MD05G1272900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016687
Physical Location & Seq
Forward (+)
40799931 .. 40800713
783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1272900.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGCGTTTTTCATCCAAGTTGGTGTAGCAGCGATCGCGGTGTTTCTGATGGCGGTGGCGATGGAATCTGACGGGCGGGAGCTGCGTCCGTCTGATCACGGCCTCGTGTACCAGAACCCGGCGCCGGCCAGCGCGAAGTCGCCGGCGGATGAGAAGTCGTTCTTCGGCGGGGGAGGAGGAGAGAATTCTCCGGCGGCGGGGGACGCAGCACTGCCGAAGCCAAAGGCAATGAACTCGAGCGACGCCGACGCGTCGTGGTGGAAGGGCGTGGTGGCACGTGACGGCGGGAGGCGTAGAGATCACGTGGAGGACGTGTTGCTGGTGGCGAGCTTGGTTTGTGGGATTGCGGGTGTCGCTCTGTTCGTGGCCTCCGCTTTTGTTTACGTCTTAAGCATTTTGATTAAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

14.09

Weight (kDa)

5.28

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016687)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30515 AT4G21740
fragaria_vesca FvH4_3g10110 FvH4_3g10110
malus_domestica MD05G1272900.v1.1 MD10G1253000.v1.1
prunus_persica Prupe.4G090500_v2.0.a1
pyrus_communis pycom10g20870
rosa_chinensis RchiOBHm_Chr5g0016051
rosa_laevigata RLG00000032237
rosa_multiflora Rmu_sc0000299.1_g000015
rosa_roxburghii Rroxscaffold_1G00060600
rosa_rugosa Rorug05G0030900
rosa_wichuraiana Rw5G010770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AccII CGCG 3 cut(s) 38, 134, 251
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 184
AcvI CACGTG 2 cut(s) 278, 304
AcyI GRCGYC 2 cut(s) 122, 243
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 118, 124, 197
AflII CTTAAG 1 cut(s) 388
AflIII ACRYGT 2 cut(s) 249, 312
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 2 cut(s) 82, 330
AluI AGCT 2 cut(s) 82, 330
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 3 cut(s) 100, 126, 366
ApeKI GCWGC 3 cut(s) 29, 82, 206
ApoI RAATTY 1 cut(s) 184
AsiSI GCGATCGC 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 2 cut(s) 124, 134
AsuC2I CCSGG 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 235
BanI GGYRCC 1 cut(s) 121
BauI CACGAG 1 cut(s) 104
BbrPI CACGTG 2 cut(s) 278, 304
BbvI GCAGC 3 cut(s) 41, 69, 218
BccI CCATC 2 cut(s) 43, 55
BceAI ACGGC 2 cut(s) 115, 298
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 388
BisI GCNGC 4 cut(s) 30, 83, 195, 207
BlsI GCNGC 4 cut(s) 31, 84, 196, 208
Bme1390I CCNGG 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 119
BsaAI YACGTR 2 cut(s) 278, 304
BsaHI GRCGYC 2 cut(s) 122, 243
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 124, 197
Bse118I RCCGGY 2 cut(s) 124, 142
Bse3DI GCAATG 1 cut(s) 234
BseGI GGATG 2 cut(s) 12, 154
BseLI CCNNNNNNNGG 3 cut(s) 118, 124, 197
BseMI GCAATG 1 cut(s) 234
BseRI GAGGAG 2 cut(s) 189, 192
BseXI GCAGC 3 cut(s) 41, 69, 218
Bsh1236I CGCG 3 cut(s) 38, 134, 251
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 3 cut(s) 102, 128, 368
BshNI GGYRCC 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 235
BsiSI CCGG 4 cut(s) 119, 125, 143, 191
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 118, 124, 197
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 3 cut(s) 102, 128, 368
BsoBI CYCGRG 1 cut(s) 235
Bsp143I GATC 3 cut(s) 33, 94, 298
BspANI GGCC 3 cut(s) 102, 128, 368
BspFNI CGCG 3 cut(s) 38, 134, 251
BspLI GGNNCC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 2 cut(s) 124, 142
BssAI RCCGGY 2 cut(s) 124, 142
BssMI GATC 3 cut(s) 33, 94, 298
BssNI GRCGYC 2 cut(s) 122, 243
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
BstACI GRCGYC 2 cut(s) 122, 243
BstAFI CTTAAG 1 cut(s) 388
BstBAI YACGTR 2 cut(s) 278, 304
BstC8I GCNNGC 4 cut(s) 126, 130, 144, 328
BstF5I GGATG 2 cut(s) 12, 154
BstFNI CGCG 3 cut(s) 38, 134, 251
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 134
BstKTI GATC 3 cut(s) 36, 97, 301
BstMBI GATC 3 cut(s) 33, 94, 298
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 4 cut(s) 35, 82, 203, 353
BstSCI CCNGG 1 cut(s) 117
BstUI CGCG 3 cut(s) 38, 134, 251
BstV1I GCAGC 3 cut(s) 41, 69, 218
BsuRI GGCC 3 cut(s) 102, 128, 368
BtgZI GCGATG 1 cut(s) 74
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 12, 154
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 1 cut(s) 209
Cac8I GCNNGC 4 cut(s) 126, 130, 144, 328
CfoI GCGC 2 cut(s) 124, 134
Cfr10I RCCGGY 2 cut(s) 124, 142
CseI GACGC 5 cut(s) 74, 212, 240, 251, 257
Csp6I GTAC 1 cut(s) 109
CviJI RGCY 6 cut(s) 82, 102, 128, 220, 330, 368
CviKI_1 RGCY 6 cut(s) 82, 102, 128, 220, 330, 368
CviQI GTAC 1 cut(s) 109
DinI GGCGCC 1 cut(s) 123
DpnI GATC 3 cut(s) 35, 96, 300
DpnII GATC 3 cut(s) 33, 94, 298
EaeI YGGCCR 1 cut(s) 126
EciI GGCGGA 1 cut(s) 161
Eco72I CACGTG 2 cut(s) 278, 304
Eco88I CYCGRG 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 184
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 215
FauI CCCGC 5 cut(s) 69, 161, 190, 278, 340
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 4 cut(s) 30, 83, 195, 207
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 4 cut(s) 30, 83, 195, 207
GlaI GCGC 2 cut(s) 123, 133
GluI GCNGC 4 cut(s) 30, 83, 195, 207
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 3 cut(s) 102, 128, 368
HapII CCGG 4 cut(s) 119, 125, 143, 191
HgaI GACGC 5 cut(s) 74, 212, 240, 251, 257
HhaI GCGC 2 cut(s) 124, 134
Hin1I GRCGYC 2 cut(s) 122, 243
Hin6I GCGC 2 cut(s) 122, 132
HinP1I GCGC 2 cut(s) 122, 132
HinfI GANTC 1 cut(s) 65
HpaII CCGG 4 cut(s) 119, 125, 143, 191
Hpy166II GTNNAC 2 cut(s) 109, 382
Hpy188I TCNGA 3 cut(s) 48, 70, 94
Hpy8I GTNNAC 2 cut(s) 109, 382
Hpy99I CGWCG 3 cut(s) 245, 251, 256
HpyAV CCTTC 1 cut(s) 256
HpyCH4IV ACGT 4 cut(s) 277, 303, 312, 384
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 82, 203, 353
HpySE526I ACGT 4 cut(s) 277, 303, 312, 384
Hsp92I GRCGYC 2 cut(s) 122, 243
HspAI GCGC 2 cut(s) 122, 132
KasI GGCGCC 1 cut(s) 121
KroI GCCGGC 2 cut(s) 124, 142
KroNI GCCGGC 2 cut(s) 126, 144
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 3 cut(s) 33, 94, 298
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 125, 132, 138, 142, 156, 204, 305
Lsp1109I GCAGC 3 cut(s) 41, 69, 218
MaeII ACGT 4 cut(s) 277, 303, 312, 384
MaeIII GTNAC 1 cut(s) 278
MalI GATC 3 cut(s) 35, 96, 300
MboI GATC 3 cut(s) 33, 94, 298
MboII GAAGA 1 cut(s) 154
MluCI AATT 1 cut(s) 184
MluI ACGCGT 1 cut(s) 249
Mly113I GGCGCC 1 cut(s) 122
MnlI CCTC 6 cut(s) 113, 167, 170, 282, 301, 379
MreI CGCCGGCG 1 cut(s) 142
MroNI GCCGGC 2 cut(s) 124, 142
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 2 cut(s) 389, 402
MspCI CTTAAG 1 cut(s) 388
MspI CCGG 4 cut(s) 119, 125, 143, 191
MspR9I CCNGG 1 cut(s) 119
MvnI CGCG 3 cut(s) 38, 134, 251
MwoI GCNNNNNNNGC 4 cut(s) 35, 82, 203, 353
NaeI GCCGGC 2 cut(s) 126, 144
NarI GGCGCC 1 cut(s) 122
NciI CCSGG 1 cut(s) 119
NdeII GATC 3 cut(s) 33, 94, 298
NgoMIV GCCGGC 2 cut(s) 124, 142
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 278
PaeR7I CTCGAG 1 cut(s) 235
PcsI WCGNNNNNNNCGW 2 cut(s) 309, 360
PdiI GCCGGC 2 cut(s) 126, 144
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 4 cut(s) 31, 84, 196, 208
Ple19I CGATCG 1 cut(s) 36
PluTI GGCGCC 1 cut(s) 125
PmaCI CACGTG 2 cut(s) 278, 304
PmlI CACGTG 2 cut(s) 278, 304
Ppu21I YACGTR 2 cut(s) 278, 304
PspCI CACGTG 2 cut(s) 278, 304
PspN4I GGNNCC 1 cut(s) 123
PspXI VCTCGAGB 1 cut(s) 235
PvuI CGATCG 1 cut(s) 36
RgaI GCGATCGC 1 cut(s) 36
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 389, 402
SatI GCNGC 4 cut(s) 30, 83, 195, 207
Sau3AI GATC 3 cut(s) 33, 94, 298
ScrFI CCNGG 1 cut(s) 119
SetI ASST 6 cut(s) 84, 280, 306, 315, 332, 387
SfaAI GCGATCGC 1 cut(s) 36
SfoI GGCGCC 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 235
SgfI GCGATCGC 1 cut(s) 36
SgrAI CRCCGGYG 1 cut(s) 142
SlaI CTCGAG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 235, 388
SmoI CTYRAG 2 cut(s) 235, 388
Sse9I AATT 1 cut(s) 184
SspDI GGCGCC 1 cut(s) 121
StyD4I CCNGG 1 cut(s) 117
TaiI ACGT 4 cut(s) 280, 306, 315, 387
TaqI TCGA 1 cut(s) 236
TasI AATT 1 cut(s) 184
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 389, 402
Tru9I TTAA 2 cut(s) 389, 402
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 29, 82, 206
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 245
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 1 cut(s) 216
Vha464I CTTAAG 1 cut(s) 388
XapI RAATTY 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 235
XmnI GAANNNNTTC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.