Rorug05G0038500

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013793
Physical Location & Seq
Forward (+)
3090865 .. 3094603
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0038500.1

Sequence Viewer

Length: 2481 bp
ATGTTTTTGAATACTTTTCTTCTGTTCCTTCTCTTCCCTTTTTGCATTTCCGTAGACACCATAGGACCAAACCAGTATGTCAAGGATGGTGAGACCATAGTGTCTAAAGAAATGAAGTTTGAGCTATGCTTCTTTAGCCCTGGCAACTCTAGTTATCGGTATGTTGGAATTCGGTATGCTAAAATACCTGAAAAACCAGTGATATGGGTGGCAAACAGGGACAATCCGATAAATGATTCATCTGGTGTTCTCACAAACAGGTATGGAAACTTGGTTCTTTATGCTTATAAGGAAAACTTAACTATTTGGTCCACAAAGTCAGACCAAACTACTACTAGTACTTCAGCTCAAATTTTAGATACAGGAAACTTGATTTTGGTCCAGGGTAACGGTGAAGACTTTTTATGGCAAAGCTTTGATTATCCAACGGATACTATGATTCCAGGAATGAAACTTGGGTTGAATCGTGAGACCGGTCTGGATAGCTTCATAACATCCTGGAAGTCAGAAGACGACCCTGGAAATGGGGATTATTCATATAGGCTCAACCTGACTGGCTCCCCACAATTCATCCTGTATAATGGTTCAACTAGGTATTTTCGAACTGCTATGTGGCCATGGAGCCCAACACCGAGTACAACACCTTATGCATATGACTACACTTTTGTGAATAATCAAGATGTGACATATTTGATTGTTTCTTCTAATAAATCCTCTGAAATTTCAAGACTTGTGGTTAACTATTATGGCACACTTCAGCACCTAACATGGAATGATGTTGAGGGTCAATGGAAGGAAATGTGGGCTGCACCAAAGTTCAGGTGTGACCGATACGGACATTGTGGCGCCTTCAGTGGATGTAGTCTTGACAATGTTCAATCTGAGTGTTCTTGTTTGCCAGGGTATCAACCAAGTTCTTCAGAGAATTGGTCTGAGAAAATTTGGTCAGATGGGTGTGTGAGTAAGCAGGTAGAAGTATCTAATAAGTGTGGAAAAAGTTTCGTGAGGATGGAACATGTGAAGCTTCCAGATGCATCAATATCAGCTTTGCTGGACACAAATATGGTTGAAAGTGAGTGCAAGAGGGCATGCCAGAATAATTGTTCATGCGTTGCATATTCAAGTCTAGAAATTGATGGAGGTGGTGGTTGCATGGCATGGTATGGGGAATTGGTGGACACTTTAATACTGGCAGAGCAGGGACGAGATTTCTATGTTCGAGCAGATGCAACTGAGTCAGCTGAGAATGACAGAAAATCAAGAGGTTTCCTCAGAAGGAGGGGAATGCTTGCTGTTCTAATTATACCTGTTCTGCTGGCACTTTCTCTCATCATGTTGGCCTATTGGTGGTTTATCAAGAGGAAGACTGAAGCCGGTAGATTGAGAAGAACATGGGGGAGACAATACTCTCATTTTAGTACTTACAATGAGTCTATGGTAAAGAATAAAGTAGGGGAAACTAGTAGACACCCAGATTTGCAACTTTTCCATCTCAGCACAATAATAGCTGCAACAGATGACTTCTCTCTGGCTAATAAACTCGGAGAAGGTGGTTTTGGCTCTGTTTATAAGGGCCAGTTATCCAACCAACAGAAGATTGCTGTGAAAAGATTGTCCAAGAGTTCAAGACAAGGAGTTGAAGAATTTAAAAACGAAGTTACACTCATTGCAAAACTTCAACACAGGAATCTTGTGAAGCTATTAGGATGTTGTATTGAGGAAGAAGAGAAGTTGTTAATCTATGAATACTTGCCAAACAAAAGTTTGGACTTCTTTCTTTTTGATCAAAGTCAAAGGTCTGCCTTGGATTGGAGAAATCGATTTGGCATAATTACTGGTGTTGCTCGGGGAATTTTGTATCTCCACGAAGACTCAAGGTTGAGAATTGTCCACCGGGATCTGAAAGCCAGCAATGTCCTGCTTGATGCTGACATGAAGCCAAAAATTTCAGATTTCGGCTTGGCCAGAATATTTGATGGGGATCAACTGCAGGATCAGACAAAAAAAATTGTAGGAACATATGGTTATATGTCACCAGAGTATGCTGCGTATGGAAAATTTTCAACAAAATCAGATGTATTTAGCTTTGGGGTCATATTGTTGGAGATTATAAGTGGCAAGAAAAACAATGCTTCTTATCAGGAGGATCATTCCCTCAATTTGATAGGACATGTATGGGACCTATGGAGAGAACAGTGTGTCCTAGACATTGTAGATTTCCTACTGGAGTCATATAACATTGATGAAGTCCTGAGATGCCTTCAAATTGGGCTTTTATGCCTGCAAGAAGATACAACAGATAGGCCAACTATGTCCGAAGTTGTCCTCATGTTGAGTGGTGAAAGAGCTCTTGCATCCCCTCAAAGACCAGCATTCATCTTCAAAAACACTTGCAATTGCCACAATAGTTCCGGCTTATCAATAACAGGAGGATCTTATTCTGCTAATGATCTGACAGTCACTAAAGTCGTAGGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0005575 GO:0005618 GO:0005623 GO:0006950 GO:0008150 GO:0009505 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0030312 GO:0042221 GO:0044464 GO:0050896 GO:0071944 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

826

Amino Acids

93.43

Weight (kDa)

6.05

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 168 4.6e-34 D-mannose binding lectin
S_locus_glycop PF00954 216 - 305 1.2e-18 S-locus glycoprotein domain
PAN_2 PF08276 331 - 394 4.3e-16 PAN-like domain
PK_Tyr_Ser-Thr PF07714 510 - 775 4.2e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 510 - 775 2.5e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013793)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30710 AT1G30720 AT1G30730 AT1G34575 AT2G34810
fragaria_vesca FvH4_3g11041
malus_domestica MD05G1267400.v1.1 MD05G1268700.v1.1
prunus_persica Prupe.4G098800_v2.0.a1
pyrus_communis pycom05g25040
rosa_chinensis RchiOBHm_Chr5g0017761
rosa_laevigata RLG00000032313
rosa_multiflora Rmu_sc0009555.1_g000004
rosa_roxburghii Rroxscaffold_1G00059720 Rroxscaffold_1G00059730
rosa_rugosa Rorug05G0038500
rosa_wichuraiana Rw5G011420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 288, 1569, 2111
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 958
AccB1I GGYRCC 1 cut(s) 845
AccI GTMKAC 2 cut(s) 54, 1465
AclWI GGATC 5 cut(s) 1905, 1989, 2001, 2154, 2440
AcoI YGGCCR 2 cut(s) 614, 1962
AcsI RAATTY 8 cut(s) 168, 351, 720, 939, 1643, 1851, 1944, 2057
AcuI CTGAAG 5 cut(s) 327, 740, 835, 903, 1389
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 3 cut(s) 340, 637, 1420
AfiI CCNNNNNNNGG 3 cut(s) 524, 1347, 1809
AflIII ACRYGT 2 cut(s) 1015, 2170
AgeI ACCGGT 1 cut(s) 473
AhlI ACTAGT 2 cut(s) 335, 1460
AjnI CCWGG 6 cut(s) 139, 381, 442, 497, 517, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 1539, 1571
AleI CACNNNNGTG 1 cut(s) 665
AloI GAACNNNNNNTCC 4 cut(s) 258, 290, 2184, 2216
Alw21I GWGCWC 1 cut(s) 2350
Alw26I GTCTC 3 cut(s) 86, 464, 1393
AlwI GGATC 5 cut(s) 1905, 1989, 2001, 2154, 2440
Ama87I CYCGRG 1 cut(s) 1845
AoxI GGCC 5 cut(s) 614, 1338, 1573, 1962, 2303
ApeKI GCWGC 3 cut(s) 806, 1508, 2045
ApoI RAATTY 8 cut(s) 168, 351, 720, 939, 1643, 1851, 1944, 2057
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AsiGI ACCGGT 1 cut(s) 473
Asp700I GAANNNNTTC 1 cut(s) 2059
AspLEI GCGC 1 cut(s) 848
AspS9I GGNCC 5 cut(s) 65, 309, 379, 1573, 2179
AsuC2I CCSGG 1 cut(s) 1895
AsuHPI GGTGA 4 cut(s) 101, 404, 2025, 2351
AsuII TTCGAA 1 cut(s) 601
AvaI CYCGRG 1 cut(s) 1845
AvaII GGWCC 4 cut(s) 65, 309, 379, 2179
BalI TGGCCA 2 cut(s) 616, 1964
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 2 cut(s) 626, 2350
BbsI GAAGAC 4 cut(s) 402, 516, 1370, 1875
Bbv12I GWGCWC 1 cut(s) 2350
BbvI GCAGC 3 cut(s) 793, 1495, 2032
BccI CCATC 6 cut(s) 80, 944, 1003, 1130, 1497, 1970
BciT130I CCWGG 6 cut(s) 141, 383, 444, 499, 519, 900
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 1 cut(s) 1783
BcnI CCSGG 1 cut(s) 1895
BcoDI GTCTC 3 cut(s) 86, 464, 1393
BcuI ACTAGT 2 cut(s) 335, 1460
BfaI CTAG 6 cut(s) 150, 336, 591, 1127, 1461, 2204
BfmI CTRYAG 1 cut(s) 1988
BfoI RGCGCY 1 cut(s) 849
BfuAI ACCTGC 1 cut(s) 958
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 3 cut(s) 807, 1509, 2046
BlsI GCNGC 3 cut(s) 808, 1510, 2047
BmcAI AGTACT 2 cut(s) 340, 1420
Bme1390I CCNGG 7 cut(s) 141, 383, 444, 499, 519, 900, 1895
Bme18I GGWCC 4 cut(s) 65, 309, 379, 2179
BmeT110I CYCGRG 1 cut(s) 1845
BmgT120I GGNCC 5 cut(s) 65, 309, 379, 1573, 2179
BmiI GGNNCC 4 cut(s) 559, 623, 847, 2180
BmrFI CCNGG 7 cut(s) 141, 383, 444, 499, 519, 900, 1895
BmsI GCATC 6 cut(s) 1021, 1043, 1216, 1915, 2246, 2363
BpiI GAAGAC 4 cut(s) 402, 516, 1370, 1875
BplI GAGNNNNNCTC 2 cut(s) 1254, 1286
BpmI CTGGAG 1 cut(s) 2246
Bpu14I TTCGAA 1 cut(s) 601
BpuEI CTTGAG 1 cut(s) 1858
BpuMI CCSGG 1 cut(s) 1895
Bsa29I ATCGAT 1 cut(s) 1819
BsaBI GATNNNNATC 1 cut(s) 1980
BsaHI GRCGYC 1 cut(s) 846
BsaI GGTCTC 2 cut(s) 86, 464
BsaJI CCNNGG 6 cut(s) 139, 382, 517, 617, 899, 1803
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 1347, 1809
Bse118I RCCGGY 2 cut(s) 473, 1373
Bse1I ACTGG 7 cut(s) 73, 197, 559, 1194, 1576, 1840, 2229
Bse3DI GCAATG 2 cut(s) 1665, 1918
Bse8I GATNNNNATC 1 cut(s) 1980
BseBI CCWGG 6 cut(s) 141, 383, 444, 499, 519, 900
BseCI ATCGAT 1 cut(s) 1819
BseDI CCNNGG 6 cut(s) 139, 382, 517, 617, 899, 1803
BseGI GGATG 7 cut(s) 91, 494, 570, 863, 1014, 1712, 2354
BseJI GATNNNNATC 1 cut(s) 1980
BseLI CCNNNNNNNGG 3 cut(s) 524, 1347, 1809
BseMI GCAATG 2 cut(s) 1665, 1918
BseMII CTCAG 7 cut(s) 873, 924, 1224, 1233, 1285, 1507, 2243
BseNI ACTGG 7 cut(s) 73, 197, 559, 1194, 1576, 1840, 2229
BseXI GCAGC 3 cut(s) 793, 1495, 2032
BsgI GTGCAG 1 cut(s) 792
BshFI GGCC 5 cut(s) 616, 1340, 1575, 1964, 2305
BshNI GGYRCC 1 cut(s) 845
BshTI ACCGGT 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 1819
BsiHKAI GWGCWC 1 cut(s) 2350
BsiHKCI CYCGRG 1 cut(s) 1845
BsiSI CCGG 4 cut(s) 474, 1374, 1894, 2412
BslFI GGGAC 3 cut(s) 233, 1215, 2192
BslI CCNNNNNNNGG 3 cut(s) 524, 1347, 1809
BsmAI GTCTC 3 cut(s) 86, 464, 1393
BsmFI GGGAC 3 cut(s) 233, 1215, 2192
BsmI GAATGC 2 cut(s) 1290, 2372
BsnI GGCC 5 cut(s) 616, 1340, 1575, 1964, 2305
Bso31I GGTCTC 2 cut(s) 86, 464
BsoBI CYCGRG 1 cut(s) 1845
Bsp119I TTCGAA 1 cut(s) 601
Bsp1286I GDGCHC 2 cut(s) 626, 2350
Bsp143I GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
Bsp19I CCATGG 1 cut(s) 617
BspANI GGCC 5 cut(s) 616, 1340, 1575, 1964, 2305
BspCNI CTCAG 7 cut(s) 874, 925, 1225, 1234, 1284, 1506, 2244
BspDI ATCGAT 1 cut(s) 1819
BspLI GGNNCC 4 cut(s) 559, 623, 847, 2180
BspMAI CTGCAG 1 cut(s) 1992
BspMI ACCTGC 1 cut(s) 958
BspPI GGATC 5 cut(s) 1905, 1989, 2001, 2154, 2440
BspT104I TTCGAA 1 cut(s) 601
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 2 cut(s) 86, 464
BsrDI GCAATG 2 cut(s) 1665, 1918
BsrFI RCCGGY 2 cut(s) 473, 1373
BsrI ACTGG 7 cut(s) 73, 197, 559, 1194, 1576, 1840, 2229
BssAI RCCGGY 2 cut(s) 473, 1373
BssECI CCNNGG 6 cut(s) 139, 382, 517, 617, 899, 1803
BssMI GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
BssNI GRCGYC 1 cut(s) 846
BssT1I CCWWGG 2 cut(s) 617, 1803
Bst2UI CCWGG 6 cut(s) 141, 383, 444, 499, 519, 900
Bst4CI ACNGT 3 cut(s) 392, 2196, 2458
Bst6I CTCTTC 2 cut(s) 38, 1719
BstACI GRCGYC 1 cut(s) 846
BstBI TTCGAA 1 cut(s) 601
BstC8I GCNNGC 5 cut(s) 1090, 1290, 1317, 1909, 2282
BstDEI CTNAG 7 cut(s) 882, 933, 1233, 1242, 1271, 1493, 2252
BstDSI CCRYGG 1 cut(s) 617
BstF5I GGATG 7 cut(s) 91, 494, 570, 863, 1014, 1712, 2354
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 1 cut(s) 848
BstKTI GATC 7 cut(s) 1786, 1900, 1984, 1996, 2149, 2435, 2452
BstMAI GTCTC 3 cut(s) 86, 464, 1393
BstMBI GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 6 cut(s) 141, 383, 444, 499, 519, 900
BstNSI RCATGY 3 cut(s) 1019, 1092, 2174
BstSCI CCNGG 7 cut(s) 139, 381, 442, 497, 517, 898, 1893
BstSFI CTRYAG 1 cut(s) 1988
BstV1I GCAGC 3 cut(s) 793, 1495, 2032
BstV2I GAAGAC 4 cut(s) 402, 516, 1370, 1875
BstX2I RGATCY 2 cut(s) 1897, 2432
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 2 cut(s) 1897, 2432
Bsu15I ATCGAT 1 cut(s) 1819
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 5 cut(s) 616, 1340, 1575, 1964, 2305
BsuTUI ATCGAT 1 cut(s) 1819
BtgI CCRYGG 1 cut(s) 617
BtsCI GGATG 7 cut(s) 91, 494, 570, 863, 1014, 1712, 2354
BtsIMutI CAGTG 3 cut(s) 204, 859, 2201
BveI ACCTGC 1 cut(s) 958
Cac8I GCNNGC 5 cut(s) 1090, 1290, 1317, 1909, 2282
CfoI GCGC 1 cut(s) 848
Cfr10I RCCGGY 2 cut(s) 473, 1373
Cfr13I GGNCC 5 cut(s) 65, 309, 379, 1573, 2179
ClaI ATCGAT 1 cut(s) 1819
Csp6I GTAC 3 cut(s) 339, 636, 1419
CspAI ACCGGT 1 cut(s) 473
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviQI GTAC 3 cut(s) 339, 636, 1419
DdeI CTNAG 7 cut(s) 882, 933, 1233, 1242, 1271, 1493, 2252
DinI GGCGCC 1 cut(s) 847
DpnI GATC 7 cut(s) 1785, 1899, 1983, 1995, 2148, 2434, 2451
DpnII GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
DraI TTTAAA 1 cut(s) 1648
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 2 cut(s) 614, 1962
Eam1104I CTCTTC 2 cut(s) 38, 1719
EarI CTCTTC 2 cut(s) 38, 1719
Ecl136II GAGCTC 1 cut(s) 2348
Eco130I CCWWGG 2 cut(s) 617, 1803
Eco24I GRGCYC 2 cut(s) 626, 2350
Eco31I GGTCTC 2 cut(s) 86, 464
Eco47I GGWCC 4 cut(s) 65, 309, 379, 2179
Eco53kI GAGCTC 1 cut(s) 2348
Eco57I CTGAAG 5 cut(s) 327, 740, 835, 903, 1389
Eco88I CYCGRG 1 cut(s) 1845
EcoICRI GAGCTC 1 cut(s) 2348
EcoO109I RGGNCCY 1 cut(s) 2179
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 6 cut(s) 139, 381, 442, 497, 517, 898
EcoT14I CCWWGG 2 cut(s) 617, 1803
EcoT22I ATGCAT 2 cut(s) 652, 1036
EcoT38I GRGCYC 2 cut(s) 626, 2350
EgeI GGCGCC 1 cut(s) 847
EheI GGCGCC 1 cut(s) 847
ErhI CCWWGG 2 cut(s) 617, 1803
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 3 cut(s) 233, 1215, 2192
FauNDI CATATG 2 cut(s) 652, 2020
FbaI TGATCA 1 cut(s) 1783
FblI GTMKAC 2 cut(s) 54, 1465
Fnu4HI GCNGC 3 cut(s) 807, 1509, 2046
FokI GGATG 7 cut(s) 98, 481, 557, 870, 1021, 1719, 2341
FriOI GRGCYC 2 cut(s) 626, 2350
Fsp4HI GCNGC 3 cut(s) 807, 1509, 2046
FspBI CTAG 6 cut(s) 150, 336, 591, 1127, 1461, 2204
GlaI GCGC 1 cut(s) 847
GluI GCNGC 3 cut(s) 807, 1509, 2046
GsuI CTGGAG 1 cut(s) 2246
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 5 cut(s) 616, 1340, 1575, 1964, 2305
HapII CCGG 4 cut(s) 474, 1374, 1894, 2412
HhaI GCGC 1 cut(s) 848
Hin1I GRCGYC 1 cut(s) 846
Hin6I GCGC 1 cut(s) 846
HinP1I GCGC 1 cut(s) 846
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 412, 1022
HinfI GANTC 8 cut(s) 236, 439, 463, 1235, 1430, 1687, 1871, 2228
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 4 cut(s) 474, 1374, 1894, 2412
HphI GGTGA 4 cut(s) 101, 404, 2025, 2351
Hpy166II GTNNAC 6 cut(s) 55, 312, 739, 1177, 1466, 1891
Hpy8I GTNNAC 6 cut(s) 55, 312, 739, 1177, 1466, 1891
HpyAV CCTTC 6 cut(s) 38, 787, 859, 1269, 1541, 2270
HpyCH4III ACNGT 3 cut(s) 392, 2196, 2458
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 7 cut(s) 882, 933, 1233, 1242, 1271, 1493, 2252
Hsp92I GRCGYC 1 cut(s) 846
HspAI GCGC 1 cut(s) 846
KasI GGCGCC 1 cut(s) 845
Ksp22I TGATCA 1 cut(s) 1783
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
LmnI GCTCC 2 cut(s) 563, 621
Lsp1109I GCAGC 3 cut(s) 793, 1495, 2032
LweI GCATC 6 cut(s) 1021, 1043, 1216, 1915, 2246, 2363
MaeI CTAG 6 cut(s) 150, 336, 591, 1127, 1461, 2204
MaeIII GTNAC 6 cut(s) 386, 682, 824, 1657, 2031, 2458
MalI GATC 7 cut(s) 1785, 1899, 1983, 1995, 2148, 2434, 2451
MboI GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
MfeI CAATTG 1 cut(s) 2395
MflI RGATCY 2 cut(s) 1897, 2432
MhlI GDGCHC 2 cut(s) 626, 2350
MlsI TGGCCA 2 cut(s) 616, 1964
MluNI TGGCCA 2 cut(s) 616, 1964
Mly113I GGCGCC 1 cut(s) 846
MlyI GAGTC 4 cut(s) 1244, 1439, 1865, 2237
MmeI TCCRAC 4 cut(s) 145, 449, 1608, 2082
Mox20I TGGCCA 2 cut(s) 616, 1964
Mph1103I ATGCAT 2 cut(s) 652, 1036
MroXI GAANNNNTTC 1 cut(s) 2059
MscI TGGCCA 2 cut(s) 616, 1964
MseI TTAA 5 cut(s) 299, 738, 1184, 1647, 1736
MslI CAYNNNNRTG 2 cut(s) 665, 1061
Msp20I TGGCCA 2 cut(s) 616, 1964
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 4 cut(s) 474, 1374, 1894, 2412
MspR9I CCNGG 7 cut(s) 141, 383, 444, 499, 519, 900, 1895
MunI CAATTG 1 cut(s) 2395
Mva1269I GAATGC 2 cut(s) 1290, 2372
MvaI CCWGG 6 cut(s) 141, 383, 444, 499, 519, 900
MwoI GCNNNNNNNGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 846
NciI CCSGG 1 cut(s) 1895
NcoI CCATGG 1 cut(s) 617
NdeI CATATG 2 cut(s) 652, 2020
NdeII GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
NlaIV GGNNCC 4 cut(s) 559, 623, 847, 2180
NmuCI GTSAC 4 cut(s) 682, 824, 2031, 2458
NsiI ATGCAT 2 cut(s) 652, 1036
NspI RCATGY 3 cut(s) 1019, 1092, 2174
NspV TTCGAA 1 cut(s) 601
OliI CACNNNNGTG 1 cut(s) 665
PaeI GCATGC 1 cut(s) 1092
PciI ACATGT 2 cut(s) 1015, 2170
PctI GAATGC 2 cut(s) 1290, 2372
PdmI GAANNNNTTC 1 cut(s) 2059
PfeI GAWTC 4 cut(s) 236, 439, 463, 1687
PfoI TCCNGGA 2 cut(s) 442, 497
PinAI ACCGGT 1 cut(s) 473
PkrI GCNGC 3 cut(s) 808, 1510, 2047
PleI GAGTC 4 cut(s) 1243, 1438, 1865, 2236
PluTI GGCGCC 1 cut(s) 849
PpsI GAGTC 4 cut(s) 1243, 1438, 1865, 2236
PpuMI RGGWCCY 1 cut(s) 2179
PscI ACATGT 2 cut(s) 1015, 2170
PsiI TTATAA 3 cut(s) 288, 1569, 2111
Psp124BI GAGCTC 1 cut(s) 2350
Psp5II RGGWCCY 1 cut(s) 2179
Psp6I CCWGG 6 cut(s) 139, 381, 442, 497, 517, 898
PspGI CCWGG 6 cut(s) 139, 381, 442, 497, 517, 898
PspN4I GGNNCC 4 cut(s) 559, 623, 847, 2180
PspPI GGNCC 5 cut(s) 65, 309, 379, 1573, 2179
PspPPI RGGWCCY 1 cut(s) 2179
PstI CTGCAG 1 cut(s) 1992
PsuI RGATCY 2 cut(s) 1897, 2432
PvuII CAGCTG 1 cut(s) 1241
RsaI GTAC 3 cut(s) 340, 637, 1420
RsaNI GTAC 3 cut(s) 339, 636, 1419
RseI CAYNNNNRTG 2 cut(s) 665, 1061
SacI GAGCTC 1 cut(s) 2350
SaqAI TTAA 5 cut(s) 299, 738, 1184, 1647, 1736
SatI GCNGC 3 cut(s) 807, 1509, 2046
Sau3AI GATC 7 cut(s) 1783, 1897, 1981, 1993, 2146, 2432, 2449
Sau96I GGNCC 5 cut(s) 65, 309, 379, 1573, 2179
ScaI AGTACT 2 cut(s) 340, 1420
SchI GAGTC 4 cut(s) 1244, 1439, 1865, 2237
ScrFI CCNGG 7 cut(s) 141, 383, 444, 499, 519, 900, 1895
SduI GDGCHC 2 cut(s) 626, 2350
SfaNI GCATC 6 cut(s) 1021, 1043, 1216, 1915, 2246, 2363
SfcI CTRYAG 1 cut(s) 1988
SfoI GGCGCC 1 cut(s) 847
SfuI TTCGAA 1 cut(s) 601
SinI GGWCC 4 cut(s) 65, 309, 379, 2179
SmiMI CAYNNNNRTG 2 cut(s) 665, 1061
SmlI CTYRAG 1 cut(s) 1873
SmoI CTYRAG 1 cut(s) 1873
SpeI ACTAGT 2 cut(s) 335, 1460
SphI GCATGC 1 cut(s) 1092
SspDI GGCGCC 1 cut(s) 845
SspI AATATT 1 cut(s) 1971
SspMI CTAG 6 cut(s) 150, 336, 591, 1127, 1461, 2204
SstI GAGCTC 1 cut(s) 2350
StyD4I CCNGG 7 cut(s) 139, 381, 442, 497, 517, 898, 1893
StyI CCWWGG 2 cut(s) 617, 1803
TaaI ACNGT 3 cut(s) 392, 2196, 2458
TaqI TCGA 3 cut(s) 601, 1219, 1819
TaqII GACCGA 1 cut(s) 843
TatI WGTACW 3 cut(s) 338, 635, 1418
TfiI GAWTC 4 cut(s) 236, 439, 463, 1687
Tru1I TTAA 5 cut(s) 299, 738, 1184, 1647, 1736
Tru9I TTAA 5 cut(s) 299, 738, 1184, 1647, 1736
TscAI CASTG 3 cut(s) 204, 859, 2201
TseFI GTSAC 4 cut(s) 682, 824, 2031, 2458
TseI GCWGC 3 cut(s) 806, 1508, 2045
Tsp45I GTSAC 4 cut(s) 682, 824, 2031, 2458
TspGWI ACGGA 3 cut(s) 40, 443, 849
TspRI CASTG 3 cut(s) 204, 859, 2201
VpaK11BI GGWCC 4 cut(s) 65, 309, 379, 2179
XapI RAATTY 8 cut(s) 168, 351, 720, 939, 1643, 1851, 1944, 2057
XbaI TCTAGA 1 cut(s) 1126
XceI RCATGY 3 cut(s) 1019, 1092, 2174
XmiI GTMKAC 2 cut(s) 54, 1465
XmnI GAANNNNTTC 1 cut(s) 2059
XspI CTAG 6 cut(s) 150, 336, 591, 1127, 1461, 2204
ZrmI AGTACT 2 cut(s) 340, 1420
Zsp2I ATGCAT 2 cut(s) 652, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.