MD10G1253000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016687
Physical Location & Seq
Forward (+)
34569239 .. 34569673
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1253000.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGCGTTTTTCATCGAAATTAGTGTAGCAGCGATCGCGGTGTTTCTGATTGCGGTGGCGATGGAATCGGACGGCCGAGAGCTGCGGCCGTCCGATCACGGCCTCGTGTACCAGGACCAGGCGCCGGCGAGCGCGAAGTCGCCGACGGAAGAGAAGTCGTTCTTCGTCGGGGGAGGAGGAGGAGAGAATTCGCCGGCGGCGGGTGACGCGGCACTGCCGAAGCCGAAGGCGATGAACTCGAGCGACGAGTCGTGGTGGAAGGGCGTGGTGGCACGTGACGGTGGGCGGGGTAGAGATCTCGTGAGGGACGTGCTGCTGGTGGCGAGCTTAATTTGTGGGATTGCCGGCGTGGCTCTGTTTGTGGCCTCTGCTTTCGTTTACGTCTTAAGGTACCGAAAGCATAAATCTTTACCGTCAGAAGCTCCGTCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

15.14

Weight (kDa)

6.1

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016687)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30515 AT4G21740
fragaria_vesca FvH4_3g10110 FvH4_3g10110
malus_domestica MD05G1272900.v1.1 MD10G1253000.v1.1
prunus_persica Prupe.4G090500_v2.0.a1
pyrus_communis pycom10g20870
rosa_chinensis RchiOBHm_Chr5g0016051
rosa_laevigata RLG00000032237
rosa_multiflora Rmu_sc0000299.1_g000015
rosa_roxburghii Rroxscaffold_1G00060600
rosa_rugosa Rorug05G0030900
rosa_wichuraiana Rw5G010770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 2 cut(s) 121, 390
AccII CGCG 3 cut(s) 38, 134, 209
AciI CCGC 7 cut(s) 38, 53, 85, 197, 200, 209, 286
AcoI YGGCCR 2 cut(s) 73, 86
AcsI RAATTY 1 cut(s) 187
AcvI CACGTG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 110, 392
AfiI CCNNNNNNNGG 3 cut(s) 118, 124, 200
AflII CTTAAG 1 cut(s) 385
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 2 cut(s) 111, 117
AluBI AGCT 3 cut(s) 82, 327, 422
AluI AGCT 3 cut(s) 82, 327, 422
Ama87I CYCGRG 1 cut(s) 238
AoxI GGCC 4 cut(s) 73, 86, 100, 363
ApeKI GCWGC 3 cut(s) 29, 82, 313
ApoI RAATTY 1 cut(s) 187
AsiSI GCGATCGC 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 158
Asp718I GGTACC 1 cut(s) 390
AspLEI GCGC 2 cut(s) 124, 134
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 1 cut(s) 115
BanI GGYRCC 2 cut(s) 121, 390
BauI CACGAG 2 cut(s) 104, 299
BbrPI CACGTG 1 cut(s) 275
BbvI GCAGC 3 cut(s) 41, 69, 300
BccI CCATC 1 cut(s) 55
BceAI ACGGC 3 cut(s) 73, 88, 115
BciT130I CCWGG 2 cut(s) 113, 119
BfoI RGCGCY 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 350
BglII AGATCT 1 cut(s) 295
BisI GCNGC 6 cut(s) 30, 83, 86, 198, 210, 314
BlsI GCNGC 6 cut(s) 31, 84, 87, 199, 211, 315
Bme1390I CCNGG 2 cut(s) 113, 119
Bme18I GGWCC 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 2 cut(s) 123, 392
BmrFI CCNGG 2 cut(s) 113, 119
BsaAI YACGTR 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 122
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 124, 200
Bse118I RCCGGY 3 cut(s) 124, 193, 344
BseBI CCWGG 2 cut(s) 113, 119
BseLI CCNNNNNNNGG 3 cut(s) 118, 124, 200
BseRI GAGGAG 3 cut(s) 189, 192, 195
BseX3I CGGCCG 2 cut(s) 73, 86
BseXI GCAGC 3 cut(s) 41, 69, 300
Bsh1236I CGCG 3 cut(s) 38, 134, 209
Bsh1285I CGRYCG 3 cut(s) 36, 76, 89
BshFI GGCC 4 cut(s) 75, 88, 102, 365
BshNI GGYRCC 2 cut(s) 121, 390
BsiEI CGRYCG 3 cut(s) 36, 76, 89
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 3 cut(s) 125, 194, 345
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 118, 124, 200
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 4 cut(s) 75, 88, 102, 365
BsoBI CYCGRG 1 cut(s) 238
Bsp143I GATC 3 cut(s) 33, 94, 295
BspACI CCGC 7 cut(s) 38, 53, 85, 197, 200, 209, 286
BspANI GGCC 4 cut(s) 75, 88, 102, 365
BspFNI CGCG 3 cut(s) 38, 134, 209
BspLI GGNNCC 2 cut(s) 123, 392
BspT107I GGYRCC 2 cut(s) 121, 390
BspTI CTTAAG 1 cut(s) 385
BsrFI RCCGGY 3 cut(s) 124, 193, 344
BssAI RCCGGY 3 cut(s) 124, 193, 344
BssMI GATC 3 cut(s) 33, 94, 295
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 2 cut(s) 104, 299
Bst2BI CACGAG 2 cut(s) 104, 299
Bst2UI CCWGG 2 cut(s) 113, 119
Bst4CI ACNGT 2 cut(s) 281, 414
Bst6I CTCTTC 1 cut(s) 144
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 385
BstBAI YACGTR 1 cut(s) 275
BstC8I GCNNGC 5 cut(s) 126, 130, 195, 325, 346
BstFNI CGCG 3 cut(s) 38, 134, 209
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 2 cut(s) 124, 134
BstKTI GATC 3 cut(s) 36, 97, 298
BstMBI GATC 3 cut(s) 33, 94, 295
BstMCI CGRYCG 3 cut(s) 36, 76, 89
BstMWI GCNNNNNNNGC 3 cut(s) 35, 206, 350
BstNI CCWGG 2 cut(s) 113, 119
BstSCI CCNGG 2 cut(s) 111, 117
BstUI CGCG 3 cut(s) 38, 134, 209
BstV1I GCAGC 3 cut(s) 41, 69, 300
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BstZI CGGCCG 2 cut(s) 73, 86
BsuRI GGCC 4 cut(s) 75, 88, 102, 365
BtgZI GCGATG 2 cut(s) 74, 245
BtrI CACGTC 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 212
Cac8I GCNNGC 5 cut(s) 126, 130, 195, 325, 346
CfoI GCGC 2 cut(s) 124, 134
Cfr10I RCCGGY 3 cut(s) 124, 193, 344
Cfr13I GGNCC 1 cut(s) 115
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 109, 391
CviJI RGCY 9 cut(s) 75, 82, 88, 102, 223, 327, 353, 365, 422
CviKI_1 RGCY 9 cut(s) 75, 82, 88, 102, 223, 327, 353, 365, 422
CviQI GTAC 2 cut(s) 109, 391
DinI GGCGCC 1 cut(s) 123
DpnI GATC 3 cut(s) 35, 96, 297
DpnII GATC 3 cut(s) 33, 94, 295
EaeI YGGCCR 2 cut(s) 73, 86
EagI CGGCCG 2 cut(s) 73, 86
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
EclXI CGGCCG 2 cut(s) 73, 86
Eco47I GGWCC 1 cut(s) 115
Eco52I CGGCCG 2 cut(s) 73, 86
Eco72I CACGTG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 238
EcoRI GAATTC 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 111, 117
EgeI GGCGCC 1 cut(s) 123
EheI GGCGCC 1 cut(s) 123
FaiI YATR 1 cut(s) 402
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 320
FauI CCCGC 2 cut(s) 193, 279
Fnu4HI GCNGC 6 cut(s) 30, 83, 86, 198, 210, 314
Fsp4HI GCNGC 6 cut(s) 30, 83, 86, 198, 210, 314
GlaI GCGC 2 cut(s) 123, 133
GluI GCNGC 6 cut(s) 30, 83, 86, 198, 210, 314
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 4 cut(s) 75, 88, 102, 365
HapII CCGG 3 cut(s) 125, 194, 345
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 124, 134
Hin1I GRCGYC 1 cut(s) 122
Hin6I GCGC 2 cut(s) 122, 132
HinP1I GCGC 2 cut(s) 122, 132
HinfI GANTC 2 cut(s) 65, 248
HpaII CCGG 3 cut(s) 125, 194, 345
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 109, 379
Hpy188I TCNGA 4 cut(s) 48, 70, 94, 418
Hpy188III TCNNGA 2 cut(s) 301, 432
Hpy8I GTNNAC 2 cut(s) 109, 379
Hpy99I CGWCG 3 cut(s) 148, 170, 248
HpyAV CCTTC 2 cut(s) 220, 253
HpyCH4III ACNGT 2 cut(s) 281, 414
HpyCH4IV ACGT 3 cut(s) 274, 309, 381
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 206, 350
HpySE526I ACGT 3 cut(s) 274, 309, 381
Hsp92I GRCGYC 1 cut(s) 122
HspAI GCGC 2 cut(s) 122, 132
KasI GGCGCC 1 cut(s) 121
KpnI GGTACC 1 cut(s) 394
KroI GCCGGC 3 cut(s) 124, 193, 344
KroNI GCCGGC 3 cut(s) 126, 195, 346
Kzo9I GATC 3 cut(s) 33, 94, 295
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 8 cut(s) 98, 104, 125, 131, 138, 207, 302, 358
Lsp1109I GCAGC 3 cut(s) 41, 69, 300
MaeII ACGT 3 cut(s) 274, 309, 381
MaeIII GTNAC 2 cut(s) 203, 275
MalI GATC 3 cut(s) 35, 96, 297
MboI GATC 3 cut(s) 33, 94, 295
MboII GAAGA 2 cut(s) 154, 161
MflI RGATCY 1 cut(s) 295
MluCI AATT 3 cut(s) 18, 187, 330
Mly113I GGCGCC 1 cut(s) 122
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 6 cut(s) 113, 167, 170, 173, 297, 376
MreI CGCCGGCG 2 cut(s) 124, 193
MroNI GCCGGC 3 cut(s) 124, 193, 344
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 2 cut(s) 329, 386
MspCI CTTAAG 1 cut(s) 385
MspI CCGG 3 cut(s) 125, 194, 345
MspR9I CCNGG 2 cut(s) 113, 119
MvaI CCWGG 2 cut(s) 113, 119
MvnI CGCG 3 cut(s) 38, 134, 209
MwoI GCNNNNNNNGC 3 cut(s) 35, 206, 350
NaeI GCCGGC 3 cut(s) 126, 195, 346
NarI GGCGCC 1 cut(s) 122
NdeII GATC 3 cut(s) 33, 94, 295
NgoMIV GCCGGC 3 cut(s) 124, 193, 344
NlaIV GGNNCC 2 cut(s) 123, 392
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 2 cut(s) 203, 275
PaeR7I CTCGAG 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdiI GCCGGC 3 cut(s) 126, 195, 346
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 6 cut(s) 31, 84, 87, 199, 211, 315
Ple19I CGATCG 1 cut(s) 36
PleI GAGTC 1 cut(s) 256
PluTI GGCGCC 1 cut(s) 125
PmaCI CACGTG 1 cut(s) 275
PmlI CACGTG 1 cut(s) 275
PpsI GAGTC 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 111, 117
PspCI CACGTG 1 cut(s) 275
PspGI CCWGG 2 cut(s) 111, 117
PspN4I GGNNCC 2 cut(s) 123, 392
PspPI GGNCC 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 238
PsuI RGATCY 1 cut(s) 295
PvuI CGATCG 1 cut(s) 36
RgaI GCGATCGC 1 cut(s) 36
RsaI GTAC 2 cut(s) 110, 392
RsaNI GTAC 2 cut(s) 109, 391
SaqAI TTAA 2 cut(s) 329, 386
SatI GCNGC 6 cut(s) 30, 83, 86, 198, 210, 314
Sau3AI GATC 3 cut(s) 33, 94, 295
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 113, 119
SetI ASST 7 cut(s) 84, 277, 312, 329, 384, 392, 424
SfaAI GCGATCGC 1 cut(s) 36
SfoI GGCGCC 1 cut(s) 123
Sfr274I CTCGAG 1 cut(s) 238
SgfI GCGATCGC 1 cut(s) 36
SgrAI CRCCGGYG 2 cut(s) 124, 193
SinI GGWCC 1 cut(s) 115
SlaI CTCGAG 1 cut(s) 238
SmlI CTYRAG 2 cut(s) 238, 385
SmoI CTYRAG 2 cut(s) 238, 385
Sse9I AATT 3 cut(s) 18, 187, 330
SsiI CCGC 7 cut(s) 38, 53, 85, 197, 200, 209, 286
SspDI GGCGCC 1 cut(s) 121
StyD4I CCNGG 2 cut(s) 111, 117
TaaI ACNGT 2 cut(s) 281, 414
TaiI ACGT 3 cut(s) 277, 312, 384
TaqI TCGA 2 cut(s) 15, 239
TasI AATT 3 cut(s) 18, 187, 330
TauI GCSGC 3 cut(s) 88, 200, 212
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 329, 386
Tru9I TTAA 2 cut(s) 329, 386
TscAI CASTG 1 cut(s) 219
TseFI GTSAC 2 cut(s) 203, 275
TseI GCWGC 3 cut(s) 29, 82, 313
Tsp45I GTSAC 2 cut(s) 203, 275
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 2 cut(s) 161, 414
TspRI CASTG 1 cut(s) 219
Vha464I CTTAAG 1 cut(s) 385
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 187
XhoI CTCGAG 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.