MD05G1273800.v1.1

kxDL motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009507
Physical Location & Seq
Forward (+)
40866416 .. 40868568
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1273800.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGAACAGACGGAGAAAGAAACGATCAGGGAAGCGTCAGAGGAGATATCGAATGACTTCAAAACACTAATCGATGCTCAGGACTTGGATTCCCTCAAACAATTGCAGCACATCATATTGGGGAGGTTGCAAGATGGAAATGCAGTCCTGTCACATTTTAATGAGTTTTCAGAGAACAGTTTTGCTGAGGTTTCTGCAGATTTCTCGAGAAACACACGCCGTTTAAAGTCTATGAAGACTGATCTTGAACACATATTTCAGAAGTTAAGGAGCATGAAGTCAAGAATTTTGGCTACCTATCCAGATGCATTTCCAGATGACTCGAAACAAGTACTCGATCGAAGGCCGGACCTTGAAACGCCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KxDL

Protein Analysis

123

Amino Acids

14.14

Weight (kDa)

5.05

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KxDL PF10241 20 - 104 9.4e-24 Uncharacterized conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 1 cut(s) 333
AgsI TTSAA 3 cut(s) 61, 248, 356
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 118
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 204
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 349
BmsI GCATC 2 cut(s) 64, 295
BpiI GAAGAC 1 cut(s) 242
Bpu10I CCTNAGC 2 cut(s) 78, 186
Bsa29I ATCGAT 1 cut(s) 72
BseCI ATCGAT 1 cut(s) 72
BseMII CTCAG 2 cut(s) 92, 177
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 118
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 340
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 347
BsnI GGCC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 205
Bsp143I GATC 3 cut(s) 24, 241, 337
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 2 cut(s) 91, 178
BspDI ATCGAT 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 199
BssMI GATC 3 cut(s) 24, 241, 337
Bst4CI ACNGT 1 cut(s) 179
BstDEI CTNAG 2 cut(s) 78, 186
BstKTI GATC 3 cut(s) 27, 244, 340
BstMBI GATC 3 cut(s) 24, 241, 337
BstMCI CGRYCG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 349
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 1 cut(s) 24
Csp6I GTAC 1 cut(s) 332
CviAII CATG 1 cut(s) 274
CviJI RGCY 2 cut(s) 293, 346
CviKI_1 RGCY 2 cut(s) 293, 346
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 2 cut(s) 78, 186
DpnI GATC 3 cut(s) 26, 243, 339
DpnII GATC 3 cut(s) 24, 241, 337
DraI TTTAAA 1 cut(s) 225
Eco32I GATATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 1 cut(s) 277
FaiI YATR 4 cut(s) 116, 233, 254, 275
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 107
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 24
Hin1II CATG 1 cut(s) 277
HinfI GANTC 2 cut(s) 89, 320
HpaII CCGG 1 cut(s) 347
Hpy188I TCNGA 3 cut(s) 40, 172, 261
Hpy188III TCNNGA 7 cut(s) 80, 205, 207, 245, 282, 302, 314
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 106, 130, 143, 197, 308
HpyF3I CTNAG 2 cut(s) 78, 186
Hsp92II CATG 1 cut(s) 277
Kzo9I GATC 3 cut(s) 24, 241, 337
LmnI GCTCC 1 cut(s) 270
LpnPI CCDG 6 cut(s) 13, 65, 161, 315, 327, 360
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 2 cut(s) 64, 295
MaeIII GTNAC 1 cut(s) 150
MalI GATC 3 cut(s) 26, 243, 339
MboI GATC 3 cut(s) 24, 241, 337
MboII GAAGA 1 cut(s) 247
MfeI CAATTG 1 cut(s) 101
MluCI AATT 2 cut(s) 101, 285
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 4 cut(s) 34, 104, 117, 181
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 3 cut(s) 159, 224, 266
MslI CAYNNNNRTG 1 cut(s) 159
MspI CCGG 1 cut(s) 347
MunI CAATTG 1 cut(s) 101
NdeII GATC 3 cut(s) 24, 241, 337
NlaIII CATG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 310
PaeR7I CTCGAG 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 108
Ple19I CGATCG 1 cut(s) 340
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 349
PstI CTGCAG 1 cut(s) 199
PvuI CGATCG 1 cut(s) 340
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 159, 224, 266
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 3 cut(s) 24, 241, 337
Sau96I GGNCC 1 cut(s) 349
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 1 cut(s) 314
SetI ASST 4 cut(s) 128, 192, 299, 354
SfaNI GCATC 2 cut(s) 64, 295
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 349
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 2 cut(s) 101, 285
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 6 cut(s) 50, 72, 206, 323, 336, 340
TasI AATT 2 cut(s) 101, 285
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 159, 224, 266
Tru9I TTAA 3 cut(s) 159, 224, 266
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 2 cut(s) 248, 290
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 1 cut(s) 285
XhoI CTCGAG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 56
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.