Rroxscaffold_1G00059730

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013793
Physical Location & Seq
Forward (+)
81792361 .. 81794868
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059730.1

Sequence Viewer

Length: 897 bp
ATGCTGTTACCAGGGAATGGAAGCAGAGAAGGTGAAAAGACAATCACGGCCACTTTTGTTTCCTTGTTCCTGGGGGATTCTGTAAGGCTTCTGAGGCTGCTGAATCAAAGCTTGCCTCAATTGGGTTTGCAGCAGAAGGATTGCATCGAAATGAGCTGGGTTGAATCAATTCTGTTTTGGGCTAGCTACCCTATTGGGACTTCCCTTGATGTTTTGCTCAGTAGGGTTCCAAAGGGACTAACATTCCTGAAGCGGAAATCAGATTATGTGCAGGAACCGATTTCAAAGACAGATTTGGAAGCTTTGTGGAAAGTGATGATAGAAATTGGTGATGGTGGAATGCTGTGGAATCCTTATGGAGGAAAAATGAGTGAGATTTCAGCCACTGAAACTCCATTTCCACATAGAGCAGGCAACATATTCAAGATCCAATATTCATTTAACTGGAAAGATGGAGAGCTTGAGAATACCAACAGGTACTTAAATTTGACTAGACAACTTCATGAGGCAATGACTCCTCATGTGTCCAAGAATCCAAGGGAGGCTTTTCTCAACTACCGGGATATCGACATTGGAACCAATACAGAGGGTGAATACAAGGAAGGAGAAGTGTATGGGATTAAGTACTTCAAGCACAATTTTGAAAGATTAGTTTGGATTAAAACAAAGGTAGATCCCGATAATTTCTTTAGAAATGAACAGAGCATTCCAACTCTTCCGCCCCAAGGCATACCAAGGAGATCTCCGGGCCCAAGCAGCCGATTTGGGCCCAGTCCGCCGTCTTCTCCATCAATCCGTCGTACGATCCTTGCCGCAGAGTTCCGTCCGATCCATTCCAACGACAAACGTCGTCAAGATCCACGTCCCCATGGCAGCGAGCGATCCACTCGCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009505 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0030312 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

298

Amino Acids

34.05

Weight (kDa)

9.3

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BBE PF08031 182 - 237 1.2e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013793)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30710 AT1G30720 AT1G30730 AT1G34575 AT2G34810
fragaria_vesca FvH4_3g11041
malus_domestica MD05G1267400.v1.1 MD05G1268700.v1.1
prunus_persica Prupe.4G098800_v2.0.a1
pyrus_communis pycom05g25040
rosa_chinensis RchiOBHm_Chr5g0017761
rosa_laevigata RLG00000032313
rosa_multiflora Rmu_sc0009555.1_g000004
rosa_roxburghii Rroxscaffold_1G00059720 Rroxscaffold_1G00059730
rosa_rugosa Rorug05G0038500
rosa_wichuraiana Rw5G011420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 17
AciI CCGC 4 cut(s) 253, 719, 776, 813
AclWI GGATC 6 cut(s) 421, 668, 799, 823, 851, 876
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 3 cut(s) 479, 626, 802
AfiI CCNNNNNNNGG 4 cut(s) 17, 122, 359, 725
AgsI TTSAA 5 cut(s) 164, 285, 424, 631, 644
AjiI CACGTC 1 cut(s) 863
AjnI CCWGG 2 cut(s) 10, 69
AluBI AGCT 5 cut(s) 111, 156, 186, 302, 460
AluI AGCT 5 cut(s) 111, 156, 186, 302, 460
AlwI GGATC 6 cut(s) 421, 668, 799, 823, 851, 876
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 3 cut(s) 48, 748, 767
ApaI GGGCCC 2 cut(s) 752, 771
ApeKI GCWGC 4 cut(s) 97, 130, 756, 873
ApoI RAATTY 1 cut(s) 484
Asp700I GAANNNNTTC 1 cut(s) 168
AspS9I GGNCC 4 cut(s) 748, 749, 767, 768
AsuC2I CCSGG 2 cut(s) 560, 747
AsuHPI GGTGA 3 cut(s) 44, 341, 602
AsuNHI GCTAGC 1 cut(s) 182
BaeGI GKGCMC 2 cut(s) 752, 771
BanII GRGCYC 2 cut(s) 752, 771
BbsI GAAGAC 1 cut(s) 774
BbvI GCAGC 4 cut(s) 84, 142, 768, 885
BccI CCATC 3 cut(s) 326, 446, 796
BceAI ACGGC 2 cut(s) 63, 763
BciT130I CCWGG 2 cut(s) 12, 71
BcnI CCSGG 2 cut(s) 560, 747
BfaI CTAG 3 cut(s) 183, 492, 895
BglII AGATCT 1 cut(s) 740
BisI GCNGC 5 cut(s) 98, 131, 757, 813, 874
BlsI GCNGC 5 cut(s) 99, 132, 758, 814, 875
BmcAI AGTACT 1 cut(s) 626
Bme1390I CCNGG 4 cut(s) 12, 71, 560, 747
BmgBI CACGTC 1 cut(s) 863
BmgT120I GGNCC 4 cut(s) 748, 749, 767, 768
BmiI GGNNCC 5 cut(s) 228, 276, 577, 750, 769
BmrFI CCNGG 4 cut(s) 12, 71, 560, 747
BmrI ACTGGG 1 cut(s) 765
BmsI GCATC 1 cut(s) 153
BmtI GCTAGC 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 846
BpiI GAAGAC 1 cut(s) 774
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 2 cut(s) 560, 747
BsaJI CCNNGG 6 cut(s) 11, 70, 536, 724, 734, 868
BsaXI ACNNNNNCTCC 2 cut(s) 376, 406
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 122, 359, 725
Bse1I ACTGG 2 cut(s) 449, 771
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 2 cut(s) 12, 71
BseDI CCNNGG 6 cut(s) 11, 70, 536, 724, 734, 868
BseLI CCNNNNNNNGG 4 cut(s) 17, 122, 359, 725
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 83, 232
BseNI ACTGG 2 cut(s) 449, 771
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 2 cut(s) 752, 771
BseXI GCAGC 4 cut(s) 84, 142, 768, 885
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 290
BshFI GGCC 3 cut(s) 50, 750, 769
BsiSI CCGG 2 cut(s) 559, 746
BsiWI CGTACG 1 cut(s) 800
BslFI GGGAC 3 cut(s) 211, 249, 849
BslI CCNNNNNNNGG 4 cut(s) 17, 122, 359, 725
BsmFI GGGAC 3 cut(s) 211, 249, 849
BsmI GAATGC 2 cut(s) 345, 705
BsnI GGCC 3 cut(s) 50, 750, 769
Bsp120I GGGCCC 2 cut(s) 748, 767
Bsp1286I GDGCHC 2 cut(s) 752, 771
Bsp143I GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
Bsp19I CCATGG 1 cut(s) 868
BspACI CCGC 4 cut(s) 253, 719, 776, 813
BspANI GGCC 3 cut(s) 50, 750, 769
BspCNI CTCAG 2 cut(s) 84, 231
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 5 cut(s) 228, 276, 577, 750, 769
BspOI GCTAGC 1 cut(s) 186
BspPI GGATC 6 cut(s) 421, 668, 799, 823, 851, 876
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 2 cut(s) 449, 771
BssECI CCNNGG 6 cut(s) 11, 70, 536, 724, 734, 868
BssMI GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
BssT1I CCWWGG 4 cut(s) 536, 724, 734, 868
Bst2UI CCWGG 2 cut(s) 12, 71
Bst6I CTCTTC 1 cut(s) 720
BstC8I GCNNGC 4 cut(s) 113, 184, 412, 878
BstDEI CTNAG 2 cut(s) 92, 218
BstDSI CCRYGG 1 cut(s) 868
BstENI CCTNNNNNAGG 1 cut(s) 357
BstKTI GATC 7 cut(s) 429, 676, 743, 807, 831, 859, 884
BstMBI GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
BstMWI GCNNNNNNNGC 3 cut(s) 94, 756, 775
BstNI CCWGG 2 cut(s) 12, 71
BstPAI GACNNNNGTC 1 cut(s) 846
BstSCI CCNGG 4 cut(s) 10, 69, 558, 745
BstSLI GKGCMC 2 cut(s) 752, 771
BstV1I GCAGC 4 cut(s) 84, 142, 768, 885
BstV2I GAAGAC 1 cut(s) 774
BstX2I RGATCY 4 cut(s) 426, 673, 740, 856
BstYI RGATCY 4 cut(s) 426, 673, 740, 856
BsuRI GGCC 3 cut(s) 50, 750, 769
BtgI CCRYGG 1 cut(s) 868
BtrI CACGTC 1 cut(s) 863
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 4 cut(s) 113, 184, 412, 878
CaiI CAGNNNCTG 1 cut(s) 386
CciI TCATGA 1 cut(s) 502
Cfr13I GGNCC 4 cut(s) 748, 749, 767, 768
Csp6I GTAC 3 cut(s) 478, 625, 801
CviAII CATG 3 cut(s) 503, 521, 869
CviQI GTAC 3 cut(s) 478, 625, 801
DdeI CTNAG 2 cut(s) 92, 218
DpnI GATC 7 cut(s) 428, 675, 742, 806, 830, 858, 883
DpnII GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
EciI GGCGGA 2 cut(s) 708, 765
Eco130I CCWWGG 4 cut(s) 536, 724, 734, 868
Eco24I GRGCYC 2 cut(s) 752, 771
Eco32I GATATC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 357
EcoRII CCWGG 2 cut(s) 10, 69
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 4 cut(s) 536, 724, 734, 868
EcoT38I GRGCYC 2 cut(s) 752, 771
ErhI CCWWGG 4 cut(s) 536, 724, 734, 868
FaeI CATG 3 cut(s) 506, 524, 872
FaiI YATR 9 cut(s) 267, 357, 405, 419, 504, 522, 615, 731, 870
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 3 cut(s) 211, 249, 849
FatI CATG 3 cut(s) 502, 520, 868
Fnu4HI GCNGC 5 cut(s) 98, 131, 757, 813, 874
FriOI GRGCYC 2 cut(s) 752, 771
Fsp4HI GCNGC 5 cut(s) 98, 131, 757, 813, 874
FspBI CTAG 3 cut(s) 183, 492, 895
GluI GCNGC 5 cut(s) 98, 131, 757, 813, 874
GsaI CCCAGC 1 cut(s) 160
HaeIII GGCC 3 cut(s) 50, 750, 769
HapII CCGG 2 cut(s) 559, 746
Hin1II CATG 3 cut(s) 506, 524, 872
HindIII AAGCTT 2 cut(s) 109, 300
HinfI GANTC 6 cut(s) 77, 103, 164, 349, 514, 532
HpaII CCGG 2 cut(s) 559, 746
HphI GGTGA 3 cut(s) 44, 341, 602
Hpy188I TCNGA 3 cut(s) 93, 262, 828
Hpy188III TCNNGA 5 cut(s) 247, 424, 503, 677, 854
Hpy99I CGWCG 2 cut(s) 801, 852
HpyAV CCTTC 3 cut(s) 23, 130, 596
HpyCH4IV ACGT 2 cut(s) 847, 862
HpyCH4V TGCA 3 cut(s) 130, 144, 271
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 756, 775
HpyF3I CTNAG 2 cut(s) 92, 218
HpySE526I ACGT 2 cut(s) 847, 862
Hsp92II CATG 3 cut(s) 506, 524, 872
Kzo9I GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
LmnI GCTCC 1 cut(s) 896
Lsp1109I GCAGC 4 cut(s) 84, 142, 768, 885
LweI GCATC 1 cut(s) 153
MaeI CTAG 3 cut(s) 183, 492, 895
MaeII ACGT 2 cut(s) 847, 862
MaeIII GTNAC 1 cut(s) 6
MalI GATC 7 cut(s) 428, 675, 742, 806, 830, 858, 883
MboI GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
MboII GAAGA 2 cut(s) 707, 774
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 4 cut(s) 426, 673, 740, 856
MhlI GDGCHC 2 cut(s) 752, 771
MluCI AATT 6 cut(s) 119, 168, 324, 484, 637, 682
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 2 cut(s) 734, 861
MnlI CCTC 7 cut(s) 87, 126, 353, 499, 528, 535, 580
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 4 cut(s) 441, 482, 621, 660
MslI CAYNNNNRTG 1 cut(s) 149
MspI CCGG 2 cut(s) 559, 746
MspR9I CCNGG 4 cut(s) 12, 71, 560, 747
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 2 cut(s) 345, 705
MvaI CCWGG 2 cut(s) 12, 71
MwoI GCNNNNNNNGC 3 cut(s) 94, 756, 775
NciI CCSGG 2 cut(s) 560, 747
NcoI CCATGG 1 cut(s) 868
NdeII GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
NheI GCTAGC 1 cut(s) 182
NlaIII CATG 3 cut(s) 506, 524, 872
NlaIV GGNNCC 5 cut(s) 228, 276, 577, 750, 769
PagI TCATGA 1 cut(s) 502
PctI GAATGC 2 cut(s) 345, 705
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 5 cut(s) 77, 103, 164, 349, 532
Pfl23II CGTACG 1 cut(s) 800
PflMI CCANNNNNTGG 1 cut(s) 17
PkrI GCNGC 5 cut(s) 99, 132, 758, 814, 875
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PshAI GACNNNNGTC 1 cut(s) 846
Psp6I CCWGG 2 cut(s) 10, 69
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 2 cut(s) 10, 69
PspLI CGTACG 1 cut(s) 800
PspN4I GGNNCC 5 cut(s) 228, 276, 577, 750, 769
PspOMI GGGCCC 2 cut(s) 748, 767
PspPI GGNCC 4 cut(s) 748, 749, 767, 768
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 4 cut(s) 426, 673, 740, 856
RsaI GTAC 3 cut(s) 479, 626, 802
RsaNI GTAC 3 cut(s) 478, 625, 801
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 4 cut(s) 441, 482, 621, 660
SatI GCNGC 5 cut(s) 98, 131, 757, 813, 874
Sau3AI GATC 7 cut(s) 426, 673, 740, 804, 828, 856, 881
Sau96I GGNCC 4 cut(s) 748, 749, 767, 768
ScaI AGTACT 1 cut(s) 626
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 4 cut(s) 12, 71, 560, 747
SduI GDGCHC 2 cut(s) 752, 771
SfaNI GCATC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 6 cut(s) 119, 168, 324, 484, 637, 682
SsiI CCGC 4 cut(s) 253, 719, 776, 813
SspI AATATT 1 cut(s) 434
SspMI CTAG 3 cut(s) 183, 492, 895
StyD4I CCNGG 4 cut(s) 10, 69, 558, 745
StyI CCWWGG 4 cut(s) 536, 724, 734, 868
TaiI ACGT 2 cut(s) 850, 865
TaqI TCGA 2 cut(s) 147, 567
TasI AATT 6 cut(s) 119, 168, 324, 484, 637, 682
TatI WGTACW 1 cut(s) 624
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 5 cut(s) 77, 103, 164, 349, 532
Tru1I TTAA 4 cut(s) 441, 482, 621, 660
Tru9I TTAA 4 cut(s) 441, 482, 621, 660
TscAI CASTG 1 cut(s) 391
TseI GCWGC 4 cut(s) 97, 130, 756, 873
TspDTI ATGAA 3 cut(s) 426, 491, 711
TspGWI ACGGA 2 cut(s) 785, 812
TspRI CASTG 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 357
XapI RAATTY 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 3 cut(s) 183, 492, 895
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.