pycom05g25040

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013793
Physical Location & Seq
Forward (+)
26516806 .. 26518377
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25040.1

Sequence Viewer

Length: 1572 bp
ATGAAGGCCTTAGAATATGCACTACTATTATGTGCAGCATTTCCAATCTTTAGTTTGATTTCAGTGTCTTGTATCAATTCATCCTCAGTTGAATACAATTTTCTCCAATGCCTTTCAAATCATTCTCATTCCTCAAATCCAATCTCTGAGGCACTTTTCACCCCCAAAAGTGCCTCTTTTTCTTCTATTCTGCAGTCCTATGTGAGAAATCTCAGATTTATGGATCCCATAATCCCGAAACCTATGGTAATTATTGCAGCCAAGCACGAATCCCATGTTCAAGCCACAGTCATATGTGCCAAAACCCTTGGCTTGGAAATTAGAATTAGAAGTGGCGGACATGACTATGAAGGCGTCTCCTATGTATCCAGAGACGCAAAAGATTTCATCCTTCTTGACATGTTTAATCTTCGGTCCATCAACGTTGATTTGGCAGATGAAAGTGCATGGGTTCAATCTGGGGCTATACTTGGTGAAATTTTTTATGCAATTTTTAAGAAAAGCAAGTACCATGCCTTCCCTGCTGGGGTCTGTCCTACAATTGGTGCTGGTGGGCATTTCACAGGAGGTGGTTATGGTAACATGATGAGAAAATATGGGCTTTCCTCTGATAACATTGTTGATGCAAAAATAGTAAATGTAAATGGTACAATTCTTGATAGAAAATCAATGCGGGAAGATCTATTTTGGGCCATTAGAGGAGGTGGTGGATCAAGTTTTGGGGTCATTCTTGCTTGGAAAATCAAGCTGGTCCGGGTTCCTGCCATGGTAACGGTGTTTGATGTCAAAAAAACATTGGCAGAAGGTGCAACAGACATTCTTGTGAAATGGCAACAAGTTGCTGATAAGCTAGATAATGATCTCTTCATAAGAGCCGTTCATAAACCAGTCAATCGAACAATTGAGGTTTCTTTTGTTTCCTTGTTTTTGGGGAACTCCGATAGACTTTTGAAGCTGATGAGCCAAAGCCTTCCTCAATTGGGTTTGCAGAAAAAAGATTGCATAGAGATGAGTTGGATTCAATCGGTTTTGTTTTGGGCTGACTACAGAAATGGAACCACTCCTCCAGATGTTCTGCTCAACAGGTTCCCCAGCTCGGGAAAAGTGTTCTTGAAGCGCAAATCTGATTACGTGAAAGAACCAATTTCAAAGGCGGGTTTGGAAGCCTTGTGGAAGGTGATGATTGAGATTGGAGAAGTTATGATGCTGTGGAATCCTTATGGAGGAAAAATGAGTGAGATTCCTGAGACAGCTACTCCATTTCCTCATAGAGCAGGCAACCTATTCAAGATTCAGTATTCAGTTAACTGGAAGGAGGAAGGGGTTGAGGCCACCAAAAAGTACTTGCGTTTGATGAAAAAACTTTTTGAAGCAATGACTCCTCATGTGTCCAAGAATCCAAGGGAGGCTTTTTACAACTATCGTGACCTTGACGTTGGTACGAATTTAAAAGGTGTGTTGAATAAGAAGAGTACCCAAGATAACTCTAATGATCCCAAGAAGAAGAAAATAGAGCACACACTTAAAGAAAGCTCACAAAACAAACTAATTAGCAAAGCTTTTCTTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009505 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0030312 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

524

Amino Acids

58.56

Weight (kDa)

9.37

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 82 - 219 1.3e-20 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013793)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30710 AT1G30720 AT1G30730 AT1G34575 AT2G34810
fragaria_vesca FvH4_3g11041
malus_domestica MD05G1267400.v1.1 MD05G1268700.v1.1
prunus_persica Prupe.4G098800_v2.0.a1
pyrus_communis pycom05g25040
rosa_chinensis RchiOBHm_Chr5g0017761
rosa_laevigata RLG00000032313
rosa_multiflora Rmu_sc0009555.1_g000004
rosa_roxburghii Rroxscaffold_1G00059720 Rroxscaffold_1G00059730
rosa_rugosa Rorug05G0038500
rosa_wichuraiana Rw5G011420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 336, 673, 1154
AclI AACGTT 1 cut(s) 423
AclWI GGATC 4 cut(s) 218, 231, 718, 1487
AcsI RAATTY 2 cut(s) 477, 1444
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 5 cut(s) 509, 649, 1343, 1441, 1474
AfiI CCNNNNNNNGG 7 cut(s) 313, 526, 542, 980, 1096, 1097, 1223
AflIII ACRYGT 1 cut(s) 399
AloI GAACNNNNNNTCC 2 cut(s) 1048, 1080
AluBI AGCT 7 cut(s) 748, 850, 955, 1095, 1253, 1533, 1559
AluI AGCT 7 cut(s) 748, 850, 955, 1095, 1253, 1533, 1559
Alw21I GWGCWC 1 cut(s) 1518
Alw26I GTCTC 3 cut(s) 361, 366, 1241
AlwI GGATC 4 cut(s) 218, 231, 718, 1487
Ama87I CYCGRG 1 cut(s) 1096
AoxI GGCC 3 cut(s) 6, 690, 1329
ApeKI GCWGC 2 cut(s) 35, 257
ApoI RAATTY 2 cut(s) 477, 1444
AspLEI GCGC 1 cut(s) 1119
AspS9I GGNCC 3 cut(s) 414, 690, 751
AsuC2I CCSGG 1 cut(s) 755
AsuHPI GGTGA 3 cut(s) 151, 485, 1189
AvaI CYCGRG 1 cut(s) 1096
AvaII GGWCC 2 cut(s) 414, 751
BamHI GGATCC 1 cut(s) 223
BarI GAAGNNNNNNTAC 2 cut(s) 500, 532
Bbv12I GWGCWC 1 cut(s) 1518
BbvI GCAGC 2 cut(s) 47, 269
BccI CCATC 1 cut(s) 425
BceAI ACGGC 1 cut(s) 860
BciVI GTATCC 1 cut(s) 376
BcnI CCSGG 1 cut(s) 755
BcoDI GTCTC 3 cut(s) 361, 366, 1241
BfaI CTAG 1 cut(s) 851
BfmI CTRYAG 2 cut(s) 191, 1045
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 679
BisI GCNGC 2 cut(s) 36, 258
BlsI GCNGC 2 cut(s) 37, 259
BmcAI AGTACT 1 cut(s) 1343
Bme1390I CCNGG 1 cut(s) 755
Bme18I GGWCC 2 cut(s) 414, 751
BmeT110I CYCGRG 1 cut(s) 1096
BmgT120I GGNCC 3 cut(s) 414, 690, 751
BmiI GGNNCC 4 cut(s) 225, 759, 1057, 1088
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 2 cut(s) 613, 1194
BpmI CTGGAG 1 cut(s) 1050
BpuMI CCSGG 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 1132
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 307, 765, 1400
BsaXI ACNNNNNCTCC 4 cut(s) 1048, 1078, 1240, 1270
Bsc4I CCNNNNNNNGG 7 cut(s) 313, 526, 542, 980, 1096, 1097, 1223
Bse1I ACTGG 2 cut(s) 887, 1313
Bse3DI GCAATG 1 cut(s) 1380
Bse8I GATNNNNATC 1 cut(s) 858
BseDI CCNNGG 3 cut(s) 307, 765, 1400
BseGI GGATG 2 cut(s) 80, 387
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 7 cut(s) 313, 526, 542, 980, 1096, 1097, 1223
BseMI GCAATG 1 cut(s) 1380
BseMII CTCAG 4 cut(s) 99, 138, 226, 1236
BseNI ACTGG 2 cut(s) 887, 1313
BseRI GAGGAG 3 cut(s) 714, 1053, 1371
BseXI GCAGC 2 cut(s) 47, 269
BseYI CCCAGC 2 cut(s) 524, 1091
BsgI GTGCAG 1 cut(s) 54
BshFI GGCC 3 cut(s) 8, 692, 1331
BsiHKAI GWGCWC 1 cut(s) 1518
BsiHKCI CYCGRG 1 cut(s) 1096
BsiSI CCGG 1 cut(s) 754
BslI CCNNNNNNNGG 7 cut(s) 313, 526, 542, 980, 1096, 1097, 1223
BsmAI GTCTC 3 cut(s) 361, 366, 1241
BsmBI CGTCTC 2 cut(s) 361, 366
BsnI GGCC 3 cut(s) 8, 692, 1331
BsoBI CYCGRG 1 cut(s) 1096
Bsp1286I GDGCHC 1 cut(s) 1518
Bsp143I GATC 5 cut(s) 223, 679, 710, 859, 1492
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 3 cut(s) 336, 673, 1154
BspANI GGCC 3 cut(s) 8, 692, 1331
BspCNI CTCAG 4 cut(s) 98, 139, 225, 1237
BspLI GGNNCC 4 cut(s) 225, 759, 1057, 1088
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 4 cut(s) 218, 231, 718, 1487
BsrDI GCAATG 1 cut(s) 1380
BsrI ACTGG 2 cut(s) 887, 1313
BssECI CCNNGG 3 cut(s) 307, 765, 1400
BssMI GATC 5 cut(s) 223, 679, 710, 859, 1492
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 3 cut(s) 307, 765, 1400
Bst4CI ACNGT 2 cut(s) 289, 775
Bst6I CTCTTC 2 cut(s) 869, 1463
BstACI GRCGYC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 1132
BstC8I GCNNGC 1 cut(s) 1276
BstDEI CTNAG 5 cut(s) 10, 85, 147, 212, 1245
BstDSI CCRYGG 1 cut(s) 765
BstENI CCTNNNNNAGG 1 cut(s) 1221
BstF5I GGATG 2 cut(s) 80, 387
BstHHI GCGC 1 cut(s) 1119
BstKTI GATC 5 cut(s) 226, 682, 713, 862, 1495
BstMAI GTCTC 3 cut(s) 361, 366, 1241
BstMBI GATC 5 cut(s) 223, 679, 710, 859, 1492
BstMWI GCNNNNNNNGC 2 cut(s) 521, 806
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 2 cut(s) 191, 1045
BstV1I GCAGC 2 cut(s) 47, 269
BstX2I RGATCY 2 cut(s) 223, 679
BstYI RGATCY 2 cut(s) 223, 679
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 3 cut(s) 8, 692, 1331
BtgI CCRYGG 1 cut(s) 765
BtsCI GGATG 2 cut(s) 80, 387
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 1276
CfoI GCGC 1 cut(s) 1119
Cfr13I GGNCC 3 cut(s) 414, 690, 751
CseI GACGC 2 cut(s) 343, 383
Csp6I GTAC 5 cut(s) 508, 648, 1342, 1440, 1473
CviAII CATG 8 cut(s) 275, 341, 400, 447, 512, 583, 766, 1385
CviQI GTAC 5 cut(s) 508, 648, 1342, 1440, 1473
DdeI CTNAG 5 cut(s) 10, 85, 147, 212, 1245
DpnI GATC 5 cut(s) 225, 681, 712, 861, 1494
DpnII GATC 5 cut(s) 223, 679, 710, 859, 1492
DraI TTTAAA 1 cut(s) 1449
Eam1104I CTCTTC 2 cut(s) 869, 1463
EarI CTCTTC 2 cut(s) 869, 1463
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 3 cut(s) 307, 765, 1400
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 414, 751
Eco88I CYCGRG 1 cut(s) 1096
EcoNI CCTNNNNNAGG 1 cut(s) 1221
EcoT14I CCWWGG 3 cut(s) 307, 765, 1400
ErhI CCWWGG 3 cut(s) 307, 765, 1400
Esp3I CGTCTC 2 cut(s) 361, 366
FaeI CATG 8 cut(s) 278, 344, 403, 450, 515, 586, 769, 1388
FalI AAGNNNNNCTT 5 cut(s) 160, 192, 1393, 1425, 1548
FatI CATG 8 cut(s) 274, 340, 399, 446, 511, 582, 765, 1384
FauI CCCGC 2 cut(s) 666, 1147
FauNDI CATATG 1 cut(s) 293
Fnu4HI GCNGC 2 cut(s) 36, 258
FokI GGATG 2 cut(s) 67, 374
Fsp4HI GCNGC 2 cut(s) 36, 258
FspBI CTAG 1 cut(s) 851
GlaI GCGC 1 cut(s) 1118
GluI GCNGC 2 cut(s) 36, 258
GsaI CCCAGC 2 cut(s) 528, 1095
GsuI CTGGAG 1 cut(s) 1050
HaeIII GGCC 3 cut(s) 8, 692, 1331
HapII CCGG 1 cut(s) 754
HgaI GACGC 2 cut(s) 343, 383
HhaI GCGC 1 cut(s) 1119
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 8 cut(s) 278, 344, 403, 450, 515, 586, 769, 1388
Hin6I GCGC 1 cut(s) 1117
HinP1I GCGC 1 cut(s) 1117
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HindIII AAGCTT 1 cut(s) 1557
HinfI GANTC 7 cut(s) 269, 1018, 1213, 1240, 1291, 1378, 1396
HpaI GTTAAC 1 cut(s) 1306
HpaII CCGG 1 cut(s) 754
HphI GGTGA 3 cut(s) 151, 485, 1189
Hpy166II GTNNAC 1 cut(s) 1306
Hpy188I TCNGA 5 cut(s) 148, 215, 610, 940, 1126
Hpy8I GTNNAC 1 cut(s) 1306
HpyAV CCTTC 8 cut(s) 344, 401, 526, 797, 980, 1168, 1306, 1313
HpyCH4III ACNGT 2 cut(s) 289, 775
HpyCH4IV ACGT 3 cut(s) 423, 1131, 1434
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 806
HpyF3I CTNAG 5 cut(s) 10, 85, 147, 212, 1245
HpySE526I ACGT 3 cut(s) 423, 1131, 1434
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 8 cut(s) 278, 344, 403, 450, 515, 586, 769, 1388
HspAI GCGC 1 cut(s) 1117
KspAI GTTAAC 1 cut(s) 1306
Kzo9I GATC 5 cut(s) 223, 679, 710, 859, 1492
Lsp1109I GCAGC 2 cut(s) 47, 269
LweI GCATC 2 cut(s) 613, 1194
MaeI CTAG 1 cut(s) 851
MaeII ACGT 3 cut(s) 423, 1131, 1434
MaeIII GTNAC 3 cut(s) 578, 769, 1424
MalI GATC 5 cut(s) 225, 681, 712, 861, 1494
MboI GATC 5 cut(s) 223, 679, 710, 859, 1492
MboII GAAGA 7 cut(s) 174, 401, 689, 856, 1480, 1513, 1516
MfeI CAATTG 3 cut(s) 540, 900, 977
MflI RGATCY 2 cut(s) 223, 679
MhlI GDGCHC 1 cut(s) 1518
MlyI GAGTC 1 cut(s) 1372
MmeI TCCRAC 1 cut(s) 995
MseI TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
MslI CAYNNNNRTG 3 cut(s) 345, 821, 1007
MspI CCGG 1 cut(s) 754
MspR9I CCNGG 1 cut(s) 755
MunI CAATTG 3 cut(s) 540, 900, 977
MwoI GCNNNNNNNGC 2 cut(s) 521, 806
NciI CCSGG 1 cut(s) 755
NcoI CCATGG 1 cut(s) 765
NdeI CATATG 1 cut(s) 293
NdeII GATC 5 cut(s) 223, 679, 710, 859, 1492
NlaIII CATG 8 cut(s) 278, 344, 403, 450, 515, 586, 769, 1388
NlaIV GGNNCC 4 cut(s) 225, 759, 1057, 1088
NmuCI GTSAC 1 cut(s) 1424
NspI RCATGY 1 cut(s) 403
PceI AGGCCT 1 cut(s) 8
PciI ACATGT 1 cut(s) 399
PfeI GAWTC 6 cut(s) 269, 1018, 1213, 1240, 1291, 1396
PkrI GCNGC 2 cut(s) 37, 259
PleI GAGTC 1 cut(s) 1372
PpsI GAGTC 1 cut(s) 1372
Ppu21I YACGTR 1 cut(s) 1132
PscI ACATGT 1 cut(s) 399
Psp1406I AACGTT 1 cut(s) 423
PspFI CCCAGC 2 cut(s) 524, 1091
PspN4I GGNNCC 4 cut(s) 225, 759, 1057, 1088
PspPI GGNCC 3 cut(s) 414, 690, 751
PstI CTGCAG 1 cut(s) 195
PsuI RGATCY 2 cut(s) 223, 679
RsaI GTAC 5 cut(s) 509, 649, 1343, 1441, 1474
RsaNI GTAC 5 cut(s) 508, 648, 1342, 1440, 1473
RseI CAYNNNNRTG 3 cut(s) 345, 821, 1007
SaqAI TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
SatI GCNGC 2 cut(s) 36, 258
Sau3AI GATC 5 cut(s) 223, 679, 710, 859, 1492
Sau96I GGNCC 3 cut(s) 414, 690, 751
ScaI AGTACT 1 cut(s) 1343
SchI GAGTC 1 cut(s) 1372
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 1 cut(s) 1518
SfaNI GCATC 2 cut(s) 613, 1194
SfcI CTRYAG 2 cut(s) 191, 1045
SinI GGWCC 2 cut(s) 414, 751
SmiMI CAYNNNNRTG 3 cut(s) 345, 821, 1007
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 3 cut(s) 336, 673, 1154
SspMI CTAG 1 cut(s) 851
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 3 cut(s) 307, 765, 1400
TaaI ACNGT 2 cut(s) 289, 775
TaiI ACGT 3 cut(s) 426, 1134, 1437
TaqI TCGA 1 cut(s) 895
TaqII GACCGA 1 cut(s) 402
TatI WGTACW 1 cut(s) 1341
TfiI GAWTC 6 cut(s) 269, 1018, 1213, 1240, 1291, 1396
Tru1I TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
Tru9I TTAA 5 cut(s) 405, 495, 1305, 1448, 1524
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 1 cut(s) 1424
TseI GCWGC 2 cut(s) 35, 257
Tsp45I GTSAC 1 cut(s) 1424
TspDTI ATGAA 8 cut(s) 17, 69, 363, 376, 453, 856, 869, 1370
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 2 cut(s) 414, 751
XagI CCTNNNNNAGG 1 cut(s) 1221
XapI RAATTY 2 cut(s) 477, 1444
XceI RCATGY 1 cut(s) 403
XspI CTAG 1 cut(s) 851
ZrmI AGTACT 1 cut(s) 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.