MD10G1280400.v1.1

Putative serine esterase (DUF676)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004186
Physical Location & Seq
Reverse (-)
37123669 .. 37127244
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1280400.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGCGTCCCCTTCTCCGATTCACCCGCGCTTAGGGCACTTACCGAGTTTCAGCTGCAATCGGCGAAACGACTCCCAAGGACCTTTAGGCACCTCCTCCTCCTCTTCGTCGTTCAGCTTCGGTTCCGATATTGCAGGCATATGTGGTAGTTGGAGAAACCAAGGCCTTAGAGCTCAAGCTATGACTAGTCAGGGAAATTTTGGATCCTCCAGGGGTGTTGCAAATGCGGAGAATAAACCCGATCATCTTCTTGTCCTTGTTCACGGCATCTTGGCGAGCCCGAGTGACTGGACATACGTGGAAGCAGAGTTAAAAAGGCGTCTAGGAAGAAACTTCTTAATCTATGCAAGTTCGTGTAACACGTACACCAAAACTTTTTCTGGTATTGATGGAGCTGGGAAACGATTAGCAGATGAAGTTATGCAACTTGTACAAAAGACAGAGAGCCTAAAGAAGATCTCATTTTTAGCCCATTCTCTAGGGGGTTTATTTGCAAGATATGCAATTTCTGTCCTTTACAACGATAGTGCCAGTTCAACTACTGCAAATTCACAAACGGCATACTCTTTGAGACAAGGAAAGATTGCTGGGTTAGAGCCTATTAATTTTGTAACATTGGCAACTCCACATCTTGGGATGCGAGGGAAAAAGCAGCTTCCATTTTTATTAGGAGTACCTTTCTTAGAAAAGCTGGCTGCACCAATCGCTCCTATTGTTGTTGGTCGAACTGGCAGTCAATTGTTTCTCACCGATGGTAAGCCTGAAAGACCACCACTTCTTCTGAGAATGGCATCCGATTGCAAAGATGGGAAATTTTTATCTGCTTTGGGGTCATTCAAATCACGGATTATCTATGCAAATGTATCTTATGATCATATGGTTGGTTGGCGCACATCATCCATTAGGAGGGAGACAGAAATTTTTAAGCCCTCTCGCGGGTCTTTGGACGGGTACAAGCATGTTGTAGACGTGGAGTACTGTCCTCCAGTTTCATCTGAAGGTCCCCATTTTCCTCCTGAAGCAGCTAAAGCAAAGGAAGCCGCCCAAAGCGAACCCAACGTACAAAATACGTTGGAATATCACGAAATTGTAGAAGATGAAATGATACGGGGTTTGCAGCAACTAGGATGGAGAAAAGTTGATGTCAACTTTCATTCGGCGTTCTGGCCCTTCTTTGCCCACAACAACATTCATGTGAAAAGTGAATGGCTTCACAATGCTGGCGTCGGGGTGGTTGCTCACGTTGCCGACAGCCTGAAGCAACAGGAATCCTCCTCCTCATTCATCACTGCCAGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF676

Protein Analysis

434

Amino Acids

47.3

Weight (kDa)

9.15

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF676 PF05057 78 - 298 1.3e-66 Putative serine esterase (DUF676)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 1 cut(s) 966
AccII CGCG 2 cut(s) 28, 936
AciI CCGC 4 cut(s) 26, 227, 936, 1041
AclWI GGATC 2 cut(s) 198, 211
AcsI RAATTY 4 cut(s) 196, 547, 812, 918
AcuI CTGAAG 3 cut(s) 1017, 1038, 1277
AcyI GRCGYC 3 cut(s) 5, 319, 1224
AfaI GTAC 6 cut(s) 365, 432, 675, 953, 977, 1062
AfiI CCNNNNNNNGG 5 cut(s) 32, 632, 906, 935, 936
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 2 cut(s) 537, 838
AhlI ACTAGT 1 cut(s) 185
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 209
AluBI AGCT 9 cut(s) 54, 117, 173, 179, 395, 655, 691, 1025, 1296
AluI AGCT 9 cut(s) 54, 117, 173, 179, 395, 655, 691, 1025, 1296
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 2 cut(s) 565, 905
AlwI GGATC 2 cut(s) 198, 211
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 2 cut(s) 163, 1166
ApeKI GCWGC 5 cut(s) 54, 652, 695, 1022, 1117
ApoI RAATTY 4 cut(s) 196, 547, 812, 918
ArsI GACNNNNNNTTYG 2 cut(s) 718, 750
AseI ATTAAT 1 cut(s) 603
Asp700I GAANNNNTTC 1 cut(s) 1209
AspLEI GCGC 2 cut(s) 30, 891
AspS9I GGNCC 3 cut(s) 80, 1001, 1167
AsuHPI GGTGA 2 cut(s) 14, 739
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 2 cut(s) 80, 1001
BaeGI GKGCMC 1 cut(s) 39
BamHI GGATCC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 175, 281
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 5 cut(s) 41, 664, 682, 1034, 1129
BccI CCATC 4 cut(s) 383, 746, 800, 1122
BceAI ACGGC 2 cut(s) 280, 573
BciT130I CCWGG 1 cut(s) 211
BclI TGATCA 1 cut(s) 871
BcoDI GTCTC 2 cut(s) 565, 905
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 4 cut(s) 186, 323, 479, 1124
BglII AGATCT 1 cut(s) 456
BisI GCNGC 6 cut(s) 55, 653, 696, 1023, 1041, 1118
BlsI GCNGC 6 cut(s) 56, 654, 697, 1024, 1042, 1119
BmcAI AGTACT 1 cut(s) 977
Bme1390I CCNGG 1 cut(s) 211
Bme18I GGWCC 2 cut(s) 80, 1001
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 3 cut(s) 80, 1001, 1167
BmiI GGNNCC 4 cut(s) 91, 124, 205, 1003
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 3 cut(s) 276, 627, 800
BpmI CTGGAG 2 cut(s) 193, 969
Bpu10I CCTNAGC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 159
BsaAI YACGTR 2 cut(s) 298, 363
BsaHI GRCGYC 3 cut(s) 5, 319, 1224
BsaJI CCNNGG 3 cut(s) 76, 160, 210
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 632, 906, 935, 936
Bse1I ACTGG 4 cut(s) 293, 531, 733, 986
BseBI CCWGG 1 cut(s) 211
BseDI CCNNGG 3 cut(s) 76, 160, 210
BseGI GGATG 4 cut(s) 642, 791, 896, 1133
BseLI CCNNNNNNNGG 5 cut(s) 32, 632, 906, 935, 936
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 4 cut(s) 293, 531, 733, 986
BseRI GAGGAG 5 cut(s) 85, 88, 91, 1264, 1267
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 5 cut(s) 41, 664, 682, 1034, 1129
BseYI CCCAGC 2 cut(s) 395, 587
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 2 cut(s) 28, 936
BshFI GGCC 2 cut(s) 165, 1168
BshNI GGYRCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 280
BslFI GGGAC 1 cut(s) 987
BslI CCNNNNNNNGG 5 cut(s) 32, 632, 906, 935, 936
BsmAI GTCTC 2 cut(s) 565, 905
BsmFI GGGAC 1 cut(s) 987
BsnI GGCC 2 cut(s) 165, 1168
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 3 cut(s) 39, 175, 281
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 203, 241, 456, 871
BspACI CCGC 4 cut(s) 26, 227, 936, 1041
BspANI GGCC 2 cut(s) 165, 1168
BspCNI CTCAG 1 cut(s) 774
BspFNI CGCG 2 cut(s) 28, 936
BspLI GGNNCC 4 cut(s) 91, 124, 205, 1003
BspPI GGATC 2 cut(s) 198, 211
BspT107I GGYRCC 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 4 cut(s) 293, 531, 733, 986
BssECI CCNNGG 3 cut(s) 76, 160, 210
BssMI GATC 4 cut(s) 203, 241, 456, 871
BssNI GRCGYC 3 cut(s) 5, 319, 1224
BssT1I CCWWGG 2 cut(s) 76, 160
Bst2UI CCWGG 1 cut(s) 211
Bst4CI ACNGT 1 cut(s) 980
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 3 cut(s) 5, 319, 1224
BstAPI GCANNNNNTGC 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 430
BstBAI YACGTR 2 cut(s) 298, 363
BstC8I GCNNGC 5 cut(s) 136, 277, 693, 1222, 1294
BstDEI CTNAG 4 cut(s) 31, 167, 682, 782
BstF5I GGATG 4 cut(s) 642, 791, 896, 1133
BstFNI CGCG 2 cut(s) 28, 936
BstHHI GCGC 2 cut(s) 30, 891
BstKTI GATC 4 cut(s) 206, 244, 459, 874
BstMAI GTCTC 2 cut(s) 565, 905
BstMBI GATC 4 cut(s) 203, 241, 456, 871
BstMWI GCNNNNNNNGC 6 cut(s) 34, 500, 704, 1028, 1037, 1244
BstNI CCWGG 1 cut(s) 211
BstNSI RCATGY 1 cut(s) 962
BstSCI CCNGG 1 cut(s) 209
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 2 cut(s) 28, 936
BstV1I GCAGC 5 cut(s) 41, 664, 682, 1034, 1129
BstX2I RGATCY 2 cut(s) 203, 456
BstYI RGATCY 2 cut(s) 203, 456
BsuRI GGCC 2 cut(s) 165, 1168
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 4 cut(s) 642, 791, 896, 1133
BtsI GCAGTG 1 cut(s) 1287
BtsIMutI CAGTG 1 cut(s) 1287
Cac8I GCNNGC 5 cut(s) 136, 277, 693, 1222, 1294
CfoI GCGC 2 cut(s) 30, 891
Cfr13I GGNCC 3 cut(s) 80, 1001, 1167
CseI GACGC 2 cut(s) 308, 1213
Csp6I GTAC 6 cut(s) 364, 431, 674, 952, 976, 1061
CviAII CATG 2 cut(s) 959, 1193
CviQI GTAC 6 cut(s) 364, 431, 674, 952, 976, 1061
DdeI CTNAG 4 cut(s) 31, 167, 682, 782
DpnI GATC 4 cut(s) 205, 243, 458, 873
DpnII GATC 4 cut(s) 203, 241, 456, 871
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 76, 160
Eco147I AGGCCT 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 175, 281
Eco47I GGWCC 2 cut(s) 80, 1001
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 3 cut(s) 1017, 1038, 1277
Eco88I CYCGRG 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 173
EcoO109I RGGNCCY 2 cut(s) 80, 1001
EcoRII CCWGG 1 cut(s) 209
EcoT14I CCWWGG 2 cut(s) 76, 160
EcoT38I GRGCYC 2 cut(s) 175, 281
ErhI CCWWGG 2 cut(s) 76, 160
FaeI CATG 2 cut(s) 962, 1196
FaqI GGGAC 1 cut(s) 987
FatI CATG 2 cut(s) 958, 1192
FauI CCCGC 2 cut(s) 33, 929
FauNDI CATATG 2 cut(s) 140, 876
FbaI TGATCA 1 cut(s) 871
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 6 cut(s) 55, 653, 696, 1023, 1041, 1118
FokI GGATG 4 cut(s) 649, 778, 883, 1140
FriOI GRGCYC 2 cut(s) 175, 281
Fsp4HI GCNGC 6 cut(s) 55, 653, 696, 1023, 1041, 1118
FspBI CTAG 4 cut(s) 186, 323, 479, 1124
GlaI GCGC 2 cut(s) 29, 890
GluI GCNGC 6 cut(s) 55, 653, 696, 1023, 1041, 1118
GsaI CCCAGC 2 cut(s) 399, 591
GsuI CTGGAG 2 cut(s) 193, 969
HaeIII GGCC 2 cut(s) 165, 1168
HgaI GACGC 2 cut(s) 308, 1213
HhaI GCGC 2 cut(s) 30, 891
Hin1I GRCGYC 3 cut(s) 5, 319, 1224
Hin1II CATG 2 cut(s) 962, 1196
Hin6I GCGC 2 cut(s) 28, 889
HinP1I GCGC 2 cut(s) 28, 889
HincII GTYRAC 1 cut(s) 1147
HindII GTYRAC 1 cut(s) 1147
HinfI GANTC 3 cut(s) 19, 71, 1268
HphI GGTGA 2 cut(s) 14, 739
Hpy166II GTNNAC 4 cut(s) 262, 366, 967, 1147
Hpy188I TCNGA 5 cut(s) 18, 127, 783, 796, 997
Hpy188III TCNNGA 2 cut(s) 1016, 1082
Hpy8I GTNNAC 4 cut(s) 262, 366, 967, 1147
Hpy99I CGWCG 2 cut(s) 112, 1229
HpyAV CCTTC 3 cut(s) 21, 992, 1180
HpyCH4III ACNGT 1 cut(s) 980
HpyCH4IV ACGT 6 cut(s) 297, 362, 969, 1059, 1070, 1242
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 500, 704, 1028, 1037, 1244
HpyF3I CTNAG 4 cut(s) 31, 167, 682, 782
HpySE526I ACGT 6 cut(s) 297, 362, 969, 1059, 1070, 1242
Hsp92I GRCGYC 3 cut(s) 5, 319, 1224
Hsp92II CATG 2 cut(s) 962, 1196
HspAI GCGC 2 cut(s) 28, 889
Ksp22I TGATCA 1 cut(s) 871
Kzo9I GATC 4 cut(s) 203, 241, 456, 871
LmnI GCTCC 2 cut(s) 392, 712
Lsp1109I GCAGC 5 cut(s) 41, 664, 682, 1034, 1129
LweI GCATC 3 cut(s) 276, 627, 800
MaeI CTAG 4 cut(s) 186, 323, 479, 1124
MaeII ACGT 6 cut(s) 297, 362, 969, 1059, 1070, 1242
MaeIII GTNAC 3 cut(s) 284, 356, 610
MalI GATC 4 cut(s) 205, 243, 458, 873
MboI GATC 4 cut(s) 203, 241, 456, 871
MboII GAAGA 6 cut(s) 96, 239, 339, 466, 770, 1106
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 2 cut(s) 203, 456
MhlI GDGCHC 3 cut(s) 39, 175, 281
MluCI AATT 8 cut(s) 196, 504, 547, 604, 737, 812, 918, 1086
MlyI GAGTC 1 cut(s) 65
MmeI TCCRAC 2 cut(s) 131, 1053
MroXI GAANNNNTTC 1 cut(s) 1209
MseI TTAA 4 cut(s) 311, 338, 603, 924
MslI CAYNNNNRTG 1 cut(s) 1193
MspA1I CMGCKG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 211
MunI CAATTG 1 cut(s) 737
MvaI CCWGG 1 cut(s) 211
MvnI CGCG 2 cut(s) 28, 936
MwoI GCNNNNNNNGC 6 cut(s) 34, 500, 704, 1028, 1037, 1244
NdeI CATATG 2 cut(s) 140, 876
NdeII GATC 4 cut(s) 203, 241, 456, 871
NlaIII CATG 2 cut(s) 962, 1196
NlaIV GGNNCC 4 cut(s) 91, 124, 205, 1003
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 962
PceI AGGCCT 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 2 cut(s) 19, 1268
PflMI CCANNNNNTGG 1 cut(s) 632
PkrI GCNGC 6 cut(s) 56, 654, 697, 1024, 1042, 1119
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Ppu21I YACGTR 2 cut(s) 298, 363
PpuMI RGGWCCY 2 cut(s) 80, 1001
PshBI ATTAAT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 175
Psp5II RGGWCCY 2 cut(s) 80, 1001
Psp6I CCWGG 1 cut(s) 209
PspFI CCCAGC 2 cut(s) 395, 587
PspGI CCWGG 1 cut(s) 209
PspN4I GGNNCC 4 cut(s) 91, 124, 205, 1003
PspPI GGNCC 3 cut(s) 80, 1001, 1167
PspPPI RGGWCCY 2 cut(s) 80, 1001
PsrI GAACNNNNNNTAC 2 cut(s) 1044, 1076
PsuI RGATCY 2 cut(s) 203, 456
PvuII CAGCTG 1 cut(s) 54
RsaI GTAC 6 cut(s) 365, 432, 675, 953, 977, 1062
RsaNI GTAC 6 cut(s) 364, 431, 674, 952, 976, 1061
RseI CAYNNNNRTG 1 cut(s) 1193
SacI GAGCTC 1 cut(s) 175
SaqAI TTAA 4 cut(s) 311, 338, 603, 924
SatI GCNGC 6 cut(s) 55, 653, 696, 1023, 1041, 1118
Sau3AI GATC 4 cut(s) 203, 241, 456, 871
Sau96I GGNCC 3 cut(s) 80, 1001, 1167
ScaI AGTACT 1 cut(s) 977
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 3 cut(s) 39, 175, 281
SfaNI GCATC 3 cut(s) 276, 627, 800
SinI GGWCC 2 cut(s) 80, 1001
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 1 cut(s) 174
SmoI CTYRAG 1 cut(s) 174
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 8 cut(s) 196, 504, 547, 604, 737, 812, 918, 1086
SseBI AGGCCT 1 cut(s) 165
SsiI CCGC 4 cut(s) 26, 227, 936, 1041
SspMI CTAG 4 cut(s) 186, 323, 479, 1124
SstI GAGCTC 1 cut(s) 175
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 209
StyI CCWWGG 2 cut(s) 76, 160
TaaI ACNGT 1 cut(s) 980
TaiI ACGT 6 cut(s) 300, 365, 972, 1062, 1073, 1245
TaqI TCGA 1 cut(s) 724
TasI AATT 8 cut(s) 196, 504, 547, 604, 737, 812, 918, 1086
TatI WGTACW 2 cut(s) 430, 975
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 2 cut(s) 19, 1268
Tru1I TTAA 4 cut(s) 311, 338, 603, 924
Tru9I TTAA 4 cut(s) 311, 338, 603, 924
TscAI CASTG 1 cut(s) 1294
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 5 cut(s) 54, 652, 695, 1022, 1117
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 6 cut(s) 429, 981, 1113, 1142, 1181, 1273
TspGWI ACGGA 1 cut(s) 859
TspRI CASTG 1 cut(s) 1294
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 2 cut(s) 80, 1001
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 4 cut(s) 196, 547, 812, 918
XceI RCATGY 1 cut(s) 962
XmiI GTMKAC 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 4 cut(s) 186, 323, 479, 1124
ZrmI AGTACT 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.