Rw0G000600

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00020
OG0004186
Physical Location & Seq
Reverse (-)
48900 .. 52128
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G000600.1

Sequence Viewer

Length: 1335 bp
ATGGCCTCCGCTCCTCTAATCCACGCGCGCTGTGGTTACTCGCCCAGATTCAGTAGCAGCAGTAATCGACGGAATGGAGGAGGAGGAGCTCTAGGTTCGTCGCCGTCGTCGTCTTCTTCGCCTTCGTCGTCGTGTTGCTCTTCTTCGTATTTGGCCTTCTCTTCTGGGGCGTATAATTGCTGGAGGAACCAAGGACTCAGAGCTCACGCTATGACCAGTCAGGGGAATTTTGCTTCTTCCTCCAGGGGTGTTGCGAGTGCGGGGAAGAAGCCCGATCATCTTCTCGTACTTGTTCATGGCATATTGGCTAGCCCAAGTGATTGGACATACGTGGAAGCAGAATTACGGAAGCGTCTAGGCAGAAGCTTCTTAATCTATGCAAGTTCATGCAACACGTACACAAAAACTTTTGCCGGGATTGATGGAGCTGGAAAGAGACTGGCAGATGAAGTCATGCAAGTTGTGCAAAAGACAGATAGCCTCAAGAAAATCTCATTCTTAGCCCATTCTCTAGGGGGCTTGTTTGCAAGATACGCAATATCTGTTCTTTACAGACCCAGTGATATTCCCAATTCACCTCTTGTAAATTCAAAAGCAACACACTCTTTGAGACGAGGAAAGATTGCCGGTTTGGAGCCAATCAATTTTGTAACATTGGCAACCCCACATCTTGGGGTGAGGGGGAAAAAGCAGCTTCCATTTCTTTTAGGCGTACCTTTGTTAGAAAAGCTGGCTGCGCCAATTGCTCCTATTGTTGTTGGTCGAACTGGTAGTCAGCTGTTCCTCACTGATGGTAAACCTGAAAAACCACCGCTTCTTTTGAGAATGGCATCTGATTGTGAAGATGGAAATTTTGTATCTGCATTGGGATCGTTTAGATCTCGGATTCTTTATGCTAATGTATCTTATGATCATATGGTTGGTTGGCGTACATCATCCATAAGGAGAGAGACAGAAATTTTTAAGCCTCCTCGCCGGTCTTTGGTTGGCTACAAGCATGTTGTAGATGTAGAGTATTGTCCCCCAGTTTCCTCTGACGGCCCCCATTTTCCTCCTGAAGCAGCTAAGGCGAAGGAGGCAGCCCAAAGTAAACCCAACCAACAGAACACCTTGGAATATCATGAAATTGTTGAAGATGAAATGATACGAGGCTTACAACAGTTGGGATGGAAAAAAGTTGATGTCAGCTTTCATTCTGCACCTTGGCCATTCTTTGCCCACAACAACATTCATGTAAAAAATGAATGGCTTCACAATGCTGGTGCTGGAGTGGTTGCACACGTTGCTGACACTCTAAAGCAACAAGAATCCTCCACATTGACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

444

Amino Acids

48.14

Weight (kDa)

9.59

Isoelectric Point (pI)

50.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 89 - 311 1.3e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 11
AccII CGCG 2 cut(s) 26, 28
AciI CCGC 3 cut(s) 9, 260, 812
AclWI GGATC 1 cut(s) 877
AcoI YGGCCR 1 cut(s) 1205
AcsI RAATTY 4 cut(s) 226, 586, 850, 957
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 4 cut(s) 288, 398, 714, 931
AfiI CCNNNNNNNGG 3 cut(s) 222, 671, 982
AflIII ACRYGT 2 cut(s) 393, 1279
AgsI TTSAA 2 cut(s) 591, 1133
AjnI CCWGG 1 cut(s) 242
Alw21I GWGCWC 2 cut(s) 91, 205
Alw26I GTCTC 3 cut(s) 430, 604, 944
AlwI GGATC 1 cut(s) 877
AoxI GGCC 4 cut(s) 3, 153, 1039, 1205
ApeKI GCWGC 5 cut(s) 57, 691, 734, 1061, 1079
ApoI RAATTY 4 cut(s) 226, 586, 850, 957
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
Asp700I GAANNNNTTC 1 cut(s) 1248
AspLEI GCGC 3 cut(s) 28, 30, 739
AspS9I GGNCC 1 cut(s) 1040
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 567, 688
AsuNHI GCTAGC 1 cut(s) 308
BalI TGGCCA 1 cut(s) 1207
BanII GRGCYC 2 cut(s) 91, 205
BarI GAAGNNNNNNTAC 4 cut(s) 140, 172, 327, 359
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 2 cut(s) 91, 205
BbvI GCAGC 5 cut(s) 69, 703, 721, 1073, 1091
BccI CCATC 4 cut(s) 416, 785, 839, 1161
BceAI ACGGC 2 cut(s) 88, 1054
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 244
BclI TGATCA 1 cut(s) 910
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 3 cut(s) 430, 604, 944
BfaI CTAG 4 cut(s) 92, 309, 356, 512
BglII AGATCT 1 cut(s) 878
BisI GCNGC 5 cut(s) 58, 692, 735, 1062, 1080
BlsI GCNGC 5 cut(s) 59, 693, 736, 1063, 1081
Bme1390I CCNGG 2 cut(s) 244, 415
BmgT120I GGNCC 1 cut(s) 1040
BmiI GGNNCC 3 cut(s) 188, 636, 1042
BmrFI CCNGG 2 cut(s) 244, 415
BmrI ACTGGG 2 cut(s) 552, 1019
BmsI GCATC 1 cut(s) 839
BmtI GCTAGC 1 cut(s) 312
BmuI ACTGGG 2 cut(s) 552, 1019
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 3 cut(s) 202, 226, 1287
Bpu10I CCTNAGC 1 cut(s) 1065
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 415
BsaAI YACGTR 2 cut(s) 331, 396
BsaJI CCNNGG 4 cut(s) 190, 243, 1110, 1202
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 671, 982
Bse118I RCCGGY 2 cut(s) 626, 975
Bse1I ACTGG 5 cut(s) 216, 444, 558, 772, 1025
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 4 cut(s) 190, 243, 1110, 1202
BseGI GGATG 2 cut(s) 935, 1172
BseLI CCNNNNNNNGG 3 cut(s) 222, 671, 982
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 5 cut(s) 216, 444, 558, 772, 1025
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 4 cut(s) 93, 96, 99, 960
BseXI GCAGC 5 cut(s) 69, 703, 721, 1073, 1091
BsgI GTGCAG 1 cut(s) 1182
Bsh1236I CGCG 2 cut(s) 26, 28
BshFI GGCC 4 cut(s) 5, 155, 1041, 1207
BsiHKAI GWGCWC 2 cut(s) 91, 205
BsiSI CCGG 3 cut(s) 414, 627, 976
BslFI GGGAC 1 cut(s) 1005
BslI CCNNNNNNNGG 3 cut(s) 222, 671, 982
BsmAI GTCTC 3 cut(s) 430, 604, 944
BsmBI CGTCTC 1 cut(s) 604
BsmFI GGGAC 1 cut(s) 1005
BsnI GGCC 4 cut(s) 5, 155, 1041, 1207
Bsp1286I GDGCHC 2 cut(s) 91, 205
Bsp143I GATC 4 cut(s) 274, 869, 878, 910
BspACI CCGC 3 cut(s) 9, 260, 812
BspANI GGCC 4 cut(s) 5, 155, 1041, 1207
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 2 cut(s) 26, 28
BspHI TCATGA 1 cut(s) 1120
BspLI GGNNCC 3 cut(s) 188, 636, 1042
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 877
BspQI GCTCTTC 1 cut(s) 145
BsrBI CCGCTC 1 cut(s) 11
BsrFI RCCGGY 2 cut(s) 626, 975
BsrI ACTGG 5 cut(s) 216, 444, 558, 772, 1025
BssAI RCCGGY 2 cut(s) 626, 975
BssECI CCNNGG 4 cut(s) 190, 243, 1110, 1202
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 4 cut(s) 274, 869, 878, 910
BssT1I CCWWGG 3 cut(s) 190, 1110, 1202
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 1 cut(s) 1161
Bst6I CTCTTC 2 cut(s) 145, 166
BstAPI GCANNNNNTGC 2 cut(s) 463, 1283
BstBAI YACGTR 2 cut(s) 331, 396
BstC8I GCNNGC 4 cut(s) 28, 310, 732, 1327
BstDEI CTNAG 3 cut(s) 197, 499, 1065
BstF5I GGATG 2 cut(s) 935, 1172
BstFNI CGCG 2 cut(s) 26, 28
BstHHI GCGC 3 cut(s) 28, 30, 739
BstKTI GATC 4 cut(s) 277, 872, 881, 913
BstMAI GTCTC 3 cut(s) 430, 604, 944
BstMBI GATC 4 cut(s) 274, 869, 878, 910
BstMWI GCNNNNNNNGC 7 cut(s) 463, 533, 736, 743, 1067, 1076, 1283
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 1001
BstSCI CCNGG 2 cut(s) 242, 413
BstUI CGCG 2 cut(s) 26, 28
BstV1I GCAGC 5 cut(s) 69, 703, 721, 1073, 1091
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 878
BstXI CCANNNNNNTGG 1 cut(s) 321
BstYI RGATCY 1 cut(s) 878
BsuRI GGCC 4 cut(s) 5, 155, 1041, 1207
BtsCI GGATG 2 cut(s) 935, 1172
BtsIMutI CAGTG 2 cut(s) 565, 786
Cac8I GCNNGC 4 cut(s) 28, 310, 732, 1327
CciI TCATGA 1 cut(s) 1120
CfoI GCGC 3 cut(s) 28, 30, 739
Cfr10I RCCGGY 2 cut(s) 626, 975
Cfr13I GGNCC 1 cut(s) 1040
CseI GACGC 1 cut(s) 341
Csp6I GTAC 4 cut(s) 287, 397, 713, 930
CviAII CATG 6 cut(s) 296, 387, 454, 998, 1121, 1232
CviQI GTAC 4 cut(s) 287, 397, 713, 930
DdeI CTNAG 3 cut(s) 197, 499, 1065
DpnI GATC 4 cut(s) 276, 871, 880, 912
DpnII GATC 4 cut(s) 274, 869, 878, 910
EaeI YGGCCR 1 cut(s) 1205
Eam1104I CTCTTC 2 cut(s) 145, 166
EarI CTCTTC 2 cut(s) 145, 166
Ecl136II GAGCTC 2 cut(s) 89, 203
Eco130I CCWWGG 3 cut(s) 190, 1110, 1202
Eco24I GRGCYC 2 cut(s) 91, 205
Eco53kI GAGCTC 2 cut(s) 89, 203
Eco57I CTGAAG 1 cut(s) 1077
EcoICRI GAGCTC 2 cut(s) 89, 203
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 3 cut(s) 190, 1110, 1202
EcoT38I GRGCYC 2 cut(s) 91, 205
ErhI CCWWGG 3 cut(s) 190, 1110, 1202
Esp3I CGTCTC 1 cut(s) 604
FaeI CATG 6 cut(s) 299, 390, 457, 1001, 1124, 1235
FaqI GGGAC 1 cut(s) 1005
FatI CATG 6 cut(s) 295, 386, 453, 997, 1120, 1231
FauI CCCGC 1 cut(s) 253
FauNDI CATATG 1 cut(s) 915
FbaI TGATCA 1 cut(s) 910
Fnu4HI GCNGC 5 cut(s) 58, 692, 735, 1062, 1080
FokI GGATG 2 cut(s) 922, 1179
FriOI GRGCYC 2 cut(s) 91, 205
Fsp4HI GCNGC 5 cut(s) 58, 692, 735, 1062, 1080
FspBI CTAG 4 cut(s) 92, 309, 356, 512
GlaI GCGC 3 cut(s) 27, 29, 738
GluI GCNGC 5 cut(s) 58, 692, 735, 1062, 1080
GsuI CTGGAG 3 cut(s) 202, 226, 1287
HaeIII GGCC 4 cut(s) 5, 155, 1041, 1207
HapII CCGG 3 cut(s) 414, 627, 976
HgaI GACGC 1 cut(s) 341
HhaI GCGC 3 cut(s) 28, 30, 739
Hin1II CATG 6 cut(s) 299, 390, 457, 1001, 1124, 1235
Hin6I GCGC 3 cut(s) 26, 28, 737
HinP1I GCGC 3 cut(s) 26, 28, 737
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 4 cut(s) 48, 195, 886, 1307
HpaII CCGG 3 cut(s) 414, 627, 976
HphI GGTGA 2 cut(s) 567, 688
Hpy166II GTNNAC 3 cut(s) 399, 797, 1091
Hpy188I TCNGA 4 cut(s) 200, 835, 885, 1036
Hpy188III TCNNGA 3 cut(s) 484, 1055, 1121
Hpy8I GTNNAC 3 cut(s) 399, 797, 1091
Hpy99I CGWCG 6 cut(s) 72, 103, 109, 112, 130, 133
HpyAV CCTTC 3 cut(s) 132, 166, 1066
HpyCH4III ACNGT 1 cut(s) 1161
HpyCH4IV ACGT 3 cut(s) 330, 395, 1281
HpyCH4V TGCA 8 cut(s) 380, 390, 457, 466, 527, 863, 1199, 1277
HpyF10VI GCNNNNNNNGC 7 cut(s) 463, 533, 736, 743, 1067, 1076, 1283
HpyF3I CTNAG 3 cut(s) 197, 499, 1065
HpySE526I ACGT 3 cut(s) 330, 395, 1281
Hsp92II CATG 6 cut(s) 299, 390, 457, 1001, 1124, 1235
HspAI GCGC 3 cut(s) 26, 28, 737
Ksp22I TGATCA 1 cut(s) 910
Kzo9I GATC 4 cut(s) 274, 869, 878, 910
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 5 cut(s) 16, 86, 425, 634, 751
Lsp1109I GCAGC 5 cut(s) 69, 703, 721, 1073, 1091
LweI GCATC 1 cut(s) 839
MaeI CTAG 4 cut(s) 92, 309, 356, 512
MaeII ACGT 3 cut(s) 330, 395, 1281
MaeIII GTNAC 2 cut(s) 35, 649
MalI GATC 4 cut(s) 276, 871, 880, 912
MbiI CCGCTC 1 cut(s) 11
MboI GATC 4 cut(s) 274, 869, 878, 910
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 878
MhlI GDGCHC 2 cut(s) 91, 205
MlsI TGGCCA 1 cut(s) 1207
MluNI TGGCCA 1 cut(s) 1207
MlyI GAGTC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 1207
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 1207
MseI TTAA 2 cut(s) 371, 963
Msp20I TGGCCA 1 cut(s) 1207
MspA1I CMGCKG 1 cut(s) 778
MspI CCGG 3 cut(s) 414, 627, 976
MspR9I CCNGG 2 cut(s) 244, 415
MunI CAATTG 1 cut(s) 741
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 2 cut(s) 26, 28
MwoI GCNNNNNNNGC 7 cut(s) 463, 533, 736, 743, 1067, 1076, 1283
NciI CCSGG 1 cut(s) 415
NdeI CATATG 1 cut(s) 915
NdeII GATC 4 cut(s) 274, 869, 878, 910
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 6 cut(s) 299, 390, 457, 1001, 1124, 1235
NlaIV GGNNCC 3 cut(s) 188, 636, 1042
NspI RCATGY 1 cut(s) 1001
PagI TCATGA 1 cut(s) 1120
PauI GCGCGC 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 3 cut(s) 104, 107, 125
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 3 cut(s) 48, 886, 1307
PflMI CCANNNNNTGG 1 cut(s) 671
PkrI GCNGC 5 cut(s) 59, 693, 736, 1063, 1081
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
Ppu21I YACGTR 2 cut(s) 331, 396
Psp124BI GAGCTC 2 cut(s) 91, 205
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 188, 636, 1042
PspPI GGNCC 1 cut(s) 1040
PsuI RGATCY 1 cut(s) 878
PteI GCGCGC 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 4 cut(s) 288, 398, 714, 931
RsaNI GTAC 4 cut(s) 287, 397, 713, 930
SacI GAGCTC 2 cut(s) 91, 205
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 371, 963
SatI GCNGC 5 cut(s) 58, 692, 735, 1062, 1080
Sau3AI GATC 4 cut(s) 274, 869, 878, 910
Sau96I GGNCC 1 cut(s) 1040
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 2 cut(s) 244, 415
SduI GDGCHC 2 cut(s) 91, 205
SfaNI GCATC 1 cut(s) 839
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SsiI CCGC 3 cut(s) 9, 260, 812
SspMI CTAG 4 cut(s) 92, 309, 356, 512
SstI GAGCTC 2 cut(s) 91, 205
StyD4I CCNGG 2 cut(s) 242, 413
StyI CCWWGG 3 cut(s) 190, 1110, 1202
TaaI ACNGT 1 cut(s) 1161
TaiI ACGT 3 cut(s) 333, 398, 1284
TaqI TCGA 2 cut(s) 67, 763
TfiI GAWTC 3 cut(s) 48, 886, 1307
Tru1I TTAA 2 cut(s) 371, 963
Tru9I TTAA 2 cut(s) 371, 963
TscAI CASTG 2 cut(s) 565, 793
TseI GCWGC 5 cut(s) 57, 691, 734, 1061, 1079
TspDTI ATGAA 8 cut(s) 284, 375, 462, 1137, 1152, 1181, 1220, 1257
TspGWI ACGGA 2 cut(s) 85, 361
TspRI CASTG 2 cut(s) 565, 793
Van91I CCANNNNNTGG 1 cut(s) 671
XapI RAATTY 4 cut(s) 226, 586, 850, 957
XceI RCATGY 1 cut(s) 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 4 cut(s) 92, 309, 356, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.