MD16G1032400.v1.1

ribosomal protein L34

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008821
Physical Location & Seq
Forward (+)
2321783 .. 2324932
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1032400.v1.1.491

Sequence Viewer

Length: 813 bp
ATGTTCTTAAATGGTGAGCCACGTGGGGCCCACCTTATGATTTTTTTGGTGTTTCCCGGTCTCTCTCTCTTCTCTCCTTCATTTTCTTTTTCCCTCTCCCGTAACTCTCTCGGCCCTCTCTATCTCAACCCCTCCGAGAGTTTTCTTACCAACAGACATCACCATCATCTTTATCTCGTTGCTACGAACTCAACCATAGTCTTCGTATCTTGGAAATCGAACCTGGAAGCCTCGTACACGAGCTCCGACGCAGACCGGCGACTTTCGAGGCCATTTCCGGCCAAACAACGGCGAGTTGGCCGGCGTGCAAGGTGTCGGCCAGCACGTGTCTATCCTGGTATTTGGGGAATATCAAGGTCGGGGTGTGTCAGTGGTCGCCGTACCTTTCCACTCGATCCTCCATACCCTATGCTTTTCTCACATTCCTCACTAGCTCCACAACTAGGAAGAGCTCTATGTCTCTCAGCGGTGGCGCCACCGCTTGTTCCCCGTTGCTTCACTGCTCCTTCATCTCTTTCACCTCTTTTGCTTCCATTTCCGCCTTCTCAGTTGAAATGTTTATCTTTGGGCTTGGATTCGAGCTCTAGTGTGGAGGTTAGAAGAAGAAGAGGCTGTGGCTTAGTGGTGAGGACCGGGAAGGCTGCCCTGTGTCTAATCAAGAGAAGTAGGTCCAGGAAATCCTTGGTTCAAACTCATGGATTCCGTTGCCGCATGAGAACAACTGGCGGGAGAGCAATGATGAAGCGCCAACGTGCTAAGGGGCGGAAGGTCCTCTGCACGAAAAGCAATTCCAACTGTGGAAAACGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34

Protein Analysis

271

Amino Acids

29.96

Weight (kDa)

11.64

Isoelectric Point (pI)

61.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34 PF00468 230 - 259 1.9e-07 Ribosomal protein L34
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 472
AciI CCGC 6 cut(s) 467, 479, 539, 709, 726, 763
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 3 cut(s) 279, 298, 317
AcvI CACGTG 2 cut(s) 23, 326
AcyI GRCGYC 1 cut(s) 473
AfaI GTAC 2 cut(s) 236, 382
AfiI CCNNNNNNNGG 2 cut(s) 288, 443
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 2 cut(s) 553, 689
AjnI CCWGG 3 cut(s) 222, 334, 671
AluBI AGCT 4 cut(s) 243, 434, 452, 582
AluI AGCT 4 cut(s) 243, 434, 452, 582
Alw21I GWGCWC 3 cut(s) 245, 454, 584
Alw26I GTCTC 2 cut(s) 65, 464
AlwI GGATC 1 cut(s) 389
AoxI GGCC 6 cut(s) 27, 112, 269, 279, 298, 317
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 1 cut(s) 641
AspLEI GCGC 2 cut(s) 475, 747
AspS9I GGNCC 6 cut(s) 27, 28, 113, 630, 669, 769
AsuC2I CCSGG 2 cut(s) 57, 634
AsuHPI GGTGA 4 cut(s) 26, 152, 510, 637
AvaII GGWCC 3 cut(s) 630, 669, 769
BaeGI GKGCMC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 4 cut(s) 31, 245, 454, 584
BauI CACGAG 1 cut(s) 238
BbrPI CACGTG 2 cut(s) 23, 326
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 3 cut(s) 245, 454, 584
BbvI GCAGC 1 cut(s) 628
BccI CCATC 1 cut(s) 171
BceAI ACGGC 2 cut(s) 305, 363
BciT130I CCWGG 3 cut(s) 224, 336, 673
BcnI CCSGG 2 cut(s) 57, 634
BcoDI GTCTC 2 cut(s) 65, 464
BfaI CTAG 3 cut(s) 431, 443, 585
BfoI RGCGCY 2 cut(s) 476, 748
BisI GCNGC 2 cut(s) 642, 709
BlsI GCNGC 2 cut(s) 643, 710
Bme1390I CCNGG 5 cut(s) 57, 224, 336, 634, 673
Bme18I GGWCC 3 cut(s) 630, 669, 769
BmgT120I GGNCC 6 cut(s) 27, 28, 113, 630, 669, 769
BmiI GGNNCC 3 cut(s) 28, 29, 474
BmrFI CCNGG 5 cut(s) 57, 224, 336, 634, 673
BpiI GAAGAC 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 756
BpuMI CCSGG 2 cut(s) 57, 634
BsaAI YACGTR 2 cut(s) 23, 326
BsaHI GRCGYC 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 681
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 443
Bse118I RCCGGY 2 cut(s) 255, 300
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 3 cut(s) 224, 336, 673
BseDI CCNNGG 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 288, 443
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 2 cut(s) 477, 560
BseNI ACTGG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 1 cut(s) 628
BsgI GTGCAG 1 cut(s) 760
BshFI GGCC 6 cut(s) 29, 114, 271, 281, 300, 319
BshNI GGYRCC 1 cut(s) 472
BsiHKAI GWGCWC 3 cut(s) 245, 454, 584
BsiSI CCGG 5 cut(s) 57, 256, 278, 301, 633
BslI CCNNNNNNNGG 2 cut(s) 288, 443
BsmAI GTCTC 2 cut(s) 65, 464
BsnI GGCC 6 cut(s) 29, 114, 271, 281, 300, 319
Bso31I GGTCTC 1 cut(s) 65
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 4 cut(s) 31, 245, 454, 584
Bsp143I GATC 1 cut(s) 394
BspACI CCGC 6 cut(s) 467, 479, 539, 709, 726, 763
BspANI GGCC 6 cut(s) 29, 114, 271, 281, 300, 319
BspCNI CTCAG 2 cut(s) 476, 559
BspLI GGNNCC 3 cut(s) 28, 29, 474
BspPI GGATC 1 cut(s) 389
BspQI GCTCTTC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 741
BsrFI RCCGGY 2 cut(s) 255, 300
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 2 cut(s) 255, 300
BssECI CCNNGG 1 cut(s) 681
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 473
BssSI CACGAG 1 cut(s) 238
BssT1I CCWWGG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 238
Bst2UI CCWGG 3 cut(s) 224, 336, 673
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 3 cut(s) 74, 442, 601
BstACI GRCGYC 1 cut(s) 473
BstBAI YACGTR 2 cut(s) 23, 326
BstC8I GCNNGC 3 cut(s) 302, 306, 321
BstDEI CTNAG 4 cut(s) 463, 546, 619, 756
BstH2I RGCGCY 2 cut(s) 476, 748
BstHHI GCGC 2 cut(s) 475, 747
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 2 cut(s) 65, 464
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 1 cut(s) 783
BstNI CCWGG 3 cut(s) 224, 336, 673
BstSCI CCNGG 5 cut(s) 55, 222, 334, 632, 671
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 628
BstV2I GAAGAC 1 cut(s) 193
BsuRI GGCC 6 cut(s) 29, 114, 271, 281, 300, 319
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 376, 498
Cac8I GCNNGC 3 cut(s) 302, 306, 321
CfoI GCGC 2 cut(s) 475, 747
Cfr10I RCCGGY 2 cut(s) 255, 300
Cfr13I GGNCC 6 cut(s) 27, 28, 113, 630, 669, 769
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 235, 381
CviAII CATG 2 cut(s) 695, 712
CviQI GTAC 2 cut(s) 235, 381
DdeI CTNAG 4 cut(s) 463, 546, 619, 756
DinI GGCGCC 1 cut(s) 474
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 279, 298, 317
Eam1104I CTCTTC 3 cut(s) 74, 442, 601
EarI CTCTTC 3 cut(s) 74, 442, 601
EciI GGCGGA 2 cut(s) 528, 778
Ecl136II GAGCTC 3 cut(s) 243, 452, 582
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 4 cut(s) 31, 245, 454, 584
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 3 cut(s) 630, 669, 769
Eco53kI GAGCTC 3 cut(s) 243, 452, 582
Eco72I CACGTG 2 cut(s) 23, 326
EcoICRI GAGCTC 3 cut(s) 243, 452, 582
EcoO109I RGGNCCY 2 cut(s) 27, 769
EcoRII CCWGG 3 cut(s) 222, 334, 671
EcoT14I CCWWGG 1 cut(s) 681
EcoT38I GRGCYC 4 cut(s) 31, 245, 454, 584
EgeI GGCGCC 1 cut(s) 474
EheI GGCGCC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 2 cut(s) 698, 715
FaiI YATR 7 cut(s) 38, 197, 403, 410, 457, 696, 713
FatI CATG 2 cut(s) 694, 711
FauI CCCGC 1 cut(s) 719
Fnu4HI GCNGC 2 cut(s) 642, 709
FriOI GRGCYC 4 cut(s) 31, 245, 454, 584
Fsp4HI GCNGC 2 cut(s) 642, 709
FspBI CTAG 3 cut(s) 431, 443, 585
GlaI GCGC 2 cut(s) 474, 746
GluI GCNGC 2 cut(s) 642, 709
HaeII RGCGCY 2 cut(s) 476, 748
HaeIII GGCC 6 cut(s) 29, 114, 271, 281, 300, 319
HapII CCGG 5 cut(s) 57, 256, 278, 301, 633
HgaI GACGC 1 cut(s) 257
HhaI GCGC 2 cut(s) 475, 747
Hin1I GRCGYC 1 cut(s) 473
Hin1II CATG 2 cut(s) 698, 715
Hin6I GCGC 2 cut(s) 473, 745
HinP1I GCGC 2 cut(s) 473, 745
HinfI GANTC 2 cut(s) 575, 699
HpaII CCGG 5 cut(s) 57, 256, 278, 301, 633
HphI GGTGA 4 cut(s) 26, 152, 510, 637
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 2 cut(s) 136, 247
Hpy188III TCNNGA 1 cut(s) 658
Hpy8I GTNNAC 1 cut(s) 237
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 5 cut(s) 87, 516, 552, 631, 760
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 4 cut(s) 22, 325, 751, 805
HpyCH4V TGCA 2 cut(s) 308, 777
HpyF10VI GCNNNNNNNGC 1 cut(s) 783
HpyF3I CTNAG 4 cut(s) 463, 546, 619, 756
HpySE526I ACGT 4 cut(s) 22, 325, 751, 805
Hsp92I GRCGYC 1 cut(s) 473
Hsp92II CATG 2 cut(s) 698, 715
HspAI GCGC 2 cut(s) 473, 745
KasI GGCGCC 1 cut(s) 472
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 1 cut(s) 394
LguI GCTCTTC 1 cut(s) 442
LmnI GCTCC 3 cut(s) 248, 439, 508
Lsp1109I GCAGC 1 cut(s) 628
MaeI CTAG 3 cut(s) 431, 443, 585
MaeII ACGT 4 cut(s) 22, 325, 751, 805
MaeIII GTNAC 1 cut(s) 101
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 6 cut(s) 61, 193, 459, 612, 615, 618
MhlI GDGCHC 4 cut(s) 31, 245, 454, 584
MluCI AATT 1 cut(s) 787
Mly113I GGCGCC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 300
MseI TTAA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 5 cut(s) 57, 256, 278, 301, 633
MspR9I CCNGG 5 cut(s) 57, 224, 336, 634, 673
MvaI CCWGG 3 cut(s) 224, 336, 673
MwoI GCNNNNNNNGC 1 cut(s) 783
NaeI GCCGGC 1 cut(s) 302
NarI GGCGCC 1 cut(s) 473
NciI CCSGG 2 cut(s) 57, 634
NdeII GATC 1 cut(s) 394
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 2 cut(s) 698, 715
NlaIV GGNNCC 3 cut(s) 28, 29, 474
NmeAIII GCCGAG 1 cut(s) 90
PciSI GCTCTTC 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PdiI GCCGGC 1 cut(s) 302
PfeI GAWTC 2 cut(s) 575, 699
PfoI TCCNGGA 1 cut(s) 671
PkrI GCNGC 2 cut(s) 643, 710
PluTI GGCGCC 1 cut(s) 476
PmaCI CACGTG 2 cut(s) 23, 326
PmlI CACGTG 2 cut(s) 23, 326
Ppu21I YACGTR 2 cut(s) 23, 326
PpuMI RGGWCCY 1 cut(s) 769
Psp124BI GAGCTC 3 cut(s) 245, 454, 584
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 3 cut(s) 222, 334, 671
PspCI CACGTG 2 cut(s) 23, 326
PspGI CCWGG 3 cut(s) 222, 334, 671
PspN4I GGNNCC 3 cut(s) 28, 29, 474
PspOMI GGGCCC 1 cut(s) 27
PspPI GGNCC 6 cut(s) 27, 28, 113, 630, 669, 769
PspPPI RGGWCCY 1 cut(s) 769
RsaI GTAC 2 cut(s) 236, 382
RsaNI GTAC 2 cut(s) 235, 381
SacI GAGCTC 3 cut(s) 245, 454, 584
SapI GCTCTTC 1 cut(s) 442
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 2 cut(s) 642, 709
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 6 cut(s) 27, 28, 113, 630, 669, 769
ScrFI CCNGG 5 cut(s) 57, 224, 336, 634, 673
SduI GDGCHC 4 cut(s) 31, 245, 454, 584
SfoI GGCGCC 1 cut(s) 474
SinI GGWCC 3 cut(s) 630, 669, 769
Sse9I AATT 1 cut(s) 787
SsiI CCGC 6 cut(s) 467, 479, 539, 709, 726, 763
SspDI GGCGCC 1 cut(s) 472
SspMI CTAG 3 cut(s) 431, 443, 585
SstI GAGCTC 3 cut(s) 245, 454, 584
StyD4I CCNGG 5 cut(s) 55, 222, 334, 632, 671
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 4 cut(s) 25, 328, 754, 808
TaqI TCGA 4 cut(s) 218, 266, 393, 578
TasI AATT 1 cut(s) 787
TauI GCSGC 1 cut(s) 711
TfiI GAWTC 2 cut(s) 575, 699
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 376, 505
TseI GCWGC 1 cut(s) 641
TspDTI ATGAA 3 cut(s) 69, 498, 755
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 2 cut(s) 376, 505
VpaK11BI GGWCC 3 cut(s) 630, 669, 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XspI CTAG 3 cut(s) 431, 443, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.