MD05G1303200.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008821
Physical Location & Seq
Reverse (-)
43585095 .. 43586430
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1303200.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCTTCAGTTTCAGCAATGTCGTGGTCGCCGTACCTTTCCACTCGAGCCTCCACACCCTGTGCTTCTCTCACATTCCTCACCGGCTCCACAACTAGGAAGAGCTCCGTGTCTCTCAGCGGTGGCGCCGCCGCCGCGCGTTCCCGGTTGCTTCACTGCTCGTTCATCTCCTCCTCCTCTATCGCTTCCACTTCCGCCTTCTCAGTGTTGGGGTTGGAAGAAGAAAGAGGCTCTGGCTTAGTGGTGAGGGCCGGGAAGGCTGCCCTGTGTCTAACCAAGAGAAGTAGGTCCAGGAAATCCTTGGCTCGAACTCATGGATTCCGTAGGCGCATGAGAACAACCGGCGGGAGAGCAATGATGAAGCGCCGACGTGCTAAGGGGCGGAAGGTCCTCTGCACGAAGAGCAATCCCAGCAGTGGAAAACGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

144

Amino Acids

15.21

Weight (kDa)

12.0

Isoelectric Point (pI)

64.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 102 - 132 1.9e-09 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AccII CGCG 2 cut(s) 137, 139
AciI CCGC 7 cut(s) 120, 129, 132, 135, 195, 345, 382
AcyI GRCGYC 1 cut(s) 126
AdeI CACNNNGTG 1 cut(s) 62
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 2 cut(s) 96, 416
AjiI CACGTC 1 cut(s) 371
AjnI CCWGG 1 cut(s) 290
AluBI AGCT 1 cut(s) 105
AluI AGCT 1 cut(s) 105
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 260
AspLEI GCGC 4 cut(s) 128, 139, 330, 366
AspS9I GGNCC 3 cut(s) 249, 288, 388
AsuC2I CCSGG 2 cut(s) 145, 253
AsuHPI GGTGA 2 cut(s) 73, 256
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 2 cut(s) 288, 388
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 292
BcnI CCSGG 2 cut(s) 145, 253
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 96
BfoI RGCGCY 2 cut(s) 129, 367
BglI GCCNNNNNGGC 1 cut(s) 257
BisI GCNGC 4 cut(s) 129, 132, 135, 261
BlsI GCNGC 4 cut(s) 130, 133, 136, 262
Bme1390I CCNGG 3 cut(s) 145, 253, 292
Bme18I GGWCC 2 cut(s) 288, 388
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 371
BmgT120I GGNCC 3 cut(s) 249, 288, 388
BmiI GGNNCC 2 cut(s) 88, 127
BmrFI CCNGG 3 cut(s) 145, 253, 292
Bpu10I CCTNAGC 1 cut(s) 375
BpuMI CCSGG 2 cut(s) 145, 253
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 416
Bse118I RCCGGY 2 cut(s) 83, 341
Bse3DI GCAATG 2 cut(s) 24, 360
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 1 cut(s) 300
BseLI CCNNNNNNNGG 2 cut(s) 96, 416
BseMI GCAATG 2 cut(s) 24, 360
BseMII CTCAG 2 cut(s) 130, 216
BseRI GAGGAG 3 cut(s) 160, 163, 166
BseXI GCAGC 1 cut(s) 247
BseYI CCCAGC 1 cut(s) 410
BsgI GTGCAG 1 cut(s) 379
Bsh1236I CGCG 2 cut(s) 137, 139
BshFI GGCC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 4 cut(s) 84, 145, 252, 342
BslI CCNNNNNNNGG 2 cut(s) 96, 416
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 107
BspACI CCGC 7 cut(s) 120, 129, 132, 135, 195, 345, 382
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 2 cut(s) 129, 215
BspFNI CGCG 2 cut(s) 137, 139
BspLI GGNNCC 2 cut(s) 88, 127
BspQI GCTCTTC 2 cut(s) 95, 395
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 24, 360
BsrFI RCCGGY 2 cut(s) 83, 341
BssAI RCCGGY 2 cut(s) 83, 341
BssECI CCNNGG 1 cut(s) 300
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 292
Bst6I CTCTTC 2 cut(s) 95, 395
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 4 cut(s) 116, 202, 238, 375
BstFNI CGCG 2 cut(s) 137, 139
BstH2I RGCGCY 2 cut(s) 129, 367
BstHHI GCGC 4 cut(s) 128, 139, 330, 366
BstMAI GTCTC 1 cut(s) 117
BstMWI GCNNNNNNNGC 4 cut(s) 134, 257, 402, 411
BstNI CCWGG 1 cut(s) 292
BstSCI CCNGG 3 cut(s) 143, 251, 290
BstUI CGCG 2 cut(s) 137, 139
BstV1I GCAGC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 251
BtrI CACGTC 1 cut(s) 371
BtsI GCAGTG 2 cut(s) 154, 421
BtsIMutI CAGTG 3 cut(s) 154, 210, 421
CfoI GCGC 4 cut(s) 128, 139, 330, 366
Cfr10I RCCGGY 2 cut(s) 83, 341
Cfr13I GGNCC 3 cut(s) 249, 288, 388
Csp6I GTAC 1 cut(s) 34
CviAII CATG 2 cut(s) 314, 331
CviJI RGCY 9 cut(s) 5, 50, 87, 105, 231, 237, 251, 260, 305
CviKI_1 RGCY 9 cut(s) 5, 50, 87, 105, 231, 237, 251, 260, 305
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 4 cut(s) 116, 202, 238, 375
DinI GGCGCC 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 62
Eam1104I CTCTTC 2 cut(s) 95, 395
EarI CTCTTC 2 cut(s) 95, 395
EciI GGCGGA 2 cut(s) 184, 397
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 288, 388
Eco53kI GAGCTC 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 107
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 317, 334
FaiI YATR 2 cut(s) 315, 332
FatI CATG 2 cut(s) 313, 330
FauI CCCGC 1 cut(s) 338
Fnu4HI GCNGC 4 cut(s) 129, 132, 135, 261
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 4 cut(s) 129, 132, 135, 261
FspBI CTAG 1 cut(s) 96
GlaI GCGC 4 cut(s) 127, 138, 329, 365
GluI GCNGC 4 cut(s) 129, 132, 135, 261
GsaI CCCAGC 1 cut(s) 414
HaeII RGCGCY 2 cut(s) 129, 367
HaeIII GGCC 1 cut(s) 251
HapII CCGG 4 cut(s) 84, 145, 252, 342
HhaI GCGC 4 cut(s) 128, 139, 330, 366
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 2 cut(s) 317, 334
Hin6I GCGC 4 cut(s) 126, 137, 328, 364
HinP1I GCGC 4 cut(s) 126, 137, 328, 364
HinfI GANTC 1 cut(s) 318
HpaII CCGG 4 cut(s) 84, 145, 252, 342
HphI GGTGA 2 cut(s) 73, 256
Hpy99I CGWCG 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 208, 250, 379
HpyCH4IV ACGT 2 cut(s) 370, 424
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 257, 402, 411
HpyF3I CTNAG 4 cut(s) 116, 202, 238, 375
HpySE526I ACGT 2 cut(s) 370, 424
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 2 cut(s) 317, 334
HspAI GCGC 4 cut(s) 126, 137, 328, 364
KasI GGCGCC 1 cut(s) 125
LguI GCTCTTC 2 cut(s) 95, 395
LmnI GCTCC 2 cut(s) 92, 110
Lsp1109I GCAGC 1 cut(s) 247
MaeI CTAG 1 cut(s) 96
MaeII ACGT 2 cut(s) 370, 424
MboII GAAGA 4 cut(s) 112, 230, 233, 412
MhlI GDGCHC 1 cut(s) 107
Mly113I GGCGCC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 8 cut(s) 61, 89, 181, 184, 187, 221, 240, 401
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 4 cut(s) 84, 145, 252, 342
MspR9I CCNGG 3 cut(s) 145, 253, 292
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 2 cut(s) 137, 139
MwoI GCNNNNNNNGC 4 cut(s) 134, 257, 402, 411
NarI GGCGCC 1 cut(s) 126
NciI CCSGG 2 cut(s) 145, 253
NlaIII CATG 2 cut(s) 317, 334
NlaIV GGNNCC 2 cut(s) 88, 127
PaeR7I CTCGAG 1 cut(s) 45
PciSI GCTCTTC 2 cut(s) 95, 395
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PfeI GAWTC 1 cut(s) 318
PfoI TCCNGGA 1 cut(s) 290
PkrI GCNGC 4 cut(s) 130, 133, 136, 262
PluTI GGCGCC 1 cut(s) 129
PpuMI RGGWCCY 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 290
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 2 cut(s) 88, 127
PspPI GGNCC 3 cut(s) 249, 288, 388
PspPPI RGGWCCY 1 cut(s) 388
PspXI VCTCGAGB 1 cut(s) 45
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SacI GAGCTC 1 cut(s) 107
SapI GCTCTTC 2 cut(s) 95, 395
SatI GCNGC 4 cut(s) 129, 132, 135, 261
Sau96I GGNCC 3 cut(s) 249, 288, 388
ScrFI CCNGG 3 cut(s) 145, 253, 292
SduI GDGCHC 1 cut(s) 107
SetI ASST 6 cut(s) 39, 107, 290, 373, 390, 427
SfoI GGCGCC 1 cut(s) 127
Sfr274I CTCGAG 1 cut(s) 45
SinI GGWCC 2 cut(s) 288, 388
SlaI CTCGAG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
SsiI CCGC 7 cut(s) 120, 129, 132, 135, 195, 345, 382
SspDI GGCGCC 1 cut(s) 125
SspMI CTAG 1 cut(s) 96
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 143, 251, 290
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 2 cut(s) 373, 427
TaqI TCGA 2 cut(s) 46, 307
TauI GCSGC 3 cut(s) 131, 134, 137
TfiI GAWTC 1 cut(s) 318
TscAI CASTG 3 cut(s) 161, 210, 421
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 154, 374
TspGWI ACGGA 2 cut(s) 97, 311
TspRI CASTG 3 cut(s) 161, 210, 421
VpaK11BI GGWCC 2 cut(s) 288, 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 45
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.