Rh5BG079000

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004186
Physical Location & Seq
Forward (+)
6670892 .. 6675656
4765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG079000.1

Sequence Viewer

Length: 1320 bp
ATGGCCTCAGCTCCTCTAATCCACGCGCGCTGCGGTTACTCGCCCAGATTCAGTAGCAGCAGTAATCGACGGAATGGAGGAGGAGGAGCTCTAGGTTCGTCGCCGTCGTCGTCTTCGTGTTGCTCTTCTTCGTATTTGGCCTTCTCTTCTGGGGCGTATAATTGCTGGAGGAACCAAGGACTCAGAGCTCTCGCTATGACCAGTCAGGGGAATTTTGCTTCTTCTTCCAGAGGTGTTGCGAGTGCGGAGAAGAAGCCTGATCATCTTCTCGTACTTGTTCATGGCATATTGGCTAGCCCAAGTGATTGGACATACGTGGAAGCAGAATTACGAAAGCGTCTAGGCAGAAGCTTCTTAATCTATGCAAGTTCATGCAACACGTACACAAAAACTTTTACCGGGATTGATGGAGCTGGAAAGAGACTGGCAGATGAAGTCATGCAAGTTGTGCAAAAGACTGATAGCCTGAAGAAAATCTCATTCTTAGCCCATTCTCTAGGGGGCTTGTTTGCAAGATATGCAATATCTGTTCTTTACAGACCCAGTGATATTCCCAATTCACCTCTTGTAAATTCAAAAGCAACACACTCTTTGAGACGAGGAAAGATTGCCGGTTTGGAGCCAATCAATTTTGTAACATTGGCAACCCCACATCTTGGGGTGAGGGGGAAAAAGCAGCTTCCATTTCTTTTAGGCGTACCATTGTTAGAAAAGCTGGCTGCGCCAATTGCTCCTATTGTTGTTGGTCGAACTGGTAGTCAGCTGTTCCTCACTGATGGTAAACCTGAAAAACCACCGCTTCTTTTGAGAATGGCATCTGATTGTGAAGATGGAAATTTTGTATCTGCATTGGGATCGTTTAGATCTCGGATTCTTTATGCTAATGTATCTTATGATCATATGGTTGGTTGGCGTACATCATCCATAAGGAGAGAGACAGAAATTTTTAAGCCTCCTCGCCGGTCTTTGGTTGGCTACAAGCATGTTGTAGATGTGGAGTATTGTCCCCCAGTTTCCTCTGACGGCCCCCATTTTCCTCCTGAAGCAGCTAAGGCGAAGGAGGCAGCCCAAAGTAAACCCAACCAACAGAACACTTTGGAATATCATGAAATTGTTGAAGATGAAATGATACGAGGCTTACAACAGTTGGGATGGAAAAAAGTTGATGTCAGCTTTCATTCTGCACCTTGGCCATTCTTCGCCCACAACAACATTCATGTAAAAAATGAATGGCTTCACAATGCTGGTGCTGGAGTGGTTGCACACGTTGCTGACACTCTAAAGCAACAAGAATCCTCCACATTGACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

439

Amino Acids

47.78

Weight (kDa)

9.54

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 84 - 306 1.3e-65 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 656
AccII CGCG 2 cut(s) 26, 28
AciI CCGC 3 cut(s) 33, 245, 797
AclWI GGATC 1 cut(s) 862
AcoI YGGCCR 1 cut(s) 1190
AcsI RAATTY 4 cut(s) 211, 571, 835, 942
AcuI CTGAAG 2 cut(s) 488, 1062
AfaI GTAC 4 cut(s) 273, 383, 699, 916
AfiI CCNNNNNNNGG 3 cut(s) 207, 656, 967
AflIII ACRYGT 2 cut(s) 378, 1264
AgsI TTSAA 2 cut(s) 576, 1118
Alw21I GWGCWC 2 cut(s) 91, 190
Alw26I GTCTC 3 cut(s) 415, 589, 929
AlwI GGATC 1 cut(s) 862
AoxI GGCC 4 cut(s) 3, 138, 1024, 1190
ApeKI GCWGC 6 cut(s) 30, 57, 676, 719, 1046, 1064
ApoI RAATTY 4 cut(s) 211, 571, 835, 942
ArsI GACNNNNNNTTYG 2 cut(s) 742, 774
Asp700I GAANNNNTTC 1 cut(s) 1233
AspLEI GCGC 3 cut(s) 28, 30, 724
AspS9I GGNCC 1 cut(s) 1025
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 552, 673
AsuNHI GCTAGC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 1192
BanII GRGCYC 2 cut(s) 91, 190
BarI GAAGNNNNNNTAC 4 cut(s) 125, 157, 312, 344
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 2 cut(s) 91, 190
BbvCI CCTCAGC 1 cut(s) 7
BbvI GCAGC 6 cut(s) 17, 69, 688, 706, 1058, 1076
BccI CCATC 4 cut(s) 401, 770, 824, 1146
BceAI ACGGC 2 cut(s) 88, 1039
BcgI CGANNNNNNTGC 2 cut(s) 837, 871
BclI TGATCA 2 cut(s) 259, 895
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 3 cut(s) 415, 589, 929
BfaI CTAG 4 cut(s) 92, 294, 341, 497
BglII AGATCT 1 cut(s) 863
BisI GCNGC 6 cut(s) 31, 58, 677, 720, 1047, 1065
BlsI GCNGC 6 cut(s) 32, 59, 678, 721, 1048, 1066
Bme1390I CCNGG 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 1025
BmiI GGNNCC 3 cut(s) 173, 621, 1027
BmrFI CCNGG 1 cut(s) 400
BmrI ACTGGG 2 cut(s) 537, 1004
BmsI GCATC 1 cut(s) 824
BmtI GCTAGC 1 cut(s) 297
BmuI ACTGGG 2 cut(s) 537, 1004
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 2 cut(s) 187, 1272
Bpu10I CCTNAGC 2 cut(s) 7, 1050
BpuMI CCSGG 1 cut(s) 400
BsaAI YACGTR 2 cut(s) 316, 381
BsaJI CCNNGG 2 cut(s) 175, 1187
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 656, 967
Bse118I RCCGGY 2 cut(s) 611, 960
Bse1I ACTGG 5 cut(s) 201, 429, 543, 757, 1010
BseDI CCNNGG 2 cut(s) 175, 1187
BseGI GGATG 2 cut(s) 920, 1157
BseLI CCNNNNNNNGG 3 cut(s) 207, 656, 967
BseMII CTCAG 2 cut(s) 21, 196
BseNI ACTGG 5 cut(s) 201, 429, 543, 757, 1010
BsePI GCGCGC 1 cut(s) 26
BseRI GAGGAG 4 cut(s) 93, 96, 99, 945
BseXI GCAGC 6 cut(s) 17, 69, 688, 706, 1058, 1076
BsgI GTGCAG 1 cut(s) 1167
Bsh1236I CGCG 2 cut(s) 26, 28
BshFI GGCC 4 cut(s) 5, 140, 1026, 1192
BsiHKAI GWGCWC 2 cut(s) 91, 190
BsiSI CCGG 3 cut(s) 399, 612, 961
BslFI GGGAC 1 cut(s) 990
BslI CCNNNNNNNGG 3 cut(s) 207, 656, 967
BsmAI GTCTC 3 cut(s) 415, 589, 929
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 990
BsnI GGCC 4 cut(s) 5, 140, 1026, 1192
Bsp1286I GDGCHC 2 cut(s) 91, 190
Bsp143I GATC 4 cut(s) 259, 854, 863, 895
BspACI CCGC 3 cut(s) 33, 245, 797
BspANI GGCC 4 cut(s) 5, 140, 1026, 1192
BspCNI CTCAG 2 cut(s) 20, 195
BspFNI CGCG 2 cut(s) 26, 28
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 3 cut(s) 173, 621, 1027
BspOI GCTAGC 1 cut(s) 297
BspPI GGATC 1 cut(s) 862
BspQI GCTCTTC 1 cut(s) 130
BsrFI RCCGGY 2 cut(s) 611, 960
BsrI ACTGG 5 cut(s) 201, 429, 543, 757, 1010
BssAI RCCGGY 2 cut(s) 611, 960
BssECI CCNNGG 2 cut(s) 175, 1187
BssHII GCGCGC 1 cut(s) 26
BssMI GATC 4 cut(s) 259, 854, 863, 895
BssT1I CCWWGG 2 cut(s) 175, 1187
Bst4CI ACNGT 1 cut(s) 1146
Bst6I CTCTTC 2 cut(s) 130, 151
BstAPI GCANNNNNTGC 3 cut(s) 448, 518, 1268
BstBAI YACGTR 2 cut(s) 316, 381
BstC8I GCNNGC 4 cut(s) 28, 295, 717, 1312
BstDEI CTNAG 4 cut(s) 7, 182, 484, 1050
BstF5I GGATG 2 cut(s) 920, 1157
BstFNI CGCG 2 cut(s) 26, 28
BstHHI GCGC 3 cut(s) 28, 30, 724
BstKTI GATC 4 cut(s) 262, 857, 866, 898
BstMAI GTCTC 3 cut(s) 415, 589, 929
BstMBI GATC 4 cut(s) 259, 854, 863, 895
BstMWI GCNNNNNNNGC 7 cut(s) 448, 518, 721, 728, 1052, 1061, 1268
BstNSI RCATGY 1 cut(s) 986
BstSCI CCNGG 1 cut(s) 398
BstUI CGCG 2 cut(s) 26, 28
BstV1I GCAGC 6 cut(s) 17, 69, 688, 706, 1058, 1076
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 863
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 1 cut(s) 863
BsuRI GGCC 4 cut(s) 5, 140, 1026, 1192
BtsCI GGATG 2 cut(s) 920, 1157
BtsIMutI CAGTG 2 cut(s) 550, 771
Cac8I GCNNGC 4 cut(s) 28, 295, 717, 1312
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 3 cut(s) 28, 30, 724
Cfr10I RCCGGY 2 cut(s) 611, 960
Cfr13I GGNCC 1 cut(s) 1025
CseI GACGC 1 cut(s) 326
Csp6I GTAC 4 cut(s) 272, 382, 698, 915
CviAII CATG 6 cut(s) 281, 372, 439, 983, 1106, 1217
CviQI GTAC 4 cut(s) 272, 382, 698, 915
DdeI CTNAG 4 cut(s) 7, 182, 484, 1050
DpnI GATC 4 cut(s) 261, 856, 865, 897
DpnII GATC 4 cut(s) 259, 854, 863, 895
EaeI YGGCCR 1 cut(s) 1190
Eam1104I CTCTTC 2 cut(s) 130, 151
EarI CTCTTC 2 cut(s) 130, 151
Ecl136II GAGCTC 2 cut(s) 89, 188
Eco130I CCWWGG 2 cut(s) 175, 1187
Eco24I GRGCYC 2 cut(s) 91, 190
Eco53kI GAGCTC 2 cut(s) 89, 188
Eco57I CTGAAG 2 cut(s) 488, 1062
EcoICRI GAGCTC 2 cut(s) 89, 188
EcoT14I CCWWGG 2 cut(s) 175, 1187
EcoT38I GRGCYC 2 cut(s) 91, 190
ErhI CCWWGG 2 cut(s) 175, 1187
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 6 cut(s) 284, 375, 442, 986, 1109, 1220
FaqI GGGAC 1 cut(s) 990
FatI CATG 6 cut(s) 280, 371, 438, 982, 1105, 1216
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 2 cut(s) 259, 895
Fnu4HI GCNGC 6 cut(s) 31, 58, 677, 720, 1047, 1065
FokI GGATG 2 cut(s) 907, 1164
FriOI GRGCYC 2 cut(s) 91, 190
Fsp4HI GCNGC 6 cut(s) 31, 58, 677, 720, 1047, 1065
FspBI CTAG 4 cut(s) 92, 294, 341, 497
GlaI GCGC 3 cut(s) 27, 29, 723
GluI GCNGC 6 cut(s) 31, 58, 677, 720, 1047, 1065
GsuI CTGGAG 2 cut(s) 187, 1272
HaeIII GGCC 4 cut(s) 5, 140, 1026, 1192
HapII CCGG 3 cut(s) 399, 612, 961
HgaI GACGC 1 cut(s) 326
HhaI GCGC 3 cut(s) 28, 30, 724
Hin1II CATG 6 cut(s) 284, 375, 442, 986, 1109, 1220
Hin6I GCGC 3 cut(s) 26, 28, 722
HinP1I GCGC 3 cut(s) 26, 28, 722
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 4 cut(s) 48, 180, 871, 1292
HpaII CCGG 3 cut(s) 399, 612, 961
HphI GGTGA 2 cut(s) 552, 673
Hpy166II GTNNAC 3 cut(s) 384, 782, 1076
Hpy188I TCNGA 4 cut(s) 185, 820, 870, 1021
Hpy188III TCNNGA 3 cut(s) 228, 1040, 1106
Hpy8I GTNNAC 3 cut(s) 384, 782, 1076
Hpy99I CGWCG 4 cut(s) 72, 103, 109, 112
HpyAV CCTTC 2 cut(s) 151, 1051
HpyCH4III ACNGT 1 cut(s) 1146
HpyCH4IV ACGT 3 cut(s) 315, 380, 1266
HpyCH4V TGCA 9 cut(s) 365, 375, 442, 451, 512, 521, 848, 1184, 1262
HpyF10VI GCNNNNNNNGC 7 cut(s) 448, 518, 721, 728, 1052, 1061, 1268
HpyF3I CTNAG 4 cut(s) 7, 182, 484, 1050
HpySE526I ACGT 3 cut(s) 315, 380, 1266
Hsp92II CATG 6 cut(s) 284, 375, 442, 986, 1109, 1220
HspAI GCGC 3 cut(s) 26, 28, 722
Ksp22I TGATCA 2 cut(s) 259, 895
Kzo9I GATC 4 cut(s) 259, 854, 863, 895
LguI GCTCTTC 1 cut(s) 130
LmnI GCTCC 5 cut(s) 16, 86, 410, 619, 736
Lsp1109I GCAGC 6 cut(s) 17, 69, 688, 706, 1058, 1076
LweI GCATC 1 cut(s) 824
MaeI CTAG 4 cut(s) 92, 294, 341, 497
MaeII ACGT 3 cut(s) 315, 380, 1266
MaeIII GTNAC 2 cut(s) 35, 634
MalI GATC 4 cut(s) 261, 856, 865, 897
MboI GATC 4 cut(s) 259, 854, 863, 895
MfeI CAATTG 1 cut(s) 726
MflI RGATCY 1 cut(s) 863
MhlI GDGCHC 2 cut(s) 91, 190
MlsI TGGCCA 1 cut(s) 1192
MluNI TGGCCA 1 cut(s) 1192
MlyI GAGTC 1 cut(s) 174
Mox20I TGGCCA 1 cut(s) 1192
MroXI GAANNNNTTC 1 cut(s) 1233
MscI TGGCCA 1 cut(s) 1192
MseI TTAA 2 cut(s) 356, 948
Msp20I TGGCCA 1 cut(s) 1192
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 3 cut(s) 399, 612, 961
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 726
MvnI CGCG 2 cut(s) 26, 28
MwoI GCNNNNNNNGC 7 cut(s) 448, 518, 721, 728, 1052, 1061, 1268
NciI CCSGG 1 cut(s) 400
NdeI CATATG 1 cut(s) 900
NdeII GATC 4 cut(s) 259, 854, 863, 895
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 6 cut(s) 284, 375, 442, 986, 1109, 1220
NlaIV GGNNCC 3 cut(s) 173, 621, 1027
NspI RCATGY 1 cut(s) 986
PagI TCATGA 1 cut(s) 1105
PauI GCGCGC 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 130
PcsI WCGNNNNNNNCGW 3 cut(s) 104, 107, 113
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 3 cut(s) 48, 871, 1292
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 6 cut(s) 32, 59, 678, 721, 1048, 1066
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
Ppu21I YACGTR 2 cut(s) 316, 381
Psp124BI GAGCTC 2 cut(s) 91, 190
PspN4I GGNNCC 3 cut(s) 173, 621, 1027
PspPI GGNCC 1 cut(s) 1025
PsuI RGATCY 1 cut(s) 863
PteI GCGCGC 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 4 cut(s) 273, 383, 699, 916
RsaNI GTAC 4 cut(s) 272, 382, 698, 915
SacI GAGCTC 2 cut(s) 91, 190
SapI GCTCTTC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 356, 948
SatI GCNGC 6 cut(s) 31, 58, 677, 720, 1047, 1065
Sau3AI GATC 4 cut(s) 259, 854, 863, 895
Sau96I GGNCC 1 cut(s) 1025
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 2 cut(s) 91, 190
SfaNI GCATC 1 cut(s) 824
SsiI CCGC 3 cut(s) 33, 245, 797
SspMI CTAG 4 cut(s) 92, 294, 341, 497
SstI GAGCTC 2 cut(s) 91, 190
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 2 cut(s) 175, 1187
TaaI ACNGT 1 cut(s) 1146
TaiI ACGT 3 cut(s) 318, 383, 1269
TaqI TCGA 2 cut(s) 67, 748
TfiI GAWTC 3 cut(s) 48, 871, 1292
Tru1I TTAA 2 cut(s) 356, 948
Tru9I TTAA 2 cut(s) 356, 948
TscAI CASTG 2 cut(s) 550, 778
TseI GCWGC 6 cut(s) 30, 57, 676, 719, 1046, 1064
TspDTI ATGAA 8 cut(s) 269, 360, 447, 1122, 1137, 1166, 1205, 1242
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 2 cut(s) 550, 778
Van91I CCANNNNNTGG 1 cut(s) 656
XapI RAATTY 4 cut(s) 211, 571, 835, 942
XceI RCATGY 1 cut(s) 986
XmnI GAANNNNTTC 1 cut(s) 1233
XspI CTAG 4 cut(s) 92, 294, 341, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.