Rmu_sc0000299.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000299.1
OG0016687
Physical Location & Seq
Forward (+)
67236 .. 67655
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000299.1_g000015.1.cds

Sequence Viewer

Length: 420 bp
atggggggttcagtaaaactcagcgcaacgatcacggcgttggtgatggtgatggcgatgggatcggaagcccgggagctgcggccgtccgatcacggcctggtgtacacgccggcggggaacacgtcggcggcgccggaggaggcgaagtcgttcttcagcggaggaggaggaggaggaggagggggagagggaggagagtcgggaggggtggcattgccgaaggcgatgaactcgaacgacacgtcgtggtgggagggagtgaacgcacgtgggcgggggagggatcacgtgagggacgtgctggttgtggtgagcttgatttgtgggattacaggtgtcgttctctttgtggcctccgcttttgtttatctcttgaggtaccgaaagcgcaaggcttcatctcttccttcttcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

14.23

Weight (kDa)

9.3

Isoelectric Point (pI)

33.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016687)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30515 AT4G21740
fragaria_vesca FvH4_3g10110 FvH4_3g10110
malus_domestica MD05G1272900.v1.1 MD10G1253000.v1.1
prunus_persica Prupe.4G090500_v2.0.a1
pyrus_communis pycom10g20870
rosa_chinensis RchiOBHm_Chr5g0016051
rosa_laevigata RLG00000032237
rosa_multiflora Rmu_sc0000299.1_g000015
rosa_roxburghii Rroxscaffold_1G00060600
rosa_rugosa Rorug05G0030900
rosa_wichuraiana Rw5G010770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 381
AccB1I GGYRCC 2 cut(s) 133, 381
AciI CCGC 6 cut(s) 82, 116, 131, 162, 277, 360
AclWI GGATC 2 cut(s) 70, 294
AcoI YGGCCR 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 142
AcvI CACGTG 2 cut(s) 272, 292
AcyI GRCGYC 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 249
AfaI GTAC 2 cut(s) 107, 383
AflIII ACRYGT 2 cut(s) 123, 243
AjiI CACGTC 3 cut(s) 126, 246, 301
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 2 cut(s) 79, 318
AluI AGCT 2 cut(s) 79, 318
AlwI GGATC 2 cut(s) 70, 294
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 3 cut(s) 83, 97, 354
ApeKI GCWGC 1 cut(s) 79
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 381
AspLEI GCGC 3 cut(s) 26, 136, 393
AsuC2I CCSGG 2 cut(s) 73, 74
AsuHPI GGTGA 3 cut(s) 55, 61, 325
AvaI CYCGRG 1 cut(s) 72
BanI GGYRCC 2 cut(s) 133, 381
BbrPI CACGTG 2 cut(s) 272, 292
BbvI GCAGC 1 cut(s) 66
BccI CCATC 3 cut(s) 40, 46, 52
BceAI ACGGC 3 cut(s) 51, 70, 112
BciT130I CCWGG 1 cut(s) 101
BcnI CCSGG 2 cut(s) 73, 74
BfoI RGCGCY 1 cut(s) 137
BisI GCNGC 3 cut(s) 80, 83, 132
BlsI GCNGC 3 cut(s) 81, 84, 133
Bme1390I CCNGG 3 cut(s) 73, 74, 101
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 3 cut(s) 126, 246, 301
BmiI GGNNCC 2 cut(s) 135, 383
BmrFI CCNGG 3 cut(s) 73, 74, 101
BpuEI CTTGAG 1 cut(s) 397
BpuMI CCSGG 2 cut(s) 73, 74
BsaAI YACGTR 2 cut(s) 272, 292
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 215
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 215
BseMII CTCAG 1 cut(s) 34
BseRI GAGGAG 8 cut(s) 155, 180, 183, 186, 189, 192, 195, 210
BseX3I CGGCCG 1 cut(s) 83
BseXI GCAGC 1 cut(s) 66
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 85, 99, 356
BshNI GGYRCC 2 cut(s) 133, 381
BsiEI CGRYCG 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 3 cut(s) 73, 113, 137
BslFI GGGAC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 3 cut(s) 85, 99, 356
BsoBI CYCGRG 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 4 cut(s) 30, 62, 91, 286
BspACI CCGC 6 cut(s) 82, 116, 131, 162, 277, 360
BspANI GGCC 3 cut(s) 85, 99, 356
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 135, 383
BspPI GGATC 2 cut(s) 70, 294
BspT107I GGYRCC 2 cut(s) 133, 381
BsrDI GCAATG 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 4 cut(s) 30, 62, 91, 286
BssNI GRCGYC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 134
BstAUI TGTACA 1 cut(s) 105
BstBAI YACGTR 2 cut(s) 272, 292
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 20
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 3 cut(s) 26, 136, 393
BstKTI GATC 4 cut(s) 33, 65, 94, 289
BstMBI GATC 4 cut(s) 30, 62, 91, 286
BstMCI CGRYCG 1 cut(s) 86
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 3 cut(s) 71, 72, 99
BstV1I GCAGC 1 cut(s) 66
BstZI CGGCCG 1 cut(s) 83
BsuRI GGCC 3 cut(s) 85, 99, 356
BtgZI GCGATG 2 cut(s) 71, 242
BtrI CACGTC 3 cut(s) 126, 246, 301
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 3 cut(s) 26, 136, 393
Cfr10I RCCGGY 1 cut(s) 112
Cfr9I CCCGGG 1 cut(s) 72
Csp6I GTAC 2 cut(s) 106, 382
CviJI RGCY 7 cut(s) 71, 79, 85, 99, 318, 356, 398
CviKI_1 RGCY 7 cut(s) 71, 79, 85, 99, 318, 356, 398
CviQI GTAC 2 cut(s) 106, 382
DdeI CTNAG 1 cut(s) 20
DinI GGCGCC 1 cut(s) 135
DpnI GATC 4 cut(s) 32, 64, 93, 288
DpnII GATC 4 cut(s) 30, 62, 91, 286
DraIII CACNNNGTG 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 83
EagI CGGCCG 1 cut(s) 83
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EclXI CGGCCG 1 cut(s) 83
Eco52I CGGCCG 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 142
Eco72I CACGTG 2 cut(s) 272, 292
Eco88I CYCGRG 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 99
EgeI GGCGCC 1 cut(s) 135
EheI GGCGCC 1 cut(s) 135
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 1 cut(s) 311
FauI CCCGC 2 cut(s) 109, 270
Fnu4HI GCNGC 3 cut(s) 80, 83, 132
Fsp4HI GCNGC 3 cut(s) 80, 83, 132
GlaI GCGC 3 cut(s) 25, 135, 392
GluI GCNGC 3 cut(s) 80, 83, 132
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 3 cut(s) 85, 99, 356
HapII CCGG 3 cut(s) 73, 113, 137
HhaI GCGC 3 cut(s) 26, 136, 393
Hin1I GRCGYC 1 cut(s) 134
Hin6I GCGC 3 cut(s) 24, 134, 391
HinP1I GCGC 3 cut(s) 24, 134, 391
HinfI GANTC 1 cut(s) 200
HpaII CCGG 3 cut(s) 73, 113, 137
HphI GGTGA 3 cut(s) 55, 61, 325
Hpy166II GTNNAC 3 cut(s) 106, 108, 265
Hpy188I TCNGA 2 cut(s) 67, 91
Hpy188III TCNNGA 3 cut(s) 204, 376, 417
Hpy8I GTNNAC 3 cut(s) 106, 108, 265
Hpy99I CGWCG 2 cut(s) 130, 250
HpyAV CCTTC 2 cut(s) 217, 420
HpyCH4IV ACGT 5 cut(s) 125, 245, 271, 291, 300
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 5 cut(s) 125, 245, 271, 291, 300
Hsp92I GRCGYC 1 cut(s) 134
HspAI GCGC 3 cut(s) 24, 134, 391
KasI GGCGCC 1 cut(s) 133
KpnI GGTACC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 30, 62, 91, 286
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 7 cut(s) 86, 86, 113, 126, 150, 290, 321
Lsp1109I GCAGC 1 cut(s) 66
MaeII ACGT 5 cut(s) 125, 245, 271, 291, 300
MalI GATC 4 cut(s) 32, 64, 93, 288
MboI GATC 4 cut(s) 30, 62, 91, 286
MboII GAAGA 3 cut(s) 148, 398, 405
Mly113I GGCGCC 1 cut(s) 134
MlyI GAGTC 1 cut(s) 209
MreI CGCCGGCG 1 cut(s) 112
MroNI GCCGGC 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 3 cut(s) 73, 113, 137
MspR9I CCNGG 3 cut(s) 73, 74, 101
MvaI CCWGG 1 cut(s) 101
NaeI GCCGGC 1 cut(s) 114
NarI GGCGCC 1 cut(s) 134
NciI CCSGG 2 cut(s) 73, 74
NdeII GATC 4 cut(s) 30, 62, 91, 286
NgoMIV GCCGGC 1 cut(s) 112
NlaIV GGNNCC 2 cut(s) 135, 383
PcsI WCGNNNNNNNCGW 3 cut(s) 35, 242, 297
PdiI GCCGGC 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 81, 84, 133
PleI GAGTC 1 cut(s) 208
PluTI GGCGCC 1 cut(s) 137
PmaCI CACGTG 2 cut(s) 272, 292
PmlI CACGTG 2 cut(s) 272, 292
PpsI GAGTC 1 cut(s) 208
Ppu21I YACGTR 2 cut(s) 272, 292
Psp6I CCWGG 1 cut(s) 99
PspCI CACGTG 2 cut(s) 272, 292
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 135, 383
RsaI GTAC 2 cut(s) 107, 383
RsaNI GTAC 2 cut(s) 106, 382
SatI GCNGC 3 cut(s) 80, 83, 132
Sau3AI GATC 4 cut(s) 30, 62, 91, 286
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 3 cut(s) 73, 74, 101
SetI ASST 9 cut(s) 81, 128, 248, 274, 294, 303, 320, 340, 383
SfoI GGCGCC 1 cut(s) 135
SgrAI CRCCGGYG 1 cut(s) 112
SmaI CCCGGG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
SsiI CCGC 6 cut(s) 82, 116, 131, 162, 277, 360
SspDI GGCGCC 1 cut(s) 133
StyD4I CCNGG 3 cut(s) 71, 72, 99
TaiI ACGT 5 cut(s) 128, 248, 274, 294, 303
TaqI TCGA 1 cut(s) 236
TatI WGTACW 1 cut(s) 105
TauI GCSGC 2 cut(s) 85, 134
TseI GCWGC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 245, 390
TspMI CCCGGG 1 cut(s) 72
XmaI CCCGGG 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.