MD10G1282300.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008821
Physical Location & Seq
Reverse (-)
37294979 .. 37296404
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1282300.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGCTTCAATTTCAGCAATGGCATGGTCGCCCTGCCTTTCCAGTCGAGCCTCCACACCCTGTGCTTCTCTCACATTCCTCACCGGCTCAACTGCAAGGAAGAGCTCCGTGTCTTTCAGCGGTGGCGCCGCCGCGCGCTCCCCCTTGCTTCACTGCTCCTTCATCTCTTCCTCCTCCATCGCTTCCGCTTCTGCGTTCTCGGGTTTATCTTTGGGGTTGGATTTGAGCTCTAGTGTTGGGGTTGGAAGAAGAAGAGGCTCTGGCTTGGTGGTGAGGGCCGGGAAGGCTGCCCTGTGTCTAACCAAGAGAAGTAGGTCCAGGAAATCCTTGGCTCGAACTCATGGCTTCCGTAGACGCATGAGAACCACTGGTGGGAGAGCAATGATGAAGCGCCGACGTGCCAAGGGGCGGAAGGTCCTCTGCACGAAGAGCAATCCCAGTAGTGGAAAACGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

153

Amino Acids

15.86

Weight (kDa)

12.0

Isoelectric Point (pI)

69.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 111 - 141 1.8e-09 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 352
AccII CGCG 2 cut(s) 134, 136
AciI CCGC 5 cut(s) 120, 129, 132, 186, 409
AcyI GRCGYC 1 cut(s) 126
AdeI CACNNNGTG 1 cut(s) 62
AfiI CCNNNNNNNGG 3 cut(s) 372, 408, 443
AgsI TTSAA 1 cut(s) 9
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 2 cut(s) 105, 228
AluI AGCT 2 cut(s) 105, 228
Alw21I GWGCWC 2 cut(s) 107, 230
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 2 cut(s) 276, 453
ApeKI GCWGC 1 cut(s) 287
AspLEI GCGC 4 cut(s) 128, 136, 138, 393
AspS9I GGNCC 4 cut(s) 276, 315, 415, 453
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 73, 283
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 315, 415
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 107, 230
Bbv12I GWGCWC 2 cut(s) 107, 230
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 319
BcnI CCSGG 1 cut(s) 280
BfaI CTAG 1 cut(s) 231
BfoI RGCGCY 2 cut(s) 129, 394
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 129, 132, 288
BlsI GCNGC 3 cut(s) 130, 133, 289
Bme1390I CCNGG 2 cut(s) 280, 319
Bme18I GGWCC 2 cut(s) 315, 415
BmeT110I CYCGRG 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 4 cut(s) 276, 315, 415, 453
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 280, 319
BmrI ACTGGG 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 280
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 327, 402
Bsc4I CCNNNNNNNGG 3 cut(s) 372, 408, 443
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 3 cut(s) 42, 373, 438
Bse3DI GCAATG 2 cut(s) 24, 387
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 2 cut(s) 327, 402
BseLI CCNNNNNNNGG 3 cut(s) 372, 408, 443
BseMI GCAATG 2 cut(s) 24, 387
BseNI ACTGG 3 cut(s) 42, 373, 438
BsePI GCGCGC 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 2 cut(s) 134, 136
BshFI GGCC 2 cut(s) 278, 455
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 107, 230
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 2 cut(s) 84, 279
BslI CCNNNNNNNGG 3 cut(s) 372, 408, 443
BsnI GGCC 2 cut(s) 278, 455
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 107, 230
BspACI CCGC 5 cut(s) 120, 129, 132, 186, 409
BspANI GGCC 2 cut(s) 278, 455
BspFNI CGCG 2 cut(s) 134, 136
BspLI GGNNCC 1 cut(s) 127
BspQI GCTCTTC 2 cut(s) 95, 422
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 24, 387
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 3 cut(s) 42, 373, 438
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 327, 402
BssHII GCGCGC 1 cut(s) 134
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 327, 402
Bst2UI CCWGG 1 cut(s) 319
Bst6I CTCTTC 4 cut(s) 95, 172, 247, 422
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 136
BstFNI CGCG 2 cut(s) 134, 136
BstH2I RGCGCY 2 cut(s) 129, 394
BstHHI GCGC 4 cut(s) 128, 136, 138, 393
BstMWI GCNNNNNNNGC 2 cut(s) 284, 429
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 278, 317
BstUI CGCG 2 cut(s) 134, 136
BstV1I GCAGC 1 cut(s) 274
BsuRI GGCC 2 cut(s) 278, 455
BtgZI GCGATG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 151, 366
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 4 cut(s) 128, 136, 138, 393
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 276, 315, 415, 453
CseI GACGC 1 cut(s) 363
CviAII CATG 3 cut(s) 24, 341, 358
DinI GGCGCC 1 cut(s) 127
DraIII CACNNNGTG 1 cut(s) 62
Eam1104I CTCTTC 4 cut(s) 95, 172, 247, 422
EarI CTCTTC 4 cut(s) 95, 172, 247, 422
EciI GGCGGA 1 cut(s) 424
Ecl136II GAGCTC 2 cut(s) 105, 228
Eco130I CCWWGG 2 cut(s) 327, 402
Eco24I GRGCYC 2 cut(s) 107, 230
Eco47I GGWCC 2 cut(s) 315, 415
Eco53kI GAGCTC 2 cut(s) 105, 228
Eco88I CYCGRG 1 cut(s) 199
EcoICRI GAGCTC 2 cut(s) 105, 228
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 2 cut(s) 327, 402
EcoT38I GRGCYC 2 cut(s) 107, 230
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 2 cut(s) 327, 402
FaeI CATG 3 cut(s) 27, 344, 361
FaiI YATR 3 cut(s) 25, 342, 359
FatI CATG 3 cut(s) 23, 340, 357
FblI GTMKAC 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 129, 132, 288
FriOI GRGCYC 2 cut(s) 107, 230
Fsp4HI GCNGC 3 cut(s) 129, 132, 288
FspBI CTAG 1 cut(s) 231
GlaI GCGC 4 cut(s) 127, 135, 137, 392
GluI GCNGC 3 cut(s) 129, 132, 288
HaeII RGCGCY 2 cut(s) 129, 394
HaeIII GGCC 2 cut(s) 278, 455
HapII CCGG 2 cut(s) 84, 279
HgaI GACGC 1 cut(s) 363
HhaI GCGC 4 cut(s) 128, 136, 138, 393
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 3 cut(s) 27, 344, 361
Hin6I GCGC 4 cut(s) 126, 134, 136, 391
HinP1I GCGC 4 cut(s) 126, 134, 136, 391
HpaII CCGG 2 cut(s) 84, 279
HphI GGTGA 2 cut(s) 73, 283
Hpy166II GTNNAC 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 353
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 3 cut(s) 169, 277, 406
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 2 cut(s) 95, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 429
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 3 cut(s) 27, 344, 361
HspAI GCGC 4 cut(s) 126, 134, 136, 391
KasI GGCGCC 1 cut(s) 125
LguI GCTCTTC 2 cut(s) 95, 422
LmnI GCTCC 3 cut(s) 110, 143, 161
Lsp1109I GCAGC 1 cut(s) 274
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 397
MboII GAAGA 6 cut(s) 112, 159, 258, 261, 264, 439
MhlI GDGCHC 2 cut(s) 107, 230
MluCI AATT 1 cut(s) 9
Mly113I GGCGCC 1 cut(s) 126
MmeI TCCRAC 2 cut(s) 198, 223
MnlI CCTC 7 cut(s) 61, 89, 181, 184, 248, 267, 428
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 2 cut(s) 84, 279
MspR9I CCNGG 2 cut(s) 280, 319
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 2 cut(s) 134, 136
MwoI GCNNNNNNNGC 2 cut(s) 284, 429
NarI GGCGCC 1 cut(s) 126
NciI CCSGG 1 cut(s) 280
NlaIII CATG 3 cut(s) 27, 344, 361
NlaIV GGNNCC 1 cut(s) 127
PauI GCGCGC 1 cut(s) 134
PciSI GCTCTTC 2 cut(s) 95, 422
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 3 cut(s) 130, 133, 289
PluTI GGCGCC 1 cut(s) 129
PpuMI RGGWCCY 1 cut(s) 415
Psp124BI GAGCTC 2 cut(s) 107, 230
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 4 cut(s) 276, 315, 415, 453
PspPPI RGGWCCY 1 cut(s) 415
PteI GCGCGC 1 cut(s) 134
SacI GAGCTC 2 cut(s) 107, 230
SapI GCTCTTC 2 cut(s) 95, 422
SatI GCNGC 3 cut(s) 129, 132, 288
Sau96I GGNCC 4 cut(s) 276, 315, 415, 453
ScrFI CCNGG 2 cut(s) 280, 319
SduI GDGCHC 2 cut(s) 107, 230
SetI ASST 5 cut(s) 107, 230, 317, 400, 417
SfoI GGCGCC 1 cut(s) 127
SinI GGWCC 2 cut(s) 315, 415
Sse9I AATT 1 cut(s) 9
SsiI CCGC 5 cut(s) 120, 129, 132, 186, 409
SspDI GGCGCC 1 cut(s) 125
SspMI CTAG 1 cut(s) 231
SstI GAGCTC 2 cut(s) 107, 230
StyD4I CCNGG 2 cut(s) 278, 317
StyI CCWWGG 2 cut(s) 327, 402
TaiI ACGT 1 cut(s) 400
TaqI TCGA 2 cut(s) 46, 334
TasI AATT 1 cut(s) 9
TauI GCSGC 2 cut(s) 131, 134
TscAI CASTG 2 cut(s) 158, 373
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 151, 401
TspGWI ACGGA 2 cut(s) 97, 338
TspRI CASTG 2 cut(s) 158, 373
VpaK11BI GGWCC 2 cut(s) 315, 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 352
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.