pycom10g23550

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008821
Physical Location & Seq
Reverse (-)
25657157 .. 25658175
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23550.2

Sequence Viewer

Length: 459 bp
ATGGCTTCAATTTCAGCAATGGCATGGTCGCCCTGCCTTTCCAGTCGAGCCTCCACACCCTGTGCTTCTCTCACGTTCCTCACCGGCTCAACTGCAAGGAAGAGCTCCGTGTCTCTCAGCGGTGGCGCTGCCCCGCGCTCCCCGTTGCTTCACTGCTCCTTCATCTCTTCCTCCTCCATCGCTTCCGCTTCCGCGTTCTCGGGTTTATCTTTGGGGTTGGATTTGAGCTCTACTGTTGGGGTTGGAAGAAGAAGAGGCTCTGGCTTGGTGGTAAGGGCAGGGAAGGCTGCCCTGTGTCTAACCAAGAGAAGTAGGTCCAGGAAATCCTTGGCTCGAACTCATGGCTTCCGTAGACGCATGAGAACCACTGGTGGGAGAGCAATGATGAAGCGCCGACGTGCTAAGGGGCGGAAGGTCCTCTGCACGAAGAGCAATCCCAATAGTGGAAAACGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

153

Amino Acids

15.89

Weight (kDa)

12.0

Isoelectric Point (pI)

66.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 352
AccII CGCG 2 cut(s) 136, 194
AciI CCGC 5 cut(s) 120, 134, 186, 192, 409
AdeI CACNNNGTG 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 372, 443
AgsI TTSAA 1 cut(s) 9
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 2 cut(s) 105, 228
AluI AGCT 2 cut(s) 105, 228
Alw21I GWGCWC 2 cut(s) 107, 230
Alw26I GTCTC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 453
ApeKI GCWGC 2 cut(s) 128, 287
AspLEI GCGC 3 cut(s) 128, 138, 393
AspS9I GGNCC 3 cut(s) 315, 415, 453
AsuHPI GGTGA 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 315, 415
BanII GRGCYC 2 cut(s) 107, 230
Bbv12I GWGCWC 2 cut(s) 107, 230
BbvI GCAGC 2 cut(s) 115, 274
BccI CCATC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 117
BfoI RGCGCY 2 cut(s) 129, 394
BisI GCNGC 2 cut(s) 129, 288
BlsI GCNGC 2 cut(s) 130, 289
Bme1390I CCNGG 1 cut(s) 319
Bme18I GGWCC 2 cut(s) 315, 415
BmeT110I CYCGRG 1 cut(s) 199
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 315, 415, 453
BmrFI CCNGG 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 443
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 42, 373
Bse3DI GCAATG 2 cut(s) 24, 387
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 372, 443
BseMI GCAATG 2 cut(s) 24, 387
BseMII CTCAG 1 cut(s) 130
BseNI ACTGG 2 cut(s) 42, 373
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 2 cut(s) 115, 274
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 2 cut(s) 136, 194
BshFI GGCC 1 cut(s) 455
BsiHKAI GWGCWC 2 cut(s) 107, 230
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 372, 443
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 107, 230
BspACI CCGC 5 cut(s) 120, 134, 186, 192, 409
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 1 cut(s) 129
BspFNI CGCG 2 cut(s) 136, 194
BspQI GCTCTTC 2 cut(s) 95, 422
BsrDI GCAATG 2 cut(s) 24, 387
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 2 cut(s) 42, 373
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 327
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 4 cut(s) 95, 172, 247, 422
BstDEI CTNAG 2 cut(s) 116, 402
BstFNI CGCG 2 cut(s) 136, 194
BstH2I RGCGCY 2 cut(s) 129, 394
BstHHI GCGC 3 cut(s) 128, 138, 393
BstMAI GTCTC 1 cut(s) 117
BstMWI GCNNNNNNNGC 2 cut(s) 284, 429
BstNI CCWGG 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 317
BstUI CGCG 2 cut(s) 136, 194
BstV1I GCAGC 2 cut(s) 115, 274
BsuRI GGCC 1 cut(s) 455
BtgZI GCGATG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 151, 366
CfoI GCGC 3 cut(s) 128, 138, 393
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 315, 415, 453
CseI GACGC 1 cut(s) 363
CviAII CATG 3 cut(s) 24, 341, 358
DdeI CTNAG 2 cut(s) 116, 402
DraIII CACNNNGTG 1 cut(s) 62
Eam1104I CTCTTC 4 cut(s) 95, 172, 247, 422
EarI CTCTTC 4 cut(s) 95, 172, 247, 422
EciI GGCGGA 1 cut(s) 424
Ecl136II GAGCTC 2 cut(s) 105, 228
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 107, 230
Eco47I GGWCC 2 cut(s) 315, 415
Eco53kI GAGCTC 2 cut(s) 105, 228
Eco88I CYCGRG 1 cut(s) 199
EcoICRI GAGCTC 2 cut(s) 105, 228
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 107, 230
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 3 cut(s) 27, 344, 361
FaiI YATR 3 cut(s) 25, 342, 359
FatI CATG 3 cut(s) 23, 340, 357
FauI CCCGC 1 cut(s) 141
FblI GTMKAC 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 129, 288
FriOI GRGCYC 2 cut(s) 107, 230
Fsp4HI GCNGC 2 cut(s) 129, 288
GlaI GCGC 3 cut(s) 127, 137, 392
GluI GCNGC 2 cut(s) 129, 288
HaeII RGCGCY 2 cut(s) 129, 394
HaeIII GGCC 1 cut(s) 455
HapII CCGG 1 cut(s) 84
HgaI GACGC 1 cut(s) 363
HhaI GCGC 3 cut(s) 128, 138, 393
Hin1II CATG 3 cut(s) 27, 344, 361
Hin6I GCGC 3 cut(s) 126, 136, 391
HinP1I GCGC 3 cut(s) 126, 136, 391
HpaII CCGG 1 cut(s) 84
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 353
Hpy8I GTNNAC 1 cut(s) 353
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 3 cut(s) 169, 277, 406
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 2 cut(s) 74, 397
HpyCH4V TGCA 2 cut(s) 95, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 429
HpyF3I CTNAG 2 cut(s) 116, 402
HpySE526I ACGT 2 cut(s) 74, 397
Hsp92II CATG 3 cut(s) 27, 344, 361
HspAI GCGC 3 cut(s) 126, 136, 391
LguI GCTCTTC 2 cut(s) 95, 422
LmnI GCTCC 3 cut(s) 110, 143, 161
Lsp1109I GCAGC 2 cut(s) 115, 274
MaeII ACGT 2 cut(s) 74, 397
MboII GAAGA 6 cut(s) 112, 159, 258, 261, 264, 439
MhlI GDGCHC 2 cut(s) 107, 230
MluCI AATT 1 cut(s) 9
MmeI TCCRAC 2 cut(s) 198, 223
MnlI CCTC 6 cut(s) 61, 89, 181, 184, 248, 428
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 319
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 2 cut(s) 136, 194
MwoI GCNNNNNNNGC 2 cut(s) 284, 429
NlaIII CATG 3 cut(s) 27, 344, 361
PciSI GCTCTTC 2 cut(s) 95, 422
PfoI TCCNGGA 1 cut(s) 317
PkrI GCNGC 2 cut(s) 130, 289
PpuMI RGGWCCY 1 cut(s) 415
Psp124BI GAGCTC 2 cut(s) 107, 230
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspPI GGNCC 3 cut(s) 315, 415, 453
PspPPI RGGWCCY 1 cut(s) 415
SacI GAGCTC 2 cut(s) 107, 230
SapI GCTCTTC 2 cut(s) 95, 422
SatI GCNGC 2 cut(s) 129, 288
Sau96I GGNCC 3 cut(s) 315, 415, 453
ScrFI CCNGG 1 cut(s) 319
SduI GDGCHC 2 cut(s) 107, 230
SetI ASST 6 cut(s) 77, 107, 230, 317, 400, 417
SinI GGWCC 2 cut(s) 315, 415
Sse9I AATT 1 cut(s) 9
SsiI CCGC 5 cut(s) 120, 134, 186, 192, 409
SstI GAGCTC 2 cut(s) 107, 230
StyD4I CCNGG 1 cut(s) 317
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 2 cut(s) 77, 400
TaqI TCGA 2 cut(s) 46, 334
TasI AATT 1 cut(s) 9
TscAI CASTG 2 cut(s) 158, 373
TseI GCWGC 2 cut(s) 128, 287
TspDTI ATGAA 2 cut(s) 151, 401
TspGWI ACGGA 2 cut(s) 97, 338
TspRI CASTG 2 cut(s) 158, 373
VpaK11BI GGWCC 2 cut(s) 315, 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.