Rorug05G0030300

kxDL motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009507
Physical Location & Seq
Reverse (-)
1914336 .. 1928584
14249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0030300.1

Sequence Viewer

Length: 3477 bp
ATGATCTCTTCAGTTAGGCTCAGAACCCCAACTTTCACGTCGGCACAGTTCTCGAACCAGAAGTGTTGTTTGGGAAAGATAGAGTATTGTTCCCTCCCTAATATTCAGAATGCGAAACAGAGCATCCTCTGTTCCTCCAAGCCGCTTCACATTTCTTCAGCCGAGAGTTTTGCATTGGTGGCCAAGACCCAGAAGCGCGGTTCGACAAGGTGTGATGTCTATGAGGCAGACAGATCACGCCCGATTGAGATCAACATCGACCTACCGGACCAGCAGGCTCGGAAGGAGACAGCTCAGACTCTCAAGATTAGTCTTTACTTTGCGACCTGGTGGGCTTTGAATGTTGTGTTCAATATATACAACAAGAAGGTTTTGAATGCGTTCCCATTTCCATGGCTCACATCCACGTTGTCACTCGCAACTGGGTCTCTGATGATGCTCATTTCTTGGGCCGTTGGGATTGCTGAAGCCCCCAAAACAGATATGGACTTCTGGAAGACATTGTTTCCGGTTGCCGTGGCACATTCAATTGGTCATGTAGCAGCGACTGTGAGTATGTCAAAGGTTGCAGTTTCATTCACGCATATCATCAAGAGTGGTGAACCTGCTTTCAGTGTTTTGGTTTCAAGGTTCTTGTTAGGCGAAACGTTTCCCAGTTCGGTTTACCTCTCACTGTTGCCAATTATCGGAGGGTGTGCGCTTGCTGCTGTAACCGAGATCAATTTCAACATGATTGGGTTTATGGGTGCTATGATATCGAATCTGGCATTCGTGTTCCGAAACATCTTTTCGAAGAAAGGGATGAAGGGGAAGTCTGTTAGTGGCATGAACTACTATGCTTGTCTGTCTATGTTGTCTCTATTGATCCTGATTCCGTTTGCCATTGCCGTCGAGGGTCCCCAGTTATGGGCAGCTGGATGGAAGACAGCTCTTGCACAGATTGGTCCCAATTTCATATGGTGGCTGGCAGCTCAGAGTATCTTCTATCACTTGTATAATCAGGTTTCATACATGTCACTCGATCAGATTTCTCCCTTAACATTCAGCATCGGGAACACAATGAAGCGTATATCCGTCATAGTTTCCTCCATTATCATTTTCCGCACCCCTGTTCAACCGGTCAATTGCCTTGGAGCAGGCATTGCAATCCTTGGCACCTTCCTCTACTCACAGGCCAGGACTCAGGAGATTCTGCTTATAATTGGTTCAATTGCTTCCAAATTTTTCCAATCATGCAACGTTTGTCAAGCTAATTATGATTTCAGTTTTTCCTCACCGTTGATGTCACACCCACGTAGGCTTCACTGCACAATAGTTGCATCAAAAGTGTCATGTAATCACATATCCTTGAAAGAAAACTCGCCGAGCAACTTTCTCTTTGGAACTGCATCCTCTTCTTATCAGTTTGAAGGAGCTTTTCTGAATGATGGTAAAGGTCTCAACAACTGGGATATCTTCACTCACAAGCCTGGTACTATCCTGGATGGAACTAATGGAGACATTGCTGTTGATCAGTACCATCTTTATCAGGAAGACTTGGATCTCATGAATAACATTAGAGTCAACAGCTACCGTTTTTCTATATCATGGGCAAGAATTTTACCCAAAGGAAGATTTGGAAAAGTGAACAGAGCTGGCATTGATCACTATAACAAGTTCATCGATGCTCTTCTGCTTAGAGGAATCCAGCCCTTTGTGACATTGACTCACTACGACATACCTCAGGAACTTGAAGACAGATATGGATCTTGGCTAAGTCCCCTACTGCAGGACGATTTTAGATATTATGCAAATATATGTTTCAAATTCTTTGGAGACAGGGTCAAGTACTGGTCCACTTTCAATGAGCCCAATGTTGTGGTCATTCGTGGATATAGGTCAGGGATTTACCCACCATCTCATTGCTCTCTCCCTTTCGGAAATTGTACTACTGGAGACTCAGAAAGAGAGCCTTTCATTGCAGCTCATAACATCATTCTATCCCATGCAGCTGCTGTGAATATATACAGAACCAAATATCAGAAAAAACAAGGAGGTAGCATTGGGATGGTGATGAATGCTGTATGGTATGAACCCATCAGCAACTCCTTAGAAGACAGATTAGCAGCTGAGAGGGCTCAGTCTTTCTATATCAACTGGTTCTTAGACCCAATTCTCCATGGAAGATACCCTGCAGAAATGCATGAGCTTCTAGGAGCTGATTTGCCTGTATTTTCCAAATTTGATCTGGAGATGCTGAAGAATGGATTAGACTTCATCGGTATCAATCACTACACCAGTTTTTACTCAAAAGACTGTATGTTTTCTCCATGTGAACCAGGACCAGGAGCTTCAAGGACAGAGGGTTATGCTTTGAGAACTGCACAAAAAGATGGAGTCTACATTGGAGAACCAACTTCAGTTGATTGGATATATGTGTATCCTCAAGGAATGGAGAAGATGGTAACATACATAAAAGAGAGGTACAATAATACACCAATGTTTATTACAGAAAATGGGTATGGTGTGGAGGAGAATCCAAATTCCACAAATGAAGAACAGTTCCATGATGTCAAGAGAGTGGAGTACTTGAGAAGTTACTTACAGGCACTAGCAGATACAATGAGAACTGAAGGAGCAGATGTAAGAGGGTACTTCGTGTGGTCATTGCTTGATAATTTCGAGTGGACAAGTGGATATACGGTCAAGTTTGGACTTCACCATGTTGATTATGGCACTCTCAAGAGAACTCCAAGACTCTCTGCAGCTTGTAATGTCACTCCTAATCATTTAGTTCACAGACATGTAGGCCTCACGTACTGCACAATAGTTGCATCAAGAGTGTCATGTGATCACATATCCTTGAAAGGAAACTCACCGAGCAAATTTCTCTTTGGAACTGCATCCTCTTCTTATCAGTTTGAAGGTGCTTTTCTGAAGGATGGTAAAGGTCTCAACAACTGGGATGTCTTCACTCACAAGCCTGGTACTATCCTGGATGGAACTAATGGAGACATTGCTGTTGATCAGTACCATCTTTATCAGGAAGACTTGGATCTCATGAATAACATTGGAGTAACCAGCTACCGCTTTTCTATATCATGGGCAAGAATTTTACCCAAAGGAAGATTTGGAAAAGTGAACAGAGCTGGCATTGATCACTATAACAAGTTCATCGATGCTCTTCTGCTTAGAGGAATCCAGCCCTTTGTGACATTGAGTCACTACTACGTACCTCAGGATCTTGAAGACAGATATGGATCTTGGCCAAGTCCCCTACTGCAGTACACAGCAAATGTTCCAAAAAAACAAGGAGGTAGCATTGGAATAGTGATGAGTGCTGTATGGTATGAACCCATCAGCAACTCCTTAGAAGACAAATTAGCAGCTGAGAGAGCTCAGTCTTTCTTTATGAACTGGTTCTTAGACCCAATTATCCATGGAAGATACCCTGCAGAAATGCATGAGCTTCTAGGAGCTGATTTGCCTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KxDL

Protein Analysis

1158

Amino Acids

130.21

Weight (kDa)

8.18

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 101 - 390 2.3e-114 Triose-phosphate Transporter family
EamA PF00892 110 - 235 8.8e-06 EamA-like transporter family
Glyco_hydro_1 PF00232 452 - 915 1.2e-148 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 952 - 1084 2.5e-54 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 1096 - 1152 6e-10 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1199
Acc36I ACCTGC 1 cut(s) 613
AccB1I GGYRCC 1 cut(s) 1154
AccI GTMKAC 1 cut(s) 2379
AccII CGCG 1 cut(s) 198
AciI CCGC 4 cut(s) 143, 198, 1102, 3067
AclI AACGTT 2 cut(s) 647, 1239
AclWI GGATC 6 cut(s) 859, 1548, 1753, 3042, 3228, 3247
AcoI YGGCCR 2 cut(s) 180, 3245
AcsI RAATTY 7 cut(s) 1220, 1596, 1805, 2219, 2521, 2864, 3090
AcuI CTGAAG 6 cut(s) 141, 486, 2258, 2382, 2631, 2936
AfiI CCNNNNNNNGG 5 cut(s) 686, 906, 907, 1768, 2324
AflIII ACRYGT 2 cut(s) 1011, 2782
AgeI ACCGGT 1 cut(s) 1117
AhdI GACNNNNNGTC 1 cut(s) 3198
AjiI CACGTC 1 cut(s) 39
AjnI CCWGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
Alw21I GWGCWC 1 cut(s) 3379
Alw26I GTCTC 9 cut(s) 281, 432, 861, 1442, 1491, 1809, 1929, 2936, 2985
AlwI GGATC 6 cut(s) 859, 1548, 1753, 3042, 3228, 3247
AlwNI CAGNNNCTG 2 cut(s) 548, 1994
AoxI GGCC 5 cut(s) 180, 450, 1173, 2788, 3245
ApoI RAATTY 7 cut(s) 1220, 1596, 1805, 2219, 2521, 2864, 3090
AsiGI ACCGGT 1 cut(s) 1117
Asp700I GAANNNNTTC 3 cut(s) 380, 648, 3398
AspLEI GCGC 2 cut(s) 198, 700
AspS9I GGNCC 6 cut(s) 268, 450, 896, 944, 1833, 2321
AsuHPI GGTGA 5 cut(s) 611, 1266, 2062, 2690, 2847
AsuII TTCGAA 1 cut(s) 791
AvaII GGWCC 5 cut(s) 268, 896, 944, 1833, 2321
AxyI CCTNAGG 2 cut(s) 1722, 3216
BalI TGGCCA 2 cut(s) 182, 3247
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 3 cut(s) 1851, 2119, 3379
BbsI GAAGAC 9 cut(s) 503, 929, 1539, 1740, 2100, 2941, 3033, 3234, 3360
Bbv12I GWGCWC 1 cut(s) 3379
BceAI ACGGC 3 cut(s) 437, 500, 872
BcgI CGANNNNNNTGC 4 cut(s) 33, 67, 152, 186
BciT130I CCWGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
BciVI GTATCC 1 cut(s) 2430
BclI TGATCA 5 cut(s) 1510, 1642, 2830, 3004, 3136
BcoDI GTCTC 9 cut(s) 281, 432, 861, 1442, 1491, 1809, 1929, 2936, 2985
BfaI CTAG 3 cut(s) 2192, 2591, 3452
BfmI CTRYAG 5 cut(s) 1766, 2172, 2742, 3260, 3432
BfuAI ACCTGC 1 cut(s) 613
BfuI GTATCC 1 cut(s) 2430
BmcAI AGTACT 2 cut(s) 1829, 2567
Bme1390I CCNGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
Bme18I GGWCC 5 cut(s) 268, 896, 944, 1833, 2321
BmeRI GACNNNNNGTC 1 cut(s) 3198
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 6 cut(s) 268, 450, 896, 944, 1833, 2321
BmiI GGNNCC 4 cut(s) 897, 898, 946, 1156
BmrFI CCNGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
BmrI ACTGGG 5 cut(s) 432, 648, 895, 1456, 2950
BmuI ACTGGG 5 cut(s) 432, 648, 895, 1456, 2950
BpiI GAAGAC 9 cut(s) 503, 929, 1539, 1740, 2100, 2941, 3033, 3234, 3360
BpmI CTGGAG 2 cut(s) 1953, 2249
Bpu14I TTCGAA 1 cut(s) 791
BpuEI CTTGAG 4 cut(s) 287, 2409, 2590, 2705
Bsa29I ATCGAT 2 cut(s) 1662, 3156
BsaAI YACGTR 3 cut(s) 1295, 2796, 3211
BsaBI GATNNNNATC 4 cut(s) 248, 254, 1744, 3238
BsaI GGTCTC 3 cut(s) 432, 1442, 2936
BsaJI CCNNGG 6 cut(s) 392, 516, 1129, 1150, 2158, 3418
BsaWI WCCGGW 3 cut(s) 265, 508, 1117
BsaXI ACNNNNNCTCC 4 cut(s) 1806, 1836, 2607, 2637
Bsc4I CCNNNNNNNGG 5 cut(s) 686, 906, 907, 1768, 2324
Bse118I RCCGGY 1 cut(s) 1117
Bse21I CCTNAGG 2 cut(s) 1722, 3216
Bse3DI GCAATG 7 cut(s) 882, 1140, 1500, 1900, 1956, 2645, 2994
Bse8I GATNNNNATC 4 cut(s) 248, 254, 1744, 3238
BseBI CCWGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
BseCI ATCGAT 2 cut(s) 1662, 3156
BseDI CCNNGG 6 cut(s) 392, 516, 1129, 1150, 2158, 3418
BseJI GATNNNNATC 4 cut(s) 248, 254, 1744, 3238
BseLI CCNNNNNNNGG 5 cut(s) 686, 906, 907, 1768, 2324
BseMI GCAATG 7 cut(s) 882, 1140, 1500, 1900, 1956, 2645, 2994
BseRI GAGGAG 1 cut(s) 2525
BsgI GTGCAG 3 cut(s) 1291, 2346, 2785
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 5 cut(s) 182, 452, 1175, 2790, 3247
BshNI GGYRCC 1 cut(s) 1154
BshTI ACCGGT 1 cut(s) 1117
BshVI ATCGAT 2 cut(s) 1662, 3156
BsiHKAI GWGCWC 1 cut(s) 3379
BsiSI CCGG 3 cut(s) 266, 509, 1118
BslFI GGGAC 4 cut(s) 882, 930, 1743, 3237
BslI CCNNNNNNNGG 5 cut(s) 686, 906, 907, 1768, 2324
BsmAI GTCTC 9 cut(s) 281, 432, 861, 1442, 1491, 1809, 1929, 2936, 2985
BsmFI GGGAC 4 cut(s) 882, 930, 1743, 3237
BsmI GAATGC 4 cut(s) 115, 382, 767, 2062
BsnI GGCC 5 cut(s) 182, 452, 1175, 2790, 3247
Bso31I GGTCTC 3 cut(s) 432, 1442, 2936
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 3 cut(s) 1851, 2119, 3379
Bsp19I CCATGG 3 cut(s) 392, 2158, 3418
BspACI CCGC 4 cut(s) 143, 198, 1102, 3067
BspANI GGCC 5 cut(s) 182, 452, 1175, 2790, 3247
BspDI ATCGAT 2 cut(s) 1662, 3156
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 2 cut(s) 1545, 3039
BspLI GGNNCC 4 cut(s) 897, 898, 946, 1156
BspMAI CTGCAG 5 cut(s) 1770, 2176, 2746, 3264, 3436
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 6 cut(s) 859, 1548, 1753, 3042, 3228, 3247
BspQI GCTCTTC 2 cut(s) 1674, 3168
BspT104I TTCGAA 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 3 cut(s) 432, 1442, 2936
BsrDI GCAATG 7 cut(s) 882, 1140, 1500, 1900, 1956, 2645, 2994
BsrFI RCCGGY 1 cut(s) 1117
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 6 cut(s) 392, 516, 1129, 1150, 2158, 3418
BssT1I CCWWGG 5 cut(s) 392, 1129, 1150, 2158, 3418
Bst2UI CCWGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
Bst4CI ACNGT 8 cut(s) 48, 550, 675, 1278, 1574, 2297, 2541, 2683
Bst6I CTCTTC 5 cut(s) 13, 1399, 1674, 2893, 3168
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstBAI YACGTR 3 cut(s) 1295, 2796, 3211
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 6 cut(s) 276, 702, 966, 1138, 1636, 3130
BstDSI CCRYGG 4 cut(s) 392, 516, 2158, 3418
BstENI CCTNNNNNAGG 1 cut(s) 1766
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 2 cut(s) 198, 700
BstMAI GTCTC 9 cut(s) 281, 432, 861, 1442, 1491, 1809, 1929, 2936, 2985
BstMWI GCNNNNNNNGC 5 cut(s) 179, 704, 1142, 2114, 3374
BstNI CCWGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
BstNSI RCATGY 2 cut(s) 1015, 2786
BstSCI CCNGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
BstSFI CTRYAG 5 cut(s) 1766, 2172, 2742, 3260, 3432
BstSNI TACGTA 1 cut(s) 3211
BstUI CGCG 1 cut(s) 198
BstV2I GAAGAC 9 cut(s) 503, 929, 1539, 1740, 2100, 2941, 3033, 3234, 3360
BstX2I RGATCY 5 cut(s) 1540, 1745, 3034, 3220, 3239
BstXI CCANNNNNNTGG 2 cut(s) 393, 1858
BstYI RGATCY 5 cut(s) 1540, 1745, 3034, 3220, 3239
Bsu15I ATCGAT 2 cut(s) 1662, 3156
Bsu36I CCTNAGG 2 cut(s) 1722, 3216
BsuI GTATCC 1 cut(s) 2430
BsuRI GGCC 5 cut(s) 182, 452, 1175, 2790, 3247
BsuTUI ATCGAT 2 cut(s) 1662, 3156
BtgI CCRYGG 4 cut(s) 392, 516, 2158, 3418
BtrI CACGTC 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 1303
BtsIMutI CAGTG 3 cut(s) 619, 671, 1303
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 6 cut(s) 276, 702, 966, 1138, 1636, 3130
CaiI CAGNNNCTG 2 cut(s) 548, 1994
CciI TCATGA 2 cut(s) 1545, 3039
CfoI GCGC 2 cut(s) 198, 700
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 6 cut(s) 268, 450, 896, 944, 1833, 2321
ClaI ATCGAT 2 cut(s) 1662, 3156
CsiI ACCWGGT 1 cut(s) 326
CspAI ACCGGT 1 cut(s) 1117
DriI GACNNNNNGTC 1 cut(s) 3198
EaeI YGGCCR 2 cut(s) 180, 3245
Eam1104I CTCTTC 5 cut(s) 13, 1399, 1674, 2893, 3168
Eam1105I GACNNNNNGTC 1 cut(s) 3198
EarI CTCTTC 5 cut(s) 13, 1399, 1674, 2893, 3168
Ecl136II GAGCTC 1 cut(s) 3377
Eco105I TACGTA 1 cut(s) 3211
Eco130I CCWWGG 5 cut(s) 392, 1129, 1150, 2158, 3418
Eco147I AGGCCT 1 cut(s) 2790
Eco24I GRGCYC 3 cut(s) 1851, 2119, 3379
Eco31I GGTCTC 3 cut(s) 432, 1442, 2936
Eco32I GATATC 2 cut(s) 756, 1453
Eco47I GGWCC 5 cut(s) 268, 896, 944, 1833, 2321
Eco53kI GAGCTC 1 cut(s) 3377
Eco57I CTGAAG 6 cut(s) 141, 486, 2258, 2382, 2631, 2936
Eco81I CCTNAGG 2 cut(s) 1722, 3216
EcoICRI GAGCTC 1 cut(s) 3377
EcoNI CCTNNNNNAGG 1 cut(s) 1766
EcoO109I RGGNCCY 1 cut(s) 896
EcoRII CCWGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
EcoRV GATATC 2 cut(s) 756, 1453
EcoT14I CCWWGG 5 cut(s) 392, 1129, 1150, 2158, 3418
EcoT22I ATGCAT 2 cut(s) 2184, 3444
EcoT38I GRGCYC 3 cut(s) 1851, 2119, 3379
ErhI CCWWGG 5 cut(s) 392, 1129, 1150, 2158, 3418
FalI AAGNNNNNCTT 2 cut(s) 1936, 1968
FaqI GGGAC 4 cut(s) 882, 930, 1743, 3237
FauNDI CATATG 1 cut(s) 956
FbaI TGATCA 5 cut(s) 1510, 1642, 2830, 3004, 3136
FblI GTMKAC 1 cut(s) 2379
FriOI GRGCYC 3 cut(s) 1851, 2119, 3379
FspBI CTAG 3 cut(s) 2192, 2591, 3452
GlaI GCGC 2 cut(s) 197, 699
GsuI CTGGAG 2 cut(s) 1953, 2249
HaeIII GGCC 5 cut(s) 182, 452, 1175, 2790, 3247
HapII CCGG 3 cut(s) 266, 509, 1118
HhaI GCGC 2 cut(s) 198, 700
Hin6I GCGC 2 cut(s) 196, 698
HinP1I GCGC 2 cut(s) 196, 698
HincII GTYRAC 1 cut(s) 1564
HindII GTYRAC 1 cut(s) 1564
HpaII CCGG 3 cut(s) 266, 509, 1118
HphI GGTGA 5 cut(s) 611, 1266, 2062, 2690, 2847
Hpy99I CGWCG 2 cut(s) 43, 893
HpyAV CCTTC 8 cut(s) 277, 361, 799, 1168, 1403, 2606, 2897, 2911
HpyCH4III ACNGT 8 cut(s) 48, 550, 675, 1278, 1574, 2297, 2541, 2683
HpyCH4IV ACGT 7 cut(s) 38, 407, 647, 1239, 1294, 2795, 3210
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 704, 1142, 2114, 3374
HpySE526I ACGT 7 cut(s) 38, 407, 647, 1239, 1294, 2795, 3210
HspAI GCGC 2 cut(s) 196, 698
KflI GGGWCCC 1 cut(s) 896
Ksp22I TGATCA 5 cut(s) 1510, 1642, 2830, 3004, 3136
LguI GCTCTTC 2 cut(s) 1674, 3168
LmnI GCTCC 6 cut(s) 1133, 1412, 2195, 2327, 2615, 3455
MabI ACCWGGT 1 cut(s) 326
MaeI CTAG 3 cut(s) 2192, 2591, 3452
MaeII ACGT 7 cut(s) 38, 407, 647, 1239, 1294, 2795, 3210
MfeI CAATTG 3 cut(s) 528, 1123, 1209
MflI RGATCY 5 cut(s) 1540, 1745, 3034, 3220, 3239
MhlI GDGCHC 3 cut(s) 1851, 2119, 3379
MlsI TGGCCA 2 cut(s) 182, 3247
MluNI TGGCCA 2 cut(s) 182, 3247
MlyI GAGTC 8 cut(s) 292, 1174, 1569, 1699, 1931, 2385, 2730, 3208
Mox20I TGGCCA 2 cut(s) 182, 3247
Mph1103I ATGCAT 2 cut(s) 2184, 3444
MroXI GAANNNNTTC 3 cut(s) 380, 648, 3398
MscI TGGCCA 2 cut(s) 182, 3247
MseI TTAA 1 cut(s) 1037
MslI CAYNNNNRTG 3 cut(s) 391, 2045, 2781
Msp20I TGGCCA 2 cut(s) 182, 3247
MspA1I CMGCKG 4 cut(s) 914, 1991, 2108, 3368
MspI CCGG 3 cut(s) 266, 509, 1118
MspR9I CCNGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
MunI CAATTG 3 cut(s) 528, 1123, 1209
Mva1269I GAATGC 4 cut(s) 115, 382, 767, 2062
MvaI CCWGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 5 cut(s) 179, 704, 1142, 2114, 3374
NcoI CCATGG 3 cut(s) 392, 2158, 3418
NdeI CATATG 1 cut(s) 956
NlaIV GGNNCC 4 cut(s) 897, 898, 946, 1156
NmeAIII GCCGAG 2 cut(s) 187, 1389
NmuCI GTSAC 7 cut(s) 411, 1014, 1284, 1696, 2755, 3190, 3200
NsiI ATGCAT 2 cut(s) 2184, 3444
NspI RCATGY 2 cut(s) 1015, 2786
NspV TTCGAA 1 cut(s) 791
PagI TCATGA 2 cut(s) 1545, 3039
PceI AGGCCT 1 cut(s) 2790
PciI ACATGT 2 cut(s) 1011, 2782
PciSI GCTCTTC 2 cut(s) 1674, 3168
PctI GAATGC 4 cut(s) 115, 382, 767, 2062
PdmI GAANNNNTTC 3 cut(s) 380, 648, 3398
PfeI GAWTC 6 cut(s) 760, 871, 1189, 1683, 2515, 3177
PflFI GACNNNGTC 1 cut(s) 1820
PfoI TCCNGGA 2 cut(s) 1479, 2973
PinAI ACCGGT 1 cut(s) 1117
PleI GAGTC 8 cut(s) 292, 1174, 1568, 1699, 1931, 2384, 2730, 3207
PpsI GAGTC 8 cut(s) 292, 1174, 1568, 1699, 1931, 2384, 2730, 3207
Ppu21I YACGTR 3 cut(s) 1295, 2796, 3211
PpuMI RGGWCCY 1 cut(s) 896
PscI ACATGT 2 cut(s) 1011, 2782
PsiI TTATAA 1 cut(s) 1199
Psp124BI GAGCTC 1 cut(s) 3379
Psp1406I AACGTT 2 cut(s) 647, 1239
Psp5II RGGWCCY 1 cut(s) 896
Psp6I CCWGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
PspGI CCWGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
PspN4I GGNNCC 4 cut(s) 897, 898, 946, 1156
PspPI GGNCC 6 cut(s) 268, 450, 896, 944, 1833, 2321
PspPPI RGGWCCY 1 cut(s) 896
PstI CTGCAG 5 cut(s) 1770, 2176, 2746, 3264, 3436
PstNI CAGNNNCTG 2 cut(s) 548, 1994
PsuI RGATCY 5 cut(s) 1540, 1745, 3034, 3220, 3239
PsyI GACNNNGTC 1 cut(s) 1820
PvuII CAGCTG 4 cut(s) 914, 1991, 2108, 3368
RseI CAYNNNNRTG 3 cut(s) 391, 2045, 2781
SacI GAGCTC 1 cut(s) 3379
SapI GCTCTTC 2 cut(s) 1674, 3168
SaqAI TTAA 1 cut(s) 1037
Sau96I GGNCC 6 cut(s) 268, 450, 896, 944, 1833, 2321
ScaI AGTACT 2 cut(s) 1829, 2567
SchI GAGTC 8 cut(s) 292, 1174, 1569, 1699, 1931, 2385, 2730, 3208
ScrFI CCNGG 8 cut(s) 328, 1177, 1470, 1481, 2319, 2325, 2964, 2975
SduI GDGCHC 3 cut(s) 1851, 2119, 3379
SexAI ACCWGGT 1 cut(s) 326
SfcI CTRYAG 5 cut(s) 1766, 2172, 2742, 3260, 3432
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 5 cut(s) 268, 896, 944, 1833, 2321
SmiMI CAYNNNNRTG 3 cut(s) 391, 2045, 2781
SmlI CTYRAG 4 cut(s) 302, 2424, 2569, 2720
SmoI CTYRAG 4 cut(s) 302, 2424, 2569, 2720
SnaBI TACGTA 1 cut(s) 3211
SseBI AGGCCT 1 cut(s) 2790
SsiI CCGC 4 cut(s) 143, 198, 1102, 3067
SspI AATATT 1 cut(s) 103
SspMI CTAG 3 cut(s) 2192, 2591, 3452
SstI GAGCTC 1 cut(s) 3379
StuI AGGCCT 1 cut(s) 2790
StyD4I CCNGG 8 cut(s) 326, 1175, 1468, 1479, 2317, 2323, 2962, 2973
StyI CCWWGG 5 cut(s) 392, 1129, 1150, 2158, 3418
TaaI ACNGT 8 cut(s) 48, 550, 675, 1278, 1574, 2297, 2541, 2683
TaiI ACGT 7 cut(s) 41, 410, 650, 1242, 1297, 2798, 3213
TatI WGTACW 4 cut(s) 1827, 1925, 2565, 3264
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 6 cut(s) 760, 871, 1189, 1683, 2515, 3177
Tru1I TTAA 1 cut(s) 1037
Tru9I TTAA 1 cut(s) 1037
TscAI CASTG 3 cut(s) 619, 678, 1310
TseFI GTSAC 7 cut(s) 411, 1014, 1284, 1696, 2755, 3190, 3200
Tsp45I GTSAC 7 cut(s) 411, 1014, 1284, 1696, 2755, 3190, 3200
TspGWI ACGGA 2 cut(s) 864, 1063
TspRI CASTG 3 cut(s) 619, 678, 1310
Tth111I GACNNNGTC 1 cut(s) 1820
VpaK11BI GGWCC 5 cut(s) 268, 896, 944, 1833, 2321
XagI CCTNNNNNAGG 1 cut(s) 1766
XapI RAATTY 7 cut(s) 1220, 1596, 1805, 2219, 2521, 2864, 3090
XceI RCATGY 2 cut(s) 1015, 2786
XcmI CCANNNNNNNNNTGG 3 cut(s) 481, 2224, 2708
XmiI GTMKAC 1 cut(s) 2379
XmnI GAANNNNTTC 3 cut(s) 380, 648, 3398
XspI CTAG 3 cut(s) 2192, 2591, 3452
ZrmI AGTACT 2 cut(s) 1829, 2567
Zsp2I ATGCAT 2 cut(s) 2184, 3444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.