Rorug05G0030200

kxDL motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009507
Physical Location & Seq
Reverse (-)
1913505 .. 1914264
760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0030200.1

Sequence Viewer

Length: 528 bp
ATGTTTTCTCCATGTGAACCAGGACCAGGAGCTGCAAGGATAGAGGGGTATGCTTTGAGAACTGCACAAAAAGATGGAGTCTACATTGGAGAACCAACTACAGTTGATTGGCTATATGTGTATCCTCAAGGAATGGAGAAAATGGTAACATACATAAACGAGAGATACAATAATACACCAATGTTTATTACAGAAAATGGGTATGGTGAGAATTCCACAAATGAAGAACTGTTCCATGATGTCAATAGAGTGGAGTACATGAGAAGTTACTTACAGGCGCTAGCAGATGCAATGAGGCTAACTGAGTTTATGAAAATATGGACAGAACTAAAGGGAGCAGATGTAAGAGGGTACTTCGTGTGGTCATTGCTCGACGATTTCGAGTGGACAAGTGGATATACGGTCAAGTTTGGACTTCACCATGTTGATTATGGCATTCTCAAGAGAACTCCAAGACTCTCTGCAACTTGGTACAGACAATTTATTGCTAATCACACCCTGCAGATTTTAAGTGCCCAGGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KxDL

Protein Analysis

175

Amino Acids

20.43

Weight (kDa)

5.21

Isoelectric Point (pI)

21.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 29 - 165 1.2e-35 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 211
AfaI GTAC 3 cut(s) 257, 353, 473
AfiI CCNNNNNNNGG 1 cut(s) 26
AjnI CCWGG 3 cut(s) 19, 25, 516
AluBI AGCT 1 cut(s) 32
AluI AGCT 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 211
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 2 cut(s) 218, 410
AsuNHI GCTAGC 1 cut(s) 280
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 517
BbvI GCAGC 1 cut(s) 19
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 3 cut(s) 21, 27, 518
BciVI GTATCC 1 cut(s) 132
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 99, 500
BfoI RGCGCY 1 cut(s) 281
BfuI GTATCC 1 cut(s) 132
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 3 cut(s) 21, 27, 518
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 3 cut(s) 21, 27, 518
BmsI GCATC 1 cut(s) 277
BmtI GCTAGC 1 cut(s) 284
BpuEI CTTGAG 2 cut(s) 111, 425
BsaJI CCNNGG 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 327, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse3DI GCAATG 2 cut(s) 297, 365
BseBI CCWGG 3 cut(s) 21, 27, 518
BseDI CCNNGG 1 cut(s) 516
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 2 cut(s) 297, 365
BseMII CTCAG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 26
BsmI GAATGC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 517
BspCNI CTCAG 1 cut(s) 295
BspMAI CTGCAG 1 cut(s) 504
BspOI GCTAGC 1 cut(s) 284
BsrDI GCAATG 2 cut(s) 297, 365
BssECI CCNNGG 1 cut(s) 516
Bst2UI CCWGG 3 cut(s) 21, 27, 518
Bst4CI ACNGT 3 cut(s) 103, 231, 403
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstNI CCWGG 3 cut(s) 21, 27, 518
BstSCI CCNGG 3 cut(s) 19, 25, 516
BstSFI CTRYAG 2 cut(s) 99, 500
BstSLI GKGCMC 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 19
BsuI GTATCC 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 3 cut(s) 256, 352, 472
CviAII CATG 4 cut(s) 12, 236, 259, 422
CviJI RGCY 3 cut(s) 32, 112, 298
CviKI_1 RGCY 3 cut(s) 32, 112, 298
CviQI GTAC 3 cut(s) 256, 352, 472
DdeI CTNAG 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 23
EcoRI GAATTC 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 19, 25, 516
FaeI CATG 4 cut(s) 15, 239, 262, 425
FatI CATG 4 cut(s) 11, 235, 258, 421
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 279
GluI GCNGC 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 281
HhaI GCGC 1 cut(s) 280
Hin1II CATG 4 cut(s) 15, 239, 262, 425
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HinfI GANTC 2 cut(s) 78, 456
HphI GGTGA 2 cut(s) 218, 410
Hpy166II GTNNAC 3 cut(s) 17, 82, 387
Hpy188III TCNNGA 1 cut(s) 442
Hpy8I GTNNAC 3 cut(s) 17, 82, 387
Hpy99I CGWCG 1 cut(s) 377
HpyCH4III ACNGT 3 cut(s) 103, 231, 403
HpyCH4V TGCA 5 cut(s) 35, 65, 290, 464, 502
HpyF3I CTNAG 1 cut(s) 303
Hsp92II CATG 4 cut(s) 15, 239, 262, 425
HspAI GCGC 1 cut(s) 278
LmnI GCTCC 2 cut(s) 29, 335
LpnPI CCDG 7 cut(s) 6, 12, 33, 39, 260, 503, 512
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 2 cut(s) 145, 266
MboII GAAGA 1 cut(s) 236
MhlI GDGCHC 1 cut(s) 517
MluCI AATT 2 cut(s) 211, 479
MlyI GAGTC 2 cut(s) 87, 450
MnlI CCTC 4 cut(s) 37, 135, 288, 341
MseI TTAA 1 cut(s) 509
MspR9I CCNGG 3 cut(s) 21, 27, 518
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 3 cut(s) 21, 27, 518
NheI GCTAGC 1 cut(s) 280
NlaIII CATG 4 cut(s) 15, 239, 262, 425
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 435
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 2 cut(s) 86, 450
PpsI GAGTC 2 cut(s) 86, 450
Psp6I CCWGG 3 cut(s) 19, 25, 516
PspGI CCWGG 3 cut(s) 19, 25, 516
PspPI GGNCC 1 cut(s) 23
PstI CTGCAG 1 cut(s) 504
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 257, 353, 473
RsaNI GTAC 3 cut(s) 256, 352, 472
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 2 cut(s) 87, 450
ScrFI CCNGG 3 cut(s) 21, 27, 518
SduI GDGCHC 1 cut(s) 517
SetI ASST 1 cut(s) 34
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 2 cut(s) 99, 500
SinI GGWCC 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 126, 440
SmoI CTYRAG 2 cut(s) 126, 440
Sse9I AATT 2 cut(s) 211, 479
SspI AATATT 1 cut(s) 524
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 19, 25, 516
TaaI ACNGT 3 cut(s) 103, 231, 403
TaqI TCGA 2 cut(s) 372, 381
TasI AATT 2 cut(s) 211, 479
TatI WGTACW 1 cut(s) 255
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 237, 326
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.