Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0007335

Physical Location & Seq

Genomic Coordinates Reverse (-)

15861328 .. 15863463

Gene Structure 2136 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032497

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGTTGTTTCTCTTCGAATTCTCAGAAACCATGGACTTCAACACAAGCTGTTATCGCCTACATCGACCCACATTTCTCAACTCAAGAGAACCCTCTCTTCCTTCACTGACTCCGATGAACCATACTCCTCCGCCGCCGCCACCACAACCACCACCACCACCACCTCCGATTCCCAATCAGAATCACACTCTCTGGTCACCACAATCTGTTCAATGGTATACAAATCCTATTCCCCACAAACCCATTTCAAGTCTTCACCTCCAAAGCTCAACCTTGATTTAGATTCTGACTCCTTGACCCACGAACACGCCATTTCCGTCATGGCTTCGCTGGCCGACGAGGCCGGGTCCATGGTGGCCCTCAGCTTTTTCTACTGGGCAATTGAGTTTGCGAAATTTCGATACTTTATGCGGCTTTACATCTTCTGCGCCATGTCGATTTTCGGCAATGGCAATTTGGCGAGGACCCATGAAGTGGTTCAGTGTATGGTGAGGAGTTTCGCTGAGATTGGGAGGTTTAAAGAGGCTGCCGATATGGTTTTCGAGATGCAAAACCAGGGCCTTGTGTTGAGTACTCGCAGTTTGAACTGTGTCATCGGCATTGCTTGCGAAATGGGTTTGATGGATTATGCAGAGAACGTGTTCGACGAAATGTCTGTGAGAGGAGTGTGTCCTGATGGTTTGAGCTTTAAGTGTATGGTTGTTGGGTATTGTCGAAACGGTGCGGTTTTGGAGGTGGATAGGTGGTTGAGTAAGATGATTGAGAGGGGCTTTGTTTTGGATAATGCTAGTTTTACTTTGATTATTAGTGTGTTTTGTGACAAGGGTTTTGTGAGTAGAGCGTCTTGGTGTTTCGATAAGATGAGTAAGATGGGGTTGAAGCCGAATTTGGTTAACTTTACGTCTTTGATACATGGGTTGTGCAAGAGGGGGAGTGTTAAGCAGGCGTTTGAGATGTTGGAGGAGATGGTTAGGAGAGGTTGGAAGCCGAATGTGTATACACATACCGCGTTGATTGATGGGTTGTGCAAGAAAGGTTGGACTGAAAGGGCGTTTAGGCTGTTTCTGAAGCTTGTCCGGAGTGATAGTTATAAGCCAAATGTGCATACATATACTGCGATGATCGGTGGATATTGCAAGGAGGATAAAATGAGCCGTGCGGAGATGTTGTTGAGCAGAATGAAGGAACAAGAATTAGTTCCTAACACCAATACGTATACTACTCTCGTTTATGGGCATTGCAAAGCTGGGAATTTTGAAAGAGCATATGAACTGATGGATGTAATGAGTGAAGAAGGATTTGCTCCGAATATATGTACATACAATGCAGTTATGGACAGTCTCTGTAAAAAGGGGAGGGTCCAAGAAGCTTATAAACTGATTAAGAAGGGCTTTCGCCAGGGATTGCAGGCTGATAGAGTCACGTATACCATTTTCATATCCGAGCACTGCAAACGGGGTGATATTAACAGTGCCCTAGCTTTTTTCAATAAGATGTTCAAGGCTGGATTGGAGCCTGATATACATTCATATACCACTTTGATTGCTGCCTTCTGCAGGCAAAAGAAAATGAAAGAAAGCGAGAAACTTTTCGAGGTAGCTGTAAGGCTTGGCTTGATTCCGGCCAAGGAGACCTATACATCCATGATATGTGGGTACTGTAGGGACGGAAATATTGCTTTGGCTGTAAAGTTCTTCCACAGGATGAGTGATCATGGTTGTTCCCCGGATAGTTTTACTTATGGTGCTCTAATAAGTGGGCTCTGCAAGGAGGAAAAACTTGACGAGGCTCGTAAGTTATATAATACCATGATGGACAAGGGGTTGTCACCCTGTGAAGTTACTAGGTTGACATTAGCTCACGAGTATTGCCAAAAAGATGACTATGCCACTGCTATGGTCATCTTAGATAGGCTAGACAAGAAGTTCTGGATCCGCACGGTTAACACATTGGTCAGGAAGCTTTGTTGTGAGAAGAAAGTGGGAATAGCAGCATTGTTCTTTCACAAATTAGTTGATAAGGATCAGAATGTGGATCGTGTGACATTGCAAGCATTTATGACTGCATGCTATGAGAGCAACAAGTATGCTCTTCTTTCGGATTTGACTGAGAGAATCTCTAAAGGAATTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

712

Amino Acids

80.46

Weight (kDa)

8.67

Isoelectric Point (pI)

35.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	194 - 239	4.9e-07	PPR repeat family
PPR_2	PF13041	263 - 309	1.3e-08	PPR repeat family
PPR_1	PF12854	291 - 324	1.5e-12	PPR repeat
PPR_2	PF13041	295 - 344	2.2e-18	PPR repeat family
PPR	PF01535	300 - 327	7.5e-07	PPR repeat
PPR_3	PF13812	319 - 379	1.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	327 - 355	1.9e-09	PPR repeat
PPR_2	PF13041	336 - 379	3.1e-09	PPR repeat family
PPR_long	PF17177	350 - 476	2.2e-09	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	357 - 460	1e-05	Fungal tetratrico peptide repeats
PPR_1	PF12854	364 - 395	1.4e-08	PPR repeat
PPR_2	PF13041	366 - 415	4.2e-15	PPR repeat family
PPR	PF01535	370 - 397	7e-07	PPR repeat
PPR_3	PF13812	390 - 446	2.9e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	399 - 429	6.5e-10	PPR repeat
PPR_2	PF13041	405 - 450	5e-12	PPR repeat family
PPR	PF01535	405 - 434	6.1e-06	PPR repeat
PPR_1	PF12854	433 - 462	5.2e-09	PPR repeat
PPR_2	PF13041	436 - 485	6.9e-13	PPR repeat family
PPR_1	PF12854	468 - 499	3.7e-08	PPR repeat
PPR_2	PF13041	472 - 520	2.7e-15	PPR repeat family
PPR_long	PF17177	479 - 638	1.4e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	503 - 532	4.2e-07	PPR repeat
PPR_2	PF13041	506 - 552	1.3e-08	PPR repeat family
PPR_3	PF13812	530 - 586	9.8e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	540 - 570	5.8e-08	PPR repeat
PPR	PF01535	545 - 574	1.7e-07	PPR repeat
PPR_2	PF13041	545 - 590	3e-16	PPR repeat family
PPR_1	PF12854	573 - 604	1.9e-11	PPR repeat
PPR	PF01535	579 - 609	6.7e-07	PPR repeat
PPR_2	PF13041	580 - 624	2.1e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19890
fragaria_vesca	FvH4_3g12270 FvH4_3g12270 FvH4_3g12270
malus_domestica	MD05G1251800.v1.1
prunus_persica	Prupe.4G111800_v2.0.a1
pyrus_communis	pycom05g22980 pycom10g19560
rosa_chinensis	RchiOBHm_Chr5g0020081 RchiOBHm_Chr7g0211421
rosa_laevigata	RLG00000032497
rosa_multiflora	Rmu_sc0021557.1_g000003
rosa_roxburghii	Rroxscaffold_1G00058120
rosa_rugosa	Rorug05G0056700
rosa_samantha	Rh5AG146800 Rh5BG146300 Rh5CG157900 Rh5DG145800 Rh6CG070300 Rh6DG067500 Rh7AG292200 Rh7CG312200 Rh7DG257000 Rh7DG263700
rosa_wichuraiana	Rw5G012970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1092, 1374
AccB7I	CCANNNNNTGG	1 cut(s)	475
AccI	GTMKAC	4 cut(s)	219, 998, 1217, 1427
AccII	CGCG	1 cut(s)	1010
AccIII	TCCGGA	1 cut(s)	1077
AciI	CCGC	8 cut(s)	132, 135, 138, 412, 725, 1008, 1160, 1936
AclWI	GGATC	4 cut(s)	1927, 1940, 2031, 2043
AcoI	YGGCCR	2 cut(s)	333, 1623
AcsI	RAATTY	4 cut(s)	18, 395, 886, 1252
AcuI	CTGAAG	1 cut(s)	1088
AdeI	CACNNNGTG	1 cut(s)	1835
AfaI	GTAC	3 cut(s)	574, 1318, 1658
AfiI	CCNNNNNNNGG	2 cut(s)	475, 1557
AflIII	ACRYGT	1 cut(s)	639
AgsI	TTSAA	8 cut(s)	41, 213, 250, 586, 880, 1259, 1489, 1501
AhdI	GACNNNNNGTC	1 cut(s)	652
AjnI	CCWGG	2 cut(s)	555, 1398
AjuI	GAANNNNNNNTTGG	2 cut(s)	872, 904
Alw21I	GWGCWC	2 cut(s)	1449, 1750
Alw26I	GTCTC	2 cut(s)	1346, 1625
AlwI	GGATC	4 cut(s)	1927, 1940, 2031, 2043
AlwNI	CAGNNNCTG	1 cut(s)	1344
Aor13HI	TCCGGA	1 cut(s)	1077
AoxI	GGCC	5 cut(s)	333, 342, 357, 559, 1623
ApeKI	GCWGC	3 cut(s)	527, 1547, 1991
ApoI	RAATTY	4 cut(s)	18, 395, 886, 1252
ArsI	GACNNNNNNTTYG	2 cut(s)	812, 844
Asp700I	GAANNNNTTC	4 cut(s)	477, 641, 1197, 1589
AspLEI	GCGC	1 cut(s)	431
AspS9I	GGNCC	5 cut(s)	348, 358, 465, 559, 1360
AsuC2I	CCSGG	2 cut(s)	346, 1727
AsuHPI	GGTGA	5 cut(s)	190, 249, 502, 1472, 1821
AsuII	TTCGAA	1 cut(s)	16
AvaII	GGWCC	3 cut(s)	348, 465, 1360
BaeGI	GKGCMC	1 cut(s)	1477
BaeI	ACNNNNGTAYC	2 cut(s)	902, 935
BamHI	GGATCC	1 cut(s)	1932
BanII	GRGCYC	1 cut(s)	1764
BauI	CACGAG	1 cut(s)	1862
BbsI	GAAGAC	1 cut(s)	246
Bbv12I	GWGCWC	2 cut(s)	1449, 1750
BbvCI	CCTCAGC	1 cut(s)	362
BbvI	GCAGC	3 cut(s)	514, 1534, 2003
BccI	CCATC	7 cut(s)	616, 671, 865, 961, 1013, 1270, 1807
BceAI	ACGGC	1 cut(s)	1140
BciT130I	CCWGG	2 cut(s)	557, 1400
BclI	TGATCA	1 cut(s)	1711
BcnI	CCSGG	2 cut(s)	346, 1727
BcoDI	GTCTC	2 cut(s)	1346, 1625
BfaI	CTAG	5 cut(s)	789, 1478, 1845, 1916, 2134
BfmI	CTRYAG	2 cut(s)	1555, 1660
BglI	GCCNNNNNGGC	1 cut(s)	341
BisI	GCNGC	6 cut(s)	135, 138, 413, 528, 1548, 1992
BlsI	GCNGC	6 cut(s)	136, 139, 414, 529, 1549, 1993
BmcAI	AGTACT	1 cut(s)	574
Bme1390I	CCNGG	4 cut(s)	346, 557, 1400, 1727
Bme18I	GGWCC	3 cut(s)	348, 465, 1360
BmeRI	GACNNNNNGTC	1 cut(s)	652
BmgT120I	GGNCC	5 cut(s)	348, 358, 465, 559, 1360
BmiI	GGNNCC	5 cut(s)	349, 467, 1361, 1515, 1934
BmrFI	CCNGG	4 cut(s)	346, 557, 1400, 1727
BmrI	ACTGGG	1 cut(s)	385
BmsI	GCATC	1 cut(s)	537
BmuI	ACTGGG	1 cut(s)	385
BpiI	GAAGAC	1 cut(s)	246
BplI	GAGNNNNNCTC	2 cut(s)	2102, 2134
Bpu10I	CCTNAGC	1 cut(s)	362
Bpu14I	TTCGAA	1 cut(s)	16
BpuEI	CTTGAG	1 cut(s)	68
BpuMI	CCSGG	2 cut(s)	346, 1727
BsaAI	YACGTR	2 cut(s)	1215, 1425
BsaBI	GATNNNNATC	1 cut(s)	2022
BsaI	GGTCTC	1 cut(s)	1625
BsaJI	CCNNGG	6 cut(s)	31, 351, 556, 1399, 1626, 1725
BsaWI	WCCGGW	1 cut(s)	1077
BsaXI	ACNNNNNCTCC	4 cut(s)	1072, 1102, 1154, 1184
Bsc4I	CCNNNNNNNGG	2 cut(s)	475, 1557
Bse1I	ACTGG	1 cut(s)	380
Bse3DI	GCAATG	4 cut(s)	454, 600, 1237, 2045
Bse8I	GATNNNNATC	1 cut(s)	2022
BseAI	TCCGGA	1 cut(s)	1077
BseBI	CCWGG	2 cut(s)	557, 1400
BseDI	CCNNGG	6 cut(s)	31, 351, 556, 1399, 1626, 1725
BseGI	GGATG	3 cut(s)	1285, 1640, 1710
BseJI	GATNNNNATC	1 cut(s)	2022
BseLI	CCNNNNNNNGG	2 cut(s)	475, 1557
BseMI	GCAATG	4 cut(s)	454, 600, 1237, 2045
BseMII	CTCAG	4 cut(s)	37, 376, 495, 2100
BseNI	ACTGG	1 cut(s)	380
BseRI	GAGGAG	4 cut(s)	118, 508, 678, 977
BseSI	GKGCMC	1 cut(s)	1477
BseXI	GCAGC	3 cut(s)	514, 1534, 2003
BseYI	CCCAGC	1 cut(s)	1247
Bsh1236I	CGCG	1 cut(s)	1010
BshFI	GGCC	5 cut(s)	335, 344, 359, 561, 1625
BsiHKAI	GWGCWC	2 cut(s)	1449, 1750
BsiSI	CCGG	4 cut(s)	345, 1078, 1622, 1727
BslFI	GGGAC	1 cut(s)	1679
BslI	CCNNNNNNNGG	2 cut(s)	475, 1557
BsmAI	GTCTC	2 cut(s)	1346, 1625
BsmFI	GGGAC	1 cut(s)	1679
BsnI	GGCC	5 cut(s)	335, 344, 359, 561, 1625
Bso31I	GGTCTC	1 cut(s)	1625
Bsp119I	TTCGAA	1 cut(s)	16
Bsp1286I	GDGCHC	4 cut(s)	1449, 1477, 1750, 1764
Bsp13I	TCCGGA	1 cut(s)	1077
Bsp1407I	TGTACA	1 cut(s)	1316
Bsp143I	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
Bsp19I	CCATGG	2 cut(s)	31, 351
BspACI	CCGC	8 cut(s)	132, 135, 138, 412, 725, 1008, 1160, 1936
BspANI	GGCC	5 cut(s)	335, 344, 359, 561, 1625
BspCNI	CTCAG	4 cut(s)	36, 375, 496, 2101
BspEI	TCCGGA	1 cut(s)	1077
BspFNI	CGCG	1 cut(s)	1010
BspLI	GGNNCC	5 cut(s)	349, 467, 1361, 1515, 1934
BspMAI	CTGCAG	1 cut(s)	1559
BspPI	GGATC	4 cut(s)	1927, 1940, 2031, 2043
BspQI	GCTCTTC	1 cut(s)	2097
BspT104I	TTCGAA	1 cut(s)	16
BspTNI	GGTCTC	1 cut(s)	1625
BsrDI	GCAATG	4 cut(s)	454, 600, 1237, 2045
BsrGI	TGTACA	1 cut(s)	1316
BsrI	ACTGG	1 cut(s)	380
BssECI	CCNNGG	6 cut(s)	31, 351, 556, 1399, 1626, 1725
BssMI	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
BssNAI	GTATAC	4 cut(s)	220, 999, 1218, 1428
BssSI	CACGAG	1 cut(s)	1862
BssT1I	CCWWGG	3 cut(s)	31, 351, 1626
Bst1107I	GTATAC	4 cut(s)	220, 999, 1218, 1428
Bst2BI	CACGAG	1 cut(s)	1862
Bst2UI	CCWGG	2 cut(s)	557, 1400
Bst4CI	ACNGT	6 cut(s)	590, 722, 1340, 1472, 1661, 1942
Bst6I	CTCTTC	3 cut(s)	18, 103, 2097
BstAPI	GCANNNNNTGC	1 cut(s)	606
BstAUI	TGTACA	1 cut(s)	1316
BstBAI	YACGTR	2 cut(s)	1215, 1425
BstBI	TTCGAA	1 cut(s)	16
BstC8I	GCNNGC	7 cut(s)	333, 607, 945, 1410, 1559, 2052, 2068
BstDEI	CTNAG	5 cut(s)	23, 362, 504, 1906, 2109
BstDSI	CCRYGG	2 cut(s)	31, 351
BstEII	GGTNACC	1 cut(s)	196
BstENI	CCTNNNNNAGG	1 cut(s)	1555
BstF5I	GGATG	3 cut(s)	1285, 1640, 1710
BstFNI	CGCG	1 cut(s)	1010
BstHHI	GCGC	1 cut(s)	431
BstKTI	GATC	5 cut(s)	1125, 1714, 1935, 2026, 2038
BstMAI	GTCTC	2 cut(s)	1346, 1625
BstMBI	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
BstMWI	GCNNNNNNNGC	6 cut(s)	55, 332, 341, 606, 1102, 2076
BstNI	CCWGG	2 cut(s)	557, 1400
BstNSI	RCATGY	1 cut(s)	2070
BstPI	GGTNACC	1 cut(s)	196
BstSCI	CCNGG	4 cut(s)	344, 555, 1398, 1725
BstSFI	CTRYAG	2 cut(s)	1555, 1660
BstSLI	GKGCMC	1 cut(s)	1477
BstSNI	TACGTA	1 cut(s)	1215
BstUI	CGCG	1 cut(s)	1010
BstV1I	GCAGC	3 cut(s)	514, 1534, 2003
BstV2I	GAAGAC	1 cut(s)	246
BstX2I	RGATCY	1 cut(s)	1932
BstXI	CCANNNNNNTGG	1 cut(s)	1897
BstYI	RGATCY	1 cut(s)	1932
BstZ17I	GTATAC	4 cut(s)	220, 999, 1218, 1428
BsuRI	GGCC	5 cut(s)	335, 344, 359, 561, 1625
BtgI	CCRYGG	2 cut(s)	31, 351
BtgZI	GCGATG	1 cut(s)	1133
BtsCI	GGATG	3 cut(s)	1285, 1640, 1710
BtsI	GCAGTG	2 cut(s)	1447, 1890
BtsIMutI	CAGTG	5 cut(s)	105, 488, 1447, 1477, 1890
Cac8I	GCNNGC	7 cut(s)	333, 607, 945, 1410, 1559, 2052, 2068
CaiI	CAGNNNCTG	1 cut(s)	1344
CfoI	GCGC	1 cut(s)	431
Cfr13I	GGNCC	5 cut(s)	348, 358, 465, 559, 1360
CseI	GACGC	1 cut(s)	831
Csp6I	GTAC	3 cut(s)	573, 1317, 1657
CspCI	CAANNNNNGTGG	2 cut(s)	1525, 1560
CviQI	GTAC	3 cut(s)	573, 1317, 1657
DdeI	CTNAG	5 cut(s)	23, 362, 504, 1906, 2109
DpnI	GATC	5 cut(s)	1124, 1713, 1934, 2025, 2037
DpnII	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
DraI	TTTAAA	1 cut(s)	520
DraIII	CACNNNGTG	1 cut(s)	1835
DriI	GACNNNNNGTC	1 cut(s)	652
EaeI	YGGCCR	2 cut(s)	333, 1623
Eam1104I	CTCTTC	3 cut(s)	18, 103, 2097
Eam1105I	GACNNNNNGTC	1 cut(s)	652
EarI	CTCTTC	3 cut(s)	18, 103, 2097
EciI	GGCGGA	1 cut(s)	121
Eco105I	TACGTA	1 cut(s)	1215
Eco130I	CCWWGG	3 cut(s)	31, 351, 1626
Eco24I	GRGCYC	1 cut(s)	1764
Eco31I	GGTCTC	1 cut(s)	1625
Eco47I	GGWCC	3 cut(s)	348, 465, 1360
Eco57I	CTGAAG	1 cut(s)	1088
Eco91I	GGTNACC	1 cut(s)	196
EcoNI	CCTNNNNNAGG	1 cut(s)	1555
EcoO109I	RGGNCCY	2 cut(s)	465, 559
EcoO65I	GGTNACC	1 cut(s)	196
EcoRI	GAATTC	1 cut(s)	18
EcoRII	CCWGG	2 cut(s)	555, 1398
EcoT14I	CCWWGG	3 cut(s)	31, 351, 1626
EcoT38I	GRGCYC	1 cut(s)	1764
ErhI	CCWWGG	3 cut(s)	31, 351, 1626
FalI	AAGNNNNNCTT	2 cut(s)	1376, 1408
FaqI	GGGAC	1 cut(s)	1679
FauNDI	CATATG	1 cut(s)	1267
FbaI	TGATCA	1 cut(s)	1711
FblI	GTMKAC	4 cut(s)	219, 998, 1217, 1427
Fnu4HI	GCNGC	6 cut(s)	135, 138, 413, 528, 1548, 1992
FokI	GGATG	3 cut(s)	1292, 1627, 1717
FriOI	GRGCYC	1 cut(s)	1764
Fsp4HI	GCNGC	6 cut(s)	135, 138, 413, 528, 1548, 1992
FspBI	CTAG	5 cut(s)	789, 1478, 1845, 1916, 2134
GlaI	GCGC	1 cut(s)	430
GluI	GCNGC	6 cut(s)	135, 138, 413, 528, 1548, 1992
GsaI	CCCAGC	1 cut(s)	1251
HaeIII	GGCC	5 cut(s)	335, 344, 359, 561, 1625
HapII	CCGG	4 cut(s)	345, 1078, 1622, 1727
HgaI	GACGC	1 cut(s)	831
HhaI	GCGC	1 cut(s)	431
Hin6I	GCGC	1 cut(s)	429
HinP1I	GCGC	1 cut(s)	429
HincII	GTYRAC	3 cut(s)	895, 1851, 1945
HindII	GTYRAC	3 cut(s)	895, 1851, 1945
HindIII	AAGCTT	3 cut(s)	1070, 1368, 1961
HinfI	GANTC	8 cut(s)	110, 170, 182, 284, 290, 1419, 1618, 2115
HpaI	GTTAAC	2 cut(s)	895, 1945
HpaII	CCGG	4 cut(s)	345, 1078, 1622, 1727
HphI	GGTGA	5 cut(s)	190, 249, 502, 1472, 1821
Hpy166II	GTNNAC	7 cut(s)	220, 895, 999, 1218, 1428, 1851, 1945
Hpy188III	TCNNGA	7 cut(s)	85, 544, 674, 1078, 1862, 1930, 1957
Hpy8I	GTNNAC	7 cut(s)	220, 895, 999, 1218, 1428, 1851, 1945
Hpy99I	CGWCG	2 cut(s)	341, 650
HpyAV	CCTTC	5 cut(s)	112, 1177, 1289, 1381, 1561
HpyCH4III	ACNGT	6 cut(s)	590, 722, 1340, 1472, 1661, 1942
HpyCH4IV	ACGT	4 cut(s)	639, 902, 1214, 1424
HpyF10VI	GCNNNNNNNGC	6 cut(s)	55, 332, 341, 606, 1102, 2076
HpyF3I	CTNAG	5 cut(s)	23, 362, 504, 1906, 2109
HpySE526I	ACGT	4 cut(s)	639, 902, 1214, 1424
HspAI	GCGC	1 cut(s)	429
Kpn2I	TCCGGA	1 cut(s)	1077
Ksp22I	TGATCA	1 cut(s)	1711
KspAI	GTTAAC	2 cut(s)	895, 1945
Kzo9I	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
LguI	GCTCTTC	1 cut(s)	2097
LmnI	GCTCC	2 cut(s)	1309, 1513
Lsp1109I	GCAGC	3 cut(s)	514, 1534, 2003
LweI	GCATC	1 cut(s)	537
MaeI	CTAG	5 cut(s)	789, 1478, 1845, 1916, 2134
MaeII	ACGT	4 cut(s)	639, 902, 1214, 1424
MaeIII	GTNAC	6 cut(s)	196, 818, 1420, 1827, 1840, 2041
MalI	GATC	5 cut(s)	1124, 1713, 1934, 2025, 2037
MboI	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
MboII	GAAGA	8 cut(s)	5, 90, 246, 415, 1304, 1687, 1987, 2084
MfeI	CAATTG	1 cut(s)	381
MflI	RGATCY	1 cut(s)	1932
MhlI	GDGCHC	4 cut(s)	1449, 1477, 1750, 1764
MluCI	AATT	9 cut(s)	18, 381, 395, 454, 886, 1193, 1252, 2009, 2127
MlyI	GAGTC	3 cut(s)	104, 284, 1428
MmeI	TCCRAC	3 cut(s)	939, 962, 1019
MroI	TCCGGA	1 cut(s)	1077
MroXI	GAANNNNTTC	4 cut(s)	477, 641, 1197, 1589
MseI	TTAA	7 cut(s)	519, 690, 894, 939, 1383, 1467, 1944
MslI	CAYNNNNRTG	1 cut(s)	1895
MspI	CCGG	4 cut(s)	345, 1078, 1622, 1727
MspR9I	CCNGG	4 cut(s)	346, 557, 1400, 1727
MunI	CAATTG	1 cut(s)	381
MvaI	CCWGG	2 cut(s)	557, 1400
MvnI	CGCG	1 cut(s)	1010
MwoI	GCNNNNNNNGC	6 cut(s)	55, 332, 341, 606, 1102, 2076
NciI	CCSGG	2 cut(s)	346, 1727
NcoI	CCATGG	2 cut(s)	31, 351
NdeI	CATATG	1 cut(s)	1267
NdeII	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
NlaIV	GGNNCC	5 cut(s)	349, 467, 1361, 1515, 1934
NmuCI	GTSAC	5 cut(s)	196, 818, 1420, 1827, 2041
NspI	RCATGY	1 cut(s)	2070
NspV	TTCGAA	1 cut(s)	16
PaeI	GCATGC	1 cut(s)	2070
PciSI	GCTCTTC	1 cut(s)	2097
PcsI	WCGNNNNNNNCGW	3 cut(s)	62, 315, 645
PdmI	GAANNNNTTC	4 cut(s)	477, 641, 1197, 1589
PfeI	GAWTC	5 cut(s)	170, 182, 284, 1618, 2115
PflMI	CCANNNNNTGG	1 cut(s)	475
PkrI	GCNGC	6 cut(s)	136, 139, 414, 529, 1549, 1993
PleI	GAGTC	3 cut(s)	104, 284, 1427
PpsI	GAGTC	3 cut(s)	104, 284, 1427
Ppu21I	YACGTR	2 cut(s)	1215, 1425
PpuMI	RGGWCCY	1 cut(s)	465
PsiI	TTATAA	2 cut(s)	1092, 1374
Psp5II	RGGWCCY	1 cut(s)	465
Psp6I	CCWGG	2 cut(s)	555, 1398
PspEI	GGTNACC	1 cut(s)	196
PspFI	CCCAGC	1 cut(s)	1247
PspGI	CCWGG	2 cut(s)	555, 1398
PspN4I	GGNNCC	5 cut(s)	349, 467, 1361, 1515, 1934
PspPI	GGNCC	5 cut(s)	348, 358, 465, 559, 1360
PspPPI	RGGWCCY	1 cut(s)	465
PstI	CTGCAG	1 cut(s)	1559
PstNI	CAGNNNCTG	1 cut(s)	1344
PsuI	RGATCY	1 cut(s)	1932
RsaI	GTAC	3 cut(s)	574, 1318, 1658
RsaNI	GTAC	3 cut(s)	573, 1317, 1657
RseI	CAYNNNNRTG	1 cut(s)	1895
SapI	GCTCTTC	1 cut(s)	2097
SaqAI	TTAA	7 cut(s)	519, 690, 894, 939, 1383, 1467, 1944
SatI	GCNGC	6 cut(s)	135, 138, 413, 528, 1548, 1992
Sau3AI	GATC	5 cut(s)	1122, 1711, 1932, 2023, 2035
Sau96I	GGNCC	5 cut(s)	348, 358, 465, 559, 1360
ScaI	AGTACT	1 cut(s)	574
SchI	GAGTC	3 cut(s)	104, 284, 1428
ScrFI	CCNGG	4 cut(s)	346, 557, 1400, 1727
SduI	GDGCHC	4 cut(s)	1449, 1477, 1750, 1764
SfaNI	GCATC	1 cut(s)	537
SfcI	CTRYAG	2 cut(s)	1555, 1660
SfiI	GGCCNNNNNGGCC	1 cut(s)	341
SfuI	TTCGAA	1 cut(s)	16
SinI	GGWCC	3 cut(s)	348, 465, 1360
SmiMI	CAYNNNNRTG	1 cut(s)	1895
SmlI	CTYRAG	1 cut(s)	83
SmoI	CTYRAG	1 cut(s)	83
SnaBI	TACGTA	1 cut(s)	1215
SphI	GCATGC	1 cut(s)	2070
Sse9I	AATT	9 cut(s)	18, 381, 395, 454, 886, 1193, 1252, 2009, 2127
SsiI	CCGC	8 cut(s)	132, 135, 138, 412, 725, 1008, 1160, 1936
SspI	AATATT	1 cut(s)	1675
SspMI	CTAG	5 cut(s)	789, 1478, 1845, 1916, 2134
StyD4I	CCNGG	4 cut(s)	344, 555, 1398, 1725
StyI	CCWWGG	3 cut(s)	31, 351, 1626
TaaI	ACNGT	6 cut(s)	590, 722, 1340, 1472, 1661, 1942
TaiI	ACGT	4 cut(s)	642, 905, 1217, 1427
TaqI	TCGA	9 cut(s)	16, 65, 400, 437, 543, 645, 715, 855, 1593
TasI	AATT	9 cut(s)	18, 381, 395, 454, 886, 1193, 1252, 2009, 2127
TatI	WGTACW	2 cut(s)	572, 1316
TauI	GCSGC	3 cut(s)	137, 140, 415
TfiI	GAWTC	5 cut(s)	170, 182, 284, 1618, 2115
Tru1I	TTAA	7 cut(s)	519, 690, 894, 939, 1383, 1467, 1944
Tru9I	TTAA	7 cut(s)	519, 690, 894, 939, 1383, 1467, 1944
TscAI	CASTG	5 cut(s)	112, 488, 1454, 1477, 1897
TseFI	GTSAC	5 cut(s)	196, 818, 1420, 1827, 2041
TseI	GCWGC	3 cut(s)	527, 1547, 1991
Tsp45I	GTSAC	5 cut(s)	196, 818, 1420, 1827, 2041
TspDTI	ATGAA	7 cut(s)	132, 486, 1196, 1284, 1426, 1518, 1586
TspGWI	ACGGA	2 cut(s)	307, 1683
TspRI	CASTG	5 cut(s)	112, 488, 1454, 1477, 1897
Van91I	CCANNNNNTGG	1 cut(s)	475
VpaK11BI	GGWCC	3 cut(s)	348, 465, 1360
XagI	CCTNNNNNAGG	1 cut(s)	1555
XapI	RAATTY	4 cut(s)	18, 395, 886, 1252
XceI	RCATGY	1 cut(s)	2070
XcmI	CCANNNNNNNNNTGG	1 cut(s)	319
XmiI	GTMKAC	4 cut(s)	219, 998, 1217, 1427
XmnI	GAANNNNTTC	4 cut(s)	477, 641, 1197, 1589
XspI	CTAG	5 cut(s)	789, 1478, 1845, 1916, 2134
ZrmI	AGTACT	1 cut(s)	574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.