Rh5AG083800

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004186
Physical Location & Seq
Forward (+)
7207738 .. 7210963
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG083800.1

Sequence Viewer

Length: 1329 bp
ATGGCCTCCGCTCCTCTAATCCACGCGCGCTGTGGTTACTCGCCCAGATTCAGTAGCAGCAGTAATCGACGGAATGGAGGAGGAGGAGCTCTAGGTTCGTCGCCGTCGTCGTCGTCTTCGTCGTCGTGCTGCTCTTCTTCGTATTTGGCCTTCTCTTCTGGGGCGTATAATTGCTGGAGGAACCAAGGACTCAGAGCTCACGCTATGACCAGTCAGGGGAGTTTTGCTTCTTCCTCCAGAGGTGTTGCGAGTGCGGGGAAGAAGCCCGATCATCTTCTCGTACTTGTTCATGGCATATTGGCTAGCCCAAGTGATTGGACATACGTGGAAGCAGAATTACGAAAGCGTCTAGGCAGAAGCTTCTTAATCTATGCAAGTTCATGCAACACGTACACAAAAACTTTTGCCGGGATTGATGGAGCTGGAAAGAGACTGGCAGATGAAGTCATGCAAGTTGTGCAAAAGACAGATAGCCTCAAGAAAATCTCATTCTTAGCTCATTCTCTAGGGGGCTTGTTTGCAAGATACGCAATATCTGTTCTTTACAGACCCAGTGATATTCCCAATTCACCTCTTGTAAATTCAAAAGCAACACACTCTTTGAGACGAGGAAAGATTGCCGGTTTGGAGCCAATCAATTTTGTGACATTGGCAACCCCACATCTTGGGGTGAGGGGGAAAAAGCAGCTTCCATTTCTTTTAGGCGTACCTTTGTTAGAAAAGCTGGCTGCGCCAATTGCTCCTATTGTTGTTGGTCGAACTGGTAGTCAGCTGTTCCTCACTGATGGTAAACCTGAAAAACCACCGCTTCTTTTGAGAATGGCATCTGATTGTGAAGATGGAAATTTTGTATCTGCATTGGGATCGTTTAGATCTCGGATTCTTTATGCTAATGTATCTTATGATCATATGGTTGGTTGGCGTACATCATCCATAAGGAGAGAGACAGAAATTTTTAAGCCTCCTCGCCGGTCTTTGGTTGGCTACAAGCATGTTGTAGATGTGGAGTATTGTCCCCCAGTTTCCTCTGACGGCCCCCATTTTCCTCCTGAAGCAGCTAAGGCGAAGGAGGCAGCCCAAAGTAAACCCAACCAACAGAACACCTTGGAATATCATGAAATTGTTGAAGATGAAATGATACGAGGCTTACAACAGTTGGGATGGAAAAAAGTTGATGTCAGCTTTCATTCTGCACCTTGGCCATTCTTTGCCCACAACAACATTCATGTAAAAAATGAATGGCTTCACAATGCTGGTGCTGGAGTGGTTGCACACGTTGCTGACACTCTAAAGCAACAAGAATCCTCCACATTGACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

442

Amino Acids

47.93

Weight (kDa)

9.59

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 87 - 309 1.2e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 665
AccBSI CCGCTC 1 cut(s) 11
AccII CGCG 2 cut(s) 26, 28
AciI CCGC 3 cut(s) 9, 254, 806
AclWI GGATC 1 cut(s) 871
AcoI YGGCCR 1 cut(s) 1199
AcsI RAATTY 3 cut(s) 580, 844, 951
AcuI CTGAAG 1 cut(s) 1071
AfaI GTAC 4 cut(s) 282, 392, 708, 925
AfiI CCNNNNNNNGG 3 cut(s) 216, 665, 976
AflIII ACRYGT 2 cut(s) 387, 1273
AgsI TTSAA 2 cut(s) 585, 1127
Alw21I GWGCWC 2 cut(s) 91, 199
Alw26I GTCTC 3 cut(s) 424, 598, 938
AlwI GGATC 1 cut(s) 871
AoxI GGCC 4 cut(s) 3, 147, 1033, 1199
ApeKI GCWGC 6 cut(s) 57, 129, 685, 728, 1055, 1073
ApoI RAATTY 3 cut(s) 580, 844, 951
ArsI GACNNNNNNTTYG 2 cut(s) 751, 783
Asp700I GAANNNNTTC 1 cut(s) 1242
AspLEI GCGC 3 cut(s) 28, 30, 733
AspS9I GGNCC 1 cut(s) 1034
AsuC2I CCSGG 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 561, 682
AsuNHI GCTAGC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 1201
BanII GRGCYC 2 cut(s) 91, 199
BarI GAAGNNNNNNTAC 4 cut(s) 134, 166, 321, 353
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 91, 199
BbvI GCAGC 6 cut(s) 69, 116, 697, 715, 1067, 1085
BccI CCATC 4 cut(s) 410, 779, 833, 1155
BceAI ACGGC 2 cut(s) 88, 1048
BcgI CGANNNNNNTGC 2 cut(s) 846, 880
BclI TGATCA 1 cut(s) 904
BcnI CCSGG 1 cut(s) 409
BcoDI GTCTC 3 cut(s) 424, 598, 938
BfaI CTAG 4 cut(s) 92, 303, 350, 506
BglII AGATCT 1 cut(s) 872
BisI GCNGC 6 cut(s) 58, 130, 686, 729, 1056, 1074
BlsI GCNGC 6 cut(s) 59, 131, 687, 730, 1057, 1075
Bme1390I CCNGG 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 1034
BmiI GGNNCC 3 cut(s) 182, 630, 1036
BmrFI CCNGG 1 cut(s) 409
BmrI ACTGGG 2 cut(s) 546, 1013
BmsI GCATC 1 cut(s) 833
BmtI GCTAGC 1 cut(s) 306
BmuI ACTGGG 2 cut(s) 546, 1013
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 3 cut(s) 196, 220, 1281
Bpu10I CCTNAGC 1 cut(s) 1059
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 409
BsaAI YACGTR 2 cut(s) 325, 390
BsaJI CCNNGG 3 cut(s) 184, 1104, 1196
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 665, 976
Bse118I RCCGGY 2 cut(s) 620, 969
Bse1I ACTGG 5 cut(s) 210, 438, 552, 766, 1019
BseDI CCNNGG 3 cut(s) 184, 1104, 1196
BseGI GGATG 2 cut(s) 929, 1166
BseLI CCNNNNNNNGG 3 cut(s) 216, 665, 976
BseMII CTCAG 1 cut(s) 205
BseNI ACTGG 5 cut(s) 210, 438, 552, 766, 1019
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 4 cut(s) 93, 96, 99, 954
BseXI GCAGC 6 cut(s) 69, 116, 697, 715, 1067, 1085
BsgI GTGCAG 1 cut(s) 1176
Bsh1236I CGCG 2 cut(s) 26, 28
BshFI GGCC 4 cut(s) 5, 149, 1035, 1201
BsiHKAI GWGCWC 2 cut(s) 91, 199
BsiSI CCGG 3 cut(s) 408, 621, 970
BslFI GGGAC 1 cut(s) 999
BslI CCNNNNNNNGG 3 cut(s) 216, 665, 976
BsmAI GTCTC 3 cut(s) 424, 598, 938
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 999
BsnI GGCC 4 cut(s) 5, 149, 1035, 1201
Bsp1286I GDGCHC 2 cut(s) 91, 199
Bsp143I GATC 4 cut(s) 268, 863, 872, 904
BspACI CCGC 3 cut(s) 9, 254, 806
BspANI GGCC 4 cut(s) 5, 149, 1035, 1201
BspCNI CTCAG 1 cut(s) 204
BspFNI CGCG 2 cut(s) 26, 28
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 3 cut(s) 182, 630, 1036
BspOI GCTAGC 1 cut(s) 306
BspPI GGATC 1 cut(s) 871
BspQI GCTCTTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 11
BsrFI RCCGGY 2 cut(s) 620, 969
BsrI ACTGG 5 cut(s) 210, 438, 552, 766, 1019
BssAI RCCGGY 2 cut(s) 620, 969
BssECI CCNNGG 3 cut(s) 184, 1104, 1196
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 4 cut(s) 268, 863, 872, 904
BssT1I CCWWGG 3 cut(s) 184, 1104, 1196
Bst4CI ACNGT 1 cut(s) 1155
Bst6I CTCTTC 2 cut(s) 139, 160
BstAPI GCANNNNNTGC 2 cut(s) 457, 1277
BstBAI YACGTR 2 cut(s) 325, 390
BstC8I GCNNGC 4 cut(s) 28, 304, 726, 1321
BstDEI CTNAG 3 cut(s) 191, 493, 1059
BstF5I GGATG 2 cut(s) 929, 1166
BstFNI CGCG 2 cut(s) 26, 28
BstHHI GCGC 3 cut(s) 28, 30, 733
BstKTI GATC 4 cut(s) 271, 866, 875, 907
BstMAI GTCTC 3 cut(s) 424, 598, 938
BstMBI GATC 4 cut(s) 268, 863, 872, 904
BstMWI GCNNNNNNNGC 7 cut(s) 457, 527, 730, 737, 1061, 1070, 1277
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 1 cut(s) 407
BstUI CGCG 2 cut(s) 26, 28
BstV1I GCAGC 6 cut(s) 69, 116, 697, 715, 1067, 1085
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 872
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 1 cut(s) 872
BsuRI GGCC 4 cut(s) 5, 149, 1035, 1201
BtsCI GGATG 2 cut(s) 929, 1166
BtsIMutI CAGTG 2 cut(s) 559, 780
Cac8I GCNNGC 4 cut(s) 28, 304, 726, 1321
CciI TCATGA 1 cut(s) 1114
CfoI GCGC 3 cut(s) 28, 30, 733
Cfr10I RCCGGY 2 cut(s) 620, 969
Cfr13I GGNCC 1 cut(s) 1034
CseI GACGC 1 cut(s) 335
Csp6I GTAC 4 cut(s) 281, 391, 707, 924
CviAII CATG 6 cut(s) 290, 381, 448, 992, 1115, 1226
CviQI GTAC 4 cut(s) 281, 391, 707, 924
DdeI CTNAG 3 cut(s) 191, 493, 1059
DpnI GATC 4 cut(s) 270, 865, 874, 906
DpnII GATC 4 cut(s) 268, 863, 872, 904
EaeI YGGCCR 1 cut(s) 1199
Eam1104I CTCTTC 2 cut(s) 139, 160
EarI CTCTTC 2 cut(s) 139, 160
Ecl136II GAGCTC 2 cut(s) 89, 197
Eco130I CCWWGG 3 cut(s) 184, 1104, 1196
Eco24I GRGCYC 2 cut(s) 91, 199
Eco53kI GAGCTC 2 cut(s) 89, 197
Eco57I CTGAAG 1 cut(s) 1071
EcoICRI GAGCTC 2 cut(s) 89, 197
EcoT14I CCWWGG 3 cut(s) 184, 1104, 1196
EcoT38I GRGCYC 2 cut(s) 91, 199
ErhI CCWWGG 3 cut(s) 184, 1104, 1196
Esp3I CGTCTC 1 cut(s) 598
FaeI CATG 6 cut(s) 293, 384, 451, 995, 1118, 1229
FaqI GGGAC 1 cut(s) 999
FatI CATG 6 cut(s) 289, 380, 447, 991, 1114, 1225
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 1 cut(s) 904
Fnu4HI GCNGC 6 cut(s) 58, 130, 686, 729, 1056, 1074
FokI GGATG 2 cut(s) 916, 1173
FriOI GRGCYC 2 cut(s) 91, 199
Fsp4HI GCNGC 6 cut(s) 58, 130, 686, 729, 1056, 1074
FspBI CTAG 4 cut(s) 92, 303, 350, 506
GlaI GCGC 3 cut(s) 27, 29, 732
GluI GCNGC 6 cut(s) 58, 130, 686, 729, 1056, 1074
GsuI CTGGAG 3 cut(s) 196, 220, 1281
HaeIII GGCC 4 cut(s) 5, 149, 1035, 1201
HapII CCGG 3 cut(s) 408, 621, 970
HgaI GACGC 1 cut(s) 335
HhaI GCGC 3 cut(s) 28, 30, 733
Hin1II CATG 6 cut(s) 293, 384, 451, 995, 1118, 1229
Hin6I GCGC 3 cut(s) 26, 28, 731
HinP1I GCGC 3 cut(s) 26, 28, 731
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 4 cut(s) 48, 189, 880, 1301
HpaII CCGG 3 cut(s) 408, 621, 970
HphI GGTGA 2 cut(s) 561, 682
Hpy166II GTNNAC 3 cut(s) 393, 791, 1085
Hpy188I TCNGA 4 cut(s) 194, 829, 879, 1030
Hpy188III TCNNGA 4 cut(s) 237, 478, 1049, 1115
Hpy8I GTNNAC 3 cut(s) 393, 791, 1085
Hpy99I CGWCG 7 cut(s) 72, 103, 109, 112, 115, 124, 127
HpyAV CCTTC 2 cut(s) 160, 1060
HpyCH4III ACNGT 1 cut(s) 1155
HpyCH4IV ACGT 3 cut(s) 324, 389, 1275
HpyCH4V TGCA 8 cut(s) 374, 384, 451, 460, 521, 857, 1193, 1271
HpyF10VI GCNNNNNNNGC 7 cut(s) 457, 527, 730, 737, 1061, 1070, 1277
HpyF3I CTNAG 3 cut(s) 191, 493, 1059
HpySE526I ACGT 3 cut(s) 324, 389, 1275
Hsp92II CATG 6 cut(s) 293, 384, 451, 995, 1118, 1229
HspAI GCGC 3 cut(s) 26, 28, 731
Ksp22I TGATCA 1 cut(s) 904
Kzo9I GATC 4 cut(s) 268, 863, 872, 904
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 5 cut(s) 16, 86, 419, 628, 745
Lsp1109I GCAGC 6 cut(s) 69, 116, 697, 715, 1067, 1085
LweI GCATC 1 cut(s) 833
MaeI CTAG 4 cut(s) 92, 303, 350, 506
MaeII ACGT 3 cut(s) 324, 389, 1275
MaeIII GTNAC 2 cut(s) 35, 643
MalI GATC 4 cut(s) 270, 865, 874, 906
MbiI CCGCTC 1 cut(s) 11
MboI GATC 4 cut(s) 268, 863, 872, 904
MboII GAAGA 9 cut(s) 108, 126, 129, 147, 222, 266, 271, 848, 1139
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 872
MhlI GDGCHC 2 cut(s) 91, 199
MlsI TGGCCA 1 cut(s) 1201
MluCI AATT 9 cut(s) 169, 335, 565, 580, 637, 735, 844, 951, 1119
MluNI TGGCCA 1 cut(s) 1201
MlyI GAGTC 1 cut(s) 183
Mox20I TGGCCA 1 cut(s) 1201
MroXI GAANNNNTTC 1 cut(s) 1242
MscI TGGCCA 1 cut(s) 1201
MseI TTAA 2 cut(s) 365, 957
Msp20I TGGCCA 1 cut(s) 1201
MspA1I CMGCKG 1 cut(s) 772
MspI CCGG 3 cut(s) 408, 621, 970
MspR9I CCNGG 1 cut(s) 409
MunI CAATTG 1 cut(s) 735
MvnI CGCG 2 cut(s) 26, 28
MwoI GCNNNNNNNGC 7 cut(s) 457, 527, 730, 737, 1061, 1070, 1277
NciI CCSGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 909
NdeII GATC 4 cut(s) 268, 863, 872, 904
NheI GCTAGC 1 cut(s) 302
NlaIII CATG 6 cut(s) 293, 384, 451, 995, 1118, 1229
NlaIV GGNNCC 3 cut(s) 182, 630, 1036
NmuCI GTSAC 1 cut(s) 643
NspI RCATGY 1 cut(s) 995
PagI TCATGA 1 cut(s) 1114
PauI GCGCGC 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 4 cut(s) 104, 107, 116, 119
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 3 cut(s) 48, 880, 1301
PflMI CCANNNNNTGG 1 cut(s) 665
PkrI GCNGC 6 cut(s) 59, 131, 687, 730, 1057, 1075
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Ppu21I YACGTR 2 cut(s) 325, 390
Psp124BI GAGCTC 2 cut(s) 91, 199
PspN4I GGNNCC 3 cut(s) 182, 630, 1036
PspPI GGNCC 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 872
PteI GCGCGC 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 772
RsaI GTAC 4 cut(s) 282, 392, 708, 925
RsaNI GTAC 4 cut(s) 281, 391, 707, 924
SacI GAGCTC 2 cut(s) 91, 199
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 365, 957
SatI GCNGC 6 cut(s) 58, 130, 686, 729, 1056, 1074
Sau3AI GATC 4 cut(s) 268, 863, 872, 904
Sau96I GGNCC 1 cut(s) 1034
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 409
SduI GDGCHC 2 cut(s) 91, 199
SfaNI GCATC 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 9 cut(s) 169, 335, 565, 580, 637, 735, 844, 951, 1119
SsiI CCGC 3 cut(s) 9, 254, 806
SspMI CTAG 4 cut(s) 92, 303, 350, 506
SstI GAGCTC 2 cut(s) 91, 199
StyD4I CCNGG 1 cut(s) 407
StyI CCWWGG 3 cut(s) 184, 1104, 1196
TaaI ACNGT 1 cut(s) 1155
TaiI ACGT 3 cut(s) 327, 392, 1278
TaqI TCGA 2 cut(s) 67, 757
TasI AATT 9 cut(s) 169, 335, 565, 580, 637, 735, 844, 951, 1119
TfiI GAWTC 3 cut(s) 48, 880, 1301
Tru1I TTAA 2 cut(s) 365, 957
Tru9I TTAA 2 cut(s) 365, 957
TscAI CASTG 2 cut(s) 559, 787
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 6 cut(s) 57, 129, 685, 728, 1055, 1073
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 8 cut(s) 278, 369, 456, 1131, 1146, 1175, 1214, 1251
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 2 cut(s) 559, 787
Van91I CCANNNNNTGG 1 cut(s) 665
XapI RAATTY 3 cut(s) 580, 844, 951
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 4 cut(s) 92, 303, 350, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.