAACTGGGTCCCTAGGAAGTGCTATAGTGCTAGACTGACGAAACATAATGATGATGATCATTGTTCTGTCTTAAACACTTTGCACCAAGAACTTCTCCCTTACTCTTCCTCAAGCTCAAGCTCAGGCCCAGATTTTCCTTCAGCACACTATCACATGTCTCGGTCTGCACTGGATGAGGTCTCACAATCTGGTGCATTTGTGAGAAGTGCTTCTGAATTCCGTAACTTCATTTCACGAGACCCGAATTCGCAATTTCCAGCAGAAGCTGGGAGATATCATCTTTATATATCTTATGCTTGCCCTTGGGCTTCAAGATGCCTTGCATACTTGAATATTAAAGGACTTCAGAAAGCTATCAGCTTTACGTCGGTCAAACCTATATGGGAAAGAACAAAAGAAAGTGATGAGCATATGGGATGGGTTTTTCCTGCTTCAGATAAGGAGCTAGAAGGAGCTGAACCTGACCCTTTGAATGGAGCAAAAAGTATACGAGAACTTTATGAGATTGCTAGTACAAAATACAGTGGAAAGTACACTGTTCCGGTTCTATGGGATAAAAAACTTGGAACAATTGTCAGTAATGAGAGTTCAGAGATTATTCGCATGTTTAATACTGAATTCAACAGTATAGCAGAGAACCCGGCTTTGGACTTGTATCCCGATCACTTGCAATCCCAAGTCAATCAGACTAATGAATGGATATACGACAAGATAAATAACGGTGTCTATAAATGTGGGTTTGCCAAGAAGCAGGAGCCTTACGATGAGGCTGTGAAACAGTTGTATGAAGCTTTGGACAGATGTGAAGAGATACTTAGCAAGCAACGATACCTCTGTGGAAACACATTATCTGAAGCAGATATTCGTTTGTTTGTGACTCTCATAAGATTTGATGAGGTTTATGCAGTCCACTTCAAGTGCAACAAGAAACTCCTGCGAGAGTACCCAAATCTGTTTAACTACACCAAGGACATTTTCCAAGTTCCTGGAATGAGCAGCACAGTCCAGATGGATCACATCAAACGACACTACTATGGAAGCCATCCTTCTATCAATCCTTTCGGGATAGTTCCTTCCGGCCCAAACATCGACTACTCTTCTCCTCATGACAGAAACAAGTTTTCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

791

Amino Acids

89.65

Weight (kDa)

6.57

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 196 - 238 1e-05 PPR repeat family
PPR_1 PF12854 260 - 290 2.9e-08 PPR repeat
PPR_2 PF13041 265 - 310 5.3e-16 PPR repeat family
PPR PF01535 265 - 294 1.7e-07 PPR repeat
PPR_1 PF12854 293 - 324 1.1e-11 PPR repeat
PPR PF01535 299 - 329 2.7e-07 PPR repeat
PPR_2 PF13041 301 - 344 1.9e-07 PPR repeat family
GST_N_2 PF13409 514 - 620 1.7e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 662 - 742 8.3e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 668 - 737 5.9e-14 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 671 - 745 1.2e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 2 cut(s) 231, 1732
AciI CCGC 7 cut(s) 147, 150, 153, 168, 424, 859, 1096
AclWI GGATC 5 cut(s) 1087, 1100, 1191, 1203, 2265
AcoI YGGCCR 2 cut(s) 345, 783
AcsI RAATTY 5 cut(s) 18, 407, 1460, 1489, 1862
AcuI CTGAAG 4 cut(s) 1368, 1574, 1662, 2118
AdeI CACNNNGTG 1 cut(s) 995
AfaI GTAC 5 cut(s) 586, 818, 1759, 1778, 2189
AfiI CCNNNNNNNGG 3 cut(s) 487, 1718, 1891
AflIII ACRYGT 2 cut(s) 651, 1398
AgsI TTSAA 7 cut(s) 225, 598, 1557, 1576, 1717, 1867, 2161
AhdI GACNNNNNGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 567, 2230
AjuI GAANNNNNNNTTGG 4 cut(s) 1874, 1906, 2204, 2236
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 4 cut(s) 785, 1407, 1429, 1476
AlwI GGATC 5 cut(s) 1087, 1100, 1191, 1203, 2265
AlwNI CAGNNNCTG 1 cut(s) 1511
AoxI GGCC 7 cut(s) 345, 354, 369, 571, 783, 1369, 2323
ApeKI GCWGC 3 cut(s) 539, 1151, 2241
ApoI RAATTY 5 cut(s) 18, 407, 1460, 1489, 1862
Asp700I GAANNNNTTC 4 cut(s) 489, 653, 749, 1453
AspA2I CCTAGG 1 cut(s) 1256
AspLEI GCGC 1 cut(s) 443
AspS9I GGNCC 8 cut(s) 35, 360, 370, 477, 571, 1252, 1370, 2324
AsuC2I CCSGG 3 cut(s) 358, 887, 1886
AsuHPI GGTGA 4 cut(s) 202, 261, 514, 981
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 4 cut(s) 35, 360, 477, 1252
AvrII CCTAGG 1 cut(s) 1256
BaeI ACNNNNGTAYC 2 cut(s) 2065, 2098
BamHI GGATCC 1 cut(s) 1092
BanII GRGCYC 1 cut(s) 924
BauI CACGAG 2 cut(s) 1022, 1479
Bbv12I GWGCWC 1 cut(s) 910
BbvCI CCTCAGC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 526, 1163, 2253
BccI CCATC 6 cut(s) 683, 821, 967, 1656, 2248, 2295
BceAI ACGGC 1 cut(s) 428
BciT130I CCWGG 2 cut(s) 569, 2232
BciVI GTATCC 1 cut(s) 1911
BclI TGATCA 2 cut(s) 871, 1300
BcnI CCSGG 3 cut(s) 358, 887, 1886
BcoDI GTCTC 4 cut(s) 785, 1407, 1429, 1476
BfaI CTAG 6 cut(s) 1005, 1076, 1257, 1275, 1691, 1755
BfmI CTRYAG 2 cut(s) 820, 1267
BfuI GTATCC 1 cut(s) 1911
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 6 cut(s) 150, 153, 425, 540, 1152, 2242
BlnI CCTAGG 1 cut(s) 1256
BlsI GCNGC 6 cut(s) 151, 154, 426, 541, 1153, 2243
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 5 cut(s) 358, 569, 887, 1886, 2232
Bme18I GGWCC 4 cut(s) 35, 360, 477, 1252
BmeRI GACNNNNNGTC 1 cut(s) 664
BmgT120I GGNCC 8 cut(s) 35, 360, 370, 477, 571, 1252, 1370, 2324
BmiI GGNNCC 6 cut(s) 361, 479, 1094, 1253, 1254, 2001
BmrFI CCNGG 5 cut(s) 358, 569, 887, 1886, 2232
BmrI ACTGGG 2 cut(s) 397, 1258
BmsI GCATC 2 cut(s) 549, 1550
BmuI ACTGGG 2 cut(s) 397, 1258
Bpu10I CCTNAGC 2 cut(s) 374, 1366
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 1339, 1345
BpuMI CCSGG 3 cut(s) 358, 887, 1886
BsaBI GATNNNNATC 2 cut(s) 1182, 1299
BsaI GGTCTC 3 cut(s) 785, 1429, 1476
BsaJI CCNNGG 8 cut(s) 31, 363, 568, 786, 885, 1256, 1547, 2211
BsaWI WCCGGW 1 cut(s) 1786
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 1718, 1891
Bse1I ACTGG 3 cut(s) 392, 1253, 1419
Bse3DI GCAATG 3 cut(s) 466, 612, 1205
Bse8I GATNNNNATC 2 cut(s) 1182, 1299
BseBI CCWGG 2 cut(s) 569, 2232
BseDI CCNNGG 8 cut(s) 31, 363, 568, 786, 885, 1256, 1547, 2211
BseGI GGATG 7 cut(s) 62, 800, 832, 870, 1423, 1667, 2287
BseJI GATNNNNATC 2 cut(s) 1182, 1299
BseLI CCNNNNNNNGG 3 cut(s) 487, 1718, 1891
BseMI GCAATG 3 cut(s) 466, 612, 1205
BseMII CTCAG 5 cut(s) 37, 115, 388, 507, 1380
BseNI ACTGG 3 cut(s) 392, 1253, 1419
BseRI GAGGAG 5 cut(s) 133, 520, 690, 947, 2337
BseXI GCAGC 3 cut(s) 526, 1163, 2253
BseYI CCCAGC 1 cut(s) 1511
BsgI GTGCAG 1 cut(s) 1395
BshFI GGCC 7 cut(s) 347, 356, 371, 573, 785, 1371, 2325
BsiHKAI GWGCWC 1 cut(s) 910
BsiSI CCGG 6 cut(s) 357, 782, 887, 1787, 1886, 2322
BslFI GGGAC 1 cut(s) 1238
BslI CCNNNNNNNGG 3 cut(s) 487, 1718, 1891
BsmAI GTCTC 4 cut(s) 785, 1407, 1429, 1476
BsmFI GGGAC 1 cut(s) 1238
BsnI GGCC 7 cut(s) 347, 356, 371, 573, 785, 1371, 2325
Bso31I GGTCTC 3 cut(s) 785, 1429, 1476
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 910, 924
Bsp143I GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
Bsp19I CCATGG 2 cut(s) 31, 363
BspACI CCGC 7 cut(s) 147, 150, 153, 168, 424, 859, 1096
BspANI GGCC 7 cut(s) 347, 356, 371, 573, 785, 1371, 2325
BspCNI CTCAG 5 cut(s) 36, 114, 387, 508, 1379
BspHI TCATGA 1 cut(s) 2350
BspLI GGNNCC 6 cut(s) 361, 479, 1094, 1253, 1254, 2001
BspPI GGATC 5 cut(s) 1087, 1100, 1191, 1203, 2265
BspT104I TTCGAA 1 cut(s) 16
BspTNI GGTCTC 3 cut(s) 785, 1429, 1476
BsrDI GCAATG 3 cut(s) 466, 612, 1205
BsrI ACTGG 3 cut(s) 392, 1253, 1419
BssECI CCNNGG 8 cut(s) 31, 363, 568, 786, 885, 1256, 1547, 2211
BssMI GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
BssNAI GTATAC 2 cut(s) 232, 1733
BssSI CACGAG 2 cut(s) 1022, 1479
BssT1I CCWWGG 6 cut(s) 31, 363, 786, 1256, 1547, 2211
Bst1107I GTATAC 2 cut(s) 232, 1733
Bst2BI CACGAG 2 cut(s) 1022, 1479
Bst2UI CCWGG 2 cut(s) 569, 2232
Bst4CI ACNGT 9 cut(s) 602, 821, 1102, 1769, 1783, 1871, 1967, 2025, 2248
Bst6I CTCTTC 6 cut(s) 18, 98, 118, 1354, 2046, 2347
BstAPI GCANNNNNTGC 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 5 cut(s) 345, 619, 1212, 1543, 2066
BstDEI CTNAG 7 cut(s) 23, 101, 374, 516, 1066, 1366, 2060
BstDSI CCRYGG 2 cut(s) 31, 363
BstEII GGTNACC 1 cut(s) 208
BstF5I GGATG 7 cut(s) 62, 800, 832, 870, 1423, 1667, 2287
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 8 cut(s) 292, 874, 1095, 1186, 1198, 1303, 1909, 2260
BstMAI GTCTC 4 cut(s) 785, 1407, 1429, 1476
BstMBI GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
BstMWI GCNNNNNNNGC 4 cut(s) 55, 344, 353, 618
BstNI CCWGG 2 cut(s) 569, 2232
BstNSI RCATGY 3 cut(s) 1230, 1402, 1852
BstPI GGTNACC 1 cut(s) 208
BstSCI CCNGG 5 cut(s) 356, 567, 885, 1884, 2230
BstSFI CTRYAG 2 cut(s) 820, 1267
BstV1I GCAGC 3 cut(s) 526, 1163, 2253
BstX2I RGATCY 1 cut(s) 1092
BstXI CCANNNNNNTGG 2 cut(s) 1057, 2231
BstYI RGATCY 1 cut(s) 1092
BstZ17I GTATAC 2 cut(s) 232, 1733
BsuI GTATCC 1 cut(s) 1911
BsuRI GGCC 7 cut(s) 347, 356, 371, 573, 785, 1371, 2325
BtgI CCRYGG 2 cut(s) 31, 363
BtsCI GGATG 7 cut(s) 62, 800, 832, 870, 1423, 1667, 2287
BtsI GCAGTG 1 cut(s) 1050
BtsIMutI CAGTG 6 cut(s) 120, 500, 1050, 1412, 1774, 1779
Cac8I GCNNGC 5 cut(s) 345, 619, 1212, 1543, 2066
CaiI CAGNNNCTG 1 cut(s) 1511
CciI TCATGA 1 cut(s) 2350
CfoI GCGC 1 cut(s) 443
Cfr13I GGNCC 8 cut(s) 35, 360, 370, 477, 571, 1252, 1370, 2324
Csp6I GTAC 5 cut(s) 585, 817, 1758, 1777, 2188
CviQI GTAC 5 cut(s) 585, 817, 1758, 1777, 2188
DdeI CTNAG 7 cut(s) 23, 101, 374, 516, 1066, 1366, 2060
DpnI GATC 8 cut(s) 291, 873, 1094, 1185, 1197, 1302, 1908, 2259
DpnII GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
DraI TTTAAA 2 cut(s) 262, 532
DraIII CACNNNGTG 1 cut(s) 995
DriI GACNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 2 cut(s) 345, 783
Eam1104I CTCTTC 6 cut(s) 18, 98, 118, 1354, 2046, 2347
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 6 cut(s) 18, 98, 118, 1354, 2046, 2347
EciI GGCGGA 1 cut(s) 136
Eco130I CCWWGG 6 cut(s) 31, 363, 786, 1256, 1547, 2211
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 3 cut(s) 785, 1429, 1476
Eco32I GATATC 1 cut(s) 1520
Eco47I GGWCC 4 cut(s) 35, 360, 477, 1252
Eco57I CTGAAG 4 cut(s) 1368, 1574, 1662, 2118
Eco91I GGTNACC 1 cut(s) 208
EcoO109I RGGNCCY 3 cut(s) 477, 571, 1252
EcoO65I GGTNACC 1 cut(s) 208
EcoRI GAATTC 4 cut(s) 18, 1460, 1489, 1862
EcoRII CCWGG 2 cut(s) 567, 2230
EcoRV GATATC 1 cut(s) 1520
EcoT14I CCWWGG 6 cut(s) 31, 363, 786, 1256, 1547, 2211
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 6 cut(s) 31, 363, 786, 1256, 1547, 2211
FalI AAGNNNNNCTT 4 cut(s) 2043, 2075, 2275, 2307
FaqI GGGAC 1 cut(s) 1238
FauNDI CATATG 1 cut(s) 1656
FbaI TGATCA 2 cut(s) 871, 1300
FblI GTMKAC 2 cut(s) 231, 1732
Fnu4HI GCNGC 6 cut(s) 150, 153, 425, 540, 1152, 2242
FokI GGATG 7 cut(s) 49, 787, 839, 877, 1430, 1674, 2274
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 6 cut(s) 150, 153, 425, 540, 1152, 2242
FspBI CTAG 6 cut(s) 1005, 1076, 1257, 1275, 1691, 1755
GlaI GCGC 1 cut(s) 442
GluI GCNGC 6 cut(s) 150, 153, 425, 540, 1152, 2242
GsaI CCCAGC 1 cut(s) 1515
HaeIII GGCC 7 cut(s) 347, 356, 371, 573, 785, 1371, 2325
HapII CCGG 6 cut(s) 357, 782, 887, 1787, 1886, 2322
HhaI GCGC 1 cut(s) 443
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HincII GTYRAC 2 cut(s) 1011, 1105
HindII GTYRAC 2 cut(s) 1011, 1105
HindIII AAGCTT 2 cut(s) 1121, 2034
HinfI GANTC 6 cut(s) 125, 182, 194, 302, 778, 2122
HpaI GTTAAC 1 cut(s) 1105
HpaII CCGG 6 cut(s) 357, 782, 887, 1787, 1886, 2322
HphI GGTGA 4 cut(s) 202, 261, 514, 981
Hpy166II GTNNAC 6 cut(s) 232, 1011, 1105, 1733, 1779, 2155
Hpy8I GTNNAC 6 cut(s) 232, 1011, 1105, 1733, 1779, 2155
Hpy99I CGWCG 4 cut(s) 335, 353, 662, 1615
HpyAV CCTTC 6 cut(s) 127, 973, 1392, 1688, 2301, 2328
HpyCH4III ACNGT 9 cut(s) 602, 821, 1102, 1769, 1783, 1871, 1967, 2025, 2248
HpyCH4IV ACGT 2 cut(s) 651, 1610
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 344, 353, 618
HpyF3I CTNAG 7 cut(s) 23, 101, 374, 516, 1066, 1366, 2060
HpySE526I ACGT 2 cut(s) 651, 1610
HspAI GCGC 1 cut(s) 441
KflI GGGWCCC 1 cut(s) 1252
Ksp22I TGATCA 2 cut(s) 871, 1300
KspAI GTTAAC 1 cut(s) 1105
Kzo9I GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
LmnI GCTCC 4 cut(s) 1687, 1697, 1721, 1999
Lsp1109I GCAGC 3 cut(s) 526, 1163, 2253
LweI GCATC 2 cut(s) 549, 1550
MaeI CTAG 6 cut(s) 1005, 1076, 1257, 1275, 1691, 1755
MaeII ACGT 2 cut(s) 651, 1610
MaeIII GTNAC 6 cut(s) 208, 987, 1000, 1201, 1466, 2119
MalI GATC 8 cut(s) 291, 873, 1094, 1185, 1197, 1302, 1908, 2259
MboI GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
MfeI CAATTG 3 cut(s) 225, 393, 1815
MflI RGATCY 1 cut(s) 1092
MhlI GDGCHC 2 cut(s) 910, 924
MlyI GAGTC 2 cut(s) 119, 2116
MroXI GAANNNNTTC 4 cut(s) 489, 653, 749, 1453
MseI TTAA 9 cut(s) 261, 531, 632, 702, 1104, 1316, 1581, 1854, 2202
MslI CAYNNNNRTG 3 cut(s) 1055, 1293, 2277
MspI CCGG 6 cut(s) 357, 782, 887, 1787, 1886, 2322
MspR9I CCNGG 5 cut(s) 358, 569, 887, 1886, 2232
MunI CAATTG 3 cut(s) 225, 393, 1815
MvaI CCWGG 2 cut(s) 569, 2232
MwoI GCNNNNNNNGC 4 cut(s) 55, 344, 353, 618
NciI CCSGG 3 cut(s) 358, 887, 1886
NcoI CCATGG 2 cut(s) 31, 363
NdeI CATATG 1 cut(s) 1656
NdeII GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
NlaIV GGNNCC 6 cut(s) 361, 479, 1094, 1253, 1254, 2001
NmuCI GTSAC 4 cut(s) 208, 987, 1201, 2119
NspI RCATGY 3 cut(s) 1230, 1402, 1852
NspV TTCGAA 1 cut(s) 16
PagI TCATGA 1 cut(s) 2350
PciI ACATGT 1 cut(s) 1398
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 657
PdmI GAANNNNTTC 4 cut(s) 489, 653, 749, 1453
PfeI GAWTC 4 cut(s) 182, 194, 302, 778
PflMI CCANNNNNTGG 1 cut(s) 487
PfoI TCCNGGA 1 cut(s) 2230
PkrI GCNGC 6 cut(s) 151, 154, 426, 541, 1153, 2243
PleI GAGTC 2 cut(s) 119, 2116
PpsI GAGTC 2 cut(s) 119, 2116
PpuMI RGGWCCY 2 cut(s) 477, 1252
PscI ACATGT 1 cut(s) 1398
Psp5II RGGWCCY 2 cut(s) 477, 1252
Psp6I CCWGG 2 cut(s) 567, 2230
PspEI GGTNACC 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 1511
PspGI CCWGG 2 cut(s) 567, 2230
PspN4I GGNNCC 6 cut(s) 361, 479, 1094, 1253, 1254, 2001
PspPI GGNCC 8 cut(s) 35, 360, 370, 477, 571, 1252, 1370, 2324
PspPPI RGGWCCY 2 cut(s) 477, 1252
PsrI GAACNNNNNNTAC 2 cut(s) 1816, 1848
PstNI CAGNNNCTG 1 cut(s) 1511
PsuI RGATCY 1 cut(s) 1092
RsaI GTAC 5 cut(s) 586, 818, 1759, 1778, 2189
RsaNI GTAC 5 cut(s) 585, 817, 1758, 1777, 2188
RseI CAYNNNNRTG 3 cut(s) 1055, 1293, 2277
SaqAI TTAA 9 cut(s) 261, 531, 632, 702, 1104, 1316, 1581, 1854, 2202
SatI GCNGC 6 cut(s) 150, 153, 425, 540, 1152, 2242
Sau3AI GATC 8 cut(s) 289, 871, 1092, 1183, 1195, 1300, 1906, 2257
Sau96I GGNCC 8 cut(s) 35, 360, 370, 477, 571, 1252, 1370, 2324
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 2 cut(s) 119, 2116
ScrFI CCNGG 5 cut(s) 358, 569, 887, 1886, 2232
SduI GDGCHC 2 cut(s) 910, 924
SfaNI GCATC 2 cut(s) 549, 1550
SfcI CTRYAG 2 cut(s) 820, 1267
SfiI GGCCNNNNNGGCC 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 4 cut(s) 35, 360, 477, 1252
SmiMI CAYNNNNRTG 3 cut(s) 1055, 1293, 2277
SmlI CTYRAG 2 cut(s) 1354, 1360
SmoI CTYRAG 2 cut(s) 1354, 1360
SsiI CCGC 7 cut(s) 147, 150, 153, 168, 424, 859, 1096
SspI AATATT 2 cut(s) 835, 1579
SspMI CTAG 6 cut(s) 1005, 1076, 1257, 1275, 1691, 1755
StyD4I CCNGG 5 cut(s) 356, 567, 885, 1884, 2230
StyI CCWWGG 6 cut(s) 31, 363, 786, 1256, 1547, 2211
TaaI ACNGT 9 cut(s) 602, 821, 1102, 1769, 1783, 1871, 1967, 2025, 2248
TaiI ACGT 2 cut(s) 654, 1613
TaqI TCGA 6 cut(s) 16, 412, 555, 657, 753, 2334
TaqII GACCGA 2 cut(s) 1395, 1603
TatI WGTACW 3 cut(s) 584, 1757, 1776
TauI GCSGC 3 cut(s) 152, 155, 427
TfiI GAWTC 4 cut(s) 182, 194, 302, 778
Tru1I TTAA 9 cut(s) 261, 531, 632, 702, 1104, 1316, 1581, 1854, 2202
Tru9I TTAA 9 cut(s) 261, 531, 632, 702, 1104, 1316, 1581, 1854, 2202
TscAI CASTG 6 cut(s) 127, 500, 1057, 1419, 1774, 1786
TseFI GTSAC 4 cut(s) 208, 987, 1201, 2119
TseI GCWGC 3 cut(s) 539, 1151, 2241
Tsp45I GTSAC 4 cut(s) 208, 987, 1201, 2119
TspDTI ATGAA 6 cut(s) 147, 498, 746, 1462, 1953, 2046
TspGWI ACGGA 2 cut(s) 319, 1454
TspRI CASTG 6 cut(s) 127, 500, 1057, 1419, 1774, 1786
Van91I CCANNNNNTGG 1 cut(s) 487
VpaK11BI GGWCC 4 cut(s) 35, 360, 477, 1252
XapI RAATTY 5 cut(s) 18, 407, 1460, 1489, 1862
XceI RCATGY 3 cut(s) 1230, 1402, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XmaJI CCTAGG 1 cut(s) 1256
XmiI GTMKAC 2 cut(s) 231, 1732
XmnI GAANNNNTTC 4 cut(s) 489, 653, 749, 1453
XspI CTAG 6 cut(s) 1005, 1076, 1257, 1275, 1691, 1755
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.