pycom05g25520

kxDL motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009507
Physical Location & Seq
Forward (+)
26893096 .. 26894762
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25520.1

Sequence Viewer

Length: 369 bp
ATGGAACAGACGGAGAAAGAAACGATCAGGGAAGCATCGGAGGAGATATCGAATGAATTCAAAACACTAATCGATGCTCAGGACTTGGATTCCCTCAAACAATTGCAGCACATCGTATTGGGGAGGTTGCAAGATGGAAATGCAGTCCTGTCACACTTTAATGAGTTTTCAGAGAACTGTTTTGCTGATGTTTCTGCAGATTTCTCCAGAAACACACGCCGTTTAAAGTCTATGAAGACTGATCTTGAATACATATTTCAGAAGTTAAGGAGCATGAAGTCAAGGATTTTGGCTACCTATCCAGATGCATTTCCAGATGACTCGAAACAAGTACTCGATCAAAGGCCGGACCTTGAAATGCCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KxDL

Protein Analysis

123

Amino Acids

14.17

Weight (kDa)

4.81

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KxDL PF10241 20 - 104 2.6e-24 Uncharacterized conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 56
AfaI GTAC 1 cut(s) 333
AgsI TTSAA 3 cut(s) 61, 248, 356
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 349
AvaII GGWCC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 118
BccI CCATC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 349
BmsI GCATC 3 cut(s) 44, 64, 295
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 78
Bsa29I ATCGAT 1 cut(s) 72
BseCI ATCGAT 1 cut(s) 72
BseMII CTCAG 1 cut(s) 92
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 118
BshFI GGCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 72
BsiSI CCGG 1 cut(s) 347
BsnI GGCC 1 cut(s) 346
Bsp143I GATC 3 cut(s) 24, 241, 337
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 91
BspDI ATCGAT 1 cut(s) 72
BspMAI CTGCAG 1 cut(s) 199
BssMI GATC 3 cut(s) 24, 241, 337
Bst4CI ACNGT 1 cut(s) 179
BstDEI CTNAG 1 cut(s) 78
BstKTI GATC 3 cut(s) 27, 244, 340
BstMBI GATC 3 cut(s) 24, 241, 337
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 242
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 349
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 1 cut(s) 332
CviAII CATG 1 cut(s) 274
CviJI RGCY 2 cut(s) 293, 346
CviKI_1 RGCY 2 cut(s) 293, 346
CviQI GTAC 1 cut(s) 332
DdeI CTNAG 1 cut(s) 78
DpnI GATC 3 cut(s) 26, 243, 339
DpnII GATC 3 cut(s) 24, 241, 337
DraI TTTAAA 1 cut(s) 225
Eco32I GATATC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 56
EcoRV GATATC 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 1 cut(s) 277
FaiI YATR 3 cut(s) 233, 254, 275
FatI CATG 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 107
GluI GCNGC 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 347
Hin1II CATG 1 cut(s) 277
HinfI GANTC 2 cut(s) 89, 320
HpaII CCGG 1 cut(s) 347
Hpy188I TCNGA 3 cut(s) 40, 172, 261
Hpy188III TCNNGA 5 cut(s) 80, 207, 245, 302, 314
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 106, 130, 143, 197, 308
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 1 cut(s) 277
Kzo9I GATC 3 cut(s) 24, 241, 337
LmnI GCTCC 1 cut(s) 270
LpnPI CCDG 7 cut(s) 13, 65, 161, 220, 315, 327, 360
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 3 cut(s) 44, 64, 295
MaeIII GTNAC 1 cut(s) 150
MalI GATC 3 cut(s) 26, 243, 339
MboI GATC 3 cut(s) 24, 241, 337
MboII GAAGA 1 cut(s) 247
MfeI CAATTG 1 cut(s) 101
MluCI AATT 2 cut(s) 56, 101
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 3 cut(s) 34, 104, 117
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 3 cut(s) 159, 224, 266
MslI CAYNNNNRTG 1 cut(s) 159
MspI CCGG 1 cut(s) 347
MunI CAATTG 1 cut(s) 101
NdeII GATC 3 cut(s) 24, 241, 337
NlaIII CATG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 310
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 349
PstI CTGCAG 1 cut(s) 199
RsaI GTAC 1 cut(s) 333
RsaNI GTAC 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 3 cut(s) 159, 224, 266
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 3 cut(s) 24, 241, 337
Sau96I GGNCC 1 cut(s) 349
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 1 cut(s) 314
SetI ASST 3 cut(s) 128, 299, 354
SfaNI GCATC 3 cut(s) 44, 64, 295
SfcI CTRYAG 1 cut(s) 195
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 2 cut(s) 56, 101
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 4 cut(s) 50, 72, 323, 336
TasI AATT 2 cut(s) 56, 101
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 159, 224, 266
Tru9I TTAA 3 cut(s) 159, 224, 266
TseFI GTSAC 1 cut(s) 150
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 150
TspDTI ATGAA 3 cut(s) 69, 248, 290
TspGWI ACGGA 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 56
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.