Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007335

Physical Location & Seq

Genomic Coordinates Forward (+)

80014126 .. 80016587

Gene Structure 2462 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00058120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGTTGTTTCTCTTCGAATTCTCAGAAACCATGGATTTCAGCACAAGCTATTATCGCCTACATCCACCCACATTTCCCGACTCTACACCCTCTTCTCTCTCAGAACCCTCTCTTCCTTCACTGACTCCGATGAACCATACTCCTCCGCCGCCGTCACCACCTCCGATTCCCAATCAGAATCACAGTCTCTGGTCACCACAATCTGTTCAATGGTATACAAATCCTACTCCCCACAAACCCATTTCAAATCTTCACCTCCAAACCTCAATCTTGATCTAAATCCCGATTCTTTGACCCACGAACACGCCATTTCCGTCGTGGCTTCACTTTCCGACGAGGCCGGGTCCATGGTGGCCCTCAGCTTCTTCTACTGGGCAATTGGGTTTGCGAAATTTCGATACTTTATGCGGCTTTATATCTTCTGCGCCATGTCGATATTCGGCAATGGCAATTTGGAGAGGACCCATGAAGTGGTTCAGTGTATGGTGAGGAGTTTCGCTGAGATTGGGAGGTTTAAAGAGGCTGCCGATATGGTTTTCGAGATGCAAAACCAGGGCCTTGTGTTGAGTACTCGGACTTTGAACTGTGTCATCGGCATTGCTTGCGAAATGGGTTTGATGGAATATGCAGAGAACGTGTTCGACGAAATGTCTGTGAGAGGAGTGTGTCCTGATTCTTTGAGCTTTAAGTGTATGGTTGTTGGGTATTGTCGAAACGGTGCGGTTTTGGAGGTGGATAGGTGGTTGAGTAAGATGATTGAGAGGGGCTTTGTTTTGGATAATGCTAGTTTTACTTTGATTATTAGTGTGTTTTGTGAGAAGGGTTTTGTGAGTAGAGCGTCTTGGTGTTTCGATAAGATGAGTAAGATGGGGGTGAAGCCGAATTTGGTTAACTTTACGTCTTTGATACATGGGTTGTGCAAGAGGGGGAGTGTTAAGCAGGCGTTTGAGATGTTGGAGGAGATGGTTAGGAGAGGTTGGAAGCCGAATGTGTATACACATACTGCGTTGATTGATGGGTTGTGCAAGAAAGGTTGGACTGAGAGGGCGTTTAGGCTGTTTCTGAAGCTTGTCCGGAGTGATAGTTATAAGCCAAATGTGCATACTTATACTGCGATGATCAGTGGATATTGCAAGGAGGATAAAATGAGCCGTGCGGAGATGTTGTTGAGCAGAATGAAGGAACAAGAATTAGTTCCTAACACCAATACGTATACTACTCTTGTTTATGGGCATTGCAAAGCTGGGAATTTTGAAAGAGCATATGAATTGATGGATGTAATGAGTGAAGAAGGATTTGCTCCGAATATATGTACATACAATGCAGTTATGGACAGTCTCTGTAAAAAGGGGAGGGTCCAAGAAGCTTATAAACTGATTAAGAAGGGCCTTCGCCGAGGATTGCAGGCTGATAGAGTCACATATACCATTTTCATATCCGAGCACTGCAAACGGGGTGATGTTAACAGTGCCCTAGCTTTTTTCAATAAGATGGTCAAGGCTGGATTGGAGCCTGATATACATTCATATACCACTTTGATTGCTGCCTTCTGCAGGCAAAAGAAAATGAAAGAAAGCGAGAAACTTTTCGAGGTAGCTGAAAGGCTTGGCTTGATTCCGGCCAAGGAGACCTATACATCCATGATATGTGGGTACTGTAGGGATGGAAATATTGCTTTGGCTGTAAAGTTCTTCCTCAGGATGAGTGATCATGGTTGTTCCCCAGATAGGTTTACTTATGGTGCTCTAATAAGTGGGCTCTGCAAGGAGGAAAAACTTGACGAGGCTCGTAAGTTATATGATACCATGATGGACAAGGGGCTGTCACCCTGTGAAGTTACTAGGTTGACATTAGCTCACGAGTATTGCCAAAAAGATGACTATGCCACTGCTATGGTCATCTTAGATAGGCTAGACAAGAAGTTCTGGATCCGCACGGTTAACACATTGGTCAGGAAGCTTTGTTGTGAGAAGAAAGTGGGAATAGCAGCATTGTTCTTTCACAAATTAGTTGATAAGGATCAGAATGTGGATCGTGTGACATTGCAAGCATTTATGACTGCATGTTATGAGAGCAACAAGTATGCTCTTCTTTCGGATTTGACTGAGAGAATCTCTAAAGGAATTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

710

Amino Acids

80.59

Weight (kDa)

8.67

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	191 - 238	2.5e-08	PPR repeat family
PPR_2	PF13041	262 - 308	3.7e-09	PPR repeat family
PPR_1	PF12854	290 - 323	2e-12	PPR repeat
PPR_2	PF13041	294 - 343	2.2e-18	PPR repeat family
PPR	PF01535	299 - 326	7.5e-07	PPR repeat
PPR_3	PF13812	318 - 378	1.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	326 - 354	1.9e-09	PPR repeat
PPR_2	PF13041	335 - 378	1.4e-09	PPR repeat family
PPR_long	PF17177	348 - 473	1.1e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	356 - 459	6.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	363 - 394	2.2e-09	PPR repeat
PPR_2	PF13041	365 - 414	2.3e-15	PPR repeat family
PPR	PF01535	369 - 396	1.9e-07	PPR repeat
PPR_3	PF13812	389 - 445	2.9e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	398 - 428	6.5e-10	PPR repeat
PPR_2	PF13041	404 - 449	4.9e-12	PPR repeat family
PPR	PF01535	404 - 433	6.1e-06	PPR repeat
PPR_1	PF12854	432 - 461	5.2e-09	PPR repeat
PPR_2	PF13041	435 - 484	7.4e-14	PPR repeat family
PPR	PF01535	439 - 468	9.4e-06	PPR repeat
PPR_1	PF12854	466 - 499	3.3e-09	PPR repeat
PPR_2	PF13041	471 - 519	5.1e-16	PPR repeat family
PPR_long	PF17177	478 - 638	3.4e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	502 - 531	3.9e-07	PPR repeat
PPR_2	PF13041	505 - 551	1.2e-08	PPR repeat family
PPR_3	PF13812	529 - 585	6.9e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	539 - 568	1.1e-06	PPR repeat
PPR	PF01535	544 - 573	5.7e-07	PPR repeat
PPR_2	PF13041	544 - 589	6.6e-15	PPR repeat family
PPR_1	PF12854	572 - 603	1.4e-11	PPR repeat
PPR	PF01535	578 - 608	5.5e-07	PPR repeat
PPR_2	PF13041	578 - 623	1.9e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19890
fragaria_vesca	FvH4_3g12270 FvH4_3g12270 FvH4_3g12270
malus_domestica	MD05G1251800.v1.1
prunus_persica	Prupe.4G111800_v2.0.a1
pyrus_communis	pycom05g22980 pycom10g19560
rosa_chinensis	RchiOBHm_Chr5g0020081 RchiOBHm_Chr7g0211421
rosa_laevigata	RLG00000032497
rosa_multiflora	Rmu_sc0021557.1_g000003
rosa_roxburghii	Rroxscaffold_1G00058120
rosa_rugosa	Rorug05G0056700
rosa_samantha	Rh5AG146800 Rh5BG146300 Rh5CG157900 Rh5DG145800 Rh6CG070300 Rh6DG067500 Rh7AG292200 Rh7CG312200 Rh7DG257000 Rh7DG263700
rosa_wichuraiana	Rw5G012970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1089, 1371
AccB7I	CCANNNNNTGG	1 cut(s)	472
AccI	GTMKAC	3 cut(s)	216, 995, 1214
AccIII	TCCGGA	1 cut(s)	1074
AciI	CCGC	6 cut(s)	147, 150, 409, 722, 1157, 1933
AclWI	GGATC	4 cut(s)	1924, 1937, 2028, 2040
AcoI	YGGCCR	1 cut(s)	1620
AcsI	RAATTY	4 cut(s)	18, 392, 883, 1249
AcuI	CTGAAG	1 cut(s)	1085
AdeI	CACNNNGTG	1 cut(s)	1832
AfaI	GTAC	3 cut(s)	571, 1315, 1655
AfiI	CCNNNNNNNGG	3 cut(s)	472, 1554, 1728
AflIII	ACRYGT	1 cut(s)	636
AgsI	TTSAA	5 cut(s)	210, 247, 583, 1256, 1486
AhdI	GACNNNNNGTC	1 cut(s)	649
AjnI	CCWGG	1 cut(s)	552
AjuI	GAANNNNNNNTTGG	2 cut(s)	869, 901
Alw21I	GWGCWC	2 cut(s)	1446, 1747
Alw26I	GTCTC	3 cut(s)	192, 1343, 1622
AlwI	GGATC	4 cut(s)	1924, 1937, 2028, 2040
AlwNI	CAGNNNCTG	2 cut(s)	190, 1341
Aor13HI	TCCGGA	1 cut(s)	1074
AoxI	GGCC	5 cut(s)	339, 354, 556, 1387, 1620
ApeKI	GCWGC	3 cut(s)	524, 1544, 1988
ApoI	RAATTY	4 cut(s)	18, 392, 883, 1249
Asp700I	GAANNNNTTC	4 cut(s)	474, 638, 1194, 1586
AspLEI	GCGC	1 cut(s)	428
AspS9I	GGNCC	6 cut(s)	345, 355, 462, 556, 1357, 1387
AsuC2I	CCSGG	1 cut(s)	343
AsuHPI	GGTGA	7 cut(s)	148, 187, 246, 499, 886, 1469, 1818
AsuII	TTCGAA	1 cut(s)	16
AvaII	GGWCC	3 cut(s)	345, 462, 1357
AxyI	CCTNAGG	1 cut(s)	1697
BaeGI	GKGCMC	1 cut(s)	1474
BaeI	ACNNNNGTAYC	2 cut(s)	899, 932
BamHI	GGATCC	1 cut(s)	1929
BanII	GRGCYC	1 cut(s)	1761
BauI	CACGAG	1 cut(s)	1859
Bbv12I	GWGCWC	2 cut(s)	1446, 1747
BbvCI	CCTCAGC	1 cut(s)	359
BbvI	GCAGC	3 cut(s)	511, 1531, 2000
BccI	CCATC	8 cut(s)	613, 862, 958, 1010, 1267, 1486, 1658, 1804
BceAI	ACGGC	2 cut(s)	137, 1137
BciT130I	CCWGG	1 cut(s)	554
BclI	TGATCA	2 cut(s)	1119, 1708
BcnI	CCSGG	1 cut(s)	343
BcoDI	GTCTC	3 cut(s)	192, 1343, 1622
BfaI	CTAG	5 cut(s)	786, 1475, 1842, 1913, 2131
BfmI	CTRYAG	2 cut(s)	1552, 1657
BisI	GCNGC	5 cut(s)	150, 410, 525, 1545, 1989
BlsI	GCNGC	5 cut(s)	151, 411, 526, 1546, 1990
BmcAI	AGTACT	1 cut(s)	571
Bme1390I	CCNGG	2 cut(s)	343, 554
Bme18I	GGWCC	3 cut(s)	345, 462, 1357
BmeRI	GACNNNNNGTC	1 cut(s)	649
BmgT120I	GGNCC	6 cut(s)	345, 355, 462, 556, 1357, 1387
BmiI	GGNNCC	5 cut(s)	346, 464, 1358, 1512, 1931
BmrFI	CCNGG	2 cut(s)	343, 554
BmrI	ACTGGG	1 cut(s)	382
BmsI	GCATC	1 cut(s)	534
BmuI	ACTGGG	1 cut(s)	382
BplI	GAGNNNNNCTC	2 cut(s)	2099, 2131
Bpu10I	CCTNAGC	1 cut(s)	359
Bpu14I	TTCGAA	1 cut(s)	16
BpuMI	CCSGG	1 cut(s)	343
BsaAI	YACGTR	1 cut(s)	1212
BsaBI	GATNNNNATC	2 cut(s)	279, 2019
BsaI	GGTCTC	1 cut(s)	1622
BsaJI	CCNNGG	5 cut(s)	31, 348, 553, 1396, 1623
BsaWI	WCCGGW	1 cut(s)	1074
BsaXI	ACNNNNNCTCC	4 cut(s)	1069, 1099, 1151, 1181
Bsc4I	CCNNNNNNNGG	3 cut(s)	472, 1554, 1728
Bse1I	ACTGG	1 cut(s)	377
Bse21I	CCTNAGG	1 cut(s)	1697
Bse3DI	GCAATG	4 cut(s)	451, 597, 1234, 2042
Bse8I	GATNNNNATC	2 cut(s)	279, 2019
BseAI	TCCGGA	1 cut(s)	1074
BseBI	CCWGG	1 cut(s)	554
BseDI	CCNNGG	5 cut(s)	31, 348, 553, 1396, 1623
BseGI	GGATG	5 cut(s)	62, 1282, 1637, 1669, 1707
BseJI	GATNNNNATC	2 cut(s)	279, 2019
BseLI	CCNNNNNNNGG	3 cut(s)	472, 1554, 1728
BseMI	GCAATG	4 cut(s)	451, 597, 1234, 2042
BseMII	CTCAG	7 cut(s)	37, 115, 373, 492, 1032, 1711, 2097
BseNI	ACTGG	1 cut(s)	377
BseRI	GAGGAG	4 cut(s)	133, 505, 675, 974
BseSI	GKGCMC	1 cut(s)	1474
BseXI	GCAGC	3 cut(s)	511, 1531, 2000
BseYI	CCCAGC	1 cut(s)	1244
BshFI	GGCC	5 cut(s)	341, 356, 558, 1389, 1622
BsiHKAI	GWGCWC	2 cut(s)	1446, 1747
BsiSI	CCGG	3 cut(s)	342, 1075, 1619
BslI	CCNNNNNNNGG	3 cut(s)	472, 1554, 1728
BsmAI	GTCTC	3 cut(s)	192, 1343, 1622
BsnI	GGCC	5 cut(s)	341, 356, 558, 1389, 1622
Bso31I	GGTCTC	1 cut(s)	1622
Bsp119I	TTCGAA	1 cut(s)	16
Bsp1286I	GDGCHC	4 cut(s)	1446, 1474, 1747, 1761
Bsp13I	TCCGGA	1 cut(s)	1074
Bsp1407I	TGTACA	1 cut(s)	1313
Bsp143I	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
Bsp19I	CCATGG	2 cut(s)	31, 348
BspACI	CCGC	6 cut(s)	147, 150, 409, 722, 1157, 1933
BspANI	GGCC	5 cut(s)	341, 356, 558, 1389, 1622
BspCNI	CTCAG	7 cut(s)	36, 114, 372, 493, 1033, 1710, 2098
BspEI	TCCGGA	1 cut(s)	1074
BspLI	GGNNCC	5 cut(s)	346, 464, 1358, 1512, 1931
BspMAI	CTGCAG	1 cut(s)	1556
BspPI	GGATC	4 cut(s)	1924, 1937, 2028, 2040
BspQI	GCTCTTC	1 cut(s)	2094
BspT104I	TTCGAA	1 cut(s)	16
BspTNI	GGTCTC	1 cut(s)	1622
BsrDI	GCAATG	4 cut(s)	451, 597, 1234, 2042
BsrGI	TGTACA	1 cut(s)	1313
BsrI	ACTGG	1 cut(s)	377
BssECI	CCNNGG	5 cut(s)	31, 348, 553, 1396, 1623
BssMI	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
BssNAI	GTATAC	3 cut(s)	217, 996, 1215
BssSI	CACGAG	1 cut(s)	1859
BssT1I	CCWWGG	3 cut(s)	31, 348, 1623
Bst1107I	GTATAC	3 cut(s)	217, 996, 1215
Bst2BI	CACGAG	1 cut(s)	1859
Bst2UI	CCWGG	1 cut(s)	554
Bst4CI	ACNGT	7 cut(s)	186, 587, 719, 1337, 1469, 1658, 1939
Bst6I	CTCTTC	4 cut(s)	18, 98, 118, 2094
BstAPI	GCANNNNNTGC	1 cut(s)	603
BstAUI	TGTACA	1 cut(s)	1313
BstBAI	YACGTR	1 cut(s)	1212
BstBI	TTCGAA	1 cut(s)	16
BstC8I	GCNNGC	5 cut(s)	604, 942, 1407, 1556, 2049
BstDEI	CTNAG	8 cut(s)	23, 101, 359, 501, 1041, 1697, 1903, 2106
BstDSI	CCRYGG	2 cut(s)	31, 348
BstEII	GGTNACC	1 cut(s)	193
BstENI	CCTNNNNNAGG	1 cut(s)	1552
BstF5I	GGATG	5 cut(s)	62, 1282, 1637, 1669, 1707
BstHHI	GCGC	1 cut(s)	428
BstKTI	GATC	6 cut(s)	277, 1122, 1711, 1932, 2023, 2035
BstMAI	GTCTC	3 cut(s)	192, 1343, 1622
BstMBI	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
BstMWI	GCNNNNNNNGC	3 cut(s)	55, 603, 1099
BstNI	CCWGG	1 cut(s)	554
BstNSI	RCATGY	1 cut(s)	2067
BstPI	GGTNACC	1 cut(s)	193
BstSCI	CCNGG	2 cut(s)	341, 552
BstSFI	CTRYAG	2 cut(s)	1552, 1657
BstSLI	GKGCMC	1 cut(s)	1474
BstSNI	TACGTA	1 cut(s)	1212
BstV1I	GCAGC	3 cut(s)	511, 1531, 2000
BstX2I	RGATCY	1 cut(s)	1929
BstXI	CCANNNNNNTGG	1 cut(s)	1894
BstYI	RGATCY	1 cut(s)	1929
BstZ17I	GTATAC	3 cut(s)	217, 996, 1215
Bsu36I	CCTNAGG	1 cut(s)	1697
BsuRI	GGCC	5 cut(s)	341, 356, 558, 1389, 1622
BtgI	CCRYGG	2 cut(s)	31, 348
BtgZI	GCGATG	1 cut(s)	1130
BtsCI	GGATG	5 cut(s)	62, 1282, 1637, 1669, 1707
BtsI	GCAGTG	2 cut(s)	1444, 1887
BtsIMutI	CAGTG	6 cut(s)	120, 485, 1129, 1444, 1474, 1887
Cac8I	GCNNGC	5 cut(s)	604, 942, 1407, 1556, 2049
CaiI	CAGNNNCTG	2 cut(s)	190, 1341
CfoI	GCGC	1 cut(s)	428
Cfr13I	GGNCC	6 cut(s)	345, 355, 462, 556, 1357, 1387
CseI	GACGC	1 cut(s)	828
Csp6I	GTAC	3 cut(s)	570, 1314, 1654
CspCI	CAANNNNNGTGG	2 cut(s)	1522, 1557
CviAII	CATG	9 cut(s)	32, 349, 430, 467, 911, 1642, 1712, 1807, 2064
CviQI	GTAC	3 cut(s)	570, 1314, 1654
DdeI	CTNAG	8 cut(s)	23, 101, 359, 501, 1041, 1697, 1903, 2106
DpnI	GATC	6 cut(s)	276, 1121, 1710, 1931, 2022, 2034
DpnII	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
DraI	TTTAAA	1 cut(s)	517
DraIII	CACNNNGTG	1 cut(s)	1832
DriI	GACNNNNNGTC	1 cut(s)	649
EaeI	YGGCCR	1 cut(s)	1620
Eam1104I	CTCTTC	4 cut(s)	18, 98, 118, 2094
Eam1105I	GACNNNNNGTC	1 cut(s)	649
EarI	CTCTTC	4 cut(s)	18, 98, 118, 2094
EciI	GGCGGA	1 cut(s)	136
Eco105I	TACGTA	1 cut(s)	1212
Eco130I	CCWWGG	3 cut(s)	31, 348, 1623
Eco24I	GRGCYC	1 cut(s)	1761
Eco31I	GGTCTC	1 cut(s)	1622
Eco47I	GGWCC	3 cut(s)	345, 462, 1357
Eco57I	CTGAAG	1 cut(s)	1085
Eco81I	CCTNAGG	1 cut(s)	1697
Eco91I	GGTNACC	1 cut(s)	193
EcoNI	CCTNNNNNAGG	1 cut(s)	1552
EcoO109I	RGGNCCY	3 cut(s)	462, 556, 1387
EcoO65I	GGTNACC	1 cut(s)	193
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	1 cut(s)	552
EcoT14I	CCWWGG	3 cut(s)	31, 348, 1623
EcoT38I	GRGCYC	1 cut(s)	1761
ErhI	CCWWGG	3 cut(s)	31, 348, 1623
FaeI	CATG	9 cut(s)	35, 352, 433, 470, 914, 1645, 1715, 1810, 2067
FatI	CATG	9 cut(s)	31, 348, 429, 466, 910, 1641, 1711, 1806, 2063
FauNDI	CATATG	1 cut(s)	1264
FbaI	TGATCA	2 cut(s)	1119, 1708
FblI	GTMKAC	3 cut(s)	216, 995, 1214
Fnu4HI	GCNGC	5 cut(s)	150, 410, 525, 1545, 1989
FokI	GGATG	5 cut(s)	49, 1289, 1624, 1676, 1714
FriOI	GRGCYC	1 cut(s)	1761
Fsp4HI	GCNGC	5 cut(s)	150, 410, 525, 1545, 1989
FspBI	CTAG	5 cut(s)	786, 1475, 1842, 1913, 2131
GlaI	GCGC	1 cut(s)	427
GluI	GCNGC	5 cut(s)	150, 410, 525, 1545, 1989
GsaI	CCCAGC	1 cut(s)	1248
HaeIII	GGCC	5 cut(s)	341, 356, 558, 1389, 1622
HapII	CCGG	3 cut(s)	342, 1075, 1619
HgaI	GACGC	1 cut(s)	828
HhaI	GCGC	1 cut(s)	428
Hin1II	CATG	9 cut(s)	35, 352, 433, 470, 914, 1645, 1715, 1810, 2067
Hin6I	GCGC	1 cut(s)	426
HinP1I	GCGC	1 cut(s)	426
HincII	GTYRAC	4 cut(s)	892, 1465, 1848, 1942
HindII	GTYRAC	4 cut(s)	892, 1465, 1848, 1942
HindIII	AAGCTT	3 cut(s)	1067, 1365, 1958
HinfI	GANTC	9 cut(s)	81, 125, 167, 179, 287, 674, 1416, 1615, 2112
HpaI	GTTAAC	3 cut(s)	892, 1465, 1942
HpaII	CCGG	3 cut(s)	342, 1075, 1619
HphI	GGTGA	7 cut(s)	148, 187, 246, 499, 886, 1469, 1818
Hpy166II	GTNNAC	8 cut(s)	217, 892, 996, 1215, 1465, 1734, 1848, 1942
Hpy8I	GTNNAC	8 cut(s)	217, 892, 996, 1215, 1465, 1734, 1848, 1942
Hpy99I	CGWCG	3 cut(s)	320, 338, 647
HpyAV	CCTTC	7 cut(s)	127, 814, 1174, 1286, 1378, 1400, 1558
HpyCH4III	ACNGT	7 cut(s)	186, 587, 719, 1337, 1469, 1658, 1939
HpyCH4IV	ACGT	3 cut(s)	636, 899, 1211
HpyF10VI	GCNNNNNNNGC	3 cut(s)	55, 603, 1099
HpyF3I	CTNAG	8 cut(s)	23, 101, 359, 501, 1041, 1697, 1903, 2106
HpySE526I	ACGT	3 cut(s)	636, 899, 1211
Hsp92II	CATG	9 cut(s)	35, 352, 433, 470, 914, 1645, 1715, 1810, 2067
HspAI	GCGC	1 cut(s)	426
Kpn2I	TCCGGA	1 cut(s)	1074
Ksp22I	TGATCA	2 cut(s)	1119, 1708
KspAI	GTTAAC	3 cut(s)	892, 1465, 1942
Kzo9I	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
LguI	GCTCTTC	1 cut(s)	2094
LmnI	GCTCC	2 cut(s)	1306, 1510
Lsp1109I	GCAGC	3 cut(s)	511, 1531, 2000
LweI	GCATC	1 cut(s)	534
MaeI	CTAG	5 cut(s)	786, 1475, 1842, 1913, 2131
MaeII	ACGT	3 cut(s)	636, 899, 1211
MaeIII	GTNAC	6 cut(s)	154, 193, 1417, 1824, 1837, 2038
MalI	GATC	6 cut(s)	276, 1121, 1710, 1931, 2022, 2034
MboI	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
MfeI	CAATTG	1 cut(s)	378
MflI	RGATCY	1 cut(s)	1929
MhlI	GDGCHC	4 cut(s)	1446, 1474, 1747, 1761
MlyI	GAGTC	3 cut(s)	75, 119, 1425
MmeI	TCCRAC	4 cut(s)	357, 936, 959, 1016
MroI	TCCGGA	1 cut(s)	1074
MroXI	GAANNNNTTC	4 cut(s)	474, 638, 1194, 1586
MseI	TTAA	7 cut(s)	516, 687, 891, 936, 1380, 1464, 1941
MslI	CAYNNNNRTG	1 cut(s)	1892
MspI	CCGG	3 cut(s)	342, 1075, 1619
MspR9I	CCNGG	2 cut(s)	343, 554
MunI	CAATTG	1 cut(s)	378
MvaI	CCWGG	1 cut(s)	554
MwoI	GCNNNNNNNGC	3 cut(s)	55, 603, 1099
NciI	CCSGG	1 cut(s)	343
NcoI	CCATGG	2 cut(s)	31, 348
NdeI	CATATG	1 cut(s)	1264
NdeII	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
NlaIII	CATG	9 cut(s)	35, 352, 433, 470, 914, 1645, 1715, 1810, 2067
NlaIV	GGNNCC	5 cut(s)	346, 464, 1358, 1512, 1931
NmeAIII	GCCGAG	1 cut(s)	1421
NmuCI	GTSAC	5 cut(s)	154, 193, 1417, 1824, 2038
NspI	RCATGY	1 cut(s)	2067
NspV	TTCGAA	1 cut(s)	16
PciSI	GCTCTTC	1 cut(s)	2094
PcsI	WCGNNNNNNNCGW	2 cut(s)	312, 642
PdmI	GAANNNNTTC	4 cut(s)	474, 638, 1194, 1586
PfeI	GAWTC	6 cut(s)	167, 179, 287, 674, 1615, 2112
PflMI	CCANNNNNTGG	1 cut(s)	472
PkrI	GCNGC	5 cut(s)	151, 411, 526, 1546, 1990
PleI	GAGTC	3 cut(s)	75, 119, 1424
PpsI	GAGTC	3 cut(s)	75, 119, 1424
Ppu21I	YACGTR	1 cut(s)	1212
PpuMI	RGGWCCY	1 cut(s)	462
PsiI	TTATAA	2 cut(s)	1089, 1371
Psp5II	RGGWCCY	1 cut(s)	462
Psp6I	CCWGG	1 cut(s)	552
PspEI	GGTNACC	1 cut(s)	193
PspFI	CCCAGC	1 cut(s)	1244
PspGI	CCWGG	1 cut(s)	552
PspN4I	GGNNCC	5 cut(s)	346, 464, 1358, 1512, 1931
PspPI	GGNCC	6 cut(s)	345, 355, 462, 556, 1357, 1387
PspPPI	RGGWCCY	1 cut(s)	462
PstI	CTGCAG	1 cut(s)	1556
PstNI	CAGNNNCTG	2 cut(s)	190, 1341
PsuI	RGATCY	1 cut(s)	1929
RsaI	GTAC	3 cut(s)	571, 1315, 1655
RsaNI	GTAC	3 cut(s)	570, 1314, 1654
RseI	CAYNNNNRTG	1 cut(s)	1892
SapI	GCTCTTC	1 cut(s)	2094
SaqAI	TTAA	7 cut(s)	516, 687, 891, 936, 1380, 1464, 1941
SatI	GCNGC	5 cut(s)	150, 410, 525, 1545, 1989
Sau3AI	GATC	6 cut(s)	274, 1119, 1708, 1929, 2020, 2032
Sau96I	GGNCC	6 cut(s)	345, 355, 462, 556, 1357, 1387
ScaI	AGTACT	1 cut(s)	571
SchI	GAGTC	3 cut(s)	75, 119, 1425
ScrFI	CCNGG	2 cut(s)	343, 554
SduI	GDGCHC	4 cut(s)	1446, 1474, 1747, 1761
SfaNI	GCATC	1 cut(s)	534
SfcI	CTRYAG	2 cut(s)	1552, 1657
SfuI	TTCGAA	1 cut(s)	16
SinI	GGWCC	3 cut(s)	345, 462, 1357
SmiMI	CAYNNNNRTG	1 cut(s)	1892
SnaBI	TACGTA	1 cut(s)	1212
SsiI	CCGC	6 cut(s)	147, 150, 409, 722, 1157, 1933
SspI	AATATT	1 cut(s)	1672
SspMI	CTAG	5 cut(s)	786, 1475, 1842, 1913, 2131
StyD4I	CCNGG	2 cut(s)	341, 552
StyI	CCWWGG	3 cut(s)	31, 348, 1623
TaaI	ACNGT	7 cut(s)	186, 587, 719, 1337, 1469, 1658, 1939
TaiI	ACGT	3 cut(s)	639, 902, 1214
TaqI	TCGA	8 cut(s)	16, 397, 434, 540, 642, 712, 852, 1590
TatI	WGTACW	2 cut(s)	569, 1313
TauI	GCSGC	2 cut(s)	152, 412
TfiI	GAWTC	6 cut(s)	167, 179, 287, 674, 1615, 2112
Tru1I	TTAA	7 cut(s)	516, 687, 891, 936, 1380, 1464, 1941
Tru9I	TTAA	7 cut(s)	516, 687, 891, 936, 1380, 1464, 1941
TscAI	CASTG	6 cut(s)	127, 485, 1129, 1451, 1474, 1894
TseFI	GTSAC	5 cut(s)	154, 193, 1417, 1824, 2038
TseI	GCWGC	3 cut(s)	524, 1544, 1988
Tsp45I	GTSAC	5 cut(s)	154, 193, 1417, 1824, 2038
TspDTI	ATGAA	7 cut(s)	147, 483, 1193, 1281, 1423, 1515, 1583
TspGWI	ACGGA	1 cut(s)	304
TspRI	CASTG	6 cut(s)	127, 485, 1129, 1451, 1474, 1894
Van91I	CCANNNNNTGG	1 cut(s)	472
VpaK11BI	GGWCC	3 cut(s)	345, 462, 1357
XagI	CCTNNNNNAGG	1 cut(s)	1552
XapI	RAATTY	4 cut(s)	18, 392, 883, 1249
XceI	RCATGY	1 cut(s)	2067
XcmI	CCANNNNNNNNNTGG	1 cut(s)	316
XmiI	GTMKAC	3 cut(s)	216, 995, 1214
XmnI	GAANNNNTTC	4 cut(s)	474, 638, 1194, 1586
XspI	CTAG	5 cut(s)	786, 1475, 1842, 1913, 2131
ZrmI	AGTACT	1 cut(s)	571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.