MD10G1067400.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008821
Physical Location & Seq
Reverse (-)
9297823 .. 9298278
456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1067400.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGCGTGGTCGCCGTACCTTTCCACTCGAGCCTCCACACCCTGTGCTTCTCTCACATTCCTCATCGGCTCTACAACAAGGAAAAGCTTCGTGTCTCTCAGCGGTGGCGCCGCCGCGCGTTCCCCGTTTCTTCATTGCTCCTTCATCTCTTCCTCCTCTATCGCTTCCACTTCCGCCTTCTCATGTTTATCTTTAGGTTTAGATTTGAGCTCTAGTGTTAGGATTGGAAGAAGAAGAGGCTCTGGCTTAGTGGTGAGGGCCGGGAAGGTTGCCCTATGTCTAACCAAGAGAAGTAAGTCCAGGAAATCCTTGGCTCGAACTCATGGATTCTATAGGCGCATGAGAACAACTGGAGGGAAAGCAATGATGAAGCACCGATATGCTAAGGGGCTGAAGGTCCTCTACACGAAGAGCAATCCCAGCAGTGGAAAACAGCCAGCACCGCCGATCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

152

Amino Acids

16.15

Weight (kDa)

11.73

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 104 - 134 9.1e-06 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccII CGCG 2 cut(s) 116, 118
AciI CCGC 5 cut(s) 102, 111, 114, 174, 443
AclWI GGATC 1 cut(s) 442
AcuI CTGAAG 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 44
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 425
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 2 cut(s) 87, 210
AluI AGCT 2 cut(s) 87, 210
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 27
AoxI GGCC 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 86
AspLEI GCGC 3 cut(s) 110, 118, 339
AspS9I GGNCC 2 cut(s) 258, 397
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 1 cut(s) 397
BanI GGYRCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 212
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
Bbv12I GWGCWC 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 2 cut(s) 111, 114
BlsI GCNGC 2 cut(s) 112, 115
Bme1390I CCNGG 2 cut(s) 262, 301
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 258, 397
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 2 cut(s) 262, 301
BpmI CTGGAG 1 cut(s) 372
Bpu10I CCTNAGC 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 309
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 133, 369
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 2 cut(s) 133, 369
BseMII CTCAG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 419
Bsh1236I CGCG 2 cut(s) 116, 118
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 1 cut(s) 99
BsnI GGCC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 1 cut(s) 447
BspACI CCGC 5 cut(s) 102, 111, 114, 174, 443
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 111
BspFNI CGCG 2 cut(s) 116, 118
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 442
BspQI GCTCTTC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 133, 369
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 1 cut(s) 447
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 301
Bst6I CTCTTC 3 cut(s) 154, 229, 404
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 98, 247, 384
BstFNI CGCG 2 cut(s) 116, 118
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 3 cut(s) 110, 118, 339
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 1 cut(s) 447
BstMWI GCNNNNNNNGC 2 cut(s) 420, 442
BstNI CCWGG 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 260, 299
BstSFI CTRYAG 1 cut(s) 331
BstUI CGCG 2 cut(s) 116, 118
BsuRI GGCC 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 438
CfoI GCGC 3 cut(s) 110, 118, 339
Cfr13I GGNCC 2 cut(s) 258, 397
Csp6I GTAC 1 cut(s) 16
CviAII CATG 3 cut(s) 183, 323, 340
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 98, 247, 384
DinI GGCGCC 1 cut(s) 109
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
DraIII CACNNNGTG 1 cut(s) 44
Eam1104I CTCTTC 3 cut(s) 154, 229, 404
EarI CTCTTC 3 cut(s) 154, 229, 404
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 413
Eco88I CYCGRG 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 210
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 212
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 3 cut(s) 186, 326, 343
FaiI YATR 6 cut(s) 184, 277, 324, 333, 341, 381
FatI CATG 3 cut(s) 182, 322, 339
Fnu4HI GCNGC 2 cut(s) 111, 114
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 2 cut(s) 111, 114
FspBI CTAG 1 cut(s) 213
GlaI GCGC 3 cut(s) 109, 117, 338
GluI GCNGC 2 cut(s) 111, 114
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 1 cut(s) 260
HapII CCGG 1 cut(s) 261
HhaI GCGC 3 cut(s) 110, 118, 339
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 3 cut(s) 186, 326, 343
Hin6I GCGC 3 cut(s) 108, 116, 337
HinP1I GCGC 3 cut(s) 108, 116, 337
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 1 cut(s) 327
HpaII CCGG 1 cut(s) 261
HphI GGTGA 1 cut(s) 265
HpyAV CCTTC 4 cut(s) 151, 187, 259, 388
HpyF10VI GCNNNNNNNGC 2 cut(s) 420, 442
HpyF3I CTNAG 3 cut(s) 98, 247, 384
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 3 cut(s) 186, 326, 343
HspAI GCGC 3 cut(s) 108, 116, 337
KasI GGCGCC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 447
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 8 cut(s) 55, 228, 274, 286, 313, 336, 433, 450
MaeI CTAG 1 cut(s) 213
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 6 cut(s) 122, 141, 240, 243, 246, 421
MhlI GDGCHC 1 cut(s) 212
Mly113I GGCGCC 1 cut(s) 108
MnlI CCTC 8 cut(s) 43, 71, 163, 166, 230, 249, 347, 410
MroXI GAANNNNTTC 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 2 cut(s) 262, 301
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 2 cut(s) 116, 118
MwoI GCNNNNNNNGC 2 cut(s) 420, 442
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 1 cut(s) 262
NdeII GATC 1 cut(s) 447
NlaIII CATG 3 cut(s) 186, 326, 343
NlaIV GGNNCC 1 cut(s) 109
PaeR7I CTCGAG 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 404
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 327
PfoI TCCNGGA 1 cut(s) 299
PkrI GCNGC 2 cut(s) 112, 115
PluTI GGCGCC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 212
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 2 cut(s) 258, 397
PspPPI RGGWCCY 1 cut(s) 397
PspXI VCTCGAGB 1 cut(s) 27
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 404
SatI GCNGC 2 cut(s) 111, 114
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 2 cut(s) 258, 397
ScrFI CCNGG 2 cut(s) 262, 301
SduI GDGCHC 1 cut(s) 212
SetI ASST 6 cut(s) 21, 89, 199, 212, 270, 399
SfcI CTRYAG 1 cut(s) 331
SfoI GGCGCC 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 1 cut(s) 27
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
SsiI CCGC 5 cut(s) 102, 111, 114, 174, 443
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 260, 299
StyI CCWWGG 1 cut(s) 309
TaqI TCGA 2 cut(s) 28, 316
TauI GCSGC 2 cut(s) 113, 116
TfiI GAWTC 1 cut(s) 327
TscAI CASTG 1 cut(s) 430
TspDTI ATGAA 3 cut(s) 122, 133, 383
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 27
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.