MD10G1253600.v1.1

kxDL motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009507
Physical Location & Seq
Forward (+)
34615193 .. 34617742
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1253600.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGAGCAGACGGAGAAAGAAACGATCAGGGAAGCATCGGAGGAGGTGTCAAATGAATTCAAAACACTAATCGATGCTCAGGACTTGGACTCCCTCAAGCAATTGCAGCACCTCATATTGGGGAGATTGCAAGATGCCAATGCAGTTCTGTCACATTTTAATGAGTTTTCAGAGAACTGCTTTGCTGAGGTTTCTGGAGATTTCTCCAGAAACACACGCCTTTTAAAATCTATGAAGACTGATCTTGAATACATATTTCAGAAGTTAAGGAGCATGAAGTCAAGAATTTTGGCTACCTATCCTGATGCATTTCCAGATGACTCGAAACAAGTACTCGACCAAAGACCAGACCTGGAAATGCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KxDL

Protein Analysis

123

Amino Acids

14.14

Weight (kDa)

4.73

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KxDL PF10241 20 - 104 3e-25 Uncharacterized conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 56, 285
AfaI GTAC 1 cut(s) 333
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 2 cut(s) 61, 248
AjnI CCWGG 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 2 cut(s) 56, 285
BbsI GAAGAC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 353
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 353
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 4 cut(s) 44, 64, 124, 295
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 2 cut(s) 190, 216
Bpu10I CCTNAGC 2 cut(s) 78, 186
BpuEI CTTGAG 1 cut(s) 80
Bsa29I ATCGAT 1 cut(s) 72
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 353
BseCI ATCGAT 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 92, 177
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 118
BshVI ATCGAT 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 118
Bsp143I GATC 2 cut(s) 24, 241
BspCNI CTCAG 2 cut(s) 91, 178
BspDI ATCGAT 1 cut(s) 72
BssMI GATC 2 cut(s) 24, 241
Bst2UI CCWGG 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 78, 186
BstKTI GATC 2 cut(s) 27, 244
BstMBI GATC 2 cut(s) 24, 241
BstMWI GCNNNNNNNGC 1 cut(s) 106
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 72
BsuTUI ATCGAT 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 1 cut(s) 332
CviAII CATG 1 cut(s) 274
CviJI RGCY 1 cut(s) 293
CviKI_1 RGCY 1 cut(s) 293
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 2 cut(s) 78, 186
DpnI GATC 2 cut(s) 26, 243
DpnII GATC 2 cut(s) 24, 241
DraI TTTAAA 1 cut(s) 225
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 1 cut(s) 277
FaiI YATR 4 cut(s) 116, 233, 254, 275
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 107
GsuI CTGGAG 2 cut(s) 190, 216
Hin1II CATG 1 cut(s) 277
HinfI GANTC 2 cut(s) 89, 320
Hpy188I TCNGA 3 cut(s) 40, 172, 261
Hpy188III TCNNGA 7 cut(s) 80, 195, 207, 245, 282, 302, 314
HpyCH4V TGCA 4 cut(s) 106, 130, 143, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 106
HpyF3I CTNAG 2 cut(s) 78, 186
Hsp92II CATG 1 cut(s) 277
Kzo9I GATC 2 cut(s) 24, 241
LmnI GCTCC 2 cut(s) 4, 270
LpnPI CCDG 9 cut(s) 13, 65, 180, 220, 315, 327, 338, 360, 365
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 4 cut(s) 44, 64, 124, 295
MaeIII GTNAC 1 cut(s) 150
MalI GATC 2 cut(s) 26, 243
MboI GATC 2 cut(s) 24, 241
MboII GAAGA 1 cut(s) 247
MfeI CAATTG 1 cut(s) 101
MluCI AATT 3 cut(s) 56, 101, 285
MlyI GAGTC 2 cut(s) 83, 314
MnlI CCTC 5 cut(s) 34, 37, 104, 122, 181
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 3 cut(s) 159, 224, 266
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 353
MunI CAATTG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 1 cut(s) 106
NdeII GATC 2 cut(s) 24, 241
NlaIII CATG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 310
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 2 cut(s) 83, 314
PpsI GAGTC 2 cut(s) 83, 314
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 159, 224, 266
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 2 cut(s) 24, 241
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 2 cut(s) 83, 314
ScrFI CCNGG 1 cut(s) 353
SetI ASST 5 cut(s) 48, 114, 192, 299, 354
SfaNI GCATC 4 cut(s) 44, 64, 124, 295
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 56, 101, 285
StyD4I CCNGG 1 cut(s) 351
TaqI TCGA 3 cut(s) 72, 323, 336
TasI AATT 3 cut(s) 56, 101, 285
TatI WGTACW 1 cut(s) 331
Tru1I TTAA 3 cut(s) 159, 224, 266
Tru9I TTAA 3 cut(s) 159, 224, 266
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 3 cut(s) 69, 248, 290
TspGWI ACGGA 1 cut(s) 26
XapI RAATTY 2 cut(s) 56, 285
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.