MD05G1267400.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013793
Physical Location & Seq
Forward (+)
40268162 .. 40269760
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1267400.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGAAGGCCTTAGAAAATGCAATACTATTATGTGCAGCATTTCCAATCTTTAGTTTGATTTCAGTGTCATGTACCAATTCATCCTCAGTTGAATACAATTTTCTCCAATGCCTTTCAAATCATTCTCATTCCTCAAATCCAATCTCTGAGGCACTTTTCACCCCCAAAAGTGCCTCTTTTTCTTCTATTCTGCAGTCATATGTGAGAAATCTCAGATTTATGACTCCCACAATCCCGAAACCGATGGTAATTATTGCAGCCAAGCACGAATCCCATGTTCAAGCCACAGTCACATGTGCCAAAACCCTTGGCTTGGAAATTAGAATTAGAAGTGGCGGACATGATTATGAAGGCGTCTCCTATGTATCCAGAGACGCAAAAGATTTCATCCTTCTCGACATGTTTAATCTCCGGTCCATCTACGTTGATTTGGCAGATGAAAGTGCATGGGTTCAATCTGGGGCTATACTTGGTGAAATTTTCTATGCAATTTTTAAGAAAAGCAAGTACCATGCCTTCCCTGCTGGGGTCTGTCCTACAATTGGTGCTGGTGGGCATTTCACAGGAGGTGGCTATGGTAACATGATGAGAAAATATGGGCTTTCCTCTGATAACATTGTTGATGCAAAAATAGTAAATGTAAATGGTACAATTCTTGATAGAAAATCAATGCGGGAAGATCTATTTTGGGCCATTAGAGGAGGTGGTGGATCAAGTTTTGGGGTCATTCTTGCTTGGAAAATCAAGCTGGTCCGGGTTCCGGCCCTGGTAACGGTGTTTGATGTCAAAAAAACATTAGCAGAAGGTGCAACAGACATTCTTGTGAAATGGCAACAAATTGCTGATAAGCTAGATAATGATCTCTTCATAAGAGCCGTTCCTAAACCAGTCAATCGAACAATTGAGGTTTCTTTTGTTTCCTTGTTTTTGGGGAACTCCGATAGACTTTTGAAGCTGATGAACCAAAGCTTTCCTCAGTTGGGTTTGGAGAAAAAAGATTGCATAGAGATGAGTTGGATTCAATCGGTTTTGTTTTGGGCTGACTACAGAAATGGAACTACTCCTCCAGATGTTCTGCTCAACAGGGTCCCCAGCTCAGGAAAAGTGTTCTTGAAGCGCAAATCTGATTACGTGAAAGAACCAATTTCAAAGACGGATTTGGAAGCCTTGTGGAAGGTGATGATTGAGATTGGAGAAGTTATGATGCTGTGGAATCCTTATGGAGGAAAAATGAGTGAGATTCCCGAGACAGCTACTCCATTTCCTCATAGAGCAGGCAACCTATTCAAGATTCAGTATTCAGTTAACTGGAAGGAGGAAGGGGTTGAGGCCACCAAAAAGTACTTGCGTTTGATGAAAAAACTTTTTGAAGCAATGACTCCTCATGTGTCCAAGAATCCAAGGGAGGCTTTTTACAACTATCGTGACCTTGACGTTGGTACGAATTTAAAAGGTGGATACACTGAAGCAAAAGTGTATGGAACTAAGTACTTCAAGGGTAATTTTGAAAGATTAGTGCGTATTAAGACAGCAGTGGATCCTGAGAATTTTTTCAGAAATGAACAAAGTATTCCAACTAATAAATCTATGGAACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009505 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0030312 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

533

Amino Acids

59.73

Weight (kDa)

9.2

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 82 - 219 8.7e-21 FAD binding domain
BBE PF08031 470 - 525 6.1e-20 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013793)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30710 AT1G30720 AT1G30730 AT1G34575 AT2G34810
fragaria_vesca FvH4_3g11041
malus_domestica MD05G1267400.v1.1 MD05G1268700.v1.1
prunus_persica Prupe.4G098800_v2.0.a1
pyrus_communis pycom05g25040
rosa_chinensis RchiOBHm_Chr5g0017761
rosa_laevigata RLG00000032313
rosa_multiflora Rmu_sc0009555.1_g000004
rosa_roxburghii Rroxscaffold_1G00059720 Rroxscaffold_1G00059730
rosa_rugosa Rorug05G0038500
rosa_wichuraiana Rw5G011420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 336, 673
AclWI GGATC 3 cut(s) 718, 1532, 1545
AcsI RAATTY 3 cut(s) 477, 1444, 1546
AcuI CTGAAG 1 cut(s) 1485
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 6 cut(s) 73, 509, 649, 1343, 1441, 1490
AfiI CCNNNNNNNGG 8 cut(s) 313, 526, 542, 760, 772, 980, 1097, 1223
AflIII ACRYGT 2 cut(s) 293, 399
AjnI CCWGG 1 cut(s) 765
AloI GAACNNNNNNTCC 2 cut(s) 1048, 1080
AluBI AGCT 6 cut(s) 748, 850, 955, 969, 1095, 1253
AluI AGCT 6 cut(s) 748, 850, 955, 969, 1095, 1253
Alw26I GTCTC 3 cut(s) 361, 366, 1241
AlwI GGATC 3 cut(s) 718, 1532, 1545
Ama87I CYCGRG 1 cut(s) 1244
AoxI GGCC 4 cut(s) 6, 690, 762, 1329
ApeKI GCWGC 2 cut(s) 35, 257
ApoI RAATTY 3 cut(s) 477, 1444, 1546
Asp700I GAANNNNTTC 1 cut(s) 1550
AspLEI GCGC 1 cut(s) 1119
AspS9I GGNCC 5 cut(s) 414, 690, 751, 763, 1087
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 3 cut(s) 151, 485, 1189
AvaI CYCGRG 1 cut(s) 1244
AvaII GGWCC 3 cut(s) 414, 751, 1087
BamHI GGATCC 1 cut(s) 1537
BarI GAAGNNNNNNTAC 2 cut(s) 500, 532
BbvI GCAGC 2 cut(s) 47, 269
BccI CCATC 2 cut(s) 238, 425
BceAI ACGGC 1 cut(s) 860
BciT130I CCWGG 1 cut(s) 767
BciVI GTATCC 2 cut(s) 376, 1451
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 3 cut(s) 361, 366, 1241
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 2 cut(s) 191, 1045
BfuI GTATCC 2 cut(s) 376, 1451
BglII AGATCT 1 cut(s) 679
BisI GCNGC 2 cut(s) 36, 258
BlsI GCNGC 2 cut(s) 37, 259
BmcAI AGTACT 2 cut(s) 1343, 1490
Bme1390I CCNGG 2 cut(s) 755, 767
Bme18I GGWCC 3 cut(s) 414, 751, 1087
BmeT110I CYCGRG 1 cut(s) 1244
BmgT120I GGNCC 5 cut(s) 414, 690, 751, 763, 1087
BmiI GGNNCC 4 cut(s) 759, 1088, 1089, 1539
BmrFI CCNGG 2 cut(s) 755, 767
BmsI GCATC 2 cut(s) 613, 1194
BpmI CTGGAG 1 cut(s) 1050
Bpu10I CCTNAGC 1 cut(s) 1096
BpuMI CCSGG 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 1132
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 307, 765, 1400
BsaWI WCCGGW 1 cut(s) 411
BsaXI ACNNNNNCTCC 4 cut(s) 1048, 1078, 1240, 1270
Bsc4I CCNNNNNNNGG 8 cut(s) 313, 526, 542, 760, 772, 980, 1097, 1223
Bse1I ACTGG 2 cut(s) 887, 1313
Bse3DI GCAATG 1 cut(s) 1380
Bse8I GATNNNNATC 1 cut(s) 858
BseBI CCWGG 1 cut(s) 767
BseDI CCNNGG 3 cut(s) 307, 765, 1400
BseGI GGATG 2 cut(s) 80, 387
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 8 cut(s) 313, 526, 542, 760, 772, 980, 1097, 1223
BseMI GCAATG 1 cut(s) 1380
BseMII CTCAG 6 cut(s) 99, 138, 226, 989, 1110, 1533
BseNI ACTGG 2 cut(s) 887, 1313
BseRI GAGGAG 3 cut(s) 714, 1053, 1371
BseXI GCAGC 2 cut(s) 47, 269
BseYI CCCAGC 2 cut(s) 524, 1091
BsgI GTGCAG 1 cut(s) 54
BshFI GGCC 4 cut(s) 8, 692, 764, 1331
BsiHKCI CYCGRG 1 cut(s) 1244
BsiSI CCGG 3 cut(s) 412, 754, 761
BslFI GGGAC 1 cut(s) 1073
BslI CCNNNNNNNGG 8 cut(s) 313, 526, 542, 760, 772, 980, 1097, 1223
BsmAI GTCTC 3 cut(s) 361, 366, 1241
BsmBI CGTCTC 2 cut(s) 361, 366
BsmFI GGGAC 1 cut(s) 1073
BsnI GGCC 4 cut(s) 8, 692, 764, 1331
BsoBI CYCGRG 1 cut(s) 1244
Bsp143I GATC 4 cut(s) 679, 710, 859, 1537
BspACI CCGC 2 cut(s) 336, 673
BspANI GGCC 4 cut(s) 8, 692, 764, 1331
BspCNI CTCAG 6 cut(s) 98, 139, 225, 988, 1109, 1534
BspLI GGNNCC 4 cut(s) 759, 1088, 1089, 1539
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 3 cut(s) 718, 1532, 1545
BsrDI GCAATG 1 cut(s) 1380
BsrI ACTGG 2 cut(s) 887, 1313
BssECI CCNNGG 3 cut(s) 307, 765, 1400
BssMI GATC 4 cut(s) 679, 710, 859, 1537
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 307, 1400
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 2 cut(s) 289, 775
Bst6I CTCTTC 1 cut(s) 869
BstACI GRCGYC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 1132
BstC8I GCNNGC 1 cut(s) 1276
BstDEI CTNAG 8 cut(s) 10, 85, 147, 212, 975, 1096, 1485, 1542
BstENI CCTNNNNNAGG 1 cut(s) 1221
BstF5I GGATG 2 cut(s) 80, 387
BstHHI GCGC 1 cut(s) 1119
BstKTI GATC 4 cut(s) 682, 713, 862, 1540
BstMAI GTCTC 3 cut(s) 361, 366, 1241
BstMBI GATC 4 cut(s) 679, 710, 859, 1537
BstMWI GCNNNNNNNGC 2 cut(s) 521, 806
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 2 cut(s) 297, 403
BstSCI CCNGG 2 cut(s) 753, 765
BstSFI CTRYAG 2 cut(s) 191, 1045
BstV1I GCAGC 2 cut(s) 47, 269
BstX2I RGATCY 2 cut(s) 679, 1537
BstYI RGATCY 2 cut(s) 679, 1537
BsuI GTATCC 2 cut(s) 376, 1451
BsuRI GGCC 4 cut(s) 8, 692, 764, 1331
BtsCI GGATG 2 cut(s) 80, 387
BtsI GCAGTG 1 cut(s) 1539
BtsIMutI CAGTG 3 cut(s) 69, 1461, 1539
Cac8I GCNNGC 1 cut(s) 1276
CfoI GCGC 1 cut(s) 1119
Cfr13I GGNCC 5 cut(s) 414, 690, 751, 763, 1087
CseI GACGC 2 cut(s) 343, 383
Csp6I GTAC 6 cut(s) 72, 508, 648, 1342, 1440, 1489
CviAII CATG 9 cut(s) 69, 275, 294, 341, 400, 447, 512, 583, 1385
CviQI GTAC 6 cut(s) 72, 508, 648, 1342, 1440, 1489
DdeI CTNAG 8 cut(s) 10, 85, 147, 212, 975, 1096, 1485, 1542
DpnI GATC 4 cut(s) 681, 712, 861, 1539
DpnII GATC 4 cut(s) 679, 710, 859, 1537
DraI TTTAAA 1 cut(s) 1449
Eam1104I CTCTTC 1 cut(s) 869
EarI CTCTTC 1 cut(s) 869
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 307, 1400
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 414, 751, 1087
Eco57I CTGAAG 1 cut(s) 1485
Eco88I CYCGRG 1 cut(s) 1244
EcoNI CCTNNNNNAGG 1 cut(s) 1221
EcoO109I RGGNCCY 1 cut(s) 1087
EcoRII CCWGG 1 cut(s) 765
EcoT14I CCWWGG 2 cut(s) 307, 1400
ErhI CCWWGG 2 cut(s) 307, 1400
Esp3I CGTCTC 2 cut(s) 361, 366
FaeI CATG 9 cut(s) 72, 278, 297, 344, 403, 450, 515, 586, 1388
FalI AAGNNNNNCTT 4 cut(s) 160, 192, 1393, 1425
FaqI GGGAC 1 cut(s) 1073
FatI CATG 9 cut(s) 68, 274, 293, 340, 399, 446, 511, 582, 1384
FauI CCCGC 1 cut(s) 666
FauNDI CATATG 1 cut(s) 199
Fnu4HI GCNGC 2 cut(s) 36, 258
FokI GGATG 2 cut(s) 67, 374
Fsp4HI GCNGC 2 cut(s) 36, 258
FspBI CTAG 1 cut(s) 851
GlaI GCGC 1 cut(s) 1118
GluI GCNGC 2 cut(s) 36, 258
GsaI CCCAGC 2 cut(s) 528, 1095
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 4 cut(s) 8, 692, 764, 1331
HapII CCGG 3 cut(s) 412, 754, 761
HgaI GACGC 2 cut(s) 343, 383
HhaI GCGC 1 cut(s) 1119
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 9 cut(s) 72, 278, 297, 344, 403, 450, 515, 586, 1388
Hin6I GCGC 1 cut(s) 1117
HinP1I GCGC 1 cut(s) 1117
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HindIII AAGCTT 1 cut(s) 967
HinfI GANTC 8 cut(s) 223, 269, 1018, 1213, 1240, 1291, 1378, 1396
HpaI GTTAAC 1 cut(s) 1306
HpaII CCGG 3 cut(s) 412, 754, 761
HphI GGTGA 3 cut(s) 151, 485, 1189
Hpy166II GTNNAC 1 cut(s) 1306
Hpy188I TCNGA 6 cut(s) 148, 215, 610, 940, 1126, 1556
Hpy8I GTNNAC 1 cut(s) 1306
HpyAV CCTTC 7 cut(s) 344, 401, 526, 797, 1168, 1306, 1313
HpyCH4III ACNGT 2 cut(s) 289, 775
HpyCH4IV ACGT 3 cut(s) 423, 1131, 1434
HpyCH4V TGCA 9 cut(s) 20, 35, 193, 257, 446, 488, 626, 809, 1002
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 806
HpyF3I CTNAG 8 cut(s) 10, 85, 147, 212, 975, 1096, 1485, 1542
HpySE526I ACGT 3 cut(s) 423, 1131, 1434
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 9 cut(s) 72, 278, 297, 344, 403, 450, 515, 586, 1388
HspAI GCGC 1 cut(s) 1117
KflI GGGWCCC 1 cut(s) 1087
KspAI GTTAAC 1 cut(s) 1306
Kzo9I GATC 4 cut(s) 679, 710, 859, 1537
Lsp1109I GCAGC 2 cut(s) 47, 269
LweI GCATC 2 cut(s) 613, 1194
MaeI CTAG 1 cut(s) 851
MaeII ACGT 3 cut(s) 423, 1131, 1434
MaeIII GTNAC 4 cut(s) 289, 578, 769, 1424
MalI GATC 4 cut(s) 681, 712, 861, 1539
MboI GATC 4 cut(s) 679, 710, 859, 1537
MboII GAAGA 3 cut(s) 174, 689, 856
MfeI CAATTG 2 cut(s) 540, 900
MflI RGATCY 2 cut(s) 679, 1537
MlyI GAGTC 2 cut(s) 217, 1372
MmeI TCCRAC 2 cut(s) 995, 1598
MroXI GAANNNNTTC 1 cut(s) 1550
MseI TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
MslI CAYNNNNRTG 3 cut(s) 345, 821, 1007
MspI CCGG 3 cut(s) 412, 754, 761
MspR9I CCNGG 2 cut(s) 755, 767
MunI CAATTG 2 cut(s) 540, 900
MvaI CCWGG 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 521, 806
NciI CCSGG 1 cut(s) 755
NdeI CATATG 1 cut(s) 199
NdeII GATC 4 cut(s) 679, 710, 859, 1537
NlaIII CATG 9 cut(s) 72, 278, 297, 344, 403, 450, 515, 586, 1388
NlaIV GGNNCC 4 cut(s) 759, 1088, 1089, 1539
NmuCI GTSAC 2 cut(s) 289, 1424
NspI RCATGY 2 cut(s) 297, 403
PceI AGGCCT 1 cut(s) 8
PciI ACATGT 2 cut(s) 293, 399
PdmI GAANNNNTTC 1 cut(s) 1550
PfeI GAWTC 6 cut(s) 269, 1018, 1213, 1240, 1291, 1396
PkrI GCNGC 2 cut(s) 37, 259
PleI GAGTC 2 cut(s) 217, 1372
PpsI GAGTC 2 cut(s) 217, 1372
Ppu21I YACGTR 1 cut(s) 1132
PpuMI RGGWCCY 1 cut(s) 1087
PscI ACATGT 2 cut(s) 293, 399
Psp5II RGGWCCY 1 cut(s) 1087
Psp6I CCWGG 1 cut(s) 765
PspFI CCCAGC 2 cut(s) 524, 1091
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 759, 1088, 1089, 1539
PspPI GGNCC 5 cut(s) 414, 690, 751, 763, 1087
PspPPI RGGWCCY 1 cut(s) 1087
PstI CTGCAG 1 cut(s) 195
PsuI RGATCY 2 cut(s) 679, 1537
RsaI GTAC 6 cut(s) 73, 509, 649, 1343, 1441, 1490
RsaNI GTAC 6 cut(s) 72, 508, 648, 1342, 1440, 1489
RseI CAYNNNNRTG 3 cut(s) 345, 821, 1007
SaqAI TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
SatI GCNGC 2 cut(s) 36, 258
Sau3AI GATC 4 cut(s) 679, 710, 859, 1537
Sau96I GGNCC 5 cut(s) 414, 690, 751, 763, 1087
ScaI AGTACT 2 cut(s) 1343, 1490
SchI GAGTC 2 cut(s) 217, 1372
ScrFI CCNGG 2 cut(s) 755, 767
SfaNI GCATC 2 cut(s) 613, 1194
SfcI CTRYAG 2 cut(s) 191, 1045
SinI GGWCC 3 cut(s) 414, 751, 1087
SmiMI CAYNNNNRTG 3 cut(s) 345, 821, 1007
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 2 cut(s) 336, 673
SspMI CTAG 1 cut(s) 851
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 753, 765
StyI CCWWGG 2 cut(s) 307, 1400
TaaI ACNGT 2 cut(s) 289, 775
TaiI ACGT 3 cut(s) 426, 1134, 1437
TaqI TCGA 2 cut(s) 396, 895
TatI WGTACW 2 cut(s) 1341, 1488
TfiI GAWTC 6 cut(s) 269, 1018, 1213, 1240, 1291, 1396
Tru1I TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
Tru9I TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
TscAI CASTG 3 cut(s) 69, 1468, 1539
TseFI GTSAC 2 cut(s) 289, 1424
TseI GCWGC 2 cut(s) 35, 257
Tsp45I GTSAC 2 cut(s) 289, 1424
TspDTI ATGAA 9 cut(s) 17, 69, 363, 376, 453, 856, 974, 1370, 1575
TspGWI ACGGA 1 cut(s) 1169
TspRI CASTG 3 cut(s) 69, 1468, 1539
VpaK11BI GGWCC 3 cut(s) 414, 751, 1087
XagI CCTNNNNNAGG 1 cut(s) 1221
XapI RAATTY 3 cut(s) 477, 1444, 1546
XceI RCATGY 2 cut(s) 297, 403
XmnI GAANNNNTTC 1 cut(s) 1550
XspI CTAG 1 cut(s) 851
ZrmI AGTACT 2 cut(s) 1343, 1490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.