MD05G1301500.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004186
Physical Location & Seq
Reverse (-)
43427807 .. 43431702
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1301500.v1.1.491

Sequence Viewer

Length: 1497 bp
ATGGAATCGCCGGAGAGAGGAAGAAAGAGACATGGGTGCATCGTATTTGGCAGAACAACGCTCAGAAGGATAACAAGAAACATCACTACATTATTGCGTCGATGCCGATTCCATCCCTTTGATTCAACCGAGAAATTAGGATCCCTCTCTCTCTCCTTCTCTCTCTCTCTCTCTATATATATATACACATACATGGCGTCCGCTTCTCTGATTCACGCGCGCTTTGGGTACTTACCGAGTTTCAGCGGCAATCGGCGGAACGACTCCCAGGGACCTCTCGGTTCTTCGTCCTCCTCCTCCTCTTCGTCTTTCAGCTTCGGTTCTGGCATATATAGTAGTTGGAGAAATCAAGGTCTTAGAGCTCAAGCTATGACTAGTCAGGGAAATTTCGGATCCTCCAGGGGTATTGCAAATGCAGAGGAGAAACCCGATCATCTTCTCGTCCTTGTTCATGGCATCATGGCAAGCCCAAGTGACTGGACATACGTGGAAGCGGAGTTAAAAAGGCGTCTAGGAAGAAATTTCTTAATCTATGCAAGTTCATGTAACACGTACACCAAAACGTTTTCTGGTATTGATGGAGCCGGGAAACGATTAGCAGATGAAGTTATGCAACTTGTACAAAAGACAGAGAGCTTGAAGAAGATCTCATTTTTAGCCCATTCTCTTGGGGGCTTATTTGCAAGATATGCAATTTCTGTCCTTTACACACCCAATGATAGTGCCAGTTCAACTACTGCAAATTCACAAACGGCGCACTCTTTGAGAGGAGGAAAGATTGCTGGTTTAGAGCCTATCAATTTTGTAACATTGGCAACTCCACATCTTGGGGTGCGAGGGAAAAATCAGCTTCCATTTTTATTGGGAGTACCTTTTTTAGAAAAGCTGGCTGCGCCAATTGCTCCTATTATTGTTGGTCGAACTGGTAGTCAGCTGTTTCTCACTGATGGTAAGCCCGAAAGACCACCACTTCTTTTGAGAATGGCATCCGATTGCGAAGATGGAAAATTTATATCTTCTTTGGGCTCCTTCAAATCTCGGATTATTTATGCCAATGTATCATATGATCATATGGTTGGCTGGCGTACATCATCCATTAGGAGGGAGACGGAAATATTTAAGCCTCCTCGCCGGTCTTTGGACGGGTACAAGCATGTTGTAGACGTGGAGTACTGTCCTCCAGTTTCCTCTGATGGTCCCCATTTTCCTCCCAAAGCAGCTAAAGCAAAAGAAGCAGCACAAAGCGAACCTAACATACAGAACACATTAGAATATCACGAAATTGTTGAAGATGAAATGATACGGGGATTACAGCAGTTAGGATGGAGAAAAGTTGATGTCAGCTTTCACTCGGCGTTTTTGCCCTTCTTTGCCCACAATAACATCCATGTGAAGAATGAATGGCTTCACAATGCTGGCGTCGGGGTGGTTGCTCACGTTGCCGACAGCCTGAAGCAACAAGAATCCTCCTCCTCATTCATCACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

499

Amino Acids

54.91

Weight (kDa)

9.6

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 141 - 363 2e-66 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 827
AccI GTMKAC 1 cut(s) 1161
AccII CGCG 2 cut(s) 218, 220
AciI CCGC 4 cut(s) 201, 246, 256, 494
AclI AACGTT 1 cut(s) 563
AclWI GGATC 4 cut(s) 135, 148, 387, 400
AcsI RAATTY 4 cut(s) 385, 520, 742, 1007
AcuI CTGAAG 1 cut(s) 1472
AcyI GRCGYC 3 cut(s) 197, 508, 1419
AfaI GTAC 7 cut(s) 230, 554, 621, 870, 1087, 1148, 1172
AfiI CCNNNNNNNGG 4 cut(s) 17, 827, 1101, 1138
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 5 cut(s) 126, 640, 732, 1033, 1289
AhlI ACTAGT 1 cut(s) 374
AjiI CACGTC 1 cut(s) 1165
AjnI CCWGG 2 cut(s) 267, 398
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 2 cut(s) 22, 1100
AlwI GGATC 4 cut(s) 135, 148, 387, 400
ApeKI GCWGC 3 cut(s) 890, 1217, 1235
ApoI RAATTY 4 cut(s) 385, 520, 742, 1007
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 4 cut(s) 220, 222, 757, 895
AspS9I GGNCC 2 cut(s) 272, 1196
AsuC2I CCSGG 1 cut(s) 586
AvaII GGWCC 2 cut(s) 272, 1196
BamHI GGATCC 2 cut(s) 140, 392
BanII GRGCYC 2 cut(s) 364, 1028
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 877, 1229, 1247
BccI CCATC 6 cut(s) 120, 572, 941, 995, 1187, 1317
BceAI ACGGC 1 cut(s) 768
BciT130I CCWGG 2 cut(s) 269, 400
BclI TGATCA 1 cut(s) 1066
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 22, 1100
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 2 cut(s) 375, 512
BglII AGATCT 1 cut(s) 645
BisI GCNGC 4 cut(s) 247, 891, 1218, 1236
BlsI GCNGC 4 cut(s) 248, 892, 1219, 1237
BmcAI AGTACT 1 cut(s) 1172
Bme1390I CCNGG 3 cut(s) 269, 400, 586
Bme18I GGWCC 2 cut(s) 272, 1196
BmgBI CACGTC 1 cut(s) 1165
BmgT120I GGNCC 2 cut(s) 272, 1196
BmiI GGNNCC 6 cut(s) 142, 273, 394, 583, 1027, 1198
BmrFI CCNGG 3 cut(s) 269, 400, 586
BmsI GCATC 4 cut(s) 48, 92, 465, 995
BpmI CTGGAG 2 cut(s) 382, 1164
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 586
BsaAI YACGTR 2 cut(s) 487, 552
BsaHI GRCGYC 3 cut(s) 197, 508, 1419
BsaJI CCNNGG 3 cut(s) 267, 268, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 827, 1101, 1138
Bse118I RCCGGY 1 cut(s) 1131
Bse1I ACTGG 4 cut(s) 482, 726, 928, 1181
BseBI CCWGG 2 cut(s) 269, 400
BseDI CCNNGG 3 cut(s) 267, 268, 399
BseGI GGATG 5 cut(s) 112, 986, 1091, 1328, 1383
BseLI CCNNNNNNNGG 4 cut(s) 17, 827, 1101, 1138
BseMII CTCAG 1 cut(s) 76
BseNI ACTGG 4 cut(s) 482, 726, 928, 1181
BsePI GCGCGC 1 cut(s) 218
BseRI GAGGAG 8 cut(s) 283, 286, 289, 434, 783, 1116, 1459, 1462
BseXI GCAGC 3 cut(s) 877, 1229, 1247
Bsh1236I CGCG 2 cut(s) 218, 220
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 3 cut(s) 11, 585, 1132
BslFI GGGAC 2 cut(s) 285, 1182
BslI CCNNNNNNNGG 4 cut(s) 17, 827, 1101, 1138
BsmAI GTCTC 2 cut(s) 22, 1100
BsmBI CGTCTC 1 cut(s) 1100
BsmFI GGGAC 2 cut(s) 285, 1182
Bsp1286I GDGCHC 2 cut(s) 364, 1028
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 5 cut(s) 140, 392, 430, 645, 1066
BspACI CCGC 4 cut(s) 201, 246, 256, 494
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 2 cut(s) 218, 220
BspLI GGNNCC 6 cut(s) 142, 273, 394, 583, 1027, 1198
BspPI GGATC 4 cut(s) 135, 148, 387, 400
BsrFI RCCGGY 1 cut(s) 1131
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 4 cut(s) 482, 726, 928, 1181
BssAI RCCGGY 1 cut(s) 1131
BssECI CCNNGG 3 cut(s) 267, 268, 399
BssHII GCGCGC 1 cut(s) 218
BssMI GATC 5 cut(s) 140, 392, 430, 645, 1066
BssNI GRCGYC 3 cut(s) 197, 508, 1419
Bst2UI CCWGG 2 cut(s) 269, 400
Bst4CI ACNGT 1 cut(s) 1175
Bst6I CTCTTC 1 cut(s) 307
BstACI GRCGYC 3 cut(s) 197, 508, 1419
BstAPI GCANNNNNTGC 1 cut(s) 689
BstAUI TGTACA 1 cut(s) 619
BstBAI YACGTR 2 cut(s) 487, 552
BstC8I GCNNGC 6 cut(s) 220, 466, 888, 1082, 1417, 1489
BstDEI CTNAG 2 cut(s) 62, 356
BstF5I GGATG 5 cut(s) 112, 986, 1091, 1328, 1383
BstFNI CGCG 2 cut(s) 218, 220
BstHHI GCGC 4 cut(s) 220, 222, 757, 895
BstKTI GATC 5 cut(s) 143, 395, 433, 648, 1069
BstMAI GTCTC 2 cut(s) 22, 1100
BstMBI GATC 5 cut(s) 140, 392, 430, 645, 1066
BstMWI GCNNNNNNNGC 6 cut(s) 689, 892, 899, 1223, 1232, 1439
BstNI CCWGG 2 cut(s) 269, 400
BstNSI RCATGY 1 cut(s) 1157
BstSCI CCNGG 3 cut(s) 267, 398, 584
BstUI CGCG 2 cut(s) 218, 220
BstV1I GCAGC 3 cut(s) 877, 1229, 1247
BstX2I RGATCY 3 cut(s) 140, 392, 645
BstXI CCANNNNNNTGG 2 cut(s) 477, 668
BstYI RGATCY 3 cut(s) 140, 392, 645
BtrI CACGTC 1 cut(s) 1165
BtsCI GGATG 5 cut(s) 112, 986, 1091, 1328, 1383
BtsI GCAGTG 1 cut(s) 1482
BtsIMutI CAGTG 2 cut(s) 942, 1482
Cac8I GCNNGC 6 cut(s) 220, 466, 888, 1082, 1417, 1489
CfoI GCGC 4 cut(s) 220, 222, 757, 895
Cfr10I RCCGGY 1 cut(s) 1131
Cfr13I GGNCC 2 cut(s) 272, 1196
CseI GACGC 4 cut(s) 86, 186, 497, 1408
Csp6I GTAC 7 cut(s) 229, 553, 620, 869, 1086, 1147, 1171
CspCI CAANNNNNGTGG 2 cut(s) 957, 992
CviAII CATG 7 cut(s) 32, 193, 452, 460, 543, 1154, 1388
CviQI GTAC 7 cut(s) 229, 553, 620, 869, 1086, 1147, 1171
DdeI CTNAG 2 cut(s) 62, 356
DpnI GATC 5 cut(s) 142, 394, 432, 647, 1068
DpnII GATC 5 cut(s) 140, 392, 430, 645, 1066
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
EciI GGCGGA 1 cut(s) 271
Ecl136II GAGCTC 1 cut(s) 362
Eco24I GRGCYC 2 cut(s) 364, 1028
Eco47I GGWCC 2 cut(s) 272, 1196
Eco53kI GAGCTC 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 1472
EcoICRI GAGCTC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 267, 398
EcoT38I GRGCYC 2 cut(s) 364, 1028
Esp3I CGTCTC 1 cut(s) 1100
FaeI CATG 7 cut(s) 35, 196, 455, 463, 546, 1157, 1391
FaqI GGGAC 2 cut(s) 285, 1182
FatI CATG 7 cut(s) 31, 192, 451, 459, 542, 1153, 1387
FauNDI CATATG 2 cut(s) 1063, 1071
FbaI TGATCA 1 cut(s) 1066
FblI GTMKAC 1 cut(s) 1161
Fnu4HI GCNGC 4 cut(s) 247, 891, 1218, 1236
FokI GGATG 5 cut(s) 99, 973, 1078, 1335, 1370
FriOI GRGCYC 2 cut(s) 364, 1028
Fsp4HI GCNGC 4 cut(s) 247, 891, 1218, 1236
FspBI CTAG 2 cut(s) 375, 512
GlaI GCGC 4 cut(s) 219, 221, 756, 894
GluI GCNGC 4 cut(s) 247, 891, 1218, 1236
GsuI CTGGAG 2 cut(s) 382, 1164
HapII CCGG 3 cut(s) 11, 585, 1132
HgaI GACGC 4 cut(s) 86, 186, 497, 1408
HhaI GCGC 4 cut(s) 220, 222, 757, 895
Hin1I GRCGYC 3 cut(s) 197, 508, 1419
Hin1II CATG 7 cut(s) 35, 196, 455, 463, 546, 1157, 1391
Hin6I GCGC 4 cut(s) 218, 220, 755, 893
HinP1I GCGC 4 cut(s) 218, 220, 755, 893
HinfI GANTC 6 cut(s) 5, 108, 122, 211, 263, 1463
HpaII CCGG 3 cut(s) 11, 585, 1132
Hpy166II GTNNAC 2 cut(s) 555, 1162
Hpy188I TCNGA 6 cut(s) 65, 210, 392, 991, 1041, 1192
Hpy188III TCNNGA 1 cut(s) 1277
Hpy8I GTNNAC 2 cut(s) 555, 1162
Hpy99I CGWCG 2 cut(s) 102, 1424
HpyAV CCTTC 4 cut(s) 60, 166, 1039, 1375
HpyCH4III ACNGT 1 cut(s) 1175
HpyCH4IV ACGT 5 cut(s) 486, 551, 563, 1164, 1437
HpyCH4V TGCA 8 cut(s) 39, 410, 416, 536, 613, 683, 692, 740
HpyF10VI GCNNNNNNNGC 6 cut(s) 689, 892, 899, 1223, 1232, 1439
HpyF3I CTNAG 2 cut(s) 62, 356
HpySE526I ACGT 5 cut(s) 486, 551, 563, 1164, 1437
Hsp92I GRCGYC 3 cut(s) 197, 508, 1419
Hsp92II CATG 7 cut(s) 35, 196, 455, 463, 546, 1157, 1391
HspAI GCGC 4 cut(s) 218, 220, 755, 893
Ksp22I TGATCA 1 cut(s) 1066
Kzo9I GATC 5 cut(s) 140, 392, 430, 645, 1066
LmnI GCTCC 3 cut(s) 581, 907, 1031
Lsp1109I GCAGC 3 cut(s) 877, 1229, 1247
LweI GCATC 4 cut(s) 48, 92, 465, 995
MaeI CTAG 2 cut(s) 375, 512
MaeII ACGT 5 cut(s) 486, 551, 563, 1164, 1437
MaeIII GTNAC 3 cut(s) 473, 545, 805
MalI GATC 5 cut(s) 142, 394, 432, 647, 1068
MboI GATC 5 cut(s) 140, 392, 430, 645, 1066
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 3 cut(s) 140, 392, 645
MhlI GDGCHC 2 cut(s) 364, 1028
MluCI AATT 9 cut(s) 134, 385, 520, 693, 742, 799, 897, 1007, 1281
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 1404
MseI TTAA 3 cut(s) 500, 527, 1119
MslI CAYNNNNRTG 2 cut(s) 191, 1388
MspA1I CMGCKG 2 cut(s) 246, 934
MspI CCGG 3 cut(s) 11, 585, 1132
MspR9I CCNGG 3 cut(s) 269, 400, 586
MunI CAATTG 1 cut(s) 897
MvaI CCWGG 2 cut(s) 269, 400
MvnI CGCG 2 cut(s) 218, 220
MwoI GCNNNNNNNGC 6 cut(s) 689, 892, 899, 1223, 1232, 1439
NciI CCSGG 1 cut(s) 586
NdeI CATATG 2 cut(s) 1063, 1071
NdeII GATC 5 cut(s) 140, 392, 430, 645, 1066
NlaIII CATG 7 cut(s) 35, 196, 455, 463, 546, 1157, 1391
NlaIV GGNNCC 6 cut(s) 142, 273, 394, 583, 1027, 1198
NmeAIII GCCGAG 1 cut(s) 1331
NmuCI GTSAC 1 cut(s) 473
NspI RCATGY 1 cut(s) 1157
PasI CCCWGGG 1 cut(s) 268
PauI GCGCGC 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 1404
PfeI GAWTC 5 cut(s) 5, 108, 122, 211, 1463
PflMI CCANNNNNTGG 1 cut(s) 827
PkrI GCNGC 4 cut(s) 248, 892, 1219, 1237
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 2 cut(s) 487, 552
PpuMI RGGWCCY 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 364
Psp1406I AACGTT 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 267, 398
PspGI CCWGG 2 cut(s) 267, 398
PspN4I GGNNCC 6 cut(s) 142, 273, 394, 583, 1027, 1198
PspPI GGNCC 2 cut(s) 272, 1196
PspPPI RGGWCCY 1 cut(s) 272
PsrI GAACNNNNNNTAC 2 cut(s) 1239, 1271
PsuI RGATCY 3 cut(s) 140, 392, 645
PteI GCGCGC 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 934
RsaI GTAC 7 cut(s) 230, 554, 621, 870, 1087, 1148, 1172
RsaNI GTAC 7 cut(s) 229, 553, 620, 869, 1086, 1147, 1171
RseI CAYNNNNRTG 2 cut(s) 191, 1388
SacI GAGCTC 1 cut(s) 364
SaqAI TTAA 3 cut(s) 500, 527, 1119
SatI GCNGC 4 cut(s) 247, 891, 1218, 1236
Sau3AI GATC 5 cut(s) 140, 392, 430, 645, 1066
Sau96I GGNCC 2 cut(s) 272, 1196
ScaI AGTACT 1 cut(s) 1172
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 269, 400, 586
SduI GDGCHC 2 cut(s) 364, 1028
SfaNI GCATC 4 cut(s) 48, 92, 465, 995
SinI GGWCC 2 cut(s) 272, 1196
SmiMI CAYNNNNRTG 2 cut(s) 191, 1388
SmlI CTYRAG 1 cut(s) 363
SmoI CTYRAG 1 cut(s) 363
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 9 cut(s) 134, 385, 520, 693, 742, 799, 897, 1007, 1281
SsiI CCGC 4 cut(s) 201, 246, 256, 494
SspI AATATT 1 cut(s) 1116
SspMI CTAG 2 cut(s) 375, 512
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 3 cut(s) 267, 398, 584
TaaI ACNGT 1 cut(s) 1175
TaiI ACGT 5 cut(s) 489, 554, 566, 1167, 1440
TaqI TCGA 2 cut(s) 100, 919
TasI AATT 9 cut(s) 134, 385, 520, 693, 742, 799, 897, 1007, 1281
TatI WGTACW 2 cut(s) 619, 1170
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 5 cut(s) 5, 108, 122, 211, 1463
Tru1I TTAA 3 cut(s) 500, 527, 1119
Tru9I TTAA 3 cut(s) 500, 527, 1119
TscAI CASTG 2 cut(s) 949, 1489
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 3 cut(s) 890, 1217, 1235
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 6 cut(s) 440, 531, 618, 1308, 1413, 1468
TspGWI ACGGA 1 cut(s) 1124
TspRI CASTG 2 cut(s) 949, 1489
Van91I CCANNNNNTGG 1 cut(s) 827
VpaK11BI GGWCC 2 cut(s) 272, 1196
XapI RAATTY 4 cut(s) 385, 520, 742, 1007
XceI RCATGY 1 cut(s) 1157
XmiI GTMKAC 1 cut(s) 1161
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 2 cut(s) 375, 512
ZrmI AGTACT 1 cut(s) 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.