Rw5G007550

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008821
Physical Location & Seq
Forward (+)
7975118 .. 7976183
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007550.1

Sequence Viewer

Length: 450 bp
ATGGCTACACTCTCAGCAATTTCATGGTCACCTTCTCTCTCAACTAGACCCACAACTTCTGCTTGCCTCACATTCCTCACCGGAAGGAGAAGCTCCGTCTCTGTCAACGTTGCCGCCGCGCGTTCTTCGTTGCTTCACTGCTCCTTCATCCCCTCCTCTTCTGCTTCTCTCTCTTCCCCTTCTTCGTTTTCTGGTTTATCTTTGGGTTTGGATTTGATCTCTAATGTTGGGATTGGAAGAAGAAGAGGCTCTGGCTTAGTGGTGAGGGCTGGGAAGGCTGCCCTGTGTCTAACAAAGAGAAACAGGTCCCGGAAATCTCTGGCTCGAACTCATGGCTTCCGCAGACGCATGAGAACCACTAGTGGTAGAGCAATGTTGAAGCGCCGACGTGCCAAGGGGCGGAAGGTCCTCTGCACCAAGACCAATCCCAACAGTGGAAAACGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

149

Amino Acids

15.91

Weight (kDa)

12.0

Isoelectric Point (pI)

74.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 107 - 138 9e-11 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 119, 121
AciI CCGC 4 cut(s) 114, 117, 340, 400
AclI AACGTT 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 399, 434
AgsI TTSAA 1 cut(s) 379
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 389
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 278
AspLEI GCGC 2 cut(s) 121, 384
AspS9I GGNCC 2 cut(s) 306, 406
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 21, 70, 274
AvaII GGWCC 2 cut(s) 306, 406
BbvI GCAGC 1 cut(s) 265
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 103
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 2 cut(s) 45, 360
BfoI RGCGCY 1 cut(s) 385
BisI GCNGC 3 cut(s) 114, 117, 279
BlsI GCNGC 3 cut(s) 115, 118, 280
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 2 cut(s) 306, 406
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 306, 406
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 310
BpuMI CCSGG 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 2 cut(s) 399, 434
Bse3DI GCAATG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 2 cut(s) 399, 434
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 265
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 119, 121
BsiSI CCGG 2 cut(s) 81, 310
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 399, 434
BsmAI GTCTC 1 cut(s) 103
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 292
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 4 cut(s) 114, 117, 340, 400
BspCNI CTCAG 1 cut(s) 26
BspFNI CGCG 2 cut(s) 119, 121
BspLI GGNNCC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 3 cut(s) 163, 178, 238
BstC8I GCNNGC 1 cut(s) 64
BstDEI CTNAG 2 cut(s) 13, 256
BstEII GGTNACC 1 cut(s) 27
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 2 cut(s) 119, 121
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 2 cut(s) 121, 384
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 308
BstUI CGCG 2 cut(s) 119, 121
BstV1I GCAGC 1 cut(s) 265
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 1 cut(s) 147
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 439
Cac8I GCNNGC 1 cut(s) 64
CfoI GCGC 2 cut(s) 121, 384
Cfr13I GGNCC 2 cut(s) 306, 406
CseI GACGC 1 cut(s) 354
CviAII CATG 3 cut(s) 24, 332, 349
CviJI RGCY 8 cut(s) 5, 93, 249, 255, 269, 278, 323, 336
CviKI_1 RGCY 8 cut(s) 5, 93, 249, 255, 269, 278, 323, 336
DdeI CTNAG 2 cut(s) 13, 256
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Eam1104I CTCTTC 3 cut(s) 163, 178, 238
EarI CTCTTC 3 cut(s) 163, 178, 238
EciI GGCGGA 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 393
Eco47I GGWCC 2 cut(s) 306, 406
Eco91I GGTNACC 1 cut(s) 27
EcoO109I RGGNCCY 2 cut(s) 306, 406
EcoO65I GGTNACC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 3 cut(s) 27, 335, 352
FaiI YATR 3 cut(s) 25, 333, 350
FaqI GGGAC 1 cut(s) 292
FatI CATG 3 cut(s) 23, 331, 348
Fnu4HI GCNGC 3 cut(s) 114, 117, 279
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 114, 117, 279
FspBI CTAG 2 cut(s) 45, 360
GlaI GCGC 2 cut(s) 120, 383
GluI GCNGC 3 cut(s) 114, 117, 279
GsaI CCCAGC 1 cut(s) 273
HaeII RGCGCY 1 cut(s) 385
HapII CCGG 2 cut(s) 81, 310
HgaI GACGC 1 cut(s) 354
HhaI GCGC 2 cut(s) 121, 384
Hin1II CATG 3 cut(s) 27, 335, 352
Hin6I GCGC 2 cut(s) 119, 382
HinP1I GCGC 2 cut(s) 119, 382
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HpaII CCGG 2 cut(s) 81, 310
HphI GGTGA 3 cut(s) 21, 70, 274
Hpy166II GTNNAC 1 cut(s) 106
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 390
HpyAV CCTTC 6 cut(s) 42, 78, 154, 189, 268, 397
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4IV ACGT 3 cut(s) 108, 388, 442
HpyCH4V TGCA 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 2 cut(s) 13, 256
HpySE526I ACGT 3 cut(s) 108, 388, 442
Hsp92II CATG 3 cut(s) 27, 335, 352
HspAI GCGC 2 cut(s) 119, 382
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 2 cut(s) 98, 146
LpnPI CCDG 8 cut(s) 94, 177, 237, 255, 289, 296, 305, 323
Lsp1109I GCAGC 1 cut(s) 265
MaeI CTAG 2 cut(s) 45, 360
MaeII ACGT 3 cut(s) 108, 388, 442
MaeIII GTNAC 1 cut(s) 27
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 7 cut(s) 117, 150, 165, 174, 249, 252, 255
MluCI AATT 1 cut(s) 18
MnlI CCTC 7 cut(s) 77, 86, 163, 166, 239, 258, 419
MspI CCGG 2 cut(s) 81, 310
MspR9I CCNGG 1 cut(s) 310
MvnI CGCG 2 cut(s) 119, 121
MwoI GCNNNNNNNGC 1 cut(s) 275
NciI CCSGG 1 cut(s) 310
NdeII GATC 1 cut(s) 216
NlaIII CATG 3 cut(s) 27, 335, 352
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 27
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 3 cut(s) 115, 118, 280
PpuMI RGGWCCY 2 cut(s) 306, 406
Psp1406I AACGTT 1 cut(s) 108
Psp5II RGGWCCY 2 cut(s) 306, 406
PspEI GGTNACC 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 306, 406
PspPPI RGGWCCY 2 cut(s) 306, 406
SatI GCNGC 3 cut(s) 114, 117, 279
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 2 cut(s) 306, 406
ScrFI CCNGG 1 cut(s) 310
SetI ASST 7 cut(s) 34, 95, 111, 308, 391, 408, 445
SinI GGWCC 2 cut(s) 306, 406
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 1 cut(s) 18
SsiI CCGC 4 cut(s) 114, 117, 340, 400
SspMI CTAG 2 cut(s) 45, 360
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 434
TaiI ACGT 3 cut(s) 111, 391, 445
TaqI TCGA 1 cut(s) 325
TasI AATT 1 cut(s) 18
TauI GCSGC 2 cut(s) 116, 119
TscAI CASTG 2 cut(s) 143, 439
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 2 cut(s) 12, 136
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 2 cut(s) 143, 439
VpaK11BI GGWCC 2 cut(s) 306, 406
XspI CTAG 2 cut(s) 45, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.